Sgr025397 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr025397
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionVacuolar-processing enzyme
Locationtig00004836: 2163457 .. 2165525 (+)
RNA-Seq ExpressionSgr025397
SyntenySgr025397
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCCGACGTAGTCACATTTCCACACTTGAGATGGCAACTTATGATGCAAGTTCAACTAAACTTGTTCTTCTCATAGCTCTTTTGTATTTCAATAATATTATCGCTAAGAAAGAGTGGGTTGGTGGTGCCACTATTGAGCAACCCGGCAAGAGATGGGCGCTTCTGGTTGCTGGCTCCAATGGTTATGATAATTACAGGCATCAGGTGCCCTTATACAAATCAATTACACAGCCCCGCACCCACATATTGATATTAACTTCTTTTTTTTTTTGACATAAAAAAACAAAAACCTAGGCGGATGTATGCCATGCATATCAAATACTGAGAAAAGGTGGCCTCCCCGATGACAACATAATAGTTTTCATGTATGACGATATTGCATTCCATCCGAGCAATCCAAGGCCTGGAGTCATTATCAACAAACCGAACGGCGTTGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAGAGCATGTGAATGCAAACAACTTTTACGCTGCCATTTTGGGCAACAAATCTGCTCTTACTGGAGGAAGTGGTAAGGTCGTCGATAGTGGTCCTCATGATCATATTTTCATCTACTACACCGATCATGGTAGTGCTGGGATACTTGGTAAGTTACACTTACTACTCTTTTTTGTCTCAACATATTCATTTGATGTTAGAAATTTGACAATATTACTTCACATAATCATAGGAATGCCCGAGGGCGACTATGTTTACGTCAAGGATCTAATAGACATGCTGAAAAAAAAGCATGAAGCCAAAAGTTACAAAAGCATGGTACCTACATTCCATATAAAAGCATAGAAGTTATGAAGTTCAAATTATCAATGTAAAATAACAAGTTAACATAAACATTCAGGTAATATATGTGGAAGCATGTGAGTCAGGGAGTATGTTTGAAGGACTTCTTCCAGAAAACATAAAGATATATGCGACAACAGCATCAAATGCAACAGAGAATAGCTGGGCAACATACTGTCCTGGACAATTTCCCAGTCCTCCCATAGGTTATGATACTTGTCTTGGAGATTTGTACAGCATCGCTTGGATGGAAGACAGGCAATATTTTCTTTATATCCCTGCCTGTATAATAAAGCTAACCTGTACCTAGTCAACCCCAACCATATTAAAATGAATTTGTGTTGTGCCATGACATGCAGTGACAAACATGATTTGAGCAAGGAAACTTTGAGCCAACAATATCATGTGGTACACATTTTAATCCCTAAATGAGCTCAATTTTGTTAAATGACATGATAAATGTATGTTTGTACATTGCCAAAATGCAGGTTCGAAGAAGAACTTCGGTTGATAACTTCGGTTATGGTTCTCATGTAATGTTGTATGGAAACAAAAGCATAGGCAACAATTTCCTTGACACTTACATTGGTACTAACCCTGATAATTACAACTATACATTCTCTGTCCATAGTAGTCACACCATCTCTCCACCATCAAAATTGTTATCCTCAAATGCTGTTAGCCAGCGTGATGCTAGTCTCATTCATTACTGGCATAAGGTATGGCTTCTTAAACAACATATAAAGCTTTTCTAATCATGATTGCAAACTGCTAGATAGTTTAAACGTGTAGACTTTGATAATAATTATCAGTTTCAAAAAGCTCCATTTGGCTCTAGAAAAAAGATGGAGGCTAGAAAGCAGCTAGAGGATGAAATTTTGAATAGAAGGCATGTTGACTATAATATCAATCACATTGGTAAACTTTTGTTTGGACAAGCAAAGAGCTCAAAAGTACTCAACGATGTTAGACAGCAAGGACAATCCTTCATTGATGATTGGGACTGCTTTAAGAACTTTGTAAGTCCTATTTTCAAGCCCTACCTAGCTATATTTTATATCATTAAAAACTCTGCTGATGCAATCTCTAAAACACAGGTGAAAACCTATGAGAAACATTGCAGAAGACTGTCAAGATATGGGATGAAATACACACGAGCTCTAGCTAATATATGCAATGCTGGGATTACAATGAACCAAATGGATCAGGCTTGTCTGCAAACTTGCCTTGTTAAATTTAACTTGAAACTAGATTAG

mRNA sequence

ATGCTCCGACGTAGTCACATTTCCACACTTGAGATGGCAACTTATGATGCAAGTTCAACTAAACTTGTTCTTCTCATAGCTCTTTTGTATTTCAATAATATTATCGCTAAGAAAGAGTGGGTTGGTGGTGCCACTATTGAGCAACCCGGCAAGAGATGGGCGCTTCTGGTTGCTGGCTCCAATGGTTATGATAATTACAGGCATCAGGCGGATGTATGCCATGCATATCAAATACTGAGAAAAGGTGGCCTCCCCGATGACAACATAATAGTTTTCATGTATGACGATATTGCATTCCATCCGAGCAATCCAAGGCCTGGAGTCATTATCAACAAACCGAACGGCGTTGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAGAGCATGTGAATGCAAACAACTTTTACGCTGCCATTTTGGGCAACAAATCTGCTCTTACTGGAGGAAGTGGTAAGGTCGTCGATAGTGGTCCTCATGATCATATTTTCATCTACTACACCGATCATGGTAGTGCTGGGATACTTGGAATGCCCGAGGGCGACTATGTTTACGTCAAGGATCTAATAGACATGCTGAAAAAAAAGCATGAAGCCAAAAGTTACAAAAGCATGGTAATATATGTGGAAGCATGTGAGTCAGGGAGTATGTTTGAAGGACTTCTTCCAGAAAACATAAAGATATATGCGACAACAGCATCAAATGCAACAGAGAATAGCTGGGCAACATACTGTCCTGGACAATTTCCCAGTCCTCCCATAGGTTATGATACTTGTCTTGGAGATTTGTACAGCATCGCTTGGATGGAAGACAGTGACAAACATGATTTGAGCAAGGAAACTTTGAGCCAACAATATCATGTGGTTCGAAGAAGAACTTCGGTTGATAACTTCGGTTATGGTTCTCATGTAATGTTGTATGGAAACAAAAGCATAGGCAACAATTTCCTTGACACTTACATTGGTACTAACCCTGATAATTACAACTATACATTCTCTGTCCATAGTAGTCACACCATCTCTCCACCATCAAAATTGTTATCCTCAAATGCTGTTAGCCAGCGTGATGCTAGTCTCATTCATTACTGGCATAAGTTTCAAAAAGCTCCATTTGGCTCTAGAAAAAAGATGGAGGCTAGAAAGCAGCTAGAGGATGAAATTTTGAATAGAAGGCATGTTGACTATAATATCAATCACATTGGTAAACTTTTGTTTGGACAAGCAAAGAGCTCAAAAGTACTCAACGATGTTAGACAGCAAGGACAATCCTTCATTGATGATTGGGACTGCTTTAAGAACTTTGTGAAAACCTATGAGAAACATTGCAGAAGACTGTCAAGATATGGGATGAAATACACACGAGCTCTAGCTAATATATGCAATGCTGGGATTACAATGAACCAAATGGATCAGGCTTGTCTGCAAACTTGCCTTGTTAAATTTAACTTGAAACTAGATTAG

Coding sequence (CDS)

ATGCTCCGACGTAGTCACATTTCCACACTTGAGATGGCAACTTATGATGCAAGTTCAACTAAACTTGTTCTTCTCATAGCTCTTTTGTATTTCAATAATATTATCGCTAAGAAAGAGTGGGTTGGTGGTGCCACTATTGAGCAACCCGGCAAGAGATGGGCGCTTCTGGTTGCTGGCTCCAATGGTTATGATAATTACAGGCATCAGGCGGATGTATGCCATGCATATCAAATACTGAGAAAAGGTGGCCTCCCCGATGACAACATAATAGTTTTCATGTATGACGATATTGCATTCCATCCGAGCAATCCAAGGCCTGGAGTCATTATCAACAAACCGAACGGCGTTGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAGAGCATGTGAATGCAAACAACTTTTACGCTGCCATTTTGGGCAACAAATCTGCTCTTACTGGAGGAAGTGGTAAGGTCGTCGATAGTGGTCCTCATGATCATATTTTCATCTACTACACCGATCATGGTAGTGCTGGGATACTTGGAATGCCCGAGGGCGACTATGTTTACGTCAAGGATCTAATAGACATGCTGAAAAAAAAGCATGAAGCCAAAAGTTACAAAAGCATGGTAATATATGTGGAAGCATGTGAGTCAGGGAGTATGTTTGAAGGACTTCTTCCAGAAAACATAAAGATATATGCGACAACAGCATCAAATGCAACAGAGAATAGCTGGGCAACATACTGTCCTGGACAATTTCCCAGTCCTCCCATAGGTTATGATACTTGTCTTGGAGATTTGTACAGCATCGCTTGGATGGAAGACAGTGACAAACATGATTTGAGCAAGGAAACTTTGAGCCAACAATATCATGTGGTTCGAAGAAGAACTTCGGTTGATAACTTCGGTTATGGTTCTCATGTAATGTTGTATGGAAACAAAAGCATAGGCAACAATTTCCTTGACACTTACATTGGTACTAACCCTGATAATTACAACTATACATTCTCTGTCCATAGTAGTCACACCATCTCTCCACCATCAAAATTGTTATCCTCAAATGCTGTTAGCCAGCGTGATGCTAGTCTCATTCATTACTGGCATAAGTTTCAAAAAGCTCCATTTGGCTCTAGAAAAAAGATGGAGGCTAGAAAGCAGCTAGAGGATGAAATTTTGAATAGAAGGCATGTTGACTATAATATCAATCACATTGGTAAACTTTTGTTTGGACAAGCAAAGAGCTCAAAAGTACTCAACGATGTTAGACAGCAAGGACAATCCTTCATTGATGATTGGGACTGCTTTAAGAACTTTGTGAAAACCTATGAGAAACATTGCAGAAGACTGTCAAGATATGGGATGAAATACACACGAGCTCTAGCTAATATATGCAATGCTGGGATTACAATGAACCAAATGGATCAGGCTTGTCTGCAAACTTGCCTTGTTAAATTTAACTTGAAACTAGATTAG

Protein sequence

MLRRSHISTLEMATYDASSTKLVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLIHYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTCLVKFNLKLD
Homology
[Close]
BLAST of Sgr025397 vs. NCBI nr
Match: XP_022148051.1 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 [Momordica charantia])

HSP 1 Score: 846.7 bits (2186), Expect = 1.0e-241
Identity = 407/469 (86.78%), Postives = 436/469 (92.96%), Query Frame = 0 

Query: 12  MATYDASSTKLVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGSNGYDNYRHQAD 71
           MAT  A+STK VLLIALL F++IIAK+E V GA+ +QPGKRWA+LVAGS+GY+NYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 72  VCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEHV 131
           VCHAYQILRKGGLPD+NIIVFMYDDIAF+PSNPRPGV+INKP+GVDVYQGVPKDYTGEHV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 132 NANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLID 191
           N+ NFYA ILGN+SALTGGSGKVVDS  HDHIFIYYTDHGSAG+LGMPEGDYVY KDL++
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 192 MLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFPS 251
           +LK+KHEAKSYKSMVIYVEACESGSM EGLLPENIKIYATTASNATENSWATYCPGQFPS
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 252 PPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNKS 311
           PP  YDTCLGDLYSIAWMEDSDKHDLSKETL QQY  VRRRT VD FGYGSHVMLYGNKS
Sbjct: 241 PPTDYDTCLGDLYSIAWMEDSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKS 300

Query: 312 IGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLIHYWHKFQKAPF 371
           IGNN LDTYIG NPDNYNYT SV S+ TI+PPSKLL SNAVSQRDASLIHYWHKFQKAPF
Sbjct: 301 IGNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPF 360

Query: 372 GSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDCF 431
           GSR+K EARKQLEDEILNRRHVD +I HI KLLFGQAKSS+VLN+VRQQGQS +DDW CF
Sbjct: 361 GSREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCF 420

Query: 432 KNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTCLVK 481
           K FVKTYEKHCRRLSRYGMKYTRALANICNAGIT+NQMDQACL+TCLVK
Sbjct: 421 KKFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETCLVK 469

[Close]
BLAST of Sgr025397 vs. NCBI nr
Match: XP_022148043.1 (vacuolar-processing enzyme-like isoform X1 [Momordica charantia])

HSP 1 Score: 829.3 bits (2141), Expect = 1.7e-236
Identity = 407/503 (80.91%), Postives = 436/503 (86.68%), Query Frame = 0 

Query: 12  MATYDASSTKLVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGSNGYDNYRHQAD 71
           MAT  A+STK VLLIALL F++IIAK+E V GA+ +QPGKRWA+LVAGS+GY+NYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 72  VCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEHV 131
           VCHAYQILRKGGLPD+NIIVFMYDDIAF+PSNPRPGV+INKP+GVDVYQGVPKDYTGEHV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 132 NANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLID 191
           N+ NFYA ILGN+SALTGGSGKVVDS  HDHIFIYYTDHGSAG+LGMPEGDYVY KDL++
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 192 MLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFPS 251
           +LK+KHEAKSYKSMVIYVEACESGSM EGLLPENIKIYATTASNATENSWATYCPGQFPS
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 252 PPIGYDTCLGDLYSIAWMED----------------------------------SDKHDL 311
           PP  YDTCLGDLYSIAWMED                                  SDKHDL
Sbjct: 241 PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL 300

Query: 312 SKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSS 371
           SKETL QQY  VRRRT VD FGYGSHVMLYGNKSIGNN LDTYIG NPDNYNYT SV S+
Sbjct: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360

Query: 372 HTISPPSKLLSSNAVSQRDASLIHYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNI 431
            TI+PPSKLL SNAVSQRDASLIHYWHKFQKAPFGSR+K EARKQLEDEILNRRHVD +I
Sbjct: 361 DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI 420

Query: 432 NHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALA 481
            HI KLLFGQAKSS+VLN+VRQQGQS +DDW CFK FVKTYEKHCRRLSRYGMKYTRALA
Sbjct: 421 YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA 480

[Close]
BLAST of Sgr025397 vs. NCBI nr
Match: XP_038880044.1 (vacuolar-processing enzyme-like [Benincasa hispida])

HSP 1 Score: 763.8 bits (1971), Expect = 8.7e-217
Identity = 370/471 (78.56%), Postives = 413/471 (87.69%), Query Frame = 0 

Query: 12  MATYDASSTK-LVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGSNGYDNYRHQA 71
           M T + S TK LV+LI  ++FNNIIAKKEW+  AT  Q GKRWALLVAGSNGY+NYRHQA
Sbjct: 1   MTTCNRSPTKLLVILIIFVFFNNIIAKKEWISDATANQTGKRWALLVAGSNGYENYRHQA 60

Query: 72  DVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEH 131
           DVCHAYQIL+KGG+PD+NIIVFMYDDIAFHP+NPR G+IINKP G DVYQGVPKDYTG+ 
Sbjct: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPNNPRQGIIINKPGGPDVYQGVPKDYTGKQ 120

Query: 132 VNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLI 191
           VNA NFYAA+ GNKSALTGGSGKV+ SGPHDHIFIYY+DHG+AG+LGMPE DYV+V DLI
Sbjct: 121 VNAVNFYAALSGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPEDDYVFVNDLI 180

Query: 192 DMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFP 251
           D LK+KH+AKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATE+SWATYCP +  
Sbjct: 181 DALKRKHKAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATEDSWATYCPKESR 240

Query: 252 S-PPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGYGSHVMLYGN 311
             PP  Y+TCLGDLYS+AWMEDSDKHDLSKETL+QQYH VRRRT VD  G GSHVMLYGN
Sbjct: 241 GPPPKDYNTCLGDLYSVAWMEDSDKHDLSKETLNQQYHAVRRRTLVDKSGVGSHVMLYGN 300

Query: 312 KSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLIHYWHKFQKA 371
           + IG +FL+T+IG +PD  NY  SV S HTIS PSK LSSN VSQRDASLI+Y +KFQKA
Sbjct: 301 RRIGTDFLNTFIGNSPDYNNYKSSVQSIHTISSPSK-LSSNGVSQRDASLIYYQYKFQKA 360

Query: 372 PFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWD 431
           P GSR+KMEAR+QLEDEIL RRH D +IN+IGKLLFGQA+SSKVLN VR+QGQS +DDW 
Sbjct: 361 PIGSREKMEAREQLEDEILKRRHADNSINNIGKLLFGQARSSKVLNKVREQGQSLVDDWH 420

Query: 432 CFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTCLVK 481
           CFK FVK YEKHC+R+S YGMKYTRA+ANICNAGITM+QMDQACLQTCL K
Sbjct: 421 CFKTFVKIYEKHCKRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCLGK 470

[Close]
BLAST of Sgr025397 vs. NCBI nr
Match: XP_022961313.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita moschata])

HSP 1 Score: 760.4 bits (1962), Expect = 9.6e-216
Identity = 359/478 (75.10%), Postives = 417/478 (87.24%), Query Frame = 0 

Query: 1   MLRRSHISTLEMATYDASSTKLVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGS 60
           +L+ +HIS+  M T+  + T L LLIALL+FN+IIAKK+WV  A  +QPGKRWALLVAGS
Sbjct: 8   ILKHTHISSPNMTTFATTPTTL-LLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGS 67

Query: 61  NGYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQ 120
           NGYDNYRHQADVCHAYQILRK G+PD+NIIVFMYDDIA HP NPRPG+IINKP G DVYQ
Sbjct: 68  NGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQ 127

Query: 121 GVPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPE 180
           GVPKDYTG+ VN+ NFYAAILGNKS +TGGSGKV+DSGP+DHIFIYYTDHG+AG+LGMP 
Sbjct: 128 GVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPM 187

Query: 181 GDYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENS 240
           GDY+Y  DL+D+LK+KHEAKSYKSMVIYVEACE+GS+FEGLLPEN+ IYATTASNATE+S
Sbjct: 188 GDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDS 247

Query: 241 WATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGY 300
           WA YCPGQ P+P  GYDTCLGDLYS+AWMEDSDKHD SKETL+QQYH VRRRTSVD  GY
Sbjct: 248 WAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGY 307

Query: 301 GSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLI 360
           GSHVMLYGN+SIGNNFL+TY G   D+ N   SV S+H IS       SN VSQRDASL+
Sbjct: 308 GSHVMLYGNESIGNNFLNTYFGNTSDDNNNESSVKSTHIISS-----LSNGVSQRDASLL 367

Query: 361 HYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQ 420
           HYW+KFQ AP+GSR+KMEA ++LEDEIL+RRH D ++N IG+LLFG+AKSS+VLN +R  
Sbjct: 368 HYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSVNQIGELLFGEAKSSRVLNRIRGH 427

Query: 421 GQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTCL 479
           GQSF+DDW+CFK +VK YEKHC+R+SRYGMKYT+ALANICNAGIT++QMD+ACLQTC+
Sbjct: 428 GQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITIDQMDKACLQTCV 478

[Close]
BLAST of Sgr025397 vs. NCBI nr
Match: XP_023516171.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 756.1 bits (1951), Expect = 1.8e-214
Identity = 359/467 (76.87%), Postives = 410/467 (87.79%), Query Frame = 0 

Query: 12  MATYDASSTKLVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGSNGYDNYRHQAD 71
           M T+  + T L LLIALL+FN+IIAKK+WV  A  +QPGKRWALLVAGSNGYDNYRHQAD
Sbjct: 1   MTTFATTPTTL-LLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQAD 60

Query: 72  VCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEHV 131
           VCHAYQILRK G+PD+NIIVFMYDDIAF P NPRPG+IINKP G DVYQGVPKDYTG+ V
Sbjct: 61  VCHAYQILRK-GIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDV 120

Query: 132 NANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLID 191
           N+ NFYAAILGNKS +TGGSGKV+DSGP+DHIFIYYTDHG+AG+LGMP GDY+Y  DL+D
Sbjct: 121 NSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMD 180

Query: 192 MLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFPS 251
           +LK+KHEAKSYKSMVIYVEACESGSMFEGLLPEN+ IYATTASNATE+SWA YCPGQ P+
Sbjct: 181 VLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPT 240

Query: 252 PPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNKS 311
           P  GYDTCLGDLYS+AWMEDSDKHD SKETL+QQYH VRRRTSVD  GYGSHVMLYGN+S
Sbjct: 241 PHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNES 300

Query: 312 IGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLIHYWHKFQKAPF 371
           IGNNFL+TY G + D+ N   SV S+H IS       +N VSQRDASL+HYW+KFQ AP+
Sbjct: 301 IGNNFLNTYFGNSSDDNNNESSVKSTHIISS-----LANGVSQRDASLLHYWYKFQNAPY 360

Query: 372 GSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDCF 431
           GSR+KMEA ++LEDEIL+RRH D +IN IG+LLFG+AKSS+VLN +R  GQSF+DDW+CF
Sbjct: 361 GSREKMEASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCF 420

Query: 432 KNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTCL 479
           K +VK YEKHC+R+SRYGMKYT+ALANICNAGITM+QMDQACLQTC+
Sbjct: 421 KKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCV 460

[Close]
BLAST of Sgr025397 vs. ExPASy Swiss-Prot
Match: P49043 (Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 3.0e-159
Identity = 270/440 (61.36%), Postives = 333/440 (75.68%), Query Frame = 0 

Query: 42  GGATI----EQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDI 101
           GGA +    +  G RWA+L+AGSNG+ NYRHQAD+CHAYQ+LRKGGL D+NIIVFMYDDI
Sbjct: 46  GGAKVNDDDDSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDI 105

Query: 102 AFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDS 161
           AF+  NPRPGVIIN P+G DVY+GVPKDYTGE V    F+A +LGNK+ALTGGSGKVVDS
Sbjct: 106 AFNEENPRPGVIINHPHGDDVYKGVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVVDS 165

Query: 162 GPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSM 221
           GP+DHIFI+Y+DHG  G+LGMP   Y+Y  +LID+LKKKH + +YKS+V Y+EACESGS+
Sbjct: 166 GPNDHIFIFYSDHGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESGSI 225

Query: 222 FEGLLPENIKIYATTASNATENSWATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDL 281
           FEGLL E + IYATTASNA E+SW TYCPG+ P PP  Y TCLGDLYSIAWMEDSD H+L
Sbjct: 226 FEGLLLEGLNIYATTASNAEESSWGTYCPGEIPGPPPEYSTCLGDLYSIAWMEDSDIHNL 285

Query: 282 SKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSS 341
             ETL QQY +V+ RT+  N  YGSHVM YG+  +  N L TY+GTNP N NYTF     
Sbjct: 286 RTETLHQQYELVKTRTASYN-SYGSHVMQYGDIGLSKNNLFTYLGTNPANDNYTFV--DE 345

Query: 342 HTISPPSKLLSSNAVSQRDASLIHYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNI 401
           +++ P SK     AV+QRDA L+H+W K++KAP G+ +K EA+KQ  + + +R HVD++I
Sbjct: 346 NSLRPASK-----AVNQRDADLLHFWDKYRKAPEGTPRKAEAQKQFFEAMSHRMHVDHSI 405

Query: 402 NHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALA 461
             IGKLLFG  K  ++LN VR  GQ  +DDW C K+ V+T+E HC  LS+YGMK+ R+LA
Sbjct: 406 KLIGKLLFGIEKGPEILNTVRPAGQPLVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLA 465

Query: 462 NICNAGITMNQMDQACLQTC 478
           NICN GI   +M +A  Q C
Sbjct: 466 NICNTGIGKEKMAEASAQAC 477

[Close]
BLAST of Sgr025397 vs. ExPASy Swiss-Prot
Match: Q39119 (Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g32940 PE=1 SV=2)

HSP 1 Score: 562.4 bits (1448), Expect = 5.1e-159
Identity = 258/428 (60.28%), Postives = 334/428 (78.04%), Query Frame = 0 

Query: 50  GKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVI 109
           G RWA+LVAGS+GY NYRHQAD+CHAYQ+LRKGGL ++NI+VFMYDDIA +  NPRPG I
Sbjct: 57  GTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTI 116

Query: 110 INKPNGVDVYQGVPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTD 169
           IN P+G DVYQGVPKDYTG+ VN +N +A ILG+K+A+ GGSGKVVDSGP+DHIFI+Y+D
Sbjct: 117 INSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSD 176

Query: 170 HGSAGILGMPEGDYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIY 229
           HG  G+LGMP   Y+Y  DL D+LKKKH   +YKS+V Y+EACESGS+FEGLLPE + IY
Sbjct: 177 HGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIY 236

Query: 230 ATTASNATENSWATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVV 289
           ATTASNA E+SW TYCPG+ PSPP  Y+TCLGDLYS+AWMEDS  H+L  ETL QQY +V
Sbjct: 237 ATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYELV 296

Query: 290 RRRTSVDNFGYGSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSS 349
           +RRT+   + YGSHVM YG+  I  + LD Y+GTNP N N+TF+   ++++ PPS++   
Sbjct: 297 KRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFA--DANSLKPPSRV--- 356

Query: 350 NAVSQRDASLIHYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAK 409
              +QRDA L+H+W K++KAP GS +K EA+KQ+ + + +R H+D ++  +GK+LFG ++
Sbjct: 357 --TNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISR 416

Query: 410 SSKVLNDVRQQGQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQM 469
             +VLN VR  GQ  +DDW+C KN V+ +E+HC  LS+YG+K+ R+ ANICNAGI M QM
Sbjct: 417 GPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQM 476

Query: 470 DQACLQTC 478
           ++A  Q C
Sbjct: 477 EEAASQAC 477

[Close]
BLAST of Sgr025397 vs. ExPASy Swiss-Prot
Match: O24325 (Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 8.6e-159
Identity = 273/477 (57.23%), Postives = 347/477 (72.75%), Query Frame = 0 

Query: 12  MATYDASSTKLVLLIALLYFNNIIAKKEWVGGATIEQP----------GKRWALLVAGSN 71
           MAT  A+++ L LL  LL+   +++    + G  +  P          G RWA+L AGS+
Sbjct: 1   MATTTATTSLLALL--LLFLVALVSAGRDLVGDFLRLPSDSGNGDNVHGTRWAILFAGSS 60

Query: 72  GYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQG 131
           GY NYRHQAD+CHAYQ+LRKGGL D+NIIVFMYDDIAF+  NPR GVIIN PNG +VY+G
Sbjct: 61  GYWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVIINSPNGDEVYKG 120

Query: 132 VPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEG 191
           VPKDYTGE V A+NFYAA+LG+KS LTGGSGKVV+SGP+DHIFI+Y+DHG  G+LG P G
Sbjct: 121 VPKDYTGEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAG 180

Query: 192 DYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSW 251
            Y+Y  DL ++LKKKH + +YK++V Y+EACESGS+FEGLLPE+I +YATTASNA E+SW
Sbjct: 181 PYIYASDLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVYATTASNADESSW 240

Query: 252 ATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGYG 311
            TYCPG+ PSPP  Y TCLGDLYS+AWMEDSD+H+L  ETL QQY +V+ RT      YG
Sbjct: 241 GTYCPGEDPSPPPEYSTCLGDLYSVAWMEDSDRHNLRTETLHQQYKLVKERTISGGLYYG 300

Query: 312 SHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLIH 371
           SHVM YG+  +  + L  Y+GT+P N N TF       +   S   SS AV+QRDA L+H
Sbjct: 301 SHVMQYGDVGLSKDILFHYLGTDPANENLTF-------VDENSLWSSSKAVNQRDADLVH 360

Query: 372 YWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQG 431
           +W KF+KAP GS KK EARKQ+ + + +R H+D ++  +GKLLFG  K+ ++LN VR  G
Sbjct: 361 FWDKFRKAPEGSPKKNEARKQVLEVMSHRMHIDDSVELVGKLLFGIEKAPELLNAVRPAG 420

Query: 432 QSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTCL 479
            + +DDWDC K  V+T+E HC  LS+YGMK+ R+ AN+CN GI   QM +A  Q C+
Sbjct: 421 SALVDDWDCLKTMVRTFETHCGSLSQYGMKHMRSFANMCNVGIKKEQMREASAQACV 468

[Close]
BLAST of Sgr025397 vs. ExPASy Swiss-Prot
Match: P49047 (Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g25940 PE=2 SV=2)

HSP 1 Score: 546.2 bits (1406), Expect = 3.7e-154
Identity = 254/426 (59.62%), Postives = 331/426 (77.70%), Query Frame = 0 

Query: 52  RWALLVAGSNGYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIIN 111
           +WA+LVAGS+GY NYRHQADVCHAYQ+L+KGG+ ++NI+VFMYDDIA +  NPRPGVIIN
Sbjct: 44  KWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRPGVIIN 103

Query: 112 KPNGVDVYQGVPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHG 171
            PNG DVY GVPKDYTG+ VN +N  A ILGNK+AL GGSGKVVDSGP+DHIFIYY+DHG
Sbjct: 104 SPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDHG 163

Query: 172 SAGILGMPEGDYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYAT 231
             G+LGMP    +Y  DL D+LKKK+ + +YKS+V Y+EACESGS+FEGLLPE + IYAT
Sbjct: 164 GPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYAT 223

Query: 232 TASNATENSWATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRR 291
           TASNA E+SW TYCPG+ PSPP  Y+TCLGDLYS+AW+EDS+KH+L  ETL +QY +V++
Sbjct: 224 TASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQYELVKK 283

Query: 292 RTSVDNFGYGSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNA 351
           RT+     YGSHVM +G+  +    L  ++GTNP + N+TF   + ++I PPS++     
Sbjct: 284 RTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFV--NENSIRPPSRV----- 343

Query: 352 VSQRDASLIHYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSS 411
            +QRDA L+H+WHK+QKAP GS +K+EA+KQ+ + + +R HVD +I  IG LLFG  +  
Sbjct: 344 TNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG-LEGH 403

Query: 412 KVLNDVRQQGQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQ 471
            VLN VR  G+  +DDWDC K+ V+ +E+HC  LS+YG+K+ R++AN+CNAGI M QM++
Sbjct: 404 AVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMRQMEE 461

Query: 472 ACLQTC 478
           A +Q C
Sbjct: 464 AAMQAC 461

[Close]
BLAST of Sgr025397 vs. ExPASy Swiss-Prot
Match: P49044 (Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 4.9e-154
Identity = 261/428 (60.98%), Postives = 326/428 (76.17%), Query Frame = 0 

Query: 50  GKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVI 109
           G RWA+L+AGSNGY NYRHQ+DVCHAYQ+LRKGG  ++NIIVFMYDDIA +  NPRPGVI
Sbjct: 54  GTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDIASNEENPRPGVI 113

Query: 110 INKPNGVDVYQGVPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTD 169
           INKP+G DVY GVPKDYTG  V+A+NFYAA+LGNKSALTGGSGKVVDSGP+DHIF+YYTD
Sbjct: 114 INKPDGDDVYAGVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHIFVYYTD 173

Query: 170 HGSAGILGMPEGDYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIY 229
           HG  G+LGMP G Y+Y  DL ++LKKKH + +YKS+V Y+EACESGS+FEGLLP+++ IY
Sbjct: 174 HGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPDDLNIY 233

Query: 230 ATTASNATENSWATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVV 289
           ATTASNA E+SW  YCPG  P PP  Y TCLGDLYSIAWMEDS+ H+L  E+L QQY +V
Sbjct: 234 ATTASNAEESSWGYYCPGDKPPPPPEYSTCLGDLYSIAWMEDSEVHNLQTESLQQQYKLV 293

Query: 290 RRRTSVDNFGYGSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSS 349
           + RT  +   YGSHVM YG+  +  N L  Y+GTNP N N +F   + +++   +    S
Sbjct: 294 KNRTISE--PYGSHVMEYGDIGLSKNDLYQYLGTNPANDNNSFVDETENSLKLRT---PS 353

Query: 350 NAVSQRDASLIHYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAK 409
            AV+QRDA LIH+W KF+KAP GS +K EA KQ+ + + +R+H+D ++  IG+LLFG  K
Sbjct: 354 AAVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVLEAMSHRKHIDNSVKLIGQLLFGIEK 413

Query: 410 SSKVLNDVRQQGQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQM 469
            +++L+ VR  G   +D+WDC K  VKT+E HC  LS+YGMK+ R+ ANICNAGI    M
Sbjct: 414 GTELLDVVRPAGSPLVDNWDCLKTMVKTFETHCGSLSQYGMKHMRSFANICNAGIPNEPM 473

Query: 470 DQACLQTC 478
            +A  Q C
Sbjct: 474 AEASAQAC 476

[Close]
BLAST of Sgr025397 vs. ExPASy TrEMBL
Match: A0A6J1D475 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 5.0e-242
Identity = 407/469 (86.78%), Postives = 436/469 (92.96%), Query Frame = 0 

Query: 12  MATYDASSTKLVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGSNGYDNYRHQAD 71
           MAT  A+STK VLLIALL F++IIAK+E V GA+ +QPGKRWA+LVAGS+GY+NYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 72  VCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEHV 131
           VCHAYQILRKGGLPD+NIIVFMYDDIAF+PSNPRPGV+INKP+GVDVYQGVPKDYTGEHV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 132 NANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLID 191
           N+ NFYA ILGN+SALTGGSGKVVDS  HDHIFIYYTDHGSAG+LGMPEGDYVY KDL++
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 192 MLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFPS 251
           +LK+KHEAKSYKSMVIYVEACESGSM EGLLPENIKIYATTASNATENSWATYCPGQFPS
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 252 PPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNKS 311
           PP  YDTCLGDLYSIAWMEDSDKHDLSKETL QQY  VRRRT VD FGYGSHVMLYGNKS
Sbjct: 241 PPTDYDTCLGDLYSIAWMEDSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKS 300

Query: 312 IGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLIHYWHKFQKAPF 371
           IGNN LDTYIG NPDNYNYT SV S+ TI+PPSKLL SNAVSQRDASLIHYWHKFQKAPF
Sbjct: 301 IGNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPF 360

Query: 372 GSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDCF 431
           GSR+K EARKQLEDEILNRRHVD +I HI KLLFGQAKSS+VLN+VRQQGQS +DDW CF
Sbjct: 361 GSREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCF 420

Query: 432 KNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTCLVK 481
           K FVKTYEKHCRRLSRYGMKYTRALANICNAGIT+NQMDQACL+TCLVK
Sbjct: 421 KKFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETCLVK 469

[Close]
BLAST of Sgr025397 vs. ExPASy TrEMBL
Match: A0A6J1D2Z4 (vacuolar-processing enzyme-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 8.2e-237
Identity = 407/503 (80.91%), Postives = 436/503 (86.68%), Query Frame = 0 

Query: 12  MATYDASSTKLVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGSNGYDNYRHQAD 71
           MAT  A+STK VLLIALL F++IIAK+E V GA+ +QPGKRWA+LVAGS+GY+NYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 72  VCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEHV 131
           VCHAYQILRKGGLPD+NIIVFMYDDIAF+PSNPRPGV+INKP+GVDVYQGVPKDYTGEHV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 132 NANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLID 191
           N+ NFYA ILGN+SALTGGSGKVVDS  HDHIFIYYTDHGSAG+LGMPEGDYVY KDL++
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 192 MLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFPS 251
           +LK+KHEAKSYKSMVIYVEACESGSM EGLLPENIKIYATTASNATENSWATYCPGQFPS
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 252 PPIGYDTCLGDLYSIAWMED----------------------------------SDKHDL 311
           PP  YDTCLGDLYSIAWMED                                  SDKHDL
Sbjct: 241 PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL 300

Query: 312 SKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSS 371
           SKETL QQY  VRRRT VD FGYGSHVMLYGNKSIGNN LDTYIG NPDNYNYT SV S+
Sbjct: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360

Query: 372 HTISPPSKLLSSNAVSQRDASLIHYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNI 431
            TI+PPSKLL SNAVSQRDASLIHYWHKFQKAPFGSR+K EARKQLEDEILNRRHVD +I
Sbjct: 361 DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI 420

Query: 432 NHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALA 481
            HI KLLFGQAKSS+VLN+VRQQGQS +DDW CFK FVKTYEKHCRRLSRYGMKYTRALA
Sbjct: 421 YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA 480

[Close]
BLAST of Sgr025397 vs. ExPASy TrEMBL
Match: A0A6J1HBF5 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=LOC111461846 PE=3 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 4.7e-216
Identity = 359/478 (75.10%), Postives = 417/478 (87.24%), Query Frame = 0 

Query: 1   MLRRSHISTLEMATYDASSTKLVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGS 60
           +L+ +HIS+  M T+  + T L LLIALL+FN+IIAKK+WV  A  +QPGKRWALLVAGS
Sbjct: 8   ILKHTHISSPNMTTFATTPTTL-LLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGS 67

Query: 61  NGYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQ 120
           NGYDNYRHQADVCHAYQILRK G+PD+NIIVFMYDDIA HP NPRPG+IINKP G DVYQ
Sbjct: 68  NGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQ 127

Query: 121 GVPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPE 180
           GVPKDYTG+ VN+ NFYAAILGNKS +TGGSGKV+DSGP+DHIFIYYTDHG+AG+LGMP 
Sbjct: 128 GVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPM 187

Query: 181 GDYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENS 240
           GDY+Y  DL+D+LK+KHEAKSYKSMVIYVEACE+GS+FEGLLPEN+ IYATTASNATE+S
Sbjct: 188 GDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDS 247

Query: 241 WATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGY 300
           WA YCPGQ P+P  GYDTCLGDLYS+AWMEDSDKHD SKETL+QQYH VRRRTSVD  GY
Sbjct: 248 WAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGY 307

Query: 301 GSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLI 360
           GSHVMLYGN+SIGNNFL+TY G   D+ N   SV S+H IS       SN VSQRDASL+
Sbjct: 308 GSHVMLYGNESIGNNFLNTYFGNTSDDNNNESSVKSTHIISS-----LSNGVSQRDASLL 367

Query: 361 HYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQ 420
           HYW+KFQ AP+GSR+KMEA ++LEDEIL+RRH D ++N IG+LLFG+AKSS+VLN +R  
Sbjct: 368 HYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSVNQIGELLFGEAKSSRVLNRIRGH 427

Query: 421 GQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTCL 479
           GQSF+DDW+CFK +VK YEKHC+R+SRYGMKYT+ALANICNAGIT++QMD+ACLQTC+
Sbjct: 428 GQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITIDQMDKACLQTCV 478

[Close]
BLAST of Sgr025397 vs. ExPASy TrEMBL
Match: A0A6J1JF37 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC111485183 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.4e-214
Identity = 360/467 (77.09%), Postives = 405/467 (86.72%), Query Frame = 0 

Query: 12  MATYDASSTKLVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGSNGYDNYRHQAD 71
           M T+  + T L LLI LL+FN+IIAKKEWV  A  +QPGKRWALLVAGSNGYDNYRHQAD
Sbjct: 1   MTTFATTPTTL-LLIPLLFFNSIIAKKEWVSHANTDQPGKRWALLVAGSNGYDNYRHQAD 60

Query: 72  VCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEHV 131
           VCHAYQILRK G+PD+NIIVFMYDDIAFHP NPRPG+IINKP G DVY GVPKDYTG+ V
Sbjct: 61  VCHAYQILRK-GIPDENIIVFMYDDIAFHPDNPRPGIIINKPGGADVYHGVPKDYTGKDV 120

Query: 132 NANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLID 191
           N+ NFYAAILGNKS +TGGSGKV+DSGP+DHIFIYYTDHG+AG+LGMP GDY+Y  DL+D
Sbjct: 121 NSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMD 180

Query: 192 MLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFPS 251
           +LK+KHEAKSYKSMVIYVEACESGSMFEGLLPEN+ IYATTASNATE+SWA YCPGQ P+
Sbjct: 181 VLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPT 240

Query: 252 PPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNKS 311
           P  GYDTCLGDLYS+AWMEDSDKHD SKETL+QQYH VRRRTSVD  GYGSHVMLYGN+S
Sbjct: 241 PHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNES 300

Query: 312 IGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLIHYWHKFQKAPF 371
            GNNFL+TY G   D+ N   SV S+H IS       SNAVSQRDASL+HYW+KFQ AP+
Sbjct: 301 FGNNFLNTYFGNTSDDNNNESSVKSTHIISS-----LSNAVSQRDASLLHYWYKFQNAPY 360

Query: 372 GSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDCF 431
           GSR+KMEA +QLEDEIL+RRH D +IN IG LLFG+AKSS+VL   R  GQSF+DDW+CF
Sbjct: 361 GSREKMEASEQLEDEILSRRHADNSINQIGDLLFGEAKSSRVLYRSRGHGQSFVDDWNCF 420

Query: 432 KNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTCL 479
           KN+VK YEKHC+R+SRYGMKYT+ALAN CNAGITM+QMDQACLQTC+
Sbjct: 421 KNYVKIYEKHCKRMSRYGMKYTQALANTCNAGITMDQMDQACLQTCV 460

[Close]
BLAST of Sgr025397 vs. ExPASy TrEMBL
Match: A0A5A7TXA7 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00680 PE=3 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 8.0e-208
Identity = 355/470 (75.53%), Postives = 402/470 (85.53%), Query Frame = 0 

Query: 12  MATYDASSTK-LVLLIALLYFNNIIAKKEWVGGATIEQPGKRWALLVAGSNGYDNYRHQA 71
           M T D + TK L++LI  L FNNIIAK EW+  AT +Q GK WALL+AGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 72  DVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEH 131
                      GG+PD+NIIVFMYDDIAFHPSNPRPG+IINKP+G DVY GVPKDYTG+H
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 132 VNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLI 191
           VNA NFYAAILGNKSALTGGSGKV+ SGP DHIFIYY+DHG+AG+LGMPEGDY++V DLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 192 DMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFP 251
           D+LK+KH+AK+YKSMVIYVEACESGSMFEG+LPENIKIYATTASNATE+SWATYCP Q  
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 252 SPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNK 311
             P  Y+TCLGDL+S+AWMEDSDKHDLSKETL+QQYH VRRRT VD  GYGSHVMLYGNK
Sbjct: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300

Query: 312 SIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLIHYWHKFQKAP 371
            IGN+FLDT++G +PD  NY +SV S  TIS  SK LSSN+VSQRDASLIHY +KFQ+AP
Sbjct: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSK-LSSNSVSQRDASLIHYQYKFQEAP 360

Query: 372 FGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDC 431
           FGSR+KMEAR+QLEDEI  RRH D +IN IGKLLFGQA+SS++L++VR QGQS +DDW C
Sbjct: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420

Query: 432 FKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTCLVK 481
           FK FVK YEKHC R+S YGMKYTRA+ANICNAGITM+QMDQACLQTCL K
Sbjct: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCLGK 457

[Close]
BLAST of Sgr025397 vs. TAIR 10
Match: AT4G32940.1 (gamma vacuolar processing enzyme )

HSP 1 Score: 562.4 bits (1448), Expect = 3.6e-160
Identity = 258/428 (60.28%), Postives = 334/428 (78.04%), Query Frame = 0 

Query: 50  GKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVI 109
           G RWA+LVAGS+GY NYRHQAD+CHAYQ+LRKGGL ++NI+VFMYDDIA +  NPRPG I
Sbjct: 57  GTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTI 116

Query: 110 INKPNGVDVYQGVPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTD 169
           IN P+G DVYQGVPKDYTG+ VN +N +A ILG+K+A+ GGSGKVVDSGP+DHIFI+Y+D
Sbjct: 117 INSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSD 176

Query: 170 HGSAGILGMPEGDYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIY 229
           HG  G+LGMP   Y+Y  DL D+LKKKH   +YKS+V Y+EACESGS+FEGLLPE + IY
Sbjct: 177 HGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIY 236

Query: 230 ATTASNATENSWATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVV 289
           ATTASNA E+SW TYCPG+ PSPP  Y+TCLGDLYS+AWMEDS  H+L  ETL QQY +V
Sbjct: 237 ATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYELV 296

Query: 290 RRRTSVDNFGYGSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSS 349
           +RRT+   + YGSHVM YG+  I  + LD Y+GTNP N N+TF+   ++++ PPS++   
Sbjct: 297 KRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFA--DANSLKPPSRV--- 356

Query: 350 NAVSQRDASLIHYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAK 409
              +QRDA L+H+W K++KAP GS +K EA+KQ+ + + +R H+D ++  +GK+LFG ++
Sbjct: 357 --TNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISR 416

Query: 410 SSKVLNDVRQQGQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQM 469
             +VLN VR  GQ  +DDW+C KN V+ +E+HC  LS+YG+K+ R+ ANICNAGI M QM
Sbjct: 417 GPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQM 476

Query: 470 DQACLQTC 478
           ++A  Q C
Sbjct: 477 EEAASQAC 477

[Close]
BLAST of Sgr025397 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 546.2 bits (1406), Expect = 2.7e-155
Identity = 254/426 (59.62%), Postives = 331/426 (77.70%), Query Frame = 0 

Query: 52  RWALLVAGSNGYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIIN 111
           +WA+LVAGS+GY NYRHQADVCHAYQ+L+KGG+ ++NI+VFMYDDIA +  NPRPGVIIN
Sbjct: 44  KWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRPGVIIN 103

Query: 112 KPNGVDVYQGVPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHG 171
            PNG DVY GVPKDYTG+ VN +N  A ILGNK+AL GGSGKVVDSGP+DHIFIYY+DHG
Sbjct: 104 SPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDHG 163

Query: 172 SAGILGMPEGDYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIYAT 231
             G+LGMP    +Y  DL D+LKKK+ + +YKS+V Y+EACESGS+FEGLLPE + IYAT
Sbjct: 164 GPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYAT 223

Query: 232 TASNATENSWATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRR 291
           TASNA E+SW TYCPG+ PSPP  Y+TCLGDLYS+AW+EDS+KH+L  ETL +QY +V++
Sbjct: 224 TASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQYELVKK 283

Query: 292 RTSVDNFGYGSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNA 351
           RT+     YGSHVM +G+  +    L  ++GTNP + N+TF   + ++I PPS++     
Sbjct: 284 RTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFV--NENSIRPPSRV----- 343

Query: 352 VSQRDASLIHYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSS 411
            +QRDA L+H+WHK+QKAP GS +K+EA+KQ+ + + +R HVD +I  IG LLFG  +  
Sbjct: 344 TNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG-LEGH 403

Query: 412 KVLNDVRQQGQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQ 471
            VLN VR  G+  +DDWDC K+ V+ +E+HC  LS+YG+K+ R++AN+CNAGI M QM++
Sbjct: 404 AVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMRQMEE 461

Query: 472 ACLQTC 478
           A +Q C
Sbjct: 464 AAMQAC 461

[Close]
BLAST of Sgr025397 vs. TAIR 10
Match: AT3G20210.1 (delta vacuolar processing enzyme )

HSP 1 Score: 499.6 bits (1285), Expect = 2.9e-141
Identity = 249/461 (54.01%), Postives = 312/461 (67.68%), Query Frame = 0 

Query: 22  LVLLIALLYFNNIIAKKEWVGGATIEQ-----PGKRWALLVAGSNGYDNYRHQADVCHAY 81
           LV L ALL F+    K + +    +E       G RWA+LVAGSN Y NYRHQAD+CHAY
Sbjct: 11  LVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAY 70

Query: 82  QILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEHVNANNF 141
           QILRKGGL D+NIIVFMYDDIAF   NPRPGVIINKP+G DVY+GVPKDYT E VN  NF
Sbjct: 71  QILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNF 130

Query: 142 YAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLIDMLKKK 201
           Y  +LGN+S +TGG+GKVV SGP+D+IFIYY DHG+ G++ MP GD V  KD  ++L+K 
Sbjct: 131 YNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKM 190

Query: 202 HEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFPSPPIGY 261
           H+ K Y  MVIYVEACESGSMFEG+L +N+ IYA TA+N+ E+SW  YCP  +P PP   
Sbjct: 191 HKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEI 250

Query: 262 DTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNKSIGNNF 321
            TCLGD +SI+W+EDSD HD+SKETL QQYHVV+RR   D     SHV  +G + +  ++
Sbjct: 251 GTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSD-VPETSHVCRFGTEKMLKDY 310

Query: 322 LDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLIHYWHKFQKAPFGSRKK 381
           L +YIG NP+N N+TF+   S  IS      +S  V+ RD  L++   K QKAP GS + 
Sbjct: 311 LSSYIGRNPENDNFTFTESFSSPIS------NSGLVNPRDIPLLYLQRKIQKAPMGSLES 370

Query: 382 MEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDCFKNFVK 441
            EA+K+L DE  +R+ +D +I  I +L   Q     +L   R  GQ  +DDWDCFK  V 
Sbjct: 371 KEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLVN 430

Query: 442 TYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQTC 478
           +++ HC     YG+KYT ALANICN G+ + Q   A  Q C
Sbjct: 431 SFKNHCGATVHYGLKYTGALANICNMGVDVKQTVSAIEQAC 464

[Close]
BLAST of Sgr025397 vs. TAIR 10
Match: AT3G20210.2 (delta vacuolar processing enzyme )

HSP 1 Score: 496.5 bits (1277), Expect = 2.4e-140
Identity = 248/459 (54.03%), Postives = 311/459 (67.76%), Query Frame = 0 

Query: 22  LVLLIALLYFNNIIAKKEWVGGATIEQ-----PGKRWALLVAGSNGYDNYRHQADVCHAY 81
           LV L ALL F+    K + +    +E       G RWA+LVAGSN Y NYRHQAD+CHAY
Sbjct: 11  LVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAY 70

Query: 82  QILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVIINKPNGVDVYQGVPKDYTGEHVNANNF 141
           QILRKGGL D+NIIVFMYDDIAF   NPRPGVIINKP+G DVY+GVPKDYT E VN  NF
Sbjct: 71  QILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNF 130

Query: 142 YAAILGNKSALTGGSGKVVDSGPHDHIFIYYTDHGSAGILGMPEGDYVYVKDLIDMLKKK 201
           Y  +LGN+S +TGG+GKVV SGP+D+IFIYY DHG+ G++ MP GD V  KD  ++L+K 
Sbjct: 131 YNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKM 190

Query: 202 HEAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATENSWATYCPGQFPSPPIGY 261
           H+ K Y  MVIYVEACESGSMFEG+L +N+ IYA TA+N+ E+SW  YCP  +P PP   
Sbjct: 191 HKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEI 250

Query: 262 DTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVVRRRTSVDNFGYGSHVMLYGNKSIGNNF 321
            TCLGD +SI+W+EDSD HD+SKETL QQYHVV+RR   D     SHV  +G + +  ++
Sbjct: 251 GTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSD-VPETSHVCRFGTEKMLKDY 310

Query: 322 LDTYIGTNPDNYNYTFSVHSSHTISPPSKLLSSNAVSQRDASLIHYWHKFQKAPFGSRKK 381
           L +YIG NP+N N+TF+   S  IS      +S  V+ RD  L++   K QKAP GS + 
Sbjct: 311 LSSYIGRNPENDNFTFTESFSSPIS------NSGLVNPRDIPLLYLQRKIQKAPMGSLES 370

Query: 382 MEARKQLEDEILNRRHVDYNINHIGKLLFGQAKSSKVLNDVRQQGQSFIDDWDCFKNFVK 441
            EA+K+L DE  +R+ +D +I  I +L   Q     +L   R  GQ  +DDWDCFK  V 
Sbjct: 371 KEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLVN 430

Query: 442 TYEKHCRRLSRYGMKYTRALANICNAGITMNQMDQACLQ 476
           +++ HC     YG+KYT ALANICN G+ + Q   A  Q
Sbjct: 431 SFKNHCGATVHYGLKYTGALANICNMGVDVKQTVSAIEQ 462

[Close]
BLAST of Sgr025397 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 493.4 bits (1269), Expect = 2.0e-139
Identity = 235/429 (54.78%), Postives = 305/429 (71.10%), Query Frame = 0 

Query: 50  GKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLPDDNIIVFMYDDIAFHPSNPRPGVI 109
           G RWA+LVAGS+GY NYRHQADVCHAYQILRKGGL ++NI+V MYDDIA HP NPRPG +
Sbjct: 49  GTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTL 108

Query: 110 INKPNGVDVYQGVPKDYTGEHVNANNFYAAILGNKSALTGGSGKVVDSGPHDHIFIYYTD 169
           IN P+G DVY GVPKDYTG  V A NFYA +LG++ A+ GGSGKV+ S P+DHIF+YY D
Sbjct: 109 INHPDGDDVYAGVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYAD 168

Query: 170 HGSAGILGMPEGDYVYVKDLIDMLKKKHEAKSYKSMVIYVEACESGSMFEGLLPENIKIY 229
           HG  G+LGMP   ++Y  D I+ LKKKH + +YK MVIYVEACESGS+FEG++P+++ IY
Sbjct: 169 HGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIY 228

Query: 230 ATTASNATENSWATYCPGQFPSPPIGYDTCLGDLYSIAWMEDSDKHDLSKETLSQQYHVV 289
            TTASNA E+S+ TYCPG  PSPP  Y TCLGDLYS+AWMEDS+ H+L KET+ QQYH V
Sbjct: 229 VTTASNAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSETHNLKKETIKQQYHTV 288

Query: 290 RRRTS-VDNFGYGSHVMLYGNKSIGNNFLDTYIGTNPDNYNYTFSVHSSHTISPPSKLLS 349
           + RTS  + +  GSHVM YGN SI +  L  Y G +P   N        + +   SK+  
Sbjct: 289 KMRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGFDPATVNLPL-----NELPVKSKI-- 348

Query: 350 SNAVSQRDASLIHYWHKFQKAPFGSRKKMEARKQLEDEILNRRHVDYNINHIGKLLFGQA 409
              V+QRDA L+  WH ++ +  GSRKK +  K+L +   +R+H+D ++  I  +LFG  
Sbjct: 349 -GVVNQRDADLLFLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLDASVELIATILFG-- 408

Query: 410 KSSKVLNDVRQQGQSFIDDWDCFKNFVKTYEKHCRRLSRYGMKYTRALANICNAGITMNQ 469
            +  VLN VR+ G   +DDW+C K+ V+ +E+HC  L++YGMK+ RA AN+CN G++   
Sbjct: 409 PTMNVLNLVREPGLPLVDDWECLKSMVRVFEEHCGSLTQYGMKHMRAFANVCNNGVSKEL 467

Query: 470 MDQACLQTC 478
           M++A    C
Sbjct: 469 MEEASTAAC 467

The following BLAST results are available for this feature:
BLAST of Sgr025397 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022148051.11.0e-24186.78vacuolar-processing enzyme gamma-isozyme-like isoform X2 [Momordica charantia][more]
XP_022148043.11.7e-23680.91vacuolar-processing enzyme-like isoform X1 [Momordica charantia][more]
XP_038880044.18.7e-21778.56vacuolar-processing enzyme-like [Benincasa hispida][more]
XP_022961313.19.6e-21675.10vacuolar-processing enzyme gamma-isozyme-like [Cucurbita moschata][more]
XP_023516171.11.8e-21476.87vacuolar-processing enzyme gamma-isozyme-like [Cucurbita pepo subsp. pepo][more]
BLAST of Sgr025397 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P490433.0e-15961.36Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1[more]
Q391195.1e-15960.28Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
O243258.6e-15957.23Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1[more]
P490473.7e-15459.62Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g... [more]
P490444.9e-15460.98Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1[more]
BLAST of Sgr025397 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1D4755.0e-24286.78vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia ... [more]
A0A6J1D2Z48.2e-23780.91vacuolar-processing enzyme-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1HBF54.7e-21675.10vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JF374.4e-21477.09vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5A7TXA78.0e-20875.53Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=119... [more]
BLAST of Sgr025397 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G32940.13.6e-16060.28gamma vacuolar processing enzyme [more]
AT2G25940.12.7e-15559.62alpha-vacuolar processing enzyme [more]
AT3G20210.12.9e-14154.01delta vacuolar processing enzyme [more]
AT3G20210.22.4e-14054.03delta vacuolar processing enzyme [more]
AT1G62710.12.0e-13954.78beta vacuolar processing enzyme [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001096Peptidase C13, legumainPRINTSPR00776HEMOGLOBNASEcoord: 153..172
score: 61.52
coord: 78..107
score: 58.41
coord: 125..140
score: 51.9
coord: 53..77
score: 75.48
coord: 202..218
score: 67.26
IPR001096Peptidase C13, legumainPIRSFPIRSF019663Legumaincoord: 15..484
e-value: 2.8E-209
score: 693.8
IPR001096Peptidase C13, legumainPFAMPF01650Peptidase_C13coord: 52..322
e-value: 1.1E-110
score: 369.1
IPR001096Peptidase C13, legumainPANTHERPTHR12000HEMOGLOBINASE FAMILY MEMBERcoord: 41..479
NoneNo IPR availableGENE3D1.10.132.130coord: 354..483
e-value: 5.7E-37
score: 128.6
NoneNo IPR availableGENE3D3.40.50.1460coord: 50..345
e-value: 1.9E-127
score: 426.6
NoneNo IPR availablePIRSRPIRSR019663-1PIRSR019663-1coord: 50..478
e-value: 3.0E-201
score: 667.2
NoneNo IPR availablePANTHERPTHR12000:SF45VACUOLAR-PROCESSING ENZYME-LIKEcoord: 41..479
IPR043577Asparaginyl endopeptidasePIRSFPIRSF500139AEcoord: 11..484
e-value: 2.0E-210
score: 697.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr025397.1Sgr025397.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006624 vacuolar protein processing
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005773 vacuole
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008233 peptidase activity