Homology
BLAST of Sgr025368 vs. NCBI nr
Match:
XP_038899448.1 ((R)-mandelonitrile lyase-like [Benincasa hispida])
HSP 1 Score: 938.3 bits (2424), Expect = 3.0e-269
Identity = 467/556 (83.99%), Postives = 500/556 (89.93%), Query Frame = 0
Query: 1 MARRNFFYSQCLAFLVVFYGAISISANPKSLQQDLVTEPSYLKFVSNATAFPVEDYYDYI 60
MA R FF+SQ LAFL++FY + A+P S Q+ EP YLKFV+NAT FP EDYYDYI
Sbjct: 19 MAPRFFFHSQFLAFLLIFYASF---AHPNSSQR----EPDYLKFVANATEFPEEDYYDYI 78
Query: 61 IVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSPVQAF 120
IVGGGTAGCPLAATLSS FRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVH+RDSPVQAF
Sbjct: 79 IVGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRDSPVQAF 138
Query: 121 TSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEIVFRP 180
TSEEGVPNARGRILGGSSAINAGFYSRADQ FF+NS +NWD+ +VNQSYEWVEKEIVFRP
Sbjct: 139 TSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSRLNWDLAMVNQSYEWVEKEIVFRP 198
Query: 181 NIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYATPFNI 240
N+KNWQSAVRD MVE GINPYNGFSLDH++GTKIGGSTFD++GRR SAADLL+YATP NI
Sbjct: 199 NLKNWQSAVRDGMVEVGINPYNGFSLDHLIGTKIGGSTFDELGRRHSAADLLSYATPINI 258
Query: 241 KVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAMLRARGEVLVSAGAIGSPQ 300
KVAVYATVERILLAS R +AIGVLYRDQ YHHAMLRARGEVLVSAGAIGSPQ
Sbjct: 259 KVAVYATVERILLASGGPL--RRTTAIGVLYRDQKGAYHHAMLRARGEVLVSAGAIGSPQ 318
Query: 301 LLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES 360
LLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES
Sbjct: 319 LLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES 378
Query: 361 GAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEKIIGPLSSGSMRLASTDIRVNP 420
GAYLEAASNVIPFTSPPRSFFIRTPSAP+YLTVATLMEKI GP+SSGSMRLASTD+RVNP
Sbjct: 379 GAYLEAASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKITGPVSSGSMRLASTDVRVNP 438
Query: 421 IVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFVGPALPVDEAND 480
+VRFNYF N VDLERCVNGTRKIG++LRSRS+E FKF DW GR +FRFVGP P +E D
Sbjct: 439 VVRFNYFGNAVDLERCVNGTRKIGEILRSRSMEGFKFRDWLGRSEFRFVGPVYPENEDED 498
Query: 481 G-LMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQATL 540
G ++ +FCRRSVSTIWHYHGGCV GKVVD N RVIGMEALRVVDGS F VSPGTNPQATL
Sbjct: 499 GSMLREFCRRSVSTIWHYHGGCVAGKVVDSNSRVIGMEALRVVDGSIFGVSPGTNPQATL 558
Query: 541 MMLGRYVGLQIIKERS 556
MMLGR+VGLQII +RS
Sbjct: 559 MMLGRHVGLQIINQRS 565
BLAST of Sgr025368 vs. NCBI nr
Match:
KGN45431.1 (hypothetical protein Csa_016192 [Cucumis sativus])
HSP 1 Score: 914.1 bits (2361), Expect = 6.0e-262
Identity = 455/556 (81.83%), Postives = 492/556 (88.49%), Query Frame = 0
Query: 1 MARRNFFYSQCLAFLVVFYGAISISANPKSLQQDLVTEPSYLKFVSNATAFPVEDYYDYI 60
M R F+SQ L FL++F + SA P S QQD P YLKFVSNAT FP DYYDYI
Sbjct: 1 MESRISFHSQILTFLLIFSAS---SALPNSSQQD----PDYLKFVSNATEFPEVDYYDYI 60
Query: 61 IVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSPVQAF 120
IVGGGTAGCPLAATLSS FRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVH+R+SPVQAF
Sbjct: 61 IVGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAF 120
Query: 121 TSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEIVFRP 180
TSEEGVPNARGRILGGSSAINAGFYSRADQ FF+NS + WD+ IVNQSYEWVEKEIVF+P
Sbjct: 121 TSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKP 180
Query: 181 NIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYATPFNI 240
N+KNWQSAVRD MVEAGI PYNGFSLDHV+GTKIGGSTFD++GRR SAADLL++ATPFNI
Sbjct: 181 NLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSHATPFNI 240
Query: 241 KVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAMLRARGEVLVSAGAIGSPQ 300
KVA+YA VERILLAST R AIGVLYRDQ YHHAML ARGEV +SAGAIGSPQ
Sbjct: 241 KVAIYANVERILLASTVK---RRPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQ 300
Query: 301 LLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES 360
LLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES
Sbjct: 301 LLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES 360
Query: 361 GAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEKIIGPLSSGSMRLASTDIRVNP 420
G YLEAASNVIPFTSPPRSFFIRTPS P+YLTVATLMEKI GP++SGSMRLASTD+RVNP
Sbjct: 361 GTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNP 420
Query: 421 IVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFVGPALPVDEAND 480
+VRFNYF N VDLERCVNGTRKIG++LRS+++E FKF DWFGR++FR+VGPA P E D
Sbjct: 421 VVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDED 480
Query: 481 GL-MSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQATL 540
GL + +FCR+SVSTIWHYHGGCV GKVVD NYRVIGMEALRVVDGS F VSPGTNPQAT+
Sbjct: 481 GLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATV 540
Query: 541 MMLGRYVGLQIIKERS 556
MMLGR+VGLQII +RS
Sbjct: 541 MMLGRHVGLQIINQRS 546
BLAST of Sgr025368 vs. NCBI nr
Match:
XP_008462229.1 (PREDICTED: (R)-mandelonitrile lyase-like [Cucumis melo])
HSP 1 Score: 912.9 bits (2358), Expect = 1.3e-261
Identity = 452/556 (81.29%), Postives = 491/556 (88.31%), Query Frame = 0
Query: 1 MARRNFFYSQCLAFLVVFYGAISISANPKSLQQDLVTEPSYLKFVSNATAFPVEDYYDYI 60
MA R FF+SQ L FL++F + SA P S Q+D P YLKFVSNAT FP DYYDYI
Sbjct: 19 MASRIFFHSQILTFLLIFSAS---SALPNSSQKD----PDYLKFVSNATEFPKVDYYDYI 78
Query: 61 IVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSPVQAF 120
IVGGGTAGCPLAATLS+ FRVLVLERGG PHGNSNLMSQEGFLTTLMDDVH+R SPVQAF
Sbjct: 79 IVGGGTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDDVHSRYSPVQAF 138
Query: 121 TSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEIVFRP 180
TSEEGVPNARGRILGGSSAINAGFYSRADQ FF+NS + WD+ IVNQSYEWVEKEIVF+P
Sbjct: 139 TSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKP 198
Query: 181 NIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYATPFNI 240
N+KNWQSAVRD MVEAGI PYNGFSLDHV+GTKIGGSTFD++GRR SAADLL+YATPFNI
Sbjct: 199 NLKNWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAADLLSYATPFNI 258
Query: 241 KVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAMLRARGEVLVSAGAIGSPQ 300
KVA+YATVERILL ST + AIGVLYRDQ YHHAML ARGEV +SAGAIGSPQ
Sbjct: 259 KVAIYATVERILLGSTVK---RKPKAIGVLYRDQIGKYHHAMLHARGEVFLSAGAIGSPQ 318
Query: 301 LLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES 360
LLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPR GISILAPMPLEQSLIQVAGITES
Sbjct: 319 LLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRTGISILAPMPLEQSLIQVAGITES 378
Query: 361 GAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEKIIGPLSSGSMRLASTDIRVNP 420
G YLEAASNVIPFT+PPRSFFIRTPS P+YLTVATLMEKI GP++SG+MRLASTD+RVNP
Sbjct: 379 GTYLEAASNVIPFTTPPRSFFIRTPSPPVYLTVATLMEKITGPVASGAMRLASTDVRVNP 438
Query: 421 IVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFVGPALPVDEAND 480
+VRFNYF N VDLERCVNGTRKIGD+LRS+++E FKF DWFGR++FRFVGPA P D D
Sbjct: 439 VVRFNYFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPAYPEDGDED 498
Query: 481 GL-MSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQATL 540
GL + +FCR+SVSTIWHYHGGCV GKVVD NYRV+GMEALRVVDGS F VSPGTNPQAT+
Sbjct: 499 GLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSPGTNPQATV 558
Query: 541 MMLGRYVGLQIIKERS 556
MMLGR+VGLQII +RS
Sbjct: 559 MMLGRHVGLQIINQRS 564
BLAST of Sgr025368 vs. NCBI nr
Match:
KAA0059350.1 ((R)-mandelonitrile lyase-like [Cucumis melo var. makuwa] >TYK03976.1 (R)-mandelonitrile lyase-like [Cucumis melo var. makuwa])
HSP 1 Score: 912.9 bits (2358), Expect = 1.3e-261
Identity = 452/556 (81.29%), Postives = 491/556 (88.31%), Query Frame = 0
Query: 1 MARRNFFYSQCLAFLVVFYGAISISANPKSLQQDLVTEPSYLKFVSNATAFPVEDYYDYI 60
MA R FF+SQ L FL++F + SA P S Q+D P YLKFVSNAT FP DYYDYI
Sbjct: 19 MASRIFFHSQILTFLLIFSAS---SALPNSSQKD----PDYLKFVSNATEFPKVDYYDYI 78
Query: 61 IVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSPVQAF 120
IVGGGTAGCPLAATLS+ FRVLVLERGG PHGNSNLMSQEGFLTTLMDDVH+R SPVQAF
Sbjct: 79 IVGGGTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDDVHSRYSPVQAF 138
Query: 121 TSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEIVFRP 180
TSEEGVPNARGRILGGSSAINAGFYSRADQ FF+NS + WD+ IVNQSYEWVEKEIVF+P
Sbjct: 139 TSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKP 198
Query: 181 NIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYATPFNI 240
N+KNWQSAVRD MVEAGI PYNGFSLDHV+GTKIGGSTFD++GRR SAADLL+YATPFNI
Sbjct: 199 NLKNWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAADLLSYATPFNI 258
Query: 241 KVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAMLRARGEVLVSAGAIGSPQ 300
KVA+YATVERILL ST + AIGVLYRDQ YHHAML ARGEV +SAGAIGSPQ
Sbjct: 259 KVAIYATVERILLGSTVK---RKPKAIGVLYRDQIGKYHHAMLHARGEVFLSAGAIGSPQ 318
Query: 301 LLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES 360
LLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPR GISILAPMPLEQSLIQVAGITES
Sbjct: 319 LLLLSGIGSRPYLSSWGIPVVHHLPYVGHYLYDNPRTGISILAPMPLEQSLIQVAGITES 378
Query: 361 GAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEKIIGPLSSGSMRLASTDIRVNP 420
G YLEAASNVIPFT+PPRSFFIRTPS P+YLTVATLMEKI GP++SG+MRLASTD+RVNP
Sbjct: 379 GTYLEAASNVIPFTTPPRSFFIRTPSPPVYLTVATLMEKITGPVASGAMRLASTDVRVNP 438
Query: 421 IVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFVGPALPVDEAND 480
+VRFNYF N VDLERCVNGTRKIGD+LRS+++E FKF DWFGR++FRFVGPA P D D
Sbjct: 439 VVRFNYFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPAYPEDGDED 498
Query: 481 GL-MSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQATL 540
GL + +FCR+SVSTIWHYHGGCV GKVVD NYRV+GMEALRVVDGS F VSPGTNPQAT+
Sbjct: 499 GLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSPGTNPQATV 558
Query: 541 MMLGRYVGLQIIKERS 556
MMLGR+VGLQII +RS
Sbjct: 559 MMLGRHVGLQIINQRS 564
BLAST of Sgr025368 vs. NCBI nr
Match:
XP_022992218.1 ((R)-mandelonitrile lyase-like [Cucurbita maxima])
HSP 1 Score: 905.2 bits (2338), Expect = 2.8e-259
Identity = 452/557 (81.15%), Postives = 494/557 (88.69%), Query Frame = 0
Query: 1 MARRNFFYSQCLAFLVVFYGA-ISISANPKSLQQDLVTEPSYLKFVSNATAFPVEDYYDY 60
M R F YSQ LAFL++FY + SISA+ S Q+ EPSYLKFVS+A FP EDYYDY
Sbjct: 18 MPPRIFSYSQILAFLLLFYASPASISADLSSSQR----EPSYLKFVSDAADFPKEDYYDY 77
Query: 61 IIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSPVQA 120
IIVGGGTAGCPLAATLSS FRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVH+RDSPVQA
Sbjct: 78 IIVGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRDSPVQA 137
Query: 121 FTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEIVFR 180
FTSEEG+PNARGRILGGSSAINAGFYSRAD FF+NS + WD+ +VNQSYEWVEKEIVFR
Sbjct: 138 FTSEEGIPNARGRILGGSSAINAGFYSRADPAFFQNSHLYWDLAMVNQSYEWVEKEIVFR 197
Query: 181 PNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYATPFN 240
PN+KNWQSAVRD MVEAGI PY+GFS DH++GTKIGGSTFD++GRR SAADLLAYA P N
Sbjct: 198 PNLKNWQSAVRDGMVEAGIKPYHGFSFDHLIGTKIGGSTFDELGRRHSAADLLAYAKPCN 257
Query: 241 IKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAMLRARGEVLVSAGAIGSP 300
IKVAV+ATVERIL+A S+ +A GVLY DQ YHHA+LRARGEVLV+AGAIGSP
Sbjct: 258 IKVAVHATVERILVA-------SKATATGVLYHDQRGAYHHALLRARGEVLVAAGAIGSP 317
Query: 301 QLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITE 360
QLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISIL+PMPLEQSLIQVAGITE
Sbjct: 318 QLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILSPMPLEQSLIQVAGITE 377
Query: 361 SGAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEKIIGPLSSGSMRLASTDIRVN 420
SGAYLEA SNVIPFTSPP+SFFIR SAP+YLTVATLMEKIIGP+S GSMRLASTD+RVN
Sbjct: 378 SGAYLEATSNVIPFTSPPKSFFIRAQSAPVYLTVATLMEKIIGPVSFGSMRLASTDVRVN 437
Query: 421 PIVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFVGPALP-VDEA 480
P+V FNYF N VDLERCVNGTRKIG+VLRSRS+E FKFS W +R+FRFVGP P +E
Sbjct: 438 PVVWFNYFGNAVDLERCVNGTRKIGEVLRSRSMEGFKFSGWLWKREFRFVGPVYPEKEEK 497
Query: 481 NDGLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQAT 540
+DG + +FC+R+VSTIWHYHGGCV GKVVDRN+RVIGMEALRVVDGSTF VSPGTNPQAT
Sbjct: 498 DDGFLREFCKRTVSTIWHYHGGCVAGKVVDRNHRVIGMEALRVVDGSTFGVSPGTNPQAT 557
Query: 541 LMMLGRYVGLQIIKERS 556
LMMLGR+VGLQIIKERS
Sbjct: 558 LMMLGRHVGLQIIKERS 563
BLAST of Sgr025368 vs. ExPASy Swiss-Prot
Match:
Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)
HSP 1 Score: 760.8 bits (1963), Expect = 1.1e-218
Identity = 358/516 (69.38%), Postives = 440/516 (85.27%), Query Frame = 0
Query: 39 PSYLKFVSNATAFPVEDYYDYIIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMS 98
P +++F+SNAT F EDYYDYIIVGGGTAGCPLAATLS +FRVL+LERGGVP+ N+MS
Sbjct: 36 PGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMS 95
Query: 99 QEGFLTTLMDDVHTRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDV 158
+GFLTTL DV+ DSP Q+F SEEGVPNARGR+LGGSSAINAGFYSRAD+ FF NS +
Sbjct: 96 HDGFLTTL-TDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGL 155
Query: 159 NWDIKIVNQSYEWVEKEIVFRPNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGST 218
WD+ VNQSYEWVE+ IVFRP ++ WQ+A+RDA++E G++P+NGF+L+H VGTKIGGST
Sbjct: 156 VWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGST 215
Query: 219 FDDMGRRRSAADLLAYATPFNIKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGY 278
FD GRR S+ADLL YA NI+VAVYATVER+LLAS+ + SGS SAIGV+YRDQ +
Sbjct: 216 FDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRF 275
Query: 279 HHAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNG 338
HHA++R RGEV++SAGA+GSPQLL LSGIG R YLS+WGIPV P+VG ++YDNPRNG
Sbjct: 276 HHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNG 335
Query: 339 ISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLME 398
ISI+ P+P+E SLIQV G+TE GA+LEAASNVIPF SP S FIR P++PLY+ V T+ME
Sbjct: 336 ISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIME 395
Query: 399 KIIGPLSSGSMRLASTDIRVNPIVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFS 458
KI+GP+S G +RLASTD+R+NP+VRFNYFS+P DLERCVNGTRKIG++LRSR++++F
Sbjct: 396 KILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIR 455
Query: 459 DWFGRRDFRFVGPALPVDEANDGLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEA 518
+WFG R FRFVG LPVD++ND +M+DFCRR+VSTIWHYHGG VVGKVVD + +VIG+ +
Sbjct: 456 EWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNS 515
Query: 519 LRVVDGSTFAVSPGTNPQATLMMLGRYVGLQIIKER 555
LR+VDGSTF +SPGTNPQATLMMLGRY+GL++++ER
Sbjct: 516 LRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550
BLAST of Sgr025368 vs. ExPASy Swiss-Prot
Match:
P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)
HSP 1 Score: 516.2 bits (1328), Expect = 4.8e-145
Identity = 255/517 (49.32%), Postives = 359/517 (69.44%), Query Frame = 0
Query: 40 SYLKFVSNATAFPVEDYYDYIIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQ 99
SYL+F +AT +E YDY+IVGGGT+GCPLAATLS ++VLVLERG +P N+++
Sbjct: 37 SYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTA 96
Query: 100 EGFLTTLMDDVHTRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVN 159
+GF+ L + + +PV+ F SE+G+ N RGR+LGG+S INAG Y+RA+ + S V+
Sbjct: 97 DGFVYNLQQEDDGK-TPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASGVD 156
Query: 160 WDIKIVNQSYEWVEKEIVFRPNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTF 219
WD+ +VN++YEWVE IVF+PN + WQS A +EAG++P +GFSLDH GT+I GSTF
Sbjct: 157 WDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTF 216
Query: 220 DDMGRRRSAADLLAYATPFNIKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYH 279
D+ G R +A +LL N++V V+A+VE+I+ ++ +A GV+YRD H
Sbjct: 217 DNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGL-----TATGVIYRDSNGTPH 276
Query: 280 HAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGI 339
A +R++GEV+VSAG IG+PQLLLLSG+G YLSS IPV PYVG +L+DNPRN I
Sbjct: 277 RAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFI 336
Query: 340 SILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEK 399
+IL P P+E +++ V GI S + + + + +PFT+PP SFF T T A K
Sbjct: 337 NILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPFTTPPFSFFPSTSYPLPNSTFAHFASK 396
Query: 400 IIGPLSSGSMRL-ASTDIRVNPIVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFS 459
+ GPLS GS+ L +S+++RV+P V+FNY+SNP DL CV+G +KIG++L + +L+ +K
Sbjct: 397 VAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVE 456
Query: 460 DWFGRRDFRFVGPALPVDEANDGLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEA 519
D G F +G LP D+ +D FCR SV++ WHYHGGC+VGKV+D ++RV G++A
Sbjct: 457 DLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDA 516
Query: 520 LRVVDGSTFAVSPGTNPQATLMMLGRYVGLQIIKERS 556
LRVVDGSTF +P ++PQ +MLGRYVG++I++ERS
Sbjct: 517 LRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERS 545
BLAST of Sgr025368 vs. ExPASy Swiss-Prot
Match:
P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)
HSP 1 Score: 513.8 bits (1322), Expect = 2.4e-144
Identity = 260/517 (50.29%), Postives = 353/517 (68.28%), Query Frame = 0
Query: 40 SYLKFVSNATAFPVEDYYDYIIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQ 99
SYL FV +AT +E YDYIIVGGGTAGCPLAATLS+N+ VLVLERG +P NL+
Sbjct: 37 SYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLIS 96
Query: 100 EGFLTTLMDDVHTRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVN 159
+GF+ L + + +PV+ F SE+G+ N RGR+LGG+S INAG Y RA+ FF + +
Sbjct: 97 DGFVYNLQQEDDGK-TPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIE 156
Query: 160 WDIKIVNQSYEWVEKEIVFRPNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTF 219
WD+ +VNQ+YEWVE IVF P+ + WQ+ + A +EAGI P NGFS+DH+ GT++ GSTF
Sbjct: 157 WDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTF 216
Query: 220 DDMGRRRSAADLLAYATPFNIKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYH 279
D+ G R ++ +LL P N++VAV A VE+I+ +S + S +AIGV+Y D H
Sbjct: 217 DNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNT----SGVTAIGVIYTDSNGTTH 276
Query: 280 HAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGI 339
A +R GEV++SAG IGSPQLLLLSG+G YL+S I V PYVG Y+YDNPRN I
Sbjct: 277 QAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFI 336
Query: 340 SILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEK 399
+IL P P+E S + V GIT + + + + +PF +PP SFF T T A ++ K
Sbjct: 337 NILPPNPIEASTVTVLGITSD--FYQCSISSLPFDTPPFSFFPTTSYPLPNQTFAHIVNK 396
Query: 400 IIGPLSSGSMRL-ASTDIRVNPIVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFS 459
+ GPLS G++ L +S+D+RV P V+FNY+SN DL CV+G +K+G+VL + +LE +K
Sbjct: 397 VPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVE 456
Query: 460 DWFGRRDFRFVGPALPVDEANDGLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEA 519
D G F +G LP ++ +D FCR SV++ WHYHGGC+VGKV+D +RV G+ A
Sbjct: 457 DLPGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINA 516
Query: 520 LRVVDGSTFAVSPGTNPQATLMMLGRYVGLQIIKERS 556
LRVVDGSTF +P ++PQ +MLGRY+G+QI++ERS
Sbjct: 517 LRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQERS 546
BLAST of Sgr025368 vs. ExPASy Swiss-Prot
Match:
Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)
HSP 1 Score: 510.8 bits (1314), Expect = 2.0e-143
Identity = 256/520 (49.23%), Postives = 360/520 (69.23%), Query Frame = 0
Query: 40 SYLKFVSNATAFPVEDYYDYIIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQ 99
SYL F +AT +E YDY+IVGGGT+GCPLAATLS ++VLVLERG +P N+++
Sbjct: 37 SYLSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTA 96
Query: 100 EGFLTTLMDDVHTRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVN 159
+GF+ L + + +PV+ F SE+G+ N RGR+LGG+S INAG Y+RA+ + S V+
Sbjct: 97 DGFVYNLQQEDDGK-TPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGVD 156
Query: 160 WDIKIVNQSYEWVEKEIVFRPNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTF 219
WD+ +VNQ+YEWVE IV++PN ++WQS + A +EAG++P +GFSLDH GT+I GSTF
Sbjct: 157 WDMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTF 216
Query: 220 DDMGRRRSAADLLAYATPFNIKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYH 279
D+ G R +A +LL N++V V+A+VE+I+ ++ +A GV+YRD H
Sbjct: 217 DNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGL-----TATGVIYRDSNGTPH 276
Query: 280 HAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGI 339
A +R++GEV+VSAG IG+PQLLLLSG+G YLSS IPV PYVG +L+DNPRN I
Sbjct: 277 QAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFI 336
Query: 340 SILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFFIRTPSAPLYL---TVATL 399
+IL P P+E +++ V GI S + + + + +PFT+PP FF PSA L T A
Sbjct: 337 NILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPFTTPPFGFF---PSASYPLPNSTFAHF 396
Query: 400 MEKIIGPLSSGSMRL-ASTDIRVNPIVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENF 459
K+ GPLS GS+ L +S+++RV+P V+FNY+SN DL CV+G +KIG++L + +L+ +
Sbjct: 397 ASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPY 456
Query: 460 KFSDWFGRRDFRFVGPALPVDEANDGLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIG 519
K D G F +G LP D+ +D FCR SV++ WHYHGGC+VGKV+D ++RV G
Sbjct: 457 KVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTG 516
Query: 520 MEALRVVDGSTFAVSPGTNPQATLMMLGRYVGLQIIKERS 556
+ ALRVVDGSTF +P ++PQ +MLGRYVG++I++ERS
Sbjct: 517 INALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERS 545
BLAST of Sgr025368 vs. ExPASy Swiss-Prot
Match:
O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)
HSP 1 Score: 498.0 bits (1281), Expect = 1.3e-139
Identity = 253/519 (48.75%), Postives = 351/519 (67.63%), Query Frame = 0
Query: 40 SYLKFVSNATAFPVEDYYDYIIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQ 99
SYLKFV NAT +E YDYI++GGGT+GCPLAATLS ++VL+LERG + N ++
Sbjct: 37 SYLKFVYNATDTSLEGSYDYIVIGGGTSGCPLAATLSEKYKVLLLERGTIATEYPNTLTA 96
Query: 100 EGFLTTL--MDDVHTRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSD 159
+GF L DD T PV+ F SE+G+ N R RILGG++ INAG Y+RA+ F+ +
Sbjct: 97 DGFAYNLQQQDDGKT---PVERFVSEDGIDNVRARILGGTTIINAGVYARANISFYSQTG 156
Query: 160 VNWDIKIVNQSYEWVEKEIVFRPNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGS 219
+ WD+ +VN++YEWVE IV +PN ++WQS + + +EAGI P NGFSLDH GT++ GS
Sbjct: 157 IEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGS 216
Query: 220 TFDDMGRRRSAADLLAYATPFNIKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADG 279
TFD+ G R +A +LL P N+ VAV A+VE+IL +S + S SAIGV+Y D
Sbjct: 217 TFDNNGTRHAADELLNKGDPNNLLVAVQASVEKILFSSNT----SNLSAIGVIYTDSDGN 276
Query: 280 YHHAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRN 339
H A +R GEV+VSAG IG+PQLLLLSG+G YLSS I V PYVG +LY+NPRN
Sbjct: 277 SHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITVVQPNPYVGQFLYNNPRN 336
Query: 340 GISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLM 399
I+ P P+E S++ V GI Y + + + +PF++PP S F T T A ++
Sbjct: 337 FINNFPPNPIEASVVTVLGIRSD--YYQVSLSSLPFSTPPFSLFPTTSYPLPNSTFAHIV 396
Query: 400 EKIIGPLSSGSMRL-ASTDIRVNPIVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFK 459
++ GPLS GS+ L +S+D+R+ P ++FNY+SN DL CV+G +K+GD+LR+++LE +K
Sbjct: 397 SQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSNSTDLANCVSGMKKLGDLLRTKALEPYK 456
Query: 460 FSDWFGRRDFRFVGPALPVDEANDGLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGM 519
D G F ++G LP ++ +D FC +V++ WHYHGG +VGKV+D ++RV+G+
Sbjct: 457 ARDVLGIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHYHGGSLVGKVLDDSFRVMGI 516
Query: 520 EALRVVDGSTFAVSPGTNPQATLMMLGRYVGLQIIKERS 556
+ALRVVD STF P ++PQ +MLGRYVGLQI++ERS
Sbjct: 517 KALRVVDASTFPYEPNSHPQGFYLMLGRYVGLQILQERS 546
BLAST of Sgr025368 vs. ExPASy TrEMBL
Match:
A0A0A0KCB1 (GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447930 PE=3 SV=1)
HSP 1 Score: 914.1 bits (2361), Expect = 2.9e-262
Identity = 455/556 (81.83%), Postives = 492/556 (88.49%), Query Frame = 0
Query: 1 MARRNFFYSQCLAFLVVFYGAISISANPKSLQQDLVTEPSYLKFVSNATAFPVEDYYDYI 60
M R F+SQ L FL++F + SA P S QQD P YLKFVSNAT FP DYYDYI
Sbjct: 1 MESRISFHSQILTFLLIFSAS---SALPNSSQQD----PDYLKFVSNATEFPEVDYYDYI 60
Query: 61 IVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSPVQAF 120
IVGGGTAGCPLAATLSS FRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVH+R+SPVQAF
Sbjct: 61 IVGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAF 120
Query: 121 TSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEIVFRP 180
TSEEGVPNARGRILGGSSAINAGFYSRADQ FF+NS + WD+ IVNQSYEWVEKEIVF+P
Sbjct: 121 TSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKP 180
Query: 181 NIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYATPFNI 240
N+KNWQSAVRD MVEAGI PYNGFSLDHV+GTKIGGSTFD++GRR SAADLL++ATPFNI
Sbjct: 181 NLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSHATPFNI 240
Query: 241 KVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAMLRARGEVLVSAGAIGSPQ 300
KVA+YA VERILLAST R AIGVLYRDQ YHHAML ARGEV +SAGAIGSPQ
Sbjct: 241 KVAIYANVERILLASTVK---RRPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQ 300
Query: 301 LLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES 360
LLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES
Sbjct: 301 LLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES 360
Query: 361 GAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEKIIGPLSSGSMRLASTDIRVNP 420
G YLEAASNVIPFTSPPRSFFIRTPS P+YLTVATLMEKI GP++SGSMRLASTD+RVNP
Sbjct: 361 GTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNP 420
Query: 421 IVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFVGPALPVDEAND 480
+VRFNYF N VDLERCVNGTRKIG++LRS+++E FKF DWFGR++FR+VGPA P E D
Sbjct: 421 VVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDED 480
Query: 481 GL-MSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQATL 540
GL + +FCR+SVSTIWHYHGGCV GKVVD NYRVIGMEALRVVDGS F VSPGTNPQAT+
Sbjct: 481 GLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATV 540
Query: 541 MMLGRYVGLQIIKERS 556
MMLGR+VGLQII +RS
Sbjct: 541 MMLGRHVGLQIINQRS 546
BLAST of Sgr025368 vs. ExPASy TrEMBL
Match:
A0A5D3C0K1 ((R)-mandelonitrile lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001740 PE=3 SV=1)
HSP 1 Score: 912.9 bits (2358), Expect = 6.5e-262
Identity = 452/556 (81.29%), Postives = 491/556 (88.31%), Query Frame = 0
Query: 1 MARRNFFYSQCLAFLVVFYGAISISANPKSLQQDLVTEPSYLKFVSNATAFPVEDYYDYI 60
MA R FF+SQ L FL++F + SA P S Q+D P YLKFVSNAT FP DYYDYI
Sbjct: 19 MASRIFFHSQILTFLLIFSAS---SALPNSSQKD----PDYLKFVSNATEFPKVDYYDYI 78
Query: 61 IVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSPVQAF 120
IVGGGTAGCPLAATLS+ FRVLVLERGG PHGNSNLMSQEGFLTTLMDDVH+R SPVQAF
Sbjct: 79 IVGGGTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDDVHSRYSPVQAF 138
Query: 121 TSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEIVFRP 180
TSEEGVPNARGRILGGSSAINAGFYSRADQ FF+NS + WD+ IVNQSYEWVEKEIVF+P
Sbjct: 139 TSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKP 198
Query: 181 NIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYATPFNI 240
N+KNWQSAVRD MVEAGI PYNGFSLDHV+GTKIGGSTFD++GRR SAADLL+YATPFNI
Sbjct: 199 NLKNWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAADLLSYATPFNI 258
Query: 241 KVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAMLRARGEVLVSAGAIGSPQ 300
KVA+YATVERILL ST + AIGVLYRDQ YHHAML ARGEV +SAGAIGSPQ
Sbjct: 259 KVAIYATVERILLGSTVK---RKPKAIGVLYRDQIGKYHHAMLHARGEVFLSAGAIGSPQ 318
Query: 301 LLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES 360
LLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPR GISILAPMPLEQSLIQVAGITES
Sbjct: 319 LLLLSGIGSRPYLSSWGIPVVHHLPYVGHYLYDNPRTGISILAPMPLEQSLIQVAGITES 378
Query: 361 GAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEKIIGPLSSGSMRLASTDIRVNP 420
G YLEAASNVIPFT+PPRSFFIRTPS P+YLTVATLMEKI GP++SG+MRLASTD+RVNP
Sbjct: 379 GTYLEAASNVIPFTTPPRSFFIRTPSPPVYLTVATLMEKITGPVASGAMRLASTDVRVNP 438
Query: 421 IVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFVGPALPVDEAND 480
+VRFNYF N VDLERCVNGTRKIGD+LRS+++E FKF DWFGR++FRFVGPA P D D
Sbjct: 439 VVRFNYFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPAYPEDGDED 498
Query: 481 GL-MSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQATL 540
GL + +FCR+SVSTIWHYHGGCV GKVVD NYRV+GMEALRVVDGS F VSPGTNPQAT+
Sbjct: 499 GLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSPGTNPQATV 558
Query: 541 MMLGRYVGLQIIKERS 556
MMLGR+VGLQII +RS
Sbjct: 559 MMLGRHVGLQIINQRS 564
BLAST of Sgr025368 vs. ExPASy TrEMBL
Match:
A0A1S3CHZ4 ((R)-mandelonitrile lyase-like OS=Cucumis melo OX=3656 GN=LOC103500635 PE=3 SV=1)
HSP 1 Score: 912.9 bits (2358), Expect = 6.5e-262
Identity = 452/556 (81.29%), Postives = 491/556 (88.31%), Query Frame = 0
Query: 1 MARRNFFYSQCLAFLVVFYGAISISANPKSLQQDLVTEPSYLKFVSNATAFPVEDYYDYI 60
MA R FF+SQ L FL++F + SA P S Q+D P YLKFVSNAT FP DYYDYI
Sbjct: 19 MASRIFFHSQILTFLLIFSAS---SALPNSSQKD----PDYLKFVSNATEFPKVDYYDYI 78
Query: 61 IVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSPVQAF 120
IVGGGTAGCPLAATLS+ FRVLVLERGG PHGNSNLMSQEGFLTTLMDDVH+R SPVQAF
Sbjct: 79 IVGGGTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDDVHSRYSPVQAF 138
Query: 121 TSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEIVFRP 180
TSEEGVPNARGRILGGSSAINAGFYSRADQ FF+NS + WD+ IVNQSYEWVEKEIVF+P
Sbjct: 139 TSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKP 198
Query: 181 NIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYATPFNI 240
N+KNWQSAVRD MVEAGI PYNGFSLDHV+GTKIGGSTFD++GRR SAADLL+YATPFNI
Sbjct: 199 NLKNWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAADLLSYATPFNI 258
Query: 241 KVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAMLRARGEVLVSAGAIGSPQ 300
KVA+YATVERILL ST + AIGVLYRDQ YHHAML ARGEV +SAGAIGSPQ
Sbjct: 259 KVAIYATVERILLGSTVK---RKPKAIGVLYRDQIGKYHHAMLHARGEVFLSAGAIGSPQ 318
Query: 301 LLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITES 360
LLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPR GISILAPMPLEQSLIQVAGITES
Sbjct: 319 LLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRTGISILAPMPLEQSLIQVAGITES 378
Query: 361 GAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEKIIGPLSSGSMRLASTDIRVNP 420
G YLEAASNVIPFT+PPRSFFIRTPS P+YLTVATLMEKI GP++SG+MRLASTD+RVNP
Sbjct: 379 GTYLEAASNVIPFTTPPRSFFIRTPSPPVYLTVATLMEKITGPVASGAMRLASTDVRVNP 438
Query: 421 IVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFVGPALPVDEAND 480
+VRFNYF N VDLERCVNGTRKIGD+LRS+++E FKF DWFGR++FRFVGPA P D D
Sbjct: 439 VVRFNYFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPAYPEDGDED 498
Query: 481 GL-MSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQATL 540
GL + +FCR+SVSTIWHYHGGCV GKVVD NYRV+GMEALRVVDGS F VSPGTNPQAT+
Sbjct: 499 GLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSPGTNPQATV 558
Query: 541 MMLGRYVGLQIIKERS 556
MMLGR+VGLQII +RS
Sbjct: 559 MMLGRHVGLQIINQRS 564
BLAST of Sgr025368 vs. ExPASy TrEMBL
Match:
A0A6J1JYK6 ((R)-mandelonitrile lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111488606 PE=3 SV=1)
HSP 1 Score: 905.2 bits (2338), Expect = 1.3e-259
Identity = 452/557 (81.15%), Postives = 494/557 (88.69%), Query Frame = 0
Query: 1 MARRNFFYSQCLAFLVVFYGA-ISISANPKSLQQDLVTEPSYLKFVSNATAFPVEDYYDY 60
M R F YSQ LAFL++FY + SISA+ S Q+ EPSYLKFVS+A FP EDYYDY
Sbjct: 18 MPPRIFSYSQILAFLLLFYASPASISADLSSSQR----EPSYLKFVSDAADFPKEDYYDY 77
Query: 61 IIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSPVQA 120
IIVGGGTAGCPLAATLSS FRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVH+RDSPVQA
Sbjct: 78 IIVGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRDSPVQA 137
Query: 121 FTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEIVFR 180
FTSEEG+PNARGRILGGSSAINAGFYSRAD FF+NS + WD+ +VNQSYEWVEKEIVFR
Sbjct: 138 FTSEEGIPNARGRILGGSSAINAGFYSRADPAFFQNSHLYWDLAMVNQSYEWVEKEIVFR 197
Query: 181 PNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYATPFN 240
PN+KNWQSAVRD MVEAGI PY+GFS DH++GTKIGGSTFD++GRR SAADLLAYA P N
Sbjct: 198 PNLKNWQSAVRDGMVEAGIKPYHGFSFDHLIGTKIGGSTFDELGRRHSAADLLAYAKPCN 257
Query: 241 IKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAMLRARGEVLVSAGAIGSP 300
IKVAV+ATVERIL+A S+ +A GVLY DQ YHHA+LRARGEVLV+AGAIGSP
Sbjct: 258 IKVAVHATVERILVA-------SKATATGVLYHDQRGAYHHALLRARGEVLVAAGAIGSP 317
Query: 301 QLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITE 360
QLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISIL+PMPLEQSLIQVAGITE
Sbjct: 318 QLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILSPMPLEQSLIQVAGITE 377
Query: 361 SGAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEKIIGPLSSGSMRLASTDIRVN 420
SGAYLEA SNVIPFTSPP+SFFIR SAP+YLTVATLMEKIIGP+S GSMRLASTD+RVN
Sbjct: 378 SGAYLEATSNVIPFTSPPKSFFIRAQSAPVYLTVATLMEKIIGPVSFGSMRLASTDVRVN 437
Query: 421 PIVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFVGPALP-VDEA 480
P+V FNYF N VDLERCVNGTRKIG+VLRSRS+E FKFS W +R+FRFVGP P +E
Sbjct: 438 PVVWFNYFGNAVDLERCVNGTRKIGEVLRSRSMEGFKFSGWLWKREFRFVGPVYPEKEEK 497
Query: 481 NDGLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQAT 540
+DG + +FC+R+VSTIWHYHGGCV GKVVDRN+RVIGMEALRVVDGSTF VSPGTNPQAT
Sbjct: 498 DDGFLREFCKRTVSTIWHYHGGCVAGKVVDRNHRVIGMEALRVVDGSTFGVSPGTNPQAT 557
Query: 541 LMMLGRYVGLQIIKERS 556
LMMLGR+VGLQIIKERS
Sbjct: 558 LMMLGRHVGLQIIKERS 563
BLAST of Sgr025368 vs. ExPASy TrEMBL
Match:
A0A6J1GR64 ((R)-mandelonitrile lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111456379 PE=3 SV=1)
HSP 1 Score: 902.5 bits (2331), Expect = 8.7e-259
Identity = 451/558 (80.82%), Postives = 494/558 (88.53%), Query Frame = 0
Query: 1 MARRNFFYSQCLAFLVVFYGA-ISISANPKSLQQDLVTEPSYLKFVSNATAFPVEDYYDY 60
M R F YSQ LAFL++FY + SISA+ S Q+ EPSYLKFVS+A FP EDYYDY
Sbjct: 18 MPPRIFSYSQILAFLLLFYASPASISADLSSSQR----EPSYLKFVSDAADFPKEDYYDY 77
Query: 61 IIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSPVQA 120
IIVGGGTAGCPLAATLSS FRVLVLERGGVPH NSNLMSQEGFLTTLMDDVH+RDSPVQA
Sbjct: 78 IIVGGGTAGCPLAATLSSKFRVLVLERGGVPHANSNLMSQEGFLTTLMDDVHSRDSPVQA 137
Query: 121 FTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEIVFR 180
FTSEEG+PNARGRILGGSSAINAGFYSRAD FF+NS ++WD+ +VNQSYEWVEKEIVFR
Sbjct: 138 FTSEEGIPNARGRILGGSSAINAGFYSRADPAFFQNSRLHWDLAMVNQSYEWVEKEIVFR 197
Query: 181 PNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYATPFN 240
PN+KNWQSAVRD MVEAGI PY+GFS DH++GTKIGGSTFD++GRR SAADLLAYA P N
Sbjct: 198 PNLKNWQSAVRDGMVEAGIKPYHGFSFDHLIGTKIGGSTFDELGRRHSAADLLAYAKPCN 257
Query: 241 IKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAMLRARGEVLVSAGAIGSP 300
IKVAV+ATVERIL+AS +A A GVLY DQ YHHA+LRARGEVLV+AGAIGSP
Sbjct: 258 IKVAVHATVERILVASKAA-------ATGVLYHDQRGAYHHALLRARGEVLVAAGAIGSP 317
Query: 301 QLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITE 360
LLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISIL+PMPLEQSLIQVAGITE
Sbjct: 318 HLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILSPMPLEQSLIQVAGITE 377
Query: 361 SGAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLMEKIIGPLSSGSMRLASTDIRVN 420
SGAYLEA SNVIPFTSPP+SFFIR SAP+YLTVATLMEKIIGP+S GSMRLASTD+RVN
Sbjct: 378 SGAYLEATSNVIPFTSPPKSFFIRAQSAPVYLTVATLMEKIIGPVSFGSMRLASTDVRVN 437
Query: 421 PIVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFVGPALP-VDEA 480
P+V FNYF N VDLERCVNGTRKIG+VLRSRS+E FKFS W +R+FRFVGP P +E
Sbjct: 438 PVVWFNYFGNAVDLERCVNGTRKIGEVLRSRSMEGFKFSGWLWKREFRFVGPVYPEKEEK 497
Query: 481 NDGLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQAT 540
+DG + +FC+R+VSTIWHYHGGCV GKVVDRN+RVIGMEALRVVDGSTF VSPGTNPQAT
Sbjct: 498 DDGFLREFCKRTVSTIWHYHGGCVAGKVVDRNHRVIGMEALRVVDGSTFGVSPGTNPQAT 557
Query: 541 LMMLGRYVGLQIIKERST 557
LMMLGR+VGLQIIKERS+
Sbjct: 558 LMMLGRHVGLQIIKERSS 564
BLAST of Sgr025368 vs. TAIR 10
Match:
AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )
HSP 1 Score: 760.8 bits (1963), Expect = 7.9e-220
Identity = 358/516 (69.38%), Postives = 440/516 (85.27%), Query Frame = 0
Query: 39 PSYLKFVSNATAFPVEDYYDYIIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMS 98
P +++F+SNAT F EDYYDYIIVGGGTAGCPLAATLS +FRVL+LERGGVP+ N+MS
Sbjct: 36 PGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMS 95
Query: 99 QEGFLTTLMDDVHTRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDV 158
+GFLTTL DV+ DSP Q+F SEEGVPNARGR+LGGSSAINAGFYSRAD+ FF NS +
Sbjct: 96 HDGFLTTL-TDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGL 155
Query: 159 NWDIKIVNQSYEWVEKEIVFRPNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGST 218
WD+ VNQSYEWVE+ IVFRP ++ WQ+A+RDA++E G++P+NGF+L+H VGTKIGGST
Sbjct: 156 VWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGST 215
Query: 219 FDDMGRRRSAADLLAYATPFNIKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGY 278
FD GRR S+ADLL YA NI+VAVYATVER+LLAS+ + SGS SAIGV+YRDQ +
Sbjct: 216 FDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRF 275
Query: 279 HHAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNG 338
HHA++R RGEV++SAGA+GSPQLL LSGIG R YLS+WGIPV P+VG ++YDNPRNG
Sbjct: 276 HHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNG 335
Query: 339 ISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFFIRTPSAPLYLTVATLME 398
ISI+ P+P+E SLIQV G+TE GA+LEAASNVIPF SP S FIR P++PLY+ V T+ME
Sbjct: 336 ISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIME 395
Query: 399 KIIGPLSSGSMRLASTDIRVNPIVRFNYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFS 458
KI+GP+S G +RLASTD+R+NP+VRFNYFS+P DLERCVNGTRKIG++LRSR++++F
Sbjct: 396 KILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIR 455
Query: 459 DWFGRRDFRFVGPALPVDEANDGLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEA 518
+WFG R FRFVG LPVD++ND +M+DFCRR+VSTIWHYHGG VVGKVVD + +VIG+ +
Sbjct: 456 EWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNS 515
Query: 519 LRVVDGSTFAVSPGTNPQATLMMLGRYVGLQIIKER 555
LR+VDGSTF +SPGTNPQATLMMLGRY+GL++++ER
Sbjct: 516 LRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550
BLAST of Sgr025368 vs. TAIR 10
Match:
AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )
HSP 1 Score: 458.0 bits (1177), Expect = 1.1e-128
Identity = 252/558 (45.16%), Postives = 349/558 (62.54%), Query Frame = 0
Query: 39 PSYLKFVSNATAFPVEDYYDYIIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMS 98
P+Y F+ +AT P YYDYII+GGGTAGCPLAATLS N VL+LERG P+ N N+
Sbjct: 29 PNY-SFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNNPNITR 88
Query: 99 QEGFLTTLMDDVHTRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDV 158
F L D + SP Q F SE+GV NAR R+LGG SA+NAGFY+RA + RN +
Sbjct: 89 LSAFGAALSD--LSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYVRN--M 148
Query: 159 NWDIKIVNQSYEWVEKEIVFRPNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGST 218
WD + N+SY+WVE ++ F+P + WQ+AVRD ++EAGI P NGF+ DH+ GTK GG+
Sbjct: 149 GWDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTI 208
Query: 219 FDDMGRRRSAADLLAYATPFNIKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGY 278
FD G R +AADLL YA P I V ++ATV RIL + ++ A GV+YRD+
Sbjct: 209 FDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGT---TKPIANGVVYRDRTGQA 268
Query: 279 HHAMLR--ARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPR 338
H A L+ A E+++SAG +GSPQLL+LSG+G L + I V P+VG +YDNP
Sbjct: 269 HRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPM 328
Query: 339 NGISILAPMPLEQSLIQVAGITESGAYLEAASNV--------IPFTSPPRSFF-IRTPSA 398
N + + +P+P+E SLI+V GIT G Y+EAA +S R ++ + +P A
Sbjct: 329 NAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFSPRA 388
Query: 399 PLY-------LTVAT------LMEKIIGPLSSGSMRLASTDIRVNPIVRFNYFSNPVDLE 458
L L+ A L+EK++GPLS+G + L + + + NPIV FNYF +P DL+
Sbjct: 389 TLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDDLK 448
Query: 459 RCVNGTRKIGDVLRSRSLENFKFSDWFGRRDFRFV----------------GPALPVDEA 518
RCV G + I V++S++ +K++D F ++ GP + +
Sbjct: 449 RCVRGIQTIERVVQSKAFSRYKYAD----VSFEYLLNLTASTPVNLRPPRSGPGASLPPS 508
Query: 519 NDGLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQAT 557
+ +FC+ +V+TIWHYHGGCVVG+VVD +Y+VIG++ LRV+D ST PGTNPQAT
Sbjct: 509 AE----EFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQAT 568
BLAST of Sgr025368 vs. TAIR 10
Match:
AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )
HSP 1 Score: 451.1 bits (1159), Expect = 1.3e-126
Identity = 245/536 (45.71%), Postives = 335/536 (62.50%), Query Frame = 0
Query: 57 YDYIIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHTRDSP 116
YDYI++GGGTAGCPLAATLS NF VLVLERGGVP N+N+ F L D + S
Sbjct: 63 YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLAD--ISASSA 122
Query: 117 VQAFTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKIVNQSYEWVEKEI 176
QAF S +GV NAR R+LGG S INAGFYSRAD F + + WD K+V +SY WVE+EI
Sbjct: 123 SQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRA--GWDPKLVKESYPWVEREI 182
Query: 177 VFRPNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGRRRSAADLLAYAT 236
V +P + WQ A+RD+++E G+ P+NGF+ DHV GTKIGG+ FD GRR +AA+LLAYA
Sbjct: 183 VHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYAN 242
Query: 237 PFNIKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAML--RARGEVLVSAG 296
P ++V +YATV++I+ + SG+R GV+++D+ H A+L R EV++S+G
Sbjct: 243 PQKLRVLIYATVQKIVFDT----SGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSG 302
Query: 297 AIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQV 356
AIGSPQ+L+LSGIG + L IPV +VG + DNP N I + + P+EQSLIQ
Sbjct: 303 AIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQT 362
Query: 357 AGITESGAYLEAASNVIPFTSPPRS----FFIRTPSAPLYLTV----------------- 416
GIT+ G Y+EA++ F P S + I + L+ T+
Sbjct: 363 VGITKMGVYVEASTG---FGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRN 422
Query: 417 ----------ATLMEKIIGPLSSGSMRLASTDIRVNPIVRFNYFSNPVDLERCVNGTRKI 476
+ ++EK+ P+S G + L +T++ NP V FNYF +PVDL+RCV R +
Sbjct: 423 KYQLHEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLV 482
Query: 477 GDVLRSRSLENFKFSDWFGRRDFRFVGPALPVD----EAND-GLMSDFCRRSVSTIWHYH 536
V+ S N+ D + ++ + ND M+ FC+ +V TIWHYH
Sbjct: 483 SKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYH 542
Query: 537 GGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQATLMMLGRYVGLQIIKER 555
GGC+VGKVV N +V+G++ LRV+DGSTF SPGTNPQAT+MM+GRY+G++I++ER
Sbjct: 543 GGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587
BLAST of Sgr025368 vs. TAIR 10
Match:
AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )
HSP 1 Score: 439.9 bits (1130), Expect = 3.1e-123
Identity = 252/557 (45.24%), Postives = 335/557 (60.14%), Query Frame = 0
Query: 41 YLKFVSNATAFPVEDYYDYIIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQE 100
Y F+ +AT P+ +DYII+GGGT+GC LAATLS N VLVLERGG P+ N E
Sbjct: 30 YYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIE 89
Query: 101 GFLTTLMDDVHTRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNW 160
F TTL + + S Q F SE+GV N R R+LGG S +NAGFY+RA ++ + ++ W
Sbjct: 90 NFATTLSNT--SPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKETE--W 149
Query: 161 DIKIVNQSYEWVEKEIVFRPNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFD 220
V +YEWVEK++ F+P + WQ+A +D ++EAG PYNGF+ DH+ GTKIGG+ FD
Sbjct: 150 KTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFD 209
Query: 221 DMGRRRSAADLLAYATPFNIKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHH 280
G R +AADLL YA P NI V ++A+V +IL + R A GV+++D H
Sbjct: 210 RAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGR---PRPKAYGVIFQDANGVLHK 269
Query: 281 AML--RARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGI-PVTHHLPYVGHYLYDNPRN 340
A L + EV++SAGAIGSPQLL+LSGIG +L++ GI P+ P VG + DNP N
Sbjct: 270 AELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMN 329
Query: 341 GISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFF------------IRTP 400
I I +P P+E SLIQV GIT+ +Y+E AS VI S R FF RT
Sbjct: 330 AIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTT 389
Query: 401 S--APLYLT-------------------VATLMEKIIGPLSSGSMRLASTDIRVNPIVRF 460
S +P T +++KI GP+S G + L +T+ NP VRF
Sbjct: 390 STTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELRNTNPDDNPSVRF 449
Query: 461 NYFSNPVDLERCVNGTRKIGDVLRSRSLENFKFSD--WFGRRDFRFVGPA--LPVDEAND 520
NY+ P DL+ CV G I V+ S++ FK+ D G D P P +
Sbjct: 450 NYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSM 509
Query: 521 GLMSDFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQATLM 558
+ FC +V TIWHYHGGC VG+VVD+NYRV+G+++LRV+DGSTF SPGTNPQAT+M
Sbjct: 510 FNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVM 569
BLAST of Sgr025368 vs. TAIR 10
Match:
AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )
HSP 1 Score: 435.3 bits (1118), Expect = 7.6e-122
Identity = 250/553 (45.21%), Postives = 333/553 (60.22%), Query Frame = 0
Query: 45 VSNATAFPVEDYYDYIIVGGGTAGCPLAATLSSNFRVLVLERGGVPHGNSNLMSQEGFLT 104
+ +AT P+ +DYII+GGGT+GC LAATLS N VLVLERGG P+ N E F T
Sbjct: 1 MKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFAT 60
Query: 105 TLMDDVHTRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQDFFRNSDVNWDIKI 164
TL + + S Q F SE+GV N R R+LGG S +NAGFY+RA ++ + ++ W
Sbjct: 61 TLSNT--SPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKETE--WKTDE 120
Query: 165 VNQSYEWVEKEIVFRPNIKNWQSAVRDAMVEAGINPYNGFSLDHVVGTKIGGSTFDDMGR 224
V +YEWVEK++ F+P + WQ+A +D ++EAG PYNGF+ DH+ GTKIGG+ FD G
Sbjct: 121 VEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGH 180
Query: 225 RRSAADLLAYATPFNIKVAVYATVERILLASTSAYSGSRRSAIGVLYRDQADGYHHAML- 284
R +AADLL YA P NI V ++A+V +IL + R A GV+++D H A L
Sbjct: 181 RHTAADLLEYANPGNIVVYLHASVHKILFTTKGR---PRPKAYGVIFQDANGVLHKAELE 240
Query: 285 -RARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGI-PVTHHLPYVGHYLYDNPRNGISI 344
+ EV++SAGAIGSPQLL+LSGIG +L++ GI P+ P VG + DNP N I I
Sbjct: 241 KNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFI 300
Query: 345 LAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFF------------IRTPS--A 404
+P P+E SLIQV GIT+ +Y+E AS VI S R FF RT S +
Sbjct: 301 PSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTS 360
Query: 405 PLYLT-------------------VATLMEKIIGPLSSGSMRLASTDIRVNPIVRFNYFS 464
P T +++KI GP+S G + L +T+ NP VRFNY+
Sbjct: 361 PTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELRNTNPDDNPSVRFNYYQ 420
Query: 465 NPVDLERCVNGTRKIGDVLRSRSLENFKFSD--WFGRRDFRFVGPA--LPVDEANDGLMS 524
P DL+ CV G I V+ S++ FK+ D G D P P + +
Sbjct: 421 EPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMFNLR 480
Query: 525 DFCRRSVSTIWHYHGGCVVGKVVDRNYRVIGMEALRVVDGSTFAVSPGTNPQATLMMLGR 558
FC +V TIWHYHGGC VG+VVD+NYRV+G+++LRV+DGSTF SPGTNPQAT+MMLGR
Sbjct: 481 QFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLGR 540
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038899448.1 | 3.0e-269 | 83.99 | (R)-mandelonitrile lyase-like [Benincasa hispida] | [more] |
KGN45431.1 | 6.0e-262 | 81.83 | hypothetical protein Csa_016192 [Cucumis sativus] | [more] |
XP_008462229.1 | 1.3e-261 | 81.29 | PREDICTED: (R)-mandelonitrile lyase-like [Cucumis melo] | [more] |
KAA0059350.1 | 1.3e-261 | 81.29 | (R)-mandelonitrile lyase-like [Cucumis melo var. makuwa] >TYK03976.1 (R)-mandelo... | [more] |
XP_022992218.1 | 2.8e-259 | 81.15 | (R)-mandelonitrile lyase-like [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
Q9SSM2 | 1.1e-218 | 69.38 | (R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... | [more] |
P52706 | 4.8e-145 | 49.32 | (R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1 | [more] |
P52707 | 2.4e-144 | 50.29 | (R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1 | [more] |
Q945K2 | 2.0e-143 | 49.23 | (R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1 | [more] |
O24243 | 1.3e-139 | 48.75 | (R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0KCB1 | 2.9e-262 | 81.83 | GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447... | [more] |
A0A5D3C0K1 | 6.5e-262 | 81.29 | (R)-mandelonitrile lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... | [more] |
A0A1S3CHZ4 | 6.5e-262 | 81.29 | (R)-mandelonitrile lyase-like OS=Cucumis melo OX=3656 GN=LOC103500635 PE=3 SV=1 | [more] |
A0A6J1JYK6 | 1.3e-259 | 81.15 | (R)-mandelonitrile lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111488606 PE=3 S... | [more] |
A0A6J1GR64 | 8.7e-259 | 80.82 | (R)-mandelonitrile lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111456379 PE=3... | [more] |
Match Name | E-value | Identity | Description | |
AT1G73050.1 | 7.9e-220 | 69.38 | Glucose-methanol-choline (GMC) oxidoreductase family protein | [more] |
AT1G12570.1 | 1.1e-128 | 45.16 | Glucose-methanol-choline (GMC) oxidoreductase family protein | [more] |
AT1G72970.1 | 1.3e-126 | 45.71 | Glucose-methanol-choline (GMC) oxidoreductase family protein | [more] |
AT5G51950.1 | 3.1e-123 | 45.24 | Glucose-methanol-choline (GMC) oxidoreductase family protein | [more] |
AT5G51950.2 | 7.6e-122 | 45.21 | Glucose-methanol-choline (GMC) oxidoreductase family protein | [more] |