Sgr025058 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr025058 vs. NCBI nr
Match: XP_022136054.1 (ribonuclease 3-like protein 3 [Momordica charantia])

HSP 1 Score: 461.1 bits (1185), Expect = 7.0e-126
Identity = 235/273 (86.08%), Postives = 252/273 (92.31%), Query Frame = 0

Query: 1   MEEHAVDRFKQLCLQRRNAEVDDD-DRNPPESSESPPNLEEVEAILGYEFGDKSLLVEAF 60
           MEEH V+ F+QL LQ + A V+DD  RNPPESSESPP+LEEVEAILGYEF DK LL EAF
Sbjct: 1   MEEHGVELFEQLRLQAQGAVVNDDVRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAF 60

Query: 61  TDASYSPENCSSFERLEYVGDSVLNFLITKEQYFKYPSLSPGSLTRLRAANVDTEKLARV 120
           TD+SYSPENC SFERLEYVGDSVLNFLIT+EQYF  PSLSPGSLTRLRAANVDTEKLARV
Sbjct: 61  TDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARV 120

Query: 121 AITHGFHRYLRHNKPDLHKKIEEFAQGIEEYPLHSNGLIKAPKILADVVESTIGAVFTDS 180
           AITHGFHRYLRHNK DL KKIEEFA+G++EYPLHSNGLIKAPKILAD+VESTIGAVFTDS
Sbjct: 121 AITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDS 180

Query: 181 NSIDIVWTIFKGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDLWEQKKEVQVFIDK 240
           NS+D VWTIF+GLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVD WE+ KEVQVFIDK
Sbjct: 181 NSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDK 240

Query: 241 QLVGKGSYRKKLIAQNRAAKDALNNLDRVLAEL 273
           QLVGKGSYRKKLIAQNRAAKDALNNLD +L ++
Sbjct: 241 QLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQI 273

BLAST of Sgr025058 vs. NCBI nr
Match: XP_038888348.1 (ribonuclease 3-like protein 3 [Benincasa hispida])

HSP 1 Score: 412.1 bits (1058), Expect = 3.8e-111
Identity = 204/239 (85.36%), Postives = 220/239 (92.05%), Query Frame = 0

Query: 33  ESPPNLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFLITKEQY 92
           +S  +L+ +EAIL YEF DK LL EAFTDASYSPENCSSFERLEYVGDSVLNFLIT+EQY
Sbjct: 27  DSAASLDGIEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQY 86

Query: 93  FKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGIEEYPL 152
           F  P+LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLH KIEEF + IE+YPL
Sbjct: 87  FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHPKIEEFIKSIEDYPL 146

Query: 153 HSNGLIKAPKILADVVESTIGAVFTDSNSIDIVWTIFKGLMEPLITLKTIKRHPMTELTE 212
           HSNG+IKAPK+LAD+VESTIGAVF DSNSI+ VW IFK LMEPLITLKT+K+HPMTELTE
Sbjct: 147 HSNGMIKAPKVLADIVESTIGAVFADSNSIETVWKIFKDLMEPLITLKTMKKHPMTELTE 206

Query: 213 ICQKRNLKLEFVDLWEQKKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDRVLAE 272
           ICQKRNL LEFVD WE++KEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDRVL E
Sbjct: 207 ICQKRNLNLEFVDCWEERKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDRVLGE 265

BLAST of Sgr025058 vs. NCBI nr
Match: XP_023554575.1 (ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 398.3 bits (1022), Expect = 5.6e-107
Identity = 198/235 (84.26%), Postives = 215/235 (91.49%), Query Frame = 0

Query: 37  NLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFLITKEQYFKYP 96
           +L+ +EAIL YEF DK LL EAFTDASYSPENCSSFERLEYVGDSVLNFLIT+EQYF  P
Sbjct: 24  SLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83

Query: 97  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGIEEYPLHSNG 156
           +LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK DL +KIEEF++ IEEYPLHSNG
Sbjct: 84  NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143

Query: 157 LIKAPKILADVVESTIGAVFTDSNSIDIVWTIFKGLMEPLITLKTIKRHPMTELTEICQK 216
           LIKAPKILAD+VES IGAVFTDSNSID VWTIFKGLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 144 LIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203

Query: 217 RNLKLEFVDLWEQKKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDRVLAE 272
           RNLK EFVDLWE++KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N   +L +
Sbjct: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQ 258

BLAST of Sgr025058 vs. NCBI nr
Match: XP_022969213.1 (ribonuclease 3-like protein 3 [Cucurbita maxima])

HSP 1 Score: 397.5 bits (1020), Expect = 9.6e-107
Identity = 197/235 (83.83%), Postives = 215/235 (91.49%), Query Frame = 0

Query: 37  NLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFLITKEQYFKYP 96
           +L+ +EAIL YEF DK LL EAFTDASYSPENCSSFERLEYVGDSVLNFLIT+EQYF  P
Sbjct: 24  SLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83

Query: 97  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGIEEYPLHSNG 156
           +LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK DL +KIEEF++ IEEYPLHSNG
Sbjct: 84  NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143

Query: 157 LIKAPKILADVVESTIGAVFTDSNSIDIVWTIFKGLMEPLITLKTIKRHPMTELTEICQK 216
           +IKAPKILAD+VES IGAVFTDSNSID VWTIFKGLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 144 MIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203

Query: 217 RNLKLEFVDLWEQKKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDRVLAE 272
           RNLK EFVDLWE++KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N   +L +
Sbjct: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQ 258

BLAST of Sgr025058 vs. NCBI nr
Match: KAG6572281.1 (Endoribonuclease Dicer-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 397.1 bits (1019), Expect = 1.2e-106
Identity = 197/235 (83.83%), Postives = 215/235 (91.49%), Query Frame = 0

Query: 37  NLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFLITKEQYFKYP 96
           +L+ +EAIL YEF DK LL EAFTDASYSPENCSSFERLEYVGDSVLNFLIT+EQYF  P
Sbjct: 24  SLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83

Query: 97  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGIEEYPLHSNG 156
           +LSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK DL +KIEEF++ IEEYPLHSNG
Sbjct: 84  NLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143

Query: 157 LIKAPKILADVVESTIGAVFTDSNSIDIVWTIFKGLMEPLITLKTIKRHPMTELTEICQK 216
           LIKAPKILAD+VES IGAVFTDSNSID VWTIFKGLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 144 LIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203

Query: 217 RNLKLEFVDLWEQKKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDRVLAE 272
           RNLK EFVDLWE++KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N   +L +
Sbjct: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQ 258

BLAST of Sgr025058 vs. ExPASy Swiss-Prot
Match: Q69KJ0 (Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0358800 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.7e-59
Identity = 120/242 (49.59%), Postives = 169/242 (69.83%), Query Frame = 0

Query: 35  PPNLEEVEAILGYEFGDKSLLVEAFTDAS--YSPENCSSFERLEYVGDSVLNFLITKEQY 94
           P  +  VE++LGYEF DK+L+ EA T  S  Y      ++ERLEY+GD+VL  ++++E +
Sbjct: 36  PGEVAAVESLLGYEFRDKALVEEALTHGSFYYPYRPGVTYERLEYLGDAVLTCVVSREVF 95

Query: 95  FKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGIEEYPL 154
             Y  L PG LTRLRAANVD EKLARVA+ HG H +LRH  P+L  +I +F + +  YP+
Sbjct: 96  LTYGQLQPGPLTRLRAANVDKEKLARVAVVHGLHHFLRHKAPNLDGQITDFIEELSMYPI 155

Query: 155 HSNGLIKAPKILADVVESTIGAVFTDSN-SIDIVWTIFKGLMEPLITLKTIKRHPMTELT 214
           HSNGL+  PK+L DVVES IGA++ DSN + +IVW +F+ L +PLI+L+T+ +HP++EL 
Sbjct: 156 HSNGLLDPPKVLCDVVESLIGAIYCDSNFNQEIVWQVFQKLADPLISLETLGKHPVSELF 215

Query: 215 EICQKRNLKLEFV-DLWEQKKEVQVFIDKQLVGKGSY-RKKLIAQNRAAKDALNNLDRVL 272
           E CQK    ++ V D W++   V+V ID ++VG+ +Y +KK IAQNRAAK AL+ L   L
Sbjct: 216 EFCQKTRRGVKIVKDEWDKNLTVEVLIDGEMVGRATYAQKKEIAQNRAAKAALDKLKETL 275

BLAST of Sgr025058 vs. ExPASy Swiss-Prot
Match: Q9LTQ0 (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.6e-43
Identity = 101/247 (40.89%), Postives = 155/247 (62.75%), Query Frame = 0

Query: 29  PESSESPPNLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFLIT 88
           P SSE   ++E VE IL Y+F +KSLL EA T  S +  +  S+ERLE++GDS +   I+
Sbjct: 52  PVSSEM-ESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSAIGLAIS 111

Query: 89  KEQYFKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGI- 148
              Y  YPSL P  L+ LRAANV TEKLARV++ HG + +LR N P L +K++EF++ + 
Sbjct: 112 NYLYLTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVG 171

Query: 149 --EEYPLHSNGLIKAPKILADVVESTIGAVFTDSN-SIDIVWTIFKGLMEPLITLKTIKR 208
             ++  +   GL+KAPK+LAD+ ES  GAV+ D N  +  +W IF+GL+EP++TL  +++
Sbjct: 172 KEDDLSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQK 231

Query: 209 --HPMTELTEICQKRNLKLEFVDLWEQKKEVQV-FIDKQLVGKGSYRKKLIAQNRAAKDA 268
              P++ L ++C K   +++  +  +    + V ++D +L+  G    K IA+  AAK+A
Sbjct: 232 QPQPVSMLFKLCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEA 291

BLAST of Sgr025058 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.3e-42
Identity = 105/259 (40.54%), Postives = 153/259 (59.07%), Query Frame = 0

Query: 18   NAEVDDDDRNPPESSESPP------NLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSS 77
            +AE+D ++  PP+  + P       N + ++ +LG EF +K LLVEA T AS      S 
Sbjct: 1512 HAELDPEEIPPPKPYDIPESIMRSINFDTLKGVLGIEFQNKGLLVEAITHASRPSSGVSC 1571

Query: 78   FERLEYVGDSVLNFLITKEQYFKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRH 137
            ++RLE+VGD+VL+ LIT+  +F Y  L PG LT LRAA V+ E  ARVA+ H  H +LRH
Sbjct: 1572 YQRLEFVGDAVLDHLITRHLFFTYTDLPPGRLTDLRAAAVNNENFARVAVKHKLHVHLRH 1631

Query: 138  NKPDLHKKIEEFAQGIEEYPL----HSNGL--IKAPKILADVVESTIGAVFTDSN-SIDI 197
                L  +I EF + ++E  L    +S GL   KAPK+L D+VES  GA+F DS     +
Sbjct: 1632 GSSALETQIREFVKDVQEELLKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGYDTSV 1691

Query: 198  VWTIFKGLMEPLITLKTIKRHPMTELTEICQKRNLKLEF-VDLWEQKKEVQVFIDKQLVG 257
            VW +F+ L+ P++T +T+  HP+ EL E CQ++   LE+          V+VF+D   +G
Sbjct: 1692 VWKVFQPLLHPMVTPETLPMHPVRELQERCQQQAEGLEYKASRAGNIATVEVFVDGVQIG 1751

Query: 258  KGSYRKKLIAQNRAAKDAL 263
                 +K +AQ  AA++AL
Sbjct: 1752 VAQNPQKKMAQKLAARNAL 1770

BLAST of Sgr025058 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 1.5e-41
Identity = 108/254 (42.52%), Postives = 149/254 (58.66%), Query Frame = 0

Query: 27   NPPESSESPPNLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFL 86
            N PES     +   +E  L YEF +K LLVEA T AS      S ++RLE+VGD+VL+ L
Sbjct: 1548 NVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHL 1607

Query: 87   ITKEQYFKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQG 146
            IT+  +F Y SL PG LT LRAA V+ E  ARVA+ H  H YLRH    L K+I EF + 
Sbjct: 1608 ITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKE 1667

Query: 147  IE----EYPLHSNGL--IKAPKILADVVESTIGAVFTDS-NSIDIVWTIFKGLMEPLITL 206
            ++    +   +S GL   KAPK+L D+VES  GA+F DS       W +F+ L++P++T 
Sbjct: 1668 VQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTP 1727

Query: 207  KTIKRHPMTELTEICQKRNLKLEF-VDLWEQKKEVQVFIDKQLVGKGSYRKKLIAQNRAA 266
            +T+  HP+ EL E CQ++   LE+          V+VFID   VG     +K +AQ  AA
Sbjct: 1728 ETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAA 1787

Query: 267  KDALNNL-DRVLAE 272
            ++AL  L ++ +AE
Sbjct: 1788 RNALAALKEKEIAE 1801

BLAST of Sgr025058 vs. ExPASy Swiss-Prot
Match: Q6ATG6 (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 3.4e-38
Identity = 93/235 (39.57%), Postives = 139/235 (59.15%), Query Frame = 0

Query: 41  VEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFLITKEQYFKYPSLSP 100
           VE +L Y+F D  LL EA T  S++ ++  S++RLE+VGDS L    +   Y   P+L P
Sbjct: 40  VERLLRYQFRDGRLLEEALTHQSFA-DDAVSYQRLEFVGDSALGLAFSNFLYLTNPTLGP 99

Query: 101 GSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGIEEYP-------LH 160
           G L+ LRAAN+ TEKLARVA+ H  +  LR N P L   + +F + +++ P        +
Sbjct: 100 GPLSTLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPY 159

Query: 161 SNGLIKAPKILADVVESTIGAVFTDSN-SIDIVWTIFKGLMEPLITLKTIKRHPMTELTE 220
              ++KAPK+LAD+VE+   AV+ D    ++ +W + + L EP+IT +TI   P+T L E
Sbjct: 160 GGSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAETIDEQPVTMLHE 219

Query: 221 ICQKRNLKLEFVDLWEQ--KKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNL 266
           +CQK     +F   W++     V VF+  +LVG GS  +K+IA+  AA+DA   L
Sbjct: 220 LCQKHGKMAQF-KTWQKGGMTVVNVFVAGELVGIGSSEQKVIAKLNAARDATRKL 272

BLAST of Sgr025058 vs. ExPASy TrEMBL
Match: A0A6J1C375 (ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 3.4e-126
Identity = 235/273 (86.08%), Postives = 252/273 (92.31%), Query Frame = 0

Query: 1   MEEHAVDRFKQLCLQRRNAEVDDD-DRNPPESSESPPNLEEVEAILGYEFGDKSLLVEAF 60
           MEEH V+ F+QL LQ + A V+DD  RNPPESSESPP+LEEVEAILGYEF DK LL EAF
Sbjct: 1   MEEHGVELFEQLRLQAQGAVVNDDVRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAF 60

Query: 61  TDASYSPENCSSFERLEYVGDSVLNFLITKEQYFKYPSLSPGSLTRLRAANVDTEKLARV 120
           TD+SYSPENC SFERLEYVGDSVLNFLIT+EQYF  PSLSPGSLTRLRAANVDTEKLARV
Sbjct: 61  TDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARV 120

Query: 121 AITHGFHRYLRHNKPDLHKKIEEFAQGIEEYPLHSNGLIKAPKILADVVESTIGAVFTDS 180
           AITHGFHRYLRHNK DL KKIEEFA+G++EYPLHSNGLIKAPKILAD+VESTIGAVFTDS
Sbjct: 121 AITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDS 180

Query: 181 NSIDIVWTIFKGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDLWEQKKEVQVFIDK 240
           NS+D VWTIF+GLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVD WE+ KEVQVFIDK
Sbjct: 181 NSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDK 240

Query: 241 QLVGKGSYRKKLIAQNRAAKDALNNLDRVLAEL 273
           QLVGKGSYRKKLIAQNRAAKDALNNLD +L ++
Sbjct: 241 QLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQI 273

BLAST of Sgr025058 vs. ExPASy TrEMBL
Match: A0A6J1I0C1 (ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 4.6e-107
Identity = 197/235 (83.83%), Postives = 215/235 (91.49%), Query Frame = 0

Query: 37  NLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFLITKEQYFKYP 96
           +L+ +EAIL YEF DK LL EAFTDASYSPENCSSFERLEYVGDSVLNFLIT+EQYF  P
Sbjct: 24  SLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83

Query: 97  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGIEEYPLHSNG 156
           +LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK DL +KIEEF++ IEEYPLHSNG
Sbjct: 84  NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143

Query: 157 LIKAPKILADVVESTIGAVFTDSNSIDIVWTIFKGLMEPLITLKTIKRHPMTELTEICQK 216
           +IKAPKILAD+VES IGAVFTDSNSID VWTIFKGLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 144 MIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203

Query: 217 RNLKLEFVDLWEQKKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDRVLAE 272
           RNLK EFVDLWE++KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N   +L +
Sbjct: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQ 258

BLAST of Sgr025058 vs. ExPASy TrEMBL
Match: A0A6J1GKQ4 (ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 1.0e-106
Identity = 197/235 (83.83%), Postives = 215/235 (91.49%), Query Frame = 0

Query: 37  NLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFLITKEQYFKYP 96
           +L+ +EAIL YEF DK LL EAFTDASYSPENCSSFERLEYVGDSVLNFLIT+EQYF  P
Sbjct: 24  SLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83

Query: 97  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGIEEYPLHSNG 156
           +LSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK DL +KIEEF++ IEEYPLHSNG
Sbjct: 84  NLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143

Query: 157 LIKAPKILADVVESTIGAVFTDSNSIDIVWTIFKGLMEPLITLKTIKRHPMTELTEICQK 216
           LIKAPKILAD+VES IGAVFTDSNSID VWTIFKGLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 144 LIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203

Query: 217 RNLKLEFVDLWEQKKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDRVLAE 272
           RNLK EFVDLWE++KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N   +L +
Sbjct: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQ 258

BLAST of Sgr025058 vs. ExPASy TrEMBL
Match: A0A0A0K1J8 (RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 PE=4 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 2.5e-105
Identity = 193/235 (82.13%), Postives = 214/235 (91.06%), Query Frame = 0

Query: 32  SESPPNLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFLITKEQ 91
           S+SP +L+ +EAIL YEF DK LL EAFTDASYSPENCSSFERLEYVGDSVLNFLIT+EQ
Sbjct: 28  SDSPASLDGIEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQ 87

Query: 92  YFKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGIEEYP 151
           YF  P+LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK DLH+KIEEF + IEEYP
Sbjct: 88  YFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHQKIEEFGRSIEEYP 147

Query: 152 LHSNGLIKAPKILADVVESTIGAVFTDSNSIDIVWTIFKGLMEPLITLKTIKRHPMTELT 211
           LHSNG+IKAPK+LAD+VESTIGAVF DSN ID VW IFK LMEPLITLKT+K+HPMTELT
Sbjct: 148 LHSNGMIKAPKVLADIVESTIGAVFADSNFIDTVWKIFKELMEPLITLKTMKKHPMTELT 207

Query: 212 EICQKRNLKLEFVDLWEQKKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLD 267
           E+CQKR+LKLEF D WE+ KE++V I+KQ+VGKGSYRKKLIAQNRAAK+AL NLD
Sbjct: 208 EMCQKRSLKLEFRDRWEETKEIEVLIEKQVVGKGSYRKKLIAQNRAAKNALENLD 262

BLAST of Sgr025058 vs. ExPASy TrEMBL
Match: A0A1Q3BNT8 (Ribonuclease_3 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_13208 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 5.9e-86
Identity = 164/237 (69.20%), Postives = 199/237 (83.97%), Query Frame = 0

Query: 38  LEEVEAILGYEFGDKSLLVEAFTDASY-SPENCSSFERLEYVGDSVLNFLITKEQYFKYP 97
           LE VE ILGY+FG+KSLL EAFT ASY SP+ C+S+ERLE+VGDSVLN L TKEQYF YP
Sbjct: 1   LEGVEEILGYKFGNKSLLEEAFTHASYTSPDQCASYERLEFVGDSVLNLLFTKEQYFLYP 60

Query: 98  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGIEEYPLHSNG 157
           +L PG LTRLRAANVDTEKLARV+I HG HRYL H KP L ++I+EF Q I +YPLHSNG
Sbjct: 61  NLQPGDLTRLRAANVDTEKLARVSIRHGLHRYLCHKKPLLEQQIQEFTQAILDYPLHSNG 120

Query: 158 LIKAPKILADVVESTIGAVFTDSN-SIDIVWTIFKGLMEPLITLKTIKRHPMTELTEICQ 217
           L+  PK+LAD+VESTIGAVF DSN SIDIVW +FKGL+EP+I+++T+KRHP+TEL E+CQ
Sbjct: 121 LVDPPKVLADIVESTIGAVFIDSNLSIDIVWKVFKGLLEPIISVETLKRHPVTELYEVCQ 180

Query: 218 KRNLKLEFVDLWEQKKEVQVFIDKQLVGKGSY-RKKLIAQNRAAKDALNNLDRVLAE 272
           K+NLK++FVDLW++    +VFID QLVG+ +Y  KK IA NRAAKDALNN+ ++L E
Sbjct: 181 KKNLKVKFVDLWKESTAFEVFIDDQLVGRDTYGTKKEIAHNRAAKDALNNMWKILNE 237

BLAST of Sgr025058 vs. TAIR 10
Match: AT4G15417.1 (RNAse II-like 1 )

HSP 1 Score: 247.7 bits (631), Expect = 1.1e-65
Identity = 131/256 (51.17%), Postives = 172/256 (67.19%), Query Frame = 0

Query: 21  VDDDDRNPPESSESPPNLEEVEA---ILGYEFGDKSLLVEAFTDASYSPENCSSFERLEY 80
           +D  D      S  P  L E+E+   IL Y+F DKSLL++AFTDASY  +   S+E LE 
Sbjct: 9   IDGKDNTKTIGSADPDQLMEIESLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLEL 68

Query: 81  VGDSVLNFLITKEQYFKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLH 140
           +GDS+LN  I  +    YP  +PG LT+LRA NVDTEKLARVA+ H  + YLRH KP L 
Sbjct: 69  LGDSILNMGIIYDFIKLYPKEAPGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLE 128

Query: 141 KKIEEFAQGIEEYPLHSNGLIKAPKILADVVESTIGAVFTDSNSIDIVWTIFKGLMEPLI 200
           ++I EF + +E+YPLHSNGL+K PK+LAD+VESTIGA+F D NS + VW + K L+EP+I
Sbjct: 129 EQILEFVEAMEKYPLHSNGLLKVPKVLADIVESTIGAIFMDCNSTETVWKVIKPLLEPII 188

Query: 201 TLKTIKRHPMTELTEICQKRNLKLEFVDLWEQKKEVQVFIDKQLVGKGSYR-KKLIAQNR 260
            L  +K HPMTEL E+CQKRNLKL   D WE+ +     I+ + VG G +  KK  A+N 
Sbjct: 189 HLDKMKSHPMTELNEMCQKRNLKLTSKDTWEENQTYCFHIEDKFVGCGHHPVKKETARNF 248

Query: 261 AAKDALNNLDRVLAEL 273
           AAK+A++N  +   +L
Sbjct: 249 AAKNAIDNFAKFFGDL 264

BLAST of Sgr025058 vs. TAIR 10
Match: AT3G20420.1 (RNAse THREE-like protein 2 )

HSP 1 Score: 177.9 bits (450), Expect = 1.1e-44
Identity = 101/247 (40.89%), Postives = 155/247 (62.75%), Query Frame = 0

Query: 29  PESSESPPNLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFLIT 88
           P SSE   ++E VE IL Y+F +KSLL EA T  S +  +  S+ERLE++GDS +   I+
Sbjct: 52  PVSSEM-ESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSAIGLAIS 111

Query: 89  KEQYFKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQGI- 148
              Y  YPSL P  L+ LRAANV TEKLARV++ HG + +LR N P L +K++EF++ + 
Sbjct: 112 NYLYLTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVG 171

Query: 149 --EEYPLHSNGLIKAPKILADVVESTIGAVFTDSN-SIDIVWTIFKGLMEPLITLKTIKR 208
             ++  +   GL+KAPK+LAD+ ES  GAV+ D N  +  +W IF+GL+EP++TL  +++
Sbjct: 172 KEDDLSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQK 231

Query: 209 --HPMTELTEICQKRNLKLEFVDLWEQKKEVQV-FIDKQLVGKGSYRKKLIAQNRAAKDA 268
              P++ L ++C K   +++  +  +    + V ++D +L+  G    K IA+  AAK+A
Sbjct: 232 QPQPVSMLFKLCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEA 291

BLAST of Sgr025058 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 171.4 bits (433), Expect = 1.0e-42
Identity = 108/254 (42.52%), Postives = 149/254 (58.66%), Query Frame = 0

Query: 27   NPPESSESPPNLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFL 86
            N PES     +   +E  L YEF +K LLVEA T AS      S ++RLE+VGD+VL+ L
Sbjct: 1548 NVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHL 1607

Query: 87   ITKEQYFKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQG 146
            IT+  +F Y SL PG LT LRAA V+ E  ARVA+ H  H YLRH    L K+I EF + 
Sbjct: 1608 ITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKE 1667

Query: 147  IE----EYPLHSNGL--IKAPKILADVVESTIGAVFTDS-NSIDIVWTIFKGLMEPLITL 206
            ++    +   +S GL   KAPK+L D+VES  GA+F DS       W +F+ L++P++T 
Sbjct: 1668 VQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTP 1727

Query: 207  KTIKRHPMTELTEICQKRNLKLEF-VDLWEQKKEVQVFIDKQLVGKGSYRKKLIAQNRAA 266
            +T+  HP+ EL E CQ++   LE+          V+VFID   VG     +K +AQ  AA
Sbjct: 1728 ETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAA 1787

Query: 267  KDALNNL-DRVLAE 272
            ++AL  L ++ +AE
Sbjct: 1788 RNALAALKEKEIAE 1801

BLAST of Sgr025058 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 171.4 bits (433), Expect = 1.0e-42
Identity = 108/254 (42.52%), Postives = 149/254 (58.66%), Query Frame = 0

Query: 27   NPPESSESPPNLEEVEAILGYEFGDKSLLVEAFTDASYSPENCSSFERLEYVGDSVLNFL 86
            N PES     +   +E  L YEF +K LLVEA T AS      S ++RLE+VGD+VL+ L
Sbjct: 1549 NVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHL 1608

Query: 87   ITKEQYFKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFAQG 146
            IT+  +F Y SL PG LT LRAA V+ E  ARVA+ H  H YLRH    L K+I EF + 
Sbjct: 1609 ITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKE 1668

Query: 147  IE----EYPLHSNGL--IKAPKILADVVESTIGAVFTDS-NSIDIVWTIFKGLMEPLITL 206
            ++    +   +S GL   KAPK+L D+VES  GA+F DS       W +F+ L++P++T 
Sbjct: 1669 VQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTP 1728

Query: 207  KTIKRHPMTELTEICQKRNLKLEF-VDLWEQKKEVQVFIDKQLVGKGSYRKKLIAQNRAA 266
            +T+  HP+ EL E CQ++   LE+          V+VFID   VG     +K +AQ  AA
Sbjct: 1729 ETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAA 1788

Query: 267  KDALNNL-DRVLAE 272
            ++AL  L ++ +AE
Sbjct: 1789 RNALAALKEKEIAE 1802

BLAST of Sgr025058 vs. TAIR 10
Match: AT3G03300.1 (dicer-like 2 )

HSP 1 Score: 152.9 bits (385), Expect = 3.8e-37
Identity = 88/202 (43.56%), Postives = 124/202 (61.39%), Query Frame = 0

Query: 26   RNPPESSESPPNLEEVEAILGYEFGDKSLLVEAFTDASY-SPENCSSFERLEYVGDSVLN 85
            R+ P  +E   N+  +E++L Y F DKSLLVEA T  SY  PE    ++RLE++GDSVL+
Sbjct: 1137 RDSPIQAEKLVNVGYMESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLD 1196

Query: 86   FLITKEQYFKYPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKPDLHKKIEEFA 145
            +LITK  Y KYP LSPG LT +R+A+V+ E  A VA+    H+++ +    LHK I    
Sbjct: 1197 YLITKHLYDKYPCLSPGLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTV 1256

Query: 146  QGIEEYPLHS----NGLIKAPKILADVVESTIGAVFTDSN-SIDIVWTIFKGLMEPLITL 205
               E+  L S       I  PK+L DV+ES  GA+F DS  + ++V+   K L+  +IT 
Sbjct: 1257 SEFEQSSLQSTFGWESDISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITP 1316

Query: 206  KTIKRHPMTELTEICQKRNLKL 222
            +T+K HP+ ELTE+CQK   +L
Sbjct: 1317 ETVKLHPVRELTELCQKWQFEL 1338