Sgr023622 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr023622
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	indole-3-acetic acid-amido synthetase GH3.10
Location	tig00000892: 5078672 .. 5082387 (+)
RNA-Seq Expression	Sgr023622
Synteny	Sgr023622

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCTCCTCAAGGACTAGGAGTGACGAATGATCACTTGACTGAACACGACATCATTGATTGGTTCGAGCAAGTCTCCAAGCACGCAAGCCTTGTTCAATCTCGCACTCTCCGTCAGATTCTGGATCAGAATTCTGGTGTGGAGTACCTGAAGAAATGGCTTGGAGATGTCAATATCCATGAAATCCCAGATGACGACTTTGCATTGGAATCACTCTTCACTTCCTTGGTACCCCTTTCTTCTCATGCCGATTTGGAGCCTTATCTTCAACGAATCGCAGATGGGGACTCCGCTCCATTATTAACTCAACAACCCATCAAAACTCTCTCTTTAAGGTACCCCCATTAAACTACTCTCTTCAATCTTTCTTCTTCTTCCTGCTTTGCTGAGTTTCTTTTGTTGGTCCGAGGACAAGGGTGGGGAGGGGGCCTCATTTCATTATAGCTGCTAGAAATGTATTTCGGAGCATAGAATTTGAGATACCTTTGGAGCCAAGTGGATAGCTTTTCTTCTTCTTCTTCCTTCTCTTTTTTCCCCTTTCATTTTCTTCCTTATCAATCTCTGAAGGGTATCTAATCACAAGTTGTTCAAGCACAAGGTCTTGTTCTCATGCACTTTAGAGAGAGAGAGAGAGAGAGAGAGAGGTTTCCCGCCCTTTTGCTAGTGAAGTGTGTCTTACTTACTGAGTGACCATGGTCTCTAATTTATCCCGCTGCAGTTCTGGCACCACGGAAGGCAGACAGAAGTACGTACCTTTTACTCGCCATAGTGCGCAGACTACTCTTCTAATCTTTCGCTTAGCAGCTGCTTACAGATCCAGGTACCAACCCCTCTCCATCATCTCATATAACATTACATTAATGTTTCTCTCTCAAACTAAAACCAACACATGGCCTTCTTTTTTTAAATCATCTACTACGGTCCCTTTTTATTACTTCTACAAGGGACCATCTCACCTGTGGTTGGTTGATTGGTCAATAATGCATAAATTAGGGTTTATCCCATAAGGGAAGGAGGGAAGATTCTGGAGTTCATTTATAGCAGCAAACAGACCAGAACCAAGGGCGGCTAATAACTGGAACCGCCACCACCCACTACTATGCGAGTGAAGAATTTAAGATCAAGCAGCAGAAAACCAAGTCCTTCACTTGCAGCCCCGAAGAAGTCATTTCCGGAGGAGACTACAAGGAATCCACATACTGCCATCTCCTCCTGGGCCTCTTCTTCTGTGATGAAGTGGAGTTTGTTACCTCTACTTTTGCTTACAGCATAGTGCAGGCCTTCGATGAATTCGAAGAGGTTTGGGTTGAGATTTGCCGAGACATAAGGGAAGGCACACTCTGCTCAAGAATCACTCTTCCCAAAATGCGAAAGGCTGTTTTGAACGTCCTCTCTCCGAACCCATGTCTGGCTTCCACAATACAAGCCTGCTGCCAACAACTCCAAAACCAGGACTGGTTTGGTTTGATTCCGAAGCTGTGGCCCAATTCCAAGTATGTGTATTCTATAATGACGGGATCCATGCAGCCATATTTGAAAAAACTAAGGCACTATGCTGGGGGGCTGCCATTAGTGAGTGCTGATTATGGATCAACTGAGAGTTGGATTGGGGTGAATGTGGACCCTTCTCTACCACCAGAAAACGTTACTTTTGCCGTAATTCCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACAGGAAGATTGCAGTTCCTACGATGCCATTGATGACTTCTTGGAAGGTGAACCAGTACCGCTGTCTCAAGTCAAGATTGGACAAGAGTATGAGGTTGTTCTTACTACTTTCACAGGTCAGTTGTCTTTTCAGTTCAACCCGCAAAAAATGTGTAACATAAAATAATATCTACATATTTCCGTTTGATAGGCCTTTCCTTCTTTCGTATGATCTGGATTTGTCTCCTTATTAATTATTTCATTTGACACCTAATTAATCCCTTTCCAGTACTCATTTAATCGCTTTTATAAATGAGGATGGGTTTCTTTATGGGTCACTTTCTGGAGCTGGTTGGCTCTTTGTTTTTTTTTTTTTTTTTGTCTACAACAATCTCATTACGGCTTGATTTTTCTAGTACGTTGATTGTCCATGATTTCCATGACAAAATATCTCTGAGAAGATTATTATTATTATATGGTAATCATAAATCATTTGATAAGTAAATTGTCAGTATCCTCAATTTGACGGACAAGGGATAGATTTCTTCTGTGGCCCCCGGAACCTACCTTATTTTGGGTCTATGTAAAATGAATTTTAATTTGAAAATGACCACACTAACTTCCTCCTCAATTAAATTTATTACCATTAAATCACTACTAAATTTTAAAAATAATCAAATAATCAGATCATGATGAGTTTAGTTATTCAATACATTTTGCATATTAACAGTAACATATTCCTCTTCTGATTTTTATATGCATCAACCCCGCCAGGAGTTCCACATGTAGATTGATTCTCATAACATTTTATATGAGAAAAAAAAATTCAATTTTTAAAGTGAGTCTGCCAATTCCATCAAATGACTTTAGATTTGGACTTGGATGACTGTTGTAACTTTTACCTTTATTAAGCTTTTGTTTAAATAATCATTAAAAAAATGTACTCATGTAAGTTCTATGGATTACAAACATAAACCTAGTACGAAATTGATGAGATTTGAGAGAGAGATGATTTTAGGGCTAAATTTTGTTGAAATTGATGAATTTTTTTAGAAATTTATTCAATGGTAGATTTTGTGCATTAATTTTATAGAGCTTGGTTTTGTGCATTAATTTGTTAGGCCTTACTTTTTGTTGAAAATAGTGAGTTAGATGATTGTTTTTTGTTGAAATTAGTAGGCAAATGTAATAGAAGCCTAAAAAAAAATGAAAAAATTTTAATTGGATGAAATTGAAAATTTAGAATAAAAATTGATTGGGGGGGGGGGGGTGAAAATTGAATTTTATACCCATTTATGTGTTACATTACATCAAACGTAACACAGCAGCCGACAAGGATAGTTTCTTAAACATTAAAGGGATTGTAACATCTTGAGAAAGAGAAAAAAGAAAACTACATGTTCTGGTGTTAATGTTTTTTGATGTTAGGTTGTGGTAAATAGTTCTGTTCCTGAATTAAGCGCTTACTAGACATTATTGGATCCAGGGCTTTACAGATGCAGGTTAGGCGACGTCGTAGAAGTGGCTGACTTCCACAACAAGACGCCCAAACTCAACTTTATATGCAGAAGAAAGCTTGTGTTGACCGTAAATATAGACAAAAACACCGAGAAAGATCTGCAATTGGTGGTTGAAAGAGGGTCCCAGCTGCTGGCCGACCAAAGTGGAGCCGAACTAGTTGACTTCACGAGCCATGCTAACTTGGCCGACCGTGGCCAACCAGGTCACTACGTCATCTTCTGGGAAGTCAAAGGAGATGTGGAGGATAGAGTTCTGGGAGAGTGCTGCAGAGAGATGGACGCTGCATTCGTCGACTATGGCTACGTTGTGTCCAGGAGATCCAATTCCATTGGACCTCTTGAGCTTCGCATTGTGGAGAGAGGGACTTTCAAGAAGATTTTGGAACATTATATTGGAAATGGAGCCGCACTTAGCCAATTTAAGACCCCCAGATGCACCAACAACCAGGTTCTTCTCACAATTCTCAATCTCTCCACCGTTAAACGCTTTCGCAGCACCGCATATGCCTGA

mRNA sequence

ATGGAGGCTCCTCAAGGACTAGGAGTGACGAATGATCACTTGACTGAACACGACATCATTGATTGGTTCGAGCAAGTCTCCAAGCACGCAAGCCTTGTTCAATCTCGCACTCTCCGTCAGATTCTGGATCAGAATTCTGGTGTGGAGTACCTGAAGAAATGGCTTGGAGATGTCAATATCCATGAAATCCCAGATGACGACTTTGCATTGGAATCACTCTTCACTTCCTTGGTACCCCTTTCTTCTCATGCCGATTTGGAGCCTTATCTTCAACGAATCGCAGATGGGGACTCCGCTCCATTATTAACTCAACAACCCATCAAAACTCTCTCTTTAAGTTCTGGCACCACGGAAGGCAGACAGAAGTACGTACCTTTTACTCGCCATAGTGCGCAGACTACTCTTCTAATCTTTCGCTTAGCAGCTGCTTACAGATCCAGACAACAGGAAGATTGCAGTTCCTACGATGCCATTGATGACTTCTTGGAAGGTGAACCAGTACCGCTGTCTCAAGTCAAGATTGGACAAGAGTATGAGGTTGTTCTTACTACTTTCACAGGGCTTTACAGATGCAGGTTAGGCGACGTCGTAGAAGTGGCTGACTTCCACAACAAGACGCCCAAACTCAACTTTATATGCAGAAGAAAGCTTGTGTTGACCGTAAATATAGACAAAAACACCGAGAAAGATCTGCAATTGGTGGTTGAAAGAGGGTCCCAGCTGCTGGCCGACCAAAGTGGAGCCGAACTAGTTGACTTCACGAGCCATGCTAACTTGGCCGACCGTGGCCAACCAGGTCACTACGTCATCTTCTGGGAAGTCAAAGGAGATGTGGAGGATAGAGTTCTGGGAGAGTGCTGCAGAGAGATGGACGCTGCATTCGTCGACTATGGCTACGTTGTGTCCAGGAGATCCAATTCCATTGGACCTCTTGAGCTTCGCATTGTGGAGAGAGGGACTTTCAAGAAGATTTTGGAACATTATATTGGAAATGGAGCCGCACTTAGCCAATTTAAGACCCCCAGATGCACCAACAACCAGGTTCTTCTCACAATTCTCAATCTCTCCACCGTTAAACGCTTTCGCAGCACCGCATATGCCTGA

Coding sequence (CDS)

ATGGAGGCTCCTCAAGGACTAGGAGTGACGAATGATCACTTGACTGAACACGACATCATTGATTGGTTCGAGCAAGTCTCCAAGCACGCAAGCCTTGTTCAATCTCGCACTCTCCGTCAGATTCTGGATCAGAATTCTGGTGTGGAGTACCTGAAGAAATGGCTTGGAGATGTCAATATCCATGAAATCCCAGATGACGACTTTGCATTGGAATCACTCTTCACTTCCTTGGTACCCCTTTCTTCTCATGCCGATTTGGAGCCTTATCTTCAACGAATCGCAGATGGGGACTCCGCTCCATTATTAACTCAACAACCCATCAAAACTCTCTCTTTAAGTTCTGGCACCACGGAAGGCAGACAGAAGTACGTACCTTTTACTCGCCATAGTGCGCAGACTACTCTTCTAATCTTTCGCTTAGCAGCTGCTTACAGATCCAGACAACAGGAAGATTGCAGTTCCTACGATGCCATTGATGACTTCTTGGAAGGTGAACCAGTACCGCTGTCTCAAGTCAAGATTGGACAAGAGTATGAGGTTGTTCTTACTACTTTCACAGGGCTTTACAGATGCAGGTTAGGCGACGTCGTAGAAGTGGCTGACTTCCACAACAAGACGCCCAAACTCAACTTTATATGCAGAAGAAAGCTTGTGTTGACCGTAAATATAGACAAAAACACCGAGAAAGATCTGCAATTGGTGGTTGAAAGAGGGTCCCAGCTGCTGGCCGACCAAAGTGGAGCCGAACTAGTTGACTTCACGAGCCATGCTAACTTGGCCGACCGTGGCCAACCAGGTCACTACGTCATCTTCTGGGAAGTCAAAGGAGATGTGGAGGATAGAGTTCTGGGAGAGTGCTGCAGAGAGATGGACGCTGCATTCGTCGACTATGGCTACGTTGTGTCCAGGAGATCCAATTCCATTGGACCTCTTGAGCTTCGCATTGTGGAGAGAGGGACTTTCAAGAAGATTTTGGAACATTATATTGGAAATGGAGCCGCACTTAGCCAATTTAAGACCCCCAGATGCACCAACAACCAGGTTCTTCTCACAATTCTCAATCTCTCCACCGTTAAACGCTTTCGCAGCACCGCATATGCCTGA

Protein sequence

MEAPQGLGVTNDHLTEHDIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSLVPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLIFRLAAAYRSRQQEDCSSYDAIDDFLEGEPVPLSQVKIGQEYEVVLTTFTGLYRCRLGDVVEVADFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHANLADRGQPGHYVIFWEVKGDVEDRVLGECCREMDAAFVDYGYVVSRRSNSIGPLELRIVERGTFKKILEHYIGNGAALSQFKTPRCTNNQVLLTILNLSTVKRFRSTAYA

Homology

[Close]

BLAST of Sgr023622 vs. NCBI nr
Match: XP_022986272.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima])

HSP 1 Score: 510.8 bits (1314), Expect = 1.0e-140
Identity = 302/589 (51.27%), Postives = 324/589 (55.01%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSL 77
           D++DWFE+VS  A + QSRTLR+IL QN GVEYL +WLGDVNIH+IP DDFALESLFTSL
Sbjct: 7   DMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIP-DDFALESLFTSL 66

Query: 78  VPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 137
           VPLSSHAD EP+LQRIADGDS+PLLTQQPI+TLSLSSGTTEGRQKYVPFT HSAQTTLLI
Sbjct: 67  VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 126

Query: 138 FRLAAAYRSR-------------------------------------------------- 197
           FR+AAAYRSR                                                  
Sbjct: 127 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 186

Query: 198 ------------------------------------------------------------ 257
                                                                       
Sbjct: 187 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 246

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 247 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCKYVY 306

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 307 SIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYFE 366

[Close]

BLAST of Sgr023622 vs. NCBI nr
Match: XP_022943441.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata])

HSP 1 Score: 508.8 bits (1309), Expect = 3.8e-140
Identity = 301/589 (51.10%), Postives = 322/589 (54.67%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSL 77
           D++DWFE+VS  A + Q RTLR+IL QN GVEYL +WLGDVNIH+IP DDFALESLFTSL
Sbjct: 7   DMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIP-DDFALESLFTSL 66

Query: 78  VPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 137
           +PLSSHAD EP+LQRIADGDS+PLLTQQPI+TLSLSSGTTEGRQKYVPFT HSAQTTLLI
Sbjct: 67  IPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 126

Query: 138 FRLAAAYRSR-------------------------------------------------- 197
           FR+AAAYRSR                                                  
Sbjct: 127 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 186

Query: 198 ------------------------------------------------------------ 257
                                                                       
Sbjct: 187 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 246

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 247 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 306

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 307 SIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 366

[Close]

BLAST of Sgr023622 vs. NCBI nr
Match: XP_023512179.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508.8 bits (1309), Expect = 3.8e-140
Identity = 302/589 (51.27%), Postives = 322/589 (54.67%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSL 77
           D++DWFE+VS  A + Q RTLR+IL QN GVEYL +WLGDVNIH+IP DDFALESLFTSL
Sbjct: 7   DMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIP-DDFALESLFTSL 66

Query: 78  VPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 137
           VPLSSHAD EP+LQRIADGDS+PLLTQQPI+TLSLSSGTTEGRQKYVPFT HSAQTTLLI
Sbjct: 67  VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 126

Query: 138 FRLAAAYRSR-------------------------------------------------- 197
           FR+AAAYRSR                                                  
Sbjct: 127 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 186

Query: 198 ------------------------------------------------------------ 257
                                                                       
Sbjct: 187 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 246

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 247 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 306

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 307 SIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 366

[Close]

BLAST of Sgr023622 vs. NCBI nr
Match: KAG6570848.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 508.4 bits (1308), Expect = 5.0e-140
Identity = 301/589 (51.10%), Postives = 322/589 (54.67%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSL 77
           D++DWFE+VS  A + Q RTLR+IL QN GVEYL +WLGDVNIH+IP DDFALESLFTSL
Sbjct: 7   DMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIP-DDFALESLFTSL 66

Query: 78  VPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 137
           +PLSSHAD EP+LQRIADGDS+PLLTQQPI+TLSLSSGTTEGRQKYVPFT HSAQTTLLI
Sbjct: 67  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 126

Query: 138 FRLAAAYRSR-------------------------------------------------- 197
           FR+AAAYRSR                                                  
Sbjct: 127 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 186

Query: 198 ------------------------------------------------------------ 257
                                                                       
Sbjct: 187 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 246

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 247 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 306

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 307 SIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 366

[Close]

BLAST of Sgr023622 vs. NCBI nr
Match: KAG7010696.1 (Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 501.1 bits (1289), Expect = 8.0e-138
Identity = 300/604 (49.67%), Postives = 321/604 (53.15%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSL 77
           D++DWFE+VS  A + Q RTLR+IL QN GVEYL +WLGDVNIH+IP DDFALESLFTSL
Sbjct: 7   DMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIP-DDFALESLFTSL 66

Query: 78  VPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 137
           +PLSSHAD EP+LQRIADGDS+PLLTQQPI+TLSLSSGTTEGRQKYVPFT HSAQTTLLI
Sbjct: 67  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 126

Query: 138 FRLAAAYRSR-------------------------------------------------- 197
           FR+AAAYRSR                                                  
Sbjct: 127 FRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHY 186

Query: 198 ------------------------------------------------------------ 257
                                                                       
Sbjct: 187 YASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQ 246

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 247 AFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDW 306

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 307 LDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPP 366

[Close]

BLAST of Sgr023622 vs. ExPASy Swiss-Prot
Match: Q9ZNS2 (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.1e-112
Identity = 247/585 (42.22%), Postives = 296/585 (50.60%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSL 77
           D+I WFE VS++A  VQS TLR+IL+ NSGVEYL+KWLG V++ ++  DD+ LE+LFTSL
Sbjct: 10  DVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM--DDYTLETLFTSL 69

Query: 78  VPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 137
           VP+ SHADL+PY+QRIADG+++PLLTQ+PI  LSLSSGTTEGRQKYVPFTRHSAQTTL I
Sbjct: 70  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 129

Query: 138 FRLAAAYRSR-------------------------------------------QQE---- 197
           FRL+AAYRSR                                           +QE    
Sbjct: 130 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 189

Query: 198 -DCSSYDAID-------------------------------------------------- 257
             CS  + I                                                   
Sbjct: 190 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 249

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 250 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 309

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 310 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 369

[Close]

BLAST of Sgr023622 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 6.0e-72
Identity = 178/574 (31.01%), Postives = 253/574 (44.08%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSL 77
           ++I+ FE ++K A  +Q  TL++IL++N G EYL++W  +     +          F + 
Sbjct: 15  EVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQWGLNGKTDSLS---------FKNC 74

Query: 78  VPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 137
           +P+ +H DLEPY+ RIADGD +P+LT +PI T+SLSSGTT+G+ K+VPF     ++T+ I
Sbjct: 75  IPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTMQI 134

Query: 138 FRLAAAYRSRQ----------------------------------------------QED 197
           F+ +  +R+R+                                              Q  
Sbjct: 135 FKTSFVFRNREFPVVNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMQTP 194

Query: 198 CSSYDAID---------------------------------------------------- 257
           C S D +                                                     
Sbjct: 195 CCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQELVTNI 254

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 255 REGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRLSNWYGLIPELFPNTRYIYGIM 314

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 315 TGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGYFEFIP 374

[Close]

BLAST of Sgr023622 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 270.4 bits (690), Expect = 3.0e-71
Identity = 184/578 (31.83%), Postives = 250/578 (43.25%), Query Frame = 0


Query: 16  EHDIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFT 75
           +  +I+ FE ++K A  +Q  TL++IL+QN G EYL+ W   +N    P         F 
Sbjct: 14  QEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLW--GLNGRTDP-------QTFK 73

Query: 76  SLVPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTL 135
           + VP+ +H DLEPY+QRIADGD +P+LT +PI+T+SLSSGTT+G+ K+VPF     ++T+
Sbjct: 74  NCVPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMESTM 133

Query: 136 LIFRLAAAYRSRQ----------------------------------------------- 195
            IF+ + A+R+R+                                               
Sbjct: 134 QIFKTSFAFRNREFPIGNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMC 193

Query: 196 ------------------------------------------------------------ 255
                                                                       
Sbjct: 194 TPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVWEALVV 253

Query: 256 ----------------------------------QEDCSS----YDAIDDFL-------- 315
                                                CS     Y  I D          
Sbjct: 254 DIREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRLSNWYGLIPDLFPNTRYIYG 313

Query: 316 ------------------------------EG---------------------------- 367
                                         EG                            
Sbjct: 314 IMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNIGYFEF 373

[Close]

BLAST of Sgr023622 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 8.7e-63
Identity = 173/585 (29.57%), Postives = 241/585 (41.20%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKW-LGDVNIHEIPDDDFALESLFTS 77
           + I+ FE +++ A+ VQ  TL++IL+ N+  EYL+ + LG     E           + S
Sbjct: 8   ETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAE----------SYKS 67

Query: 78  LVPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLL 137
            +PL  H D+EPY+QRI DGD++P++T +PI  LSLSSGTT G+ K++PF     +TTL 
Sbjct: 68  CIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQ 127

Query: 138 IFRLAAAYRSRQ----------------------------------------------QE 197
           I+R + A+R+R+                                              Q 
Sbjct: 128 IYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQS 187

Query: 198 DCSSYDAI----------------------------------------------DD---- 257
            C S D +                                              +D    
Sbjct: 188 QCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCTD 247

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 248 IRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIGLSNWYGVIPALWPNAKYVYGI 307

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 308 MTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYFEFI 367

[Close]

BLAST of Sgr023622 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 236.5 bits (602), Expect = 4.8e-61
Identity = 167/573 (29.14%), Postives = 245/573 (42.76%), Query Frame = 0


Query: 19  IIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSLV 78
           +ID F++++++A  VQ +TL++IL +N    YL+    + N  +        E  F S+V
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATD-------PEEAFKSMV 72

Query: 79  PLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLIF 138
           PL +  +LEPY++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +F
Sbjct: 73  PLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLF 132

Query: 139 RLAAAYRSRQ------------------------------------------------QE 198
           R A A+R+R                                                   
Sbjct: 133 RTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSP 192

Query: 199 DCSSYDAI---------------------------------------------------- 258
            CS  + I                                                    
Sbjct: 193 SCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDI 252

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 253 KDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYGIM 312

Query: 319 -------------------------------------------------------DDFL- 367
                                                                   +FL 
Sbjct: 313 TGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLP 372

[Close]

BLAST of Sgr023622 vs. ExPASy TrEMBL
Match: A0A6J1J741 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC111484069 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.9e-141
Identity = 302/589 (51.27%), Postives = 324/589 (55.01%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSL 77
           D++DWFE+VS  A + QSRTLR+IL QN GVEYL +WLGDVNIH+IP DDFALESLFTSL
Sbjct: 7   DMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIP-DDFALESLFTSL 66

Query: 78  VPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 137
           VPLSSHAD EP+LQRIADGDS+PLLTQQPI+TLSLSSGTTEGRQKYVPFT HSAQTTLLI
Sbjct: 67  VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 126

Query: 138 FRLAAAYRSR-------------------------------------------------- 197
           FR+AAAYRSR                                                  
Sbjct: 127 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 186

Query: 198 ------------------------------------------------------------ 257
                                                                       
Sbjct: 187 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 246

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 247 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCKYVY 306

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 307 SIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYFE 366

[Close]

BLAST of Sgr023622 vs. ExPASy TrEMBL
Match: A0A6J1FRQ1 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LOC111448207 PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.8e-140
Identity = 301/589 (51.10%), Postives = 322/589 (54.67%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSL 77
           D++DWFE+VS  A + Q RTLR+IL QN GVEYL +WLGDVNIH+IP DDFALESLFTSL
Sbjct: 7   DMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIP-DDFALESLFTSL 66

Query: 78  VPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 137
           +PLSSHAD EP+LQRIADGDS+PLLTQQPI+TLSLSSGTTEGRQKYVPFT HSAQTTLLI
Sbjct: 67  IPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 126

Query: 138 FRLAAAYRSR-------------------------------------------------- 197
           FR+AAAYRSR                                                  
Sbjct: 127 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 186

Query: 198 ------------------------------------------------------------ 257
                                                                       
Sbjct: 187 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 246

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 247 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 306

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 307 SIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 366

[Close]

BLAST of Sgr023622 vs. ExPASy TrEMBL
Match: A0A6J1CHT7 (indole-3-acetic acid-amido synthetase GH3.10 OS=Momordica charantia OX=3673 GN=LOC111011043 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 4.7e-136
Identity = 304/614 (49.51%), Postives = 327/614 (53.26%), Query Frame = 0


Query: 2   EAPQGLGVTNDH----LTEHDIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGD 61
           EA  G G  NDH      E +IIDWFEQV+  A + QSRTLRQIL QN GVEYLK WLGD
Sbjct: 4   EAEAG-GAGNDHGELSEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGD 63

Query: 62  VNIHEIPDDDFALESLFTSLVPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTT 121
           VNIH+I DDD ALESLFTS VPLSSH   EP+LQRIADGDS PLLTQ PIKTLSLSSGTT
Sbjct: 64  VNIHQIQDDDSALESLFTSSVPLSSHPHFEPFLQRIADGDSTPLLTQLPIKTLSLSSGTT 123

Query: 122 EGRQKYVPFTRHSAQTTLLIFRLAAAYRSR------------------------------ 181
           EGRQKYV FT HSAQTTLLIFRLAAAYRSR                              
Sbjct: 124 EGRQKYVLFTPHSAQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGT 183

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 184 ATTHYYASEEFKIKQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFA 243

Query: 242 ------------------------------------------------------------ 301
                                                                       
Sbjct: 244 YTILQAFNQLEEVWPDICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRL 303

Query: 302 ------------------------------------------------------------ 361
                                                                       
Sbjct: 304 RARDWFGLIPELWPNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVD 363

Query: 362 ------------------------------QQEDCSS-YD-AIDDFLEGEPVPLSQVKIG 368
                                         Q + CSS +D  +DDFLEGEPVPLS+VKIG
Sbjct: 364 PSLPPENVTFAVVPTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIG 423

[Close]

BLAST of Sgr023622 vs. ExPASy TrEMBL
Match: A0A5D3B9T4 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00300 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 7.0e-132
Identity = 288/589 (48.90%), Postives = 315/589 (53.48%), Query Frame = 0


Query: 16  EHDIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFT 75
           E DIIDWFEQVS  A++ QSRTLR+IL QN GVEYLKKW+GDVNIHEIP DDF L+S+FT
Sbjct: 10  EEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIP-DDFTLQSIFT 69

Query: 76  SLVPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTL 135
           SL+PLSSHA  EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFT HSA TTL
Sbjct: 70  SLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTTL 129

Query: 136 LIFRLAAAYRSR------------------------------------------------ 195
           LIFRLAAAYRSR                                                
Sbjct: 130 LIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKT 189

Query: 196 ------------------------------------------------------------ 255
                                                                       
Sbjct: 190 KSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEII 249

Query: 256 ------------------------------------------------------------ 315
                                                                       
Sbjct: 250 HDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPKLWPNCKY 309

Query: 316 ------------------------------------------------------------ 368
                                                                       
Sbjct: 310 VYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSY 369

[Close]

BLAST of Sgr023622 vs. ExPASy TrEMBL
Match: A0A0A0KE57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 7.0e-132
Identity = 289/589 (49.07%), Postives = 313/589 (53.14%), Query Frame = 0


Query: 16  EHDIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFT 75
           E DII WFEQVS  A+L QSRTLR+IL QN GVEYLKKW+GDVNIHEIP DDF L S+FT
Sbjct: 4   EEDIIGWFEQVSSEAALAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIP-DDFTLHSIFT 63

Query: 76  SLVPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTL 135
           S +PLSSHA  EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFT HSAQTTL
Sbjct: 64  SSIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAQTTL 123

Query: 136 LIFRLAAAYRSR------------------------------------------------ 195
           LIFRLAAAYRSR                                                
Sbjct: 124 LIFRLAAAYRSRVYPIRDGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKT 183

Query: 196 ------------------------------------------------------------ 255
                                                                       
Sbjct: 184 KSFTCSPQEVITGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEMI 243

Query: 256 ------------------------------------------------------------ 315
                                                                       
Sbjct: 244 HDLSHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELMKEGWLGLIPKLWPNCKY 303

Query: 316 ------------------------------------------------------------ 368
                                                                       
Sbjct: 304 VYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSY 363

[Close]

BLAST of Sgr023622 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.5e-113
Identity = 247/585 (42.22%), Postives = 296/585 (50.60%), Query Frame = 0


Query: 18  DIIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSL 77
           D+I WFE VS++A  VQS TLR+IL+ NSGVEYL+KWLG V++ ++  DD+ LE+LFTSL
Sbjct: 10  DVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM--DDYTLETLFTSL 69

Query: 78  VPLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 137
           VP+ SHADL+PY+QRIADG+++PLLTQ+PI  LSLSSGTTEGRQKYVPFTRHSAQTTL I
Sbjct: 70  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 129

Query: 138 FRLAAAYRSR-------------------------------------------QQE---- 197
           FRL+AAYRSR                                           +QE    
Sbjct: 130 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 189

Query: 198 -DCSSYDAID-------------------------------------------------- 257
             CS  + I                                                   
Sbjct: 190 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 249

Query: 258 ------------------------------------------------------------ 317
                                                                       
Sbjct: 250 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 309

Query: 318 ------------------------------------------------------------ 367
                                                                       
Sbjct: 310 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 369

[Close]

BLAST of Sgr023622 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 236.5 bits (602), Expect = 3.4e-62
Identity = 167/573 (29.14%), Postives = 245/573 (42.76%), Query Frame = 0


Query: 19  IIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSLV 78
           +ID F++++++A  VQ +TL++IL +N    YL+    + N  +        E  F S+V
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATD-------PEEAFKSMV 72

Query: 79  PLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLIF 138
           PL +  +LEPY++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +F
Sbjct: 73  PLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLF 132

Query: 139 RLAAAYRSRQ------------------------------------------------QE 198
           R A A+R+R                                                   
Sbjct: 133 RTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSP 192

Query: 199 DCSSYDAI---------------------------------------------------- 258
            CS  + I                                                    
Sbjct: 193 SCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDI 252

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 253 KDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYGIM 312

Query: 319 -------------------------------------------------------DDFL- 367
                                                                   +FL 
Sbjct: 313 TGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLP 372

[Close]

BLAST of Sgr023622 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 236.5 bits (602), Expect = 3.4e-62
Identity = 167/573 (29.14%), Postives = 245/573 (42.76%), Query Frame = 0


Query: 19  IIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSLV 78
           +ID F++++++A  VQ +TL++IL +N    YL+    + N  +        E  F S+V
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATD-------PEEAFKSMV 72

Query: 79  PLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLIF 138
           PL +  +LEPY++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +F
Sbjct: 73  PLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLF 132

Query: 139 RLAAAYRSRQ------------------------------------------------QE 198
           R A A+R+R                                                   
Sbjct: 133 RTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSP 192

Query: 199 DCSSYDAI---------------------------------------------------- 258
            CS  + I                                                    
Sbjct: 193 SCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDI 252

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 253 KDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYGIM 312

Query: 319 -------------------------------------------------------DDFL- 367
                                                                   +FL 
Sbjct: 313 TGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLP 372

[Close]

BLAST of Sgr023622 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 236.5 bits (602), Expect = 3.4e-62
Identity = 167/573 (29.14%), Postives = 245/573 (42.76%), Query Frame = 0


Query: 19  IIDWFEQVSKHASLVQSRTLRQILDQNSGVEYLKKWLGDVNIHEIPDDDFALESLFTSLV 78
           +ID F++++++A  VQ +TL++IL +N    YL+    + N  +        E  F S+V
Sbjct: 24  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATD-------PEEAFKSMV 83

Query: 79  PLSSHADLEPYLQRIADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLIF 138
           PL +  +LEPY++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +F
Sbjct: 84  PLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLF 143

Query: 139 RLAAAYRSRQ------------------------------------------------QE 198
           R A A+R+R                                                   
Sbjct: 144 RTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSP 203

Query: 199 DCSSYDAI---------------------------------------------------- 258
            CS  + I                                                    
Sbjct: 204 SCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDI 263

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 264 KDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYGIM 323

Query: 319 -------------------------------------------------------DDFL- 367
                                                                   +FL 
Sbjct: 324 TGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLP 383

[Close]

BLAST of Sgr023622 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 226.5 bits (576), Expect = 3.5e-59
Identity = 108/206 (52.43%), Postives = 152/206 (73.79%), Query Frame = 0


Query: 163 EGEPVPLSQVKIGQEYEVVLTTFTGLYRCRLGDVVEVADFHNKTPKLNFICRRKLVLTVN 222
           E +PV L+QVKIG+EYEVV+T + GLYR RLGDVV+V  F+N TP+L FICRR L+L++N
Sbjct: 294 EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSIN 353

Query: 223 IDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHANLADRGQPGHYVIFWEVKGDVEDRV 282
           IDKNTE+DLQL VE  ++ L+++   E++DF+S+ +++    PGHY IFWE+ G+  + V
Sbjct: 354 IDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVST--DPGHYAIFWEISGETNEDV 413

Query: 283 LGECCREMDAAFVDYGYVVSRRSNSIGPLELRIVERGTFKKILEHYIGNGAALSQFKTPR 342
           L +CC  +D AF+D GYV SR+  +IG LELR+V +GTF+KI EH++G G++  QFK PR
Sbjct: 414 LQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPR 473

Query: 343 CT--NNQVLLTILNLSTVKRFRSTAY 367
           C   +N  +L IL  + V  + STA+
Sbjct: 474 CVKPSNAKVLQILCENVVSSYFSTAF 496

HSP 2 Score: 62.8 bits (151), Expect = 6.7e-10
Identity = 27/55 (49.09%), Postives = 41/55 (74.55%), Query Frame = 0


Query: 93  IADGDSAPLLTQQPIKTLSLSSGTTEGRQKYVPFTRHSAQTTLLIFRLAAAYRSR 148
           + DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +FR A A+R+R
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNR 55

The following BLAST results are available for this feature:

BLAST of Sgr023622 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986272.1	1.0e-140	51.27	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima]	[more]
XP_022943441.1	3.8e-140	51.10	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata]	[more]
XP_023512179.1	3.8e-140	51.27	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo]	[more]
KAG6570848.1	5.0e-140	51.10	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
KAG7010696.1	8.0e-138	49.67	Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argy...	[more]

BLAST of Sgr023622 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNS2	2.1e-112	42.22	Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
A0A1J6KGJ9	6.0e-72	31.01	Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ...	[more]
A0A314KSQ4	3.0e-71	31.83	Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ...	[more]
Q6I581	8.7e-63	29.57	Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9SKE2	4.8e-61	29.14	Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ...	[more]

BLAST of Sgr023622 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J741	4.9e-141	51.27	indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1FRQ1	1.8e-140	51.10	indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1CHT7	4.7e-136	49.51	indole-3-acetic acid-amido synthetase GH3.10 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A5D3B9T4	7.0e-132	48.90	Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A0A0KE57	7.0e-132	49.07	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=3 SV=1	[more]

BLAST of Sgr023622 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03400.1	1.5e-113	42.22	Auxin-responsive GH3 family protein	[more]
AT2G46370.1	3.4e-62	29.14	Auxin-responsive GH3 family protein	[more]
AT2G46370.2	3.4e-62	29.14	Auxin-responsive GH3 family protein	[more]
AT2G46370.4	3.4e-62	29.14	Auxin-responsive GH3 family protein	[more]
AT2G46370.3	3.5e-59	52.43	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 22..149 e-value: 6.4E-30 score: 104.5 coord: 164..347 e-value: 7.7E-65 score: 219.7
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 154..366
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 13..147
None	No IPR available	PANTHER	PTHR31901:SF41	JASMONIC ACID-AMIDO SYNTHETASE JAR1-LIKE	coord: 154..366
None	No IPR available	PANTHER	PTHR31901:SF41	JASMONIC ACID-AMIDO SYNTHETASE JAR1-LIKE	coord: 13..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr023622.1	Sgr023622.1	mRNA