Sgr022292 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr022292 vs. NCBI nr
Match: XP_022154635.1 (U-box domain-containing protein 34-like [Momordica charantia])

HSP 1 Score: 929.5 bits (2401), Expect = 3.2e-266
Identity = 531/736 (72.15%), Postives = 558/736 (75.82%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVAVNG RG  GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV
Sbjct: 1   MTSVAVAVNGGRG--GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 120
           SELDADVVALYVHDVKEKYEQVF PFKKLC REKFLLLVETLVLEDDNPATALLRY SES
Sbjct: 61  SELDADVVALYVHDVKEKYEQVFAPFKKLCRREKFLLLVETLVLEDDNPATALLRYVSES 120

Query: 121 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 180
           GIKRLVLGSCFR+CI RKL GPSVPS+ILRAAPASFDIYVIYK RVITR T+  TAPST 
Sbjct: 121 GIKRLVLGSCFRSCIARKLKGPSVPSAILRAAPASFDIYVIYKGRVITRPTA--TAPST- 180

Query: 181 QTNSRQWMLGDTDYYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQLNSLSTLTE 240
           +T+SRQWMLGDTDYYRGS  ISEQSS TFSSSS SNVH+RGDSLE NSTEQLNSLSTLTE
Sbjct: 181 ETDSRQWMLGDTDYYRGSGAISEQSSATFSSSSVSNVHQRGDSLEGNSTEQLNSLSTLTE 240

Query: 241 EEDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQAL 300
           EEDMRAEVESLQLELETTVSLYK+ CEELVHAQK+VQSLTSECLEESRRV+ ALEREQAL
Sbjct: 241 EEDMRAEVESLQLELETTVSLYKRACEELVHAQKQVQSLTSECLEESRRVSEALEREQAL 300

Query: 301 RKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRY 360
           R VA+EEKAKHLEAMKELE AKDLLAK+AYERQLA+LNALKES+EKQKIVDTLLTNDRRY
Sbjct: 301 RTVASEEKAKHLEAMKELEEAKDLLAKEAYERQLADLNALKESVEKQKIVDTLLTNDRRY 360

Query: 361 RRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVE 420
           RRYT AEIEAATNFFE VNVIGEGGYGKVY+CSLDHTPVA+KVLQHDVLEKKDEFLKEVE
Sbjct: 361 RRYTPAEIEAATNFFEQVNVIGEGGYGKVYRCSLDHTPVAIKVLQHDVLEKKDEFLKEVE 420

Query: 421 ILSQIRHPHVVLLLGACPER---------------------------------------- 480
           ILSQIRHPHVVLLLGACPE+                                        
Sbjct: 421 ILSQIRHPHVVLLLGACPEQGCLIYEYMENGSLDDHILLRNGKTPLPWFVRFRIAFQVAS 480

Query: 481 ----------------------------------DVGMAKIIGDIVPDNVTEYRNTIIAG 540
                                             DVGMAKIIGDIVPDNVTEYRNTI+AG
Sbjct: 481 GLAFLHNSNPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAG 540

Query: 541 TLHYLDPEYQRTGTLRPKSDT--------------------------------------S 600
           TLHY+DPEYQRTGTLRPKSDT                                      S
Sbjct: 541 TLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVESSIASSSLGDILDRS 600

Query: 601 ITDWPLAEAEELARLALKCLKLRCRDRPDLETEVLPVLKSLVDFADACQMKNK------- 609
           ITDWPLAEAE LARLALKCLKLRCRDRPDL+TEVLPVLK LVDFADACQ + K       
Sbjct: 601 ITDWPLAEAEVLARLALKCLKLRCRDRPDLDTEVLPVLKRLVDFADACQNEEKCPANPPS 660

BLAST of Sgr022292 vs. NCBI nr
Match: XP_022999433.1 (U-box domain-containing protein 34-like [Cucurbita maxima])

HSP 1 Score: 910.6 bits (2352), Expect = 1.5e-260
Identity = 505/697 (72.45%), Postives = 539/697 (77.33%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGG+GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+F+PFKK+C REKFLLLVETL+LEDDNPATAL+RYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALMRYASES 120

Query: 121 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 180
           GIK LVLGSCFR CI R+L G SVPS+I+RAA   FDIYVIY+RRVITRT  ATTAPS+ 
Sbjct: 121 GIKHLVLGSCFRTCIARRLKGDSVPSAIMRAASRCFDIYVIYRRRVITRT--ATTAPSS- 180

Query: 181 QTNSRQWMLGDTDYYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQLNSLSTLTE 240
           +T+SRQWMLGDTDYYRGSS +SE+S GT SSSS SNVHRRGDSLE NSTEQLNSLSTLTE
Sbjct: 181 ETDSRQWMLGDTDYYRGSSTVSEKSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTE 240

Query: 241 EEDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQAL 300
           EEDM+AEVESLQLELE+TVSLYKQ CEELVHAQKKVQSLTSECLEESR+VNAALEREQAL
Sbjct: 241 EEDMQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQAL 300

Query: 301 RKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRY 360
           RKVAAEEKAKHLEA+KELE AKD LAK+AYERQLAELNALKESLEK+KIVDTLLTNDRRY
Sbjct: 301 RKVAAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRY 360

Query: 361 RRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVE 420
           RRYTTAEIEAATNFF+ VNVIGEGGYGKVY CSLDHTPVA+KV +HD+ E KDEFLKEVE
Sbjct: 361 RRYTTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVE 420

Query: 421 ILSQIRHPHVVLLLGACPER---------------------------------------- 480
           ILSQIRHPHVVLLLGACPER                                        
Sbjct: 421 ILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRLRIVFQVAS 480

Query: 481 ----------------------------------DVGMAKIIGDIVPDNVTEYRNTIIAG 540
                                             DVGMAKIIGDIVPDNVTEYRNTI+AG
Sbjct: 481 GLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAG 540

Query: 541 TLHYLDPEYQRTGTLRPKSDT--------------------------------------S 573
           TLHY+DPEYQRTGTLRPKSDT                                      S
Sbjct: 541 TLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVENSIASGSLADILDKS 600

BLAST of Sgr022292 vs. NCBI nr
Match: XP_023546893.1 (U-box domain-containing protein 34-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 907.5 bits (2344), Expect = 1.3e-259
Identity = 502/696 (72.13%), Postives = 537/696 (77.16%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGG+GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 21  MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 80

Query: 61  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+F+PFKK+C REKFLLLVETL+LEDDNPATAL+RYASES
Sbjct: 81  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALVRYASES 140

Query: 121 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 180
           GIK LVLGSCFR CI R+L G SVPS+I+RAA   FDIYVIY+RRVITRT  ATTAPS+ 
Sbjct: 141 GIKHLVLGSCFRTCIARRLKGDSVPSAIMRAASRCFDIYVIYRRRVITRT--ATTAPSS- 200

Query: 181 QTNSRQWMLGDTDYYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQLNSLSTLTE 240
           +T+SRQWMLGDTDYYRGSS +SE+S GT SSSS SNVHRRGDSLE NSTEQLNSLSTLTE
Sbjct: 201 ETDSRQWMLGDTDYYRGSSTVSERSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTE 260

Query: 241 EEDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQAL 300
           EEDM+AEVESLQLELE+TVSLYKQ CEELVHAQKKVQSLTSECLEESR+VNAALEREQAL
Sbjct: 261 EEDMQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQAL 320

Query: 301 RKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRY 360
           RKVAAEEKAKHLEA+KELE AKD LAK+AYERQLAELNALKESLEK+KIVDTLLTNDRRY
Sbjct: 321 RKVAAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRY 380

Query: 361 RRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVE 420
           RRYTTAEIEAATNFF+ VNVIGEGGYGKVY CSLDHTPVA+KV +HD+ E KDEFLKEVE
Sbjct: 381 RRYTTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVE 440

Query: 421 ILSQIRHPHVVLLLGACPER---------------------------------------- 480
           ILSQIRHPHVVLLLGACPER                                        
Sbjct: 441 ILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRFRIVFQVAS 500

Query: 481 ----------------------------------DVGMAKIIGDIVPDNVTEYRNTIIAG 540
                                             DVGMAKIIGDIVPDNVTEYRNTI+AG
Sbjct: 501 GLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAG 560

Query: 541 TLHYLDPEYQRTGTLRPKSDT--------------------------------------S 573
           TLHY+DPEYQRTGTLRPKSDT                                      S
Sbjct: 561 TLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVENSIASTSLADILDKS 620

BLAST of Sgr022292 vs. NCBI nr
Match: KAG6599517.1 (U-box domain-containing protein 34, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 907.1 bits (2343), Expect = 1.7e-259
Identity = 502/696 (72.13%), Postives = 536/696 (77.01%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGG+GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+F+PFKK+C REKFLLLVETL+LEDDNPATAL+RYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALMRYASES 120

Query: 121 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 180
           GIK LVLGSCFR CI R+L G SVP +I+RAA   FDIYVIY+RRVITRT  ATTAPS+ 
Sbjct: 121 GIKHLVLGSCFRTCIARRLKGDSVPFAIMRAASRCFDIYVIYRRRVITRT--ATTAPSS- 180

Query: 181 QTNSRQWMLGDTDYYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQLNSLSTLTE 240
           +T+SRQWMLGDTDYYRGSS +SE+S GT SSSS SNVHRRGDSLE NSTEQLNSLSTLTE
Sbjct: 181 ETDSRQWMLGDTDYYRGSSTVSEKSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTE 240

Query: 241 EEDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQAL 300
           EEDM+AEVESLQLELE+TVSLYKQ CEELVHAQKKVQSLTSECLEESR+VNAALEREQAL
Sbjct: 241 EEDMQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQAL 300

Query: 301 RKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRY 360
           RKVAAEEKAKHLEA+KELE AKD LAK+AYERQLAELNALKESLEK+KIVDTLLTNDRRY
Sbjct: 301 RKVAAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRY 360

Query: 361 RRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVE 420
           RRYTTAEIEAATNFF+ VNVIGEGGYGKVY CSLDHTPVA+KV +HD+ E KDEFLKEVE
Sbjct: 361 RRYTTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVE 420

Query: 421 ILSQIRHPHVVLLLGACPER---------------------------------------- 480
           ILSQIRHPHVVLLLGACPER                                        
Sbjct: 421 ILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRFRIVFQVAS 480

Query: 481 ----------------------------------DVGMAKIIGDIVPDNVTEYRNTIIAG 540
                                             DVGMAKIIGDIVPDNVTEYRNTI+AG
Sbjct: 481 GLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAG 540

Query: 541 TLHYLDPEYQRTGTLRPKSDT--------------------------------------S 573
           TLHY+DPEYQRTGTLRPKSDT                                      S
Sbjct: 541 TLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVENSIASASLADILDKS 600

BLAST of Sgr022292 vs. NCBI nr
Match: XP_038891123.1 (U-box domain-containing protein 34-like [Benincasa hispida])

HSP 1 Score: 907.1 bits (2343), Expect = 1.7e-259
Identity = 502/699 (71.82%), Postives = 537/699 (76.82%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           M SVAVAVNGVRGG+GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MISVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQVF+PFKKLC REKFLLLVETLVLEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLVLEDDNPATALLRYASES 120

Query: 121 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 180
           GIKRLVLGSCFR C+ RKL G SVPS+I+R   +SFDI+VIYKRRVITR   A+TAPS  
Sbjct: 121 GIKRLVLGSCFRTCVARKLKGDSVPSAIMRTVHSSFDIHVIYKRRVITR--KASTAPS-N 180

Query: 181 QTNSRQWMLGDTDYYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQLNSLSTLTE 240
           +T+SRQWMLGDTDYYRGSS  SE+S GTFSSSS S  HRRGDSL+ NSTE+LNSL+TLTE
Sbjct: 181 ETDSRQWMLGDTDYYRGSSAASEKSLGTFSSSSLSIGHRRGDSLKVNSTEELNSLNTLTE 240

Query: 241 EEDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQAL 300
           EEDM+AEVESLQLELETTVSLYKQ CEELVHAQKKVQSLTSECLEESRRVNAALEREQAL
Sbjct: 241 EEDMQAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREQAL 300

Query: 301 RKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRY 360
           RK  A+EK KHLEA+KELE AKDLLAK+AYERQLAELNALKES+EKQKIVDTLLTNDRRY
Sbjct: 301 RKTVAKEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESVEKQKIVDTLLTNDRRY 360

Query: 361 RRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVE 420
           RRYTTAEIEAATNFF+ VNVIGEGGYGKVY CSLDHTPVA+KV QHD+ +KKDEFLKEVE
Sbjct: 361 RRYTTAEIEAATNFFDEVNVIGEGGYGKVYNCSLDHTPVAIKVFQHDIFKKKDEFLKEVE 420

Query: 421 ILSQIRHPHVVLLLGACPER---------------------------------------- 480
           ILSQIRHPHVVLLLGACPER                                        
Sbjct: 421 ILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVAS 480

Query: 481 ----------------------------------DVGMAKIIGDIVPDNVTEYRNTIIAG 540
                                             DVGMAKIIGDIVPDNVTEYRNTI+AG
Sbjct: 481 GLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAG 540

Query: 541 TLHYLDPEYQRTGTLRPKSDT--------------------------------------S 573
           TLHY+DPEYQRTGTLRPKSDT                                      S
Sbjct: 541 TLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKS 600

BLAST of Sgr022292 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 3.2e-120
Identity = 299/781 (38.28%), Postives = 393/781 (50.32%), Query Frame = 0

Query: 4   VAVAVNGVRGGR-GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTP--------- 63
           VAVAV G+ G + GG GSRRAVRWAV+NLLP AD+F+++HV+P ITSIPTP         
Sbjct: 24  VAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKADKFVMIHVIPTITSIPTPNILILMFTR 83

Query: 64  -----MGDRVAVSELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDN 123
                 GDR+ V E++  VV +YV DVK++YE VF+PF K+C                  
Sbjct: 84  MWVVTAGDRLPVEEVEESVVEMYVRDVKKEYETVFVPFLKMCK----------------- 143

Query: 124 PATALLRYASESGIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVIT 183
                    S    KR      FR   +R+  G  VP ++LR AP + ++Y++ K R+ T
Sbjct: 144 ---------STRSTKRY-----FR---SRRTKGTGVPLTVLRYAPETCEVYIVCKDRITT 203

Query: 184 RT---------TSATTAPSTGQTNSRQWMLGDTDYYRGSSGISEQS--SGTFSSSSRSNV 243
           ++          ++  A +T     R W          +     QS  +GT  S+S   +
Sbjct: 204 KSMDPLINREPCTSPHAAATAHDFLRDWAASFHTLRSPTLPDPRQSTEAGTRRSASAREL 263

Query: 244 HRRGDSLEAN--STEQLNSLSTLTEEE--------------------------------- 303
                SL  N   T Q +  S+ T  E                                 
Sbjct: 264 RFEALSLTCNKPKTPQSSKASSATTPEIFRRRRGSDIPQLNYSDFDKTCTKPQSNVENIV 323

Query: 304 --------------------DMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSE 363
                               ++  EVE L+ EL++TV  YKQ CEEL   Q KV+ L++E
Sbjct: 324 SEHRDSDRSPPETSRKSKKVEIEEEVERLKNELQSTVFKYKQACEELFSTQNKVKMLSTE 383

Query: 364 CLEESRRVNAALEREQALRKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKE 423
            L ES+RVN A+E+E+  R  AA EK ++++A+KE+E AK LLA++  +RQ+AE+NAL+ 
Sbjct: 384 YLNESKRVNNAVEKEELQRNTAALEKERYMKAVKEVETAKALLAREFCQRQIAEVNALRT 443

Query: 424 SLEKQKIVDTLLTNDRRYRRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVK 483
            LEK+K++D LL  D RYR+YT  EI  AT  F P  VIGEGGYGKVY+CSLD TP AVK
Sbjct: 444 YLEKKKVIDQLLGTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLDSTPAAVK 503

Query: 484 VLQHDVLEKKDEFLKEVEILSQIRHPHVVLLLGACPER---------------------- 543
           V++ D  EKK EFLKEVE+LSQ+RHPHVVLLLGACPE                       
Sbjct: 504 VVRLDTPEKKQEFLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGSLEEYIFHRKN 563

Query: 544 ----------------------------------------------------DVGMAKII 577
                                                               DVG+AK++
Sbjct: 564 KPPLPWFIRFRVIFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSKIADVGLAKLV 623

BLAST of Sgr022292 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 7.4e-64
Identity = 227/805 (28.20%), Postives = 341/805 (42.36%), Query Frame = 0

Query: 3   SVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTA-DRFILVHVMPKITSIPTPMGDRVAVS 62
           SVAVA+NG +       S+  V WA+E  +P     F L++V P ++ IPTPMG  VAVS
Sbjct: 23  SVAVAINGKK------KSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVS 82

Query: 63  ELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASESG 122
           EL  DVV+ Y  ++     ++  P+KK+  R K  + VE L+L+   PA A+    + +G
Sbjct: 83  ELREDVVSAYKQELDWSANEMLRPYKKMFERRK--VQVEVLLLDSLEPAAAIAEEIAGTG 142

Query: 123 IKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTGQ 182
           + +LV+G   R   +RK+    + S I  A P    +YVI K ++ +   S + A  + +
Sbjct: 143 VTKLVIGMSLRGFFSRKI---DMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIR 202

Query: 183 TNSRQWMLGDTDYYR------------------------------GSSGISE---QSSGT 242
                   G TD  R                              GS+ +++    SSGT
Sbjct: 203 FERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGT 262

Query: 243 --------------------------------------------------------FSSS 302
                                                                     SS
Sbjct: 263 DQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSS 322

Query: 303 SRSNVHRRGD---------------SLEANSTEQLNSLSTLTEEE-DMRAEVESLQLELE 362
           S SN H   +                  ++ ++ L S+ ++T+ + ++  E+E L+ EL+
Sbjct: 323 SSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELK 382

Query: 363 TTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQALRKVAAEEKAKHLEAMK 422
               +Y     E V A KK+  L     EES ++    E+E+  +  A++EK ++ EAMK
Sbjct: 383 HVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMK 442

Query: 423 ELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRYTTAEIEAATNFFE 482
           E E  K+L+ K+A  R+ AE  A +++ EK K+  +L++   +Y+ YT  EI AAT+ F 
Sbjct: 443 EAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFA 502

Query: 483 PVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVEILSQIRHPHVVLLLGA 542
               IG G YG VYKC+L HT  AVKVL     +   +F +E+EILS+IRHPH+VLLLGA
Sbjct: 503 ENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGA 562

Query: 543 CPER-------------------------------------------------------- 573
           CPER                                                        
Sbjct: 563 CPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHR 622

BLAST of Sgr022292 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 230.7 bits (587), Expect = 9.3e-59
Identity = 215/814 (26.41%), Postives = 341/814 (41.89%), Query Frame = 0

Query: 16  GGGGSRRAVRWAVENLLPTAD-RFILVHVMPKITSIPTPMGDRVAVSELDADVVALYVHD 75
           G   S+  V WA+E      +  F L+H+ P ITS+PTPMG+ + +SE+  DVV  Y  +
Sbjct: 28  GSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQE 87

Query: 76  VKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASESGIKRLVLGSCFRNC 135
           +  + E++  P+ KL  R K  + VE LV+E DN A A+    +   I R+V+G   R+ 
Sbjct: 88  ILWQSEEMLKPYTKLFVRRK--VAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSF 147

Query: 136 ITRKLIGPSVPSSILRAAPASFDIYVIYKRRVI--------------------TRTTSAT 195
            +RK    SV S+++   P    +YV+ K ++                     T ++S +
Sbjct: 148 FSRKADICSVISALM---PNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSERTNSSSGS 207

Query: 196 TAPST-------------------------------GQ-----------TNSRQWMLGDT 255
           + P++                               GQ           ++  + M  D 
Sbjct: 208 SGPTSDSSDVMSSAHDSQSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSLDA 267

Query: 256 DYYRGSSGISEQSSGTFS----------------SSSRSN-------------------V 315
           +  R  S I+  S+ T S                SSS SN                    
Sbjct: 268 EEARDVSSINRSSTDTTSRWTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTT 327

Query: 316 HRRGDSLEANSTEQLNSLSTLTEEEDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQ 375
           H R     +N ++ L+  S    + ++  EVE L+ EL     +Y     E   A +K+ 
Sbjct: 328 HSRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLG 387

Query: 376 SLTSECLEESRRVNAALEREQALRKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAEL 435
            L    LEE+ ++     +E   R++A +EK    +A ++ E+ ++   ++  +R+ AE 
Sbjct: 388 ELNQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAER 447

Query: 436 NALKESLEKQKIVDTLLTNDRRYRRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHT 495
            + +++ EK+K+  TL +   +Y+ +   EI AAT+ F     IG G YG VYKC+L HT
Sbjct: 448 KSARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHT 507

Query: 496 PVAVKVLQHDVLEKKDEFLKEVEILSQIRHPHVVLLLGACPER----------------- 555
              VKVLQ    +   +F +E+EILS+IRHPH+VLLLGACPE+                 
Sbjct: 508 TAVVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRL 567

Query: 556 ---------------------------------------------------------DVG 602
                                                                    DVG
Sbjct: 568 FQVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVG 627

BLAST of Sgr022292 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 1.5e-48
Identity = 203/778 (26.09%), Postives = 311/778 (39.97%), Query Frame = 0

Query: 4   VAVAVNGVRGGRGGGGSRRAVRWAVENLLPTAD-RFILVHVMPKITSIPTPMGDRVAVSE 63
           VAVA+ G         ++  VRWA++         F L+HV P+         D  +VS 
Sbjct: 8   VAVAIKG-----NNSKTKGVVRWALQEFASQEHVVFKLLHVQPR---------DSNSVST 67

Query: 64  LDADV-VALYVHDVKEKYEQVFIPFKKLCSREKFL---LLVETLVLEDDNPATALLRYAS 123
              D+  ++Y  DV  K  ++ +P     SR+ F+   + ++ +VLE D+ A A+ +   
Sbjct: 68  TRKDLTTSVYKKDVDRKTREMLLP-----SRDMFVHREVQLDIMVLESDDIADAISKAVQ 127

Query: 124 ESGIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVI-TRTTSATTAP 183
           + GI  LV+G+      + KL   ++ S I  A P    ++VI K +++  R +   T  
Sbjct: 128 DHGISELVIGASSSIIFSWKLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTET 187

Query: 184 STGQTNSRQWMLGDTDYYRGSSGISEQSS-----------------------GTFSSSSR 243
           S     S      D+     SS  S Q S                       G  ++   
Sbjct: 188 SIADDRSESRFSSDSHSGTVSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIGKQNNEPH 247

Query: 244 SNVHRRGDSLEANSTEQLN-------------------------------SLSTLTEEED 303
            + H R  SL+ + ++ LN                               S ST ++   
Sbjct: 248 HHHHNRAGSLDVDESKLLNQKGFYRTSSSGIGYGGSDISSWRSSQMEEASSSSTYSDPTS 307

Query: 304 MRA------EVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALERE 363
             +      E+E L++EL     +Y     E++ A KK+Q L     EE+ R+     RE
Sbjct: 308 SSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKNLTIRE 367

Query: 364 QALRKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTL---L 423
           +   +V   E+ +  +A  E E  ++ + ++  ER  AE  A +   EKQ++ D L    
Sbjct: 368 EEADEVVEMERERQEDAENEAELVRECIERETEERLEAEARAEEVRKEKQRLEDALEGGP 427

Query: 424 TNDRRYRRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDE 483
              ++Y ++   EI  AT+ F     IG GGYG VY+C+L HT VAVKVL  D      +
Sbjct: 428 LQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSSLTKQ 487

Query: 484 FLKEVEILSQIRHPHVVLLLGACPER---------------------------------- 543
           F +E+EILS+IRHPH++LLLGACPER                                  
Sbjct: 488 FHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPPPLRW 547

Query: 544 ----------------------------------------------DVGMAKIIGDIVPD 573
                                                         DVG++K++      
Sbjct: 548 FERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNLDPSH 607

BLAST of Sgr022292 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 3.1e-46
Identity = 216/812 (26.60%), Postives = 330/812 (40.64%), Query Frame = 0

Query: 3   SVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTAD-RFILVHVMPKITSIPTPMGDRVAVS 62
           +VA+A++      G   S+  ++WA+       +  F L+H+ PKIT++PT  G+ V++S
Sbjct: 33  TVALAIS------GSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVSIS 92

Query: 63  ELDADVVALYVHDV-KEKYEQVFIPFKKLCSREKFLL------------LVETLVLEDDN 122
           E   +V A Y   V +E  E +  PFKK+C R+K  +             VE  VLE ++
Sbjct: 93  EELEEVAAAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLESNS 152

Query: 123 PATALLRYASESGIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKR--RV 182
            A A+ +  ++  I  L++G   +   +R      + +SI  +      +YV+      +
Sbjct: 153 VAVAITKEVNQHLISNLIIGRSSQAASSRNY---DITASISASVSNLCTVYVVSNGGVHI 212

Query: 183 ITRTTSATTAPSTGQTNSRQWMLGDTDYYRGSSGISEQS---SGTFSSSSRSNVHR---- 242
           + + TS T    T           ++ + R SS  S  S   S   S++ +SN H     
Sbjct: 213 LAKDTSDTERNDTSI---------ESGFERTSSSCSSGSGANSDVMSNALKSNPHTLSNK 272

Query: 243 ---------RGDS--LEANSTEQ---LNSLSTLTEEEDMRAEVESLQ------------- 302
                    RG S  +E +STE        S   EE   R+  E+ +             
Sbjct: 273 RMQNLPTIVRGVSVPMETSSTESDETKKRSSDAAEEASKRSSPETSRSVSWNPQFRDFDE 332

Query: 303 -------------------------LELETTVSLYKQTCEELVHAQKKVQSLTSECLEES 362
                                     + + T++   +   EL HA +       E L+ S
Sbjct: 333 RKDAMSSMSSNFEYGNVVTPLGHYFTDNQDTLNEISKLRAELRHAHEMYAVAQVETLDAS 392

Query: 363 RRVNAALEREQALRKVAAEEKAKHLEAMKELE--AAKDLLAKKAYERQLAELNALKESLE 422
           R++N     E  L     E + K + A KE E    K    ++A +R+ AE+ A  E+ E
Sbjct: 393 RKLNELKFEELTL----LEHETKGI-AKKETEKFEQKRREEREAAQRREAEMKATHEAKE 452

Query: 423 KQKIVDTLLTNDR-RYRRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVL 482
           K+K+ ++ L   + +Y+ +T  EI  AT+ F     IG G YG VYKC+L HT  AVKVL
Sbjct: 453 KEKLEESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVL 512

Query: 483 QHDVLEKKDEFLKEVEILSQIRHPHVVLLLGACPER------------------------ 542
                    +F +E+EILS+IRHPH+VLLLGACP+                         
Sbjct: 513 HSAESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQ 572

Query: 543 --------------------------------------------------DVGMAKII-- 602
                                                             DVG++ +I  
Sbjct: 573 PIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQA 632

Query: 603 GDIVPDNVTEYRNTIIAGTLHYLDPEYQRTGTLRPKSDT--------------------- 604
            D +    T Y+ T   GTL Y+DPEYQRTG + PKSD                      
Sbjct: 633 ADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALTY 692

BLAST of Sgr022292 vs. ExPASy TrEMBL
Match: A0A6J1DK63 (E3 ubiquitin ligase OS=Momordica charantia OX=3673 GN=LOC111021851 PE=4 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 1.6e-266
Identity = 531/736 (72.15%), Postives = 558/736 (75.82%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVAVNG RG  GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV
Sbjct: 1   MTSVAVAVNGGRG--GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 120
           SELDADVVALYVHDVKEKYEQVF PFKKLC REKFLLLVETLVLEDDNPATALLRY SES
Sbjct: 61  SELDADVVALYVHDVKEKYEQVFAPFKKLCRREKFLLLVETLVLEDDNPATALLRYVSES 120

Query: 121 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 180
           GIKRLVLGSCFR+CI RKL GPSVPS+ILRAAPASFDIYVIYK RVITR T+  TAPST 
Sbjct: 121 GIKRLVLGSCFRSCIARKLKGPSVPSAILRAAPASFDIYVIYKGRVITRPTA--TAPST- 180

Query: 181 QTNSRQWMLGDTDYYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQLNSLSTLTE 240
           +T+SRQWMLGDTDYYRGS  ISEQSS TFSSSS SNVH+RGDSLE NSTEQLNSLSTLTE
Sbjct: 181 ETDSRQWMLGDTDYYRGSGAISEQSSATFSSSSVSNVHQRGDSLEGNSTEQLNSLSTLTE 240

Query: 241 EEDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQAL 300
           EEDMRAEVESLQLELETTVSLYK+ CEELVHAQK+VQSLTSECLEESRRV+ ALEREQAL
Sbjct: 241 EEDMRAEVESLQLELETTVSLYKRACEELVHAQKQVQSLTSECLEESRRVSEALEREQAL 300

Query: 301 RKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRY 360
           R VA+EEKAKHLEAMKELE AKDLLAK+AYERQLA+LNALKES+EKQKIVDTLLTNDRRY
Sbjct: 301 RTVASEEKAKHLEAMKELEEAKDLLAKEAYERQLADLNALKESVEKQKIVDTLLTNDRRY 360

Query: 361 RRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVE 420
           RRYT AEIEAATNFFE VNVIGEGGYGKVY+CSLDHTPVA+KVLQHDVLEKKDEFLKEVE
Sbjct: 361 RRYTPAEIEAATNFFEQVNVIGEGGYGKVYRCSLDHTPVAIKVLQHDVLEKKDEFLKEVE 420

Query: 421 ILSQIRHPHVVLLLGACPER---------------------------------------- 480
           ILSQIRHPHVVLLLGACPE+                                        
Sbjct: 421 ILSQIRHPHVVLLLGACPEQGCLIYEYMENGSLDDHILLRNGKTPLPWFVRFRIAFQVAS 480

Query: 481 ----------------------------------DVGMAKIIGDIVPDNVTEYRNTIIAG 540
                                             DVGMAKIIGDIVPDNVTEYRNTI+AG
Sbjct: 481 GLAFLHNSNPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAG 540

Query: 541 TLHYLDPEYQRTGTLRPKSDT--------------------------------------S 600
           TLHY+DPEYQRTGTLRPKSDT                                      S
Sbjct: 541 TLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVESSIASSSLGDILDRS 600

Query: 601 ITDWPLAEAEELARLALKCLKLRCRDRPDLETEVLPVLKSLVDFADACQMKNK------- 609
           ITDWPLAEAE LARLALKCLKLRCRDRPDL+TEVLPVLK LVDFADACQ + K       
Sbjct: 601 ITDWPLAEAEVLARLALKCLKLRCRDRPDLDTEVLPVLKRLVDFADACQNEEKCPANPPS 660

BLAST of Sgr022292 vs. ExPASy TrEMBL
Match: A0A6J1KJP8 (E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111493807 PE=4 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 7.5e-261
Identity = 505/697 (72.45%), Postives = 539/697 (77.33%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGG+GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+F+PFKK+C REKFLLLVETL+LEDDNPATAL+RYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALMRYASES 120

Query: 121 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 180
           GIK LVLGSCFR CI R+L G SVPS+I+RAA   FDIYVIY+RRVITRT  ATTAPS+ 
Sbjct: 121 GIKHLVLGSCFRTCIARRLKGDSVPSAIMRAASRCFDIYVIYRRRVITRT--ATTAPSS- 180

Query: 181 QTNSRQWMLGDTDYYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQLNSLSTLTE 240
           +T+SRQWMLGDTDYYRGSS +SE+S GT SSSS SNVHRRGDSLE NSTEQLNSLSTLTE
Sbjct: 181 ETDSRQWMLGDTDYYRGSSTVSEKSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTE 240

Query: 241 EEDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQAL 300
           EEDM+AEVESLQLELE+TVSLYKQ CEELVHAQKKVQSLTSECLEESR+VNAALEREQAL
Sbjct: 241 EEDMQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQAL 300

Query: 301 RKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRY 360
           RKVAAEEKAKHLEA+KELE AKD LAK+AYERQLAELNALKESLEK+KIVDTLLTNDRRY
Sbjct: 301 RKVAAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRY 360

Query: 361 RRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVE 420
           RRYTTAEIEAATNFF+ VNVIGEGGYGKVY CSLDHTPVA+KV +HD+ E KDEFLKEVE
Sbjct: 361 RRYTTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVE 420

Query: 421 ILSQIRHPHVVLLLGACPER---------------------------------------- 480
           ILSQIRHPHVVLLLGACPER                                        
Sbjct: 421 ILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRLRIVFQVAS 480

Query: 481 ----------------------------------DVGMAKIIGDIVPDNVTEYRNTIIAG 540
                                             DVGMAKIIGDIVPDNVTEYRNTI+AG
Sbjct: 481 GLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAG 540

Query: 541 TLHYLDPEYQRTGTLRPKSDT--------------------------------------S 573
           TLHY+DPEYQRTGTLRPKSDT                                      S
Sbjct: 541 TLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVENSIASGSLADILDKS 600

BLAST of Sgr022292 vs. ExPASy TrEMBL
Match: A0A6J1G4U5 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111450728 PE=4 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 1.8e-259
Identity = 500/696 (71.84%), Postives = 536/696 (77.01%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGG+GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+F+PFKK+C REKFLLLVETL+LEDDNPATAL+RYA+ES
Sbjct: 61  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALMRYATES 120

Query: 121 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 180
           GIK LVLGSCFR CI R+L G SVPS+I+RAA   FD+YVIY+RRVITRT  ATTAPS+ 
Sbjct: 121 GIKHLVLGSCFRTCIARRLKGDSVPSAIMRAASRCFDVYVIYRRRVITRT--ATTAPSS- 180

Query: 181 QTNSRQWMLGDTDYYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQLNSLSTLTE 240
           +T+SRQWMLGDTDYYRGSS +SE+S GT SSSS SNVHRRGDSLE NSTEQLNSLSTLTE
Sbjct: 181 ETDSRQWMLGDTDYYRGSSTVSEKSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTE 240

Query: 241 EEDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQAL 300
           EEDM+AEVESLQLELE+TVSLYKQ CEELVHAQKKVQSLTSECLEESR+VNAALEREQAL
Sbjct: 241 EEDMQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQAL 300

Query: 301 RKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRY 360
           RKVAAEEKAKHLEA+KELE AKD LAK+AYERQLAELNALKESLEK+KIVDTLLTNDRRY
Sbjct: 301 RKVAAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRY 360

Query: 361 RRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVE 420
           RRYTTAEIEAATNFF+ VNVIGEGGYGKVY CSLDHTPVA+KV +HD+ E KDEFLKEVE
Sbjct: 361 RRYTTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVE 420

Query: 421 ILSQIRHPHVVLLLGACPER---------------------------------------- 480
           ILSQIRHPHVVLLLGACPER                                        
Sbjct: 421 ILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRFRIVFQVAS 480

Query: 481 ----------------------------------DVGMAKIIGDIVPDNVTEYRNTIIAG 540
                                             DVGMAKIIGDIVPDNVTEYRNTI+AG
Sbjct: 481 GLALLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAG 540

Query: 541 TLHYLDPEYQRTGTLRPKSDT--------------------------------------S 573
           TLHY+DPEYQRTGTLRP SDT                                      S
Sbjct: 541 TLHYMDPEYQRTGTLRPNSDTYALGVTILQLLTGRQPHGLLLAVENSIASASLADILDKS 600

BLAST of Sgr022292 vs. ExPASy TrEMBL
Match: A0A1S3C9U0 (E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103498614 PE=4 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 8.8e-254
Identity = 501/741 (67.61%), Postives = 546/741 (73.68%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVAVNGVRGG+GGGGSRRAVRWAVENLLPTADRF+LVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFVLVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQVF+PFKKLC REKFLLLVETL+LEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120

Query: 121 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 180
           GIK LVLGSCFR CI RKL G SVPS+I+R A +SFDIYV YKRRVITR   A+TAPST 
Sbjct: 121 GIKCLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITR--KASTAPST- 180

Query: 181 QTNSRQWMLGDTDYYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQLNSLSTLTE 240
           +T+SRQWMLGDTDYY GSS +S +S GT S SS S  H+RGDS+  +STEQLNSL TLTE
Sbjct: 181 ETDSRQWMLGDTDYYMGSSAVSAKSLGTLSLSSLSISHQRGDSIGVDSTEQLNSLHTLTE 240

Query: 241 EEDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQAL 300
           EEDM+AEVESLQLELETTVSLYKQ CEELVH QKKVQSLTSE LEESR+V AALERE++L
Sbjct: 241 EEDMQAEVESLQLELETTVSLYKQACEELVHTQKKVQSLTSEYLEESRKVTAALEREESL 300

Query: 301 RKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRY 360
           RK AA+EKAKHLEA+KELE AKDLLAK+AYERQLAELNA+KES+EK+KIVDTLLTNDRRY
Sbjct: 301 RKAAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELNAIKESVEKEKIVDTLLTNDRRY 360

Query: 361 RRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVE 420
           RRYTTAEIEAATNFF  VNVIGEGGYGKVYKCSLDHTPVA+KV+QHD+ EKKDEFLKEVE
Sbjct: 361 RRYTTAEIEAATNFFNEVNVIGEGGYGKVYKCSLDHTPVAIKVIQHDIYEKKDEFLKEVE 420

Query: 421 ILSQIRHPHVVLLLGACPER---------------------------------------- 480
           ILSQIRHPHVVLLLGACPER                                        
Sbjct: 421 ILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHIFLRNGKAPLPWSTRFRIVFQVAS 480

Query: 481 ----------------------------------DVGMAKIIGDIVPDNVTEYRNTIIAG 540
                                             DVGMAKIIGDIVPDNVTEYRNT++AG
Sbjct: 481 GLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTVLAG 540

Query: 541 TLHYLDPEYQRTGTLRPKSDT--------------------------------------S 600
           TLHY+DPEYQRTGTLRPKSDT                                      S
Sbjct: 541 TLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKS 600

Query: 601 ITDWPLAEAEELARLALKCLKLRCRDRPDLETEVLPVLKSLVDFADACQMKNK------- 615
           I+DWPLA+AEELARLALKCLKLRCRDRPDLE+EVLPVLK LVDFAD  Q ++K       
Sbjct: 601 ISDWPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDFADTFQKEDKGFGNPPS 660

BLAST of Sgr022292 vs. ExPASy TrEMBL
Match: A0A5D3CHP5 (E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001070 PE=4 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 9.1e-251
Identity = 494/717 (68.90%), Postives = 531/717 (74.06%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVAVNGVRGG+GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQVF+PFKKLC REKFLLLVETL+LEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120

Query: 121 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 180
           GIK LVLGSCFR CI RKL G SVPS+I+R A +SFDIYV YKRRVITR   A+TAPST 
Sbjct: 121 GIKCLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITR--KASTAPST- 180

Query: 181 QTNSRQWMLGDTDYYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQLNSLSTLTE 240
           +T+SRQWMLGDTDYY GSS +S +S GT S SS S  H+RGDS+  +STEQLNSL TLTE
Sbjct: 181 ETDSRQWMLGDTDYYMGSSAVSAKSLGTLSLSSLSISHQRGDSIGVDSTEQLNSLHTLTE 240

Query: 241 E------------------EDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSE 300
           E                  EDM+AEVESLQLELETTVSLYKQ CEELVH QKKVQSLTSE
Sbjct: 241 EVFVFLFYCFIVSSFASISEDMQAEVESLQLELETTVSLYKQACEELVHTQKKVQSLTSE 300

Query: 301 CLEESRRVNAALEREQALRKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKE 360
            LEESR+V AALERE++LRK AA+EKAKHLEA+KELE AKDLLAK+AYERQLAELNA+KE
Sbjct: 301 YLEESRKVTAALEREESLRKAAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELNAIKE 360

Query: 361 SLEKQKIVDTLLTNDRRYRRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVK 420
           S+EK+KIVDTLLTNDRRYRRYTTAEIEAATNFF  VNVIGEGGYGKVYKCSLDHTPVA+K
Sbjct: 361 SVEKEKIVDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKCSLDHTPVAIK 420

Query: 421 VLQHDVLEKKDEFLKEVEILSQIRHPHVVLLLGACPER---------------------- 480
           V+QHD+ EKKDEFLKEVEILSQIRHPHVVLLLGACPER                      
Sbjct: 421 VIQHDIYEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHIFLRNG 480

Query: 481 ----------------------------------------------------DVGMAKII 540
                                                               DVGMAKII
Sbjct: 481 KAPLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKII 540

Query: 541 GDIVPDNVTEYRNTIIAGTLHYLDPEYQRTGTLRPKSDT--------------------- 573
           GDIVPDNVTEYRNT++AGTLHY+DPEYQRTGTLRPKSDT                     
Sbjct: 541 GDIVPDNVTEYRNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLL 600

BLAST of Sgr022292 vs. TAIR 10
Match: AT5G57035.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 520.8 bits (1340), Expect = 3.2e-147
Identity = 317/737 (43.01%), Postives = 433/737 (58.75%), Query Frame = 0

Query: 2   TSVAVAVNGVRGGR-GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 61
           +SV+VAV G  G   GG  SRRA+RW +EN LP  DR +LVHVMP +T+IP+P G ++ +
Sbjct: 19  SSVSVAVKGSVGDAVGGTASRRALRWTIENFLPKIDRLVLVHVMPTVTTIPSPSGSKIPI 78

Query: 62  SELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASES 121
            ELD  VV++Y  D+++++EQVF+PFK++C   K    VETL+LE  +PA ALL+Y S++
Sbjct: 79  EELDESVVSMYKRDLRKEFEQVFVPFKRICKSNK----VETLLLEHHDPAKALLKYMSDT 138

Query: 122 GIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG 181
            ++ LV+GSC  N +TRK  G  +P ++L  AP + +IYV+ K R++T++T+  TA S+ 
Sbjct: 139 DVECLVIGSCSSNFLTRKK-GQEMPLTVLGEAPETCEIYVVCKDRILTKSTNQFTADSSS 198

Query: 182 QTNSRQWMLGDTD-YYRGSSGISEQSSGTFSSSSRSNVHRRGDSLEANSTEQL------- 241
                +     T+ + R  S  +  S+ + +SS R  + R G    ++ T ++       
Sbjct: 199 SFRIPEGAEAYTESFSRTRSDKTGLSASSITSSGRMRIGRPGSLPHSHPTSRVYSDAQSS 258

Query: 242 ------------------NSLST-----------LTEEEDMRAEVESLQLELETTVSLYK 301
                             +++ST            T +  +RAEVE L+ E++TT+S+YK
Sbjct: 259 STDIVLVDDEHCRSILRHSTVSTSKIQMDPRPHLKTPKSGVRAEVEQLRKEVQTTLSMYK 318

Query: 302 QTCEELVHAQKKVQSLTSECLEESRRVNAALEREQALRKVAAEEKAKHLEAMKELEAAKD 361
           Q CEELVH Q +VQSL+SEC++E+ RV  ALE+E+  RK AAEEK KHL+A+KE+E AK 
Sbjct: 319 QACEELVHKQTQVQSLSSECIKETERVITALEKEEMRRKAAAEEKEKHLKAVKEVEEAKS 378

Query: 362 LLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRYTTAEIEAATNFFEPVNVIGE 421
           +LAK+  ERQLAEL+ALK+S+EKQK+++ L   D RYR+YT  EI AAT+ F    +IGE
Sbjct: 379 MLAKEFCERQLAELDALKQSIEKQKVIEQLFLRDGRYRKYTKEEIAAATDNFSSRKIIGE 438

Query: 422 GGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVEILSQIRHPHVVLLLGACPER--- 481
           GGYGKVYKCSLDHTPVA+KVL+ D +EKK+EFLKE+ +LSQ+RHPHVVLLLGACPE    
Sbjct: 439 GGYGKVYKCSLDHTPVALKVLKPDSVEKKEEFLKEISVLSQLRHPHVVLLLGACPENGCL 498

Query: 482 ------------------------------------------------------------ 541
                                                                       
Sbjct: 499 VYEYMENGSLDCHISPKKGKPSLSWFIRFRIIYETACGLAFLHNSKPEPIVHRDLKPGNI 558

Query: 542 -----------DVGMAKIIGDIVPDNVTEYRNTIIAGTLHYLDPEYQRTGTLRPKSDT-- 573
                      DVG+AK++ D  PD+VT YRN+IIAGTL+Y+DPEYQRTGT+RPKSD   
Sbjct: 559 LLDRNFVSKIGDVGLAKLMSDEAPDSVTVYRNSIIAGTLYYMDPEYQRTGTIRPKSDLYA 618

BLAST of Sgr022292 vs. TAIR 10
Match: AT2G19410.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 434.9 bits (1117), Expect = 2.3e-121
Identity = 299/781 (38.28%), Postives = 393/781 (50.32%), Query Frame = 0

Query: 4   VAVAVNGVRGGR-GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTP--------- 63
           VAVAV G+ G + GG GSRRAVRWAV+NLLP AD+F+++HV+P ITSIPTP         
Sbjct: 24  VAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKADKFVMIHVIPTITSIPTPNILILMFTR 83

Query: 64  -----MGDRVAVSELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDN 123
                 GDR+ V E++  VV +YV DVK++YE VF+PF K+C                  
Sbjct: 84  MWVVTAGDRLPVEEVEESVVEMYVRDVKKEYETVFVPFLKMCK----------------- 143

Query: 124 PATALLRYASESGIKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVIT 183
                    S    KR      FR   +R+  G  VP ++LR AP + ++Y++ K R+ T
Sbjct: 144 ---------STRSTKRY-----FR---SRRTKGTGVPLTVLRYAPETCEVYIVCKDRITT 203

Query: 184 RT---------TSATTAPSTGQTNSRQWMLGDTDYYRGSSGISEQS--SGTFSSSSRSNV 243
           ++          ++  A +T     R W          +     QS  +GT  S+S   +
Sbjct: 204 KSMDPLINREPCTSPHAAATAHDFLRDWAASFHTLRSPTLPDPRQSTEAGTRRSASAREL 263

Query: 244 HRRGDSLEAN--STEQLNSLSTLTEEE--------------------------------- 303
                SL  N   T Q +  S+ T  E                                 
Sbjct: 264 RFEALSLTCNKPKTPQSSKASSATTPEIFRRRRGSDIPQLNYSDFDKTCTKPQSNVENIV 323

Query: 304 --------------------DMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQSLTSE 363
                               ++  EVE L+ EL++TV  YKQ CEEL   Q KV+ L++E
Sbjct: 324 SEHRDSDRSPPETSRKSKKVEIEEEVERLKNELQSTVFKYKQACEELFSTQNKVKMLSTE 383

Query: 364 CLEESRRVNAALEREQALRKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELNALKE 423
            L ES+RVN A+E+E+  R  AA EK ++++A+KE+E AK LLA++  +RQ+AE+NAL+ 
Sbjct: 384 YLNESKRVNNAVEKEELQRNTAALEKERYMKAVKEVETAKALLAREFCQRQIAEVNALRT 443

Query: 424 SLEKQKIVDTLLTNDRRYRRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTPVAVK 483
            LEK+K++D LL  D RYR+YT  EI  AT  F P  VIGEGGYGKVY+CSLD TP AVK
Sbjct: 444 YLEKKKVIDQLLGTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLDSTPAAVK 503

Query: 484 VLQHDVLEKKDEFLKEVEILSQIRHPHVVLLLGACPER---------------------- 543
           V++ D  EKK EFLKEVE+LSQ+RHPHVVLLLGACPE                       
Sbjct: 504 VVRLDTPEKKQEFLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGSLEEYIFHRKN 563

Query: 544 ----------------------------------------------------DVGMAKII 577
                                                               DVG+AK++
Sbjct: 564 KPPLPWFIRFRVIFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSKIADVGLAKLV 623

BLAST of Sgr022292 vs. TAIR 10
Match: AT5G61550.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 247.7 bits (631), Expect = 5.2e-65
Identity = 227/805 (28.20%), Postives = 341/805 (42.36%), Query Frame = 0

Query: 3   SVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTA-DRFILVHVMPKITSIPTPMGDRVAVS 62
           SVAVA+NG +       S+  V WA+E  +P     F L++V P ++ IPTPMG  VAVS
Sbjct: 23  SVAVAINGKK------KSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVS 82

Query: 63  ELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASESG 122
           EL  DVV+ Y  ++     ++  P+KK+  R K  + VE L+L+   PA A+    + +G
Sbjct: 83  ELREDVVSAYKQELDWSANEMLRPYKKMFERRK--VQVEVLLLDSLEPAAAIAEEIAGTG 142

Query: 123 IKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTGQ 182
           + +LV+G   R   +RK+    + S I  A P    +YVI K ++ +   S + A  + +
Sbjct: 143 VTKLVIGMSLRGFFSRKI---DMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIR 202

Query: 183 TNSRQWMLGDTDYYR------------------------------GSSGISE---QSSGT 242
                   G TD  R                              GS+ +++    SSGT
Sbjct: 203 FERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGT 262

Query: 243 --------------------------------------------------------FSSS 302
                                                                     SS
Sbjct: 263 DQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSS 322

Query: 303 SRSNVHRRGD---------------SLEANSTEQLNSLSTLTEEE-DMRAEVESLQLELE 362
           S SN H   +                  ++ ++ L S+ ++T+ + ++  E+E L+ EL+
Sbjct: 323 SSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELK 382

Query: 363 TTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQALRKVAAEEKAKHLEAMK 422
               +Y     E V A KK+  L     EES ++    E+E+  +  A++EK ++ EAMK
Sbjct: 383 HVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMK 442

Query: 423 ELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRYTTAEIEAATNFFE 482
           E E  K+L+ K+A  R+ AE  A +++ EK K+  +L++   +Y+ YT  EI AAT+ F 
Sbjct: 443 EAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFA 502

Query: 483 PVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVEILSQIRHPHVVLLLGA 542
               IG G YG VYKC+L HT  AVKVL     +   +F +E+EILS+IRHPH+VLLLGA
Sbjct: 503 ENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGA 562

Query: 543 CPER-------------------------------------------------------- 573
           CPER                                                        
Sbjct: 563 CPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHR 622

BLAST of Sgr022292 vs. TAIR 10
Match: AT5G61550.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 247.7 bits (631), Expect = 5.2e-65
Identity = 227/805 (28.20%), Postives = 341/805 (42.36%), Query Frame = 0

Query: 3   SVAVAVNGVRGGRGGGGSRRAVRWAVENLLPTA-DRFILVHVMPKITSIPTPMGDRVAVS 62
           SVAVA+NG +       S+  V WA+E  +P     F L++V P ++ IPTPMG  VAVS
Sbjct: 23  SVAVAINGKK------KSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVS 82

Query: 63  ELDADVVALYVHDVKEKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASESG 122
           EL  DVV+ Y  ++     ++  P+KK+  R K  + VE L+L+   PA A+    + +G
Sbjct: 83  ELREDVVSAYKQELDWSANEMLRPYKKMFERRK--VQVEVLLLDSLEPAAAIAEEIAGTG 142

Query: 123 IKRLVLGSCFRNCITRKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTGQ 182
           + +LV+G   R   +RK+    + S I  A P    +YVI K ++ +   S + A  + +
Sbjct: 143 VTKLVIGMSLRGFFSRKI---DMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIR 202

Query: 183 TNSRQWMLGDTDYYR------------------------------GSSGISE---QSSGT 242
                   G TD  R                              GS+ +++    SSGT
Sbjct: 203 FERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGT 262

Query: 243 --------------------------------------------------------FSSS 302
                                                                     SS
Sbjct: 263 DQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSS 322

Query: 303 SRSNVHRRGD---------------SLEANSTEQLNSLSTLTEEE-DMRAEVESLQLELE 362
           S SN H   +                  ++ ++ L S+ ++T+ + ++  E+E L+ EL+
Sbjct: 323 SSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELK 382

Query: 363 TTVSLYKQTCEELVHAQKKVQSLTSECLEESRRVNAALEREQALRKVAAEEKAKHLEAMK 422
               +Y     E V A KK+  L     EES ++    E+E+  +  A++EK ++ EAMK
Sbjct: 383 HVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMK 442

Query: 423 ELEAAKDLLAKKAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRYTTAEIEAATNFFE 482
           E E  K+L+ K+A  R+ AE  A +++ EK K+  +L++   +Y+ YT  EI AAT+ F 
Sbjct: 443 EAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFA 502

Query: 483 PVNVIGEGGYGKVYKCSLDHTPVAVKVLQHDVLEKKDEFLKEVEILSQIRHPHVVLLLGA 542
               IG G YG VYKC+L HT  AVKVL     +   +F +E+EILS+IRHPH+VLLLGA
Sbjct: 503 ENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGA 562

Query: 543 CPER-------------------------------------------------------- 573
           CPER                                                        
Sbjct: 563 CPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHR 622

BLAST of Sgr022292 vs. TAIR 10
Match: AT2G24370.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 246.5 bits (628), Expect = 1.2e-64
Identity = 212/728 (29.12%), Postives = 321/728 (44.09%), Query Frame = 0

Query: 20  SRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAVSELDADV---VALYVHDVK 79
           S+ A++WAV+NLL      ILVHV  +    P+P+ +  ++    A +    +L   D +
Sbjct: 30  SQHALKWAVDNLLQRGQSVILVHVKLR----PSPLNNSASLHASSAKLSQDSSLVCRDPE 89

Query: 80  EKYEQVFIPFKKLCSREKFLLLVETLVLEDDNPATALLRYASESGIKRLVLGSCFRNCIT 139
              +++F+PF+  C+R+   +  + ++LE+ + A AL+ Y +++ I+ LV+GS  +    
Sbjct: 90  GISKEIFLPFRCFCTRKD--IQCQDVLLEESDVAKALVEYVNQAAIEVLVVGSSSKGGFL 149

Query: 140 RKLIGPSVPSSILRAAPASFDIYVIYKRRVITRTTSATTAPSTG---------------- 199
           R      +P SI + AP    +Y+I K ++ T  +++ +AP T                 
Sbjct: 150 RFNKPTDIPGSITKNAPDFCTVYIISKGKIQTMRSASRSAPMTAPLRSSVQPPSLKPPQP 209

Query: 200 ----------------QTNSRQWM---------------LGD------TDYYRGSSGIS- 259
                           ++N R+ +               + D      + + R  +G S 
Sbjct: 210 MPSTSANSLRADRRSFESNQRRSVEDQQRRSMEDQQRRSIDDQSESFRSPFTRRGNGRSY 269

Query: 260 -----EQSSGTFSSSSRSNVHRRGDSLEANS----------------------------- 319
                 +S  +F SS R ++ R   SL  N+                             
Sbjct: 270 GDLTVPESDISFISSGRPSIDRIFPSLYDNNDPSRTPPRLSNFSDMDYSSSLDQSSNYGR 329

Query: 320 -TEQLNSLSTLTEE---------EDMRAEVESLQLELETTVSLYKQTCEELVHAQKKVQS 379
            +  +NS +    E         +D+ AE+  L+LEL+ T+ +Y   C+E + A++K   
Sbjct: 330 RSVDMNSPTDFESERFSSASQSIDDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKATE 389

Query: 380 LTSECLEESRRVNAALEREQALRKVAAEEKAKHLEAMKELEAAKDLLAKKAYERQLAELN 439
           L    LEE R++  A   E+A   +A +EKAK   AM+  EAA+ +   +A +R  AE+ 
Sbjct: 390 LQRWKLEEERKLEEARNAEEAALAIAEKEKAKSKAAMEAAEAAQRIAELEAKKRVNAEMK 449

Query: 440 ALKESLEKQKIVDTLLTNDRRYRRYTTAEIEAATNFFEPVNVIGEGGYGKVYKCSLDHTP 499
           ALKES EK K +  L  +D RYR+Y+  +IE AT FF     IGEGGYG VYKC LDHTP
Sbjct: 450 ALKESEEKTKALTALANSDVRYRKYSIEDIELATEFFAEKYKIGEGGYGPVYKCYLDHTP 509

Query: 500 VAVKVLQHDVLEKKDEFLKEVEILSQIRHPHVVLLLGACPE------------------- 535
           VAVKVL+ D  + + +F +EVE+LS IRHP++VLLLGACPE                   
Sbjct: 510 VAVKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPECGCLVYEFMANGSLEDRLF 569