Sgr021835 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr021835
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionsn1-specific diacylglycerol lipase beta isoform X1
Locationtig00153840: 450578 .. 500572 (-)
RNA-Seq ExpressionSgr021835
SyntenySgr021835
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGCGAAAAGTGGAATCATGAAGAGATAGTTGGACAAATGGCGCAAAGAAGGGACCAAGAAACTTCTACCTTCCATGAACCATTGATTCCCTCCGTTTGCTCTTCAAACCACTCGAACCATCAAATTCGATCACCCATCCCCACATTTCGATCACCATGGTGTCCCATTCGAAGTAGAACGGAGGATATGGGGGTGTCGAAGTTGGAGAATATTCGGTGCTCAACCATCGTGTTGGCCATTTCTAATGCCCTCGTCGCCCTTCTCGGCGCCTTTCTTCTCATCGTTGTTTATCCTGCTTGTGAACGCCAATATGTCCTCCCATTTTTGGCCGTCTCTATGGCTTCTTGCATTAAAATCGTCACCATGGTTCAGTGTGGCATTGCCCAGGAGGCTACAGCCAGAACCATTCTCGAATCTCCTGGTGATACTGCTGCTGTTGTCAACACCATTATGCGCCACGAGAGACGGGTATTTGGCCAGTTTTAGCGCTTATTATTATTATTATTATTATTATTGAATTTGACAAAAGAAGTTTTGGGGGCAATTTTTGTTTTTAGAGACGAAGCATTAACAGAACCTTTTTATTACGAGAGAATTATCTCAATTATTAAAATGAATCCGGCGTCTATTTACCCTTTTATCTGTCTCAATAGTTGTTTTGGTTAGGTTATAATTATCGAATATTCAAAATTGTTCTATTCTTGCCAACATGAGCATAGCTTAGCGGTTAACACATCCATACTTCTTCTAAGAGGTCGTAGGTTTGAATCCCCCATCCCCAATCTGTGATATTCTCAAAATTGCTCCATCTTCGCTTCATAATTTGATGTCCGGAAACGGAATTGTTGATAAACTTGGGGATTGTGATGGAACATTTCATGTTTGCTGTTGGAGGACACGTATAATGTTGTATTCTATTGCTTGCATTGTTGTCATAGATGACAAAGACTTGTTCACACAGTTAGCATAAATTCAATAAACTTGCTGTTCTAAATCCAAGACGTGCCTTGCAGGAGTTAGGTTAAAAACGAATATTAAGATGTTTTTTTTTTAAATAGGAAACAAAAGTGAGAGTAAAAAAAATTTACTAAAAAGAACCCAACTTTTGGTAATAAAAAATAAAGGATAATTACGAAACAAATATGAATTATGGGCCCAAGAAGCATTAAAATGAACAAATCCCACACATCTTTTCACAATCTATGTAACCCCTAAATGTTTGTCTATTTCTTAATCATACAAAATTAAATGTTTTTTAGATGTTGATGGGTCAAGACTTAAGGAAGAGAGAAAATCAACTTTGGGATATTGTGATTTTCTTCGTGGTAATTTAGTTTTGTGGAAGAGTAAGAAACATAATATGGTATCATGCTCAAATGTTGAATTGGAATATAGAGTGATGACTTAGTTTGTGTGTGAAGTGATGTAGATATATCAATTAATGCATGAGTTTATTATCACAACTCCAAACCTATCGTGGTGACAATTAAGCAGCACTCTATATCACTTCTAATATAGTATTTCATCAACAAATTATATATAGTGAAGTTGACTATCATTTTGTTAAGGAGAAACTACAATGAATTTTAGTGTTCACAGAATATTTGAAGATTGGGAGTAATTGGAACATTGTTTCACGATGACATTTAATGGAGAGCTTGTAGACTTTCTTTCTTACAAGTTGGACATGACTAACATAGATGCTCTAGCTTGAGGGGGAATGTTATGTATATTAGTATATAGTGTTGCAAGTAGCTTAGTTATAAGTCCATTGGTGGGATAGCCAACTTTCTTCCTTTCTTTCTCTCTCTTTCTCGCTGATGAGAGTCAAGGAGGGACTTTCCCTCACCGTGGAGATTGAAAGAAAGAGATTTTAATCTAGCAAAAGAGATTTTAGTCCCTCTATTTTGTAATAAAGGCCATGTCATGTGTTGGTTTTTTTGTTGTTTTGTGGAGCATTTGGCTTGAGAGGAATAAAAGGATTTTTAGATGGGTTGAGAGGTCTTGGGAGGAAGTTTGGTCTCTTTTTAGGTTTAAAGCCTCTCTTTGGGCATTGGTTTCAACGAAGTTTTGTCGTTATCCTTTAGGTATTATTATTTTGGATTGAAGCCCCTTTTTTGTATAGTTAGTTCAGGTTTTGGTTTGGCTCCTTTTGTTGGGTTGAGGTTGCCATTTTGTATGGCTTTTATACCTTTCATTTTTCTTGATGAAAGTTCGGTTTTTCGTTAAAAAAATGGTCCATTAGTAATATTGTCCTTTATTATAATTTCAGCATTTTTTATTTTAGGGCACCTTGTACACCTGCATACATGGCTTTGATGAATGAAGAAGATCATAGTGTTTTCTCCCAAACCTCTATAGAGCTATAGTAAGGGTAATCAGGCGATTTTAGATAGAAAGTTTTTGTCAAGTAGGATTTATGTTATGGGTTGTAACAATAACTTTTCACTAAGTTAATAAAAAGTTGTTTTTGTTTTTTTTTTTACAAAAAAACATCTAGTTCTAGAAGCCTCCTAAACATTTCTATAAATCATGGTGAAAGGTTGAGAAGCTTTGTGAATTTGAGGAGGATAAGAATTACGAATACACTTAGACATTTCACAAATTTTACATCGAAATAAATGAGGATAATTATTGAGAACTCTTTTGAGATAATTTTTAGGGTGACCCACGTGATATGCTATAGCATAACTTTTACCACTTTCAAGGATAATGACCATGGCTTTATTACTGAGCTGTGCTAAGGGTTGATTCGAAATGAGGATGTAAAATTTTGATTGAACACCGTCAATTGTATTCCTTGTACTCAAATCCTAAATATTGTACATTTGATTTTGAGTGTGTTTACTAAAAGAAAGTAAGTTACATATAATTTTTGGAACAAATAAACTTATGAATAAGTTGTATAGAAAGAGCAATTGATCCTACTCTTGGAACTCTAACTAGAGTTCTATAAGCAATGTGCACTTTAAGAGCATTATAACAAGGTTCATAGTTTCTTAAAATAGAGATATTACCTATCATGTGGTCAGAGGCCCTTGAGTATACAATCCATGAGCTCAAGGATTTTGTTTTGCCCTTAGATCATTCAAGGAACTACTTGAGTCACAGTTGCGATTCCATTGTTGGGAGTAGGAGCATTTGAATTCAGGTTAACTTGTCCAATAATTTCCGAAAAAATTCTTAACTCTTCCTAGGTGAAGAGTTTGCTTTAAGTAGCATTGTTCTCATTTGAGTAGTTACATTTGCATGATTTTCTTGATTTTACCTTTTACTTCAAGTTATGTTGGGCTTTCAATGATGTGGTTTCCCATGGATTTTCCAACCTATCTTCCTTGCATGTTCAATCTTCTTGCAATGATCACACCGAAGCTTGAGTTTTGTTTGTCGATTTTTAGTAAAGTAGGATAGATTATTTTAAGTAGCCAAAGTTGTGCCTTCATTTGGTAAATGGTTAGTAGTATTATTTGCTCCCAACATCAATTTTTTCTAGCTCTCTTCCCTTCAAACCTCTGCAAACACTTCTCTTATAATATTGGGTAATGATTTTGTTGAGTCTAAGTTAGAATTTGAAGATTGCAACCAAAGTAAGAATTTGAAGATTGCAAACACTTCTGGGAGTTTTTGAGTTAATGTTGGATCTCAAAGTCAATGGGGAAGTGTTATTTGTATGGGATTAATTGTGATGTAGGTAGAAGGTGGGCTAGTCTTTTTCGGTGAGGTTGTTTCTCTTCTCTTGTCTTACATATGCCAACCCTTGGGACACAATTCTAGAGCGAGCTCTTTTTGGGACCGATTGTGGAAAAGGTTCAGAAGAGACCTTTGCCTTGAAGGAAAATTTTTTTCTCCAAAAGGAGAAGATTCGCCGTATTCCAATATGTGCTAAGTGGTATCTCTTTGTATTTCCTTTTTCTCTTCAAAATTCCAAGTTTTATTAAGGATAAGTTCGAAAAGGCTACAAGAGACTTCTTATGGGAAGGGGTAAAGGAGGGGGTGGGTCAAATTTGATGAATTGAAATATTGTGTTAAAACCTTGTGAGGAAGGGGGTTGGTAATAGGAAATTTAGGCAGCAAGAACATAGCCCTTCTCTCTAAATGGTTGTGGAGGTTTCCTTTAAACCTTTCTAACTTTGCGACATCATATAAATGTGAGTACGAGTTTCATTCTAATGATTGGCATGTCGGTTCAATTTCTAAGGGCACCTTCTAAAATCATTGGAAGGTGATTTTCCTATGTCTTTCGAGTTTCCTCCCCTTTTATCTTTTATGTAGGTGATGGTAGTTGAGTTCGGTTCTGGGAGGATCATTGGATCAGTTTAGGTCCTCTCCAATTGTAGTTCCTTTGGCCTCTTCCAAAATCTTCTTATCTCTTTTGTCATTCTTTTGAAAGTCCTCCCTCTTTTGAATTTTGTTTGGGCCTTTTGTGATTGCGAGATTTTCGGGGACCCTTTCTTGCAAACCTTTGTTTAGTCGGTTGGTATCATCTTCAATCCTCTCTCCTTCTTGCTCTTTATTCTCTACTTTGTGGAAGTTAAAAATTCCGAAAAAGATACAATCCTTTGCTTGGCTTGTAGCCTTGGAAAGGATTAATACCATAGACGTTCTCTAGAAAGTTTCCTCTTTTATTCTTGTCCCTAACTGGTGTGTTTTTTTTGCAAGAGTAGGGTAAAGGATTTGAGCTATATTTTGTGGTTATGTCCTTTCATGTCTAGGGTGTGGGGTCGTTTGTGGTAGACTTTTGGTTTATCTTTAGTTTAGAGGTCAAAGCATTCACTAGATGATCGGAAGGTTGTTTTGAATCCTCCTTTATAATGGAAAGACCATTTGTTTGGCAGATTGGTTTCTTCAGCCATTCTGTGGTTCATATGGTTGGAAAGAAACCGAAATATGTTCTTGGGATGGATAGCTTGTGGAAAGATGTGTGGAGGTGGCACATTTTTTACGTTTCTCTTTGGGTTTATATAAAAATTTATTTATTTGTTTAGAATAATACAATAGGGTATAGACTCGAACTTACAACGTTATTAATGCCTAAACCAGTTGAGCTATGCTCAGGTTCTCAGAATTCAAATTTGTTTTGTAATTATCTGTTAATTTTTATTACCAAAAGTTTGGAAATCTTTTGTACAAGTTTTTGTTTACCCCCTTGGTTTGACCCATTTTTCTGTGATGGTAGAGATGAAAGCTGGTTTCTCAAAAAAAAGGTCATAATTTACAGATTTTTTCTTCTAAATATTTTCTTGTACTTAGTGGTCTCCTGTGCATTCCCAATCAGTAAACTTTAGGTCTAGTTGTTGCCAAGGCTAAAATATTGAGTTACAGACATTTCCTCGTCCTAAGTCATGTAGGAGAATTTCAATTTTAAATGACTCACAAGTATTCTCCAAATGAGAAAAAGTTTCTTTTATAGCGTCCCTAGTTTCCAGCGAAGTAGAAAATTCTCCCTATTCTCATTAGTTATGGAATTGATTAGCCAGGACAGTACATGCTGTTGTAGCATTTCAGACTTTATATTTGGGATCAATAACTGCTGGTGCTGTAGTTGTTTTAGTGCGACAATCATCCTTACCTTTTCTATCTATGAACATCATCACATATTATGACCCTCTTAGAGAGTTATTTCCGTTTAATTTATTCTCATAAGTAGGAATTGAAAGACCCATCACCCATTACTGCCAAATTTTGAGTTGGACCATTTCCTGAAATACATTGTCCAATTTTCACGTTGTTGGAAGATTTGGGCATCTTAACCATGGCTGATTCATGTTGCCAACAAACTTGGTGGGTGCGAATGATAGTACTTTGCCCTTAGAGATTGAAGTCACACTCCGATACCATGATGAAAAGAGTCGAGAATATGTCTGATGCTTGTTAATTCAAGTAATTGTACATTTGTACTCTTCTGTACATTATACAACTCTAATACAAATATCTAAGAGTATTTTAAAGAGAAGGAAGCTGGGTAATGTTAATAAGGCAAAGAAGCTATAAAAAGCTAACTAAAAAAGGAAGAACAAAGTACAAATGAGGAGTGAAATGGGTTAAGAGAAAGCCCATGCAGAAGAGGTTTAAATAGAAGAAAAAGGGTCTTTTGAACCTATTAGCTCCTGCCTTCAACAATGTTTATCTAAAAGGAATAAATGCATCATTTATGTTCGCTTGTATAAGGATGTTGACATATTTATTCTCAATATATTGTTTCATGAACAGTTGAGGTATAAAAGATGGCTTTGGTGGACTCGTTTTGCTCTGGTCATTACAGTGCTGCAATTTGTTGGAGCATTTTACTTTATCTTCCATATGGCTAGCTACATTGCTCATGATGACACTTCAAGTGGTTGTGCTTTAGGTAAGGTGGGTGCAATCTCTTATTTATCGTATTGTCTATGTCAAGTCCTACATTTTTACTCTTTTATTCAGTACATGTTGTGGCTGAAAGGTATCTGGTTTGGTATAGGCGCCATGATCTTTTGACTGCATGCTTGCTTTGATCTGTCTATTTTTATGTTCTGCTGTGTTCAATTGGAAATCAATCAATTTTTAGTCACAGGCAGCGTCTCTATCAATGGTTCAGTTTTTACTTGTAAGCAAGGCAGTGTATGCGTTGTTGGTTTAATTACTTTTGGTTTGTATTTGCAGAAACTTATTGTTCTAGTTAAATTTCCTGAGTCTTTATGTTAAGAGCGTGCACTCCAAAAAGCTTTTATTTTCTACATACTACATGTTTGTTGAACCGTCCACAACTAGTATTTTATATTTAGAACACTCCACAATTATTTTATACTGTTAATGGATTGTAAATCCTAGCTGTCCCCCATCACAAAACCATAGTGATAGGAATGCCTTTGGATAGTTAGGAATATTATTAGGATGGTAAAGACATATTAGTAATTAGTTAAGCAGGAGCTATTATAAATAGAGTGAGTGGTAGGAGGAGGAAGGTAGGCAATAGTGTACTTGGTTTAGGCTTGAGTGATCTCAAGAGAGGGAGGGTCCAAGTACCTCGAATTACTTGGGAGTCATTGTAGTTCTTCTATTTTTTATATTTCAATATTACTCTAGTTGTTTGGGTTCTATCAAAGTGGTATCAGAGCAGTTCGATTCTGGCTATGGAGTTAGATCAAGATACAGTGAGTACTTTACGTGAAGAGATTAAACGATTAATGGAACGGATAGACTCAAGGTTCCAATCCATGATTGATAAGATGGATCAGAGAAGAATGGAGTTCCAATCCATGCTCGATAAGACAAGCAAGAAGGAGGCACTGACGAGTATAGAGAAAAAGATTGAGAGGAAGCTGAGCACATTCGAATCCAACAAGAGATTGAGATTAGTAAAAGAATGACAACAAAGACGACTGAGGAAGATCCACCATATGAGGAACAAAGGTAGAAAAAATGGAAGATCAATCGAGAAGCCAATCAAGAAAAAAGTATGAACTGGCAGAGGAAGACAGAGTTCGCACGATCGCGTAAGAACGCATGCAAGAAAGGTAGAGAAGAAATTGAAAATGAGGCTGGCTATCGAAAAACACGATTGAGGGGAGACCGACTAGTAATCCATCGAGAAACACGATTGAGGGGATACCAATTGGTATTCTGTCGAAGTTCGAAAATCATGCGCACGAGGGCTAGGAAGATAATCAATGTGACGAAGGCTGGATTTTCGAAGAGAGATTGATGGTCGTTGCCGATTCTAGGGTAGAAGGTGAACACTCAGAGGAGGAGGCGCCGATGTAAGTTTTGTGGTGGGGCGGCCATGAAGGTGGGTCATGTAAGAAAGAGGGAAAAATGAGGCCTAATCATGTGAGTGGTCATTGCCGCAACAGCAAGTACTATAGTCACCGACCGTCACCCATCACCTTTTGTTGCTTGTACTACAGCCACTGACCATCCCCCTCTGCCACACGTTTGCATGTCATATGCGCCGCACACACCCACACCCGTGTACAACTTCAACGCATTCCTCATCTCACTGCCTCCGTTGTTGTCTCAAACTATTGTTTGCCACTATATCCGACAGTCATCCGCTGCTGTTCATCGCTGCCCGCTGCACACTTGAAGACTCATCCAGTTGGGTTTTTTATTACATAACGTTTGCAAGGGCTAAGCTTCAAGTGTGTCAGACGTGACTAGGTTGGGTTATGTTTGTAAAGAAAAAAGGAGGCAGGGGAGTTAACCACACCTTGGGGACAAAGTGTGTTTGGAGGGCGGGGTAATGATAGGAATGCCTTTGGGTAGTTAGGAATATTATTAGGATGGTAAGGACATATTAGTAATTAGTTAAGTAGGAGCTGTTATAAATAGAGTGAGTGGGAGCAGGAGGAAGGTAGGCAATAGTGTAGTTGGTTTAGGCTTGAGTGATCTCAAGAGAGGGAGGGTCTAAGTACCTCGAATTACTCGGGAGTCATTGTAGTTCTTCTATCTTTTATATTTCAATATTGCTCTAGTTGTTTGGGTTCTATCACATAGCTATTGTGTTTGATAGTTTTTCTTTTGGAATTTGCCTTCAAAAGAAATTTGAATGACAGACCAAGGTCTCATGCTCTCTCCTTCCTTCCTAGTTTGGAATTGTTATGTTTGCATCAGGACATAATAGGAGATGATGATCTTTAAGATCGTGATGTTTTATGTGAAATCTATGTTGAGTTTCTTAGAGCAAGGTAATAGCTTGGTACCATCTATATTGTTTTAATTATGATTTTAAAAAAAATTAATTTCTTGTTTGGTTGAAAATCCTTCATAAAATCTACATTAAAGGAAATTTGGAAGAGATTTTCTGCGAGCTCCTTTAATCCTCAAACATGTTTTATGTGAATTAGTGGGGAATGTTTAGATCGCCTATTTTACAACATGTTTTCTCTAGTAAATCACGTATCATCGATCTTTGACATCTCCTGGTGTGCCCCATTTCTCTGTTTGGGACTTTCTTGTTTCGATTGCTAATTTATAGAAGAAGAAAAGAGGATCTTACGATATGGCTCAACCTTTTCTGTCTTTTGTAAAATTTGTAAAGAGAGATTGGATTTTTAACAATAAAAAAGGGAATATGGGTGAAGTTTAGAACTGTTAGGTTTTTGGTTTGAAATTGGTCGTCTCTTGCCATACTGTTTCTTAGCTATTCGTCCTTTTTTCTTTCTTTCTTGTTCATTTTTCCCTCCACCCTGAATTGTAGCTACTCGAAGAGTACTTCTGTTATCTCCCTAGCTAGTCGGCCGGAATGACTTCTTTGTCTTCGTTCCCTTTTCTGTGCATTTACTTTTTGTACTTGTGCGTGAGTGTTTGAGACTTTCAAATTCTGTTGTACACATTTGATGCAGGGGTGGCGTCAAATGATAGATGGTGGAGACGAAAGCTACTGGTTTTATTTACAATCTTGGTGTGCTTTGTTGCACTGGTTCAATGTTTTACTGGTATGGATGTTCTAAGGTGGAGATCATTTTATGAAACTCAAGATCATGCGTGGAAAGCACATTACAGTGAGATATTTGATCATGGAATTCGTGAGGCATTATGTTGTTTGGGACGTGCTAAATACTTGTATGTCATTTTTATTTAATACGAGGTTTCTCTTATATGCTTTATATTTGTGTTACTCTTTCTAATTTTCCTATCCTGGCATCGTGTTTTAGGAGCGTCATGGAAGAAGATGAGATCTTCTCAGTGGCGCAATTATTGGGTGATCTTGTTGCTTATCGTTCATCTGGCACTGGGCATTTGGAATTTTTGGCAGGTTTAGTTTAAAATTCTGCACAAGTTTTTAATAGTCTTTATGTCATTTTTCATTTGCTATATGTCATTTGTTACATATCTGGATCTTTTAGATAGACACGTATTCTTATTGTATTATGCATTTAGAATTCAGCAACTTTGCTAATGTGAAATTAGTTTATATGAAATCAGTTGGTTTTTGTATCAAAAACTTTTCCTGTCTTGAATTCAAGTCTTAATTGCTTCAGTATTAATTTTAAAATAGTTGGAAGCACTCCTACCAAAAACATATATAAATGGAATCGAAGTGTGCCATTTGAGAAGCGATGGATCTTAAATACAGTAAAGTTCTACAGTGCAGACCCCAAGCAATGTCTGTGTAGATACGACTATGAGGCCTTTTCTTGGTGCTTGTTGAATAGAAACTTAAATAAGGATTTGATTCTTAGATGAATATGAGCTTAGAGTAAGTCTAACTAACCAAAATATGAGCCTAGGGAACTGTTAGCGATAGTGCTTTAGTACACTTCGAAGAGGTGGCTGTGACACTTCTATGTAGGGTCTAAGATGGCAATGGATTGTATTTGCATGAAGATTGCAGTTTGACTATCCTTGATCCTTAAAGGATGATTTGCCCTTAACTTTCGAGGTTATAGAAAAAAAAAAAAGAGAGAGGAGACAAGATTATACTTATAAATCAATGATGAAAAGAAAACTGGTGCAAGACTTGGCAATATTAGGAAGAAGATAGAAAACCCAGTTTCAGAGACACCTACTTGGAAATAGTTAGTGGAAGCATTTCTCAAGGAAAACTCTAAATGATACGTTTTAAATTAATACCACTCCAAATTTCATGCCATGATTTTAAACTTTGATTGTATCAAAGCTCTCACTTGATCCTCCATCTCAATTGAAAGAAAAGCTCATAACTGCTCATTAAGAAACTCTCACATTATACATTTTTTTGGAGAATACAAAAGGAATCTTCAAGACATTTAGGAGCTTACATAAAGCACTCCAATTGGCATCATAGTTAAAGAGTCTAATTACAAAAAAATTCACAAGAGAATCCATTTTCTAGTAGCATTGTTGAATTGGAACTTTATTTTGCTCACTAGTTTCCTTGTATTATGTTTGTTTACATTTTTTTTTGTGCTTTCTTTCACTCCTTTTGGAGTTGTTTCCTTTGAACATTTGTATTTTTTATTATTTCAATGAGAAGTTGTATCTTGTTAAAAAAAAAAAGAATTCCATTTTGAAGCTAGACTAATCACCTTTCTTCCTTTCCTTTCAAGGACCTGCCAGGTTTGGAGAATGTCTCTTCGATTGTTCTTGAGAAGCATTATTGAATTCCAAAATATTTAGAACAAATGCCACCATTTAACACAAATCACAGCTGGAGATAAGTGGTCTTGAGTTTCATTTTCTTTTAGCAAGGATGCACCAGCTGCGAAGTTTGGATATTGGATGACATTTCTGCACTCTATTTCCTATTGAACCTTCCCAACAATAGACCAATGAAGAAGCTGACTTTTTAAGGACTATCTAATCTAATTGCTTGCATAAAATAGAAGGTAACATCGTATTCTTTGAGGAAGGATGTTAAAGCAAATTGTGGAAACATCTGAACTTCCAACAGCCATATTCTCTTTCTTTGCTAATGGTCATATTTCTGTCCACTTTGAGGGAGATGCACTCCAATTTCTTATCATGTAAATTCTCTAAAATCAAAGTTTAAGGAATTCTATTTTGTTTGACACCAAATATGGTTGCTTTATCTCTAATTCTAGCCAGGCTATAAATCTTCAACAAATTTAAGGCTGCTTTGTCATCCATTGATCTTACGAAAAGATGTTTCATTACCTAACACAATTTTGTAGCAGTTTTGAACCAACATTGCTGATAGAGATAAATCAGGGCATCTTAGTTTATGCTCTTTACTGTTGTGAACCACTGTCCAACTTCTTCCATACTTCTTGTTAATAGCCTTATGCCAAACCATTTCTTCAAGGGAATCTCCATAACCATTTGACAAAAGGATAAATTCTCCTTAAATTCCATTCCCAAGTCTCTTGCCACACGGTTGAGTAGCCAATTGACAGATGACATTTGTAATCTTCAACTCCTTCCCAAAGAATTCCTAAGGCTTGTCTAAGAGAATGGCCATTTTCGAGGAAGCTGAAGAAGGATAGATAGGAGGAGGGTAAACTATGGATGAGTAAATTAATAAGAGTTAATTTACCTCCTTTGAGATGTAACTCTTCTTCTGTTGTCAATTCTTGATTGAATTGTCCATTACCAGTCACTACTGGTTCCCAAAGAAGATTTCATCAAATTCCTCCAGCATTCATAGATCACAGGCCATTCCTCATCTTGCATATTAAAATTTGCTTGGCTTCACATCCTTTCAATCTATTTAATGCCATGGATAGTCTTTAAATATATATAATTATTAATTTATTATTAATCTTAATTCGGAATGAAAGGGTTCGCTTTGACCGTTAAGAGTAGGTAACCCGTTCCCTGTGTCTTTGTTTCTATTGCATTCTATCTCATCGTATCAAATTCTGTTCTGCGATATTTGAGAATCACCGTCAATACCTCGGTGTAGGTCCGGGATAATCCTTTGTTCCACAGTCTGGGTCTATTTACAACTAGCCAATTAAGAATTCTCAGATGTACTAGTACTAGCAAGTGCATCTTTGATGCAGTCATCGATTCTCCCGAGAGGTCACAATTACCGCGAGCAAACATATTAATGACGAGGAACACATTTTTGCTATTCTACTAATACTTGTACTTGCTCTGCTATTTTGCCCAAGCCTGGCTGAGGAAGAATTACGGGGCGTAAAACAAAAAAATATGCTGATTCGGGTTGGGTATACTATATTTAATTTATAACGGAACCCCCGCCCTTAACCCATTAACGAAAAAGAAAAAATAAAAAGATAAGGCCATTCCATTTCGACAAAAGACCCACACCCAAGTTCCATAGCTTTGGGTCCGCTATCCCGATCATGATTTTCCTACCCCCAGAGGGAAAAGTCCTTCCCTTTTTGGCCGGTTGTGGGCGAGGAGGGATTCGAACCCCCGACACCGTGGTTCGTAGCCACGTGCTCTAACTCTGAGCTACAGGCCCCACCCCGTCTCCATTAGATCTGTTCCCGGGAGTACCGTAAAAAAAGAAACCTTCCTCTCCCTAGCCATTTGCCATTTTAGGTTAAAACGATGTGAAAGCGCCTCTTTCTCTATAAGAACGGTGCGTTCCAAGGTGTGAAGTGAGAGAGAAAAGTGGGAGAGAAGGGGTTTCATAATTGGGGTTTTGAATAAGACGACCTTTTCATTTTTCATTTTTTTTTTCCATATTGAATATTGAAAAGTAATAAGAATTAGAGGTGTTAAGCTTTTTATCATCCTGGCGTCGAGCTATTTTTCCGCAGGACCTCCCCTACAGTATCGTCACCGCAGTAGAGTTTAACCACCAAGTTCGGGATGGATTGGTGTGGTTCCTCTACGCCTAGGACACCAGAATATCGAACCATGAACGAAGAAAGGCATGAGAGAAAAGCATATTGGCTAGTGATTGTGAGGCCCCAATTCTTGACTGGAGGGGACACCAAAGGCCTTGCCCTTCCATCCCTTGAATAAATAGAAAGGGAGGGCAGAGCTTTTTTTGGTTTTTTTCATGTTGTCAATGAGTTGAACAATGAAAATAGATGGCGAGTGCCTAATCAAATTGATCGGGTCATGTAGGAACAAGGTTCAAGTCTACCGGTCTGTGAGGATGCCTCAGTTGCATACATCACTGCACTTCCACTTGACACCTATCGTGATGATAAACGGCTCATCTCGCCGTGACCTTCTCTTGAATTCTCAAAACTTCTGTCGCTCCATCCCCGCAGGGGCAGAGAACCCATCGCTGTCTCGGCTGTGCTACCGGAGGCTCTGGGGAAGTCGGAATAGGAGAGCACTCATCTTGGGGTGGGCTTACTACTTAGATGCTTTCAGCAGTTATCCGCTCCGCACTTGGCTACCCAGCGTTTACCGTGGGCACGATAACTGGTACACCAGAGGTGCGTCCTTCCCGGTCCTCTCGTACTAGGGAAAGGTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACATAATACAGCCCTAAGTGGTGAAGAGCCGACATTGAGGTGCCAAACCTTCAGATCGATGTGAGCTCTTGGGGAACATCAGCCTGTTATCCCTAGAGTAACTTTTATCCGTTGAGCGACGACCCTTCCACTCGGCAACGTCGGATCACTATGGCCGACTTTCGTCCTTGCTCGACTGGTGGGTCTTGCAGTCAAGCTCCCTTCTACCTTTACACTTGAGGGTCAATCTCCGTCTGGCCCAAGGAAACCTTTGCATGCCTCCGTTACCTTTTAGGAGGCCTACACCCCATAGAAACTATCTACCTGAGATTGTCCCTTGGCCCGTAGGTCCTGACACAAGGTTAGAATTCTAGCTCTTCCACAGTAGTATCTCACTGATGGCTCGGGCCCCCCTAGAAGGGGGCCTTCTTCGCCCTCCACCTAAGCTGCGCAGGAAAGGCCCAAAGCTAATCTCAGGGAACAGTGAAGTTTCTTAGGGTCTTTCTGTCCAGGTAGTCTGCATTTTCACAAACATGTCTATTTCACCGAGTCTCTTTTGAAGACAGTGCCCAGATCGTTACATCTTTCTTGCAGGTCGAAACTTACCGGACAAGGAATTTCGCTACCTTAGGACCACTAGAGTTACGGCCACCATTCAATGGGGCTTCTGTCGCCAACTCCCTTGACATCAGGTCACCAACTTCCTTAACCTTTTGGCACTGGACAGGCGTTAGCCCCCATACATGGTCTTACGACTTTGTGGAGACCTGTGTTTTTGGTAAACAGCTGCCCGTACCTCGTCACTCCAACCCCCTTTGTGAGAAGGCACTCCTTGTCCCGAAGTTACGAGGCTATTTTCCCTAGTTTCTTAGAGAGAGTTGTCTCACGCCCCTAGGTGTTCTCTACCTACCCACTTGTGTCGATTTTGAGTACAGGTACCCTTTTGTTGAAGGTCGTTTGAGCTTTTTCTGGTAGTATGGCATGGGTTACTTCAGCGTCGTAGCGCCTGGTACTCGAACATTGGCTCGAGGTATTTTCTCTACCCCTTCTTACCCTGAAAAAGTAGGGGGACCTTGCATCCTTGAACCAATAACCATCCTTCGGCTAACCTAGCCTTCTTCGTCCCTCGGGACCAACAAGGGGTAGTATAGGAATATTCACCTGTTGTCCATCGACTATGCCTTTGGCCTAATCTTAGACCGTGACTCACCCTCTGTGGACAAACCTTGGAGGAGGAACCCTTAGGTTTTCGGGGCATTGAATTCTCACCAATGTTTGCATTACTCAAGCAGACATTCTCGCTTTCGCTTCGTCCACCATTGCTCGCGTGGGTGCTTCCTTCTAAGGTGGAAGCTCCCCTATCGATGCATTTTTACATGCCACAGCTTCGGCAGATCGCTTAGCCCCATTCATCTTCGGCGCAAGAGCGCTCGATCAATGAGCTATTACGCATTCTTTCAGGGGTGGCTACTTCTAGGCAAACCTCCTGGTTGTCTCTGCATCCCTACCTCCTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTTGTGATCGGGCTATTTCCCTCTCAATGATGAAGCTTATCCCCCATCGTCTCACTGGCCGACCTTGACCCCTATTATTTTGAGATCATATCTAGTATTCAGAGTTTGCCTCAATTTGGTACCGCTCTCGTGGCCCGCACCGAAACAGTGCTTTACCCCTAGATGTCCAATCAACTGCTGCGCCTCAATGCATGCCACGTGGAGGTAGAAGCCTACGCGTCATGAACTTCTTTTACCGGAGAAGAAGCAATGACGGTGGCAGAAAATGCTGATATGTATGTATTGGTATTATGTATTTTTATCGTTTTATTTTGATTTTTGTGAAAAAGATTTTGAAATATATATATATATATATATATATATATTAATTTTTTATAAGAAATGAAACAAATTCATTAAGAGACGAACACAAACAAAAGAACAACCTAAAAGCCTTAGGGATGAGGAATCCCTAAGCCTAAAAGGAGGGTTATACCAAAGGTTCCAATCTAAATTGATGAGACGAAAATGAGTGTTGGATGTTTTATATTTTAATATTAGCTTTAAATTGTAAATATTTAGTTTTCCATATTTGTTCTTTTATTAGCTGTAATTTATGTAGGTTTACCTGCCTTATTTGTAAGGGTCTCTCTACATAAGGAGACTTAGCTTTAGTAATAAAAATAAGAAAGAATATATTTATTCTTCTCAAAATTAAGATGATATCAGAGTAGAAAATAAAACCCTAAACCTAACCCTAACCCTAGCCATCAGCCGCAGTACCGCTGTTGCCGTCTGTCTTCGACGGTTCAAGAGATCTTGTTTTTCTAGTGAAAGGTATTCACTGGTTGTTGTTCATCGGCCTATCTGATCGTCTAGTCCTCTCTTTCCCGTTTGTCGCTGCCAGATGCTGGTTCTTTCATTCGCATTTGCTAGGGTTCTGCCATTGCCTTCTTTGCCTTCGCCTATCTGTTGCGGTTCCATTTGCAACTCTTTCGTTGGATTCATGTTTGAAGTTGCTAGTGTAAGGTTGCTCATCATTGCTCGCGTCTTCAGGAGCCCAAATGCTATTTCGAGGCTATTCTTTTGCTTGTGTTTGTTGTAAAGGAATTGCATTCACATTCGTAGTTTCATTGTCTGGCATCTTCAACGTGATTTTGATGAGTTCTAACAGTTTTGACGCTATTCTGGTTGTCGACGAGTTCTTCTGGTGTCCAGTCTTGCTACTCATGCTTGTTGTCGTTTGTCTGGTTTCTGTTCAAAAATCAATTTGGTTGTCTTTCATTATCGCACACTATGCTTCTTCATCGTGCATACACTAGTGTGACATTAGAGATCTTTTCGTAGATCTATTTGTTTAATTGTCTGACCCGTTCTCCATCCTTGGTTGTTGGTGTTTTATTCTGTCATTACATTCATGTGGAATAGAGAGTGGCCTCCTTATGCCAATTTTGTTGACTCAAGTATATTTGGTATAGAGCAATTAATCTACCTTTAAAATTGTCGGAGACCAATTTATCCTCTAACAATTTATCATCTTTTTTTTTAAACTAGAAACGAACTTTTCATTGATTAATGAAAAGGAACAGAAGATGTTCAAGGAAACAAACTCCCATTGGGAGTGAAAAGAGAAACAACAAAACAACCTTAAGATGAGAGATGAAAGCCCCTAATTCGTACAAATGTCACTAAAAGAGAAATTTGCAAATAAAGGAGAAAGAAAACACCAATATGAAGCTTTTATCTTGATTTGATCAAATCGTTCCTGCCAAACTAAGTGCTTATCTTCAAAAATCCTCTGATTCCGTTCAAACCAAATTTCAGAAATAGTGGCTTTCACAACATCAATCCAAATCAATTGAGCTTGAGGTTGCAAAGTAAGTCCATAAAGTAACTGAGAAATATAGTCTTTAGAATCAAAGTCAAAAGCCCACTGTAAATTGAAGAACCCGAATAGGAAGTGCCAAAAATCCGTCGAATAAGAACACTGGAAGAACAAATGATTTTGATCTTCATTGGCCAATAAACAAAGAGAACAAACAGTAGGTTGCAAAGTTGAAAATGGCAGCTTTTTCTGAATAATATCAGCTGAATTAACACCTATGAAAAGTGATATCCACATAAAAATATTGATTTTCTTGGGACTCTTAGACTTCCAAATAATCTTGAGCAAATCATCCGGCCATGGAGAAGAATAAAGAAGATGAGACGATAAAGATTTAACCGAAAACATACTCATTGGATCAATCTTTGAAATCTTGTTATCCTCCTTATTGTGAAGACTTATAAATTTCAGATTAGACAATCAAAGATTCAATTCCCCTATTTCATCATCTTTCAAATTACTACTTGTTATAAACCTCCAAGAACAATGAACATAATCCCAACAATCTGAAATATAGCAATTCGGAGAAGACACTACTAGAATTAGATGAGGGAATCTGGATTTTAGAGAAGTCTCACCAAACCTAGTATCATGCCAAAAAGAAACCGTTTTTCATTGCCTATTTGGAATGAAGAATATTGCTCCACCTTCCTCCACTGATTTGAAATGGTGAACTAGGGAGTTCTTAAACAGCCCCCAAAATTGTCGGAAGTATGCCAACCAAAAGTATCTAATCCATGAATGCTAGATACTACTTTTTGCCACAAAGAATTACCATCCCCATTTAGCCAATAAAGCGGATTTTTTTTAACTTCAATCCACCAATTCCTAAACCTCCTTGATCAAAAGGTCTAGAAACCAAATCCTGTCGAACTTAATGTTTCACTTTGCCATATGCATTTCCTTTCCAAAAGAAACTATTTATTTGCCTTTTGATCTCAAGACAAATCTTTGTAAGCATAAGAAAAAGAGACAAAGGATAGAACCTTCAATTATTGAGCAGCAATACTTCATTTAACTGATTTATTTGAACTAATCGCATTTACTGAAATTCCACAATCCTCAAGATACTTAAAAAATTTTCCTATGCCAGAATCTAATTTGGAAGATGGATGTTCAATCAGCCCCACTGGAGAAGACTCAATAATGGTTTTTTTGAGATTTAAGTCATTAATGATGGGGTCAAGGAAACAATCAGCCTTTTCTAAAATGGAGGGTTATTTACTTCTTGGAAAAGAGAACGCAACACCTCTTCATATGACTCTTCAATAATGGCTTCATTAATGTCATTGAGATTTGGGTTGCTAAACTCAGATTCCATGCTACTTACACTTACATCAGAATCCTCAATTAACTCTTCATTTTCAATTACTGAAGAAAATTTGCTAAGGTCTCCACTAGCTATACCAAAAGACAGCTTGTTGCCCATGGAGTTGGGAGATTCCCCAATTAAATTACTAGTAGATGGTTTGGCTATGGACCATTAATTGCTTCTTCAAAGACATGCAATGCTCACCATTAAATTCTTCCAACTGTACGCTTTGTTCAATTAAAGGTTCATCCAGAAAACCCAAAGACTTCATGCATCTTTCCTAAAAAATGTCTGTATTAGGAATATCCTGTTAGGCCCCCAATTGTAAATACTTACACTAGGAGGGGTAAAATGGGAAACTCTCCCCAGACAGTTAGTTAGTGGGAGAGTGAATGAACGTATTTGAGCAAGGGAATGTTGTAGGGGAGGGATTAGGCAAGTTAGCAAGATAGCAAGGGTAGAGGAGAGGTTACCAGCCCTCTCAAATTGGCTGCGTCGTTTTATCTCTCTTTTCATGAACAGTCTTGTTGAGTTCTTCTCTGGAAAGTTGATTTTCATCAATAGAACAGTAAGGGGATACCTTACATATCCTTATTAATGTCATCAAAAAATGAAGAGGCTAAATTTTCCTTAATTAATGCATTGATTCCTTTCCTACGAACATAGAACAAAGGATATACAGCATTGTAGGAATTCTTTAAATCCAAAATGCTTTTGGACAAAGGAGAGACAACCTTTGGGTTCTTCATGAAATATGAAGTAAATTCCTTATTAATAGCAATTTCATTACTCAATTGATTTCCAAGAAGAGAGTCGTGCATGAGAGAGTCATTAATTGAAGTCCCACCTACTTCCTTAAATTGATCAAGAATTGGAACCTCTTCATCATCAAAAGAAAAAGGGATACAAGTAGCCGCTTACTCTTCGTCTTCCAAAACTGTTTTGTTCTGATCAAGATCTAATTTGTTGGTAGTCTTACAAGAGACAAGGCTGTCCTTACATGCATTTGTGAAAGAAGGATGAGCAAGATGAATATCAAAAGCATTGCAATCCGTAACTTGAAAAAAATACTACGAAAGGAAGGATCTTTAATCTCAACCTCTGCTGGAAGGAAACCATAAATGTTTCACGGAACCTCAATGCAAACAGAGCTACAATCTAACATATTCAAGGTCTTGGAAGAGCATTTAACAAACCCACCAAAATAAATCCCTAAAGCTTCAAAACAGTGACGATTCCAGAATCTCAAAAGTAAGTTCTGAATGTTGATCCAATCACCATATCCCACCACAAATTCAAGCTTGGTATGCTTTTCTATTGACCATTTCTCAACCTTCAAATGAAAAATACCAATGCAGACAAACATAAACAAACAAACAAACACAAACAAATAGGTAATTATCCTAAAGCCTTTTCTTGTAATTATTCATCTCCGTGGATTATTGATATCGGAGCTTCTGATCATATGACTAGTTCTTTACATTTATTTGATTCCTACTCTCCTTATTGCAATGAAAAAAATTAGAATTGCATATGGCAGTTTTTCTCCTATTGTAGGAAAAAGAACTATAAAATTGACTGAGGAAATTACTCCATAGTCTGTACTCCATGTTCCCACATTAGCCTGTAATATTTTATCTGTTAGTAAACTTTCTAAAGATTTGAACTGTCATGTTGTTTTCTTTGAGTCTCATCGTATTTTTCATAATCATGACTCAGGGAAGATGATTGGACAAGCTAGGATGCTTGGCAGTCGCTACTACTTTGATGAAGGTTCTTCTCCTAATAAAATAACTCAGGGTTTTAGTAGTATCAGTTCTTCTGTTAAAGAGCATATTATGCTCTGGCATTTTAGACTAGGACATCCCAGTTTTTGTTATCTCAAATATTTATTTCCAAATTTATTTAAAAGAATTGATTGTTTCTCCTTTCATTGTGAAAACTGCATTTTAGCAAAACATCATTGTTCCACATATTTTCTCAAGTCTTACAAAGCCTCCAAACCATTTTACTTAATTCATAATGATTTTTAGGTCCATCTAAGATTAGGACTCAGTGGGAAAAAGATGGTTTGTTACCTTTATTGATGACAATACTTGTTTGTCTTGGGTTTACTTAATGACAAAAAAGTTTGAGGTGAAAGATCTTTTTAAACATTTTTACAATATGATTGAAACTCAATTTCATACTCAAATTTGCATTTTTCACTCTGACAATGGAACTGACTATTTCAATGAGTATTTGGATGATTTTTTTAACGATAAAGGTATTTCTGATCAGTCCACTTGTTAGGATAGTCCTCAACATAATGGGATTGCTAAAAGAAAAAATAGGCGTTTACTTGAGGTTGCACGCGCCATAATGTTTTCCATGCATGTTCCTAAATATTTATGGGGGGAAACAGTTGTTACTGCTGCCTACCTTATAAATAGGATGTCTAGTAAAGTCTTGAATTTTAAAACTCCCTTAGAGTGTCTTAAAAACATTTTTCCAACTACTCGCATATATTCTAATTTGCTTATCAAAGTTTTTGGGTGTACTACTTATGTTCACATACCTAGTCAATTTCGGTCAAAACTTGATCATCAAGCCACTAAATGTATTTTTTTGGGTTATTCTTCAAGTCAAAAGGGATATAAATGTTTTTATCCTCAAACAAAAAAACTTTATGTGAGTATAGATGTATCCTTTTTGGAAAAACAACCACATTTTACCCTAAATTGTCTTCGAAGGAGACACCAAATGTGGAAGATAATTTTTGGGATGTTTCTATTCCTCTTCATAGTGTTATTTACTCTGATCCTACTAGTTCTTGATGCAAGTGTAGAAACGGAACTTTTCATTCAGGGGGAGAAATACTACAAAATGATTCCACTAATCAAGTTCCTGGGTTTTAAGTTTTATACTAGAAGATCAATCAATCAAAAGAACGAGGACTGGATAGTTGACTTGTCACAAATCCAGTCAGACACTCCAAAGAATGACACTAAAAATTTAGGTAACCAATTTTTTAATCCGATTCCTTCAACAATTAAAAATGCCTTACCTATTGTATCTGATCTTGATGTCCCTATGGCTTTGAAGAAAGGAATTCGAAACTGCACCAAATATTCCATTGCAAATTATCTTTGGTATCAGAAATTGTCCAAGAGTCATAAACTTTTACTTCTAAGATAACCAACTTGTTTGTTCCAAGAAATATACAGGAAGCCTTAGATGATCGAATTGGAAACTAGCAGTTTCAGAGGAGATGAATGCTCTAAGACAAAATTGCACTTGGGGAATAGTAGATTTACCAAAAAATAAAAAAACGGTGGGATGTAAATGGGTTTTTACCATAAAATGTAATGCTGATCGTAGTATTGAGAGATACAAAGCTAGACTGGTTGTTAAAGGATTTACCCAAACATATGGAATTGATTATAAGGAAACGTTTGCTCATGTTGCTAAATTGACTCTAATAGAGTTTTACTATCTCTTGCTGCTAATTTGGATTGGTTCCTTCACCAACTTGATGTAAAAAACGCTTTTCTCAATGGTGATCTTGAAGAAGAAGTGTTTATGAGCTTACCACTTGGTTTTGAAATGGAACTGGGGAAGGACAAAGTATGCAGATTAAGAAAATCTTTATACGACCTCAAACAATCTCCGAAAACATGTTTGAACGGGTTGGAAAAGTAGTCACTAATTATGGATTTCTTCAGAGTCAAGCTAATCATACCATTTTTTATAAACACTCTGGAAATGACAAAAATTGCAATTCTGATTGTTTATATAGATGACATTATTCTCACAAGGAATGACGAAGTCGGATTGGCTACTTTAAAGAAACAACTTGCAAGTGAATTTCAAATCAAGGACCTGGGAACATTAAAGTAATTCTTGGGAATAGAGTTTGCAAGGTCTATGAAAGGTATTTTTGTTAATCAAAGAAACATATTATTGATTTACTTCAAGAGACAGGTGTACTTGGCTGCTGGGTAGCTAAAACCCTATTGAATCTGATTTGAAACTGGAAGCTGCAAAAGAAGAAGAGCTAAAAGATAAGGAAAAGTACCAAAGACTTATGGGAAGACTTATTTATTTGTCTCACACATGACCAGATATTGCATTCGCAATGAGCATGGTAAGCTAGTTCACGCACTCACCAGGACCAGCTCATTTTGAAGCAGCTTGTAGAACACTAAGATATTGAAAGGAACTCCAGGAAAAAGGTATATTATTTAAAAAGCATAATCACCTACATGTTGAAATTTAAACATATGCTAATTGGGCAGGGCAGGAAGTATTGTAGATAGAAGATCTACTTCTGGTTGTTGTTCCTTTGTTAGCTGGAACTTAGTTACTCGGCGAAGCAAAAAACAAAATGTAGTGGCAAGGAGTAGTGCTGAAGCAGAATTTAGGGCTCTAGCCCATGGTATTTGTGAAGGCATATGGATAAAAAGACTGTTTAAAGAATTGAAGCTTTTTCATGAGACACCTATACGTATTTATTGCGATAATTAGGGCGCCATCTCTATAGCTCATAATTCAGTTCTACATGATAGGACAAAATACATTGAAGTCGATAAACACTTTATAAATGAGAAATTGATGCTGATATGATATGCATTCCCTATCTTCCAACGATAGAGCAAACTGCCGATGTGTTAACGAAGGGACTCCCAAAAGTACAATTTGATAAATTGAATAACAAGCTGGCAATGGAAGACACCTTCAAACAAGCTTGAGAGGGAGTGTTGGATGTTTTATATTTTAATGTCAGCTATAAATTGTAAATATTTAGTTTTCCATATTTGTTCCTTTATTAGCTATAATTTATGTAGTTTTTCCTGCCTTATTTGTAAGGGTCTCTCTCCTACATAAGGAGATCTAGCTTTACTAATAAAATAAGAAAGAATATCTTTATTCTTCTCAAAATTAACAATGAGTATTACAAAACGATTTGTGAAGAGCACTCCAAATAAAGGTTGTATACCATACATGATCTTTGAAATTAACAAAAGAAGTGAATTTTTTGAAGAAGATTCTGGAATTCCTTTCCTTCCAAAGTAGCCAAAGAAAACTCCTAATAGCATTAGACCAAAGCACCTTAGTTTTGTCATTAAGCCACCCATTGAACAAAAGTTTTGTGATACAGTCATCTATTCTTTTGGGAGGGCACCAAGCATAATAAAAAAGATTGCAAATGAAATTCCAATCTTTGCTAGCAAAAAAAAACATTGAAGAAATAAATGGTTTTGAGGATTATCACTGTTACCACACATATTACAGACTTAGGAATAGTGATGTGAGGATTGCTTTTTTTGAATCTTATCTAAAGTGTTGAGGTCACTATGAACCAAAGACTAGATAAAGAATTTGGTTTTCTTGGGGCTAGGGCCTTTCCAAATGAGCTTGCTAAGGTTCTGGTCTAGGAAGTGAGCATCTTGAGAAAGATTATTGGTGGTCGACTTCACAGAGAAAGTCTCAATAGTGTTAGGGGCCCAGAGAATTATATCCTTGAATTGGCTCAGGGAGATGTTTTGAAGCATATCGGCTAGGCTGGATCAATCCTCCATTTCTCTTACTAATAGCCCTTCTCAAATGACGATTCCAAGCGTTGCTATCTTTAGACCAACAACTATGAATAGAGAAGAACTTGGAGTTAGCAACCACATAAAGAGAAGGGAAGTGCCAAGAGGCTGTGACGTCACCTAATGATTCTCCCAAAATCTAATCTTTCTCCCATCACCTAGCTTAAGACGGATATTCTTTTCAGAAAATGTTTTGGTCTTCAAAATACCTCTCTAAGGGTTGCAAACACATTTGACATACTTTAGTTTGGACAACAAAAGAAAAGACTTCAGGCCGAATTTGCTGTGGATAATTATAGAGCTCTTGAGGGAATTGGATTTATGCATTAGGCCTATCTCAAGGAAAAACACTCCAGGGGCGGATCAAGCAAAAAAAGGAAACAAGACTACATAGAACTAAGAAGCCTAATAAGCTTCTAGGAGGAAAATGATTAAGACACTCAGAATTCACATCCTAAGGGCAAATCGGTTTCGTTATGAAGATTATCTCTTGGAATGTTAGGGGTTAGTGAGCTTGATCCAAGAGACTTTTAGTTTAAAATCTTCTAGTAGTTAATATCTCAGACATTGTCATTTTGCAAGAAACAAAGCTTGACTAAATGTTTAGTAGCTTATGAAAGAGATCTGGAGCTCTAAACTTATTGGGTGGCCTTCCCTCGAACACTCAGCCGGCAGTATCTTGATTCTGTAGAAGGAAAAATTAGTGACACTAGTGGACTTCATTATAGGCTCCTTTTCTATGTTCATCAAAGTGCAATTGCAAAACAGTCTTTTGGATTAGATCACTGGGTCTACTGGCCTTCCTCCTTTGTGTTAAGGAAATTTTTTTTGGAGGAAGCTAGCGATCCTTTTGGGCTTTCTGATGATTCTTAGTGTGTTGGAGGCGATTTAATGTCATTAGGTTGGTAGCTGAGAAATCCCTTTTGGGAAAAATTACCAGAAGCATGAAATTATTTAACAAGTGGATAGAAAAATGCAGCTTATTTGATATGCCCTCTCTAACTGTGCCTTTACTTGATCAAGTATGAGGTTCCCCAACCTTGTCCAAACTTTACAAATTCTTTGTTTCATCAAGTTGGTTGGATATCTTTGCCAATTCTTAGGCTTCCAGATTAGTTAGTAATGCCATTGATAGTTGTCTTCTTCATATTGACATTTAACCCCAAAGTGCTTCTGAACATCATAAGGATTTTAAAGACGTTACTCAACTTTTCTTCGTTGTCATTACAAATATGATTGATTGTCTAGAAGCTTGTATAGAAGTTAAAAAGAAAAGAATTTTTGTGGATTCATCCTTGCTGAAGTAGTCATCGAGTTTCCAAGAGATGGTAGATTTGTTATGCAAGTTGTGGTGACTTATTCTTTGAGATTTCTGGTGGGAAGAGTACAAAAAGTTCATGTAGCTCCTTCAAATGAGATTGCGGAAATAATGCATAAAGATCTATTGTCAATTGATGCATTCAAACTAGGTTGTCTATAAGGAAGAAAATCTGATATGTGCGAAGAGAAAGATGAACATTGTAAAAAAGGGGAGTTGAATGGGTGGGGTTGAAACTTCTATTGAAGATGAAAGGTCAAATTTGAATCTTAAGAAAGTCGAAATGGTGGATAAAATTTTTGAAGAAGGAAGTGAAAAGGCAAAGGTAGCTGGAATTGAAGTTGTGGAAGAATATAAGGGAGCTGGGAGGCGTAGAAAGATCTAGGCAGTTGTATGGGAAAAGGAAAGTTCGGTGAAAAAATTGCACTATGTTGGTGAAGAAGGCTTTTGATCTCATTTAAGGAGGAATTTGAGGCAGTTTGATAAAATTATTGATAAGCTCTGGCTTAGGAAGACATCTTCAAATTAGCTTGATGGCATTCGATAAAAAAAAAAAAGAAAAATTGGCTTGATGTGGAGTGTTGAATTTTCCTTTATATTTGTTGTGAATGTTAGTGGTCCATATTTATGACAAAAATTATGTTATCTTTATTTTTAGTTTTTTTTAGATGGTCTTTCTTCTATGTGAAAGGACCTTGTACTACCTTCATAATATAGAAGAAATATTATATTCGTTTCATCACAAATTCAAGTGAGGCAACCTCTCCCCAATAGGTTTAGGAAGATTCATTTAGAATAAAGAACATCTTGCTACTTCAAGTAAATGACATGGTTTTCCTGGTGGTAATCCTATTTTGTTGGGGGTATCAATGCAAGTGGGGTGATGAAGAAGGCCTTCTTCCAATCCATTAATTTTTCATGAATTTTTCCTAGTAGGTGTCCATCATGTGTCCAATTTTTATGCAATGGTTGCACCACATCTTCCCTCTTCTAGATTGAATTTCTCCCCAAGATGGATGCAAGTTTCGAGCATTGAATTTTTAGTCTCAATAGGTTTGGTAGTTGTTCACGACATTTGCTTCCTTCTACTTTCTTTCCTTCTTTTTTTTTTCCCTTATAGGAAACAAATCACCTTTTTACTTTAGCAAAAACGTCACAAATAGTAGAGAGTAGTTTGATTGATAGAACTTTATCTCTACTTCATCCAAGTTCTTGTTCAAACCAAATAGAAACTTGTAGATTCGTCTTTTTTCCACAATTTTTCTATATAGGCTGCCGTTGGTTGTACATTCCTGATCTTTAGATTCGTTCATTTCTTGTTGTTGCTCTACTGAATGAGGAGGCTGATATTTTGGGTCACTAGTAGCTCCCTTTGCTTAAAATCATGTAATCATCTTCCCTCAAAATTCAAATGAAATAGTTTTTGTAGTTTTTTTCATATCTAAAAAAATCTCCTTAACACGGTCTTAGTTTTCTTTTGTTGTTTGGCATAAAAAAAATTCTCCAATTTTAAGTACTTATTGAATTAATCAACCACGACGTTACCAAACTACTGTCAACTTTTCGTACTCAAACTTTTAGGTCATCATTCACTGGTTGCTTCACTCTTCTAAGTAGGTAATTGTCCTTTTTTTTTCATTTTTTTAAATGGTAGATAATTCTTGCCACTCAATTTGTAGTTTGCGATTTGAAGCTTAGTGTTGTTACTATGCCCACAGTTTCTATTCTCGTTGGAGCTTTGCCCAGGGATTGACTCTTCCTCTGTGGAGGTTGATATTTTGGTGGCCATGGATTCAGGTGTGGTCCTAGATGAGTAGAATTATTGCTTTGATACCATGTAGAAAGAGAATGAGATAATGAGATAAAGATGGAAGAGAACAATTTTCATGTATTGTATACATTTGGTAGAAATACAACCCTATATACTTTTAGTGGGATTTCTAACTGTAAGTCTGTAACTAAAGGCTGGGTTATGGGCCCCAACATGAACTTTTGTAGGAAGGTCCTAAGAGGCAAGGAGTCTGCCATCAAGTGAGCTGGAAAGCCGTTACCTTCCCTGTTACATTGTTACTTGTGGAGCTGTAAACAGTGGTATTATTAAATAGAGAAAATATCTCTCCTATTCAAATGGCTTTGGAGGCTCCCTAAAGAAACCAGCTCCCTATGTCGCAAAATTATTTGTAGCATCTATGGAAAGGATTCATCAAATCAATTTTTGGGTTGTGTAATTCTTTTACTACAACTAGAAGCCTATAGAAATCTATTGCCAAGCTTAATTACCTTTTCAAGCAGTTTCTGATTGTTGAGTTAGGAGATCAAGTCCCAATTTATTTTTAGAAAGATGTTTGGTACAAAAATACAGCTTTGTGTAATGATTCTTTGATACTCAATGCCCTGGCTAATAGCAAAGAAAGGTGCCTCAGTTAAGAGTGTTTGGATGTGGCTGATGCTAATTGGGATCTTAAGCTTAGCAGGTGTCTTATGGAATGAGACCTTGATGATTGGGCTACTCTTTATCATAGACTTGAGAATGTCTGCCCTTCCTCAAATAAGGACATGTTAAAATGGAGGTTAAATATCTAGGGGTCTGTTTGGAATACTAATAGGGATATATTAAGAATTTTAACATGGCATATTTGTAGTTACTTGAAAAGTTTGTAGAGTTGAGTCTATAAATAGAGTGAGTTAGGGGGGTATGGAGGTAGGTAGAATTTGGAGGGTAGTTTTTGAGAGAGTCCAAGCCCCTAAAAATACTTGGGATATTGTAATTTTCCTAAAATATCCCTATATTAGTATTCCTAACAGGGTTCTACTTCGTTAAATGTCCTTTTGATGTGCTTGCTGCCAGGAAATATCTTAAATTTGGATCTATGCAAAGATCTAAAATACCAAGCTGCCTACGAAGTGTATAGTTTCTTTTTTTGTGGTCTGTTTGCCTTGGGGATATTAATACGATTAATGTGTGCAAAAGAGAATCCCCTTTAGAGACATATCTCCTAATTGTTGTGTTTTGTGCAATAGCCAAAATGAGACTGCTGCACATTTTTACGGACTATTTATTTTAGCCAAAATGAAAGTTTGGTGGTTTTTTTTTTTTTTTTTTTGTATCTTTGGTTCCAATTTTCCACACCAAGTAAGAGAAAGGAGTATATTACTCAACTTTTGGGTGGTCATAAATTCAAGTCCAAGAAATCCTATAGTTATGTGGTTCTTTTTACTTTTTTTTTGGGAGGTGTGGTTGGAGAGAAATAAAAAATCTTCTAAGGTGTCGAGCATTACTGGGAGGATGATTTGGATCTTACTATTTTTGTAGCTTCCTCTTGGCAATCTTTTCCTAATTATTTTATTTCTATGGTTATAGCCAATTGGAGGGCCTTTATCTAATTCTATGGCGTCTTTGTTATTTCTCTCCTTTTTAAAAAACTAGAAAATTTTTATATGCATTGCACGTGTGATGTATTTTAACCTATGTCTTTAAATTCTAAGACTACGATGCTGTGTTTTGTTTTAAAAGCTATTTTTTAAATTTTAGTCAATTTAAAAAACAAACCTTCGTTTTTTTAAAAATTTTTTTAAAATTTAATTACGATTTCATGAACATTTTTAATTTAGCGTGATTCATTTTAATTATAGGACATAAATATTATTTTTAATTTTAATAAATGTAAGGGAATAAATAAATCATAAACTATTACTATTAGGAGTAAGTTTGGAATTTAAAAGTATATAAAATATAAACATGTCGAGAAATGACTCTCAATTATCTTAACTTTTAACATAGTAAAAAAAAATTGTATAATTGATTAATCAAATGAAAAAGTATTTATATTAGTGGCATTATTGAAATTACCAATTAAAGAGTTGGGGTAAAATTGTCCAAAAATAATTCTCCAATATTGTACCTTTTAATATAGTAATGATAATCCATTTAATTCCCATTGACTTCATTTTTTAAAGAACTGTTGGTAATTATTTTGATTCCCACATTTTGCAATTGAAGAGCATTTTTTATCTCCTTTGGGTGCTTGGATGCCTAGGTGGTTTCCTTATCTATTCTTTTTGTATCCTTTTATACTATAATGAAAGTTCTTTTCGATTAGAAGAATAAAAAATAAAAAAAAAACATTTAAAGTATCGAATTATGTTTATATATTAATATAGATATAGTATTTGCTCTCTATTTAACTTTTCAGGAGTTCTCAGAGTTCCAATGATATTGTGTTTGCAATATTCAATAACACCAAAATATGTTGCTTTAGGTCTTGCTTTATTACAGAGGCACGACCAACAAGTACAATCTTCTGAAGAGCTCATGGAGGCACCTATAGATAAAATTCAGGAGGCTGCTGTTCTTCATAAATTTGCTGAAGCTGCTTACACTGTATATCAATTGATTTTTTTTATTAGGTTTTTTGTCTTGAGAAAACTACATCTAAGCCATGTGGTGCAGGGACCATTGCTTGATTTTGGAAGAAACCCTTGCTCATTTCCTTGTGCATGGCTCTATCGGCAAGGGATATTGACTCCATGGACTCGTAACAAGTAGTTCCTTTATCTAGAATGTTGCTCTAAGCAATGTGTACTATACATGTATTCTTCTATGGTTAAAGATATTCTGATTGCTTATGTTTCTCTTTCATCTTTTAACATTTTTTCCTCCATTTTTCTTATTTCCATTTTAAATTTGTTTGCTTTGTTTTCCACTTTTGTAGGCAGCCCATACTCCATGGTGATAACTGGTGGCGAGGCCATGCTGCAGCCTTTCTTAAATATGTCAAGTTATCACCTGAAGTACTTCGAAGAGGACGTGTTAACCAGGTGATTCTGTCTTCCACATGAACGCATGCAAATTATTCAGACTTCAGCCGTGACTGGCCTAATAGTGTTTTTGGTTTAACTTCATTTATTGTTGCTCTTATTTATTCATTATTTCATGATTATGTTCAGATTAAGAAGCGTTTCTTATAGCAATTGAAGATTGTTGAATGACGTGGTTGCCATATATGAAGCACTATGTTCCTATGTTTTATGTTCTCTAACTTTTTTCCTATCTTATTTATTGAATTCCAACATTTCAAGACTTAGTATGAGTTTATGATTGGTTTGGTTAGGTTGTTGTTGCAATCCCAGGGGAAACAATAGGTTTTTTTTTTTATAAGAAACAATTTCATTCATAGAATGAAATAAAAAAGGGCATAAAACCCCACCCAAATGAAATTACAAAAAACTTCTCCATTTGGATAAAAGAGAACTCATGCTATAATTGCAAAAATGAGAAATATTTTTACACCAAGATAAAGCCACAAAAGATAGAGATTCAAGGAGGTTTTTTAAAGACTTTTCCTTTGATAAAAACCCGATCTTGCAATGGAAATTGACGGAAAATAATACTAAGATTGTATTGCAATATAGAAAGGAAAATTACAATATCAAACCTCAAGCACTTCAAGAGGCCTGAACTCTCCTACCCAAGCACTTCGAGAGGCCTGGACTCTCCCAAAATTGCTCTTCTAAATTCTGTCTCCCTCTCATCTTCCTAATTGCCCTATTTATAACTACTTCACTCCTAAGTAATTATCATTATGCCACTACTAATATCCTAATAATGCTCCTAATATATCCATACTAATATTCCTAACACTACTCTGCCCCTAAAACACCTTGTCCTCAAGGTGTGGGTGATATATAACAGCTATAGTGAGCATTAAACAAAAGAAACCCATTCTCAAAATCCAACCCAAAGCAACAATAAACACGAACCCAATATTCAAATTCACAATTTCTAGCCCTAACGTAATAATAATGAACGACGGCCAGAAAACAACACAAAAAAACATGACATCCTGGATTTTCACAATGCTGCTTCCTTCGTTCCTAACCCGCCATGTCCAGCCTCCATCCTCATCTTCCACCATAGATCATTGTACCCAGCTCCAGATTCTTCAAAATTGGTTTCAGTTCCATCGAAATTAAAAAGCTCACTCCTAATTATCACACACCCAAATGCAGCTACATTCCTCATAACATTTAGTAAACCCAAAATTCCATCTGTCGGCTTCTTCAAATGGTAAAAAATTTCTCGTGCTTGCAAAAATCGAATCCCTCCACAAGAAATGGTTGTAATTTTTCCCGAATCTTGAAATCCAATTCTCTTCCCATTTTCACACTACAAATCACTAGGCAGCTCCTCCAAATGTGTGTTGGATCCACATGCATTCCACGAACAATCCCAACAGCCTCCATTGATTTCCCTTTTCTTTGCTGGAATTAAAAAACTCATCCACAACCCAAATCCTCCTTCACCACCGGTGATAAAAATGCAGCTATACCTGCTTGATTTTGTGGTCAGGTTTACTTGATCAGCCCCCCAGAAGCTATGCACGCCTTTTGTCAGCGTTTTTGGAAGCGCTTCCTCCTCATCAGTCCACCCTTGAGAAGACGCTGCCCCAACATTGGAAGCGCTGTTGTTCCACGACTTTGATTAAAAAATTAGTTCAGCCGGTTCATACCTACAACTTGATCCATATAATTAATACCCCATTGGGCCATTATTTTTCTTTCAAGCCTACTTACTGCCTGCTGCCTAAAACCTGATGTTGGCCCACCCAAGACAGTCAATGTGCTTCTCCATTTTAATCCAGACAAAATAGGATATATACAAGTTTTATGAAATTAGACCTCTTTAAGTCTGATGGGCCTAGGCCTAGTGAATACTCTTCCTCCAGTAGGTCTCACCCACTGAGTCCCTAACCCTATTCCCTCAACTCTTCTTATCCCATAAATTTTATCCTTATGTCGCCGCCACCGCCCCCTTGCAATTCTTTGCGATTTCTTCTGCCACCGCCAATAAACTTTCTGTAGAAGTCCATGATTACACTATTTATTCTCATATTCCAAATACTCGATTTCGCCTTCAACTTTCTTGCGGAACTTCCAGCACTGAAATCGTCTGCACAGTCATCACTTTCTTTTTCTCCTCTTCGGCCACCCCACCTTCGGTTGAAGACAACTCTCCTTGTAGACGCCCAAGAGTTCCTTGTACGCAAAAGGTTTCAGGCTTTCTTAAGTGGTTTTCCATTGCAAAGTCATAGGTGTTCATTGGTACGATCACTGTTGTCCAGCCACCCTCCACCCCGTAGCGTGAGCGTCTAATTTGCTCGTGGTTGATCCAATCTTGTTGAACTTGCAGTTTTGAGTTTCTTCTTTCACTCTGACCAAAATTGATTCCTCTTCTTTTAATTTCTGTTCTCTCTTCTTCTTTTAATTTTTGTTCTGTTTCTGCCTTCTACATTTTGAATCCTCAACCCATTCCTGCATTTCTACTATAGCCGGTCATATTGATTCCAGAATATCTTTCATTTCGCCGCCAATTTATGTAACTTTTGCAGTAATTCTAAATCAAGACCTATGGCAAGAATCAGTGCTCTAATACCAATTGATAAGAATCCGATCTTGCAATGGAAATTGACAGAAAATAATATTGAGATTGTATTACAATATAGAAAGGAAAATTACAATATCGAACCTCAAGCACTTCAAGAGGCTTGAACTCTCCCACCCAAGCATTTTGAGAGGTCTGGACTCTCCCCAAAATTACTCTTCCAAATTCTGCCTCCCTCTCATCTTCCTAATTGCCCTACATTATGCCACTACTAATATCCTAATAAATAATAATGCTCATAATATTTCCATACTAATATTCCTAACTATCTAATATTTTTAACATCCTTGTCTTGGAAGATTCTGTAATTCCTCTCATTCCAAATCGACTAAATAAAAGCTCTATAGAAGTGAAGCCATAAAGTCTTCTTTATGTAGCAGAAAGGGAGGGCTAGGGGAAACAATAGTAGTCTTCGTCCTTGTAAGTATATTTCTAAGTGAATGCCAACAAGTTTTTTTAGAAAAACCCCTTAATGTATGTGACATATTCAATTTTGAAGAAGTGTATTACTTCCATAAATAAAAAAAATGGACTTAATGATACCAAGAAATTGGCACTACTCTCCCCCTTGTTCTGTCTCTCACCACTCTCTCCTAGTAACCTCAACCTCCCCTGTTTGTTGTTTAGCTCTTGTGTATATTCTACGTCTGCTGGAAGTGTTCTTGAACTGGTTTTAAAGGTTATCTTTCTCTAGTCATCAATGGCATTCCACTCTCATCTCCCCTGTTCTTGTTTCCTTAAGGAAAAAAAAATCAAGTTATATTTGGATGTTCATTCTTGAGGTTATCATTTTCTTCTATCAGAGATTGGTGCTTACAAATCATTCTCAATTTCGATTGAACCAACCTCAGTTGGTTCAACAGTGTAATGAAGAATCTTATTAATATTCCTAGAATTTACAGATTTTTCAGAGAATTAAAGGAGGAAGATTATATGCTTTGGGTTGAAAAAACTTCAAATAAAAAAGGGTGCATTCCTGAATTTTATACACTTGACGATTGAGGAAGAAGAAAATGTTGTTTCATGGTTCTAAAAGGTTATGACAAAGGTGGTTAGAAGACTTTTCTAAGCATGCTGGTTGGCAGGCATGACAGCTTGGACATGTCAAAAGCCTCATCTTCTTCTTCTGTAACAACAACAACAGTTCATCTTCAGGCCTTATCCTTTCTCTATGCAAATACTAATTGTGGCAACTTTGGTCCTTCAATGGCCTATCAGGATATTAAACTAAAATGCTAGCCTTTTGTGAAAAAAAATTTATGATGAGTCTGAGATGGGCTACCATAATCTTAAACTAGTTTCTGATTCGGTATAGCTTCTTGTCAGCTGCATATTAATCATTTTGCTTATCTCTTCATATTATTTTTGCAATCCAATATGGAAGATAAATATTTTAGAAGATTTTAAAAATTCCTTTTTTTATCAGTTTGTTTTTTTTTTATAACCCTGTATCGAGGGCCCCACCCCCACTAATGCAGGGGGCACCCGCGCCCGCCGCAAGGCCTGGGACATTAAGGGTTTTTCTTATTAAGCTCATCCGAAGATTCGAACACACGACCTCTAGGTAGTTGTTATTTAAAGATCGCGTGCCTTTGCTAACGGGGCCGCCTCTTGGGGGCTTTTATTAGTTCGTTAATATTGCACAATCATTTCTCAACTCTAGTTATCTTTCTAAGCTTTTGGTGGTTTATCTCTTTTTAGGCAAAGTGTGAAGCTGCATATTTCGTTCTGGTTCTACATGATGTGAGATGTGTAGTGATTGCAGTAAGGGGAACTGAGACACCTGAAGATCTCATAACTGATGGTTTGTGTAGGGAGTGCGCACTTTCCGAGGAAGACTTGGATGGGCTGATTAAGTCTCTTTCTCTCTCATACCCCCTCCCCCAACACACAAAATACACACACATTACATTTGGTATGTATTTTATCCAAATGGCATAGGTTTGGAACTTATCAGAAAGAAGAATTTGAGAATACTTAGAATAGTAGAAATTGTTTGGGAAAATGTGACTGAAGAATGTTAAGAAAATGATAAATGAGTTTGATATGTAAGGTAACCATTGGATCACCTAGAGCTTACCTTTGAATTGATTAAAAAGTTAAAAATCGAACGATTAATCTCACTGGATCTCAATTAGATGTTTACCTCGTACAGAAGGGGAAATCGGCAACAAGCTTACTATCTTATTATAATTTGGTTTTAGCTGGGTTTTCTCTTAGTTTTCAACATGCCAGATTAAACCAATTGCTTATTGATAGAAAGTTCTACTGAAAGCTCTGTGTTTTGTAGTTCCATCTTCAATATTGATTCGCCATAATCATTAGTTGCTTTTATACAGATATAGTGTCTTAGTTGCTTCCGAAGTTTGTTTCTAGCTTTTAGCTCTCAGGACATATAAGTTTTTCATTTAAAACCTTTTCCATCCTCTGCTTGTTTGCAGTAACGATCATGTTCACCCCAATGTGAAGCAGAAGATTAGATCATCCTTCCCACACTATGCACATTCTGGGATTGTTGAGGCTGCCAGGGATTTATATATGCAAATTGAAGGAAATTCTAGAGATGATGATGGTAGGTTGATGTTTTTTTATTGCTGCCCTTTAAACTTTCTGGCTTTTATTGCTCCCCATTTTATTTTGTCATTGGTACCTAGAAATCTGCTTTCTTTATGTTTATATAGAAGTTTGCGACAATTGCTTAATGCTACAATCGTAGTTTAAGTCTAGTTGATGTTTCCGTTTTAACAAATGTCTTTATGGTTTATATTTGAAGGATGTTTTCTATTATGATTGATAAATTATCACTTAACCTAAAAGCTTAAGCTAATAGGTTACTAATTAATTTAATCTTTTATTTATATTCTTAATACTCCCCCTCAATTGTAGTTGTGAGCTTAGAAATTAACACAAGCCCAACAAGTCACTATCAATATCAATTAAGGAAGAAATAAAATTACAGGAGTTTGAACTACTCTGATATCATGTTAAATTACCATTTAACACAAAAGTTTAAGCGAATAGATTATGATAAATTTAATTTTTTATTTATGTTCTTAATAATGATTAACTCTTTTGCTCTATATTTCTCTATTTGAAGGATATGTTCTTACATTCCCATTTATCCTTTTTGTAATTAATGATTTTGTTTATATGGTTTCTTTTCATGCAATATTGTTTTTTTGCCTTAAACATTGCGTTTTACCCTTTTTTATATAATTAAACTTCATGTGTTTGTATATTATTAGAAATGAAGAACTTATATAGAATTGAATATAATACAAATGTACAAAAGGCTAATCGGAACATCATAAAACTTCAGTCGTCATTTGAAGGGTGCGTCCACATAAAAATTGGAGTGCTGCACTCTTGTGATGGTTAGGAAAGCAGCAAGTGATACCAAAGCCCACAAAGAGAGCATTCCAACGCTTGTTAATAACACTATAGAAAAAATGCTAATGGAATTGATCTTCACCAGGCTGCTTGCACTTGATGCACCAATTAGGTTCAGGTGGATCAAAGGAAATCCTCTTTGCAACTTGTAACTAGTATTGGAACCTTCCTGTAGAAAAATTCACATAAACACCTTAACTTTCTTCAGGACCTTAGCCTTCCAAACTTTGTTGATCAAGTATGAATCCAAGATCAAATCATTATCAAAAAGAGCATTAAACATTTTTGTAACAACTCCTTTATTAATTTATACTCATTGCAGTTGTTTTGCTATCACTATTATTGTTTGGGCCTCGGTGGATTTCTTATCCACTTTCCCCTTTTTGTAATCTTTCCCTGGGAATTTTTTGTTTCTTATAACCCTTTCTTTTGGTTGCCAGAACTCTTCTCATTTGCGAATAGATTTAAGGAGATTTGAACTTGCCTCTTAGTTGCCTTCAAAAACTTCCTAGATGAAGAAAGAAGTGGCAACAATTTCCTGGAGTAACTTCCATGAATTTTTGAATTCTGCCTTTCAGAGAAATTAAGATAAGAAAATCTAGATTACAGCTCAACTGGTTCCATAATATGAAAAGAGTCACCTGCAAAAAAATTTTAACTTGCCTACGAAGTTATTCCATTTTGGTTCTTGAGGTTATTCCATGTCAGTTCTTTCTCTCACGTATCACCTTAGGGCCCTATTTACCTATTTAGTTGTAGGGAATCCAAGATGTTCATAAGAAGGTTATGTGGACCCTGTTTAATTGTAGGGAATCCATGATGGTCATAAGAAGGTTATGTTTGGAAATCTAGGAGTGATGTATTTAATATGAAATTTAAAAGACAGATGTTGGAAGATATGTGAGGTTATTCTTGAGGTTATTCCATGTCAGTCCCCCCTTCCCACCCCCTGCCTTTTTTTTTTTTTTAATGAGAAACAGAACTTTCATTGAGAAACATGAAAGAATACAATATGGCATAAACAAAAAAAAAAAAAAAAACAGCCCACCAAAAGGAGCCAAGCCTAACTATACAAAAAAGGGCTCCAATCTAAAAAAATAAGGCCAAGTGAATAATTACAAAACTCACTGGTAACCGAAACTCAAAGAGAGGCATTAAACCTAGCTACGAACCAAACTTCTTCCCAAGACTCTCAATCCCTTTAAAAATCCTACTATTCCTCTCAAGCCAAATAGCCCACACAACAAAAACAGCTATCCAAAGGAAACTACCCTTTATCTCGAAAAGGAAGATACAAAAGAATCTCATGAAACATATCACGACAATCTCTGCTTTGAGCTAAACAAACCCTAAACAACTCCAAAAAACGATCCCAAATTGCGGAAGTGAACTGGTGTCTCCAAAAAATATAATCAATATCCTCAAGTGCACTTCTACAAAGAATGCACCATTGCAACCCAACAAAAGGACAAATGCCTATGAACCCGATCCATAGTATTAACTCTCTAGAGCAAGAGCTGCCAAGCAAAAATTTGACCTTCTTTGGGATTTTCACCTTCCAAAAGCAACGAAAAAATAGGGGAGCTCGAGGAAGAAGGATTACACAAAAAAGGAACCAAGACTTACAAGAAAACCCCTAAGAGGAATTAAGAACCCAATATTGAATATCTCTCCTCCTCGATCTAATCGAGAAATCCCCTATCAAGGAAAGTAAGGCTGGCCAACACATCAGACGTCTCCTTATCCGACAGGGGATGTTGGAAGCCAAGAACAAGCGACGAAGAATTACCCAAAGACGACAAGATAGAGGCCAAAGAATGAAGCCTCATTGAGGACAAAAAATACAAGCTAGAGAAAACAGTACAAAAGGGTAGTCACGCACACAAATATCCTCCCAAAAATAAGTATTTTTCCCATTTCCAATGGAGCATTTAACAAACTGAGAGAACGGAAAGCCTATGGAGATAGCTTTCCAAAGGTTTCTTGATGTGCCTTTCAACCCACAACTAGTAACCTACTCAAAAGGATGGGGACCGTACTTGCTCACAATAATCCTATGCCACAGGGCATTGGGCTCTAGGAGAAACACCATAACCACTAAGCCAACAGAGCCTCATTGTGCAGTCTCAAGTTGCCTATGCCCAAACCTCACCAGTCCAACAGCTTCGATACCTCCTCCCAATTAACCAAGTGAGAGCCCCCACCTTTGTCAACTCCTTCCCACAAAAAGTCTCATAAATTTCTCTAGGGACTTACTAACAGAAACAAGGATCTTAAATAATGATAGGAAATAAATTGGCATCCTGCTCACAACCCAAGAGGGAAGCCCAACCAAGCAATTTAGAGTGATCACGGTTTAATCCAATGATCGAACATTTAACTCTGTTAATCTTTAAACCAGAAATGGATTTGAAAAAGAATAAATTGCAGTTAAGGTTCAAAAGAAAATAGTGTCATCAACAAACTGAAGATGCGAGAGGTTCAATCCATCCCTCCCGACTCGAAAACCCTCAAGAATTCCTTTCTCCACCCTTCGAAGTCAACCTACTGAGACAACCAACAAGAATAAGAAGGAAGAGGGTCACTCTGTCTAGGACCTTTAGAAGCATAGATCCTACCCCTTGATTTACCATTGATTAGAATAGAGTACGTAACGCTCTTTATACAATTCCAAATCCAAGCTCTCCACTTAAATCCAAAGCCTTTCTTCCAAAGCACCTTGTCTAAGAAATCCCGATCAACAAGATTATAAGCTTTTTCAAAATCAATTTTGAAAATGGCACCTTCTTGCTTACGACTTCTATAGTCCTCAATAGCCTTATTAGCAATAAGAACTTGATCCATAATCGGACTCCCTACAATAAATGCCACTTGGGAATCGAATATCATATTAGGAAGGATATTTCTCAATCTATTAGCCAAAACCTTAGCAATGATCTTATATAAGCCTGTAACAAGACTTATAGGTCGAAAATCCTTAACCTTCCTTACCCAATTCTTCTTAGGAATCAGATAGACATAAGTTTTATTTATTGAAGTATTAATGATCTCTCATAGAATTCCCTAAAGGCCTTCCAAAGATCATCCTTCAAACACTCCCAAGTCTTGAAAAAAAGCCATCGTAAACCATATGGATTGGGTACTTTGTTCCTCTCCAAGCTGAAAACCACTTTTCTAATCTCTTCCAAAGAGAAAGGAGCCTCTAATTTTTCCTTATCCATAGCAAAAATGAGATCCCAATCTAAACCTTCCAGAAAGGGTCTAGGACAGGTTGTAGGGGCATAAAGAGCTGATAAAAAAGACAATCTCATTCTCCACCTCCTTGTCCCCTTGAAAAATATCTCCATTTTTCCCTTCCAAAGATTCAATAGGATTCTTATTCCTTCTCCCATTTGCTACATTATGGAAAAAGGACGAATTACAATTGCCCTTTTTGGCCCATTTCACTTGAGCTTTCTGTCTCTAGCTAGTAGCCTCTCTTTGATCAAGCTCCTCAAACTCTCCATTAGACTTTATGCGCTCCTCCCTCAACTCCTCACTCAAAGTCCCCTCCTCCTTCCGTCCATCCAAAAAATTAATTTTATCCAAAATCTCTCGTTTCTTTAACCTTGTGTCACCAAAAACATTACAATTTCATTCCCTCAAAGCCCCCTTGATGGATGAAAGCTTTCTCATAAAACAAAAACCTTCCCACTTTCCATCGACGTTAATACTCAACCATGAAGAAATGGTAGATTTAAAGGAAGGATGAGATAGCCACATATTCTCGAATCTAAAGGGCAAGTACCCCAATTTATAGGGCCAGAGTCCAGACAAATTGGCTAATGATCGGAAGTGACTCTAGGACTCAAAGATTGTCTAGCACTAATGAACACGTCCATCCACCCTTTCACAATAAGAATCTATCTAGCCTGCAATGTACCACTCTCTTCCTAAGATTAGACCAAGTATACCTACCATTGGCTAGGTAATATTAAGGAGACTACAATCCTCAATGAGGTTATTAAAATTTCTCATGGATTTGGTAACTTTGCCTCCAGTAGAATTTTTTTAGGGGAATCTAGTCACGTTGAAATCTCCCGACAAGCACCAACAAGACCCACACAACCCAATAGGCTCCCCAGCTCTTCCTAAAAGGCTTTCCTCAAGCTAGGTCTAGAAGACCCATACAGACCCGAAACCCACCCTAAGATATCCCCAGGAAACTTGAAGGACATAGAAAGAGAAAAAATCCCTCTCACAACTTCCACCGAATTAACAAGACGCCCATCCCAAAACATTCAGAATACCTCCCGAAGAACCAACAGACTCAAGCCTCACCTACTCCACACCACGACCATTCCACAAACTCTTAGTGAAAGCCCTACTATAACTCGTCGTCTTAGTTTTTGGAAGTATTGCAATATCCAGTTGCTCCTAACTAAGCAACGCTTTAACCAATCTCCTTTTGTCCCTCCCCCTAAAACCTCTAACATTCCAAGAAACAATCTTAATCAGATGACCAAAAAAGCCCAGTTTCCAAAGAAGCCCAATTCCCCTCATAATTAATTGAACATTTTCTCAACTCCCTTGAACCCCTCTTTGGACAGTCCTCACCCCTACAAAGGACCTTTTCCCCTCTTTGGTAAGCTTTTAGGGACCTTCAAGGGAACAATATGAAGCCCTGTATCAGCCAAAAGGGAGCACAAAACCTACCAATTACTAATAAGACGCTCTTGAAATGGAAGACTCCCAGTGGGATCAAGCATGGGCACTGGATGACCAGCAACTGTCCTGGACACAAGAGACTCACCTTCTCCTCCAGAGACACTAACACTCGGCTCACAAGGGTACAAAACAAGAGTTGGATCACTCTCCCTAATCGAAAAAGCCACTATTGTGGAAAAGACACATCCTAACCCCGATAAACTATAATTAATAGCGGATAAAGAGGAAGGAAAAGAGGCCAACATCCCCAACCTAGAATCAATTGAAGGAGACACAACCAAAGGAAGGGAAGACCCAAACATAGGAGAACCCTCAACCCCCTCATTAATCCTCGGGAAAGAAGGGGAAAAAGCCAGAGGAGTGCAAACCTGAGCAATCGAACCTGTGGAGCTAGAACCTTGACCCGAGAAAGGAACATCCAATTGAGAAGGAGATGAAATGGCAGCAGTCCCTACACCTGCAATCTGACCCTTTGTTTTCATCTGCCTCTAGGCTTCAGCCCGTTTTTCATTCTTCTAAGCGTCTCCTCCTTCAAACAAAAGAGCCAAATCCTCTGAGCTTTGATCCCTCAATACCGGAGAGGAAAGGAAGGAATCGTCGTTAGAGAGCTCGCTATAGAAGGATTTATGGTATCCTCTTTTGACCCAAACCCTAAATTTGGGGACCCGTCTACGCGCGCATACTCACCAATCGAAAAAGCAATCTGAAACCAAATTTACCAAAGCCCTAGGTCCAATACTCTTCAGCTCGCTTTGGGTCATAATCAAGCCCCCTTCCTTTGCCCACTACCCGCAGCCACAACTGCATTTGCCGAACAAACATTCCCACTCAACTCAGAATAGATCAAACAACACACTAGCGAACTCAGCCCAACTTTGAACATATCTAACCCAGCCTTAGTCAAGACTGCCTCTGCCTCCTTCGATTTTTTCCTTAAATGGGTTTGAGGGTTACCCAGTTGCAGAGAAATCCCATTTGAATTAATACCTGAATAAGGCAACCCGATCTAAGGAGTGCTGGCCACATTGGTTCTCAACTTTTTCGGTACACTGTCGGCGTTTTGCCCAGGTTTCAAGGATCACTTGCCTGTTCCTTAATCACCAAAACGCTACTCTGGACATCAAATACAAACTCAACCTCCTCCTCCAAATCCCCGATACTAAGTCTTGACCCTAACTCCCCTTCTTTCGTCACTTGGGAAAACAAGGCCAATGTTGGTTCCTTCTTTGGGAAAATAGGGGACTGACGAGGGACCGGGGGGGGGGGGGGAGCACTTGGCAGCAACAACTCCAGGTTCTCCAAAAACACCCTCCAACCAATTCTTTCTCTACCCTCCAGGATGATCACATAAGCTTTCCCCCCCTAGAAGGTTCTAACGCGATGACACAGCACCTTCCCTTGGAGTTGGAGACCAACTGAACCCAAGCTAAAGCTTCCGATAACCGTCAACTCTTAAAGAATGTCTCAGCCTTCGATCGTGAGACATGGAAGGATAAACAATCTCTCACCCACACAACCAAACCCATATGTAACCTCAACAACGACGACTTCTCCCTGTATAATTCTTCTATCGCTTATGTTTGATAAGACTCGCTTATGTGAATTAATTGCAATTATGTCATAATTCTAATCATGCTATTGTATGAAAAAGTTATGTTTATGTCAAAATTTATCCCAAATTAATTCACTTGATGGAAATTTTCAATTTTCTCCTTAATCACATTAGTCGAAATAAATATGATATGAAATTTTTTCTAATTTTAATTTTTATTTGTGTTATCGCACGTACTGATTTTTATGTCATATGTAAGTTGAGACAATTCAAGTAATATATGATATAATTTAGTGCACTACTATTTAGATGACACTCTTCCTTCATCGGCAGAATTGTAAAAAAAGTATGTGATGCATTTTCTGTTTGATTTACCAATTTGGAATGATTGTTGAGTGAATTGAGGTGCAGGATCTGAACCATGTGGCCTTCTTTCTTCATTGGTGGGTGCTGGGCACGAGTGTGATGGTTACAAGGTTCGCATTGTAGGGCATTCCTTAGGAGGAGCTGTTGCAGCATTACTTGGACTAAGAGTATGTTGCAACCTCTATTCCTTTTTTTCCTGTGTGTATGTGTAGTTTTTTTTTTCTTTTTAGGGAGTGATAAAGTCATATTTGGCCTCTAAATTCGGTTGGACTTCTGGTCACCTCCATCTTTTTTTTTGTCCGTTTAGTAAAATTATTGTTTATAAAAAAAAAGTCTCAATTATGGAGTTTGAAGAGAAATTTCCAGCGAAATCATCTAGGAAGGACTGCTAAGTTAACTGATGATTTGGAAAGGGAAACATTGATAAAATAATAGGACATAAGGCTACAACCAATATCCAGTATGGCATAGACATTTAGAAAATATGACAATTGGGGACTGACTAGCACTCAAGTTATCTTTTACTTATTGTTAATTTATTATAAATATTTTTTTTATAATTATGGTAGTTTATGTTCATTAAAAATTCTCATCCAGAGATTGGTTGGATTATATAAGCTGTGCATCCTTCCTTCCCTTCTATTTGGATCATCTTTTCATTCGTTGGCAGATTTTCTATAAACTTTTGGATTTTGGACTGGGTTTCTTTTATTTTAGGTGGGTTTTCTTGGAGCAAGTAGGTCAGCACTGTTCCAACTCGATTTGTTGTGAGCATAAGAGTGAGGAAGCAGTTTTTAGTTTGACTTCCCTGACCTTGACTTTTTAGGAGATCTAGAAAGAGAGGAATTAGTGGATTTTGAACTCAAAAGGTGGATATTGTAGATTTTGAATAAAAATTCTTGTGGCTTCCTGTTGGTCCTCAATCAAAGGACCTTTCTTGTAATATTCCTTAATTTCTAATCTTGATAAGCTGGAAAGCATTTTTTTTGTGATTTTCCTTGACTACCGTAATAACATCTTCACTTATTTTCAGATTTACTGAATGACTCCCATAACAAGTATCAAAACTAATTAATATGATCCTTGATGTGGTGCCTCTGATCTGAATGCACATTTTAATTAATTTATCTACCCACCTCTATTTTTGTTTGTTCCTTATTGTGAAATATCCTTGAATTGATTGACTCTTTGTCCTCAATATCTCTTAAAGTCGCATTTGTTTATTACAATGAATATCCTAACCCTTTGTTATTTTTATTTTCTCTATTTTTCAGTTTTAGATGCACTAAATTCATTTTCAATATCATATGTTTTTGTACAGTTATACCGTCAGTGTCCCAACTTACATGTTTATGCATATGGGCCGCTTCCATGTGTAGATTCCATTATAGCAAGTGCATGTTCAGAATTTGTAACAAGGTAATGTAAATTGCTACAATTGTCAGTGATAGTTTCAAGGTTAAAGGTTATTTATTTTCCACTCTGTATCCCATATGCTTTAAAATTCCTGCAGTATTGTGTTCAACAATGAATTTTCATCTCGACTTTCGATTGGATCCATCATGCGCCTTCGTGCAGCTGCAATTAAAGCGCTGTCACAAGATAGCAAAGATAAGTCGACCCTAATTTTCCACCTTGCTCGTGGATTCCTTTATCTGAGCAACTGTCAAAGAAATGGGATAGAAAGAAAGAATCCTCAATCAGATAAATATTCTAGGCACATTGAGGCTGATGATCAGGGGATCTCCACTAGTTATCATCAGCATGAAACCAGAGGTAGATAGCCTTTTCCTCTGTAGGTACAATCCCAAACATTATTATTAAAATGCCGCTACTTTAAACAATCATTTTCTATGTTATGGAAAGAAGTTAAGAAGTCTAGTAGTGGAGATAAGTATACCGTCAGTTGTAAATTAGTTAGCCTTATGGTGGAAAGGGAATTGATTAAATTAATTAGTTGCGATTTGAGCAGATCAGTTTGAGCGATTAAAAAGAAAAGAAGAAAGAATCTTATTTCCTTCCAAGCCATATATTTGGCAATGTTTTCACCAATTGGTAAGTAGTAACATGTTCCTGATAAAATATCCTGTTGTAACTGCAGCAAACAAGAAAGAGTGCCAAGAATTCTCTCTCTTGGCAAAGAATCAAACCGAAGATAATGTCATCTCAATTGAACGTAATGAGTTCTCCAATTCTGATGATCTGGTATCTCAATTTATGGAAGCTATTGAAAGATCTGAAAATGACTCAACTGAGAGTTTCTCAGAGATGTATCTTCCAGGCCTTTTAATTCATATAGTTCCTCAAGAAAGAAGGTTCACTTTACCTTTCTTGAACAGCTTGAGGGGCCAACCTGGTGCAGATGATTATAAAGCTTATGTAGCAAGCAGAGATGGCTTCAAAGACCTTATTGTGTCACCTTCAATGTTCCTAGATCACCTTCCTTGGAGGTACACTTCTAATATTCTACCTTATTATTCAATTTCCATCACTTCTGGCTATTCATTTACAATTCTATTCTTTTCCTTTTCAGATGTCACGATGCTTTGCAAAAGTTTTGGATGCTCAAACTAACAAAGGTTCACTACATGAAACTCTGACTGTGTAAATAAAAGCACATAATTGAGGTTAATTGTGAACCTTTCTTGGAAATAATAATAATAAAGAAATGATAATTCATTCAACTGTGTTTATTAACCATCGAGGCTTGGCCTAGTGGTCAATGGGGCCAATGAAAAAGTAAAAGGGCTTAGAAGGAATGGATTCAAATCTGGTGGCCATCAACTTAAGATTTAAAATCATATAAGTTTTCTTGACAATCAAATATGGTAGAGTCATGCAGTTGTTCCATGAGATTAGTTGAAGTGCACACGAACTAGCCCAGACACTCACGTTTATCGAAGATAATAATTAAAAAAAAATCAACTGTGTTTATTCTGTTTTCTTTCAAGTTTGAGATAAAAGTTGCTTATTTGATGTTTTTGGTGTGGTCTAATGAACTTTGCCTTGAAAGGAGTCATATAGCAAAAACTATAAATCCTGGTGCTCATCATTATCACCATGTTCCATCCATTATCTTGAATCCAATACAATCTAAATTCCTGGTGCTAATAGCAACTAATGTCATGTACATAGGGTTACTTTTTCCCTTTTTTTTTCTTTTTTTGAAAAAGTCAATTCGGGATAGAGCTGCAAGTCAAAACTATTGATTGATTTGATTAGTTTAAATAATGTGTTGGATAGTTTGGTTTCATGGAAAACTGAAAATCAATTGATGAGTCAGTCCGATTTTGATTGATTTTGTTACTTTCAATTGATTTGATTTGGTTTTAGTCAATTTTGTACATGATTTAATTTAGTTTCAATCAAATTGTGGAAGCAATTTGGTTTTCAAAAAGCCATTTTCATCATCGATCTATTTTCACCACCAAACTTAGACCATTACTCATCCTCGAGTGATGCACCAAAAAGAAAGGAAGTCAAACGAGATTTTCATCTAGGAGCGTAAAGGAAGGTATAAGACCATTTAACCTTTAGGTAGGCTCTCAAAAGTTACTCACAATTAACACAATTGAGCAATAACAAAGTTACTTGGGCATCAAGTGAACTCAATAAGGTGTCTATCCATACCGCTAAAGGCTTACAAATAAAACTCACAAAATAAAGATAAAGTGAAACTCTTTTTTCTCTTTTTTCTTCTTTTTTCTTTCAAGTACATATTTCTTTTCTTCTCTTTTTCTTTCTTTTTCAAACCCAATAAAATAACAAAGAAACAGGGGAGGTATGAACATGACAATTAGCTCTTTTTTTTTTTAAAGGAAAGCCCTACTAGTCATTCATGTGTTCTTTTCAAATGCCGACATTGGTAATGAAGGCACTAGATTTTCTAGCATTATCACCAAACACGACCACTTTCGCATACTCATTTCTCTCATTATCGTTTGAAGCAAAGACAAATTGTGGGTGTCATGAATATCTCTAGTTACAAGGTCCACGAGAGAAAAAGGAGTATCTCTTTTTTTTTTTCACAAGGATATGACCTCCCCCAAACTTGGGATTTACAATAAGAATTTCACTTACCACCCACTTCTTACTATCTTTAAGATTACTTCACAACACCAAACTAATCCACTTTAGCCCCTAAGATCTCTTCAAGATAATAAAGCTTCTTAGAAGCAACAAGATAGGTCAGTAAGGCCTAAGTGTAAAGGGGCACATTCAAGCTACAACTCAACTTGTCATCACCTCATGTGCCTCATGCAAAATAAAATAAGAGAGCTTAAATTATCATAATCAGAAACAGACTAAACCACCAAAAAGCTCAGAAAGACAACTCAAACAACAACAGAATTTTTTTTTTTAATACAAAAAACAACTCAACAAGAGCATAATGAGACCCCAAAACTTAAGGATGTGTTGTGTCCCCACAACTCAAAATGGAATGAACATATCTTAAGCAAGATTAACAATTACTCCAACACTTCAATGACACTCCAACATTCAACCAACCAAAACTTAGATTAGGGAAGCATTAACATGAAAAGAAAGAAAATAGAAAAAGAGAGTTTGGGTAAGAAAGCTCCCCTGCTTTTTTTTTTTTCCTACTACAATGCTACAACAACGCTACCTCAACTGGAGCAGATTTTCACACAAAAAATAGAGAACTCCCAGCTCTTGAACTTTAATTCCTCCTTCCTTTCATGAGTTTTCTCTCTTTTCACCTAAAAAAAAGGAATATCAAGAAGTTCTGAACCAATGAGTTCTCAAACACTATAGACGGCCAAGGAAACATAAATATAAAGAGAAAGGAGAGAAAAAAAAAGACAGCAAACAAAAACAAAAAAAAAAAATGGAAATACGTAGCTTGACTTAAAAGGTCTTGGATCTTTCAAAGAACTTGAAGCTCTTTTCCATTCTTTTTCCCTTTCTTTCTTCCTCTTTCTCTTCCTTTTCTTTTTTTGAAACCTTGTTCTTTGATTTGTAGTTTCGTGAAATTACTTGATTCAAAGTGCAACTCCCACATATGCTTCTCCCTCTCATTTGGGTGACACATTTTTTTTTTACAAACTCATCCATTCAACTTGCTTCCCTTATATGAATGGTTGTTTATTCTCCATCCATGAGATTAGAGCATCCTCAACTTCACTTTCTTCAATAGAATGTTCAAATGAGGCCACATTCATTAAGCTCTTGTTGCAACTTTTTTTCACTTTTTCATTCCCAGTTGAGTTCGAAACACTAATGGTAGAATATTCTTCCACATCATTAAAGGAATGCATAGTATTGAACACAGTAAAGGTTACCTGTAATCATTCATTCTCATGGCAAGCTCGCATTTCTCTACATCAATCAATGTTCTTCTACTAGCTAGAAATGGCCTACCTAAAATTATAGGAACCTCTTTATCAGCTTCATAGTCCAATATAATGAAATTTACAGGAAAAATGAATTTATCCACTTGAACAAGAACATCTTCAATTTTTCCTTCAGGATGAGTATAAGATCGATCTGCTAGTTGAAGCGTAACCGTAGTGGGTCTTACTTCTCCAATTCCAAGCTTTTTGAAAACTAACATAGAAATTAAGTTGAAGTTTGCTCCCAAATCACATAAAGCGCTTCCGCAATAGGTTTTACCAATTGAGAATGGGATAGTAAAAATACCAGGATCCTTCAATTTAGGAGGAAGCTTGCTTTGTATGATTGAGCTACAATCTTTTGTAAGGGCCACTATTTTAAATTCCCCAAGCCTTCACTTTTTGGTTAAAATTTCTTTCATGAACTTGGCATAATTGGCATAATAGCATAATATTAATGTGTAATCCCTTCAAAATGTCTAAAAACTTATCAAACTGCTTGTCTTGCTCTTTCTTTTGAACTCGTTGGGGAAATGGTGGCGAGGTTTCACTAACTGCTGTTCTCCTATACTTTGTTGTGATAATGCTATAGCAATCCTCTGTTCTGCTGTTTTCTGCATTATCTCTTCTTCACTGCCGCCCTTTGCATTCTGTTCACTTTTCTCATTTTTCTTTTCGATTGAAGTGGGCTCTTTCAGCTTAATGGTGCTGCTCTCAAGTGGATCCAACTCTTTCCCACTTCTAAGTTGGATTGCTTTGCAAGTATCTTTGCCTTTACTCTTTGGATTCTCAGTATTACTTGGCAAAGTCCCATGAGCTCTATTATTTAAGGCTGAAGCTAATTGACCCACTTGATTTTCCAAGTTCCTTAGAGCAGCGGCTTGGCTTTGAACTATGGCATCAGTCTTAGCCATGTGCTCTTTCAACAAACTCTCAATAGAAGACATAGGGTCTTGAATATGTACCTTTTGTGTTTGTGAAGTTTGTGGAGGATGGAATGGTTGCTGAAAACCCGGTGGTGCAGGCGTTCTTATCAGTAAGTGCGTAGGACTAGCTCCTGGATTATTCCACGAAAAGTTTGTATGTTACCTCCAACCTAGATTATTTGTGTTTGAGTAAGGATTGAAGTCATTGTTACAGCCAACATAGTGCACAGAGGTGGGATTTGATGGACAAACATCAAAAGCATGCCCCCTCCACAATAAACACATGATATGATTGCTAGTTGTCTATATGAACTCCTTACCCTCAAGCTCAAAGATTTCAACGTGTTGTTCAAAGTGGCTATCTGTGCTGCTAGATTTATTGCATCAATGTCCTGCATTCCAGCCACCTTTCTTGCTTAGTTTAGACGAGATGTGGGCCACTGATAATTGTTCTTTGCAATTTTATCCACAATATCATATGCCTTATTATACGTCTTTGAAAGTAATGCCCCATCAGTTGACGTGTCCACAACCATTCTTGTTTGAGCTTTCTAACCGTTATAAAAGATCTTCATTTGAATACCATAAGGAATGTCGTGGTTGGGACATTTGCGCAATAGCGCCTTGAATCTCTCCCATGTTTCATACAAGGATGCATCCTCCAATTGTTGAAATGATGTGATCTCGCTTCTAAACTTGGCTATCTTCGTAGGTGGGAAGTACTTCGTCAAGAATT

mRNA sequence

ATGCGCGAAAAGTGGAATCATGAAGAGATAGTTGGACAAATGGCGCAAAGAAGGGACCAAGAAACTTCTACCTTCCATGAACCATTGATTCCCTCCGTTTGCTCTTCAAACCACTCGAACCATCAAATTCGATCACCCATCCCCACATTTCGATCACCATGGTGTCCCATTCGAAGTAGAACGGAGGATATGGGGGTGTCGAAGTTGGAGAATATTCGGTGCTCAACCATCGTGTTGGCCATTTCTAATGCCCTCGTCGCCCTTCTCGGCGCCTTTCTTCTCATCGTTGTTTATCCTGCTTGTGAACGCCAATATGTCCTCCCATTTTTGGCCGTCTCTATGGCTTCTTGCATTAAAATCGTCACCATGGTTCAGTGTGGCATTGCCCAGGAGGCTACAGCCAGAACCATTCTCGAATCTCCTGGTGATACTGCTGCTGTTGTCAACACCATTATGCGCCACGAGAGACGGTTGAGGTATAAAAGATGGCTTTGGTGGACTCGTTTTGCTCTGGTCATTACAGTGCTGCAATTTGTTGGAGCATTTTACTTTATCTTCCATATGGCTAGCTACATTGCTCATGATGACACTTCAAGTGGTTGTGCTTTAGGGGTGGCGTCAAATGATAGATGGTGGAGACGAAAGCTACTGGTTTTATTTACAATCTTGGTGTGCTTTGTTGCACTGGTTCAATGTTTTACTGGTATGGATGTTCTAAGGTGGAGATCATTTTATGAAACTCAAGATCATGCGTGGAAAGCACATTACAGTGAGATATTTGATCATGGAATTCGTGAGGCATTATGTTGTTTGGGACGTGCTAAATACTTGAGCGTCATGGAAGAAGATGAGATCTTCTCAGTGGCGCAATTATTGGGTGATCTTGTTGCTTATCGTTCATCTGGCACTGGGCATTTGGAATTTTTGGCAGGCAGCCCATACTCCATGGTGATAACTGGTGGCGAGGCCATGCTGCAGCCTTTCTTAAATATGTCAAGTTATCACCTGAAGTACTTCGAAGAGGACGTGTTAACCAGTAACGATCATGTTCACCCCAATGTGAAGCAGAAGATTAGATCATCCTTCCCACACTATGCACATTCTGGGATTGTTGAGGCTGCCAGGGATTTATATATGCAAATTGAAGGAAATTCTAGAGATGATGATGGATCTGAACCATGTGGCCTTCTTTCTTCATTGGTGGGTGCTGGGCACGAGTGTGATGGTTACAAGGTTCGCATTGTAGGGCATTCCTTAGGAGGAGCTGTTGCAGCATTACTTGGACTAAGATTATACCGTCAGTGTCCCAACTTACATGTTTATGCATATGGGCCGCTTCCATGTGTAGATTCCATTATAGCAAGTGCATGTTCAGAATTTGTAACAAGTATTGTGTTCAACAATGAATTTTCATCTCGACTTTCGATTGGATCCATCATGCGCCTTCGTGCAGCTGCAATTAAAGCGCTGTCACAAGATAGCAAAGATAAGTCGACCCTAATTTTCCACCTTGCTCGTGGATTCCTTTATCTGAGCAACTGTCAAAGAAATGGGATAGAAAGAAAGAATCCTCAATCAGATAAATATTCTAGGCACATTGAGGCTGATGATCAGGGGATCTCCACTAGTTATCATCAGCATGAAACCAGAGCAAACAAGAAAGAGTGCCAAGAATTCTCTCTCTTGGCAAAGAATCAAACCGAAGATAATGTCATCTCAATTGAACGTAATGAGTTCTCCAATTCTGATGATCTGGTATCTCAATTTATGGAAGCTATTGAAAGATCTGAAAATGACTCAACTGAGAGTTTCTCAGAGATGTATCTTCCAGGCCTTTTAATTCATATAGTTCCTCAAGAAAGAAGGTTCACTTTACCTTTCTTGAACAGCTTGAGGGGCCAACCTGGTGCAGATGATTATAAAGCTTATGTAGCAAGCAGAGATGGCTTCAAAGACCTTATTGTGTCACCTTCAATGTTCCTAGATCACCTTCCTTGGAGATGTCACGATGCTTTGCAAAAGTTTTGGATGCTCAAACTAACAAAGGTGGGAAGTACTTCGTCAAGAATT

Coding sequence (CDS)

ATGCGCGAAAAGTGGAATCATGAAGAGATAGTTGGACAAATGGCGCAAAGAAGGGACCAAGAAACTTCTACCTTCCATGAACCATTGATTCCCTCCGTTTGCTCTTCAAACCACTCGAACCATCAAATTCGATCACCCATCCCCACATTTCGATCACCATGGTGTCCCATTCGAAGTAGAACGGAGGATATGGGGGTGTCGAAGTTGGAGAATATTCGGTGCTCAACCATCGTGTTGGCCATTTCTAATGCCCTCGTCGCCCTTCTCGGCGCCTTTCTTCTCATCGTTGTTTATCCTGCTTGTGAACGCCAATATGTCCTCCCATTTTTGGCCGTCTCTATGGCTTCTTGCATTAAAATCGTCACCATGGTTCAGTGTGGCATTGCCCAGGAGGCTACAGCCAGAACCATTCTCGAATCTCCTGGTGATACTGCTGCTGTTGTCAACACCATTATGCGCCACGAGAGACGGTTGAGGTATAAAAGATGGCTTTGGTGGACTCGTTTTGCTCTGGTCATTACAGTGCTGCAATTTGTTGGAGCATTTTACTTTATCTTCCATATGGCTAGCTACATTGCTCATGATGACACTTCAAGTGGTTGTGCTTTAGGGGTGGCGTCAAATGATAGATGGTGGAGACGAAAGCTACTGGTTTTATTTACAATCTTGGTGTGCTTTGTTGCACTGGTTCAATGTTTTACTGGTATGGATGTTCTAAGGTGGAGATCATTTTATGAAACTCAAGATCATGCGTGGAAAGCACATTACAGTGAGATATTTGATCATGGAATTCGTGAGGCATTATGTTGTTTGGGACGTGCTAAATACTTGAGCGTCATGGAAGAAGATGAGATCTTCTCAGTGGCGCAATTATTGGGTGATCTTGTTGCTTATCGTTCATCTGGCACTGGGCATTTGGAATTTTTGGCAGGCAGCCCATACTCCATGGTGATAACTGGTGGCGAGGCCATGCTGCAGCCTTTCTTAAATATGTCAAGTTATCACCTGAAGTACTTCGAAGAGGACGTGTTAACCAGTAACGATCATGTTCACCCCAATGTGAAGCAGAAGATTAGATCATCCTTCCCACACTATGCACATTCTGGGATTGTTGAGGCTGCCAGGGATTTATATATGCAAATTGAAGGAAATTCTAGAGATGATGATGGATCTGAACCATGTGGCCTTCTTTCTTCATTGGTGGGTGCTGGGCACGAGTGTGATGGTTACAAGGTTCGCATTGTAGGGCATTCCTTAGGAGGAGCTGTTGCAGCATTACTTGGACTAAGATTATACCGTCAGTGTCCCAACTTACATGTTTATGCATATGGGCCGCTTCCATGTGTAGATTCCATTATAGCAAGTGCATGTTCAGAATTTGTAACAAGTATTGTGTTCAACAATGAATTTTCATCTCGACTTTCGATTGGATCCATCATGCGCCTTCGTGCAGCTGCAATTAAAGCGCTGTCACAAGATAGCAAAGATAAGTCGACCCTAATTTTCCACCTTGCTCGTGGATTCCTTTATCTGAGCAACTGTCAAAGAAATGGGATAGAAAGAAAGAATCCTCAATCAGATAAATATTCTAGGCACATTGAGGCTGATGATCAGGGGATCTCCACTAGTTATCATCAGCATGAAACCAGAGCAAACAAGAAAGAGTGCCAAGAATTCTCTCTCTTGGCAAAGAATCAAACCGAAGATAATGTCATCTCAATTGAACGTAATGAGTTCTCCAATTCTGATGATCTGGTATCTCAATTTATGGAAGCTATTGAAAGATCTGAAAATGACTCAACTGAGAGTTTCTCAGAGATGTATCTTCCAGGCCTTTTAATTCATATAGTTCCTCAAGAAAGAAGGTTCACTTTACCTTTCTTGAACAGCTTGAGGGGCCAACCTGGTGCAGATGATTATAAAGCTTATGTAGCAAGCAGAGATGGCTTCAAAGACCTTATTGTGTCACCTTCAATGTTCCTAGATCACCTTCCTTGGAGATGTCACGATGCTTTGCAAAAGTTTTGGATGCTCAAACTAACAAAGGTGGGAAGTACTTCGTCAAGAATT

Protein sequence

MREKWNHEEIVGQMAQRRDQETSTFHEPLIPSVCSSNHSNHQIRSPIPTFRSPWCPIRSRTEDMGVSKLENIRCSTIVLAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIVTMVQCGIAQEATARTILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAFYFIFHMASYIAHDDTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHAWKAHYSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAGSPYSMVITGGEAMLQPFLNMSSYHLKYFEEDVLTSNDHVHPNVKQKIRSSFPHYAHSGIVEAARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLGLRLYRQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIKALSQDSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSYHQHETRANKKECQEFSLLAKNQTEDNVISIERNEFSNSDDLVSQFMEAIERSENDSTESFSEMYLPGLLIHIVPQERRFTLPFLNSLRGQPGADDYKAYVASRDGFKDLIVSPSMFLDHLPWRCHDALQKFWMLKLTKVGSTSSRI
Homology
BLAST of Sgr021835 vs. NCBI nr
Match: XP_022150369.1 (sn1-specific diacylglycerol lipase beta isoform X1 [Momordica charantia])

HSP 1 Score: 978.8 bits (2529), Expect = 2.4e-281
Identity = 534/787 (67.85%), Postives = 589/787 (74.84%), Query Frame = 0

Query: 14  MAQRRDQETSTFHEPLI-PSVCSSNHSNHQIRSPIPTFRSPWCPIRSRTEDMGV-SKLEN 73
           M +R  +E    HEPLI P  CS   S HQIRSPIP+ RSPWCPIRSRTE MG  SKL+N
Sbjct: 1   MERRIGEEICGLHEPLILPISCSI--SEHQIRSPIPSIRSPWCPIRSRTEAMGASSKLDN 60

Query: 74  IRCSTIVLAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIVTMVQCGIAQE 133
           IRCSTIVLAISNA V +LGA+L+IVV+PAC R+YVLPFLAVS+ASCI+IV MVQ GIAQE
Sbjct: 61  IRCSTIVLAISNAAVIILGAYLVIVVFPACGRRYVLPFLAVSVASCIRIVAMVQSGIAQE 120

Query: 134 ATARTILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAFYFIFHMASY 193
           ATARTILESPGD+ AVVNT++RHERRL YKRWLWWTRFALVIT+LQ VGA YFI +MA Y
Sbjct: 121 ATARTILESPGDSPAVVNTVLRHERRLSYKRWLWWTRFALVITMLQCVGAVYFIVNMAYY 180

Query: 194 IAHDDTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHA 253
           IAH +T  GCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHA
Sbjct: 181 IAHAETPGGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHA 240

Query: 254 WKAHYSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAG 313
           WKAHYSEIFD+GIREALCCLGR+KYLSVMEEDE+ SVAQLLGDLVAYRSSGTGHLEFLAG
Sbjct: 241 WKAHYSEIFDYGIREALCCLGRSKYLSVMEEDEVSSVAQLLGDLVAYRSSGTGHLEFLAG 300

Query: 314 -------------------SPYSMV-------------ITG------------------G 373
                              +P   +              TG                   
Sbjct: 301 LALLQRHGQSTQSSEELMEAPIDKIRDAAVLHKFAEAAYTGPLLDFGRNPCSFPCVWLYR 360

Query: 374 EAMLQPF------------------------------------LNMSSYHLKYF------ 433
           + ML P+                                    +N +     YF      
Sbjct: 361 QGMLTPWTRNKRPILHGDNWWRGHAAAFLKYVKLSPEVLRKGRVNQAKCEAAYFVLVLHD 420

Query: 434 -----------------------------EEDV--LTSNDHVHPNVKQKIRSSFPHYAHS 493
                                        EED+  L ++DHV PNVKQKI SSFPHYAHS
Sbjct: 421 VRCVVIAVRGTETPEDLITDGLCRECALSEEDLDGLMNSDHVQPNVKQKIISSFPHYAHS 480

Query: 494 GIVEAARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLG 553
           GIVEAARDLYMQIEGN +DD+GSEPCGLLSSL+G G ECDGY+VRIVGHSLGGA+AALLG
Sbjct: 481 GIVEAARDLYMQIEGNRKDDNGSEPCGLLSSLLGPGCECDGYEVRIVGHSLGGAIAALLG 540

Query: 554 LRLYRQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIK 613
           LRLYRQ PNLHVYAYGPLPCVDSIIASACSEFVTSIVF+NEFSSRLS GS+MRLRAAAIK
Sbjct: 541 LRLYRQFPNLHVYAYGPLPCVDSIIASACSEFVTSIVFSNEFSSRLSGGSVMRLRAAAIK 600

Query: 614 ALSQDSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSYHQHE 673
           ALSQDS DKST IF LAR FLYL+N QRNG E KN QSDKY+RHIEADD GIS+SY Q+E
Sbjct: 601 ALSQDSNDKSTQIFELARRFLYLNNQQRNGSEGKNSQSDKYARHIEADDLGISSSYQQNE 660

Query: 674 TRANKKECQEFSLLAKNQTEDNVISIERNEFSNSDDLVSQFMEAIERSENDS-TESFSEM 675
           TR NKKE QEFSLLA+NQTED++I++ERN+FSNSDDLVSQFMEAIERSEND  TESFS++
Sbjct: 661 TRENKKEGQEFSLLAENQTEDDIITVERNKFSNSDDLVSQFMEAIERSENDKPTESFSDV 720

BLAST of Sgr021835 vs. NCBI nr
Match: KAG6575339.1 (Sn1-specific diacylglycerol lipase beta, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 958.7 bits (2477), Expect = 2.6e-275
Identity = 516/814 (63.39%), Postives = 589/814 (72.36%), Query Frame = 0

Query: 2   REKWNHEEIVGQMAQRRDQETSTFHEPLIPSVCSSNHSNHQIRSPIPTFRSPWCPIRSRT 61
           REKW+ E++VG+  +R D   S  HEPLIPS   SN S++QI SP+PTF SPWCPIRSRT
Sbjct: 9   REKWDREDVVGRTERRMDHGNSDIHEPLIPSDSPSN-SSYQIPSPVPTFPSPWCPIRSRT 68

Query: 62  EDMGVSKLENIRCSTIVLAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIV 121
           E MG SKLENIR STIVL ISNA VALLG FL++VVYPACERQY+LPF+ VS+ SCI+IV
Sbjct: 69  EAMGASKLENIRFSTIVLGISNAAVALLGVFLVVVVYPACERQYILPFVGVSVVSCIRIV 128

Query: 122 TMVQCGIAQEATARTILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGA 181
            MVQ GIAQEATARTILESPGDTAAVV+T+MR ERRLRYK+WLWWTRFALVI +LQ  GA
Sbjct: 129 AMVQSGIAQEATARTILESPGDTAAVVDTVMRRERRLRYKKWLWWTRFALVIVMLQLGGA 188

Query: 182 FYFIFHMASYIAHDDTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRW 241
            Y I+HMA+YIAHD+TS+GC L VASN RWW+RKLLVLFTILVCFVALVQCF GMDVLRW
Sbjct: 189 VYLIYHMANYIAHDETSTGCTLVVASNQRWWKRKLLVLFTILVCFVALVQCFAGMDVLRW 248

Query: 242 RSFYETQDHAWKAHYSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSS 301
           RSFY TQDHAWKAHYSE+FDHGIREALCC+GR+KYLSVMEEDE+FSVAQLLGDLVAYRSS
Sbjct: 249 RSFYATQDHAWKAHYSEMFDHGIREALCCMGRSKYLSVMEEDEVFSVAQLLGDLVAYRSS 308

Query: 302 GTGHLEFLAG-------------------------------------------------- 361
           GTGH EFLAG                                                  
Sbjct: 309 GTGHFEFLAGLALLQRHGQQVQISEELMEAPIDKIEEAAVLHKFAEAAYTGPLLDFGRNP 368

Query: 362 --SPYSMVITGG----------------------------------EAMLQPFLNMSSYH 421
              P + V   G                                  E + +  +N S   
Sbjct: 369 LLFPCAWVYRQGIFTPWTRNKRPVLHGDNWWRGHAAAFLKYVKLSPEVLRRGRVNQSKCE 428

Query: 422 LKYF-------------------EEDVLTS-----------------NDHVHPNVKQKIR 481
             YF                    ED++T                  N  + PNVKQ+I 
Sbjct: 429 AAYFVLVLHDVKCVVIAVRGTETPEDLITDGLCRECALSEEDLDGLINGDIQPNVKQRIM 488

Query: 482 SSFPHYAHSGIVEAARDLYMQIEGNSRDDD--GSEPCGLLSSLVGAGHECDGYKVRIVGH 541
           SSFPH+AHSGIVEAARDLYMQIEGN RD+D  GSEPCGLLSSL+G G ECDGY+VRIVGH
Sbjct: 489 SSFPHHAHSGIVEAARDLYMQIEGNCRDNDGAGSEPCGLLSSLLGPGCECDGYQVRIVGH 548

Query: 542 SLGGAVAALLGLRLYRQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIG 601
           SLGGA+AALLGLRLY +CP+LHVYAYGPLPCVDSIIASACS+FVTSIVFNNEFSSRLS+G
Sbjct: 549 SLGGAIAALLGLRLYSRCPSLHVYAYGPLPCVDSIIASACSDFVTSIVFNNEFSSRLSVG 608

Query: 602 SIMRLRAAAIKALSQDSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADD 661
           SI+RLRAAAIKALS+DSKDKST +F LAR FLY+S  Q++G ERKN QS+KY R I+ADD
Sbjct: 609 SILRLRAAAIKALSKDSKDKSTPVFELARRFLYVS--QKDGTERKNSQSEKYPREIQADD 668

Query: 662 QGISTSYHQHETRANKKECQEFSLLAKNQTEDNVISIERNEFSNSDDLVSQFMEAIERSE 690
           Q ISTSY Q+E+R +K ECQEFSLLA+NQ ++N  ++ER+EFSNSD LVSQF+EA+E S+
Sbjct: 669 QRISTSYQQNESRVDKNECQEFSLLAENQAKENDTTVERDEFSNSDALVSQFIEAVEGSD 728

BLAST of Sgr021835 vs. NCBI nr
Match: XP_038884281.1 (diacylglycerol lipase-beta isoform X4 [Benincasa hispida])

HSP 1 Score: 956.8 bits (2472), Expect = 1.0e-274
Identity = 514/783 (65.64%), Postives = 573/783 (73.18%), Query Frame = 0

Query: 16  QRRDQETSTFHEPLIPSVCSSNHSNHQIRSPIPTFRSPWCPIRSRTEDMGVSKLENIRCS 75
           +R DQE ST H+PLIPS C  N +NHQIR PIP+FRSPW P RSRTE MGVS+LENIRCS
Sbjct: 2   ERTDQENSTLHQPLIPSNCPLN-ANHQIRLPIPSFRSPWSPSRSRTEAMGVSRLENIRCS 61

Query: 76  TIVLAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIVTMVQCGIAQEATAR 135
           TIVL ISNA +ALLG FL++VVYP+CERQYVLPFL VS+ SCI+IVTMVQ GIAQEATAR
Sbjct: 62  TIVLGISNAAIALLGGFLIVVVYPSCERQYVLPFLVVSLVSCIRIVTMVQSGIAQEATAR 121

Query: 136 TILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAFYFIFHMASYIAHD 195
           TILESPGD AAVV+T+MR ERRL YKRWLWWTRFAL++ +LQF GA Y IFHM +YIAHD
Sbjct: 122 TILESPGDAAAVVDTVMRRERRLVYKRWLWWTRFALILVLLQFAGAVYLIFHMTNYIAHD 181

Query: 196 DTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHAWKAH 255
           +TSSGCALGVASNDRWW+RKLLVLFT LVCFVALVQCFTGMDVLRWRSFY TQDHAWKAH
Sbjct: 182 ETSSGCALGVASNDRWWKRKLLVLFTTLVCFVALVQCFTGMDVLRWRSFYSTQDHAWKAH 241

Query: 256 YSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAGSPY- 315
           YSEIFDHGIREALCCLGR+KYLS+ EEDE+FSVAQLLGDLVAYRS+GTGHLEFLAG    
Sbjct: 242 YSEIFDHGIREALCCLGRSKYLSITEEDEVFSVAQLLGDLVAYRSTGTGHLEFLAGLALL 301

Query: 316 ----SMVITGGEAMLQPF------------------------------------------ 375
                +V T  E M  P                                           
Sbjct: 302 QRHGQVVHTSEELMEAPIDKIEEAAVLHKFAEAAYTGPLLDFGRNPLSFPCAWAYRQGIL 361

Query: 376 ---------------------------------------LNMSSYHLKYF---------- 435
                                                  +N +     YF          
Sbjct: 362 TPWTRNKRPVLHGDNWWRGHAAAFLKYVNLPPEVLRRGRVNQAKCEAAYFVLVLHDVKCV 421

Query: 436 -------------------------EEDV--LTSNDHVHPNVKQKIRSSFPHYAHSGIVE 495
                                    EED+  L + DH+ P+VKQ+I SSFPH+AHSGIVE
Sbjct: 422 VIAVRGTETPEDLITDGLCRECALSEEDLDGLINCDHIQPSVKQRIISSFPHHAHSGIVE 481

Query: 496 AARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLGLRLY 555
           AARDLYMQIEG+  D  GSEPCGLLSSL+G G ECDGY+VRIVGHSLGGA+AALLGLRLY
Sbjct: 482 AARDLYMQIEGDC-DGAGSEPCGLLSSLLGPGCECDGYQVRIVGHSLGGAIAALLGLRLY 541

Query: 556 RQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIKALSQ 615
            +CP+L VYAYGPLPCVDSIIA+ACSEFVTSIVFNNEFSSRLS+GSIMRLR+AAIK LSQ
Sbjct: 542 GRCPSLQVYAYGPLPCVDSIIANACSEFVTSIVFNNEFSSRLSVGSIMRLRSAAIKTLSQ 601

Query: 616 DSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSYHQHETRAN 675
           DSKDKST IF LAR FLYLSN QR+G ER N QS+KY   IEADDQGISTS+ Q+ETRA+
Sbjct: 602 DSKDKSTPIFQLARRFLYLSNYQRDGTERTNSQSEKYPTQIEADDQGISTSFQQNETRAD 661

BLAST of Sgr021835 vs. NCBI nr
Match: XP_008445624.1 (PREDICTED: sn1-specific diacylglycerol lipase beta isoform X4 [Cucumis melo])

HSP 1 Score: 951.0 bits (2457), Expect = 5.5e-273
Identity = 511/783 (65.26%), Postives = 573/783 (73.18%), Query Frame = 0

Query: 16  QRRDQETSTFHEPLIPSVCSSNHSNHQIRSPIPTFRSPWCPIRSRTEDMGVSKLENIRCS 75
           +RRDQE ST H+PLIPS    N SNHQIR PIPTF+SP CPIR+RTE MG SK++NIRCS
Sbjct: 2   ERRDQENSTLHQPLIPSDFPLN-SNHQIRLPIPTFQSPLCPIRNRTEAMGTSKMDNIRCS 61

Query: 76  TIVLAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIVTMVQCGIAQEATAR 135
           T VL ISNA +ALLG F++ V+YP+CER+Y LPFLAVS+ SCI+IVTMVQ GIAQEATAR
Sbjct: 62  TFVLGISNAAIALLGGFIIFVLYPSCERRYTLPFLAVSLVSCIRIVTMVQSGIAQEATAR 121

Query: 136 TILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAFYFIFHMASYIAHD 195
           TILESPGDTAAV++T+MR ERRLRYKRWLWWTRFAL++ ++QF GA Y IFHM ++I HD
Sbjct: 122 TILESPGDTAAVIDTVMRRERRLRYKRWLWWTRFALILVMMQFAGAVYLIFHMTNHIGHD 181

Query: 196 DTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHAWKAH 255
           +TSSGCALGVAS+DRW +RKLLVLFTILVCFVALVQCFTGMDVLRWRSFY TQDHAWKAH
Sbjct: 182 ETSSGCALGVASHDRWLKRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYSTQDHAWKAH 241

Query: 256 YSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAGSPY- 315
           YSEIFDHGIRE LCCLGR+KYLSVMEEDE+FSVAQLLGDLV YRS+GTGHLEFLAG    
Sbjct: 242 YSEIFDHGIREVLCCLGRSKYLSVMEEDEVFSVAQLLGDLVTYRSTGTGHLEFLAGLALL 301

Query: 316 ----SMVITGGEAMLQPF------------------------------------------ 375
                +V T  E M  P                                           
Sbjct: 302 QRHGQLVHTSEELMEAPIDKIEEAAVLHKFAEAAYTGPLLDFGRNPLSFPCAWVYRQGIL 361

Query: 376 ---------------------------------------LNMSSYHLKYF---------- 435
                                                  +N +     YF          
Sbjct: 362 TPWTRNKRPVLHGDNWWRGHAAAFLKYVKLPPEVLRRGRVNQAKCEAAYFVLVLHDVKCV 421

Query: 436 -------------------------EEDV--LTSNDHVHPNVKQKIRSSFPHYAHSGIVE 495
                                    EED+  L + DH+  +VKQ+I SS PH+AHSGIVE
Sbjct: 422 VIAVRGTETPEDLITDGLCREYTLTEEDLDGLINCDHIQLSVKQRIISSSPHHAHSGIVE 481

Query: 496 AARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLGLRLY 555
           AAR+LYMQIEGN +D DGSEPCGLLSSL+G G ECDGY+VRIVGHSLGGA+AALLGLRLY
Sbjct: 482 AARELYMQIEGNCKDHDGSEPCGLLSSLLGPGCECDGYQVRIVGHSLGGAIAALLGLRLY 541

Query: 556 RQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIKALSQ 615
            +CP+LHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLS+GSIMRLRAAAIKALSQ
Sbjct: 542 GRCPSLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSVGSIMRLRAAAIKALSQ 601

Query: 616 DSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSYHQHETRAN 675
           DSKDKST IF LAR FLYLSN QR+  ER N QS+KY R IEADDQGIS+SY Q+ETRA 
Sbjct: 602 DSKDKSTPIFQLARKFLYLSNYQRDVKERMNSQSEKYPRQIEADDQGISSSYQQNETRAI 661

BLAST of Sgr021835 vs. NCBI nr
Match: XP_016900150.1 (PREDICTED: sn1-specific diacylglycerol lipase beta isoform X3 [Cucumis melo])

HSP 1 Score: 948.7 bits (2451), Expect = 2.7e-272
Identity = 511/784 (65.18%), Postives = 573/784 (73.09%), Query Frame = 0

Query: 16  QRRDQETSTFHEPLIPSVCSSNHSNHQIRSPIPTFRSPWCPIRSRTEDMGVSKLENIRCS 75
           +RRDQE ST H+PLIPS    N SNHQIR PIPTF+SP CPIR+RTE MG SK++NIRCS
Sbjct: 2   ERRDQENSTLHQPLIPSDFPLN-SNHQIRLPIPTFQSPLCPIRNRTEAMGTSKMDNIRCS 61

Query: 76  TIVLAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIVTMVQCGIAQEATAR 135
           T VL ISNA +ALLG F++ V+YP+CER+Y LPFLAVS+ SCI+IVTMVQ GIAQEATAR
Sbjct: 62  TFVLGISNAAIALLGGFIIFVLYPSCERRYTLPFLAVSLVSCIRIVTMVQSGIAQEATAR 121

Query: 136 TILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAFYFIFHMASYIAHD 195
           TILESPGDTAAV++T+MR ERRLRYKRWLWWTRFAL++ ++QF GA Y IFHM ++I HD
Sbjct: 122 TILESPGDTAAVIDTVMRRERRLRYKRWLWWTRFALILVMMQFAGAVYLIFHMTNHIGHD 181

Query: 196 DTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHAWKAH 255
           +TSSGCALGVAS+DRW +RKLLVLFTILVCFVALVQCFTGMDVLRWRSFY TQDHAWKAH
Sbjct: 182 ETSSGCALGVASHDRWLKRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYSTQDHAWKAH 241

Query: 256 YSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAGSPY- 315
           YSEIFDHGIRE LCCLGR+KYLSVMEEDE+FSVAQLLGDLV YRS+GTGHLEFLAG    
Sbjct: 242 YSEIFDHGIREVLCCLGRSKYLSVMEEDEVFSVAQLLGDLVTYRSTGTGHLEFLAGLALL 301

Query: 316 ----SMVITGGEAMLQPF------------------------------------------ 375
                +V T  E M  P                                           
Sbjct: 302 QRHGQLVHTSEELMEAPIDKIEEAAVLHKFAEAAYTGPLLDFGRNPLSFPCAWVYRQGIL 361

Query: 376 ---------------------------------------LNMSSYHLKYF---------- 435
                                                  +N +     YF          
Sbjct: 362 TPWTRNKRPVLHGDNWWRGHAAAFLKYVKLPPEVLRRGRVNQAKCEAAYFVLVLHDVKCV 421

Query: 436 ---------EEDVLTS-----------------NDHVHPNVKQKIRSSFPHYAHSGIVEA 495
                     ED++T                  NDH+  +VKQ+I SS PH+AHSGIVEA
Sbjct: 422 VIAVRGTETPEDLITDGLCREYTLTEEDLDGLINDHIQLSVKQRIISSSPHHAHSGIVEA 481

Query: 496 ARDLYMQIEGNSRDDD--GSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLGLRL 555
           AR+LYMQIEGN +D D  GSEPCGLLSSL+G G ECDGY+VRIVGHSLGGA+AALLGLRL
Sbjct: 482 ARELYMQIEGNCKDHDGAGSEPCGLLSSLLGPGCECDGYQVRIVGHSLGGAIAALLGLRL 541

Query: 556 YRQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIKALS 615
           Y +CP+LHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLS+GSIMRLRAAAIKALS
Sbjct: 542 YGRCPSLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSVGSIMRLRAAAIKALS 601

Query: 616 QDSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSYHQHETRA 675
           QDSKDKST IF LAR FLYLSN QR+  ER N QS+KY R IEADDQGIS+SY Q+ETRA
Sbjct: 602 QDSKDKSTPIFQLARKFLYLSNYQRDVKERMNSQSEKYPRQIEADDQGISSSYQQNETRA 661

BLAST of Sgr021835 vs. ExPASy Swiss-Prot
Match: Q91WC9 (Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 1.2e-09
Identity = 71/303 (23.43%), Postives = 122/303 (40.26%), Query Frame = 0

Query: 366 AHSGIVEAARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAA 425
           AH GI +AAR ++ ++  +          G+LS       E   Y++ +VGHSLG   AA
Sbjct: 404 AHKGIAQAARYIHRRLVND----------GILSQAFSVAPE---YQLVLVGHSLGAGAAA 463

Query: 426 LLGLRLYRQCPNLHVYAYG-PLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRA 485
           LL + L    P +  YA+  P   +   +     +FV S++   +   RLS+ ++  L+ 
Sbjct: 464 LLAIMLRGAYPQVRAYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLSVTNMEDLKR 523

Query: 486 AAIKALSQDSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSY 545
             ++ ++  +K K  ++ H   G  Y       G+   +P  D +   ++   QG  T  
Sbjct: 524 RILRVIANCNKPKYKILLH---GCWY-------GLFGGSP--DNFPTELDEGTQGALTQ- 583

Query: 546 HQHETRANKKECQEFSLLAKNQTEDNVISIERNEFSNSDDLVSQFMEAIERSENDSTESF 605
                           LL     E  +++     + +SD            S  DS   +
Sbjct: 584 ---------------PLLG----EQTLLTRYSPGYCSSD------------SPLDSPTKY 640

Query: 606 SEMYLPGLLIHIVPQ--ERRFTLPFLNSLRGQPGADDYKAYVASRDGFKDLIVSPSMFLD 665
             +Y PG +IH+  +    RF         G   A  Y+A  A    F  +++ P M +D
Sbjct: 644 PTLYPPGRIIHLEEEGGSGRF---------GCCSAAQYRARWAHEAEFSKILIGPKMLID 640

BLAST of Sgr021835 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 6.1e-09
Identity = 75/332 (22.59%), Postives = 126/332 (37.95%), Query Frame = 0

Query: 341 EDVLTSNDHVHPNVKQKIRSSFPHYAHSGIVEAARDLYMQIEGNSRDDDGSEPCGLLSSL 400
           +DVLT       N++  I       AH GI +AAR +Y ++  +          G+LS  
Sbjct: 380 QDVLTDLSAESENLELDIELQ-DCVAHKGIAQAARYIYRRLVND----------GILSQA 439

Query: 401 VGAGHECDGYKVRIVGHSLGGAVAALLGLRLYRQCPNLHVYAYG-PLPCVDSIIASACSE 460
                E   Y++ +VGHSLG   AALL + L    P +  YA+  P   +   +     +
Sbjct: 440 FSVAPE---YRLVVVGHSLGAGAAALLAIMLRGAYPQVRAYAFSPPRGLLSKSLFEYSKD 499

Query: 461 FVTSIVFNNEFSSRLSIGSIMRLRAAAIKALSQDSKDKSTLIFHLARGFLYLSNCQRNGI 520
           FV S++   +   RLS+ ++  L+   ++ ++  +K K  ++ H   G  Y        +
Sbjct: 500 FVVSLILGMDVIPRLSVANMEDLKRRILRVIANCNKPKYKILLH---GCWY-------SV 559

Query: 521 ERKNPQSDKYSRHIEADDQGISTSYHQHE----TRANKKECQEFSLLAKNQTEDNVISIE 580
              +P  D +   ++  +QG  T     E    TR +   C   S L             
Sbjct: 560 FGGSP--DNFPTELDEGNQGALTQPLLGEQTLLTRCSPGYCSGDSPL------------- 619

Query: 581 RNEFSNSDDLVSQFMEAIERSENDSTESFSEMYLPGLLIHIVPQ--ERRFTLPFLNSLRG 640
                                    +  +  +Y PG +IH+  +    RF         G
Sbjct: 620 ------------------------DSPKYPTLYPPGRIIHLEEEGGSGRF---------G 639

Query: 641 QPGADDYKAYVASRDGFKDLIVSPSMFLDHLP 666
              A  Y+A  A    F  +++ P M +DH+P
Sbjct: 680 CCSAAQYRARWAHETEFSKILIGPKMLIDHMP 639

BLAST of Sgr021835 vs. ExPASy Swiss-Prot
Match: Q8NCG7 (Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 6.8e-08
Identity = 51/195 (26.15%), Postives = 83/195 (42.56%), Query Frame = 0

Query: 333 SYHLKYFEEDVLTSNDHVHPNVKQKIRSSF-----------------------PHYAHSG 392
           S+H K +E   L + DH   +V   +R +                           AH G
Sbjct: 348 SFHDKVYELPFLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDVECEVQDRLAHKG 407

Query: 393 IVEAARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLGL 452
           I +AAR +Y ++  +          G+LS       E   Y++ IVGHSLGG  AALL  
Sbjct: 408 ISQAARYVYQRLIND----------GILSQAFSIAPE---YRLVIVGHSLGGGAAALLAT 467

Query: 453 RLYRQCPNLHVYAYGPLPCVDSIIASACSE-FVTSIVFNNEFSSRLSIGSIMRLRAAAIK 504
            L    P +  YA+ P   + S      S+ F+ S+V   +   RLS+ ++  L+   ++
Sbjct: 468 MLRAAYPQVRCYAFSPPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRILR 527

BLAST of Sgr021835 vs. ExPASy Swiss-Prot
Match: Q9Y4D2 (Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3)

HSP 1 Score: 48.9 bits (115), Expect = 2.7e-04
Identity = 31/93 (33.33%), Postives = 46/93 (49.46%), Query Frame = 0

Query: 410 YKVRIVGHSLGGAVAALLGLRLYRQCPNLHVYAYGPLPCVDSIIASACS-EFVTSIVFNN 469
           Y + +VGHSLG   AA+L   L  Q P L  +AY P   + S  A   S EFVT++V   
Sbjct: 464 YGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGK 523

Query: 470 EFSSRLSIGSIMRLRAAAIKALSQDSKDKSTLI 502
           +   R+ +  +   R   +  L + +K K  +I
Sbjct: 524 DLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRII 556

BLAST of Sgr021835 vs. ExPASy Swiss-Prot
Match: Q6WQJ1 (Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 2.7e-04
Identity = 31/93 (33.33%), Postives = 46/93 (49.46%), Query Frame = 0

Query: 410 YKVRIVGHSLGGAVAALLGLRLYRQCPNLHVYAYGPLPCVDSIIASACS-EFVTSIVFNN 469
           Y + +VGHSLG   AA+L   L  Q P L  +AY P   + S  A   S EFVT++V   
Sbjct: 464 YGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGK 523

Query: 470 EFSSRLSIGSIMRLRAAAIKALSQDSKDKSTLI 502
           +   R+ +  +   R   +  L + +K K  +I
Sbjct: 524 DLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRII 556

BLAST of Sgr021835 vs. ExPASy TrEMBL
Match: A0A6J1D9V8 (sn1-specific diacylglycerol lipase beta isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018550 PE=4 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 1.2e-281
Identity = 534/787 (67.85%), Postives = 589/787 (74.84%), Query Frame = 0

Query: 14  MAQRRDQETSTFHEPLI-PSVCSSNHSNHQIRSPIPTFRSPWCPIRSRTEDMGV-SKLEN 73
           M +R  +E    HEPLI P  CS   S HQIRSPIP+ RSPWCPIRSRTE MG  SKL+N
Sbjct: 1   MERRIGEEICGLHEPLILPISCSI--SEHQIRSPIPSIRSPWCPIRSRTEAMGASSKLDN 60

Query: 74  IRCSTIVLAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIVTMVQCGIAQE 133
           IRCSTIVLAISNA V +LGA+L+IVV+PAC R+YVLPFLAVS+ASCI+IV MVQ GIAQE
Sbjct: 61  IRCSTIVLAISNAAVIILGAYLVIVVFPACGRRYVLPFLAVSVASCIRIVAMVQSGIAQE 120

Query: 134 ATARTILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAFYFIFHMASY 193
           ATARTILESPGD+ AVVNT++RHERRL YKRWLWWTRFALVIT+LQ VGA YFI +MA Y
Sbjct: 121 ATARTILESPGDSPAVVNTVLRHERRLSYKRWLWWTRFALVITMLQCVGAVYFIVNMAYY 180

Query: 194 IAHDDTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHA 253
           IAH +T  GCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHA
Sbjct: 181 IAHAETPGGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHA 240

Query: 254 WKAHYSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAG 313
           WKAHYSEIFD+GIREALCCLGR+KYLSVMEEDE+ SVAQLLGDLVAYRSSGTGHLEFLAG
Sbjct: 241 WKAHYSEIFDYGIREALCCLGRSKYLSVMEEDEVSSVAQLLGDLVAYRSSGTGHLEFLAG 300

Query: 314 -------------------SPYSMV-------------ITG------------------G 373
                              +P   +              TG                   
Sbjct: 301 LALLQRHGQSTQSSEELMEAPIDKIRDAAVLHKFAEAAYTGPLLDFGRNPCSFPCVWLYR 360

Query: 374 EAMLQPF------------------------------------LNMSSYHLKYF------ 433
           + ML P+                                    +N +     YF      
Sbjct: 361 QGMLTPWTRNKRPILHGDNWWRGHAAAFLKYVKLSPEVLRKGRVNQAKCEAAYFVLVLHD 420

Query: 434 -----------------------------EEDV--LTSNDHVHPNVKQKIRSSFPHYAHS 493
                                        EED+  L ++DHV PNVKQKI SSFPHYAHS
Sbjct: 421 VRCVVIAVRGTETPEDLITDGLCRECALSEEDLDGLMNSDHVQPNVKQKIISSFPHYAHS 480

Query: 494 GIVEAARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLG 553
           GIVEAARDLYMQIEGN +DD+GSEPCGLLSSL+G G ECDGY+VRIVGHSLGGA+AALLG
Sbjct: 481 GIVEAARDLYMQIEGNRKDDNGSEPCGLLSSLLGPGCECDGYEVRIVGHSLGGAIAALLG 540

Query: 554 LRLYRQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIK 613
           LRLYRQ PNLHVYAYGPLPCVDSIIASACSEFVTSIVF+NEFSSRLS GS+MRLRAAAIK
Sbjct: 541 LRLYRQFPNLHVYAYGPLPCVDSIIASACSEFVTSIVFSNEFSSRLSGGSVMRLRAAAIK 600

Query: 614 ALSQDSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSYHQHE 673
           ALSQDS DKST IF LAR FLYL+N QRNG E KN QSDKY+RHIEADD GIS+SY Q+E
Sbjct: 601 ALSQDSNDKSTQIFELARRFLYLNNQQRNGSEGKNSQSDKYARHIEADDLGISSSYQQNE 660

Query: 674 TRANKKECQEFSLLAKNQTEDNVISIERNEFSNSDDLVSQFMEAIERSENDS-TESFSEM 675
           TR NKKE QEFSLLA+NQTED++I++ERN+FSNSDDLVSQFMEAIERSEND  TESFS++
Sbjct: 661 TRENKKEGQEFSLLAENQTEDDIITVERNKFSNSDDLVSQFMEAIERSENDKPTESFSDV 720

BLAST of Sgr021835 vs. ExPASy TrEMBL
Match: A0A1S3BDV2 (sn1-specific diacylglycerol lipase beta isoform X4 OS=Cucumis melo OX=3656 GN=LOC103488587 PE=4 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 2.6e-273
Identity = 511/783 (65.26%), Postives = 573/783 (73.18%), Query Frame = 0

Query: 16  QRRDQETSTFHEPLIPSVCSSNHSNHQIRSPIPTFRSPWCPIRSRTEDMGVSKLENIRCS 75
           +RRDQE ST H+PLIPS    N SNHQIR PIPTF+SP CPIR+RTE MG SK++NIRCS
Sbjct: 2   ERRDQENSTLHQPLIPSDFPLN-SNHQIRLPIPTFQSPLCPIRNRTEAMGTSKMDNIRCS 61

Query: 76  TIVLAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIVTMVQCGIAQEATAR 135
           T VL ISNA +ALLG F++ V+YP+CER+Y LPFLAVS+ SCI+IVTMVQ GIAQEATAR
Sbjct: 62  TFVLGISNAAIALLGGFIIFVLYPSCERRYTLPFLAVSLVSCIRIVTMVQSGIAQEATAR 121

Query: 136 TILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAFYFIFHMASYIAHD 195
           TILESPGDTAAV++T+MR ERRLRYKRWLWWTRFAL++ ++QF GA Y IFHM ++I HD
Sbjct: 122 TILESPGDTAAVIDTVMRRERRLRYKRWLWWTRFALILVMMQFAGAVYLIFHMTNHIGHD 181

Query: 196 DTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHAWKAH 255
           +TSSGCALGVAS+DRW +RKLLVLFTILVCFVALVQCFTGMDVLRWRSFY TQDHAWKAH
Sbjct: 182 ETSSGCALGVASHDRWLKRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYSTQDHAWKAH 241

Query: 256 YSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAGSPY- 315
           YSEIFDHGIRE LCCLGR+KYLSVMEEDE+FSVAQLLGDLV YRS+GTGHLEFLAG    
Sbjct: 242 YSEIFDHGIREVLCCLGRSKYLSVMEEDEVFSVAQLLGDLVTYRSTGTGHLEFLAGLALL 301

Query: 316 ----SMVITGGEAMLQPF------------------------------------------ 375
                +V T  E M  P                                           
Sbjct: 302 QRHGQLVHTSEELMEAPIDKIEEAAVLHKFAEAAYTGPLLDFGRNPLSFPCAWVYRQGIL 361

Query: 376 ---------------------------------------LNMSSYHLKYF---------- 435
                                                  +N +     YF          
Sbjct: 362 TPWTRNKRPVLHGDNWWRGHAAAFLKYVKLPPEVLRRGRVNQAKCEAAYFVLVLHDVKCV 421

Query: 436 -------------------------EEDV--LTSNDHVHPNVKQKIRSSFPHYAHSGIVE 495
                                    EED+  L + DH+  +VKQ+I SS PH+AHSGIVE
Sbjct: 422 VIAVRGTETPEDLITDGLCREYTLTEEDLDGLINCDHIQLSVKQRIISSSPHHAHSGIVE 481

Query: 496 AARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLGLRLY 555
           AAR+LYMQIEGN +D DGSEPCGLLSSL+G G ECDGY+VRIVGHSLGGA+AALLGLRLY
Sbjct: 482 AARELYMQIEGNCKDHDGSEPCGLLSSLLGPGCECDGYQVRIVGHSLGGAIAALLGLRLY 541

Query: 556 RQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIKALSQ 615
            +CP+LHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLS+GSIMRLRAAAIKALSQ
Sbjct: 542 GRCPSLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSVGSIMRLRAAAIKALSQ 601

Query: 616 DSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSYHQHETRAN 675
           DSKDKST IF LAR FLYLSN QR+  ER N QS+KY R IEADDQGIS+SY Q+ETRA 
Sbjct: 602 DSKDKSTPIFQLARKFLYLSNYQRDVKERMNSQSEKYPRQIEADDQGISSSYQQNETRAI 661

BLAST of Sgr021835 vs. ExPASy TrEMBL
Match: A0A1S4DWQ3 (sn1-specific diacylglycerol lipase beta isoform X3 OS=Cucumis melo OX=3656 GN=LOC103488587 PE=4 SV=1)

HSP 1 Score: 948.7 bits (2451), Expect = 1.3e-272
Identity = 511/784 (65.18%), Postives = 573/784 (73.09%), Query Frame = 0

Query: 16  QRRDQETSTFHEPLIPSVCSSNHSNHQIRSPIPTFRSPWCPIRSRTEDMGVSKLENIRCS 75
           +RRDQE ST H+PLIPS    N SNHQIR PIPTF+SP CPIR+RTE MG SK++NIRCS
Sbjct: 2   ERRDQENSTLHQPLIPSDFPLN-SNHQIRLPIPTFQSPLCPIRNRTEAMGTSKMDNIRCS 61

Query: 76  TIVLAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIVTMVQCGIAQEATAR 135
           T VL ISNA +ALLG F++ V+YP+CER+Y LPFLAVS+ SCI+IVTMVQ GIAQEATAR
Sbjct: 62  TFVLGISNAAIALLGGFIIFVLYPSCERRYTLPFLAVSLVSCIRIVTMVQSGIAQEATAR 121

Query: 136 TILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAFYFIFHMASYIAHD 195
           TILESPGDTAAV++T+MR ERRLRYKRWLWWTRFAL++ ++QF GA Y IFHM ++I HD
Sbjct: 122 TILESPGDTAAVIDTVMRRERRLRYKRWLWWTRFALILVMMQFAGAVYLIFHMTNHIGHD 181

Query: 196 DTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHAWKAH 255
           +TSSGCALGVAS+DRW +RKLLVLFTILVCFVALVQCFTGMDVLRWRSFY TQDHAWKAH
Sbjct: 182 ETSSGCALGVASHDRWLKRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYSTQDHAWKAH 241

Query: 256 YSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAGSPY- 315
           YSEIFDHGIRE LCCLGR+KYLSVMEEDE+FSVAQLLGDLV YRS+GTGHLEFLAG    
Sbjct: 242 YSEIFDHGIREVLCCLGRSKYLSVMEEDEVFSVAQLLGDLVTYRSTGTGHLEFLAGLALL 301

Query: 316 ----SMVITGGEAMLQPF------------------------------------------ 375
                +V T  E M  P                                           
Sbjct: 302 QRHGQLVHTSEELMEAPIDKIEEAAVLHKFAEAAYTGPLLDFGRNPLSFPCAWVYRQGIL 361

Query: 376 ---------------------------------------LNMSSYHLKYF---------- 435
                                                  +N +     YF          
Sbjct: 362 TPWTRNKRPVLHGDNWWRGHAAAFLKYVKLPPEVLRRGRVNQAKCEAAYFVLVLHDVKCV 421

Query: 436 ---------EEDVLTS-----------------NDHVHPNVKQKIRSSFPHYAHSGIVEA 495
                     ED++T                  NDH+  +VKQ+I SS PH+AHSGIVEA
Sbjct: 422 VIAVRGTETPEDLITDGLCREYTLTEEDLDGLINDHIQLSVKQRIISSSPHHAHSGIVEA 481

Query: 496 ARDLYMQIEGNSRDDD--GSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLGLRL 555
           AR+LYMQIEGN +D D  GSEPCGLLSSL+G G ECDGY+VRIVGHSLGGA+AALLGLRL
Sbjct: 482 ARELYMQIEGNCKDHDGAGSEPCGLLSSLLGPGCECDGYQVRIVGHSLGGAIAALLGLRL 541

Query: 556 YRQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIKALS 615
           Y +CP+LHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLS+GSIMRLRAAAIKALS
Sbjct: 542 YGRCPSLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSVGSIMRLRAAAIKALS 601

Query: 616 QDSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSYHQHETRA 675
           QDSKDKST IF LAR FLYLSN QR+  ER N QS+KY R IEADDQGIS+SY Q+ETRA
Sbjct: 602 QDSKDKSTPIFQLARKFLYLSNYQRDVKERMNSQSEKYPRQIEADDQGISSSYQQNETRA 661

BLAST of Sgr021835 vs. ExPASy TrEMBL
Match: A0A1S3BD53 (sn1-specific diacylglycerol lipase beta isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488587 PE=4 SV=1)

HSP 1 Score: 946.0 bits (2444), Expect = 8.5e-272
Identity = 511/785 (65.10%), Postives = 573/785 (72.99%), Query Frame = 0

Query: 16  QRRDQETSTFHEPLIPSVCSSNHSNHQIRSPIPTFRSPWCPIRSRTEDMGVSKLENIRCS 75
           +RRDQE ST H+PLIPS    N SNHQIR PIPTF+SP CPIR+RTE MG SK++NIRCS
Sbjct: 2   ERRDQENSTLHQPLIPSDFPLN-SNHQIRLPIPTFQSPLCPIRNRTEAMGTSKMDNIRCS 61

Query: 76  TIVLAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIVTMVQCGIAQEATAR 135
           T VL ISNA +ALLG F++ V+YP+CER+Y LPFLAVS+ SCI+IVTMVQ GIAQEATAR
Sbjct: 62  TFVLGISNAAIALLGGFIIFVLYPSCERRYTLPFLAVSLVSCIRIVTMVQSGIAQEATAR 121

Query: 136 TILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAFYFIFHMASYIAHD 195
           TILESPGDTAAV++T+MR ERRLRYKRWLWWTRFAL++ ++QF GA Y IFHM ++I HD
Sbjct: 122 TILESPGDTAAVIDTVMRRERRLRYKRWLWWTRFALILVMMQFAGAVYLIFHMTNHIGHD 181

Query: 196 DTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHAWKAH 255
           +TSSGCALGVAS+DRW +RKLLVLFTILVCFVALVQCFTGMDVLRWRSFY TQDHAWKAH
Sbjct: 182 ETSSGCALGVASHDRWLKRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYSTQDHAWKAH 241

Query: 256 YSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAGSPY- 315
           YSEIFDHGIRE LCCLGR+KYLSVMEEDE+FSVAQLLGDLV YRS+GTGHLEFLAG    
Sbjct: 242 YSEIFDHGIREVLCCLGRSKYLSVMEEDEVFSVAQLLGDLVTYRSTGTGHLEFLAGLALL 301

Query: 316 ----SMVITGGEAMLQPF------------------------------------------ 375
                +V T  E M  P                                           
Sbjct: 302 QRHGQLVHTSEELMEAPIDKIEEAAVLHKFAEAAYTGPLLDFGRNPLSFPCAWVYRQGIL 361

Query: 376 ---------------------------------------LNMSSYHLKYF---------- 435
                                                  +N +     YF          
Sbjct: 362 TPWTRNKRPVLHGDNWWRGHAAAFLKYVKLPPEVLRRGRVNQAKCEAAYFVLVLHDVKCV 421

Query: 436 -------------------------EEDV--LTSNDHVHPNVKQKIRSSFPHYAHSGIVE 495
                                    EED+  L + DH+  +VKQ+I SS PH+AHSGIVE
Sbjct: 422 VIAVRGTETPEDLITDGLCREYTLTEEDLDGLINCDHIQLSVKQRIISSSPHHAHSGIVE 481

Query: 496 AARDLYMQIEGNSRDDD--GSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLGLR 555
           AAR+LYMQIEGN +D D  GSEPCGLLSSL+G G ECDGY+VRIVGHSLGGA+AALLGLR
Sbjct: 482 AARELYMQIEGNCKDHDGAGSEPCGLLSSLLGPGCECDGYQVRIVGHSLGGAIAALLGLR 541

Query: 556 LYRQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIKAL 615
           LY +CP+LHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLS+GSIMRLRAAAIKAL
Sbjct: 542 LYGRCPSLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSVGSIMRLRAAAIKAL 601

Query: 616 SQDSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSYHQHETR 675
           SQDSKDKST IF LAR FLYLSN QR+  ER N QS+KY R IEADDQGIS+SY Q+ETR
Sbjct: 602 SQDSKDKSTPIFQLARKFLYLSNYQRDVKERMNSQSEKYPRQIEADDQGISSSYQQNETR 661

BLAST of Sgr021835 vs. ExPASy TrEMBL
Match: A0A6J1EVF0 (sn1-specific diacylglycerol lipase beta isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436326 PE=4 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.5e-271
Identity = 507/780 (65.00%), Postives = 571/780 (73.21%), Query Frame = 0

Query: 19  DQETSTFHEPLIPSVCSSNHSNHQIRSPIPTFRSPWCPIRSRTEDMGVSKLENIRCSTIV 78
           D   S  HEPLIPS   SN S++QI SP+PTF SPWCPIRSRTE MG SKLENIR STIV
Sbjct: 2   DHGNSDIHEPLIPSDSPSN-SSYQIPSPVPTFPSPWCPIRSRTEAMGASKLENIRFSTIV 61

Query: 79  LAISNALVALLGAFLLIVVYPACERQYVLPFLAVSMASCIKIVTMVQCGIAQEATARTIL 138
           L ISNA VALLG FL++VVYPACERQY+LPF+ VS+ SCI+IV MVQ GIAQEATARTIL
Sbjct: 62  LGISNAAVALLGVFLVVVVYPACERQYILPFVGVSVVSCIRIVAMVQSGIAQEATARTIL 121

Query: 139 ESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAFYFIFHMASYIAHDDTS 198
           ESPGDTAAVV+T+MR ERRLRYK+WLWWTRFALVI +LQ  GA Y I+HMA+YIAHD+TS
Sbjct: 122 ESPGDTAAVVDTVMRRERRLRYKKWLWWTRFALVIVMLQLGGAVYLIYHMANYIAHDETS 181

Query: 199 SGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWRSFYETQDHAWKAHYSE 258
           +GCAL VASN RWW+RKLLVLFTILVCFVALVQCF GMDVLRWRSFY TQDHAWKAHYSE
Sbjct: 182 TGCALVVASNQRWWKRKLLVLFTILVCFVALVQCFAGMDVLRWRSFYATQDHAWKAHYSE 241

Query: 259 IFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAG------- 318
           +FDHGIREALCC+GR+KYLSVMEEDE+FSVAQLLGDLVAYRSSGTGH EFLAG       
Sbjct: 242 MFDHGIREALCCMGRSKYLSVMEEDEVFSVAQLLGDLVAYRSSGTGHFEFLAGLALLQRH 301

Query: 319 ---------------------------------------------SPYSMVITGG----- 378
                                                         P + V   G     
Sbjct: 302 GQQVQISEELMEAPIDKIEEAAVLHKFAEAAYTGPLLDFGRNPLLFPCAWVYRQGIFTPW 361

Query: 379 -----------------------------EAMLQPFLNMSSYHLKYF------------- 438
                                        E + +  +N S     YF             
Sbjct: 362 TRNKRPVLHGDNWWRGHAAAFLKYVKLSPEVLRRGRVNQSKCEAAYFVLVLHDVKCVVIA 421

Query: 439 ------EEDVLTS-----------------NDHVHPNVKQKIRSSFPHYAHSGIVEAARD 498
                  ED++T                  N  + PNVKQ+I SSFPH+AHSGIVEAARD
Sbjct: 422 VRGTETPEDLITDGLCRECALSEEDLDGLINGDIQPNVKQRIMSSFPHHAHSGIVEAARD 481

Query: 499 LYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVAALLGLRLYRQCP 558
           LYMQIEGN RD+DGSEPCGLLSSL+G G ECDGY+VRIVGHSLGGA+AALLGLRLY +CP
Sbjct: 482 LYMQIEGNCRDNDGSEPCGLLSSLLGPGCECDGYQVRIVGHSLGGAIAALLGLRLYSRCP 541

Query: 559 NLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIKALSQDSKD 618
           +LHVYAYGPLPCVDSIIASACS+FVTSIVFNNEFSSRLS+GSI+RLRAAAIKALS+DSKD
Sbjct: 542 SLHVYAYGPLPCVDSIIASACSDFVTSIVFNNEFSSRLSVGSILRLRAAAIKALSKDSKD 601

Query: 619 KSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGISTSYHQHETRANKKEC 675
           KST +F LAR FLY+S  Q++G ERKN QS+KY R I+ADDQ ISTSY Q+E+R +K EC
Sbjct: 602 KSTPVFELARRFLYVS--QKDGTERKNSQSEKYPREIQADDQRISTSYQQNESRVDKNEC 661

BLAST of Sgr021835 vs. TAIR 10
Match: AT1G05790.1 (lipase class 3 family protein )

HSP 1 Score: 427.9 bits (1099), Expect = 1.5e-119
Identity = 285/733 (38.88%), Postives = 375/733 (51.16%), Query Frame = 0

Query: 64  MGVSKLENIRCSTIVLAISNALVALLGAFLLIVVYPACERQ-YVLPFLAVSMASCIKIVT 123
           M +S+L+ +R + +VL ++N +V + G  L +V    C+    + P  AV  A+ +K+  
Sbjct: 1   MWISRLKRVRRTIMVLGVANFVVIVSGCVLTLVSDADCDSPGQLFPLFAVCFAAGVKLAA 60

Query: 124 MVQCGIAQEATARTILESPGDTAAVVNTIMRHERRLRYKRWLWWTRFALVITVLQFVGAF 183
           MV+ G  QE  A TI++SP        T   H+R+L+YK WLWWTRFA+VITVLQF+GA 
Sbjct: 61  MVKVGTTQELMAMTIMDSP--------TQNNHQRKLKYKTWLWWTRFAMVITVLQFIGAT 120

Query: 184 YFIFHMASYIAHDDTSSGCALGVASNDRWWRRKLLVLFTILVCFVALVQCFTGMDVLRWR 243
           Y +F +A Y++ D     C LG++ +   W++ L V F I VCFVAL QCFTG D+L+WR
Sbjct: 121 YLLFRLAKYVSRDGLPRNCVLGLSPDTGGWKQTLQVTFLITVCFVALAQCFTGSDILQWR 180

Query: 244 SFYETQDHAWKAHYSEIFDHGIREALCCLGRAKYLSVMEEDEIFSVAQLLGDLVAYRSSG 303
                                               V+EEDE+ SVA+LLGDLV+YR+SG
Sbjct: 181 -----------------------------------GVIEEDEVCSVARLLGDLVSYRASG 240

Query: 304 TGHLEFLAG------------------------------------------------SPY 363
           TGHLEFLAG                                                +P 
Sbjct: 241 TGHLEFLAGLALLQSNSQFPESYEDCMEAPAFHLQEAAMLHKFAEAAYTGPLLDVGRNPA 300

Query: 364 SMVIT--------------------------GGEAMLQPFLNMSSYHLK----------- 423
             + T                          G  A    F+N  ++ L+           
Sbjct: 301 LFLCTWICRQGILTPWSRKWRPKLDGDNWWRGHAAAFLKFINFPAHVLRRGRICREKCKA 360

Query: 424 -YF-------------------EEDVLTS----------------NDHVHPNVKQKIRSS 483
            YF                    ED++T                  +HVH      + +S
Sbjct: 361 TYFVVVLHYLRCVVIAVRGTETAEDLITDGLGRACSLTVEDLDGLTNHVH-----GMDTS 420

Query: 484 FPHYAHSGIVEAARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGG 543
             HY HSGIVEAARDL+MQIEG+ +  + SE  G LSSL+G G ECDGY +RIVGHSLGG
Sbjct: 421 RKHYGHSGIVEAARDLFMQIEGDPKSGE-SESSGFLSSLIGDGCECDGYSIRIVGHSLGG 480

Query: 544 AVAALLGLRLYRQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMR 603
           A+A+LLG+RL  + PNL+VYAYGPLPCVDS +A ACSEFVTSIV +NEFSSRLS GSI R
Sbjct: 481 AIASLLGIRLRCRFPNLYVYAYGPLPCVDSDVAEACSEFVTSIVLDNEFSSRLSYGSIRR 540

Query: 604 LRAAAIKALSQDSKDKSTLIFHLARGFLYLSNCQRNGIERKNPQSDKYSRHIEADDQGIS 663
           L+ AAIK LSQD K  + LIF LAR FL  S  QR  +E K  +        EA D   S
Sbjct: 541 LQVAAIKVLSQDPKADTALIFRLARRFLSASKRQRENVEEKTSE--------EAIDVNNS 600

Query: 664 TSYHQHETRANKKECQEFSLLAKNQTEDNVISIERNEFSNSDDLVSQFMEAIERSENDST 675
                 +     +E +     A+ Q +        +  ++ D+ VSQFME     E+D  
Sbjct: 601 PESQHDQIYPIWEEAE-----AEMQQDSEEFINPFHGMASEDNPVSQFMETGPTKEDD-- 660

BLAST of Sgr021835 vs. TAIR 10
Match: AT1G05790.2 (lipase class 3 family protein )

HSP 1 Score: 301.2 bits (770), Expect = 2.1e-81
Identity = 207/519 (39.88%), Postives = 263/519 (50.67%), Query Frame = 0

Query: 277 LSVMEEDEIFSVAQLLGDLVAYRSSGTGHLEFLAG------------------------- 336
           + V+EEDE+ SVA+LLGDLV+YR+SGTGHLEFLAG                         
Sbjct: 1   MGVIEEDEVCSVARLLGDLVSYRASGTGHLEFLAGLALLQSNSQFPESYEDCMEAPAFHL 60

Query: 337 -----------------------SPYSMVIT--------------------------GGE 396
                                  +P   + T                          G  
Sbjct: 61  QEAAMLHKFAEAAYTGPLLDVGRNPALFLCTWICRQGILTPWSRKWRPKLDGDNWWRGHA 120

Query: 397 AMLQPFLNMSSYHLK------------YF-------------------EEDVLTS----- 456
           A    F+N  ++ L+            YF                    ED++T      
Sbjct: 121 AAFLKFINFPAHVLRRGRICREKCKATYFVVVLHYLRCVVIAVRGTETAEDLITDGLGRA 180

Query: 457 -----------NDHVHPNVKQKIRSSFPHYAHSGIVEAARDLYMQIEGNSRDDDGSEPCG 516
                       +HVH      + +S  HY HSGIVEAARDL+MQIEG+ +  + SE  G
Sbjct: 181 CSLTVEDLDGLTNHVH-----GMDTSRKHYGHSGIVEAARDLFMQIEGDPKSGE-SESSG 240

Query: 517 LLSSLVGAGHECDGYKVRIVGHSLGGAVAALLGLRLYRQCPNLHVYAYGPLPCVDSIIAS 576
            LSSL+G G ECDGY +RIVGHSLGGA+A+LLG+RL  + PNL+VYAYGPLPCVDS +A 
Sbjct: 241 FLSSLIGDGCECDGYSIRIVGHSLGGAIASLLGIRLRCRFPNLYVYAYGPLPCVDSDVAE 300

Query: 577 ACSEFVTSIVFNNEFSSRLSIGSIMRLRAAAIKALSQDSKDKSTLIFHLARGFLYLSNCQ 636
           ACSEFVTSIV +NEFSSRLS GSI RL+ AAIK LSQD K  + LIF LAR FL  S  Q
Sbjct: 301 ACSEFVTSIVLDNEFSSRLSYGSIRRLQVAAIKVLSQDPKADTALIFRLARRFLSASKRQ 360

Query: 637 RNGIERKNPQSDKYSRHIEADDQGISTSYHQHETRANKKECQEFSLLAKNQTEDNVISIE 675
           R  +E K  +        EA D   S      +     +E +     A+ Q +       
Sbjct: 361 RENVEEKTSE--------EAIDVNNSPESQHDQIYPIWEEAE-----AEMQQDSEEFINP 420

BLAST of Sgr021835 vs. TAIR 10
Match: AT2G42450.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 8.8e-11
Identity = 35/84 (41.67%), Postives = 49/84 (58.33%), Query Frame = 0

Query: 408 DGYKVRIVGHSLGGAVAALLGLRLYR--------QCPNLHVYAYGPLPCVDSIIASACSE 467
           +GYK+R+VGHSLGGA+A+L+ + L +            +    Y   PCV   +A  CSE
Sbjct: 301 EGYKLRLVGHSLGGAIASLMAIMLKKMPREELGFDAEIISAVGYATPPCVSKELAENCSE 360

Query: 468 FVTSIVFNNEFSSRLSIGSIMRLR 484
           FVT+IV  ++   RLS  S+ RLR
Sbjct: 361 FVTTIVMQDDIIPRLSAASLARLR 384

BLAST of Sgr021835 vs. TAIR 10
Match: AT4G16070.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 53.5 bits (127), Expect = 7.7e-07
Identity = 42/136 (30.88%), Postives = 66/136 (48.53%), Query Frame = 0

Query: 365 YAHSGIVEAARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVA 424
           YAH G+V AAR +             S PC     L+ A  E   +KV+IVGHSLGG  A
Sbjct: 221 YAHCGMVAAARWIAKL----------SVPC-----LLKALDENPSFKVQIVGHSLGGGTA 280

Query: 425 ALLG--LRLYRQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRL 484
           +LL   LR  ++  +   + + P  C+   +A +   F+T+I+  ++     S  S+  L
Sbjct: 281 SLLTYILREQKEFASATCFTFAPAACMTWDLAESGKHFITTIINGSDLVPTFSASSVDDL 340

Query: 485 RA-AAIKALSQDSKDK 498
           R+     + S D +D+
Sbjct: 341 RSEVTSSSWSNDLRDQ 341

BLAST of Sgr021835 vs. TAIR 10
Match: AT3G14075.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 49.7 bits (117), Expect = 1.1e-05
Identity = 37/122 (30.33%), Postives = 57/122 (46.72%), Query Frame = 0

Query: 365 YAHSGIVEAARDLYMQIEGNSRDDDGSEPCGLLSSLVGAGHECDGYKVRIVGHSLGGAVA 424
           YAH G+V AAR +             + PC LL  L     +   YK++IVGHSLGG  A
Sbjct: 223 YAHCGMVAAARCIAKL----------ATPC-LLKGL----EQYPDYKIKIVGHSLGGGTA 282

Query: 425 ALLG--LRLYRQCPNLHVYAYGPLPCVDSIIASACSEFVTSIVFNNEFSSRLSIGSIMRL 484
           ALL   +R  +         + P  C+   +A + ++F+ S++   +     S  ++  L
Sbjct: 283 ALLTYIMREQKMLSTATCVTFAPAACMTWELADSGNDFIVSVINGADLVPTFSAAAVDDL 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150369.12.4e-28167.85sn1-specific diacylglycerol lipase beta isoform X1 [Momordica charantia][more]
KAG6575339.12.6e-27563.39Sn1-specific diacylglycerol lipase beta, partial [Cucurbita argyrosperma subsp. ... [more]
XP_038884281.11.0e-27465.64diacylglycerol lipase-beta isoform X4 [Benincasa hispida][more]
XP_008445624.15.5e-27365.26PREDICTED: sn1-specific diacylglycerol lipase beta isoform X4 [Cucumis melo][more]
XP_016900150.12.7e-27265.18PREDICTED: sn1-specific diacylglycerol lipase beta isoform X3 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q91WC91.2e-0923.43Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2[more]
P0C1S96.1e-0922.59Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1[more]
Q8NCG76.8e-0826.15Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2[more]
Q9Y4D22.7e-0433.33Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3[more]
Q6WQJ12.7e-0433.33Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1D9V81.2e-28167.85sn1-specific diacylglycerol lipase beta isoform X1 OS=Momordica charantia OX=367... [more]
A0A1S3BDV22.6e-27365.26sn1-specific diacylglycerol lipase beta isoform X4 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A1S4DWQ31.3e-27265.18sn1-specific diacylglycerol lipase beta isoform X3 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A1S3BD538.5e-27265.10sn1-specific diacylglycerol lipase beta isoform X1 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1EVF02.5e-27165.00sn1-specific diacylglycerol lipase beta isoform X2 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT1G05790.11.5e-11938.88lipase class 3 family protein [more]
AT1G05790.22.1e-8139.88lipase class 3 family protein [more]
AT2G42450.18.8e-1141.67alpha/beta-Hydrolases superfamily protein [more]
AT4G16070.17.7e-0730.88Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT3G14075.11.1e-0530.33Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 406..462
e-value: 1.9E-7
score: 31.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 334..558
e-value: 2.0E-17
score: 65.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 366..474
NoneNo IPR availablePANTHERPTHR47030LIPASE CLASS 3 FAMILY PROTEINcoord: 342..675
coord: 66..311
NoneNo IPR availableCDDcd00519Lipase_3coord: 364..479
e-value: 1.40363E-18
score: 83.2946

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr021835.1Sgr021835.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016020 membrane