Sgr021616 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr021616
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionethylene-responsive transcription factor CRF2-like
Locationtig00153764: 633965 .. 634855 (-)
RNA-Seq ExpressionSgr021616
SyntenySgr021616
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGTTCCGAGCCGCAATCCCACCATGAAACCGCCAGAAAAGTCCGCCGTCCGGAACTCTCCCGGTTTCAGAATTGTTCGGATTTCCATGACCGATCCAGATGCTACCGATTCGTCCAGCGACGAAGAACCGGCGGTTTTCGCCCCCCGCCGTGTGAAGCGGTACGTTGACGAGGTCACCATCGATACGGCCGCGGAAAGCCGGGGTGGTCGCCGGAAACGTAAGGCGGACAAAGCTGTGCGTCGGCGGAGGAAGAAGAAGAAGTCGCCCGGTAATGTGATGAAGTTTCGAGGTGTCCGGCGGCGGCCGTGGGGGAAATGGGCGGCGGAGATTCGAGACTCGGCCCGCCGCGTAAGGCTGTGGTTGGGGACTTACGACACGGCGGAGGAGGCCGCCATGGTGTACGATAACGCCGCCATTAAGCTCCGAGGACCCGACACTTTAACGAACTTCGCCACCCCTCCGCCGCCATTCTCCGGCGAAGAGTCGTCTCCGTCTCAGTCTCCGACGTCGGTCCTCCAATACAGAAGTCACTTCACCGAATCAGAACCAGAGCAGCCAAAAGAATGCTCGCAACCGTGTCGAGCTACCGGAACCGCCGGCTCCCCCGACGAATCTCCCGTCGTCGATTACCCGCCGCTCGACTTCCCCTGCTCCGACGACATATTCAAATCAATCCTCTCCGATTCTCCTCTGTTTGTCGACTACCAAACAACCCTCGTACCAGAAACTCCATGGACGGACAGCGGCGGCGACTGCTCCGGCTCCGGCGACATTCTCAAAAACATACATCAGATTCTGTACTCGCCGGCGGCGGGGACCGGGCAAGGAGGAGATGACTACTTCCAAGACATATTGATGGGGTCAGATCCTCTGGTGGTCCTATGA

mRNA sequence

ATGAATGTTCCGAGCCGCAATCCCACCATGAAACCGCCAGAAAAGTCCGCCGTCCGGAACTCTCCCGGTTTCAGAATTGTTCGGATTTCCATGACCGATCCAGATGCTACCGATTCGTCCAGCGACGAAGAACCGGCGGTTTTCGCCCCCCGCCGTGTGAAGCGGTACGTTGACGAGGTCACCATCGATACGGCCGCGGAAAGCCGGGGTGGTCGCCGGAAACGTAAGGCGGACAAAGCTGTGCGTCGGCGGAGGAAGAAGAAGAAGTCGCCCGGTAATGTGATGAAGTTTCGAGGTGTCCGGCGGCGGCCGTGGGGGAAATGGGCGGCGGAGATTCGAGACTCGGCCCGCCGCGTAAGGCTGTGGTTGGGGACTTACGACACGGCGGAGGAGGCCGCCATGGTGTACGATAACGCCGCCATTAAGCTCCGAGGACCCGACACTTTAACGAACTTCGCCACCCCTCCGCCGCCATTCTCCGGCGAAGAGTCGTCTCCGTCTCAGTCTCCGACGTCGGTCCTCCAATACAGAAGTCACTTCACCGAATCAGAACCAGAGCAGCCAAAAGAATGCTCGCAACCGTGTCGAGCTACCGGAACCGCCGGCTCCCCCGACGAATCTCCCGTCGTCGATTACCCGCCGCTCGACTTCCCCTGCTCCGACGACATATTCAAATCAATCCTCTCCGATTCTCCTCTGTTTGTCGACTACCAAACAACCCTCGTACCAGAAACTCCATGGACGGACAGCGGCGGCGACTGCTCCGGCTCCGGCGACATTCTCAAAAACATACATCAGATTCTGTACTCGCCGGCGGCGGGGACCGGGCAAGGAGGAGATGACTACTTCCAAGACATATTGATGGGGTCAGATCCTCTGGTGGTCCTATGA

Coding sequence (CDS)

ATGAATGTTCCGAGCCGCAATCCCACCATGAAACCGCCAGAAAAGTCCGCCGTCCGGAACTCTCCCGGTTTCAGAATTGTTCGGATTTCCATGACCGATCCAGATGCTACCGATTCGTCCAGCGACGAAGAACCGGCGGTTTTCGCCCCCCGCCGTGTGAAGCGGTACGTTGACGAGGTCACCATCGATACGGCCGCGGAAAGCCGGGGTGGTCGCCGGAAACGTAAGGCGGACAAAGCTGTGCGTCGGCGGAGGAAGAAGAAGAAGTCGCCCGGTAATGTGATGAAGTTTCGAGGTGTCCGGCGGCGGCCGTGGGGGAAATGGGCGGCGGAGATTCGAGACTCGGCCCGCCGCGTAAGGCTGTGGTTGGGGACTTACGACACGGCGGAGGAGGCCGCCATGGTGTACGATAACGCCGCCATTAAGCTCCGAGGACCCGACACTTTAACGAACTTCGCCACCCCTCCGCCGCCATTCTCCGGCGAAGAGTCGTCTCCGTCTCAGTCTCCGACGTCGGTCCTCCAATACAGAAGTCACTTCACCGAATCAGAACCAGAGCAGCCAAAAGAATGCTCGCAACCGTGTCGAGCTACCGGAACCGCCGGCTCCCCCGACGAATCTCCCGTCGTCGATTACCCGCCGCTCGACTTCCCCTGCTCCGACGACATATTCAAATCAATCCTCTCCGATTCTCCTCTGTTTGTCGACTACCAAACAACCCTCGTACCAGAAACTCCATGGACGGACAGCGGCGGCGACTGCTCCGGCTCCGGCGACATTCTCAAAAACATACATCAGATTCTGTACTCGCCGGCGGCGGGGACCGGGCAAGGAGGAGATGACTACTTCCAAGACATATTGATGGGGTCAGATCCTCTGGTGGTCCTATGA

Protein sequence

MNVPSRNPTMKPPEKSAVRNSPGFRIVRISMTDPDATDSSSDEEPAVFAPRRVKRYVDEVTIDTAAESRGGRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDTLTNFATPPPPFSGEESSPSQSPTSVLQYRSHFTESEPEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPLFVDYQTTLVPETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQGGDDYFQDILMGSDPLVVL
Homology
BLAST of Sgr021616 vs. NCBI nr
Match: XP_022157129.1 (ethylene-responsive transcription factor CRF2-like [Momordica charantia])

HSP 1 Score: 308.5 bits (789), Expect = 6.1e-80
Identity = 193/305 (63.28%), Postives = 216/305 (70.82%), Query Frame = 0

Query: 1   MNVPS---RNPTMKPPEKSAVRNSPGFRIVRISMTDPDATDSSSDEEPA-VFAPRRVKRY 60
           M+VP+   R PT KPPE     NS G RIVRIS+TDPDATDSSSD+EPA +   RR+K Y
Sbjct: 1   MDVPTNLRRKPTTKPPE-----NSAGSRIVRISITDPDATDSSSDDEPAMLLTRRRLKCY 60

Query: 61  VDEVTIDTAAESRGGRRKRKADKAVRRRRK-KKKSPGNVMKFRGVRRRPWGKWAAEIRDS 120
           VDEVTI+T AE+ GGRRKR A+  VRRRR+ KKKSPGNV KF GVRRRPWGKW     DS
Sbjct: 61  VDEVTIET-AETGGGRRKRTANGLVRRRRRMKKKSPGNVGKFLGVRRRPWGKW-----DS 120

Query: 121 ARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDTLTNFAT--PPPPFSGEESSPSQSPTSV 180
             R+RLW GTYDTAE+      NAA+K RGP  LTNF T  PPPPF GE+S  S SPTSV
Sbjct: 121 GCRIRLWFGTYDTAED-----KNAAMKFRGPAVLTNFPTPPPPPPFPGEDS--SSSPTSV 180

Query: 181 LQYRSHFTESEPEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPL 240
           L Y +HFTES+PE P     PCR T T G   ESPVV     DFPCSDDIFKSIL +SPL
Sbjct: 181 LHYGTHFTESKPEPP-----PCRTTATFGVVAESPVV-----DFPCSDDIFKSILFESPL 240

Query: 241 FVDYQTTLVPETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQGGD--DYFQDILMGSD 297
           F DYQT LVPETPWTD       SG       + LYS AAG+GQGG+  D+F++ILMGSD
Sbjct: 241 FFDYQTNLVPETPWTD-----GSSG-------EFLYSAAAGSGQGGEEHDFFEEILMGSD 265

BLAST of Sgr021616 vs. NCBI nr
Match: XP_038875714.1 (ethylene-responsive transcription factor CRF1 [Benincasa hispida])

HSP 1 Score: 281.6 bits (719), Expect = 8.0e-72
Identity = 179/306 (58.50%), Postives = 209/306 (68.30%), Query Frame = 0

Query: 1   MNVPS---RNPTMKPPEKSAVRNSPGFRIVRISMTDPDATDSSSDEEPAV-FAPRRVKRY 60
           M VP+   R P  +P    A R+S G RIVRISMTDPDATDSSS +E A+ F  RRVKRY
Sbjct: 1   MEVPTILCREPISEPLGNFAGRSSTGSRIVRISMTDPDATDSSSSDEDALNFTRRRVKRY 60

Query: 61  VDEVTIDTAAESRGGRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSA 120
             E TI TAA+  G          VR+ ++K+K+ GNV KFRGVRRRPWGKWAAEIRDS 
Sbjct: 61  EVEFTIGTAADGGG----------VRKLKRKRKAVGNVRKFRGVRRRPWGKWAAEIRDSG 120

Query: 121 RRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDTLTNFATP-PPPFSGEE-SSPS--QSPTS 180
           RRVRLWLGTYDTAEEAAMVYDNAA+KLRGP  LTNF T  PP  +GE+ SSP+   SPTS
Sbjct: 121 RRVRLWLGTYDTAEEAAMVYDNAAVKLRGPAALTNFPTHLPPSLTGEDPSSPTNISSPTS 180

Query: 181 VLQYRSHFTESEPEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSP 240
           VL YR+ F+         CS   RAT T  + DESPVV     D P SDDIFKSIL +SP
Sbjct: 181 VL-YRTQFS--------GCSPSYRATATGCAVDESPVV-----DCPFSDDIFKSILLESP 240

Query: 241 LFVDYQTTLVPETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQGGD--DYFQDILMGS 297
           LF +YQTTL+PETPW   GGD +GS +++          AAG+ +  +  DYF++ILMGS
Sbjct: 241 LFPEYQTTLIPETPWIGGGGD-AGSSELV---------AAAGSDERAEDHDYFEEILMGS 272

BLAST of Sgr021616 vs. NCBI nr
Match: XP_023550597.1 (ethylene-responsive transcription factor CRF4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 274.6 bits (701), Expect = 9.8e-70
Identity = 167/295 (56.61%), Postives = 194/295 (65.76%), Query Frame = 0

Query: 10  MKPPEKSAVRNSPGFRIVRISMTDPDATDSSSDEEPAV-FAPRRVKRYVDEVTIDTAAES 69
           ++ P   A R + G RIVRISMTDPDATDSSS +E AV F  RRVKRYV E+TI TAA  
Sbjct: 7   LRKPGNFAGRTNVGSRIVRISMTDPDATDSSSSDEDAVNFTRRRVKRYVGEITIGTAA-G 66

Query: 70  RGGRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWLGTYDT 129
            GGR+K           KKKK  GNV KFRGVRRRPWGKWAAEIRDS RRVR+WLGTYDT
Sbjct: 67  EGGRKK-----------KKKKKTGNVRKFRGVRRRPWGKWAAEIRDSDRRVRMWLGTYDT 126

Query: 130 AEEAAMVYDNAAIKLRGPDTLTNFAT--PPPPFSGEE-SSPS--QSPTSVLQYRSHFTES 189
           AEEAA+ YD+AA+KLRGP  LTNF T  PPPPFS ++ SSP+   SPTSVL YR+H  E 
Sbjct: 127 AEEAAIAYDSAAVKLRGPTALTNFPTPPPPPPFSCDDPSSPTNLSSPTSVLHYRTHLAEC 186

Query: 190 EPEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPLFVDYQTTLVP 249
            P          RAT   G  D SPVV     DF  SDDIFKSI+ + P F++YQT+ +P
Sbjct: 187 LPSD--------RATVNGGGADVSPVV-----DFLFSDDIFKSIILEPPFFLEYQTSPIP 246

Query: 250 ETPWTDSG--GDCSGSGDILKNIHQILYSPAAGTGQGGDDYFQDILMGSDPLVVL 297
           ETPW   G  G+  G+ +  K + +             DDYF++ILMGSDPLVVL
Sbjct: 247 ETPWAGGGGWGETVGAAECNKKVEE-------------DDYFEEILMGSDPLVVL 263

BLAST of Sgr021616 vs. NCBI nr
Match: XP_022973060.1 (ethylene-responsive transcription factor CRF1-like [Cucurbita maxima])

HSP 1 Score: 269.6 bits (688), Expect = 3.1e-68
Identity = 164/294 (55.78%), Postives = 191/294 (64.97%), Query Frame = 0

Query: 10  MKPPEKSAVRNSPGFRIVRISMTDPDATDSSSDEEPAV-FAPRRVKRYVDEVTIDTAAES 69
           ++ P   A R + G RIVRISMTDPDATDSSS +E AV F  RRVKRYV E+TI TAA+ 
Sbjct: 7   LRKPGNFASRTNVGSRIVRISMTDPDATDSSSSDEDAVNFTRRRVKRYVGEITIGTAADD 66

Query: 70  RGGRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWLGTYDT 129
            GGR+K         +RKKKK  GNV KFRGVRRRPWGKWAAEIRDS RRVR+WLGTYDT
Sbjct: 67  -GGRKK---------KRKKKKQAGNVRKFRGVRRRPWGKWAAEIRDSDRRVRMWLGTYDT 126

Query: 130 AEEAAMVYDNAAIKLRGPDTLTNFAT---PPPPFSGEESSPS---QSPTSVLQYRSHFTE 189
           AEEAA+ YD+AA+KLRGP  LTNF T   PPPPFS ++ S S    SPTSVL YR+H  E
Sbjct: 127 AEEAAIAYDSAAVKLRGPTALTNFPTPPPPPPPFSCDDPSSSTNLSSPTSVLHYRTHLVE 186

Query: 190 SEPEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPLFVDYQTTLV 249
             P          R T    + D SPVV     DF  SDDIFKSI+ + P F++YQ   +
Sbjct: 187 CLPSD--------RGTVNGAAADVSPVV-----DFLFSDDIFKSIILEPPFFLEYQANPI 246

Query: 250 PETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQGGDDYFQDILMGSDPLVVL 297
           PETPW   GG   GSG+ +        +         +DYF++ILMGSDPLVVL
Sbjct: 247 PETPWAGGGG---GSGETVA-------AAECNKKVEEEDYFEEILMGSDPLVVL 267

BLAST of Sgr021616 vs. NCBI nr
Match: XP_022922366.1 (ethylene-responsive transcription factor CRF1-like [Cucurbita moschata])

HSP 1 Score: 269.2 bits (687), Expect = 4.1e-68
Identity = 163/294 (55.44%), Postives = 192/294 (65.31%), Query Frame = 0

Query: 10  MKPPEKSAVRNSPGFRIVRISMTDPDATDSSSDEEPAV-FAPRRVKRYVDEVTIDTAAES 69
           ++ P   A R + G RIVRISMTDPDATDSSS +E AV F  RRVKRYV E+TI TAA  
Sbjct: 7   LRKPGNFAGRTNVGSRIVRISMTDPDATDSSSSDEDAVNFTRRRVKRYVGEITIGTAAGE 66

Query: 70  RGGRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWLGTYDT 129
            G           R+ RKK+K  GNV KFRGVRRRPWGKWAAEIRDS RRVR+WLGTYDT
Sbjct: 67  GG-----------RKNRKKRKMAGNVRKFRGVRRRPWGKWAAEIRDSDRRVRMWLGTYDT 126

Query: 130 AEEAAMVYDNAAIKLRGPDTLTNFATPPPPFS-GEESSPS--QSPTSVLQYRSHFTESEP 189
           AEEAA+ YD+AA+KLRGP  LTNF TPPPPFS  + SSP+   SPTSVL YR+H  E  P
Sbjct: 127 AEEAAIAYDSAAVKLRGPTALTNFPTPPPPFSCNDPSSPTNLSSPTSVLHYRTHLAECLP 186

Query: 190 EQPKECSQPCRAT-GTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPLFVDYQTTLVPE 249
                     RAT    G+ D SPVV     DF  SDDIFKSI+ +   F++Y+T+ +PE
Sbjct: 187 SD--------RATVNGGGAADVSPVV-----DFLFSDDIFKSIMLEPSFFLEYETSPIPE 246

Query: 250 TPWTDSG--GDCSGSGDILKNIHQILYSPAAGTGQGGDDYFQDILMGSDPLVVL 297
           TPW   G  G+  G+ +  K + +             DDYF++ILMGSDPLVVL
Sbjct: 247 TPWAGGGGSGETVGAAECNKKVEE-------------DDYFEEILMGSDPLVVL 263

BLAST of Sgr021616 vs. ExPASy Swiss-Prot
Match: Q9SUQ2 (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.1e-37
Identity = 121/323 (37.46%), Postives = 178/323 (55.11%), Query Frame = 0

Query: 16  SAVRNSPGFRIVRISMTDPDATDSSSDEEPA-----VFAPRRVKRYVDEVTIDTAAESRG 75
           S + N+   RI+RIS+TDPDATDSSSD+E       V   RRVK++V+EV +D+ A   G
Sbjct: 26  SELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKFVNEVYLDSGAVVTG 85

Query: 76  GRRKRKADKAVRRRRKKKKSPGNVM----------KFRGVRRRPWGKWAAEIRDSARRVR 135
              + ++ K  +R  K + +   V+          KFRGVR+RPWGKWAAEIRD  +RVR
Sbjct: 86  SCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWGKWAAEIRDPLKRVR 145

Query: 136 LWLGTYDTAEEAAMVYDNAAIKLRGPDTLTNFATPPPPFSGEESSPSQSPTSVLQYRSHF 195
           LWLGTY+TAEEAAMVYDNAAI+LRGPD LTNF+  P   + E+ +P  SP    + +++ 
Sbjct: 146 LWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTT-ATEKKAPPPSPVKKKKKKNNK 205

Query: 196 TE----SEPEQPKECSQPC---------------RATGTAGSPDESPVVDYPPLDFPCSD 255
           ++    +     +  S  C                 +G + SP  + VV   P     S+
Sbjct: 206 SKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGISSSPVAAVVVKEEPSMTTVSE 265

Query: 256 ---DIFKSILSDSPLFVDYQTTLVPETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQG 297
              D    + SD  +F D+++++VP+    D  G+   + D+  +++   +    G+G  
Sbjct: 266 TFSDFSAPLFSDDDVF-DFRSSVVPDYLGGDLFGEDLFTADMCTDMN---FGFDFGSGLS 325

BLAST of Sgr021616 vs. ExPASy Swiss-Prot
Match: Q9SUE3 (Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN=CRF4 PE=1 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 1.6e-35
Identity = 111/270 (41.11%), Postives = 145/270 (53.70%), Query Frame = 0

Query: 15  KSAVRNSPGFRIVRISMTDPDATDSSSDEEPAVFAPRRVKRYVDEVTIDTAAESRG---- 74
           K++VR+S   R+VR+SMTD DATDSSSDEE  +F  RRVKR ++E+ ++ ++ S G    
Sbjct: 33  KTSVRDSA--RLVRVSMTDRDATDSSSDEEEFLFPRRRVKRLINEIRVEPSSSSTGDVSA 92

Query: 75  ----GRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWLGTY 134
                R++   D  V   +K   S  N  K+RGVR+RPWGKWAAEIRD  +R R+WLGT+
Sbjct: 93  SPTKDRKRINVDSTV---QKPSVSGQNQKKYRGVRQRPWGKWAAEIRDPEQRRRIWLGTF 152

Query: 135 DTAEEAAMVYDNAAIKLRGPDTLTNFATPPPP-------------------FSGEESSPS 194
            TAEEAA+VYDNAAIKLRGPD LTNF   P P                    S ++S   
Sbjct: 153 ATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQEPESNMSVSISESMDDSQHL 212

Query: 195 QSPTSVLQYRSHFTES--------------EPEQPKECSQPCRATGTAGSPDESPVVDYP 244
            SPTSVL Y+++ +E               EPEQ  E        G   + D+S      
Sbjct: 213 SSPTSVLNYQTYVSEEPIDSLIKPVKQEFLEPEQ--EPISWHLGEGNTNTNDDS-----F 272

BLAST of Sgr021616 vs. ExPASy Swiss-Prot
Match: O82503 (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.9e-32
Identity = 118/304 (38.82%), Postives = 154/304 (50.66%), Query Frame = 0

Query: 25  RIVRISMTDPDATDSSSDEEPAV------FAPRRVKRYVDEVTIDTAAESRGGRRKRKAD 84
           RI+RIS+TDP ATDSSSDEE  V         RRVK+YV EV +D+    +        +
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDK--------E 70

Query: 85  KAVRRRRKKKKSPGNVM------KFRGVRRRPWGKWAAEIRDSARRVRLWLGTYDTAEEA 144
           K ++++RKK+     V+      KFRGVR+RPWGKWAAEIRD +RRVR+WLGT+DTAEEA
Sbjct: 71  KPMKKKRKKRVVTVPVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEA 130

Query: 145 AMVYDNAAIKLRGPDTLTNFATPPPPFSGEESSPS---------------QSPTSVLQYR 204
           A+VYDNAAI+LRGP+   NF  PP   + EE+S                 +SP SVL+  
Sbjct: 131 AIVYDNAAIQLRGPNAELNFPPPPVTENVEEASTEVKGVSDFIIGGGECLRSPVSVLE-- 190

Query: 205 SHFTESEPEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPLFVDY 264
           S F+       +E           G      VV   P  F  SD         +PLF D 
Sbjct: 191 SPFSGESTAVKEE---------FVGVSTAEIVVKKEP-SFNGSD-------FSAPLFSDD 250

Query: 265 QTTLVPETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQGG---DDYFQDI--LMGSDP 297
                  +     GGD  G            +   +G+G      +D+FQDI  L GSDP
Sbjct: 251 DVFGFSTSMSESFGGDLFGDNLFADMSFGSGFGFGSGSGFSSWHVEDHFQDIGDLFGSDP 287

BLAST of Sgr021616 vs. ExPASy Swiss-Prot
Match: Q9FK12 (Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN=CRF3 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.4e-31
Identity = 112/279 (40.14%), Postives = 149/279 (53.41%), Query Frame = 0

Query: 11  KPPEKSAVRNSPGFRIVRISM--TDPDATDSSS--DEEPAVFAPRRVKRYVDEVTID--- 70
           K  +KS+ + S G  + ++S+  TDPDATDSSS  DEE  +F  RRVKR+V+E+T++   
Sbjct: 21  KYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRRRVKRFVNEITVEPSC 80

Query: 71  ----TAAESRGGRR------KRKADKAVRRRRKKKKS-PGNVMKFRGVRRRPWGKWAAEI 130
               T    +  +R      + ++  + R+R   K S  G + KFRGVR+RPWGKWAAEI
Sbjct: 81  NNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSGQIKKFRGVRQRPWGKWAAEI 140

Query: 131 RDSARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDTLTNFATPP---------------- 190
           RD  +R R+WLGT++TAEEAA+VYDNAAI+LRGPD LTNF+ PP                
Sbjct: 141 RDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPPQEEEEEEEPEPVIEEK 200

Query: 191 --------PPFSGEESSPS-----QSPTSVLQYRSH--------FTESEPEQPKECSQPC 235
                   P  S  ES+        SPTSVL +RS         F  ++PE P   + P 
Sbjct: 201 PVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQVQQPFKSAKPE-PGVSNAPW 260

BLAST of Sgr021616 vs. ExPASy Swiss-Prot
Match: O04682 (Pathogenesis-related genes transcriptional activator PTI6 OS=Solanum lycopersicum OX=4081 GN=PTI6 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.5e-28
Identity = 75/141 (53.19%), Postives = 93/141 (65.96%), Query Frame = 0

Query: 14  EKSAVRNSPGFRIVRISMTDPDATDSSSDEEPAVFAPRRVKRYVDEVTIDTAAESRGGRR 73
           E +   NS   R+VRI +TD DATDSS DE       RRVKR+V E+ +  + +S G R+
Sbjct: 25  EHNEKSNSELQRVVRIILTDADATDSSDDEGRNTV--RRVKRHVTEINLMPSTKSIGDRK 84

Query: 74  KRKA--DKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWLGTYDTAEE 133
           +R    D  V RR+          KFRGVR+RPWG+WAAEIRD  R  R+WLGTYDT EE
Sbjct: 85  RRSVSPDSDVTRRK----------KFRGVRQRPWGRWAAEIRDPTRGKRVWLGTYDTPEE 144

Query: 134 AAMVYDNAAIKLRGPDTLTNF 153
           AA+VYD AA+KL+GPD +TNF
Sbjct: 145 AAVVYDKAAVKLKGPDAVTNF 153

BLAST of Sgr021616 vs. ExPASy TrEMBL
Match: A0A6J1DVM1 (ethylene-responsive transcription factor CRF2-like OS=Momordica charantia OX=3673 GN=LOC111023926 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 3.0e-80
Identity = 193/305 (63.28%), Postives = 216/305 (70.82%), Query Frame = 0

Query: 1   MNVPS---RNPTMKPPEKSAVRNSPGFRIVRISMTDPDATDSSSDEEPA-VFAPRRVKRY 60
           M+VP+   R PT KPPE     NS G RIVRIS+TDPDATDSSSD+EPA +   RR+K Y
Sbjct: 1   MDVPTNLRRKPTTKPPE-----NSAGSRIVRISITDPDATDSSSDDEPAMLLTRRRLKCY 60

Query: 61  VDEVTIDTAAESRGGRRKRKADKAVRRRRK-KKKSPGNVMKFRGVRRRPWGKWAAEIRDS 120
           VDEVTI+T AE+ GGRRKR A+  VRRRR+ KKKSPGNV KF GVRRRPWGKW     DS
Sbjct: 61  VDEVTIET-AETGGGRRKRTANGLVRRRRRMKKKSPGNVGKFLGVRRRPWGKW-----DS 120

Query: 121 ARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDTLTNFAT--PPPPFSGEESSPSQSPTSV 180
             R+RLW GTYDTAE+      NAA+K RGP  LTNF T  PPPPF GE+S  S SPTSV
Sbjct: 121 GCRIRLWFGTYDTAED-----KNAAMKFRGPAVLTNFPTPPPPPPFPGEDS--SSSPTSV 180

Query: 181 LQYRSHFTESEPEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPL 240
           L Y +HFTES+PE P     PCR T T G   ESPVV     DFPCSDDIFKSIL +SPL
Sbjct: 181 LHYGTHFTESKPEPP-----PCRTTATFGVVAESPVV-----DFPCSDDIFKSILFESPL 240

Query: 241 FVDYQTTLVPETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQGGD--DYFQDILMGSD 297
           F DYQT LVPETPWTD       SG       + LYS AAG+GQGG+  D+F++ILMGSD
Sbjct: 241 FFDYQTNLVPETPWTD-----GSSG-------EFLYSAAAGSGQGGEEHDFFEEILMGSD 265

BLAST of Sgr021616 vs. ExPASy TrEMBL
Match: A0A6J1IAE5 (ethylene-responsive transcription factor CRF1-like OS=Cucurbita maxima OX=3661 GN=LOC111471560 PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.5e-68
Identity = 164/294 (55.78%), Postives = 191/294 (64.97%), Query Frame = 0

Query: 10  MKPPEKSAVRNSPGFRIVRISMTDPDATDSSSDEEPAV-FAPRRVKRYVDEVTIDTAAES 69
           ++ P   A R + G RIVRISMTDPDATDSSS +E AV F  RRVKRYV E+TI TAA+ 
Sbjct: 7   LRKPGNFASRTNVGSRIVRISMTDPDATDSSSSDEDAVNFTRRRVKRYVGEITIGTAADD 66

Query: 70  RGGRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWLGTYDT 129
            GGR+K         +RKKKK  GNV KFRGVRRRPWGKWAAEIRDS RRVR+WLGTYDT
Sbjct: 67  -GGRKK---------KRKKKKQAGNVRKFRGVRRRPWGKWAAEIRDSDRRVRMWLGTYDT 126

Query: 130 AEEAAMVYDNAAIKLRGPDTLTNFAT---PPPPFSGEESSPS---QSPTSVLQYRSHFTE 189
           AEEAA+ YD+AA+KLRGP  LTNF T   PPPPFS ++ S S    SPTSVL YR+H  E
Sbjct: 127 AEEAAIAYDSAAVKLRGPTALTNFPTPPPPPPPFSCDDPSSSTNLSSPTSVLHYRTHLVE 186

Query: 190 SEPEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPLFVDYQTTLV 249
             P          R T    + D SPVV     DF  SDDIFKSI+ + P F++YQ   +
Sbjct: 187 CLPSD--------RGTVNGAAADVSPVV-----DFLFSDDIFKSIILEPPFFLEYQANPI 246

Query: 250 PETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQGGDDYFQDILMGSDPLVVL 297
           PETPW   GG   GSG+ +        +         +DYF++ILMGSDPLVVL
Sbjct: 247 PETPWAGGGG---GSGETVA-------AAECNKKVEEEDYFEEILMGSDPLVVL 267

BLAST of Sgr021616 vs. ExPASy TrEMBL
Match: A0A6J1E3Y2 (ethylene-responsive transcription factor CRF1-like OS=Cucurbita moschata OX=3662 GN=LOC111430377 PE=4 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 2.0e-68
Identity = 163/294 (55.44%), Postives = 192/294 (65.31%), Query Frame = 0

Query: 10  MKPPEKSAVRNSPGFRIVRISMTDPDATDSSSDEEPAV-FAPRRVKRYVDEVTIDTAAES 69
           ++ P   A R + G RIVRISMTDPDATDSSS +E AV F  RRVKRYV E+TI TAA  
Sbjct: 7   LRKPGNFAGRTNVGSRIVRISMTDPDATDSSSSDEDAVNFTRRRVKRYVGEITIGTAAGE 66

Query: 70  RGGRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWLGTYDT 129
            G           R+ RKK+K  GNV KFRGVRRRPWGKWAAEIRDS RRVR+WLGTYDT
Sbjct: 67  GG-----------RKNRKKRKMAGNVRKFRGVRRRPWGKWAAEIRDSDRRVRMWLGTYDT 126

Query: 130 AEEAAMVYDNAAIKLRGPDTLTNFATPPPPFS-GEESSPS--QSPTSVLQYRSHFTESEP 189
           AEEAA+ YD+AA+KLRGP  LTNF TPPPPFS  + SSP+   SPTSVL YR+H  E  P
Sbjct: 127 AEEAAIAYDSAAVKLRGPTALTNFPTPPPPFSCNDPSSPTNLSSPTSVLHYRTHLAECLP 186

Query: 190 EQPKECSQPCRAT-GTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPLFVDYQTTLVPE 249
                     RAT    G+ D SPVV     DF  SDDIFKSI+ +   F++Y+T+ +PE
Sbjct: 187 SD--------RATVNGGGAADVSPVV-----DFLFSDDIFKSIMLEPSFFLEYETSPIPE 246

Query: 250 TPWTDSG--GDCSGSGDILKNIHQILYSPAAGTGQGGDDYFQDILMGSDPLVVL 297
           TPW   G  G+  G+ +  K + +             DDYF++ILMGSDPLVVL
Sbjct: 247 TPWAGGGGSGETVGAAECNKKVEE-------------DDYFEEILMGSDPLVVL 263

BLAST of Sgr021616 vs. ExPASy TrEMBL
Match: A0A0A0KJI1 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139630 PE=4 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 4.6e-65
Identity = 170/294 (57.82%), Postives = 199/294 (67.69%), Query Frame = 0

Query: 11  KPPEKSAVRNSPGFRIVRISMTDPDATD-SSSDEEPAVFAPRRVKRYVDEVTIDTAAESR 70
           KP  +   R+S G RIVRISM DPDATD SSSDE+   +  RRVKRY  E +I  A +  
Sbjct: 10  KPISEPHGRSSTGSRIVRISMADPDATDSSSSDEDTLNYTSRRVKRYEIEFSIGMAGDGD 69

Query: 71  G-GRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWLGTYDT 130
           G G RK K       R++K+K+ GNV KFRGVRRRPWGKWAAEIRDS  RVRLWLGTYDT
Sbjct: 70  GCGDRKLK-------RKRKRKAVGNVSKFRGVRRRPWGKWAAEIRDSGSRVRLWLGTYDT 129

Query: 131 AEEAAMVYDNAAIKLRGPDTLTNFAT-PPPPFSGEE-SSPS--QSPTSVLQYRSHFTESE 190
           AEEAAMVYD+AA+KLRGP  LTNF T PPPP +G+E SSP+   SPTSVL +R+HFT   
Sbjct: 130 AEEAAMVYDSAALKLRGPAALTNFPTHPPPPSTGQEPSSPTNISSPTSVL-HRTHFT--- 189

Query: 191 PEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPLFVDYQTTLVPE 250
                ECS   RA        ESPVVDY P     SDDIFKSIL +SPLF +YQ+TL+ E
Sbjct: 190 -----ECSSSYRAV------IESPVVDYCPF----SDDIFKSILLESPLFPEYQSTLITE 249

Query: 251 TPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQGGDDY--FQDILMGSDPLVVL 297
            PW D+GG  + SGD        L   AAG+ +  +D+  F++ILMGSDPLVVL
Sbjct: 250 APWIDAGGG-ARSGD--------LVVAAAGSDERVEDHDCFEEILMGSDPLVVL 268

BLAST of Sgr021616 vs. ExPASy TrEMBL
Match: A0A5D3C6F5 (Ethylene-responsive transcription factor CRF1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00650 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.7e-64
Identity = 165/299 (55.18%), Postives = 196/299 (65.55%), Query Frame = 0

Query: 6   RNPTMKPPEKSAVRNSPGFRIVRISMTDPDATDSSSDEEPAVFAP-RRVKRYVDEVTIDT 65
           R P  +PP     R+S G RIVRISMTDPDATDSSS +E  +  P RRVKRY  E+++ T
Sbjct: 9   RKPISEPPGNFHGRSSTGSRIVRISMTDPDATDSSSSDEDTLNYPRRRVKRYEIELSVGT 68

Query: 66  AAESRG-GRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWL 125
           A +  G G RK K       R++K+K+ GNV KFRGVRRRPWGKWAAEIRD+ RRVRLWL
Sbjct: 69  AGDGGGCGARKLK-------RKRKRKAVGNVSKFRGVRRRPWGKWAAEIRDTGRRVRLWL 128

Query: 126 GTYDTAEEAAMVYDNAAIKLRGPDTLTNFA--TPPPPFSGEESSPS--QSPTSVLQYRSH 185
           GTYDTAEEAAMVYD+AA+KLRGP  LTNF   TPPP    E SSP+   SPTSVL YR+ 
Sbjct: 129 GTYDTAEEAAMVYDSAALKLRGPAALTNFPTHTPPPSTGDEPSSPTNISSPTSVL-YRTQ 188

Query: 186 FTESEPEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPLFVDYQT 245
           FT        ECS   RA           V D P +D P SDDIFKS L +SPLF +YQ+
Sbjct: 189 FT--------ECSSSYRA-----------VNDSPVVDCPFSDDIFKSSLLESPLFPEYQS 248

Query: 246 TLVPETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQGGD--DYFQDILMGSDPLVVL 297
           TL+ E PW D+ G  + S D++          AAG+ +  +  DYF++ILMGSDPLVVL
Sbjct: 249 TLITEAPWIDADGG-ARSSDLV---------AAAGSDERVEDHDYFEEILMGSDPLVVL 270

BLAST of Sgr021616 vs. TAIR 10
Match: AT4G23750.1 (cytokinin response factor 2 )

HSP 1 Score: 156.4 bits (394), Expect = 3.6e-38
Identity = 121/323 (37.46%), Postives = 178/323 (55.11%), Query Frame = 0

Query: 16  SAVRNSPGFRIVRISMTDPDATDSSSDEEPA-----VFAPRRVKRYVDEVTIDTAAESRG 75
           S + N+   RI+RIS+TDPDATDSSSD+E       V   RRVK++V+EV +D+ A   G
Sbjct: 26  SELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKFVNEVYLDSGAVVTG 85

Query: 76  GRRKRKADKAVRRRRKKKKSPGNVM----------KFRGVRRRPWGKWAAEIRDSARRVR 135
              + ++ K  +R  K + +   V+          KFRGVR+RPWGKWAAEIRD  +RVR
Sbjct: 86  SCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWGKWAAEIRDPLKRVR 145

Query: 136 LWLGTYDTAEEAAMVYDNAAIKLRGPDTLTNFATPPPPFSGEESSPSQSPTSVLQYRSHF 195
           LWLGTY+TAEEAAMVYDNAAI+LRGPD LTNF+  P   + E+ +P  SP    + +++ 
Sbjct: 146 LWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTT-ATEKKAPPPSPVKKKKKKNNK 205

Query: 196 TE----SEPEQPKECSQPC---------------RATGTAGSPDESPVVDYPPLDFPCSD 255
           ++    +     +  S  C                 +G + SP  + VV   P     S+
Sbjct: 206 SKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGISSSPVAAVVVKEEPSMTTVSE 265

Query: 256 ---DIFKSILSDSPLFVDYQTTLVPETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQG 297
              D    + SD  +F D+++++VP+    D  G+   + D+  +++   +    G+G  
Sbjct: 266 TFSDFSAPLFSDDDVF-DFRSSVVPDYLGGDLFGEDLFTADMCTDMN---FGFDFGSGLS 325

BLAST of Sgr021616 vs. TAIR 10
Match: AT4G23750.2 (cytokinin response factor 2 )

HSP 1 Score: 156.4 bits (394), Expect = 3.6e-38
Identity = 121/323 (37.46%), Postives = 178/323 (55.11%), Query Frame = 0

Query: 16  SAVRNSPGFRIVRISMTDPDATDSSSDEEPA-----VFAPRRVKRYVDEVTIDTAAESRG 75
           S + N+   RI+RIS+TDPDATDSSSD+E       V   RRVK++V+EV +D+ A   G
Sbjct: 26  SELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKFVNEVYLDSGAVVTG 85

Query: 76  GRRKRKADKAVRRRRKKKKSPGNVM----------KFRGVRRRPWGKWAAEIRDSARRVR 135
              + ++ K  +R  K + +   V+          KFRGVR+RPWGKWAAEIRD  +RVR
Sbjct: 86  SCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWGKWAAEIRDPLKRVR 145

Query: 136 LWLGTYDTAEEAAMVYDNAAIKLRGPDTLTNFATPPPPFSGEESSPSQSPTSVLQYRSHF 195
           LWLGTY+TAEEAAMVYDNAAI+LRGPD LTNF+  P   + E+ +P  SP    + +++ 
Sbjct: 146 LWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTT-ATEKKAPPPSPVKKKKKKNNK 205

Query: 196 TE----SEPEQPKECSQPC---------------RATGTAGSPDESPVVDYPPLDFPCSD 255
           ++    +     +  S  C                 +G + SP  + VV   P     S+
Sbjct: 206 SKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGISSSPVAAVVVKEEPSMTTVSE 265

Query: 256 ---DIFKSILSDSPLFVDYQTTLVPETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQG 297
              D    + SD  +F D+++++VP+    D  G+   + D+  +++   +    G+G  
Sbjct: 266 TFSDFSAPLFSDDDVF-DFRSSVVPDYLGGDLFGEDLFTADMCTDMN---FGFDFGSGLS 325

BLAST of Sgr021616 vs. TAIR 10
Match: AT4G27950.1 (cytokinin response factor 4 )

HSP 1 Score: 151.4 bits (381), Expect = 1.2e-36
Identity = 111/270 (41.11%), Postives = 145/270 (53.70%), Query Frame = 0

Query: 15  KSAVRNSPGFRIVRISMTDPDATDSSSDEEPAVFAPRRVKRYVDEVTIDTAAESRG---- 74
           K++VR+S   R+VR+SMTD DATDSSSDEE  +F  RRVKR ++E+ ++ ++ S G    
Sbjct: 33  KTSVRDSA--RLVRVSMTDRDATDSSSDEEEFLFPRRRVKRLINEIRVEPSSSSTGDVSA 92

Query: 75  ----GRRKRKADKAVRRRRKKKKSPGNVMKFRGVRRRPWGKWAAEIRDSARRVRLWLGTY 134
                R++   D  V   +K   S  N  K+RGVR+RPWGKWAAEIRD  +R R+WLGT+
Sbjct: 93  SPTKDRKRINVDSTV---QKPSVSGQNQKKYRGVRQRPWGKWAAEIRDPEQRRRIWLGTF 152

Query: 135 DTAEEAAMVYDNAAIKLRGPDTLTNFATPPPP-------------------FSGEESSPS 194
            TAEEAA+VYDNAAIKLRGPD LTNF   P P                    S ++S   
Sbjct: 153 ATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQEPESNMSVSISESMDDSQHL 212

Query: 195 QSPTSVLQYRSHFTES--------------EPEQPKECSQPCRATGTAGSPDESPVVDYP 244
            SPTSVL Y+++ +E               EPEQ  E        G   + D+S      
Sbjct: 213 SSPTSVLNYQTYVSEEPIDSLIKPVKQEFLEPEQ--EPISWHLGEGNTNTNDDS-----F 272

BLAST of Sgr021616 vs. TAIR 10
Match: AT4G11140.1 (cytokinin response factor 1 )

HSP 1 Score: 140.6 bits (353), Expect = 2.1e-33
Identity = 118/304 (38.82%), Postives = 154/304 (50.66%), Query Frame = 0

Query: 25  RIVRISMTDPDATDSSSDEEPAV------FAPRRVKRYVDEVTIDTAAESRGGRRKRKAD 84
           RI+RIS+TDP ATDSSSDEE  V         RRVK+YV EV +D+    +        +
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDK--------E 70

Query: 85  KAVRRRRKKKKSPGNVM------KFRGVRRRPWGKWAAEIRDSARRVRLWLGTYDTAEEA 144
           K ++++RKK+     V+      KFRGVR+RPWGKWAAEIRD +RRVR+WLGT+DTAEEA
Sbjct: 71  KPMKKKRKKRVVTVPVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEA 130

Query: 145 AMVYDNAAIKLRGPDTLTNFATPPPPFSGEESSPS---------------QSPTSVLQYR 204
           A+VYDNAAI+LRGP+   NF  PP   + EE+S                 +SP SVL+  
Sbjct: 131 AIVYDNAAIQLRGPNAELNFPPPPVTENVEEASTEVKGVSDFIIGGGECLRSPVSVLE-- 190

Query: 205 SHFTESEPEQPKECSQPCRATGTAGSPDESPVVDYPPLDFPCSDDIFKSILSDSPLFVDY 264
           S F+       +E           G      VV   P  F  SD         +PLF D 
Sbjct: 191 SPFSGESTAVKEE---------FVGVSTAEIVVKKEP-SFNGSD-------FSAPLFSDD 250

Query: 265 QTTLVPETPWTDSGGDCSGSGDILKNIHQILYSPAAGTGQGG---DDYFQDI--LMGSDP 297
                  +     GGD  G            +   +G+G      +D+FQDI  L GSDP
Sbjct: 251 DVFGFSTSMSESFGGDLFGDNLFADMSFGSGFGFGSGSGFSSWHVEDHFQDIGDLFGSDP 287

BLAST of Sgr021616 vs. TAIR 10
Match: AT5G53290.1 (cytokinin response factor 3 )

HSP 1 Score: 138.3 bits (347), Expect = 1.0e-32
Identity = 112/279 (40.14%), Postives = 149/279 (53.41%), Query Frame = 0

Query: 11  KPPEKSAVRNSPGFRIVRISM--TDPDATDSSS--DEEPAVFAPRRVKRYVDEVTID--- 70
           K  +KS+ + S G  + ++S+  TDPDATDSSS  DEE  +F  RRVKR+V+E+T++   
Sbjct: 21  KYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRRRVKRFVNEITVEPSC 80

Query: 71  ----TAAESRGGRR------KRKADKAVRRRRKKKKS-PGNVMKFRGVRRRPWGKWAAEI 130
               T    +  +R      + ++  + R+R   K S  G + KFRGVR+RPWGKWAAEI
Sbjct: 81  NNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSGQIKKFRGVRQRPWGKWAAEI 140

Query: 131 RDSARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDTLTNFATPP---------------- 190
           RD  +R R+WLGT++TAEEAA+VYDNAAI+LRGPD LTNF+ PP                
Sbjct: 141 RDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPPQEEEEEEEPEPVIEEK 200

Query: 191 --------PPFSGEESSPS-----QSPTSVLQYRSH--------FTESEPEQPKECSQPC 235
                   P  S  ES+        SPTSVL +RS         F  ++PE P   + P 
Sbjct: 201 PVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQVQQPFKSAKPE-PGVSNAPW 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022157129.16.1e-8063.28ethylene-responsive transcription factor CRF2-like [Momordica charantia][more]
XP_038875714.18.0e-7258.50ethylene-responsive transcription factor CRF1 [Benincasa hispida][more]
XP_023550597.19.8e-7056.61ethylene-responsive transcription factor CRF4-like [Cucurbita pepo subsp. pepo][more]
XP_022973060.13.1e-6855.78ethylene-responsive transcription factor CRF1-like [Cucurbita maxima][more]
XP_022922366.14.1e-6855.44ethylene-responsive transcription factor CRF1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SUQ25.1e-3737.46Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SUE31.6e-3541.11Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN... [more]
O825032.9e-3238.82Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FK121.4e-3140.14Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN... [more]
O046821.5e-2853.19Pathogenesis-related genes transcriptional activator PTI6 OS=Solanum lycopersicu... [more]
Match NameE-valueIdentityDescription
A0A6J1DVM13.0e-8063.28ethylene-responsive transcription factor CRF2-like OS=Momordica charantia OX=367... [more]
A0A6J1IAE51.5e-6855.78ethylene-responsive transcription factor CRF1-like OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1E3Y22.0e-6855.44ethylene-responsive transcription factor CRF1-like OS=Cucurbita moschata OX=3662... [more]
A0A0A0KJI14.6e-6557.82AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139630 PE=... [more]
A0A5D3C6F51.7e-6455.18Ethylene-responsive transcription factor CRF1-like OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
AT4G23750.13.6e-3837.46cytokinin response factor 2 [more]
AT4G23750.23.6e-3837.46cytokinin response factor 2 [more]
AT4G27950.11.2e-3641.11cytokinin response factor 4 [more]
AT4G11140.12.1e-3338.82cytokinin response factor 1 [more]
AT5G53290.11.0e-3240.14cytokinin response factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 119..135
score: 61.5
coord: 97..108
score: 69.39
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 96..159
e-value: 2.3E-36
score: 136.8
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 96..145
e-value: 2.1E-14
score: 53.5
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 96..153
score: 23.208761
IPR001471AP2/ERF domainCDDcd00018AP2coord: 96..153
e-value: 3.65266E-32
score: 111.595
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 95..154
e-value: 9.0E-26
score: 91.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..48
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 64..98
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 154..181
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..98
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 147..209
NoneNo IPR availablePANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 14..287
NoneNo IPR availablePANTHERPTHR31194:SF77ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF1-LIKEcoord: 14..287
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 96..153

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr021616.1Sgr021616.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity