Homology
BLAST of Sgr021310 vs. NCBI nr
Match:
AEM42992.1 (cytochrome P450 [Siraitia grosvenorii])
HSP 1 Score: 997.3 bits (2577), Expect = 4.8e-287
Identity = 501/502 (99.80%), Postives = 501/502 (99.80%), Query Frame = 0
Query: 1 MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP
Sbjct: 1 MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
Query: 61 IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR
Sbjct: 61 IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM
Sbjct: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL
Sbjct: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
Query: 241 TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD 300
TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD
Sbjct: 241 TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD 300
Query: 301 TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP 360
TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP
Sbjct: 301 TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP 360
Query: 361 AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ 420
AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ
Sbjct: 361 AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ 420
Query: 421 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP 480
SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP
Sbjct: 421 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP 480
Query: 481 LEAMCKARPAMDKLLLNALDKI 503
LEAMCKARPAM KLLLNALDKI
Sbjct: 481 LEAMCKARPAMYKLLLNALDKI 502
BLAST of Sgr021310 vs. NCBI nr
Match:
XP_022944057.1 (cytochrome P450 81E8-like [Cucurbita moschata] >KAG7011030.1 Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 770.4 bits (1988), Expect = 9.6e-219
Identity = 375/502 (74.70%), Postives = 437/502 (87.05%), Query Frame = 0
Query: 1 MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
MDF LLLLL L L Q TRRRNLPPSPPS PIIGHLHLL+RPIHRNFH IAA+YGP
Sbjct: 1 MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
Query: 61 IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
IF+LRFGSRLA+IVSSL +AEECFTKND++FANRPRLLI K+LGYN TT++T+P+GDHWR
Sbjct: 61 IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
NLRRL ++EIFSTARLN++LSIRKDEI+RLL+KLHS S F KVELK+MFSEL+FN +M
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVM 180
Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
R+V GKRY+G+EVS+E EAREFR LM+E S HGGAS+WVDFMPILKWIG G +EKSL +L
Sbjct: 181 RVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKL 240
Query: 241 TKRTDKFMQALIEERRNKKVL--ERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300
TKR DKFMQ L+E+RRN+ L E++++LLDRLLELQASEPEYY+D+IIKG++LVLLRAG
Sbjct: 241 TKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAG 300
Query: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360
D+++VT+ WAM QLLNNPE+++KAKAE+DTKIG+DR +DE DL NL YLQAI+SET RL
Sbjct: 301 ADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRL 360
Query: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420
HPAAPMLL+HYSS DCTVAGYD+PRGT LLVNAWAIHRDPKLWDDP SFRPERFLG NE
Sbjct: 361 HPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNE 420
Query: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480
L KLI FG+GRR+CPG+TMALR +GL+LGLLIQCY+WK G EKVDM EGGGITI K
Sbjct: 421 LLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKV 480
Query: 481 KPLEAMCKARPAMDKLLLNALD 501
+PLE MC+A P MDK+L N LD
Sbjct: 481 QPLETMCRALPIMDKVLSNGLD 499
BLAST of Sgr021310 vs. NCBI nr
Match:
XP_022985607.1 (cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E8-like [Cucurbita maxima])
HSP 1 Score: 767.3 bits (1980), Expect = 8.1e-218
Identity = 373/502 (74.30%), Postives = 439/502 (87.45%), Query Frame = 0
Query: 1 MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
MDF S FLLLLL L L Q TRRRNLPPSPPS+PIIGHLHLL+RPIH+NFH IAA+YGP
Sbjct: 1 MDFLSVFLLLLLVFLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAKYGP 60
Query: 61 IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
IF+LRFGSRLA+IVSSL +AEECFTKND++FANRPRLLI K+LGYN TT++T+PYGD WR
Sbjct: 61 IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDLWR 120
Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
NLRRL ++E+FSTARLN++LSIRKDEI+RLL+KLHS S F KVELK+MFSEL+FN +M
Sbjct: 121 NLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVM 180
Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
R+V GKRY+G+EVS+E EAREFR LM+EIS HGGAS+W++FMPILKWIG G +EKSL +L
Sbjct: 181 RVVTGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPILKWIGFGEYEKSLAKL 240
Query: 241 TKRTDKFMQALIEERRNKK--VLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300
TKR DKFMQ L+E RRN+ +E++++LLDRLLELQASEPEYY+D+IIKG+VLVLLRAG
Sbjct: 241 TKRADKFMQKLVEYRRNQTGFEIEKQSTLLDRLLELQASEPEYYSDEIIKGVVLVLLRAG 300
Query: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360
D+++VT+ WAM QLLNNPE+++KAKAE+DTKIG+DR VDE DL NL YLQAI+ ET RL
Sbjct: 301 VDSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETFRL 360
Query: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420
HPAAPMLL+HYSS DCTVAGYDIPRGT L+VNAWAIHRDPKLWDDP SFRPERFLGA NE
Sbjct: 361 HPAAPMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAGNE 420
Query: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480
L S KL+ FG+GRR+CPG+TMA R +GL+LGLLIQCY+WK G +KVDM EGGGITI K
Sbjct: 421 LLSNKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQKV 480
Query: 481 KPLEAMCKARPAMDKLLLNALD 501
+PLE MC+A P MDK+L N LD
Sbjct: 481 QPLETMCRALPIMDKVLSNGLD 499
BLAST of Sgr021310 vs. NCBI nr
Match:
XP_022149457.1 (cytochrome P450 81E8-like isoform X2 [Momordica charantia])
HSP 1 Score: 765.8 bits (1976), Expect = 2.4e-217
Identity = 389/512 (75.98%), Postives = 436/512 (85.16%), Query Frame = 0
Query: 1 MDFFSAFLLLLL---TVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAE 60
MD S F LLL ++L QIR RRNLPPSPPS+PIIGHL LL+RP+HRNF KIAAE
Sbjct: 1 MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
Query: 61 YGPIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGD 120
YGPIFSLRFGSRLAV+VSSLD AEEC TKND++FANRPRL+I KHLGYN TTMA +PYG
Sbjct: 61 YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
Query: 121 HWRNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLH----SGSSGEFTKVELKTMFSE 180
HWRNLRRL A E+FSTARL SSLSIRK+EI+R+LLKL SGS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
Query: 181 LAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIG-GGG 240
+ FN +MR+VAGKRYYG+EVSDE EAREF LMEEIS HGGAS+WVDFMPIL+WIG GG
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
Query: 241 FEKSLVRLTKRTDKFMQALIEE-RRNKK--VLERKN--SLLDRLLELQASEPEYYTDQII 300
+E++L +L KRTDKFM+AL+EE R NKK +ERKN SLLDRLLELQ SEP+YYTDQI+
Sbjct: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
Query: 301 KGLVLVLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSY 360
KGLVLVLLRAGTD+++VT+NWAM QLLNNPE+L KAKAELDTK+GQDR VDE DL NL Y
Sbjct: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360
Query: 361 LQAIVSETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSF 420
LQAIV ETLRLHP APML++HYSS+DCTVAGY IP T LLVNAWAIHRDPKLWDDPTSF
Sbjct: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420
Query: 421 RPERFLG-AANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVD 480
RPERFLG AANEL S KLIAFGLGRR+CPG TM RFVGLTL L+IQCY+WKK G+E +D
Sbjct: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480
Query: 481 MGEGGGITIHKAKPLEAMCKARPAMDKLLLNA 499
MGEGGGITI KAKPLE MCKARP MDK+ + A
Sbjct: 481 MGEGGGITILKAKPLEVMCKARPVMDKVHVKA 512
BLAST of Sgr021310 vs. NCBI nr
Match:
XP_023512643.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 765.8 bits (1976), Expect = 2.4e-217
Identity = 373/502 (74.30%), Postives = 436/502 (86.85%), Query Frame = 0
Query: 1 MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
MDF S LLLLL L L Q T RRNLPPSPPS+PIIGHLHLL+RPIHRNFH IAA+YGP
Sbjct: 1 MDFLSVLLLLLLVFLFLQQFITCRRNLPPSPPSVPIIGHLHLLQRPIHRNFHHIAAKYGP 60
Query: 61 IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
IF+LRFGSRLA+IVSSL +AEECFTKND++FANRPRLLI K+LGYN TT++T+PYGDHWR
Sbjct: 61 IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDHWR 120
Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
NLRRL ++EIFSTARLN++LSIRKDEI+RLL+KLHS S F +VELK+MFSEL+FN +M
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FARVELKSMFSELSFNVVM 180
Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
R+V GKRY+G+EVS+E EAREFR LM+E S HGGAS+W+DFMPILKWIG G +EKSL +L
Sbjct: 181 RVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWIDFMPILKWIGFGEYEKSLAKL 240
Query: 241 TKRTDKFMQALIEERRNKKVL--ERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300
TKR DKFMQ L+E+RRN+ L E++++LLDRLLELQASEPEYY+D+IIKG++LVLLRAG
Sbjct: 241 TKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAG 300
Query: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360
D+++VT+ WAM QLLNNPE+++KAKAE+DTKIG+DR VDE DL NL YLQAI+SET RL
Sbjct: 301 ADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDEADLTNLHYLQAIISETFRL 360
Query: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420
HPAAPMLL+HYSS DCTVAGYDIPRGT LLVNAWAIHRDPKLWDDP FRPERFLG NE
Sbjct: 361 HPAAPMLLTHYSSDDCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPMRFRPERFLGEGNE 420
Query: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480
L S KLI FG+GRR+CPG+TMA R +GL+LGLLIQCY WK G +KVDM EGGGITI K
Sbjct: 421 LLSNKLIPFGMGRRACPGETMAQRLIGLSLGLLIQCYDWKNVGYQKVDMTEGGGITIQKV 480
Query: 481 KPLEAMCKARPAMDKLLLNALD 501
+PLE +C+A P MDK+L N LD
Sbjct: 481 QPLETLCRALPIMDKVLSNGLD 499
BLAST of Sgr021310 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 563.9 bits (1452), Expect = 1.8e-159
Identity = 278/485 (57.32%), Postives = 359/485 (74.02%), Query Frame = 0
Query: 6 AFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGPIFSLR 65
+ L++T+ + + +NLPP P LPIIG+LH LK+P+H FH ++ +YG IFSL
Sbjct: 11 SLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLW 70
Query: 66 FGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLRRL 125
FGSRL V+VSSL IA+ECFTKND++ ANRP L K++GYN TT+A SPYGDHWRNLRR+
Sbjct: 71 FGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRI 130
Query: 126 AAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIVAG 185
+IEI S+ RLNS L IR+DEI RL+ KL S FT+VEL+ MFSE+ FN +MR+V+G
Sbjct: 131 LSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSG 190
Query: 186 KRYYGD--EVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKR 245
KRYYG+ +VSD EEAR FRG+++E+ GGA++ DF+ L+W G EK L +++KR
Sbjct: 191 KRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKR 250
Query: 246 TDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSA 305
TD F+Q LI+E R K N+++D LL Q S+PEYYTDQIIKGL++V+L AGTDTS+
Sbjct: 251 TDAFLQGLIDEHRFGK--RNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSS 310
Query: 306 VTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAP 365
VT+ WAM+ LLN+PE++ KAK ELDT IG DR VDE D+ L YLQ+IV ETLRLH AAP
Sbjct: 311 VTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAP 370
Query: 366 MLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKK 425
+L+ H SS D ++ GY+IP+ T L+VNAW IHRDP LW DPT F+PERF E + K
Sbjct: 371 LLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERF---EKEGEVNK 430
Query: 426 LIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLEA 485
L++FGLGRR+CPG+ ++ R GLTLGLLIQC++WK+ G+EK+DM E GIT K L A
Sbjct: 431 LLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKKTSLNA 490
Query: 486 MCKAR 489
MCK R
Sbjct: 491 MCKVR 490
BLAST of Sgr021310 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 563.9 bits (1452), Expect = 1.8e-159
Identity = 274/489 (56.03%), Postives = 368/489 (75.26%), Query Frame = 0
Query: 8 LLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEYGPIFSLRF 67
++LL L L + RRRNLPPSP +LP+IGHLHL+ + +HR+ + ++ +YG +FSL+
Sbjct: 13 VVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFSLQL 72
Query: 68 GSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLRRLA 127
G+RL ++VSS AEECFTKND++FANRP ++ K++GYN TTM SPYG+HWRNLRRLA
Sbjct: 73 GNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLRRLA 132
Query: 128 AIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIVAGK 187
A+EIFS LN LSIR+DE+++LLL L+ S +F KVE+K+ SEL+FN MR+VAGK
Sbjct: 133 AVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMVAGK 192
Query: 188 RYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKRTDK 247
RY+G +V D +EA+ FR L+ E+ H GAS+ DF+P L+WI +EK + ++++ D
Sbjct: 193 RYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSKISQEMDA 252
Query: 248 FMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSAVTL 307
F+Q LI E R K +++D LL LQ S+PEYYTDQIIKG+++VLL AGTDTSAVT+
Sbjct: 253 FLQRLIHESRINK---NNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTV 312
Query: 308 NWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAPMLL 367
WAM+ LLN+PE L KA+ E++T++G +R ++E DLP L+YL I+SET RL PAAPML+
Sbjct: 313 EWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPAAPMLV 372
Query: 368 SHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKKLIA 427
H SS DC V GYD+P+GT LLVNAWAIHRDP+ WD+PT F+PER G EL+ KL+
Sbjct: 373 PHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGV--ELEPSKLMP 432
Query: 428 FGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLEAMCK 487
FG+GRRSCPG +A R VGLTLG LIQC++WK+ G+ K+DM EG G+T+ KA+PLEA+CK
Sbjct: 433 FGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCK 492
Query: 488 ARPAMDKLL 496
R + K++
Sbjct: 493 PRNILHKVV 495
BLAST of Sgr021310 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 527.7 bits (1358), Expect = 1.4e-148
Identity = 261/488 (53.48%), Postives = 354/488 (72.54%), Query Frame = 0
Query: 8 LLLLLTVLILLQIRTRR-RNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGPIFSLRF 67
L ++T+ ILL+I +RR +NLPP PP++PIIG+LH LK P+HR F ++ YG IFSL F
Sbjct: 10 LSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWF 69
Query: 68 GSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLRRLA 127
GSRL V+VSS +A ECFTKND+I ANRPR L K++ YN TT+ ++ YGDHWRNLRR+
Sbjct: 70 GSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRIT 129
Query: 128 AIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGE---FTKVELKTMFSELAFNALMRIV 187
I++ S RLNS L +R+DE RL+ KL E FTKVEL+ +E+ FNA+MR++
Sbjct: 130 TIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMI 189
Query: 188 AGKRYYGD--EVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLT 247
+GKRYYGD +VSD EEA++FR ++ E+ GA++ DF+P+L+ + EK R+
Sbjct: 190 SGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIA 249
Query: 248 KRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDT 307
KR++ F++ LIEE R + +++D LL+L S+PEYY+D +IKGL+ +L AGTDT
Sbjct: 250 KRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDT 309
Query: 308 SAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPA 367
SAVT+ W M++LLN+PE+L KAK ELDT+IG+++ VDE DL L YLQ I+SETLRLHP
Sbjct: 310 SAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPP 369
Query: 368 APMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQS 427
AP+LL HYSS DCT+ +++P+ T +L N W IHRDPK W+D SF+PERF E +
Sbjct: 370 APLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF---EKEEEV 429
Query: 428 KKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPL 487
K++AFGLGRR+CPG ++A R VG T+GLLIQC++W++ +EK+DM EG GIT+ PL
Sbjct: 430 NKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMKIPL 489
Query: 488 EAMCKARP 490
AMCKA P
Sbjct: 490 RAMCKALP 494
BLAST of Sgr021310 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 526.6 bits (1355), Expect = 3.2e-148
Identity = 254/494 (51.42%), Postives = 361/494 (73.08%), Query Frame = 0
Query: 4 FSAFLLLLLTVLILLQIRTRR-RNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGPIF 63
+S F L L + ++ IR R+ +NLPP PPSLPIIG+LH LKRP+HR F ++ +YG +F
Sbjct: 9 YSVFYLALFFIFNIV-IRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVF 68
Query: 64 SLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNL 123
SL FGSRL V+VSS ++CFTKND++ ANRPR L K++ YN TT+ ++ YG+HWRNL
Sbjct: 69 SLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNL 128
Query: 124 RRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRI 183
RR+ A+++ S R+NS IR+DE QRL+ +L SS F ++EL + ++ FN +MR+
Sbjct: 129 RRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRM 188
Query: 184 VAGKRYYGD--EVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 243
++GKRYYG+ + SD +EA +FR ++ E+ GA++ DFMP+L+++ EK L +
Sbjct: 189 ISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDI 248
Query: 244 TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD 303
+ +TD F++ LIEE R KK ER N+++D LL LQ S+PEYYTDQIIKGL L +L AGTD
Sbjct: 249 SGKTDAFLRGLIEEHRTKK--ERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTD 308
Query: 304 TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP 363
+SAVTL W+M+ LLN+PE+L K K ELDT +GQDR VDE DLP L+YL+ +++ETLRL+
Sbjct: 309 SSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYT 368
Query: 364 AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ 423
AP+LL H +S +C + GY +P+ T +L+NAWAIHRDP+LW + T+F+PERF + +
Sbjct: 369 PAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERF---EKKGE 428
Query: 424 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP 483
+KLIAFG+GRR+CPG+ +A+R + +TL LLIQC+ WK +K+D+ E G T+ K P
Sbjct: 429 LEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVP 488
Query: 484 LEAMCKARPAMDKL 495
L+AMCK+RP ++K+
Sbjct: 489 LKAMCKSRPVINKV 496
BLAST of Sgr021310 vs. ExPASy Swiss-Prot
Match:
Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)
HSP 1 Score: 522.3 bits (1344), Expect = 6.0e-147
Identity = 263/493 (53.35%), Postives = 353/493 (71.60%), Query Frame = 0
Query: 7 FLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEY--GPIFS 66
F + L++ +L R R+ NLPPSP PIIGHLHLLK P+HR F ++ IFS
Sbjct: 19 FFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFS 78
Query: 67 LRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLR 126
L GSRL +VSS +AEECFTKND++ ANRP L+ KH+GYN TTM + YGD WRNLR
Sbjct: 79 LSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLR 138
Query: 127 RLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIV 186
R+ IEIFS+ RLNS +SIR+DEI+RL++ L S F KVE+K +F L N ++R+V
Sbjct: 139 RIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRMV 198
Query: 187 AGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKR 246
AGKR+YGD ++ EA+ R L+ E+ + GGA + D+ PIL+++ +EK + +L R
Sbjct: 199 AGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEKHVKKLAGR 258
Query: 247 TDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSA 306
D+F+Q+L+ E+R +KV + N+++D LL LQ ++P+YYTD IIKG++LV++ AGTDTSA
Sbjct: 259 VDEFLQSLVNEKRVEKV--KGNTMIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSA 318
Query: 307 VTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAP 366
TL WAM+ LLN+PE+L KAK E+D +IG DR V+E D+ L YLQ IVSETLRL+P AP
Sbjct: 319 GTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAP 378
Query: 367 MLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKK 426
MLL H +S DC V GYD+PRGT +LVNAWAIHRDPKLW++P F+PERF + + KK
Sbjct: 379 MLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERF---EKKGEDKK 438
Query: 427 LIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEG-GGITIHKAKPLE 486
L+ FG+GRRSCPG +A R V L LG L+QC++W++ ++ +DM E G T+ KA L+
Sbjct: 439 LMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQ 498
Query: 487 AMCKARPAMDKLL 496
AMCKARP + K+L
Sbjct: 499 AMCKARPIVHKVL 504
BLAST of Sgr021310 vs. ExPASy TrEMBL
Match:
K7NC01 (Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1)
HSP 1 Score: 997.3 bits (2577), Expect = 2.3e-287
Identity = 501/502 (99.80%), Postives = 501/502 (99.80%), Query Frame = 0
Query: 1 MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP
Sbjct: 1 MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
Query: 61 IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR
Sbjct: 61 IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM
Sbjct: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL
Sbjct: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
Query: 241 TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD 300
TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD
Sbjct: 241 TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD 300
Query: 301 TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP 360
TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP
Sbjct: 301 TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP 360
Query: 361 AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ 420
AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ
Sbjct: 361 AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ 420
Query: 421 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP 480
SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP
Sbjct: 421 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP 480
Query: 481 LEAMCKARPAMDKLLLNALDKI 503
LEAMCKARPAM KLLLNALDKI
Sbjct: 481 LEAMCKARPAMYKLLLNALDKI 502
BLAST of Sgr021310 vs. ExPASy TrEMBL
Match:
A0A6J1FUQ9 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=1)
HSP 1 Score: 770.4 bits (1988), Expect = 4.7e-219
Identity = 375/502 (74.70%), Postives = 437/502 (87.05%), Query Frame = 0
Query: 1 MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
MDF LLLLL L L Q TRRRNLPPSPPS PIIGHLHLL+RPIHRNFH IAA+YGP
Sbjct: 1 MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
Query: 61 IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
IF+LRFGSRLA+IVSSL +AEECFTKND++FANRPRLLI K+LGYN TT++T+P+GDHWR
Sbjct: 61 IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
NLRRL ++EIFSTARLN++LSIRKDEI+RLL+KLHS S F KVELK+MFSEL+FN +M
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVM 180
Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
R+V GKRY+G+EVS+E EAREFR LM+E S HGGAS+WVDFMPILKWIG G +EKSL +L
Sbjct: 181 RVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKL 240
Query: 241 TKRTDKFMQALIEERRNKKVL--ERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300
TKR DKFMQ L+E+RRN+ L E++++LLDRLLELQASEPEYY+D+IIKG++LVLLRAG
Sbjct: 241 TKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAG 300
Query: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360
D+++VT+ WAM QLLNNPE+++KAKAE+DTKIG+DR +DE DL NL YLQAI+SET RL
Sbjct: 301 ADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRL 360
Query: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420
HPAAPMLL+HYSS DCTVAGYD+PRGT LLVNAWAIHRDPKLWDDP SFRPERFLG NE
Sbjct: 361 HPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNE 420
Query: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480
L KLI FG+GRR+CPG+TMALR +GL+LGLLIQCY+WK G EKVDM EGGGITI K
Sbjct: 421 LLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKV 480
Query: 481 KPLEAMCKARPAMDKLLLNALD 501
+PLE MC+A P MDK+L N LD
Sbjct: 481 QPLETMCRALPIMDKVLSNGLD 499
BLAST of Sgr021310 vs. ExPASy TrEMBL
Match:
A0A6J1J5B5 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1)
HSP 1 Score: 767.3 bits (1980), Expect = 3.9e-218
Identity = 373/502 (74.30%), Postives = 439/502 (87.45%), Query Frame = 0
Query: 1 MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
MDF S FLLLLL L L Q TRRRNLPPSPPS+PIIGHLHLL+RPIH+NFH IAA+YGP
Sbjct: 1 MDFLSVFLLLLLVFLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAKYGP 60
Query: 61 IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
IF+LRFGSRLA+IVSSL +AEECFTKND++FANRPRLLI K+LGYN TT++T+PYGD WR
Sbjct: 61 IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDLWR 120
Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
NLRRL ++E+FSTARLN++LSIRKDEI+RLL+KLHS S F KVELK+MFSEL+FN +M
Sbjct: 121 NLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVM 180
Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
R+V GKRY+G+EVS+E EAREFR LM+EIS HGGAS+W++FMPILKWIG G +EKSL +L
Sbjct: 181 RVVTGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPILKWIGFGEYEKSLAKL 240
Query: 241 TKRTDKFMQALIEERRNKK--VLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300
TKR DKFMQ L+E RRN+ +E++++LLDRLLELQASEPEYY+D+IIKG+VLVLLRAG
Sbjct: 241 TKRADKFMQKLVEYRRNQTGFEIEKQSTLLDRLLELQASEPEYYSDEIIKGVVLVLLRAG 300
Query: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360
D+++VT+ WAM QLLNNPE+++KAKAE+DTKIG+DR VDE DL NL YLQAI+ ET RL
Sbjct: 301 VDSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETFRL 360
Query: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420
HPAAPMLL+HYSS DCTVAGYDIPRGT L+VNAWAIHRDPKLWDDP SFRPERFLGA NE
Sbjct: 361 HPAAPMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAGNE 420
Query: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480
L S KL+ FG+GRR+CPG+TMA R +GL+LGLLIQCY+WK G +KVDM EGGGITI K
Sbjct: 421 LLSNKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQKV 480
Query: 481 KPLEAMCKARPAMDKLLLNALD 501
+PLE MC+A P MDK+L N LD
Sbjct: 481 QPLETMCRALPIMDKVLSNGLD 499
BLAST of Sgr021310 vs. ExPASy TrEMBL
Match:
A0A6J1D806 (cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)
HSP 1 Score: 765.8 bits (1976), Expect = 1.1e-217
Identity = 389/512 (75.98%), Postives = 436/512 (85.16%), Query Frame = 0
Query: 1 MDFFSAFLLLLL---TVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAE 60
MD S F LLL ++L QIR RRNLPPSPPS+PIIGHL LL+RP+HRNF KIAAE
Sbjct: 1 MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
Query: 61 YGPIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGD 120
YGPIFSLRFGSRLAV+VSSLD AEEC TKND++FANRPRL+I KHLGYN TTMA +PYG
Sbjct: 61 YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
Query: 121 HWRNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLH----SGSSGEFTKVELKTMFSE 180
HWRNLRRL A E+FSTARL SSLSIRK+EI+R+LLKL SGS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
Query: 181 LAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIG-GGG 240
+ FN +MR+VAGKRYYG+EVSDE EAREF LMEEIS HGGAS+WVDFMPIL+WIG GG
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
Query: 241 FEKSLVRLTKRTDKFMQALIEE-RRNKK--VLERKN--SLLDRLLELQASEPEYYTDQII 300
+E++L +L KRTDKFM+AL+EE R NKK +ERKN SLLDRLLELQ SEP+YYTDQI+
Sbjct: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
Query: 301 KGLVLVLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSY 360
KGLVLVLLRAGTD+++VT+NWAM QLLNNPE+L KAKAELDTK+GQDR VDE DL NL Y
Sbjct: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360
Query: 361 LQAIVSETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSF 420
LQAIV ETLRLHP APML++HYSS+DCTVAGY IP T LLVNAWAIHRDPKLWDDPTSF
Sbjct: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420
Query: 421 RPERFLG-AANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVD 480
RPERFLG AANEL S KLIAFGLGRR+CPG TM RFVGLTL L+IQCY+WKK G+E +D
Sbjct: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480
Query: 481 MGEGGGITIHKAKPLEAMCKARPAMDKLLLNA 499
MGEGGGITI KAKPLE MCKARP MDK+ + A
Sbjct: 481 MGEGGGITILKAKPLEVMCKARPVMDKVHVKA 512
BLAST of Sgr021310 vs. ExPASy TrEMBL
Match:
A0A6J1D8F1 (cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)
HSP 1 Score: 760.4 bits (1962), Expect = 4.8e-216
Identity = 389/515 (75.53%), Postives = 436/515 (84.66%), Query Frame = 0
Query: 1 MDFFSAFLLLLL---TVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAE 60
MD S F LLL ++L QIR RRNLPPSPPS+PIIGHL LL+RP+HRNF KIAAE
Sbjct: 1 MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
Query: 61 YGPIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGD 120
YGPIFSLRFGSRLAV+VSSLD AEEC TKND++FANRPRL+I KHLGYN TTMA +PYG
Sbjct: 61 YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
Query: 121 HWRNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLH----SGSSGEFTKVELKTMFSE 180
HWRNLRRL A E+FSTARL SSLSIRK+EI+R+LLKL SGS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
Query: 181 LAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIG-GGG 240
+ FN +MR+VAGKRYYG+EVSDE EAREF LMEEIS HGGAS+WVDFMPIL+WIG GG
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
Query: 241 FEKSLVRLTKRTDKFMQALIEE-RRNKK--VLERKN--SLLDRLLELQASEPEYYTDQII 300
+E++L +L KRTDKFM+AL+EE R NKK +ERKN SLLDRLLELQ SEP+YYTDQI+
Sbjct: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
Query: 301 KGLVL---VLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPN 360
KGLVL VLLRAGTD+++VT+NWAM QLLNNPE+L KAKAELDTK+GQDR VDE DL N
Sbjct: 301 KGLVLNMQVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN 360
Query: 361 LSYLQAIVSETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDP 420
L YLQAIV ETLRLHP APML++HYSS+DCTVAGY IP T LLVNAWAIHRDPKLWDDP
Sbjct: 361 LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP 420
Query: 421 TSFRPERFLG-AANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDE 480
TSFRPERFLG AANEL S KLIAFGLGRR+CPG TM RFVGLTL L+IQCY+WKK G+E
Sbjct: 421 TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE 480
Query: 481 KVDMGEGGGITIHKAKPLEAMCKARPAMDKLLLNA 499
+DMGEGGGITI KAKPLE MCKARP MDK+ + A
Sbjct: 481 NIDMGEGGGITILKAKPLEVMCKARPVMDKVHVKA 515
BLAST of Sgr021310 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 549.7 bits (1415), Expect = 2.5e-156
Identity = 274/492 (55.69%), Postives = 368/492 (74.80%), Query Frame = 0
Query: 6 AFLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEY--GPIF 65
+ L ++L+++ L+ R+ NLPPSP SLP+IGHL LLK PIHR F ++ PIF
Sbjct: 9 SILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIF 68
Query: 66 SLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNL 125
SLR G+RL + SS IAEECFTKND++ ANRP +++KH+ Y+ TTM + YGDHWRNL
Sbjct: 69 SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 128
Query: 126 RRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRI 185
RR+ ++EIFS RLNS LSIRKDEI+RL+ +L S EF KV++K+M S+L FN ++R+
Sbjct: 129 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRM 188
Query: 186 VAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTK 245
VAGKRYYGD V D+ EA+ R L+ ++ GA + VD++P+L+ + +E + +L
Sbjct: 189 VAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLAG 248
Query: 246 RTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTS 305
R D+F+Q L++E+R K E+ N+++D LL LQ S+P+Y+TD+IIKG +L L+ AGTDTS
Sbjct: 249 RLDEFLQGLVDEKREAK--EKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTS 308
Query: 306 AVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAA 365
AVTL WA++ +LN+P++L KA+ E+D KIG DR +DE D+ NL YLQ IVSETLRL+PAA
Sbjct: 309 AVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAA 368
Query: 366 PMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSK 425
PMLL H +S DC VAGYD+PRGT LL N WAIHRDP+LWDDP SF+PERF E +++
Sbjct: 369 PMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERF---EKEGEAQ 428
Query: 426 KLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLE 485
KL+ FGLGRR+CPG +A R + LTLG LIQC +W+K G+E VDM EG G+T+ KAKPLE
Sbjct: 429 KLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLE 488
Query: 486 AMCKARPAMDKL 495
AMC+ARP++ K+
Sbjct: 489 AMCRARPSVVKI 492
BLAST of Sgr021310 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 540.8 bits (1392), Expect = 1.2e-153
Identity = 273/493 (55.38%), Postives = 362/493 (73.43%), Query Frame = 0
Query: 7 FLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEYG--PIFS 66
FL + L++ ++ RR NLPPSP +LP+IGHL LLK P+HR F ++ G PI S
Sbjct: 10 FLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIIS 69
Query: 67 LRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLR 126
LR G+RL +VSS +AEECFTKND++ ANR L SKH+ Y CTT+ T+ YGDHWRNLR
Sbjct: 70 LRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLR 129
Query: 127 RLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIV 186
R+ A+EIFS RLNS SIR+DEI RL+ L SS EFTKVE+K+MFS L FN ++R++
Sbjct: 130 RIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRML 189
Query: 187 AGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKR 246
AGK YYGD D+ EA+ R L+ E GA + D++PIL WI G EK + ++ R
Sbjct: 190 AGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWITGS--EKRIKKIASR 249
Query: 247 TDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSA 306
D+F+Q L++ERR K +R+N+++D LL LQ ++PEYYTD IIKG++L L+ AGTDTSA
Sbjct: 250 LDEFLQGLVDERREGKE-KRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSA 309
Query: 307 VTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAP 366
VTL W ++ LLN+P++L+KA+ E+D K+G +R V+E DL +L YLQ IVSE+LRL+PA+P
Sbjct: 310 VTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASP 369
Query: 367 MLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKK 426
+L+ H +S DC V GY +PRGT LL NAWAIHRDPK+WDDPTSF+PERF E +++K
Sbjct: 370 LLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERF---EKEGEAQK 429
Query: 427 LIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGE-GGGITIHKAKPLE 486
L+ FGLGRR+CPG +A R LT+G LIQC++W++ G+E+VDM E GGG+ + KA PL
Sbjct: 430 LLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLV 489
Query: 487 AMCKARPAMDKLL 496
AMCKARP + K+L
Sbjct: 490 AMCKARPVVGKIL 496
BLAST of Sgr021310 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 530.0 bits (1364), Expect = 2.0e-150
Identity = 269/492 (54.67%), Postives = 360/492 (73.17%), Query Frame = 0
Query: 7 FLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEYG--PIFS 66
F ++L+++ L+ R+ NLPPSP +LP+IGHL LLK P+HR F ++ G PI S
Sbjct: 10 FCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIIS 69
Query: 67 LRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLR 126
LR G+RL +VSS IAEECFTKND+I ANR + +KH+ Y +T+ ++ Y +HWRNLR
Sbjct: 70 LRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLR 129
Query: 127 RLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIV 186
R+ A+EIFS RLNS SIR+DEI+RL+ +L SS FTKVE+K+MFS+L FN ++R++
Sbjct: 130 RIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIRML 189
Query: 187 AGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKR 246
AGK YYGD D+ EA+ R L+ E G + D++PIL WI E + +L R
Sbjct: 190 AGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRIKKLAGR 249
Query: 247 TDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSA 306
D+F+Q L++E+R K +++N+++D LL LQ ++PEYY D+IIKG +L L+ GTDT+A
Sbjct: 250 LDEFLQGLVDEKREGKE-KKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTA 309
Query: 307 VTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAP 366
VTL WA++ LLNNPE+L KA+ E+D IG DR ++E D+PNL YLQ IVSETLRL+PAAP
Sbjct: 310 VTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAP 369
Query: 367 MLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKK 426
MLL H +S DC V GYD+PRGT LL NAWAIHRDP LWDDPTSF+PERF E ++KK
Sbjct: 370 MLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERF---EKEGEAKK 429
Query: 427 LIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLEA 486
L+ FGLGRR+CPG +A R V L+LG LIQC++W++ G+E+VDM EG G+T+ KA+PLEA
Sbjct: 430 LMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEA 489
Query: 487 MCKARPAMDKLL 496
MC+AR + K+L
Sbjct: 490 MCRARDFVGKIL 495
BLAST of Sgr021310 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 530.0 bits (1364), Expect = 2.0e-150
Identity = 264/493 (53.55%), Postives = 353/493 (71.60%), Query Frame = 0
Query: 6 AFLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEYG--PIF 65
+FL L +++ +LL R+ NLPPSP LP+IGHLHLLK+P+HR FH I+ G PIF
Sbjct: 10 SFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIF 69
Query: 66 SLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNL 125
LR G+RL ++SS IAEECFTKND++ ANRP ++++KH+GYN T M + YGDHWRNL
Sbjct: 70 HLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNL 129
Query: 126 RRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRI 185
RR+AA+EIFS+ R+++ SIRKDEI+RL+ L S F +VELK++ + LAFN ++ +
Sbjct: 130 RRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIMM 189
Query: 186 VAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTK 245
VAGKRYYG D +EA+ R L+ EI G+ + D++P + W+ FE L
Sbjct: 190 VAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKILGN 249
Query: 246 RTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTS 305
R D+ +Q L++E+R +K E+ +L+D LL Q +EPEYYTD IIKG++L L+ AGTDTS
Sbjct: 250 RLDRVLQKLVDEKRAEK--EKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAGTDTS 309
Query: 306 AVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAA 365
+VTL WAM+ LLN+PE+L KA+AE+D KIG DR V+E D+ NL YLQ IVSETLRL+PA
Sbjct: 310 SVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPAV 369
Query: 366 PMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSK 425
P+LL H+SS +C VAGYD+PR T LL N WA+HRDP LW++P F+PERF E +++
Sbjct: 370 PLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERF---EKEGEAR 429
Query: 426 KLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLE 485
KL+ FG+GRR+CPG + R V L LG LIQ ++W++ G E VDM EG GIT+ KA PL
Sbjct: 430 KLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPKATPLR 489
Query: 486 AMCKARPAMDKLL 496
AMCKAR + K +
Sbjct: 490 AMCKARAIVGKTI 495
BLAST of Sgr021310 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 526.6 bits (1355), Expect = 2.3e-149
Identity = 262/493 (53.14%), Postives = 355/493 (72.01%), Query Frame = 0
Query: 6 AFLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEYG--PIF 65
+FL L + LL R+ NLPPSP SLP+IGHLHLLK P+HR F ++ G P+F
Sbjct: 10 SFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVF 69
Query: 66 SLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNL 125
LR G+RL ++SS IAEECFTKND++ ANRP+ ISKHLGYN T + ++ YGDHWRNL
Sbjct: 70 HLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNL 129
Query: 126 RRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRI 185
RR+AA+EIFST RLNS L IRKDEI+RL+ L S F +VE+KT+ + LA N +R+
Sbjct: 130 RRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTTIRM 189
Query: 186 VAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTK 245
+AGKRY+G+ D ++A+ + L+ E GA + +D++ IL+W+ +EK + L
Sbjct: 190 LAGKRYFGE---DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIKNLGN 249
Query: 246 RTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTS 305
R D F+Q L++E+R +K E+ +++D LL LQ +P+YYTD IIKG++L L+ AGTDTS
Sbjct: 250 RFDTFLQKLVDEKRAEK--EKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAGTDTS 309
Query: 306 AVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAA 365
+VTL WAM+ LLN+PE+L KA+ E+D K+G DR VDE D+ NLSYLQ+IV ETLR++PA
Sbjct: 310 SVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMYPAV 369
Query: 366 PMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSK 425
P+LL H SS DC V GYDIP GT +L NAWA+HRDP++W+DP F+PERF E +++
Sbjct: 370 PLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERF---EKEGEAE 429
Query: 426 KLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLE 485
KLI+FG+GRR+CPG +A R + LG L+QC++W++ G++ VDM E G T+ KA PL
Sbjct: 430 KLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAIPLR 489
Query: 486 AMCKARPAMDKLL 496
AMCKAR +DKL+
Sbjct: 490 AMCKARSIVDKLI 492
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
AEM42992.1 | 4.8e-287 | 99.80 | cytochrome P450 [Siraitia grosvenorii] | [more] |
XP_022944057.1 | 9.6e-219 | 74.70 | cytochrome P450 81E8-like [Cucurbita moschata] >KAG7011030.1 Cytochrome P450 81E... | [more] |
XP_022985607.1 | 8.1e-218 | 74.30 | cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E... | [more] |
XP_022149457.1 | 2.4e-217 | 75.98 | cytochrome P450 81E8-like isoform X2 [Momordica charantia] | [more] |
XP_023512643.1 | 2.4e-217 | 74.30 | cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
Q6WNQ8 | 1.8e-159 | 57.32 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
W8JMU7 | 1.8e-159 | 56.03 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q6WNQ9 | 1.4e-148 | 53.48 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
P93147 | 3.2e-148 | 51.42 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q9LHA1 | 6.0e-147 | 53.35 | Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
K7NC01 | 2.3e-287 | 99.80 | Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1 | [more] |
A0A6J1FUQ9 | 4.7e-219 | 74.70 | cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=... | [more] |
A0A6J1J5B5 | 3.9e-218 | 74.30 | cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1 | [more] |
A0A6J1D806 | 1.1e-217 | 75.98 | cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017... | [more] |
A0A6J1D8F1 | 4.8e-216 | 75.53 | cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017... | [more] |
Match Name | E-value | Identity | Description | |
AT4G37370.1 | 2.5e-156 | 55.69 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37340.1 | 1.2e-153 | 55.38 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
AT4G37360.1 | 2.0e-150 | 54.67 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT4G37320.1 | 2.0e-150 | 53.55 | cytochrome P450, family 81, subfamily D, polypeptide 5 | [more] |
AT4G37330.1 | 2.3e-149 | 53.14 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |