Sgr021310 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr021310
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionCytochrome P450
Locationtig00153654: 842534 .. 844873 (-)
RNA-Seq ExpressionSgr021310
SyntenySgr021310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTTTTTCTCTGCTTTCCTTCTACTCCTCCTCACTGTCCTTATTCTGCTGCAGATCAGAACCCGTCGGAGAAATCTGCCGCCGAGCCCGCCGTCTCTTCCGATCATCGGCCACCTCCACCTCCTCAAACGACCCATCCACCGTAACTTCCACAAGATTGCAGCCGAATACGGTCCCATTTTCTCTCTCAGGTTCGGATCTCGCCTCGCGGTTATAGTTTCTTCGCTCGATATAGCCGAAGAATGTTTCACCAAGAACGACCTCATTTTCGCCAACCGGCCACGTTTGCTCATCAGTAAACATCTCGGCTACAACTGCACCACCATGGCGACCTCGCCCTACGGTGACCACTGGCGGAACCTCCGCCGCCTTGCGGCCATCGAGATCTTCTCCACGGCTCGGCTGAACTCATCTCTGAGCATCCGGAAGGACGAAATCCAGCGCTTGTTACTCAAACTCCATTCCGGATCGAGCGGGGAGTTCACCAAGGTCGAGTTGAAAACGATGTTTTCGGAGCTGGCTTTCAACGCCTTGATGAGGATCGTCGCCGGGAAACGGTACTACGGCGATGAGGTTTCTGATGAGGAGGAGGCGAGGGAGTTCAGAGGGCTTATGGAGGAGATCTCACTTCATGGAGGAGCATCGCATTGGGTTGATTTCATGCCGATTCTGAAATGGATTGGCGGAGGCGGATTTGAGAAGAGTTTGGTGAGGCTGACGAAGAGGACGGATAAATTTATGCAGGCACTCATTGAAGAACGACGCAACAAGAAAGTGTTAGAAAGAAAGAATTCTCTGCTTGATCGTCTTCTGGAATTGCAGGCGTCGGAACCAGAATATTACACCGATCAGATCATCAAAGGCCTCGTTCTGGTAAGCAATCGAATGGAATTTTTTGTTTCTTCGTTGAATTTTCTTAGCGGAAGACTGATACAGACTTAATCCGAGTTGAATTGAATATCCGAAACTCTATGAACTACGAAGAACAGTAAGCAAAAGGGTTAGAATCTAGTTGACTTAATTCACTTTCTTTCTTAACTTGAAGTCAAATTTTGTTATGTTACCAGTAATCATGAAAGGGAAAGAATTTTAAATCAAGTGATTTGAAAATTTGGGAATGATTTTAAACTTGTTATTTTTATGTGGAATGAGAAAAGTTTGAAATAAATGTAATTAGAAATTTTTGTTTAAATGTTGGAAAAATGAAAAAGAAAATAAGAATTTTATTAATATAGCCTTCCAATTCATAATTTGCTAATACAAAAAATTAAAATTTGATGATGTTACATCAATGATTGAATTTGGAATTAAAATGCAAACATCAAAATTTATTTTAAATCTATTATGATTTGAAATCAATTAAAATCTTTCAATCCAAACATAGCTCAAGGATAAGACACGTATTACCATTCCAAAGATCAATGTTCGATCTTTCATTCCGCAATTGTTGAACTAAAAAAAAAAATCAATTAAAATCTTGAAGAATTTTCTGTCTTATGATAAAAATGTGACCACCTCATGATTATTAGATGCATAGGTAAAATAATTTTTTAATTTTTCTATTTATATACCTCTGGCCATATTGTTTCAGCCTTAAAGAAGAATTTTATTCCCATTCTGAAGTTTGGTCGTTAGAATCTGATAAATTAAAAAAAGAAAAAGAAAAAGAGGATTTTATTATTTAATGAAGTAGAAAATGGGCAGGTTTTGTTGCGAGCGGGAACGGACACGTCAGCTGTGACATTGAATTGGGCAATGGCCCAACTGCTCAACAATCCGGAGCTGCTGGCCAAAGCCAAAGCCGAGTTGGACACCAAAATCGGTCAAGACCGACCGGTTGACGAACCGGATCTCCCCAACCTCAGCTACCTTCAAGCCATTGTTTCCGAGACCCTCCGTCTGCACCCCGCTGCTCCGATGCTCCTCTCACATTACTCCTCCGCCGATTGCACCGTCGCCGGCTACGATATCCCACGTGGCACGACGTTGTTGGTCAATGCATGGGCGATTCACAGAGACCCGAAGCTGTGGGACGACCCGACGAGCTTCCGGCCGGAGAGGTTCTTGGGAGCGGCGAATGAGTTGCAGTCCAAGAAACTAATTGCTTTTGGGCTTGGGAGGAGGTCTTGTCCCGGAGATACTATGGCTCTACGTTTTGTGGGACTCACTCTGGGGCTGTTGATTCAGTGTTATCAGTGGAAAAAGTGTGGAGATGAAAAGGTCGACATGGGGGAAGGTGGGGGTATTACCATTCACAAAGCGAAGCCTTTGGAAGCCATGTGTAAAGCTCGCCCTGCCATGGATAAACTTCTGTTAAATGCTCTGGACAAAATTTGA

mRNA sequence

ATGGACTTTTTCTCTGCTTTCCTTCTACTCCTCCTCACTGTCCTTATTCTGCTGCAGATCAGAACCCGTCGGAGAAATCTGCCGCCGAGCCCGCCGTCTCTTCCGATCATCGGCCACCTCCACCTCCTCAAACGACCCATCCACCGTAACTTCCACAAGATTGCAGCCGAATACGGTCCCATTTTCTCTCTCAGGTTCGGATCTCGCCTCGCGGTTATAGTTTCTTCGCTCGATATAGCCGAAGAATGTTTCACCAAGAACGACCTCATTTTCGCCAACCGGCCACGTTTGCTCATCAGTAAACATCTCGGCTACAACTGCACCACCATGGCGACCTCGCCCTACGGTGACCACTGGCGGAACCTCCGCCGCCTTGCGGCCATCGAGATCTTCTCCACGGCTCGGCTGAACTCATCTCTGAGCATCCGGAAGGACGAAATCCAGCGCTTGTTACTCAAACTCCATTCCGGATCGAGCGGGGAGTTCACCAAGGTCGAGTTGAAAACGATGTTTTCGGAGCTGGCTTTCAACGCCTTGATGAGGATCGTCGCCGGGAAACGGTACTACGGCGATGAGGTTTCTGATGAGGAGGAGGCGAGGGAGTTCAGAGGGCTTATGGAGGAGATCTCACTTCATGGAGGAGCATCGCATTGGGTTGATTTCATGCCGATTCTGAAATGGATTGGCGGAGGCGGATTTGAGAAGAGTTTGGTGAGGCTGACGAAGAGGACGGATAAATTTATGCAGGCACTCATTGAAGAACGACGCAACAAGAAAGTGTTAGAAAGAAAGAATTCTCTGCTTGATCGTCTTCTGGAATTGCAGGCGTCGGAACCAGAATATTACACCGATCAGATCATCAAAGGCCTCGTTCTGGTTTTGTTGCGAGCGGGAACGGACACGTCAGCTGTGACATTGAATTGGGCAATGGCCCAACTGCTCAACAATCCGGAGCTGCTGGCCAAAGCCAAAGCCGAGTTGGACACCAAAATCGGTCAAGACCGACCGGTTGACGAACCGGATCTCCCCAACCTCAGCTACCTTCAAGCCATTGTTTCCGAGACCCTCCGTCTGCACCCCGCTGCTCCGATGCTCCTCTCACATTACTCCTCCGCCGATTGCACCGTCGCCGGCTACGATATCCCACGTGGCACGACGTTGTTGGTCAATGCATGGGCGATTCACAGAGACCCGAAGCTGTGGGACGACCCGACGAGCTTCCGGCCGGAGAGGTTCTTGGGAGCGGCGAATGAGTTGCAGTCCAAGAAACTAATTGCTTTTGGGCTTGGGAGGAGGTCTTGTCCCGGAGATACTATGGCTCTACGTTTTGTGGGACTCACTCTGGGGCTGTTGATTCAGTGTTATCAGTGGAAAAAGTGTGGAGATGAAAAGGTCGACATGGGGGAAGGTGGGGGTATTACCATTCACAAAGCGAAGCCTTTGGAAGCCATGTGTAAAGCTCGCCCTGCCATGGATAAACTTCTGTTAAATGCTCTGGACAAAATTTGA

Coding sequence (CDS)

ATGGACTTTTTCTCTGCTTTCCTTCTACTCCTCCTCACTGTCCTTATTCTGCTGCAGATCAGAACCCGTCGGAGAAATCTGCCGCCGAGCCCGCCGTCTCTTCCGATCATCGGCCACCTCCACCTCCTCAAACGACCCATCCACCGTAACTTCCACAAGATTGCAGCCGAATACGGTCCCATTTTCTCTCTCAGGTTCGGATCTCGCCTCGCGGTTATAGTTTCTTCGCTCGATATAGCCGAAGAATGTTTCACCAAGAACGACCTCATTTTCGCCAACCGGCCACGTTTGCTCATCAGTAAACATCTCGGCTACAACTGCACCACCATGGCGACCTCGCCCTACGGTGACCACTGGCGGAACCTCCGCCGCCTTGCGGCCATCGAGATCTTCTCCACGGCTCGGCTGAACTCATCTCTGAGCATCCGGAAGGACGAAATCCAGCGCTTGTTACTCAAACTCCATTCCGGATCGAGCGGGGAGTTCACCAAGGTCGAGTTGAAAACGATGTTTTCGGAGCTGGCTTTCAACGCCTTGATGAGGATCGTCGCCGGGAAACGGTACTACGGCGATGAGGTTTCTGATGAGGAGGAGGCGAGGGAGTTCAGAGGGCTTATGGAGGAGATCTCACTTCATGGAGGAGCATCGCATTGGGTTGATTTCATGCCGATTCTGAAATGGATTGGCGGAGGCGGATTTGAGAAGAGTTTGGTGAGGCTGACGAAGAGGACGGATAAATTTATGCAGGCACTCATTGAAGAACGACGCAACAAGAAAGTGTTAGAAAGAAAGAATTCTCTGCTTGATCGTCTTCTGGAATTGCAGGCGTCGGAACCAGAATATTACACCGATCAGATCATCAAAGGCCTCGTTCTGGTTTTGTTGCGAGCGGGAACGGACACGTCAGCTGTGACATTGAATTGGGCAATGGCCCAACTGCTCAACAATCCGGAGCTGCTGGCCAAAGCCAAAGCCGAGTTGGACACCAAAATCGGTCAAGACCGACCGGTTGACGAACCGGATCTCCCCAACCTCAGCTACCTTCAAGCCATTGTTTCCGAGACCCTCCGTCTGCACCCCGCTGCTCCGATGCTCCTCTCACATTACTCCTCCGCCGATTGCACCGTCGCCGGCTACGATATCCCACGTGGCACGACGTTGTTGGTCAATGCATGGGCGATTCACAGAGACCCGAAGCTGTGGGACGACCCGACGAGCTTCCGGCCGGAGAGGTTCTTGGGAGCGGCGAATGAGTTGCAGTCCAAGAAACTAATTGCTTTTGGGCTTGGGAGGAGGTCTTGTCCCGGAGATACTATGGCTCTACGTTTTGTGGGACTCACTCTGGGGCTGTTGATTCAGTGTTATCAGTGGAAAAAGTGTGGAGATGAAAAGGTCGACATGGGGGAAGGTGGGGGTATTACCATTCACAAAGCGAAGCCTTTGGAAGCCATGTGTAAAGCTCGCCCTGCCATGGATAAACTTCTGTTAAATGCTCTGGACAAAATTTGA

Protein sequence

MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGPIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLEAMCKARPAMDKLLLNALDKI
Homology
BLAST of Sgr021310 vs. NCBI nr
Match: AEM42992.1 (cytochrome P450 [Siraitia grosvenorii])

HSP 1 Score: 997.3 bits (2577), Expect = 4.8e-287
Identity = 501/502 (99.80%), Postives = 501/502 (99.80%), Query Frame = 0

Query: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
           MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP
Sbjct: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60

Query: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
           IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR
Sbjct: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120

Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
           NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM
Sbjct: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180

Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
           RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL
Sbjct: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240

Query: 241 TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD 300
           TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD
Sbjct: 241 TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD 300

Query: 301 TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP 360
           TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP
Sbjct: 301 TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP 360

Query: 361 AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ 420
           AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ
Sbjct: 361 AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ 420

Query: 421 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP 480
           SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP
Sbjct: 421 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP 480

Query: 481 LEAMCKARPAMDKLLLNALDKI 503
           LEAMCKARPAM KLLLNALDKI
Sbjct: 481 LEAMCKARPAMYKLLLNALDKI 502

BLAST of Sgr021310 vs. NCBI nr
Match: XP_022944057.1 (cytochrome P450 81E8-like [Cucurbita moschata] >KAG7011030.1 Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 770.4 bits (1988), Expect = 9.6e-219
Identity = 375/502 (74.70%), Postives = 437/502 (87.05%), Query Frame = 0

Query: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
           MDF    LLLLL  L L Q  TRRRNLPPSPPS PIIGHLHLL+RPIHRNFH IAA+YGP
Sbjct: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60

Query: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
           IF+LRFGSRLA+IVSSL +AEECFTKND++FANRPRLLI K+LGYN TT++T+P+GDHWR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120

Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
           NLRRL ++EIFSTARLN++LSIRKDEI+RLL+KLHS S   F KVELK+MFSEL+FN +M
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVM 180

Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
           R+V GKRY+G+EVS+E EAREFR LM+E S HGGAS+WVDFMPILKWIG G +EKSL +L
Sbjct: 181 RVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKL 240

Query: 241 TKRTDKFMQALIEERRNKKVL--ERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300
           TKR DKFMQ L+E+RRN+  L  E++++LLDRLLELQASEPEYY+D+IIKG++LVLLRAG
Sbjct: 241 TKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAG 300

Query: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360
            D+++VT+ WAM QLLNNPE+++KAKAE+DTKIG+DR +DE DL NL YLQAI+SET RL
Sbjct: 301 ADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRL 360

Query: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420
           HPAAPMLL+HYSS DCTVAGYD+PRGT LLVNAWAIHRDPKLWDDP SFRPERFLG  NE
Sbjct: 361 HPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNE 420

Query: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480
           L   KLI FG+GRR+CPG+TMALR +GL+LGLLIQCY+WK  G EKVDM EGGGITI K 
Sbjct: 421 LLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKV 480

Query: 481 KPLEAMCKARPAMDKLLLNALD 501
           +PLE MC+A P MDK+L N LD
Sbjct: 481 QPLETMCRALPIMDKVLSNGLD 499

BLAST of Sgr021310 vs. NCBI nr
Match: XP_022985607.1 (cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E8-like [Cucurbita maxima])

HSP 1 Score: 767.3 bits (1980), Expect = 8.1e-218
Identity = 373/502 (74.30%), Postives = 439/502 (87.45%), Query Frame = 0

Query: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
           MDF S FLLLLL  L L Q  TRRRNLPPSPPS+PIIGHLHLL+RPIH+NFH IAA+YGP
Sbjct: 1   MDFLSVFLLLLLVFLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAKYGP 60

Query: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
           IF+LRFGSRLA+IVSSL +AEECFTKND++FANRPRLLI K+LGYN TT++T+PYGD WR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDLWR 120

Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
           NLRRL ++E+FSTARLN++LSIRKDEI+RLL+KLHS S   F KVELK+MFSEL+FN +M
Sbjct: 121 NLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVM 180

Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
           R+V GKRY+G+EVS+E EAREFR LM+EIS HGGAS+W++FMPILKWIG G +EKSL +L
Sbjct: 181 RVVTGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPILKWIGFGEYEKSLAKL 240

Query: 241 TKRTDKFMQALIEERRNKK--VLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300
           TKR DKFMQ L+E RRN+    +E++++LLDRLLELQASEPEYY+D+IIKG+VLVLLRAG
Sbjct: 241 TKRADKFMQKLVEYRRNQTGFEIEKQSTLLDRLLELQASEPEYYSDEIIKGVVLVLLRAG 300

Query: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360
            D+++VT+ WAM QLLNNPE+++KAKAE+DTKIG+DR VDE DL NL YLQAI+ ET RL
Sbjct: 301 VDSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETFRL 360

Query: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420
           HPAAPMLL+HYSS DCTVAGYDIPRGT L+VNAWAIHRDPKLWDDP SFRPERFLGA NE
Sbjct: 361 HPAAPMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAGNE 420

Query: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480
           L S KL+ FG+GRR+CPG+TMA R +GL+LGLLIQCY+WK  G +KVDM EGGGITI K 
Sbjct: 421 LLSNKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQKV 480

Query: 481 KPLEAMCKARPAMDKLLLNALD 501
           +PLE MC+A P MDK+L N LD
Sbjct: 481 QPLETMCRALPIMDKVLSNGLD 499

BLAST of Sgr021310 vs. NCBI nr
Match: XP_022149457.1 (cytochrome P450 81E8-like isoform X2 [Momordica charantia])

HSP 1 Score: 765.8 bits (1976), Expect = 2.4e-217
Identity = 389/512 (75.98%), Postives = 436/512 (85.16%), Query Frame = 0

Query: 1   MDFFSAFLLLLL---TVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAE 60
           MD  S F LLL    ++L   QIR  RRNLPPSPPS+PIIGHL LL+RP+HRNF KIAAE
Sbjct: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60

Query: 61  YGPIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGD 120
           YGPIFSLRFGSRLAV+VSSLD AEEC TKND++FANRPRL+I KHLGYN TTMA +PYG 
Sbjct: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120

Query: 121 HWRNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLH----SGSSGEFTKVELKTMFSE 180
           HWRNLRRL A E+FSTARL SSLSIRK+EI+R+LLKL     SGS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180

Query: 181 LAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIG-GGG 240
           + FN +MR+VAGKRYYG+EVSDE EAREF  LMEEIS HGGAS+WVDFMPIL+WIG  GG
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240

Query: 241 FEKSLVRLTKRTDKFMQALIEE-RRNKK--VLERKN--SLLDRLLELQASEPEYYTDQII 300
           +E++L +L KRTDKFM+AL+EE R NKK   +ERKN  SLLDRLLELQ SEP+YYTDQI+
Sbjct: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300

Query: 301 KGLVLVLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSY 360
           KGLVLVLLRAGTD+++VT+NWAM QLLNNPE+L KAKAELDTK+GQDR VDE DL NL Y
Sbjct: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360

Query: 361 LQAIVSETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSF 420
           LQAIV ETLRLHP APML++HYSS+DCTVAGY IP  T LLVNAWAIHRDPKLWDDPTSF
Sbjct: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420

Query: 421 RPERFLG-AANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVD 480
           RPERFLG AANEL S KLIAFGLGRR+CPG TM  RFVGLTL L+IQCY+WKK G+E +D
Sbjct: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480

Query: 481 MGEGGGITIHKAKPLEAMCKARPAMDKLLLNA 499
           MGEGGGITI KAKPLE MCKARP MDK+ + A
Sbjct: 481 MGEGGGITILKAKPLEVMCKARPVMDKVHVKA 512

BLAST of Sgr021310 vs. NCBI nr
Match: XP_023512643.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 765.8 bits (1976), Expect = 2.4e-217
Identity = 373/502 (74.30%), Postives = 436/502 (86.85%), Query Frame = 0

Query: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
           MDF S  LLLLL  L L Q  T RRNLPPSPPS+PIIGHLHLL+RPIHRNFH IAA+YGP
Sbjct: 1   MDFLSVLLLLLLVFLFLQQFITCRRNLPPSPPSVPIIGHLHLLQRPIHRNFHHIAAKYGP 60

Query: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
           IF+LRFGSRLA+IVSSL +AEECFTKND++FANRPRLLI K+LGYN TT++T+PYGDHWR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDHWR 120

Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
           NLRRL ++EIFSTARLN++LSIRKDEI+RLL+KLHS S   F +VELK+MFSEL+FN +M
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FARVELKSMFSELSFNVVM 180

Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
           R+V GKRY+G+EVS+E EAREFR LM+E S HGGAS+W+DFMPILKWIG G +EKSL +L
Sbjct: 181 RVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWIDFMPILKWIGFGEYEKSLAKL 240

Query: 241 TKRTDKFMQALIEERRNKKVL--ERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300
           TKR DKFMQ L+E+RRN+  L  E++++LLDRLLELQASEPEYY+D+IIKG++LVLLRAG
Sbjct: 241 TKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAG 300

Query: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360
            D+++VT+ WAM QLLNNPE+++KAKAE+DTKIG+DR VDE DL NL YLQAI+SET RL
Sbjct: 301 ADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDEADLTNLHYLQAIISETFRL 360

Query: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420
           HPAAPMLL+HYSS DCTVAGYDIPRGT LLVNAWAIHRDPKLWDDP  FRPERFLG  NE
Sbjct: 361 HPAAPMLLTHYSSDDCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPMRFRPERFLGEGNE 420

Query: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480
           L S KLI FG+GRR+CPG+TMA R +GL+LGLLIQCY WK  G +KVDM EGGGITI K 
Sbjct: 421 LLSNKLIPFGMGRRACPGETMAQRLIGLSLGLLIQCYDWKNVGYQKVDMTEGGGITIQKV 480

Query: 481 KPLEAMCKARPAMDKLLLNALD 501
           +PLE +C+A P MDK+L N LD
Sbjct: 481 QPLETLCRALPIMDKVLSNGLD 499

BLAST of Sgr021310 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 1.8e-159
Identity = 278/485 (57.32%), Postives = 359/485 (74.02%), Query Frame = 0

Query: 6   AFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGPIFSLR 65
           +   L++T+ +      + +NLPP P  LPIIG+LH LK+P+H  FH ++ +YG IFSL 
Sbjct: 11  SLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLW 70

Query: 66  FGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLRRL 125
           FGSRL V+VSSL IA+ECFTKND++ ANRP  L  K++GYN TT+A SPYGDHWRNLRR+
Sbjct: 71  FGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRI 130

Query: 126 AAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIVAG 185
            +IEI S+ RLNS L IR+DEI RL+ KL   S   FT+VEL+ MFSE+ FN +MR+V+G
Sbjct: 131 LSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSG 190

Query: 186 KRYYGD--EVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKR 245
           KRYYG+  +VSD EEAR FRG+++E+   GGA++  DF+  L+W    G EK L +++KR
Sbjct: 191 KRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKR 250

Query: 246 TDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSA 305
           TD F+Q LI+E R  K     N+++D LL  Q S+PEYYTDQIIKGL++V+L AGTDTS+
Sbjct: 251 TDAFLQGLIDEHRFGK--RNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSS 310

Query: 306 VTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAP 365
           VT+ WAM+ LLN+PE++ KAK ELDT IG DR VDE D+  L YLQ+IV ETLRLH AAP
Sbjct: 311 VTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAP 370

Query: 366 MLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKK 425
           +L+ H SS D ++ GY+IP+ T L+VNAW IHRDP LW DPT F+PERF     E +  K
Sbjct: 371 LLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERF---EKEGEVNK 430

Query: 426 LIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLEA 485
           L++FGLGRR+CPG+ ++ R  GLTLGLLIQC++WK+ G+EK+DM E  GIT  K   L A
Sbjct: 431 LLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKKTSLNA 490

Query: 486 MCKAR 489
           MCK R
Sbjct: 491 MCKVR 490

BLAST of Sgr021310 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 1.8e-159
Identity = 274/489 (56.03%), Postives = 368/489 (75.26%), Query Frame = 0

Query: 8   LLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEYGPIFSLRF 67
           ++LL   L L  +  RRRNLPPSP  +LP+IGHLHL+ + +HR+ + ++ +YG +FSL+ 
Sbjct: 13  VVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFSLQL 72

Query: 68  GSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLRRLA 127
           G+RL ++VSS   AEECFTKND++FANRP  ++ K++GYN TTM  SPYG+HWRNLRRLA
Sbjct: 73  GNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLRRLA 132

Query: 128 AIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIVAGK 187
           A+EIFS   LN  LSIR+DE+++LLL L+  S  +F KVE+K+  SEL+FN  MR+VAGK
Sbjct: 133 AVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMVAGK 192

Query: 188 RYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKRTDK 247
           RY+G +V D +EA+ FR L+ E+  H GAS+  DF+P L+WI    +EK + ++++  D 
Sbjct: 193 RYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSKISQEMDA 252

Query: 248 FMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSAVTL 307
           F+Q LI E R  K      +++D LL LQ S+PEYYTDQIIKG+++VLL AGTDTSAVT+
Sbjct: 253 FLQRLIHESRINK---NNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTV 312

Query: 308 NWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAPMLL 367
            WAM+ LLN+PE L KA+ E++T++G +R ++E DLP L+YL  I+SET RL PAAPML+
Sbjct: 313 EWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPAAPMLV 372

Query: 368 SHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKKLIA 427
            H SS DC V GYD+P+GT LLVNAWAIHRDP+ WD+PT F+PER  G   EL+  KL+ 
Sbjct: 373 PHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGV--ELEPSKLMP 432

Query: 428 FGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLEAMCK 487
           FG+GRRSCPG  +A R VGLTLG LIQC++WK+ G+ K+DM EG G+T+ KA+PLEA+CK
Sbjct: 433 FGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCK 492

Query: 488 ARPAMDKLL 496
            R  + K++
Sbjct: 493 PRNILHKVV 495

BLAST of Sgr021310 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.4e-148
Identity = 261/488 (53.48%), Postives = 354/488 (72.54%), Query Frame = 0

Query: 8   LLLLLTVLILLQIRTRR-RNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGPIFSLRF 67
           L  ++T+ ILL+I +RR +NLPP PP++PIIG+LH LK P+HR F  ++  YG IFSL F
Sbjct: 10  LSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWF 69

Query: 68  GSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLRRLA 127
           GSRL V+VSS  +A ECFTKND+I ANRPR L  K++ YN TT+ ++ YGDHWRNLRR+ 
Sbjct: 70  GSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRIT 129

Query: 128 AIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGE---FTKVELKTMFSELAFNALMRIV 187
            I++ S  RLNS L +R+DE  RL+ KL      E   FTKVEL+   +E+ FNA+MR++
Sbjct: 130 TIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMI 189

Query: 188 AGKRYYGD--EVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLT 247
           +GKRYYGD  +VSD EEA++FR ++ E+    GA++  DF+P+L+ +     EK   R+ 
Sbjct: 190 SGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIA 249

Query: 248 KRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDT 307
           KR++ F++ LIEE R   +     +++D LL+L  S+PEYY+D +IKGL+  +L AGTDT
Sbjct: 250 KRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDT 309

Query: 308 SAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPA 367
           SAVT+ W M++LLN+PE+L KAK ELDT+IG+++ VDE DL  L YLQ I+SETLRLHP 
Sbjct: 310 SAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPP 369

Query: 368 APMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQS 427
           AP+LL HYSS DCT+  +++P+ T +L N W IHRDPK W+D  SF+PERF     E + 
Sbjct: 370 APLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF---EKEEEV 429

Query: 428 KKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPL 487
            K++AFGLGRR+CPG ++A R VG T+GLLIQC++W++  +EK+DM EG GIT+    PL
Sbjct: 430 NKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMKIPL 489

Query: 488 EAMCKARP 490
            AMCKA P
Sbjct: 490 RAMCKALP 494

BLAST of Sgr021310 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 526.6 bits (1355), Expect = 3.2e-148
Identity = 254/494 (51.42%), Postives = 361/494 (73.08%), Query Frame = 0

Query: 4   FSAFLLLLLTVLILLQIRTRR-RNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGPIF 63
           +S F L L  +  ++ IR R+ +NLPP PPSLPIIG+LH LKRP+HR F  ++ +YG +F
Sbjct: 9   YSVFYLALFFIFNIV-IRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVF 68

Query: 64  SLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNL 123
           SL FGSRL V+VSS    ++CFTKND++ ANRPR L  K++ YN TT+ ++ YG+HWRNL
Sbjct: 69  SLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNL 128

Query: 124 RRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRI 183
           RR+ A+++ S  R+NS   IR+DE QRL+ +L   SS  F ++EL +   ++ FN +MR+
Sbjct: 129 RRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRM 188

Query: 184 VAGKRYYGD--EVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 243
           ++GKRYYG+  + SD +EA +FR ++ E+    GA++  DFMP+L+++     EK L  +
Sbjct: 189 ISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDI 248

Query: 244 TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD 303
           + +TD F++ LIEE R KK  ER N+++D LL LQ S+PEYYTDQIIKGL L +L AGTD
Sbjct: 249 SGKTDAFLRGLIEEHRTKK--ERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTD 308

Query: 304 TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP 363
           +SAVTL W+M+ LLN+PE+L K K ELDT +GQDR VDE DLP L+YL+ +++ETLRL+ 
Sbjct: 309 SSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYT 368

Query: 364 AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ 423
            AP+LL H +S +C + GY +P+ T +L+NAWAIHRDP+LW + T+F+PERF     + +
Sbjct: 369 PAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERF---EKKGE 428

Query: 424 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP 483
            +KLIAFG+GRR+CPG+ +A+R + +TL LLIQC+ WK    +K+D+ E  G T+ K  P
Sbjct: 429 LEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVP 488

Query: 484 LEAMCKARPAMDKL 495
           L+AMCK+RP ++K+
Sbjct: 489 LKAMCKSRPVINKV 496

BLAST of Sgr021310 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 6.0e-147
Identity = 263/493 (53.35%), Postives = 353/493 (71.60%), Query Frame = 0

Query: 7   FLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEY--GPIFS 66
           F  + L++ +L   R R+ NLPPSP    PIIGHLHLLK P+HR F  ++       IFS
Sbjct: 19  FFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFS 78

Query: 67  LRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLR 126
           L  GSRL  +VSS  +AEECFTKND++ ANRP  L+ KH+GYN TTM  + YGD WRNLR
Sbjct: 79  LSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLR 138

Query: 127 RLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIV 186
           R+  IEIFS+ RLNS +SIR+DEI+RL++ L   S   F KVE+K +F  L  N ++R+V
Sbjct: 139 RIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRMV 198

Query: 187 AGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKR 246
           AGKR+YGD   ++ EA+  R L+ E+ + GGA +  D+ PIL+++    +EK + +L  R
Sbjct: 199 AGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEKHVKKLAGR 258

Query: 247 TDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSA 306
            D+F+Q+L+ E+R +KV  + N+++D LL LQ ++P+YYTD IIKG++LV++ AGTDTSA
Sbjct: 259 VDEFLQSLVNEKRVEKV--KGNTMIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSA 318

Query: 307 VTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAP 366
            TL WAM+ LLN+PE+L KAK E+D +IG DR V+E D+  L YLQ IVSETLRL+P AP
Sbjct: 319 GTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAP 378

Query: 367 MLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKK 426
           MLL H +S DC V GYD+PRGT +LVNAWAIHRDPKLW++P  F+PERF     + + KK
Sbjct: 379 MLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERF---EKKGEDKK 438

Query: 427 LIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEG-GGITIHKAKPLE 486
           L+ FG+GRRSCPG  +A R V L LG L+QC++W++  ++ +DM E   G T+ KA  L+
Sbjct: 439 LMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQ 498

Query: 487 AMCKARPAMDKLL 496
           AMCKARP + K+L
Sbjct: 499 AMCKARPIVHKVL 504

BLAST of Sgr021310 vs. ExPASy TrEMBL
Match: K7NC01 (Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1)

HSP 1 Score: 997.3 bits (2577), Expect = 2.3e-287
Identity = 501/502 (99.80%), Postives = 501/502 (99.80%), Query Frame = 0

Query: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
           MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP
Sbjct: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60

Query: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
           IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR
Sbjct: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120

Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
           NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM
Sbjct: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180

Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
           RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL
Sbjct: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240

Query: 241 TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD 300
           TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD
Sbjct: 241 TKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTD 300

Query: 301 TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP 360
           TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP
Sbjct: 301 TSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHP 360

Query: 361 AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ 420
           AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ
Sbjct: 361 AAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQ 420

Query: 421 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP 480
           SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP
Sbjct: 421 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKP 480

Query: 481 LEAMCKARPAMDKLLLNALDKI 503
           LEAMCKARPAM KLLLNALDKI
Sbjct: 481 LEAMCKARPAMYKLLLNALDKI 502

BLAST of Sgr021310 vs. ExPASy TrEMBL
Match: A0A6J1FUQ9 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 4.7e-219
Identity = 375/502 (74.70%), Postives = 437/502 (87.05%), Query Frame = 0

Query: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
           MDF    LLLLL  L L Q  TRRRNLPPSPPS PIIGHLHLL+RPIHRNFH IAA+YGP
Sbjct: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60

Query: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
           IF+LRFGSRLA+IVSSL +AEECFTKND++FANRPRLLI K+LGYN TT++T+P+GDHWR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120

Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
           NLRRL ++EIFSTARLN++LSIRKDEI+RLL+KLHS S   F KVELK+MFSEL+FN +M
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVM 180

Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
           R+V GKRY+G+EVS+E EAREFR LM+E S HGGAS+WVDFMPILKWIG G +EKSL +L
Sbjct: 181 RVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKL 240

Query: 241 TKRTDKFMQALIEERRNKKVL--ERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300
           TKR DKFMQ L+E+RRN+  L  E++++LLDRLLELQASEPEYY+D+IIKG++LVLLRAG
Sbjct: 241 TKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAG 300

Query: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360
            D+++VT+ WAM QLLNNPE+++KAKAE+DTKIG+DR +DE DL NL YLQAI+SET RL
Sbjct: 301 ADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRL 360

Query: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420
           HPAAPMLL+HYSS DCTVAGYD+PRGT LLVNAWAIHRDPKLWDDP SFRPERFLG  NE
Sbjct: 361 HPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNE 420

Query: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480
           L   KLI FG+GRR+CPG+TMALR +GL+LGLLIQCY+WK  G EKVDM EGGGITI K 
Sbjct: 421 LLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKV 480

Query: 481 KPLEAMCKARPAMDKLLLNALD 501
           +PLE MC+A P MDK+L N LD
Sbjct: 481 QPLETMCRALPIMDKVLSNGLD 499

BLAST of Sgr021310 vs. ExPASy TrEMBL
Match: A0A6J1J5B5 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.9e-218
Identity = 373/502 (74.30%), Postives = 439/502 (87.45%), Query Frame = 0

Query: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60
           MDF S FLLLLL  L L Q  TRRRNLPPSPPS+PIIGHLHLL+RPIH+NFH IAA+YGP
Sbjct: 1   MDFLSVFLLLLLVFLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAKYGP 60

Query: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120
           IF+LRFGSRLA+IVSSL +AEECFTKND++FANRPRLLI K+LGYN TT++T+PYGD WR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDLWR 120

Query: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180
           NLRRL ++E+FSTARLN++LSIRKDEI+RLL+KLHS S   F KVELK+MFSEL+FN +M
Sbjct: 121 NLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVM 180

Query: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240
           R+V GKRY+G+EVS+E EAREFR LM+EIS HGGAS+W++FMPILKWIG G +EKSL +L
Sbjct: 181 RVVTGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPILKWIGFGEYEKSLAKL 240

Query: 241 TKRTDKFMQALIEERRNKK--VLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300
           TKR DKFMQ L+E RRN+    +E++++LLDRLLELQASEPEYY+D+IIKG+VLVLLRAG
Sbjct: 241 TKRADKFMQKLVEYRRNQTGFEIEKQSTLLDRLLELQASEPEYYSDEIIKGVVLVLLRAG 300

Query: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360
            D+++VT+ WAM QLLNNPE+++KAKAE+DTKIG+DR VDE DL NL YLQAI+ ET RL
Sbjct: 301 VDSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETFRL 360

Query: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420
           HPAAPMLL+HYSS DCTVAGYDIPRGT L+VNAWAIHRDPKLWDDP SFRPERFLGA NE
Sbjct: 361 HPAAPMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAGNE 420

Query: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480
           L S KL+ FG+GRR+CPG+TMA R +GL+LGLLIQCY+WK  G +KVDM EGGGITI K 
Sbjct: 421 LLSNKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQKV 480

Query: 481 KPLEAMCKARPAMDKLLLNALD 501
           +PLE MC+A P MDK+L N LD
Sbjct: 481 QPLETMCRALPIMDKVLSNGLD 499

BLAST of Sgr021310 vs. ExPASy TrEMBL
Match: A0A6J1D806 (cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 1.1e-217
Identity = 389/512 (75.98%), Postives = 436/512 (85.16%), Query Frame = 0

Query: 1   MDFFSAFLLLLL---TVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAE 60
           MD  S F LLL    ++L   QIR  RRNLPPSPPS+PIIGHL LL+RP+HRNF KIAAE
Sbjct: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60

Query: 61  YGPIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGD 120
           YGPIFSLRFGSRLAV+VSSLD AEEC TKND++FANRPRL+I KHLGYN TTMA +PYG 
Sbjct: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120

Query: 121 HWRNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLH----SGSSGEFTKVELKTMFSE 180
           HWRNLRRL A E+FSTARL SSLSIRK+EI+R+LLKL     SGS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180

Query: 181 LAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIG-GGG 240
           + FN +MR+VAGKRYYG+EVSDE EAREF  LMEEIS HGGAS+WVDFMPIL+WIG  GG
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240

Query: 241 FEKSLVRLTKRTDKFMQALIEE-RRNKK--VLERKN--SLLDRLLELQASEPEYYTDQII 300
           +E++L +L KRTDKFM+AL+EE R NKK   +ERKN  SLLDRLLELQ SEP+YYTDQI+
Sbjct: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300

Query: 301 KGLVLVLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSY 360
           KGLVLVLLRAGTD+++VT+NWAM QLLNNPE+L KAKAELDTK+GQDR VDE DL NL Y
Sbjct: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360

Query: 361 LQAIVSETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSF 420
           LQAIV ETLRLHP APML++HYSS+DCTVAGY IP  T LLVNAWAIHRDPKLWDDPTSF
Sbjct: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420

Query: 421 RPERFLG-AANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVD 480
           RPERFLG AANEL S KLIAFGLGRR+CPG TM  RFVGLTL L+IQCY+WKK G+E +D
Sbjct: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480

Query: 481 MGEGGGITIHKAKPLEAMCKARPAMDKLLLNA 499
           MGEGGGITI KAKPLE MCKARP MDK+ + A
Sbjct: 481 MGEGGGITILKAKPLEVMCKARPVMDKVHVKA 512

BLAST of Sgr021310 vs. ExPASy TrEMBL
Match: A0A6J1D8F1 (cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 4.8e-216
Identity = 389/515 (75.53%), Postives = 436/515 (84.66%), Query Frame = 0

Query: 1   MDFFSAFLLLLL---TVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAE 60
           MD  S F LLL    ++L   QIR  RRNLPPSPPS+PIIGHL LL+RP+HRNF KIAAE
Sbjct: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60

Query: 61  YGPIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGD 120
           YGPIFSLRFGSRLAV+VSSLD AEEC TKND++FANRPRL+I KHLGYN TTMA +PYG 
Sbjct: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120

Query: 121 HWRNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLH----SGSSGEFTKVELKTMFSE 180
           HWRNLRRL A E+FSTARL SSLSIRK+EI+R+LLKL     SGS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180

Query: 181 LAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIG-GGG 240
           + FN +MR+VAGKRYYG+EVSDE EAREF  LMEEIS HGGAS+WVDFMPIL+WIG  GG
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240

Query: 241 FEKSLVRLTKRTDKFMQALIEE-RRNKK--VLERKN--SLLDRLLELQASEPEYYTDQII 300
           +E++L +L KRTDKFM+AL+EE R NKK   +ERKN  SLLDRLLELQ SEP+YYTDQI+
Sbjct: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300

Query: 301 KGLVL---VLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPN 360
           KGLVL   VLLRAGTD+++VT+NWAM QLLNNPE+L KAKAELDTK+GQDR VDE DL N
Sbjct: 301 KGLVLNMQVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN 360

Query: 361 LSYLQAIVSETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDP 420
           L YLQAIV ETLRLHP APML++HYSS+DCTVAGY IP  T LLVNAWAIHRDPKLWDDP
Sbjct: 361 LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP 420

Query: 421 TSFRPERFLG-AANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDE 480
           TSFRPERFLG AANEL S KLIAFGLGRR+CPG TM  RFVGLTL L+IQCY+WKK G+E
Sbjct: 421 TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE 480

Query: 481 KVDMGEGGGITIHKAKPLEAMCKARPAMDKLLLNA 499
            +DMGEGGGITI KAKPLE MCKARP MDK+ + A
Sbjct: 481 NIDMGEGGGITILKAKPLEVMCKARPVMDKVHVKA 515

BLAST of Sgr021310 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 549.7 bits (1415), Expect = 2.5e-156
Identity = 274/492 (55.69%), Postives = 368/492 (74.80%), Query Frame = 0

Query: 6   AFLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEY--GPIF 65
           + L ++L+++ L+    R+ NLPPSP  SLP+IGHL LLK PIHR F  ++      PIF
Sbjct: 9   SILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIF 68

Query: 66  SLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNL 125
           SLR G+RL  + SS  IAEECFTKND++ ANRP  +++KH+ Y+ TTM  + YGDHWRNL
Sbjct: 69  SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 128

Query: 126 RRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRI 185
           RR+ ++EIFS  RLNS LSIRKDEI+RL+ +L    S EF KV++K+M S+L FN ++R+
Sbjct: 129 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRM 188

Query: 186 VAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTK 245
           VAGKRYYGD V D+ EA+  R L+ ++    GA + VD++P+L+ +    +E  + +L  
Sbjct: 189 VAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLAG 248

Query: 246 RTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTS 305
           R D+F+Q L++E+R  K  E+ N+++D LL LQ S+P+Y+TD+IIKG +L L+ AGTDTS
Sbjct: 249 RLDEFLQGLVDEKREAK--EKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTS 308

Query: 306 AVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAA 365
           AVTL WA++ +LN+P++L KA+ E+D KIG DR +DE D+ NL YLQ IVSETLRL+PAA
Sbjct: 309 AVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAA 368

Query: 366 PMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSK 425
           PMLL H +S DC VAGYD+PRGT LL N WAIHRDP+LWDDP SF+PERF     E +++
Sbjct: 369 PMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERF---EKEGEAQ 428

Query: 426 KLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLE 485
           KL+ FGLGRR+CPG  +A R + LTLG LIQC +W+K G+E VDM EG G+T+ KAKPLE
Sbjct: 429 KLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLE 488

Query: 486 AMCKARPAMDKL 495
           AMC+ARP++ K+
Sbjct: 489 AMCRARPSVVKI 492

BLAST of Sgr021310 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 540.8 bits (1392), Expect = 1.2e-153
Identity = 273/493 (55.38%), Postives = 362/493 (73.43%), Query Frame = 0

Query: 7   FLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEYG--PIFS 66
           FL + L++  ++    RR NLPPSP  +LP+IGHL LLK P+HR F  ++   G  PI S
Sbjct: 10  FLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIIS 69

Query: 67  LRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLR 126
           LR G+RL  +VSS  +AEECFTKND++ ANR   L SKH+ Y CTT+ T+ YGDHWRNLR
Sbjct: 70  LRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLR 129

Query: 127 RLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIV 186
           R+ A+EIFS  RLNS  SIR+DEI RL+  L   SS EFTKVE+K+MFS L FN ++R++
Sbjct: 130 RIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRML 189

Query: 187 AGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKR 246
           AGK YYGD   D+ EA+  R L+ E     GA +  D++PIL WI G   EK + ++  R
Sbjct: 190 AGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWITGS--EKRIKKIASR 249

Query: 247 TDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSA 306
            D+F+Q L++ERR  K  +R+N+++D LL LQ ++PEYYTD IIKG++L L+ AGTDTSA
Sbjct: 250 LDEFLQGLVDERREGKE-KRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSA 309

Query: 307 VTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAP 366
           VTL W ++ LLN+P++L+KA+ E+D K+G +R V+E DL +L YLQ IVSE+LRL+PA+P
Sbjct: 310 VTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASP 369

Query: 367 MLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKK 426
           +L+ H +S DC V GY +PRGT LL NAWAIHRDPK+WDDPTSF+PERF     E +++K
Sbjct: 370 LLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERF---EKEGEAQK 429

Query: 427 LIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGE-GGGITIHKAKPLE 486
           L+ FGLGRR+CPG  +A R   LT+G LIQC++W++ G+E+VDM E GGG+ + KA PL 
Sbjct: 430 LLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLV 489

Query: 487 AMCKARPAMDKLL 496
           AMCKARP + K+L
Sbjct: 490 AMCKARPVVGKIL 496

BLAST of Sgr021310 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 530.0 bits (1364), Expect = 2.0e-150
Identity = 269/492 (54.67%), Postives = 360/492 (73.17%), Query Frame = 0

Query: 7   FLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEYG--PIFS 66
           F  ++L+++ L+    R+ NLPPSP  +LP+IGHL LLK P+HR F  ++   G  PI S
Sbjct: 10  FCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIIS 69

Query: 67  LRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNLR 126
           LR G+RL  +VSS  IAEECFTKND+I ANR   + +KH+ Y  +T+ ++ Y +HWRNLR
Sbjct: 70  LRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLR 129

Query: 127 RLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRIV 186
           R+ A+EIFS  RLNS  SIR+DEI+RL+ +L   SS  FTKVE+K+MFS+L FN ++R++
Sbjct: 130 RIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIRML 189

Query: 187 AGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKR 246
           AGK YYGD   D+ EA+  R L+ E     G  +  D++PIL WI     E  + +L  R
Sbjct: 190 AGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRIKKLAGR 249

Query: 247 TDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTSA 306
            D+F+Q L++E+R  K  +++N+++D LL LQ ++PEYY D+IIKG +L L+  GTDT+A
Sbjct: 250 LDEFLQGLVDEKREGKE-KKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTA 309

Query: 307 VTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAP 366
           VTL WA++ LLNNPE+L KA+ E+D  IG DR ++E D+PNL YLQ IVSETLRL+PAAP
Sbjct: 310 VTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAP 369

Query: 367 MLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKK 426
           MLL H +S DC V GYD+PRGT LL NAWAIHRDP LWDDPTSF+PERF     E ++KK
Sbjct: 370 MLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERF---EKEGEAKK 429

Query: 427 LIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLEA 486
           L+ FGLGRR+CPG  +A R V L+LG LIQC++W++ G+E+VDM EG G+T+ KA+PLEA
Sbjct: 430 LMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEA 489

Query: 487 MCKARPAMDKLL 496
           MC+AR  + K+L
Sbjct: 490 MCRARDFVGKIL 495

BLAST of Sgr021310 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 530.0 bits (1364), Expect = 2.0e-150
Identity = 264/493 (53.55%), Postives = 353/493 (71.60%), Query Frame = 0

Query: 6   AFLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEYG--PIF 65
           +FL L +++ +LL    R+ NLPPSP   LP+IGHLHLLK+P+HR FH I+   G  PIF
Sbjct: 10  SFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIF 69

Query: 66  SLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNL 125
            LR G+RL  ++SS  IAEECFTKND++ ANRP ++++KH+GYN T M  + YGDHWRNL
Sbjct: 70  HLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNL 129

Query: 126 RRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRI 185
           RR+AA+EIFS+ R+++  SIRKDEI+RL+  L   S   F +VELK++ + LAFN ++ +
Sbjct: 130 RRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIMM 189

Query: 186 VAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTK 245
           VAGKRYYG    D +EA+  R L+ EI    G+ +  D++P + W+    FE     L  
Sbjct: 190 VAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKILGN 249

Query: 246 RTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTS 305
           R D+ +Q L++E+R +K  E+  +L+D LL  Q +EPEYYTD IIKG++L L+ AGTDTS
Sbjct: 250 RLDRVLQKLVDEKRAEK--EKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAGTDTS 309

Query: 306 AVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAA 365
           +VTL WAM+ LLN+PE+L KA+AE+D KIG DR V+E D+ NL YLQ IVSETLRL+PA 
Sbjct: 310 SVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPAV 369

Query: 366 PMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSK 425
           P+LL H+SS +C VAGYD+PR T LL N WA+HRDP LW++P  F+PERF     E +++
Sbjct: 370 PLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERF---EKEGEAR 429

Query: 426 KLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLE 485
           KL+ FG+GRR+CPG  +  R V L LG LIQ ++W++ G E VDM EG GIT+ KA PL 
Sbjct: 430 KLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPKATPLR 489

Query: 486 AMCKARPAMDKLL 496
           AMCKAR  + K +
Sbjct: 490 AMCKARAIVGKTI 495

BLAST of Sgr021310 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 526.6 bits (1355), Expect = 2.3e-149
Identity = 262/493 (53.14%), Postives = 355/493 (72.01%), Query Frame = 0

Query: 6   AFLLLLLTVLILLQIRTRRRNLPPSPP-SLPIIGHLHLLKRPIHRNFHKIAAEYG--PIF 65
           +FL L    + LL    R+ NLPPSP  SLP+IGHLHLLK P+HR F  ++   G  P+F
Sbjct: 10  SFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVF 69

Query: 66  SLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWRNL 125
            LR G+RL  ++SS  IAEECFTKND++ ANRP+  ISKHLGYN T + ++ YGDHWRNL
Sbjct: 70  HLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNL 129

Query: 126 RRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALMRI 185
           RR+AA+EIFST RLNS L IRKDEI+RL+  L   S   F +VE+KT+ + LA N  +R+
Sbjct: 130 RRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTTIRM 189

Query: 186 VAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRLTK 245
           +AGKRY+G+   D ++A+  + L+ E     GA + +D++ IL+W+    +EK +  L  
Sbjct: 190 LAGKRYFGE---DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIKNLGN 249

Query: 246 RTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAGTDTS 305
           R D F+Q L++E+R +K  E+  +++D LL LQ  +P+YYTD IIKG++L L+ AGTDTS
Sbjct: 250 RFDTFLQKLVDEKRAEK--EKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAGTDTS 309

Query: 306 AVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAA 365
           +VTL WAM+ LLN+PE+L KA+ E+D K+G DR VDE D+ NLSYLQ+IV ETLR++PA 
Sbjct: 310 SVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMYPAV 369

Query: 366 PMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSK 425
           P+LL H SS DC V GYDIP GT +L NAWA+HRDP++W+DP  F+PERF     E +++
Sbjct: 370 PLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERF---EKEGEAE 429

Query: 426 KLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLE 485
           KLI+FG+GRR+CPG  +A R +   LG L+QC++W++ G++ VDM E  G T+ KA PL 
Sbjct: 430 KLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAIPLR 489

Query: 486 AMCKARPAMDKLL 496
           AMCKAR  +DKL+
Sbjct: 490 AMCKARSIVDKLI 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AEM42992.14.8e-28799.80cytochrome P450 [Siraitia grosvenorii][more]
XP_022944057.19.6e-21974.70cytochrome P450 81E8-like [Cucurbita moschata] >KAG7011030.1 Cytochrome P450 81E... [more]
XP_022985607.18.1e-21874.30cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E... [more]
XP_022149457.12.4e-21775.98cytochrome P450 81E8-like isoform X2 [Momordica charantia][more]
XP_023512643.12.4e-21774.30cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q6WNQ81.8e-15957.32Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
W8JMU71.8e-15956.03Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q6WNQ91.4e-14853.48Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
P931473.2e-14851.42Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Q9LHA16.0e-14753.35Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
K7NC012.3e-28799.80Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1[more]
A0A6J1FUQ94.7e-21974.70cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=... [more]
A0A6J1J5B53.9e-21874.30cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1[more]
A0A6J1D8061.1e-21775.98cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1D8F14.8e-21675.53cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
Match NameE-valueIdentityDescription
AT4G37370.12.5e-15655.69cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37340.11.2e-15355.38cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
AT4G37360.12.0e-15054.67cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT4G37320.12.0e-15053.55cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
AT4G37330.12.3e-14953.14cytochrome P450, family 81, subfamily D, polypeptide 4 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 350..361
score: 42.16
coord: 297..314
score: 39.21
coord: 425..434
score: 52.6
IPR001128Cytochrome P450PFAMPF00067p450coord: 28..475
e-value: 6.5E-97
score: 325.2
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 434..457
score: 28.32
coord: 306..332
score: 36.67
coord: 390..414
score: 42.58
coord: 55..74
score: 35.85
coord: 79..100
score: 23.7
coord: 424..434
score: 50.04
coord: 286..303
score: 33.29
coord: 349..367
score: 33.67
coord: 174..192
score: 20.99
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 20..498
e-value: 8.6E-126
score: 422.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 28..488
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 8..453
e-value: 8.2E-17
score: 59.1
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 6..493
NoneNo IPR availablePANTHERPTHR24298:SF836CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATEDcoord: 6..493
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 427..436

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr021310.1Sgr021310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen