Sgr021308 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr021308
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Cytochrome P450
Location	tig00153654: 820820 .. 825003 (+)
RNA-Seq Expression	Sgr021308
Synteny	Sgr021308

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTTTCTGATCCTTGCTTTCCTTCTGCTTCTTCTTGCCATCCTTTTTCTCCAACAGCTCAGAACCCGTCGGAGAAATCTGCCGCCGTGCCCGCCGTCGCTTCCGATCATCGGCCACCTCCACCTCCTCAAACGCCCCACCCATCGTAATTTTCACAAGCTTGCCGCCAAATACGGTCCCATTTTCTCTCTCAGATTTGGCCCTCGCCTTGCTGTCATAGTTTCTTCCCTTGATATAGCTGAAGAATGTTTCACCAAGAACGACATCGTTTTTGCCGACCGGCCACGTCTGCTCGTCGGCAAATACCTTGGCTACAACTACACCACCATAGCCACCGCGCCTCACGGGGACCACTGGCGGAACCTCCGCCGCCTTGCGGCCGTCGAGATCTTCTCGACGGCTCGGCTGAACTCCACTCTGAGCATCCGTAAGGACGAAATCCAGCGCATGTTACTCAAACTCCGTACCGGATCGAGCGGAGAGTTCACCAAGGTTGAACTGAAAACGATGTTTTCGGAGCTGACTTTCAACGCCGTGATGAGGATCGTGGCCGGGAAACGGTATCACGGCGACGAGGTTTCTGATGAGGAGGAGGCGAGGGAGTTCAGAGAGCTTATGGTGGAGATTTCAGCTCATGGAGGAGCATCGCATCGGGTTGATTACCTGCCGATTCTTCTGAAATGGATTGTCGGCGACGGATTTGAACAGAGTTTGGTAAGGCTGAGGAAGAGGACAGATAAATTCATGCAGGCACTGATTGAGGAACGCCGCAACAAGAAAGCGATAGAAAGAAAGAATTCTCTGCTTGATCGTCTTCTGGAATTGCAGGCTGCAGAACCACAATATTACACCGATCAGATCATCAAAGGCCTCATTCTGGTTAGTATTCCAACTCCACCGCTTGTTACTTCTCTCTCAAAATCTTAATCAATCCATGCCTTTTTTGCTTAGTAACTAAAATTTCCCTCTAGATCTATCTTTGTTCTCTAAGTTTTCAAAAATTCGTGGGTTTTAAAAAATGATGACATTTTAAACTTTTTTATTTAAGAACAATGTTTGTTTATCCTGCTACCCATGATTTTCAAAAATTAGAAAAAATCTCTCTTTTCTGTTTTCTCTCTACCTATCTGTCAATAATTTAATGATAGGTAGAAAGAGGCTAAGTATTATCCTTTATTAATATTCAAAAGTTAAAATGTAATTTTTAAAAATTTACGAATCCAGGTGACTTTTAAATGAAAACTATAAGACTGAAAAAGTAATTTTTTCCTTATTAGTTCTGTTTGTTGAATTTTGTTAAAGGGAGAATGATATAGACTTGGGGCGTGCAGGGACTAGCGAGTGGTTTAACAATATATGCTTTTATCAAAATTATTAATTATTCATAACTACTTCAATAAAAGGTGTGCCATAATAAGATAATGGGAAAAAAAATTTGGTAGGGTGAGTACATTTCTACCTAAGCTTTCAATTTTATGAATTCCGACCATAAATTTGAATAAATATTATAATTTTTGTTGAAATTGATAAGTTTTGAGACATTTATTCAATGTTCCATCACCATGTGCATGAATTGGCTAGGACTTGATTTTATCTATGGATTTGCTACGGCTCGATTTTTGCTGAAATTAGTGGATTTTGAGAAATTTCTATACTTTTTTTTTGTTGACATTAGTGGGTAAATGTAACAGAAGCCTAGAAGATAAAAATTGTAAAATTTATCCAAAGTCTAAAATTCAATTTGATGAAATGAAAAGTTTATAGTAAAAATTGACTCACCTTGCCAAGTTTTGGGTACGAATCGAACTTTTCTTTTAAATATTTTTATGGCTTGGATTTAATAATTCAAAAGTACTTTATTTCATTAAAAATATTTTTTTAATGATTAATTAATATTTACTAAATAAATATTTCTAGAATTGACAAATCGTTGAAATGAAAATAATTAAAATTATGAAAATTACTAAAATTGAAACAGTTAAAATTTTAAAAATGAAATAAGTGAAAGAAAATGTGTGTATAAAAAAATTAGTGGAAAAAAAAATCGTATGTGCACAGAGAAATTAGTAAAATTAAAGAAATTGTGTGTGAAGGAAGAGATTAACGAGAGAAAGTATACATATATTAAGAGAAATTAGTGAGAGAAAGTATATGTTGAGAGAAATTGGTAGGTGAAAATATGTGAATGAGAAGTTAGTTAAAGAAAATGTGCTTGAAGATAGAAATTAGAGAGAAAATGTGTGTAGACAAAAAATTAGTGTAGCGAGAGAAACTATATATCAAAAGTAACGAGAGAAATGTGTCTCTTGTATCAATTTTAGAATGCTAAAAGTTTTAACTAATTTAAAACATTTTTCAATATATATATATTTTTTGATGTTTGCCTTATAGCGAAAATTGATTTTTCAAAATTCTAGAAACAATTAATTAGTGCTCTTCAAAAATATTATAAAAAAAAATATTTACTTAAAAACACTGTTTTCAAAAGTCATAAATTTATAGAAGAATACTTTTAAGTGCTTTTAAGTTTTTAAAAAGTGCATATAAATTGTCGTAGATTTGAGGTATGAAGTAGTTTTCATAATTTAAATACAGAAAAAGTAGACATGATTATTTTGTTAATTAAGCCATCAAAATTAATGTTCTTTCAACCAAATCAAAGTAAACGTTCTTTCAATCAGAACTTTTTACAAAAGAATGAAACCAATTAAGTTTTAAAAACCTAAAGTGAAATTATGGGGAAAAATGGCATATTAATGTGATGTAAAATATGATTGAAGTGAGCACCAATGTCTCCTCTCATGGATTATAGTTTTTAATATTTTTCTTCACATTTGTAATGTAATATATTTTTTAAAATTATTAATATGGTAAAAAAAAAGATTAGCTAAAAGTGATATTACTTATCAATTATCTTATCTTTTTTCTTGTTCATTTCAAAAAAAAAAAATTATCTTTTTTTCTTGAAGGACAATGTTCATACTTCAGAATAGAGATGACAGTTTTGCCATGAAGATAGGGCCCTAAGAGGATTCCTCGATTTAATTAGGGACGATAATTTTTTTCTCAATTTAATTTTTAGAATAGGAACAAGAATATATTTTTCATCTCAACTTCGATCCCATCTCCATTCCCATCCCTAAATTTTGAATTACTTTTATCAACTTTTATATTTCGACATTAGTAAAATGATTAAATATTTGCATGAAATTAATTATTAAAATTATAAATAAATTAGAGAATTAACTTTAAAAGGTATGTTTTTTTAATTATTTAATATTTAATTAGTCTTTAATTACATAGCCATAAACTAAAAAGTAGAGATTTTTCCCTACGAAGAATCCCATAACCGATATTGATCTCTAAAGTCCGAGACGAAGAAGAGATGGGGATGAGATTCAAATTTCCCATGGAGACGGTATGGGGAAGGCAACTCCGACCTCGACCTGCTCCATTGTGATCCCTAGTTAGGAAGATGCTTAAATGATTCTTACTCTGCTCAAGTCACAGGGGATTTGATTTTCCTTTAATGAAGTAGAAATGTGCAGGTTTTACTATTTGCAGGAACAGACTCATCATCTGCAACCTTGGAATGGGCAATGACCGAACTGCTCAGAAAAACAAAAGTACTAACCAAAGCCAAAGCCGAGTTGGACACAGAGATCGGTCAAGATCGACCAATTGACGAAGCAGATCTCACCAACCTCAACTATCTACAAGCCATTGTTTCCGAGACCCTCCGCTTGCACCCCGCTGCTCCGATGCTCCTCCCACATTACTCCTCCGACGATTGCACCGTCGCCGGCTACAGTATTCCACGAGACACAATGTTATTGGTCAATGCATGGGCCATCCACAGAGACCCAAGGCTGTGGGACGACCCCACGAGCTTCCGACCGGAGAGGTTTATAGGAGTGGCTAATGAGTTGCAGTCGAACAAACTTATTGCGTTTGGGCTTGGAAGGAGGGCTTGTCCTGGAGCAAGTATGGCTCAGCACTTTCTGGGTTTGACTTTAGGGTTACTGATTCAGTGTTATGAGTGGAAAAAGGTTGGAGATGAAGAGATTGACATGGCTGAAAGCGGGGGCATTACTGGTCACAAAGTTGAGCCTTTGGAAGCCATGTGTAAAGCTCGCCCAGTCATGAATAAAGTTTTGTTAAATACTTTGGATGAAATTTGA

mRNA sequence

ATGGACTTTCTGATCCTTGCTTTCCTTCTGCTTCTTCTTGCCATCCTTTTTCTCCAACAGCTCAGAACCCGTCGGAGAAATCTGCCGCCGTGCCCGCCGTCGCTTCCGATCATCGGCCACCTCCACCTCCTCAAACGCCCCACCCATCGTAATTTTCACAAGCTTGCCGCCAAATACGGTCCCATTTTCTCTCTCAGATTTGGCCCTCGCCTTGCTGTCATAGTTTCTTCCCTTGATATAGCTGAAGAATGTTTCACCAAGAACGACATCGTTTTTGCCGACCGGCCACGTCTGCTCGTCGGCAAATACCTTGGCTACAACTACACCACCATAGCCACCGCGCCTCACGGGGACCACTGGCGGAACCTCCGCCGCCTTGCGGCCGTCGAGATCTTCTCGACGGCTCGGCTGAACTCCACTCTGAGCATCCGTAAGGACGAAATCCAGCGCATGTTACTCAAACTCCGTACCGGATCGAGCGGAGAGTTCACCAAGGTTGAACTGAAAACGATGTTTTCGGAGCTGACTTTCAACGCCGTGATGAGGATCGTGGCCGGGAAACGGTATCACGGCGACGAGGTTTCTGATGAGGAGGAGGCGAGGGAGTTCAGAGAGCTTATGGTGGAGATTTCAGCTCATGGAGGAGCATCGCATCGGGTTGATTACCTGCCGATTCTTCTGAAATGGATTGTCGGCGACGGATTTGAACAGAGTTTGGTAAGGCTGAGGAAGAGGACAGATAAATTCATGCAGGCACTGATTGAGGAACGCCGCAACAAGAAAGCGATAGAAAGAAAGAATTCTCTGCTTGATCGTCTTCTGGAATTGCAGGCTGCAGAACCACAATATTACACCGATCAGATCATCAAAGGCCTCATTCTGGTTTTACTATTTGCAGGAACAGACTCATCATCTGCAACCTTGGAATGGGCAATGACCGAACTGCTCAGAAAAACAAAAGTACTAACCAAAGCCAAAGCCGAGTTGGACACAGAGATCGGTCAAGATCGACCAATTGACGAAGCAGATCTCACCAACCTCAACTATCTACAAGCCATTGTTTCCGAGACCCTCCGCTTGCACCCCGCTGCTCCGATGCTCCTCCCACATTACTCCTCCGACGATTGCACCGTCGCCGGCTACAGTATTCCACGAGACACAATGTTATTGGTCAATGCATGGGCCATCCACAGAGACCCAAGGCTGTGGGACGACCCCACGAGCTTCCGACCGGAGAGGTTTATAGGAGTGGCTAATGAGTTGCAGTCGAACAAACTTATTGCGTTTGGGCTTGGAAGGAGGGCTTGTCCTGGAGCAAGTATGGCTCAGCACTTTCTGGGTTTGACTTTAGGGTTACTGATTCAGTGTTATGAGTGGAAAAAGGTTGGAGATGAAGAGATTGACATGGCTGAAAGCGGGGGCATTACTGGTCACAAAGTTGAGCCTTTGGAAGCCATGTGTAAAGCTCGCCCAGTCATGAATAAAGTTTTGTTAAATACTTTGGATGAAATTTGA

Coding sequence (CDS)

ATGGACTTTCTGATCCTTGCTTTCCTTCTGCTTCTTCTTGCCATCCTTTTTCTCCAACAGCTCAGAACCCGTCGGAGAAATCTGCCGCCGTGCCCGCCGTCGCTTCCGATCATCGGCCACCTCCACCTCCTCAAACGCCCCACCCATCGTAATTTTCACAAGCTTGCCGCCAAATACGGTCCCATTTTCTCTCTCAGATTTGGCCCTCGCCTTGCTGTCATAGTTTCTTCCCTTGATATAGCTGAAGAATGTTTCACCAAGAACGACATCGTTTTTGCCGACCGGCCACGTCTGCTCGTCGGCAAATACCTTGGCTACAACTACACCACCATAGCCACCGCGCCTCACGGGGACCACTGGCGGAACCTCCGCCGCCTTGCGGCCGTCGAGATCTTCTCGACGGCTCGGCTGAACTCCACTCTGAGCATCCGTAAGGACGAAATCCAGCGCATGTTACTCAAACTCCGTACCGGATCGAGCGGAGAGTTCACCAAGGTTGAACTGAAAACGATGTTTTCGGAGCTGACTTTCAACGCCGTGATGAGGATCGTGGCCGGGAAACGGTATCACGGCGACGAGGTTTCTGATGAGGAGGAGGCGAGGGAGTTCAGAGAGCTTATGGTGGAGATTTCAGCTCATGGAGGAGCATCGCATCGGGTTGATTACCTGCCGATTCTTCTGAAATGGATTGTCGGCGACGGATTTGAACAGAGTTTGGTAAGGCTGAGGAAGAGGACAGATAAATTCATGCAGGCACTGATTGAGGAACGCCGCAACAAGAAAGCGATAGAAAGAAAGAATTCTCTGCTTGATCGTCTTCTGGAATTGCAGGCTGCAGAACCACAATATTACACCGATCAGATCATCAAAGGCCTCATTCTGGTTTTACTATTTGCAGGAACAGACTCATCATCTGCAACCTTGGAATGGGCAATGACCGAACTGCTCAGAAAAACAAAAGTACTAACCAAAGCCAAAGCCGAGTTGGACACAGAGATCGGTCAAGATCGACCAATTGACGAAGCAGATCTCACCAACCTCAACTATCTACAAGCCATTGTTTCCGAGACCCTCCGCTTGCACCCCGCTGCTCCGATGCTCCTCCCACATTACTCCTCCGACGATTGCACCGTCGCCGGCTACAGTATTCCACGAGACACAATGTTATTGGTCAATGCATGGGCCATCCACAGAGACCCAAGGCTGTGGGACGACCCCACGAGCTTCCGACCGGAGAGGTTTATAGGAGTGGCTAATGAGTTGCAGTCGAACAAACTTATTGCGTTTGGGCTTGGAAGGAGGGCTTGTCCTGGAGCAAGTATGGCTCAGCACTTTCTGGGTTTGACTTTAGGGTTACTGATTCAGTGTTATGAGTGGAAAAAGGTTGGAGATGAAGAGATTGACATGGCTGAAAGCGGGGGCATTACTGGTCACAAAGTTGAGCCTTTGGAAGCCATGTGTAAAGCTCGCCCAGTCATGAATAAAGTTTTGTTAAATACTTTGGATGAAATTTGA

Protein sequence

MDFLILAFLLLLLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYGPIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHWRNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAVMRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLVRLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAGTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRLHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHKVEPLEAMCKARPVMNKVLLNTLDEI

Homology

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BLAST of Sgr021308 vs. NCBI nr
Match: AEM42992.1 (cytochrome P450 [Siraitia grosvenorii])

HSP 1 Score: 824.3 bits (2128), Expect = 5.6e-235
Identity = 410/504 (81.35%), Postives = 454/504 (90.08%), Query Frame = 0


Query: 1   MDFLILAFLLLLLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYG 60
           MDF   AFLLLLL +L L Q+RTRRRNLPP PPSLPIIGHLHLLKRP HRNFHK+AA+YG
Sbjct: 1   MDF-FSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYG 60

Query: 61  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 120
           PIFSLRFG RLAVIVSSLDIAEECFTKND++FA+RPRLL+ K+LGYN TT+AT+P+GDHW
Sbjct: 61  PIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHW 120

Query: 121 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 180
           RNLRRLAA+EIFSTARLNS+LSIRKDEIQR+LLKL +GSSGEFTKVELKTMFSEL FNA+
Sbjct: 121 RNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNAL 180

Query: 181 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 240
           MRIVAGKRY+GDEVSDEEEAREFR LM EIS HGGASH VD++PI LKWI G GFE+SLV
Sbjct: 181 MRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPI-LKWIGGGGFEKSLV 240

Query: 241 RLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAG 300
           RL KRTDKFMQALIEERRNKK +ERKNSLLDRLLELQA+EP+YYTDQIIKGL+LVLL AG
Sbjct: 241 RLTKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300

Query: 301 TDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRL 360
           TD+S+ TL WAM +LL   ++L KAKAELDT+IGQDRP+DE DL NL+YLQAIVSETLRL
Sbjct: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360

Query: 361 HPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANE 420
           HPAAPMLL HYSS DCTVAGY IPR T LLVNAWAIHRDP+LWDDPTSFRPERF+G ANE
Sbjct: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420

Query: 421 LQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHKV 480
           LQS KLIAFGLGRR+CPG +MA  F+GLTLGLLIQCY+WKK GDE++DM E GGIT HK 
Sbjct: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480

Query: 481 EPLEAMCKARPVMNKVLLNTLDEI 505
           +PLEAMCKARP M K+LLN LD+I
Sbjct: 481 KPLEAMCKARPAMYKLLLNALDKI 502

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BLAST of Sgr021308 vs. NCBI nr
Match: XP_022944057.1 (cytochrome P450 81E8-like [Cucurbita moschata] >KAG7011030.1 Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 726.9 bits (1875), Expect = 1.2e-205
Identity = 362/504 (71.83%), Postives = 428/504 (84.92%), Query Frame = 0


Query: 1   MDFLILAFLLLLLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYG 60
           MDFL +  LLLLL  LFLQQ  TRRRNLPP PPS PIIGHLHLL+RP HRNFH +AAKYG
Sbjct: 1   MDFLFV-LLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYG 60

Query: 61  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 120
           PIF+LRFG RLA+IVSSL +AEECFTKND+VFA+RPRLL+GKYLGYN+TTI+T P GDHW
Sbjct: 61  PIFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHW 120

Query: 121 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 180
           RNLRRL ++EIFSTARLN+ LSIRKDEI+R+L+KL + S   F KVELK+MFSEL+FN V
Sbjct: 121 RNLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVV 180

Query: 181 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 240
           MR+V GKRY G+EVS+E EAREFRELM E S HGGAS+ VD++PI LKWI    +E+SL 
Sbjct: 181 MRVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPI-LKWIGFGEYEKSLA 240

Query: 241 RLRKRTDKFMQALIEERRNKKA--IERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLF 300
           +L KR DKFMQ L+E+RRN+    IE++++LLDRLLELQA+EP+YY+D+IIKG+ILVLL 
Sbjct: 241 KLTKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLR 300

Query: 301 AGTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETL 360
           AG DS+S T+EWAMT+LL   +V++KAKAE+DT+IG+DR IDEADL NL+YLQAI+SET 
Sbjct: 301 AGADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETF 360

Query: 361 RLHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVA 420
           RLHPAAPMLL HYSS+DCTVAGY +PR TMLLVNAWAIHRDP+LWDDP SFRPERF+G  
Sbjct: 361 RLHPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEG 420

Query: 421 NELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGH 480
           NEL  NKLI FG+GRRACPG +MA   +GL+LGLLIQCYEWK VG E++DM E GGIT  
Sbjct: 421 NELLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQ 480

Query: 481 KVEPLEAMCKARPVMNKVLLNTLD 503
           KV+PLE MC+A P+M+KVL N LD
Sbjct: 481 KVQPLETMCRALPIMDKVLSNGLD 499

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BLAST of Sgr021308 vs. NCBI nr
Match: XP_023512643.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 726.5 bits (1874), Expect = 1.6e-205
Identity = 359/504 (71.23%), Postives = 431/504 (85.52%), Query Frame = 0


Query: 1   MDFLILAFLLLLLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYG 60
           MDFL +  LLLLL  LFLQQ  T RRNLPP PPS+PIIGHLHLL+RP HRNFH +AAKYG
Sbjct: 1   MDFLSV-LLLLLLVFLFLQQFITCRRNLPPSPPSVPIIGHLHLLQRPIHRNFHHIAAKYG 60

Query: 61  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 120
           PIF+LRFG RLA+IVSSL +AEECFTKND+VFA+RPRLL+GKYLGYN+TTI+T P+GDHW
Sbjct: 61  PIFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDHW 120

Query: 121 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 180
           RNLRRL ++EIFSTARLN+ LSIRKDEI+R+L+KL + S   F +VELK+MFSEL+FN V
Sbjct: 121 RNLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FARVELKSMFSELSFNVV 180

Query: 181 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 240
           MR+V GKRY G+EVS+E EAREFRELM E S HGGAS+ +D++PI LKWI    +E+SL 
Sbjct: 181 MRVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWIDFMPI-LKWIGFGEYEKSLA 240

Query: 241 RLRKRTDKFMQALIEERRNKKA--IERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLF 300
           +L KR DKFMQ L+E+RRN+    IE++++LLDRLLELQA+EP+YY+D+IIKG+ILVLL 
Sbjct: 241 KLTKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLR 300

Query: 301 AGTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETL 360
           AG DS+S T+EWAMT+LL   +V++KAKAE+DT+IG+DR +DEADLTNL+YLQAI+SET 
Sbjct: 301 AGADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDEADLTNLHYLQAIISETF 360

Query: 361 RLHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVA 420
           RLHPAAPMLL HYSSDDCTVAGY IPR TMLLVNAWAIHRDP+LWDDP  FRPERF+G  
Sbjct: 361 RLHPAAPMLLTHYSSDDCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPMRFRPERFLGEG 420

Query: 421 NELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGH 480
           NEL SNKLI FG+GRRACPG +MAQ  +GL+LGLLIQCY+WK VG +++DM E GGIT  
Sbjct: 421 NELLSNKLIPFGMGRRACPGETMAQRLIGLSLGLLIQCYDWKNVGYQKVDMTEGGGITIQ 480

Query: 481 KVEPLEAMCKARPVMNKVLLNTLD 503
           KV+PLE +C+A P+M+KVL N LD
Sbjct: 481 KVQPLETLCRALPIMDKVLSNGLD 499

[Close]

BLAST of Sgr021308 vs. NCBI nr
Match: XP_022149457.1 (cytochrome P450 81E8-like isoform X2 [Momordica charantia])

HSP 1 Score: 724.5 bits (1869), Expect = 6.1e-205
Identity = 372/509 (73.08%), Postives = 432/509 (84.87%), Query Frame = 0


Query: 1   MDFLILAFLLL--LLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAK 60
           MD L +  LLL  L +ILF QQ+R  RRNLPP PPS+PIIGHL LL+RP HRNF K+AA+
Sbjct: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60

Query: 61  YGPIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGD 120
           YGPIFSLRFG RLAV+VSSLD AEEC TKND+VFA+RPRL++GK+LGYN+TT+A AP+G 
Sbjct: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120

Query: 121 HWRNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLR----TGSSGEFTKVELKTMFSE 180
           HWRNLRRL A E+FSTARL S+LSIRK+EI+R+LLKLR    +GS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180

Query: 181 LTFNAVMRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGD- 240
           +TFN VMR+VAGKRY+G+EVSDE EAREF ELM EIS HGGAS+ VD++PI L+WI  D 
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPI-LRWIGCDG 240

Query: 241 GFEQSLVRLRKRTDKFMQALIEE-RRNKK--AIERKN--SLLDRLLELQAAEPQYYTDQI 300
           G+E++L +L KRTDKFM+AL+EE R NKK  AIERKN  SLLDRLLELQ +EPQYYTDQI
Sbjct: 241 GYERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQI 300

Query: 301 IKGLILVLLFAGTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLN 360
           +KGL+LVLL AGTDS+S T+ WAMT+LL   +VLTKAKAELDT++GQDR +DE+DL NL 
Sbjct: 301 VKGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLK 360

Query: 361 YLQAIVSETLRLHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTS 420
           YLQAIV ETLRLHP APML+ HYSS DCTVAGY IP DT+LLVNAWAIHRDP+LWDDPTS
Sbjct: 361 YLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTS 420

Query: 421 FRPERFIG-VANELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEI 480
           FRPERF+G  ANEL SNKLIAFGLGRRACPGA+M + F+GLTL L+IQCYEWKK G+E I
Sbjct: 421 FRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENI 480

Query: 481 DMAESGGITGHKVEPLEAMCKARPVMNKV 497
           DM E GGIT  K +PLE MCKARPVM+KV
Sbjct: 481 DMGEGGGITILKAKPLEVMCKARPVMDKV 508

[Close]

BLAST of Sgr021308 vs. NCBI nr
Match: XP_022985607.1 (cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E8-like [Cucurbita maxima])

HSP 1 Score: 723.8 bits (1867), Expect = 1.0e-204
Identity = 356/504 (70.63%), Postives = 431/504 (85.52%), Query Frame = 0


Query: 1   MDFLILAFLLLLLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYG 60
           MDFL + FLLLLL  LFLQQ  TRRRNLPP PPS+PIIGHLHLL+RP H+NFH +AAKYG
Sbjct: 1   MDFLSV-FLLLLLVFLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAKYG 60

Query: 61  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 120
           PIF+LRFG RLA+IVSSL +AEECFTKND+VFA+RPRLL+GKYLGYN+TTI+T P+GD W
Sbjct: 61  PIFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDLW 120

Query: 121 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 180
           RNLRRL ++E+FSTARLN+ LSIRKDEI+R+L+KL + S   F KVELK+MFSEL+FN V
Sbjct: 121 RNLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVV 180

Query: 181 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 240
           MR+V GKRY G+EVS+E EAREFRELM EIS HGGAS+ ++++PI LKWI    +E+SL 
Sbjct: 181 MRVVTGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPI-LKWIGFGEYEKSLA 240

Query: 241 RLRKRTDKFMQALIEERRNKKA--IERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLF 300
           +L KR DKFMQ L+E RRN+    IE++++LLDRLLELQA+EP+YY+D+IIKG++LVLL 
Sbjct: 241 KLTKRADKFMQKLVEYRRNQTGFEIEKQSTLLDRLLELQASEPEYYSDEIIKGVVLVLLR 300

Query: 301 AGTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETL 360
           AG DS+S T+EWAMT+LL   +V++KAKAE+DT+IG+DR +DE+DL NL+YLQAI+ ET 
Sbjct: 301 AGVDSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETF 360

Query: 361 RLHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVA 420
           RLHPAAPMLL HYSSDDCTVAGY IPR TML+VNAWAIHRDP+LWDDP SFRPERF+G  
Sbjct: 361 RLHPAAPMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAG 420

Query: 421 NELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGH 480
           NEL SNKL+ FG+GRRACPG +MAQ  +GL+LGLLIQCYEWK VG +++DM E GGIT  
Sbjct: 421 NELLSNKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQ 480

Query: 481 KVEPLEAMCKARPVMNKVLLNTLD 503
           KV+PLE MC+A P+M+KVL N LD
Sbjct: 481 KVQPLETMCRALPIMDKVLSNGLD 499

[Close]

BLAST of Sgr021308 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 1.1e-156
Identity = 276/489 (56.44%), Postives = 364/489 (74.44%), Query Frame = 0


Query: 4   LILAFLLLLLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYGPIF 63
           LI++   L++ +        + +NLPP P  LPIIG+LH LK+P H  FH L+ KYG IF
Sbjct: 8   LIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIF 67

Query: 64  SLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHWRNL 123
           SL FG RL V+VSSL IA+ECFTKNDIV A+RP  L GKY+GYN TT+A +P+GDHWRNL
Sbjct: 68  SLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNL 127

Query: 124 RRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAVMRI 183
           RR+ ++EI S+ RLNS L IR+DEI R++ KL   S   FT+VEL+ MFSE+TFN +MR+
Sbjct: 128 RRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRM 187

Query: 184 VAGKRYHGD--EVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLVR 243
           V+GKRY+G+  +VSD EEAR FR ++ E+ + GGA++  D+L   L+W   DG E+ L +
Sbjct: 188 VSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLG-FLRWFDFDGLEKRLKK 247

Query: 244 LRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAGT 303
           + KRTD F+Q LI+E R  K     N+++D LL  Q ++P+YYTDQIIKGL++V+L AGT
Sbjct: 248 ISKRTDAFLQGLIDEHRFGK--RNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGT 307

Query: 304 DSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRLH 363
           D+SS T+EWAM+ LL   +++ KAK ELDT IG DR +DE D++ L YLQ+IV ETLRLH
Sbjct: 308 DTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLH 367

Query: 364 PAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANEL 423
            AAP+L+PH SS+D ++ GY+IP++T+L+VNAW IHRDP LW DPT F+PERF     E 
Sbjct: 368 AAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERF---EKEG 427

Query: 424 QSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHKVE 483
           + NKL++FGLGRRACPG +++Q   GLTLGLLIQC+EWK++G+E+IDM E+ GIT  K  
Sbjct: 428 EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKKT 487

Query: 484 PLEAMCKAR 491
            L AMCK R
Sbjct: 488 SLNAMCKVR 490

[Close]

BLAST of Sgr021308 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.6e-152
Identity = 271/499 (54.31%), Postives = 368/499 (73.75%), Query Frame = 0


Query: 3   FLILAFLLLLLAILFLQQLRTRRRNLPPCPP-SLPIIGHLHLLKRPTHRNFHKLAAKYGP 62
           +  LA +LL ++ L L  +  RRRNLPP P  +LP+IGHLHL+ +  HR+ + L+ KYG 
Sbjct: 8   YTFLAVVLLSIS-LKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGS 67

Query: 63  IFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHWR 122
           +FSL+ G RL ++VSS   AEECFTKNDIVFA+RP  ++GKY+GYNYTT+  +P+G+HWR
Sbjct: 68  VFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWR 127

Query: 123 NLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAVM 182
           NLRRLAAVEIFS   LN  LSIR+DE++++LL L   S  +F KVE+K+  SEL+FN  M
Sbjct: 128 NLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTM 187

Query: 183 RIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLVR 242
           R+VAGKRY G +V D +EA+ FR L+ E+  H GAS+  D++P  L+WI    +E+ + +
Sbjct: 188 RMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVP-FLRWIDFKNYEKKVSK 247

Query: 243 LRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAGT 302
           + +  D F+Q LI E R  K      +++D LL LQ ++P+YYTDQIIKG+I+VLL AGT
Sbjct: 248 ISQEMDAFLQRLIHESRINK---NNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGT 307

Query: 303 DSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRLH 362
           D+S+ T+EWAM+ LL   + L KA+ E++T++G +R I+E DL  L YL  I+SET RL 
Sbjct: 308 DTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLC 367

Query: 363 PAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANEL 422
           PAAPML+PH SSDDC V GY +P+ T+LLVNAWAIHRDP  WD+PT F+PER  GV  EL
Sbjct: 368 PAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGV--EL 427

Query: 423 QSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHKVE 482
           + +KL+ FG+GRR+CPG+ +AQ  +GLTLG LIQC+EWK++G+ +IDMAE  G+T  K +
Sbjct: 428 EPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQ 487

Query: 483 PLEAMCKARPVMNKVLLNT 501
           PLEA+CK R +++KV+  T
Sbjct: 488 PLEALCKPRNILHKVVSET 498

[Close]

BLAST of Sgr021308 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 5.4e-148
Identity = 260/497 (52.31%), Postives = 360/497 (72.43%), Query Frame = 0


Query: 6   LAFLLLLLAILFLQQLRTRR-RNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYGPIFS 65
           L  L  ++ I  L ++ +RR +NLPP PP++PIIG+LH LK P HR F  L+  YG IFS
Sbjct: 7   LLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 66

Query: 66  LRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHWRNLR 125
           L FG RL V+VSS  +A ECFTKNDI+ A+RPR L GKY+ YNYTT+ +A +GDHWRNLR
Sbjct: 67  LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 126

Query: 126 RLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGE---FTKVELKTMFSELTFNAVM 185
           R+  +++ S  RLNS L +R+DE  R++ KL      E   FTKVEL+   +E+TFNA+M
Sbjct: 127 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMM 186

Query: 186 RIVAGKRYHGD--EVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSL 245
           R+++GKRY+GD  +VSD EEA++FRE++ E+ +  GA+++ D+LP LL+ +  D  E+  
Sbjct: 187 RMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLP-LLRVVDLDNLEKRC 246

Query: 246 VRLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFA 305
            R+ KR++ F++ LIEE R         +++D LL+L  ++P+YY+D +IKGLI  +L A
Sbjct: 247 KRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLA 306

Query: 306 GTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLR 365
           GTD+S+ T+EW M+ELL   +VL KAK ELDT+IG+++ +DE DL+ L YLQ I+SETLR
Sbjct: 307 GTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLR 366

Query: 366 LHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVAN 425
           LHP AP+LLPHYSS+DCT+  +++P+DT++L N W IHRDP+ W+D  SF+PERF     
Sbjct: 367 LHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF---EK 426

Query: 426 ELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHK 485
           E + NK++AFGLGRRACPG S+AQ  +G T+GLLIQC+EW++  +E++DM E  GIT   
Sbjct: 427 EEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPM 486

Query: 486 VEPLEAMCKARPVMNKV 497
             PL AMCKA P+ N V
Sbjct: 487 KIPLRAMCKALPIANDV 499

[Close]

BLAST of Sgr021308 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 523.9 bits (1348), Expect = 2.1e-147
Identity = 254/497 (51.11%), Postives = 362/497 (72.84%), Query Frame = 0


Query: 5   ILAFLLLLLAILFLQQLRTRRR---NLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYGP 64
           +L++ +  LA+ F+  +  R R   NLPP PPSLPIIG+LH LKRP HR F  L+ KYG 
Sbjct: 6   LLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGH 65

Query: 65  IFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHWR 124
           +FSL FG RL V+VSS    ++CFTKND+V A+RPR L GKY+ YNYTT+ +  +G+HWR
Sbjct: 66  VFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWR 125

Query: 125 NLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAVM 184
           NLRR+ A+++ S  R+NS   IR+DE QR++ +L   SS  F ++EL +   ++TFN +M
Sbjct: 126 NLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIM 185

Query: 185 RIVAGKRYHGD--EVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSL 244
           R+++GKRY+G+  + SD +EA +FR+++ E+    GA+++ D++P LL+++  +  E+ L
Sbjct: 186 RMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMP-LLRFLDFENLEKRL 245

Query: 245 VRLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFA 304
             +  +TD F++ LIEE R KK  ER N+++D LL LQ ++P+YYTDQIIKGL L +L A
Sbjct: 246 KDISGKTDAFLRGLIEEHRTKK--ERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLA 305

Query: 305 GTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLR 364
           GTDSS+ TLEW+M+ LL   +VL K K ELDT +GQDR +DE+DL  L YL+ +++ETLR
Sbjct: 306 GTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLR 365

Query: 365 LHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVAN 424
           L+  AP+LLPH +SD+C + GY +P+DT++L+NAWAIHRDP LW + T+F+PERF     
Sbjct: 366 LYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERF---EK 425

Query: 425 ELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHK 484
           + +  KLIAFG+GRRACPG  +A   + +TL LLIQC++WK +  ++ID+AE  G T  K
Sbjct: 426 KGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTK 485

Query: 485 VEPLEAMCKARPVMNKV 497
           + PL+AMCK+RPV+NKV
Sbjct: 486 LVPLKAMCKSRPVINKV 496

[Close]

BLAST of Sgr021308 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 9.6e-145
Identity = 249/496 (50.20%), Postives = 361/496 (72.78%), Query Frame = 0


Query: 6   LAFLLLLLAILFLQQLRTRRR---NLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYGPI 65
           L + L  L+I F+ +L  + R   NLPP P SLPIIG+LH LKRP +R F  L  KYG +
Sbjct: 7   LCYSLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNV 66

Query: 66  FSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHWRN 125
            SL FG RL V+VSSL   +ECFTKND+V A+RPR L GKY+ YNYTT+ +  +G+HWRN
Sbjct: 67  ISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRN 126

Query: 126 LRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAVMR 185
           LRR+ ++++ S  R+N+   IR+DE QR++ KL   SS +F +VEL   F ++TFN +MR
Sbjct: 127 LRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMR 186

Query: 186 IVAGKRYHGD--EVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 245
           +++GKRY+GD  ++S+ +EA +FR+++ E+    GA+++ D++P LLK++  +  E+ + 
Sbjct: 187 MISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMP-LLKFLDFENLEKRVK 246

Query: 246 RLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAG 305
           R+ ++ D F+  L++E+R+KK  ER N+++D LL +Q ++P+YYTD IIKGL L +L AG
Sbjct: 247 RIGEKNDVFLSGLLQEQRSKK--ERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAG 306

Query: 306 TDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRL 365
           TDSS+ TLEW M+ +L   +VL K + E+DT +GQDR +DE+DL  L YL+ ++ ETLRL
Sbjct: 307 TDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRL 366

Query: 366 HPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANE 425
           +  AP+LLPH ++D+C + GY +PRDT++L+NAWAIHRDP  W + T+F+PERF     E
Sbjct: 367 YTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERF-DKKGE 426

Query: 426 LQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHKV 485
           L+  K+IAFG+GRRACPG  +A   + +TL LL+QC++WK++ DE+IDM+E  G T  K+
Sbjct: 427 LE--KMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTKL 486

Query: 486 EPLEAMCKARPVMNKV 497
            PL+AMCK RPV+NKV
Sbjct: 487 LPLKAMCKTRPVVNKV 496

[Close]

BLAST of Sgr021308 vs. ExPASy TrEMBL
Match: K7NC01 (Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 2.7e-235
Identity = 410/504 (81.35%), Postives = 454/504 (90.08%), Query Frame = 0


Query: 1   MDFLILAFLLLLLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYG 60
           MDF   AFLLLLL +L L Q+RTRRRNLPP PPSLPIIGHLHLLKRP HRNFHK+AA+YG
Sbjct: 1   MDF-FSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYG 60

Query: 61  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 120
           PIFSLRFG RLAVIVSSLDIAEECFTKND++FA+RPRLL+ K+LGYN TT+AT+P+GDHW
Sbjct: 61  PIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHW 120

Query: 121 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 180
           RNLRRLAA+EIFSTARLNS+LSIRKDEIQR+LLKL +GSSGEFTKVELKTMFSEL FNA+
Sbjct: 121 RNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNAL 180

Query: 181 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 240
           MRIVAGKRY+GDEVSDEEEAREFR LM EIS HGGASH VD++PI LKWI G GFE+SLV
Sbjct: 181 MRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPI-LKWIGGGGFEKSLV 240

Query: 241 RLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAG 300
           RL KRTDKFMQALIEERRNKK +ERKNSLLDRLLELQA+EP+YYTDQIIKGL+LVLL AG
Sbjct: 241 RLTKRTDKFMQALIEERRNKKVLERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300

Query: 301 TDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRL 360
           TD+S+ TL WAM +LL   ++L KAKAELDT+IGQDRP+DE DL NL+YLQAIVSETLRL
Sbjct: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360

Query: 361 HPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANE 420
           HPAAPMLL HYSS DCTVAGY IPR T LLVNAWAIHRDP+LWDDPTSFRPERF+G ANE
Sbjct: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420

Query: 421 LQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHKV 480
           LQS KLIAFGLGRR+CPG +MA  F+GLTLGLLIQCY+WKK GDE++DM E GGIT HK 
Sbjct: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480

Query: 481 EPLEAMCKARPVMNKVLLNTLDEI 505
           +PLEAMCKARP M K+LLN LD+I
Sbjct: 481 KPLEAMCKARPAMYKLLLNALDKI 502

[Close]

BLAST of Sgr021308 vs. ExPASy TrEMBL
Match: A0A6J1FUQ9 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 5.9e-206
Identity = 362/504 (71.83%), Postives = 428/504 (84.92%), Query Frame = 0


Query: 1   MDFLILAFLLLLLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYG 60
           MDFL +  LLLLL  LFLQQ  TRRRNLPP PPS PIIGHLHLL+RP HRNFH +AAKYG
Sbjct: 1   MDFLFV-LLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYG 60

Query: 61  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 120
           PIF+LRFG RLA+IVSSL +AEECFTKND+VFA+RPRLL+GKYLGYN+TTI+T P GDHW
Sbjct: 61  PIFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHW 120

Query: 121 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 180
           RNLRRL ++EIFSTARLN+ LSIRKDEI+R+L+KL + S   F KVELK+MFSEL+FN V
Sbjct: 121 RNLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVV 180

Query: 181 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 240
           MR+V GKRY G+EVS+E EAREFRELM E S HGGAS+ VD++PI LKWI    +E+SL 
Sbjct: 181 MRVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPI-LKWIGFGEYEKSLA 240

Query: 241 RLRKRTDKFMQALIEERRNKKA--IERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLF 300
           +L KR DKFMQ L+E+RRN+    IE++++LLDRLLELQA+EP+YY+D+IIKG+ILVLL 
Sbjct: 241 KLTKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLR 300

Query: 301 AGTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETL 360
           AG DS+S T+EWAMT+LL   +V++KAKAE+DT+IG+DR IDEADL NL+YLQAI+SET 
Sbjct: 301 AGADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETF 360

Query: 361 RLHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVA 420
           RLHPAAPMLL HYSS+DCTVAGY +PR TMLLVNAWAIHRDP+LWDDP SFRPERF+G  
Sbjct: 361 RLHPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEG 420

Query: 421 NELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGH 480
           NEL  NKLI FG+GRRACPG +MA   +GL+LGLLIQCYEWK VG E++DM E GGIT  
Sbjct: 421 NELLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQ 480

Query: 481 KVEPLEAMCKARPVMNKVLLNTLD 503
           KV+PLE MC+A P+M+KVL N LD
Sbjct: 481 KVQPLETMCRALPIMDKVLSNGLD 499

[Close]

BLAST of Sgr021308 vs. ExPASy TrEMBL
Match: A0A6J1D806 (cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.9e-205
Identity = 372/509 (73.08%), Postives = 432/509 (84.87%), Query Frame = 0


Query: 1   MDFLILAFLLL--LLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAK 60
           MD L +  LLL  L +ILF QQ+R  RRNLPP PPS+PIIGHL LL+RP HRNF K+AA+
Sbjct: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60

Query: 61  YGPIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGD 120
           YGPIFSLRFG RLAV+VSSLD AEEC TKND+VFA+RPRL++GK+LGYN+TT+A AP+G 
Sbjct: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120

Query: 121 HWRNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLR----TGSSGEFTKVELKTMFSE 180
           HWRNLRRL A E+FSTARL S+LSIRK+EI+R+LLKLR    +GS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180

Query: 181 LTFNAVMRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGD- 240
           +TFN VMR+VAGKRY+G+EVSDE EAREF ELM EIS HGGAS+ VD++PI L+WI  D 
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPI-LRWIGCDG 240

Query: 241 GFEQSLVRLRKRTDKFMQALIEE-RRNKK--AIERKN--SLLDRLLELQAAEPQYYTDQI 300
           G+E++L +L KRTDKFM+AL+EE R NKK  AIERKN  SLLDRLLELQ +EPQYYTDQI
Sbjct: 241 GYERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQI 300

Query: 301 IKGLILVLLFAGTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLN 360
           +KGL+LVLL AGTDS+S T+ WAMT+LL   +VLTKAKAELDT++GQDR +DE+DL NL 
Sbjct: 301 VKGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLK 360

Query: 361 YLQAIVSETLRLHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTS 420
           YLQAIV ETLRLHP APML+ HYSS DCTVAGY IP DT+LLVNAWAIHRDP+LWDDPTS
Sbjct: 361 YLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTS 420

Query: 421 FRPERFIG-VANELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEI 480
           FRPERF+G  ANEL SNKLIAFGLGRRACPGA+M + F+GLTL L+IQCYEWKK G+E I
Sbjct: 421 FRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENI 480

Query: 481 DMAESGGITGHKVEPLEAMCKARPVMNKV 497
           DM E GGIT  K +PLE MCKARPVM+KV
Sbjct: 481 DMGEGGGITILKAKPLEVMCKARPVMDKV 508

[Close]

BLAST of Sgr021308 vs. ExPASy TrEMBL
Match: A0A6J1J5B5 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 5.0e-205
Identity = 356/504 (70.63%), Postives = 431/504 (85.52%), Query Frame = 0


Query: 1   MDFLILAFLLLLLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAKYG 60
           MDFL + FLLLLL  LFLQQ  TRRRNLPP PPS+PIIGHLHLL+RP H+NFH +AAKYG
Sbjct: 1   MDFLSV-FLLLLLVFLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAKYG 60

Query: 61  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 120
           PIF+LRFG RLA+IVSSL +AEECFTKND+VFA+RPRLL+GKYLGYN+TTI+T P+GD W
Sbjct: 61  PIFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDLW 120

Query: 121 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 180
           RNLRRL ++E+FSTARLN+ LSIRKDEI+R+L+KL + S   F KVELK+MFSEL+FN V
Sbjct: 121 RNLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVV 180

Query: 181 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 240
           MR+V GKRY G+EVS+E EAREFRELM EIS HGGAS+ ++++PI LKWI    +E+SL 
Sbjct: 181 MRVVTGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPI-LKWIGFGEYEKSLA 240

Query: 241 RLRKRTDKFMQALIEERRNKKA--IERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLF 300
           +L KR DKFMQ L+E RRN+    IE++++LLDRLLELQA+EP+YY+D+IIKG++LVLL 
Sbjct: 241 KLTKRADKFMQKLVEYRRNQTGFEIEKQSTLLDRLLELQASEPEYYSDEIIKGVVLVLLR 300

Query: 301 AGTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETL 360
           AG DS+S T+EWAMT+LL   +V++KAKAE+DT+IG+DR +DE+DL NL+YLQAI+ ET 
Sbjct: 301 AGVDSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETF 360

Query: 361 RLHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVA 420
           RLHPAAPMLL HYSSDDCTVAGY IPR TML+VNAWAIHRDP+LWDDP SFRPERF+G  
Sbjct: 361 RLHPAAPMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAG 420

Query: 421 NELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGH 480
           NEL SNKL+ FG+GRRACPG +MAQ  +GL+LGLLIQCYEWK VG +++DM E GGIT  
Sbjct: 421 NELLSNKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQ 480

Query: 481 KVEPLEAMCKARPVMNKVLLNTLD 503
           KV+PLE MC+A P+M+KVL N LD
Sbjct: 481 KVQPLETMCRALPIMDKVLSNGLD 499

[Close]

BLAST of Sgr021308 vs. ExPASy TrEMBL
Match: A0A6J1D8F1 (cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 1.2e-203
Identity = 372/512 (72.66%), Postives = 432/512 (84.38%), Query Frame = 0


Query: 1   MDFLILAFLLL--LLAILFLQQLRTRRRNLPPCPPSLPIIGHLHLLKRPTHRNFHKLAAK 60
           MD L +  LLL  L +ILF QQ+R  RRNLPP PPS+PIIGHL LL+RP HRNF K+AA+
Sbjct: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60

Query: 61  YGPIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGD 120
           YGPIFSLRFG RLAV+VSSLD AEEC TKND+VFA+RPRL++GK+LGYN+TT+A AP+G 
Sbjct: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120

Query: 121 HWRNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLR----TGSSGEFTKVELKTMFSE 180
           HWRNLRRL A E+FSTARL S+LSIRK+EI+R+LLKLR    +GS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180

Query: 181 LTFNAVMRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGD- 240
           +TFN VMR+VAGKRY+G+EVSDE EAREF ELM EIS HGGAS+ VD++PI L+WI  D 
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPI-LRWIGCDG 240

Query: 241 GFEQSLVRLRKRTDKFMQALIEE-RRNKK--AIERKN--SLLDRLLELQAAEPQYYTDQI 300
           G+E++L +L KRTDKFM+AL+EE R NKK  AIERKN  SLLDRLLELQ +EPQYYTDQI
Sbjct: 241 GYERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQI 300

Query: 301 IKGLIL---VLLFAGTDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLT 360
           +KGL+L   VLL AGTDS+S T+ WAMT+LL   +VLTKAKAELDT++GQDR +DE+DL 
Sbjct: 301 VKGLVLNMQVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLA 360

Query: 361 NLNYLQAIVSETLRLHPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDD 420
           NL YLQAIV ETLRLHP APML+ HYSS DCTVAGY IP DT+LLVNAWAIHRDP+LWDD
Sbjct: 361 NLKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDD 420

Query: 421 PTSFRPERFIG-VANELQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGD 480
           PTSFRPERF+G  ANEL SNKLIAFGLGRRACPGA+M + F+GLTL L+IQCYEWKK G+
Sbjct: 421 PTSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGE 480

Query: 481 EEIDMAESGGITGHKVEPLEAMCKARPVMNKV 497
           E IDM E GGIT  K +PLE MCKARPVM+KV
Sbjct: 481 ENIDMGEGGGITILKAKPLEVMCKARPVMDKV 511

[Close]

BLAST of Sgr021308 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 535.8 bits (1379), Expect = 3.7e-152
Identity = 271/496 (54.64%), Postives = 366/496 (73.79%), Query Frame = 0


Query: 4   LILAFLLLLLAILFLQQLRTRRRNLPPCPP-SLPIIGHLHLLKRPTHRNFHKLAAKY--G 63
           LI + L ++L++++L     R+ NLPP P  SLP+IGHL LLK P HR F  L+      
Sbjct: 6   LIFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNA 65

Query: 64  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 123
           PIFSLR G RL  + SS  IAEECFTKND+V A+RP  ++ K++ Y+YTT+  A +GDHW
Sbjct: 66  PIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHW 125

Query: 124 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 183
           RNLRR+ +VEIFS  RLNS LSIRKDEI+R++ +L    S EF KV++K+M S+LTFN +
Sbjct: 126 RNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNI 185

Query: 184 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 243
           +R+VAGKRY+GD V D+ EA+  R+L+ ++ A  GA + VDYLP+L   +V D +E  + 
Sbjct: 186 LRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLR--LVSD-YETRVK 245

Query: 244 RLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAG 303
           +L  R D+F+Q L++E+R  K  E+ N+++D LL LQ ++P Y+TD+IIKG +L L+ AG
Sbjct: 246 KLAGRLDEFLQGLVDEKREAK--EKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAG 305

Query: 304 TDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRL 363
           TD+S+ TLEWA++ +L    VL KA+ E+D +IG DR +DE+D++NL YLQ IVSETLRL
Sbjct: 306 TDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRL 365

Query: 364 HPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANE 423
           +PAAPMLLPH +S+DC VAGY +PR T+LL N WAIHRDP+LWDDP SF+PERF     E
Sbjct: 366 YPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERF---EKE 425

Query: 424 LQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHKV 483
            ++ KL+ FGLGRRACPG+ +A   + LTLG LIQC EW+K+G EE+DM+E  G+T  K 
Sbjct: 426 GEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIG-EEVDMSEGKGVTMPKA 485

Query: 484 EPLEAMCKARPVMNKV 497
           +PLEAMC+ARP + K+
Sbjct: 486 KPLEAMCRARPSVVKI 492

[Close]

BLAST of Sgr021308 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 527.3 bits (1357), Expect = 1.3e-149
Identity = 268/498 (53.82%), Postives = 360/498 (72.29%), Query Frame = 0


Query: 4   LILAFLLLLLAILFLQQLRTRRRNLPPCPP-SLPIIGHLHLLKRPTHRNFHKLAAKYG-- 63
           LI  FL + L++ F+     RR NLPP P  +LP+IGHL LLK P HR F  ++   G  
Sbjct: 6   LIFTFLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDA 65

Query: 64  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 123
           PI SLR G RL  +VSS  +AEECFTKND+V A+R   L  K++ Y  TT+ TA +GDHW
Sbjct: 66  PIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHW 125

Query: 124 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 183
           RNLRR+ AVEIFS  RLNS  SIR+DEI R++  L   SS EFTKVE+K+MFS LTFN +
Sbjct: 126 RNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNI 185

Query: 184 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 243
           +R++AGK Y+GD   D+ EA+  REL+ E     GA +  DYLPI L WI G   E+ + 
Sbjct: 186 IRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPI-LTWITGS--EKRIK 245

Query: 244 RLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAG 303
           ++  R D+F+Q L++ERR  K  +R+N+++D LL LQ  +P+YYTD IIKG++L L+ AG
Sbjct: 246 KIASRLDEFLQGLVDERREGKE-KRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAG 305

Query: 304 TDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRL 363
           TD+S+ TLEW ++ LL   ++L+KA+ E+D ++G +R ++E+DL++L YLQ IVSE+LRL
Sbjct: 306 TDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRL 365

Query: 364 HPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANE 423
           +PA+P+L+PH +S+DC V GY +PR TMLL NAWAIHRDP++WDDPTSF+PERF     E
Sbjct: 366 YPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERF---EKE 425

Query: 424 LQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAE-SGGITGHK 483
            ++ KL+ FGLGRRACPG+ +AQ    LT+G LIQC+EW+++G+EE+DM E  GG+   K
Sbjct: 426 GEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPK 485

Query: 484 VEPLEAMCKARPVMNKVL 498
             PL AMCKARPV+ K+L
Sbjct: 486 AIPLVAMCKARPVVGKIL 496

[Close]

BLAST of Sgr021308 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 518.5 bits (1334), Expect = 6.2e-147
Identity = 259/497 (52.11%), Postives = 351/497 (70.62%), Query Frame = 0


Query: 4   LILAFLLLLLAILFLQQLRTRRRNLPPCPP-SLPIIGHLHLLKRPTHRNFHKLAAKYG-- 63
           L+L+FL L ++I  L     R+ NLPP P   LP+IGHLHLLK+P HR FH ++   G  
Sbjct: 7   LLLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNA 66

Query: 64  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 123
           PIF LR G RL  ++SS  IAEECFTKND+V A+RP +++ K++GYN+T +  A +GDHW
Sbjct: 67  PIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHW 126

Query: 124 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 183
           RNLRR+AAVEIFS+ R+++  SIRKDEI+R++  L   S   F +VELK++ + L FN +
Sbjct: 127 RNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNI 186

Query: 184 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 243
           + +VAGKRY+G    D +EA+  REL+ EI A  G+ +  DYLP  + W+    FE    
Sbjct: 187 IMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLP-SINWVT--NFENQTK 246

Query: 244 RLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAG 303
            L  R D+ +Q L++E+R +K  E+  +L+D LL  Q  EP+YYTD IIKG+IL L+ AG
Sbjct: 247 ILGNRLDRVLQKLVDEKRAEK--EKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAG 306

Query: 304 TDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRL 363
           TD+SS TLEWAM+ LL   ++L KA+AE+D +IG DR ++E+D+ NL+YLQ IVSETLRL
Sbjct: 307 TDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRL 366

Query: 364 HPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANE 423
           +PA P+LLPH+SSD+C VAGY +PR T+LL N WA+HRDP LW++P  F+PERF     E
Sbjct: 367 YPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERF---EKE 426

Query: 424 LQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHKV 483
            ++ KL+ FG+GRRACPGA + +  + L LG LIQ +EW++VG E +DM E  GIT  K 
Sbjct: 427 GEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPKA 486

Query: 484 EPLEAMCKARPVMNKVL 498
            PL AMCKAR ++ K +
Sbjct: 487 TPLRAMCKARAIVGKTI 495

[Close]

BLAST of Sgr021308 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 508.1 bits (1307), Expect = 8.3e-144
Identity = 259/497 (52.11%), Postives = 353/497 (71.03%), Query Frame = 0


Query: 4   LILAFLLLLLAILFLQQLRTRRRNLPPCPP-SLPIIGHLHLLKRPTHRNFHKLAAKYG-- 63
           LI  F  ++L+++FL     R+ NLPP P  +LP+IGHL LLK P HR F  ++   G  
Sbjct: 6   LIFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDA 65

Query: 64  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 123
           PI SLR G RL  +VSS  IAEECFTKND++ A+R   +  K++ Y  +T+ +A + +HW
Sbjct: 66  PIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHW 125

Query: 124 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 183
           RNLRR+ A+EIFS  RLNS  SIR+DEI+R++ +L   SS  FTKVE+K+MFS+LTFN +
Sbjct: 126 RNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNI 185

Query: 184 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 243
           +R++AGK Y+GD   D+ EA+  R L+ E  +  G  +  DY+PI L WI     E  + 
Sbjct: 186 IRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPI-LTWITYS--ETRIK 245

Query: 244 RLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAG 303
           +L  R D+F+Q L++E+R  K  +++N+++D LL LQ  +P+YY D+IIKG +L L+  G
Sbjct: 246 KLAGRLDEFLQGLVDEKREGKE-KKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGG 305

Query: 304 TDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRL 363
           TD+++ TLEWA++ LL   +VL KA+ E+D  IG DR ++E+D+ NL YLQ IVSETLRL
Sbjct: 306 TDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRL 365

Query: 364 HPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANE 423
           +PAAPMLLPH +S DC V GY +PR TMLL NAWAIHRDP LWDDPTSF+PERF     E
Sbjct: 366 YPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERF---EKE 425

Query: 424 LQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHKV 483
            ++ KL+ FGLGRRACPG+ +AQ  + L+LG LIQC+EW+++G+EE+DM E  G+T  K 
Sbjct: 426 GEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKA 485

Query: 484 EPLEAMCKARPVMNKVL 498
            PLEAMC+AR  + K+L
Sbjct: 486 RPLEAMCRARDFVGKIL 495

[Close]

BLAST of Sgr021308 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 506.1 bits (1302), Expect = 3.2e-143
Identity = 256/497 (51.51%), Postives = 348/497 (70.02%), Query Frame = 0


Query: 4   LILAFLLLLLAILFLQQLRTRRRNLPPCPP-SLPIIGHLHLLKRPTHRNFHKLAAKYG-- 63
           LIL+FL L    +FL     R+ NLPP P  SLP+IGHLHLLK P HR F  L+   G  
Sbjct: 7   LILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNA 66

Query: 64  PIFSLRFGPRLAVIVSSLDIAEECFTKNDIVFADRPRLLVGKYLGYNYTTIATAPHGDHW 123
           P+F LR G RL  ++SS  IAEECFTKND+V A+RP+  + K+LGYN T + +A +GDHW
Sbjct: 67  PVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHW 126

Query: 124 RNLRRLAAVEIFSTARLNSTLSIRKDEIQRMLLKLRTGSSGEFTKVELKTMFSELTFNAV 183
           RNLRR+AAVEIFST RLNS L IRKDEI+R++  L   S   F +VE+KT+ + L  N  
Sbjct: 127 RNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTT 186

Query: 184 MRIVAGKRYHGDEVSDEEEAREFRELMVEISAHGGASHRVDYLPILLKWIVGDGFEQSLV 243
           +R++AGKRY G+   D ++A+  + L+ E     GA + +DYL I L+W+    +E+ + 
Sbjct: 187 IRMLAGKRYFGE---DNDDAKLVKNLVSEAVTSAGAGNPIDYLSI-LRWV--SSYEKRIK 246

Query: 244 RLRKRTDKFMQALIEERRNKKAIERKNSLLDRLLELQAAEPQYYTDQIIKGLILVLLFAG 303
            L  R D F+Q L++E+R +K  E+  +++D LL LQ  +P YYTD IIKG+IL L+ AG
Sbjct: 247 NLGNRFDTFLQKLVDEKRAEK--EKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAG 306

Query: 304 TDSSSATLEWAMTELLRKTKVLTKAKAELDTEIGQDRPIDEADLTNLNYLQAIVSETLRL 363
           TD+SS TLEWAM+ LL   ++L KA+ E+D ++G DR +DE+D+ NL+YLQ+IV ETLR+
Sbjct: 307 TDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRM 366

Query: 364 HPAAPMLLPHYSSDDCTVAGYSIPRDTMLLVNAWAIHRDPRLWDDPTSFRPERFIGVANE 423
           +PA P+LLPH SS+DC V GY IP  TM+L NAWA+HRDP +W+DP  F+PERF     E
Sbjct: 367 YPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERF---EKE 426

Query: 424 LQSNKLIAFGLGRRACPGASMAQHFLGLTLGLLIQCYEWKKVGDEEIDMAESGGITGHKV 483
            ++ KLI+FG+GRRACPGA +A   +   LG L+QC+EW++VG++ +DM E  G T  K 
Sbjct: 427 GEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKA 486

Query: 484 EPLEAMCKARPVMNKVL 498
            PL AMCKAR +++K++
Sbjct: 487 IPLRAMCKARSIVDKLI 492

The following BLAST results are available for this feature:

BLAST of Sgr021308 vs. NCBI nr


Match Name	E-value	Identity	Description
AEM42992.1	5.6e-235	81.35	cytochrome P450 [Siraitia grosvenorii]	[more]
XP_022944057.1	1.2e-205	71.83	cytochrome P450 81E8-like [Cucurbita moschata] >KAG7011030.1 Cytochrome P450 81E...	[more]
XP_023512643.1	1.6e-205	71.23	cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo]	[more]
XP_022149457.1	6.1e-205	73.08	cytochrome P450 81E8-like isoform X2 [Momordica charantia]	[more]
XP_022985607.1	1.0e-204	70.63	cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E...	[more]

BLAST of Sgr021308 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6WNQ8	1.1e-156	56.44	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
W8JMU7	1.6e-152	54.31	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ9	5.4e-148	52.31	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
P93147	2.1e-147	51.11	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNR0	9.6e-145	50.20	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of Sgr021308 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
K7NC01	2.7e-235	81.35	Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1	[more]
A0A6J1FUQ9	5.9e-206	71.83	cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=...	[more]
A0A6J1D806	2.9e-205	73.08	cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1J5B5	5.0e-205	70.63	cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1	[more]
A0A6J1D8F1	1.2e-203	72.66	cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]

BLAST of Sgr021308 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37370.1	3.7e-152	54.64	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37340.1	1.3e-149	53.82	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT4G37320.1	6.2e-147	52.11	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT4G37360.1	8.3e-144	52.11	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT4G37330.1	3.2e-143	51.51	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 259..279
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 8..459 e-value: 2.0E-17 score: 61.1
None	No IPR available	PIRSR	PIRSR000209-1	PIRSR000209-1	coord: 30..472 e-value: 3.4E-39 score: 132.1
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 5..496
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 5..496
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 352..363 score: 42.16 coord: 427..436 score: 51.99 coord: 299..316 score: 36.05
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 29..470 e-value: 1.6E-94 score: 317.3
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 288..305 score: 29.36 coord: 436..459 score: 30.89 coord: 175..193 score: 22.03 coord: 80..101 score: 24.17 coord: 308..334 score: 32.05 coord: 392..416 score: 38.07 coord: 426..436 score: 49.59 coord: 351..369 score: 37.86 coord: 56..75 score: 37.19
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 15..490 e-value: 5.2E-125 score: 419.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 29..490
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 429..438

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr021308.1	Sgr021308.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen