Sgr021215 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr021215
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Non-specific serine/threonine protein kinase
Location	tig00153653: 182518 .. 184239 (-)
RNA-Seq Expression	Sgr021215
Synteny	Sgr021215

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCCCCAGTTGTTGGGATGTTTATCTATGGTTGGACGTAAATTTGGATGGTTAGAGTGGATTGAGAATTTGTGGAAGTGCTTGACTCTTTTGGCGGAAATATTACGTGAACATTTTTCCACCTTTTATCCACTTGCAATTGATATCCTATTCCAAAGTTTGGAAATGACTAAGGCTAACCGTGTAGTGAGACGTCAGAAGATTACGTTTCTTCAAGTTCACGGTGTTTTAAAAACTAATCTTCAGTTATTGTCCCTTCAAAAACTTGGATTGCTGCCATCATCTGTGCACAGAATATTGCAATTTGATGCACCAATATCTCAGCTTCGAATGCATCCAAATCACTTGGTGACGGGAAGCTCTGCTGCCACTTATATATTCTTGCTCCAACATGGGAATAATGAAGTTGTTGAACAAACAGTGACGTTATTAATTGAAGAGTTAGAAATGTTCAAGGGCTTGTTAGAAAAAGGTATAGATCAAGGAGATGTCAATGGTGTCCTTGATTCTCAATTCTATTCGAATATTGATTTGTCTGCCCTCATTAAATTTGATTTGAGAGTCTTATTGACATGTACTATCTCTAGTGGAACTGTTGGTTTGATAGGCCAAGAAAAGGTTGCTTTCGCATGTCTAAAAAGGTCAGAAAGATTGATTTCCTTTATAATGAAGAAATTGAATCCTTTTGACTTTCCAGTTCAGGCTTATGTGGAATTGCAGGTTGGTATTCTCAATACATTGGACAGGTTGACTGCAACTGAATTCTTTAGCAAGTTCTCTTTGAAGAAATTGAGTAGCGAGAATCATTTTGTGGATATAGGTGAAAAGATAGATGAAGACTTTCTCAAGAAGGAGCCTTCGGCTATTATTATTGAGCAACTAACGAAATACAATGTGCTCTTCTCCAAAGCCCTTAATAAATCTTCTCCACTAACAGTTAAAGTAACAACCCTGGGTTGGATTCAAAGATTCTGTGAGAATGTTATTACTATTTTTAAGAATGACAAAACGTATGCTAATTTTTTTGAAGCATTTGGATATGTTGGTGGCGTAGGAAACTTGATTTTTATGGTAATTGATGCTGCATCTGACAGGGAACCTAAGGTGAGATCTAATGCAGCTTCAGTATTGGAGCTGCTTCTGCTTGCAAGAATTGTGCATCCCATGTACTTTTATCCCATTGCTGATGTAGTCCTGGAGAAACTTGGTGATCCAGATGATGATATAAAAAACTCATTTGTGAGATTGCTTTCTCATGTCTTGCCCACGGCACTTTATGCATGTGGTCTGTATGACGTTGGAGTATATCCTGCTTGTAGGCCCCATCTTTTGATGTCTGATCATAAATCTAGCCTGCATTGGAAACAAGTATTTGCCTTGAAGCAGCTTCCTCAGCAAATTCATTTTCAACAACTTATTTCCATCTTGAGCTACATATCACAAAGATGGAAAGTTCCTGTGGCTTCATGGATGCAGCGGCTCATCCATAGATGTGGGAGATTGAAGGACATTGATTTGAGTCAGAGTGAGGAAACAGGAAACTTAGGTGCAAATGGTTTATGGTTGGACTTTAAGGTGGATGATGAGTTTTTCTATGGGAATTGCTCAGTTAATTGTGTAGCTGGACTCTGGTGGGCCATTCATGAAGCAGCTAGATATTGTATTGCTCTGCGTCTACGAACGAACCTTGGTGGGCCTACACAACTTTTGCAGCACTAG

mRNA sequence

ATGCTTCCCCAGTTGTTGGGATGTTTATCTATGGTTGGACGTAAATTTGGATGGTTAGAGTGGATTGAGAATTTGTGGAAGTGCTTGACTCTTTTGGCGGAAATATTACGTGAACATTTTTCCACCTTTTATCCACTTGCAATTGATATCCTATTCCAAAGTTTGGAAATGACTAAGGCTAACCGTGTAGTGAGACGTCAGAAGATTACGTTTCTTCAAGTTCACGGTGTTTTAAAAACTAATCTTCAGTTATTGTCCCTTCAAAAACTTGGATTGCTGCCATCATCTGTGCACAGAATATTGCAATTTGATGCACCAATATCTCAGCTTCGAATGCATCCAAATCACTTGGTGACGGGAAGCTCTGCTGCCACTTATATATTCTTGCTCCAACATGGGAATAATGAAGTTGTTGAACAAACAGTGACGTTATTAATTGAAGAGTTAGAAATGTTCAAGGGCTTGTTAGAAAAAGGTATAGATCAAGGAGATGTCAATGGTGTCCTTGATTCTCAATTCTATTCGAATATTGATTTGTCTGCCCTCATTAAATTTGATTTGAGAGTCTTATTGACATGTACTATCTCTAGTGGAACTGTTGGTTTGATAGGCCAAGAAAAGGTTGCTTTCGCATGTCTAAAAAGGTCAGAAAGATTGATTTCCTTTATAATGAAGAAATTGAATCCTTTTGACTTTCCAGTTCAGGCTTATGTGGAATTGCAGGTTGGTATTCTCAATACATTGGACAGGTTGACTGCAACTGAATTCTTTAGCAAGTTCTCTTTGAAGAAATTGAGTAGCGAGAATCATTTTGTGGATATAGGTGAAAAGATAGATGAAGACTTTCTCAAGAAGGAGCCTTCGGCTATTATTATTGAGCAACTAACGAAATACAATGTGCTCTTCTCCAAAGCCCTTAATAAATCTTCTCCACTAACAGTTAAAGTAACAACCCTGGGTTGGATTCAAAGATTCTGTGAGAATGTTATTACTATTTTTAAGAATGACAAAACGTATGCTAATTTTTTTGAAGCATTTGGATATGTTGGTGGCGTAGGAAACTTGATTTTTATGGTAATTGATGCTGCATCTGACAGGGAACCTAAGGTGAGATCTAATGCAGCTTCAGTATTGGAGCTGCTTCTGCTTGCAAGAATTGTGCATCCCATGTACTTTTATCCCATTGCTGATGTAGTCCTGGAGAAACTTGGTGATCCAGATGATGATATAAAAAACTCATTTGTGAGATTGCTTTCTCATGTCTTGCCCACGGCACTTTATGCATGTGGTCTGTATGACGTTGGAGTATATCCTGCTTGTAGGCCCCATCTTTTGATGTCTGATCATAAATCTAGCCTGCATTGGAAACAAGTATTTGCCTTGAAGCAGCTTCCTCAGCAAATTCATTTTCAACAACTTATTTCCATCTTGAGCTACATATCACAAAGATGGAAAGTTCCTGTGGCTTCATGGATGCAGCGGCTCATCCATAGATGTGGGAGATTGAAGGACATTGATTTGAGTCAGAGTGAGGAAACAGGAAACTTAGGTGCAAATGGTTTATGGTTGGACTTTAAGGTGGATGATGAGTTTTTCTATGGGAATTGCTCAGTTAATTGTGTAGCTGGACTCTGGTGGGCCATTCATGAAGCAGCTAGATATTGTATTGCTCTGCGTCTACGAACGAACCTTGGTGGGCCTACACAACTTTTGCAGCACTAG

Coding sequence (CDS)

ATGCTTCCCCAGTTGTTGGGATGTTTATCTATGGTTGGACGTAAATTTGGATGGTTAGAGTGGATTGAGAATTTGTGGAAGTGCTTGACTCTTTTGGCGGAAATATTACGTGAACATTTTTCCACCTTTTATCCACTTGCAATTGATATCCTATTCCAAAGTTTGGAAATGACTAAGGCTAACCGTGTAGTGAGACGTCAGAAGATTACGTTTCTTCAAGTTCACGGTGTTTTAAAAACTAATCTTCAGTTATTGTCCCTTCAAAAACTTGGATTGCTGCCATCATCTGTGCACAGAATATTGCAATTTGATGCACCAATATCTCAGCTTCGAATGCATCCAAATCACTTGGTGACGGGAAGCTCTGCTGCCACTTATATATTCTTGCTCCAACATGGGAATAATGAAGTTGTTGAACAAACAGTGACGTTATTAATTGAAGAGTTAGAAATGTTCAAGGGCTTGTTAGAAAAAGGTATAGATCAAGGAGATGTCAATGGTGTCCTTGATTCTCAATTCTATTCGAATATTGATTTGTCTGCCCTCATTAAATTTGATTTGAGAGTCTTATTGACATGTACTATCTCTAGTGGAACTGTTGGTTTGATAGGCCAAGAAAAGGTTGCTTTCGCATGTCTAAAAAGGTCAGAAAGATTGATTTCCTTTATAATGAAGAAATTGAATCCTTTTGACTTTCCAGTTCAGGCTTATGTGGAATTGCAGGTTGGTATTCTCAATACATTGGACAGGTTGACTGCAACTGAATTCTTTAGCAAGTTCTCTTTGAAGAAATTGAGTAGCGAGAATCATTTTGTGGATATAGGTGAAAAGATAGATGAAGACTTTCTCAAGAAGGAGCCTTCGGCTATTATTATTGAGCAACTAACGAAATACAATGTGCTCTTCTCCAAAGCCCTTAATAAATCTTCTCCACTAACAGTTAAAGTAACAACCCTGGGTTGGATTCAAAGATTCTGTGAGAATGTTATTACTATTTTTAAGAATGACAAAACGTATGCTAATTTTTTTGAAGCATTTGGATATGTTGGTGGCGTAGGAAACTTGATTTTTATGGTAATTGATGCTGCATCTGACAGGGAACCTAAGGTGAGATCTAATGCAGCTTCAGTATTGGAGCTGCTTCTGCTTGCAAGAATTGTGCATCCCATGTACTTTTATCCCATTGCTGATGTAGTCCTGGAGAAACTTGGTGATCCAGATGATGATATAAAAAACTCATTTGTGAGATTGCTTTCTCATGTCTTGCCCACGGCACTTTATGCATGTGGTCTGTATGACGTTGGAGTATATCCTGCTTGTAGGCCCCATCTTTTGATGTCTGATCATAAATCTAGCCTGCATTGGAAACAAGTATTTGCCTTGAAGCAGCTTCCTCAGCAAATTCATTTTCAACAACTTATTTCCATCTTGAGCTACATATCACAAAGATGGAAAGTTCCTGTGGCTTCATGGATGCAGCGGCTCATCCATAGATGTGGGAGATTGAAGGACATTGATTTGAGTCAGAGTGAGGAAACAGGAAACTTAGGTGCAAATGGTTTATGGTTGGACTTTAAGGTGGATGATGAGTTTTTCTATGGGAATTGCTCAGTTAATTGTGTAGCTGGACTCTGGTGGGCCATTCATGAAGCAGCTAGATATTGTATTGCTCTGCGTCTACGAACGAACCTTGGTGGGCCTACACAACTTTTGCAGCACTAG

Protein sequence

MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKANRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTGSSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLSALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVELQVGILNTLDRLTATEFFSKFSLKKLSSENHFVDIGEKIDEDFLKKEPSAIIIEQLTKYNVLFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVGGVGNLIFMVIDAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDIKNSFVRLLSHVLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYISQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDEFFYGNCSVNCVAGLWWAIHEAARYCIALRLRTNLGGPTQLLQH

Homology

[Close]

BLAST of Sgr021215 vs. NCBI nr
Match: XP_022148544.1 (serine/threonine-protein kinase SMG1-like [Momordica charantia])

HSP 1 Score: 1025.4 bits (2650), Expect = 1.9e-295
Identity = 506/569 (88.93%), Postives = 540/569 (94.90%), Query Frame = 0


Query: 1   MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKA 60
           MLPQLLGCLSMVG KFGWLEWIENLWKCLTLLAEILRE FSTFYPLAIDIL QSLEMT+A
Sbjct: 315 MLPQLLGCLSMVGHKFGWLEWIENLWKCLTLLAEILRERFSTFYPLAIDILSQSLEMTRA 374

Query: 61  NRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTG 120
           N V+R QKITFLQVHGVLKTNLQLLSLQKLGLL SSVHRILQFDAPISQLR+HPNHLVTG
Sbjct: 375 NHVMRGQKITFLQVHGVLKTNLQLLSLQKLGLLSSSVHRILQFDAPISQLRLHPNHLVTG 434

Query: 121 SSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLS 180
           S+AATYIFLLQH NNEVVE+T TLL+EELEMFKGLLEKG+DQGDVN ++DSQ YSNIDL 
Sbjct: 435 SAAATYIFLLQHRNNEVVEKTATLLMEELEMFKGLLEKGLDQGDVNVIIDSQIYSNIDLF 494

Query: 181 ALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVEL 240
           AL+KFDL+VLLTCTISSGT+GLIGQE VAF+CL RSERLISFIM KLNPFDFP+QAYVEL
Sbjct: 495 ALVKFDLKVLLTCTISSGTIGLIGQENVAFSCLTRSERLISFIMNKLNPFDFPIQAYVEL 554

Query: 241 QVGILNTLDRLTATEFFSKFSLKKLSSENHFVDIGEKIDEDFLKKEPSAIIIEQLTKYNV 300
           QVGIL+TLDRLT TE FSK+SLKKLSSENHFVDIG+K+DEDFLKK+ SAIIIEQLTKYN 
Sbjct: 555 QVGILDTLDRLTTTELFSKWSLKKLSSENHFVDIGKKVDEDFLKKDHSAIIIEQLTKYNT 614

Query: 301 LFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVGGVGNLIFMVI 360
           LFSKAL+KSSPLTVK+TTL WIQRFCENVI IF NDKTYANF+EAFGYVG VGNLIFMVI
Sbjct: 615 LFSKALHKSSPLTVKITTLAWIQRFCENVIIIFNNDKTYANFYEAFGYVGVVGNLIFMVI 674

Query: 361 DAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDIKNSFVRLLSH 420
           DAASDREPKVRSNAASVLELL+LA+IVHP++FYP+AD+VLEKLGDPD+DIKNSFVRL+SH
Sbjct: 675 DAASDREPKVRSNAASVLELLMLAKIVHPIHFYPVADIVLEKLGDPDNDIKNSFVRLVSH 734

Query: 421 VLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 480
           VLPTALYACGLY+VG YP  RP LLMSDH+SSLHWKQVFALKQLPQQI+FQQLISILSYI
Sbjct: 735 VLPTALYACGLYNVGAYPTSRP-LLMSDHRSSLHWKQVFALKQLPQQIYFQQLISILSYI 794

Query: 481 SQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDEFFYGNCSVNC 540
           SQRWKVPVASW QRLIHRCGR KDIDLSQSEETGNLGANGLWLDFKVDDEF YGNCSVNC
Sbjct: 795 SQRWKVPVASWTQRLIHRCGRWKDIDLSQSEETGNLGANGLWLDFKVDDEFLYGNCSVNC 854

Query: 541 VAGLWWAIHEAARYCIALRLRTNLGGPTQ 570
           VAG+WWAIHEAARYCIALRLRTNLGGPTQ
Sbjct: 855 VAGIWWAIHEAARYCIALRLRTNLGGPTQ 882

[Close]

BLAST of Sgr021215 vs. NCBI nr
Match: XP_022986435.1 (serine/threonine-protein kinase SMG1-like [Cucurbita maxima])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.8e-293
Identity = 501/569 (88.05%), Postives = 532/569 (93.50%), Query Frame = 0


Query: 1   MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKA 60
           MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRE FSTFYPLAIDILFQ+LEMT+A
Sbjct: 315 MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRERFSTFYPLAIDILFQNLEMTRA 374

Query: 61  NRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTG 120
           N VVR  KITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLR+HPNHLVTG
Sbjct: 375 NHVVRGHKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRLHPNHLVTG 434

Query: 121 SSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLS 180
           SSAATYIFLLQHGNNEVVEQTVTLL EELE+FKGLLEKG+DQG++NG+L+SQFYS +DL 
Sbjct: 435 SSAATYIFLLQHGNNEVVEQTVTLLTEELEVFKGLLEKGLDQGNINGILESQFYSKMDLF 494

Query: 181 ALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVEL 240
           ALIKFDLR LLTCTISSGT+GLIGQE VA  CL+RSERLISFIMKKLNPFDFP+QAYVEL
Sbjct: 495 ALIKFDLRALLTCTISSGTIGLIGQENVALTCLRRSERLISFIMKKLNPFDFPIQAYVEL 554

Query: 241 QVGILNTLDRLTATEFFSKFSLKKLSSENHFVDIGEKIDEDFLKKEPSAIIIEQLTKYNV 300
           Q  ILNTLD LT TE FSK SL+KLSSE+HF+D GE+IDE FL K+ SAIIIEQLTKYN+
Sbjct: 555 QAAILNTLDSLTTTELFSKCSLRKLSSESHFLDAGEEIDETFLNKDHSAIIIEQLTKYNM 614

Query: 301 LFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVGGVGNLIFMVI 360
           LFSKAL+K+SPLTVK+TTLGWIQRFCENV+TIFKND TYANFFEAFGY G +GNLIFMVI
Sbjct: 615 LFSKALHKASPLTVKITTLGWIQRFCENVVTIFKNDTTYANFFEAFGYFGVIGNLIFMVI 674

Query: 361 DAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDIKNSFVRLLSH 420
           DAASDREPKVRSNAASVLELLL A+IVHP+YFYPIAD+VLEKLGDPD+DIKNSFVRLLSH
Sbjct: 675 DAASDREPKVRSNAASVLELLLQAKIVHPIYFYPIADIVLEKLGDPDNDIKNSFVRLLSH 734

Query: 421 VLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 480
           +LPTALYACG YD+G YPA R HLL SDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI
Sbjct: 735 ILPTALYACGQYDLGSYPASRLHLLRSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 794

Query: 481 SQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDEFFYGNCSVNC 540
           SQRWKVPVASW QRLIHRCGRLKDID SQSEETGN GANGLWLD KVDDEF  GNCSVNC
Sbjct: 795 SQRWKVPVASWTQRLIHRCGRLKDIDSSQSEETGNFGANGLWLDLKVDDEFLNGNCSVNC 854

Query: 541 VAGLWWAIHEAARYCIALRLRTNLGGPTQ 570
           VAG+WWAIHEAARYCI LRLRTNLGGPTQ
Sbjct: 855 VAGVWWAIHEAARYCITLRLRTNLGGPTQ 883

[Close]

BLAST of Sgr021215 vs. NCBI nr
Match: XP_023513297.1 (serine/threonine-protein kinase SMG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.5e-292
Identity = 499/569 (87.70%), Postives = 530/569 (93.15%), Query Frame = 0


Query: 1   MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKA 60
           MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRE FSTFYPLAIDILFQ+LEMT+A
Sbjct: 315 MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRERFSTFYPLAIDILFQNLEMTRA 374

Query: 61  NRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTG 120
           N VVR  KITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLR+HPNHLVTG
Sbjct: 375 NHVVRGHKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRLHPNHLVTG 434

Query: 121 SSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLS 180
           SSAATYIFLLQHGNNEVVEQTVTLL EELE+FKGLLEK +DQG++NG+L+SQFYS +DL 
Sbjct: 435 SSAATYIFLLQHGNNEVVEQTVTLLTEELEVFKGLLEKCLDQGNINGILESQFYSKMDLF 494

Query: 181 ALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVEL 240
           ALIKFDLR LLTCTISSGT+GLIGQE VA  CL+RSERLISFIMKKLNPFDFP+QAYVEL
Sbjct: 495 ALIKFDLRALLTCTISSGTIGLIGQENVALTCLRRSERLISFIMKKLNPFDFPIQAYVEL 554

Query: 241 QVGILNTLDRLTATEFFSKFSLKKLSSENHFVDIGEKIDEDFLKKEPSAIIIEQLTKYNV 300
           Q  ILNTLD LT TE FSK SL+KLSSE+HF+D GE+IDE FL K+ SAIIIEQLTKYN+
Sbjct: 555 QAAILNTLDSLTTTELFSKCSLRKLSSESHFLDAGEEIDETFLNKDHSAIIIEQLTKYNM 614

Query: 301 LFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVGGVGNLIFMVI 360
           LFSKAL+K+SPLTVK+TTLGWIQRFCENV+TIFKND TYANFFEAFGY G +GNLIFMVI
Sbjct: 615 LFSKALHKASPLTVKITTLGWIQRFCENVVTIFKNDTTYANFFEAFGYFGVIGNLIFMVI 674

Query: 361 DAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDIKNSFVRLLSH 420
           DAASDREPKVRSNAASVLELLL A+IVHP+YFYPIAD+VLEKLGDPD+DIKNSFVRLLSH
Sbjct: 675 DAASDREPKVRSNAASVLELLLQAKIVHPIYFYPIADIVLEKLGDPDNDIKNSFVRLLSH 734

Query: 421 VLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 480
           +LPTALYACG YD+G YPA R HLL SDHK SLHWKQVFALKQLPQQIHFQQLISILSYI
Sbjct: 735 ILPTALYACGQYDLGSYPASRLHLLRSDHKCSLHWKQVFALKQLPQQIHFQQLISILSYI 794

Query: 481 SQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDEFFYGNCSVNC 540
           SQRWKVPVASW QRLIHRCGRLKDID SQSEETGN GANGLWLD KVDDEF  GNCSVNC
Sbjct: 795 SQRWKVPVASWTQRLIHRCGRLKDIDSSQSEETGNFGANGLWLDLKVDDEFLNGNCSVNC 854

Query: 541 VAGLWWAIHEAARYCIALRLRTNLGGPTQ 570
           VAG+WWAIHEAARYCI LRLRTNLGGPTQ
Sbjct: 855 VAGVWWAIHEAARYCITLRLRTNLGGPTQ 883

[Close]

BLAST of Sgr021215 vs. NCBI nr
Match: XP_022944490.1 (serine/threonine-protein kinase SMG1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1013.1 bits (2618), Expect = 9.7e-292
Identity = 499/569 (87.70%), Postives = 529/569 (92.97%), Query Frame = 0


Query: 1   MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKA 60
           MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRE FSTFYPLAIDILFQ+LEMT+A
Sbjct: 315 MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRERFSTFYPLAIDILFQNLEMTRA 374

Query: 61  NRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTG 120
           N VVR  KITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLR+HPNHLVTG
Sbjct: 375 NHVVRGHKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRLHPNHLVTG 434

Query: 121 SSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLS 180
           SSAATYIFLLQHGNNEVVEQTVTLL EEL++FKGLLEKG+DQG++NG+L SQFYS +DL 
Sbjct: 435 SSAATYIFLLQHGNNEVVEQTVTLLTEELKVFKGLLEKGLDQGNINGILKSQFYSKMDLF 494

Query: 181 ALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVEL 240
           ALIKFDLR LLTCTIS GT+GLIGQE VA  CL+RSERLISFIMKKLNPFDFPVQAYVEL
Sbjct: 495 ALIKFDLRALLTCTISCGTIGLIGQENVALTCLRRSERLISFIMKKLNPFDFPVQAYVEL 554

Query: 241 QVGILNTLDRLTATEFFSKFSLKKLSSENHFVDIGEKIDEDFLKKEPSAIIIEQLTKYNV 300
           Q  ILNTLD LT TE FSK SL+KLSSE+HF+D GE+IDE FL K+ SAIIIEQLTKYN+
Sbjct: 555 QAAILNTLDSLTTTELFSKCSLRKLSSESHFLDAGEEIDETFLNKDHSAIIIEQLTKYNM 614

Query: 301 LFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVGGVGNLIFMVI 360
           LFSKAL+K+SPLTVK+TTLGWIQRFCENV+TIFKND TYANFFEAFGY G +GNLIFMVI
Sbjct: 615 LFSKALHKASPLTVKITTLGWIQRFCENVVTIFKNDTTYANFFEAFGYFGVIGNLIFMVI 674

Query: 361 DAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDIKNSFVRLLSH 420
           DAASDREPKVRSNAASVLELLL A+IVHP+YFYPIAD+VLEKLGDPD+DIKNSFVRLLSH
Sbjct: 675 DAASDREPKVRSNAASVLELLLQAKIVHPIYFYPIADIVLEKLGDPDNDIKNSFVRLLSH 734

Query: 421 VLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 480
           +LPTALYACG YD+G YPA R HLL SDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI
Sbjct: 735 ILPTALYACGQYDLGSYPASRLHLLRSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 794

Query: 481 SQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDEFFYGNCSVNC 540
           SQRWKVPVASW QRLIHRCGRLKDID SQSEETGN G NGLWLD KVDDEF  GNCSVNC
Sbjct: 795 SQRWKVPVASWTQRLIHRCGRLKDIDSSQSEETGNFGTNGLWLDLKVDDEFLNGNCSVNC 854

Query: 541 VAGLWWAIHEAARYCIALRLRTNLGGPTQ 570
           VAG+WWAIHEAARYCI LRLRTNLGGPTQ
Sbjct: 855 VAGVWWAIHEAARYCITLRLRTNLGGPTQ 883

[Close]

BLAST of Sgr021215 vs. NCBI nr
Match: XP_022944491.1 (serine/threonine-protein kinase SMG1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1013.1 bits (2618), Expect = 9.7e-292
Identity = 499/569 (87.70%), Postives = 529/569 (92.97%), Query Frame = 0


Query: 1   MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKA 60
           MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRE FSTFYPLAIDILFQ+LEMT+A
Sbjct: 315 MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRERFSTFYPLAIDILFQNLEMTRA 374

Query: 61  NRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTG 120
           N VVR  KITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLR+HPNHLVTG
Sbjct: 375 NHVVRGHKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRLHPNHLVTG 434

Query: 121 SSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLS 180
           SSAATYIFLLQHGNNEVVEQTVTLL EEL++FKGLLEKG+DQG++NG+L SQFYS +DL 
Sbjct: 435 SSAATYIFLLQHGNNEVVEQTVTLLTEELKVFKGLLEKGLDQGNINGILKSQFYSKMDLF 494

Query: 181 ALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVEL 240
           ALIKFDLR LLTCTIS GT+GLIGQE VA  CL+RSERLISFIMKKLNPFDFPVQAYVEL
Sbjct: 495 ALIKFDLRALLTCTISCGTIGLIGQENVALTCLRRSERLISFIMKKLNPFDFPVQAYVEL 554

Query: 241 QVGILNTLDRLTATEFFSKFSLKKLSSENHFVDIGEKIDEDFLKKEPSAIIIEQLTKYNV 300
           Q  ILNTLD LT TE FSK SL+KLSSE+HF+D GE+IDE FL K+ SAIIIEQLTKYN+
Sbjct: 555 QAAILNTLDSLTTTELFSKCSLRKLSSESHFLDAGEEIDETFLNKDHSAIIIEQLTKYNM 614

Query: 301 LFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVGGVGNLIFMVI 360
           LFSKAL+K+SPLTVK+TTLGWIQRFCENV+TIFKND TYANFFEAFGY G +GNLIFMVI
Sbjct: 615 LFSKALHKASPLTVKITTLGWIQRFCENVVTIFKNDTTYANFFEAFGYFGVIGNLIFMVI 674

Query: 361 DAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDIKNSFVRLLSH 420
           DAASDREPKVRSNAASVLELLL A+IVHP+YFYPIAD+VLEKLGDPD+DIKNSFVRLLSH
Sbjct: 675 DAASDREPKVRSNAASVLELLLQAKIVHPIYFYPIADIVLEKLGDPDNDIKNSFVRLLSH 734

Query: 421 VLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 480
           +LPTALYACG YD+G YPA R HLL SDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI
Sbjct: 735 ILPTALYACGQYDLGSYPASRLHLLRSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 794

Query: 481 SQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDEFFYGNCSVNC 540
           SQRWKVPVASW QRLIHRCGRLKDID SQSEETGN G NGLWLD KVDDEF  GNCSVNC
Sbjct: 795 SQRWKVPVASWTQRLIHRCGRLKDIDSSQSEETGNFGTNGLWLDLKVDDEFLNGNCSVNC 854

Query: 541 VAGLWWAIHEAARYCIALRLRTNLGGPTQ 570
           VAG+WWAIHEAARYCI LRLRTNLGGPTQ
Sbjct: 855 VAGVWWAIHEAARYCITLRLRTNLGGPTQ 883

[Close]

BLAST of Sgr021215 vs. ExPASy TrEMBL
Match: A0A6J1D4B0 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111017174 PE=3 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 9.2e-296
Identity = 506/569 (88.93%), Postives = 540/569 (94.90%), Query Frame = 0


Query: 1   MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKA 60
           MLPQLLGCLSMVG KFGWLEWIENLWKCLTLLAEILRE FSTFYPLAIDIL QSLEMT+A
Sbjct: 315 MLPQLLGCLSMVGHKFGWLEWIENLWKCLTLLAEILRERFSTFYPLAIDILSQSLEMTRA 374

Query: 61  NRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTG 120
           N V+R QKITFLQVHGVLKTNLQLLSLQKLGLL SSVHRILQFDAPISQLR+HPNHLVTG
Sbjct: 375 NHVMRGQKITFLQVHGVLKTNLQLLSLQKLGLLSSSVHRILQFDAPISQLRLHPNHLVTG 434

Query: 121 SSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLS 180
           S+AATYIFLLQH NNEVVE+T TLL+EELEMFKGLLEKG+DQGDVN ++DSQ YSNIDL 
Sbjct: 435 SAAATYIFLLQHRNNEVVEKTATLLMEELEMFKGLLEKGLDQGDVNVIIDSQIYSNIDLF 494

Query: 181 ALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVEL 240
           AL+KFDL+VLLTCTISSGT+GLIGQE VAF+CL RSERLISFIM KLNPFDFP+QAYVEL
Sbjct: 495 ALVKFDLKVLLTCTISSGTIGLIGQENVAFSCLTRSERLISFIMNKLNPFDFPIQAYVEL 554

Query: 241 QVGILNTLDRLTATEFFSKFSLKKLSSENHFVDIGEKIDEDFLKKEPSAIIIEQLTKYNV 300
           QVGIL+TLDRLT TE FSK+SLKKLSSENHFVDIG+K+DEDFLKK+ SAIIIEQLTKYN 
Sbjct: 555 QVGILDTLDRLTTTELFSKWSLKKLSSENHFVDIGKKVDEDFLKKDHSAIIIEQLTKYNT 614

Query: 301 LFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVGGVGNLIFMVI 360
           LFSKAL+KSSPLTVK+TTL WIQRFCENVI IF NDKTYANF+EAFGYVG VGNLIFMVI
Sbjct: 615 LFSKALHKSSPLTVKITTLAWIQRFCENVIIIFNNDKTYANFYEAFGYVGVVGNLIFMVI 674

Query: 361 DAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDIKNSFVRLLSH 420
           DAASDREPKVRSNAASVLELL+LA+IVHP++FYP+AD+VLEKLGDPD+DIKNSFVRL+SH
Sbjct: 675 DAASDREPKVRSNAASVLELLMLAKIVHPIHFYPVADIVLEKLGDPDNDIKNSFVRLVSH 734

Query: 421 VLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 480
           VLPTALYACGLY+VG YP  RP LLMSDH+SSLHWKQVFALKQLPQQI+FQQLISILSYI
Sbjct: 735 VLPTALYACGLYNVGAYPTSRP-LLMSDHRSSLHWKQVFALKQLPQQIYFQQLISILSYI 794

Query: 481 SQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDEFFYGNCSVNC 540
           SQRWKVPVASW QRLIHRCGR KDIDLSQSEETGNLGANGLWLDFKVDDEF YGNCSVNC
Sbjct: 795 SQRWKVPVASWTQRLIHRCGRWKDIDLSQSEETGNLGANGLWLDFKVDDEFLYGNCSVNC 854

Query: 541 VAGLWWAIHEAARYCIALRLRTNLGGPTQ 570
           VAG+WWAIHEAARYCIALRLRTNLGGPTQ
Sbjct: 855 VAGIWWAIHEAARYCIALRLRTNLGGPTQ 882

[Close]

BLAST of Sgr021215 vs. ExPASy TrEMBL
Match: A0A6J1JE22 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111484183 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 8.6e-294
Identity = 501/569 (88.05%), Postives = 532/569 (93.50%), Query Frame = 0


Query: 1   MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKA 60
           MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRE FSTFYPLAIDILFQ+LEMT+A
Sbjct: 315 MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRERFSTFYPLAIDILFQNLEMTRA 374

Query: 61  NRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTG 120
           N VVR  KITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLR+HPNHLVTG
Sbjct: 375 NHVVRGHKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRLHPNHLVTG 434

Query: 121 SSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLS 180
           SSAATYIFLLQHGNNEVVEQTVTLL EELE+FKGLLEKG+DQG++NG+L+SQFYS +DL 
Sbjct: 435 SSAATYIFLLQHGNNEVVEQTVTLLTEELEVFKGLLEKGLDQGNINGILESQFYSKMDLF 494

Query: 181 ALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVEL 240
           ALIKFDLR LLTCTISSGT+GLIGQE VA  CL+RSERLISFIMKKLNPFDFP+QAYVEL
Sbjct: 495 ALIKFDLRALLTCTISSGTIGLIGQENVALTCLRRSERLISFIMKKLNPFDFPIQAYVEL 554

Query: 241 QVGILNTLDRLTATEFFSKFSLKKLSSENHFVDIGEKIDEDFLKKEPSAIIIEQLTKYNV 300
           Q  ILNTLD LT TE FSK SL+KLSSE+HF+D GE+IDE FL K+ SAIIIEQLTKYN+
Sbjct: 555 QAAILNTLDSLTTTELFSKCSLRKLSSESHFLDAGEEIDETFLNKDHSAIIIEQLTKYNM 614

Query: 301 LFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVGGVGNLIFMVI 360
           LFSKAL+K+SPLTVK+TTLGWIQRFCENV+TIFKND TYANFFEAFGY G +GNLIFMVI
Sbjct: 615 LFSKALHKASPLTVKITTLGWIQRFCENVVTIFKNDTTYANFFEAFGYFGVIGNLIFMVI 674

Query: 361 DAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDIKNSFVRLLSH 420
           DAASDREPKVRSNAASVLELLL A+IVHP+YFYPIAD+VLEKLGDPD+DIKNSFVRLLSH
Sbjct: 675 DAASDREPKVRSNAASVLELLLQAKIVHPIYFYPIADIVLEKLGDPDNDIKNSFVRLLSH 734

Query: 421 VLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 480
           +LPTALYACG YD+G YPA R HLL SDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI
Sbjct: 735 ILPTALYACGQYDLGSYPASRLHLLRSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 794

Query: 481 SQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDEFFYGNCSVNC 540
           SQRWKVPVASW QRLIHRCGRLKDID SQSEETGN GANGLWLD KVDDEF  GNCSVNC
Sbjct: 795 SQRWKVPVASWTQRLIHRCGRLKDIDSSQSEETGNFGANGLWLDLKVDDEFLNGNCSVNC 854

Query: 541 VAGLWWAIHEAARYCIALRLRTNLGGPTQ 570
           VAG+WWAIHEAARYCI LRLRTNLGGPTQ
Sbjct: 855 VAGVWWAIHEAARYCITLRLRTNLGGPTQ 883

[Close]

BLAST of Sgr021215 vs. ExPASy TrEMBL
Match: A0A6J1FUJ9 (serine/threonine-protein kinase SMG1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448933 PE=3 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 4.7e-292
Identity = 499/569 (87.70%), Postives = 529/569 (92.97%), Query Frame = 0


Query: 1   MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKA 60
           MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRE FSTFYPLAIDILFQ+LEMT+A
Sbjct: 315 MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRERFSTFYPLAIDILFQNLEMTRA 374

Query: 61  NRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTG 120
           N VVR  KITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLR+HPNHLVTG
Sbjct: 375 NHVVRGHKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRLHPNHLVTG 434

Query: 121 SSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLS 180
           SSAATYIFLLQHGNNEVVEQTVTLL EEL++FKGLLEKG+DQG++NG+L SQFYS +DL 
Sbjct: 435 SSAATYIFLLQHGNNEVVEQTVTLLTEELKVFKGLLEKGLDQGNINGILKSQFYSKMDLF 494

Query: 181 ALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVEL 240
           ALIKFDLR LLTCTIS GT+GLIGQE VA  CL+RSERLISFIMKKLNPFDFPVQAYVEL
Sbjct: 495 ALIKFDLRALLTCTISCGTIGLIGQENVALTCLRRSERLISFIMKKLNPFDFPVQAYVEL 554

Query: 241 QVGILNTLDRLTATEFFSKFSLKKLSSENHFVDIGEKIDEDFLKKEPSAIIIEQLTKYNV 300
           Q  ILNTLD LT TE FSK SL+KLSSE+HF+D GE+IDE FL K+ SAIIIEQLTKYN+
Sbjct: 555 QAAILNTLDSLTTTELFSKCSLRKLSSESHFLDAGEEIDETFLNKDHSAIIIEQLTKYNM 614

Query: 301 LFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVGGVGNLIFMVI 360
           LFSKAL+K+SPLTVK+TTLGWIQRFCENV+TIFKND TYANFFEAFGY G +GNLIFMVI
Sbjct: 615 LFSKALHKASPLTVKITTLGWIQRFCENVVTIFKNDTTYANFFEAFGYFGVIGNLIFMVI 674

Query: 361 DAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDIKNSFVRLLSH 420
           DAASDREPKVRSNAASVLELLL A+IVHP+YFYPIAD+VLEKLGDPD+DIKNSFVRLLSH
Sbjct: 675 DAASDREPKVRSNAASVLELLLQAKIVHPIYFYPIADIVLEKLGDPDNDIKNSFVRLLSH 734

Query: 421 VLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 480
           +LPTALYACG YD+G YPA R HLL SDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI
Sbjct: 735 ILPTALYACGQYDLGSYPASRLHLLRSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 794

Query: 481 SQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDEFFYGNCSVNC 540
           SQRWKVPVASW QRLIHRCGRLKDID SQSEETGN G NGLWLD KVDDEF  GNCSVNC
Sbjct: 795 SQRWKVPVASWTQRLIHRCGRLKDIDSSQSEETGNFGTNGLWLDLKVDDEFLNGNCSVNC 854

Query: 541 VAGLWWAIHEAARYCIALRLRTNLGGPTQ 570
           VAG+WWAIHEAARYCI LRLRTNLGGPTQ
Sbjct: 855 VAGVWWAIHEAARYCITLRLRTNLGGPTQ 883

[Close]

BLAST of Sgr021215 vs. ExPASy TrEMBL
Match: A0A6J1FYP6 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111448933 PE=3 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 4.7e-292
Identity = 499/569 (87.70%), Postives = 529/569 (92.97%), Query Frame = 0


Query: 1   MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKA 60
           MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRE FSTFYPLAIDILFQ+LEMT+A
Sbjct: 315 MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILRERFSTFYPLAIDILFQNLEMTRA 374

Query: 61  NRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTG 120
           N VVR  KITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLR+HPNHLVTG
Sbjct: 375 NHVVRGHKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRLHPNHLVTG 434

Query: 121 SSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLS 180
           SSAATYIFLLQHGNNEVVEQTVTLL EEL++FKGLLEKG+DQG++NG+L SQFYS +DL 
Sbjct: 435 SSAATYIFLLQHGNNEVVEQTVTLLTEELKVFKGLLEKGLDQGNINGILKSQFYSKMDLF 494

Query: 181 ALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVEL 240
           ALIKFDLR LLTCTIS GT+GLIGQE VA  CL+RSERLISFIMKKLNPFDFPVQAYVEL
Sbjct: 495 ALIKFDLRALLTCTISCGTIGLIGQENVALTCLRRSERLISFIMKKLNPFDFPVQAYVEL 554

Query: 241 QVGILNTLDRLTATEFFSKFSLKKLSSENHFVDIGEKIDEDFLKKEPSAIIIEQLTKYNV 300
           Q  ILNTLD LT TE FSK SL+KLSSE+HF+D GE+IDE FL K+ SAIIIEQLTKYN+
Sbjct: 555 QAAILNTLDSLTTTELFSKCSLRKLSSESHFLDAGEEIDETFLNKDHSAIIIEQLTKYNM 614

Query: 301 LFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVGGVGNLIFMVI 360
           LFSKAL+K+SPLTVK+TTLGWIQRFCENV+TIFKND TYANFFEAFGY G +GNLIFMVI
Sbjct: 615 LFSKALHKASPLTVKITTLGWIQRFCENVVTIFKNDTTYANFFEAFGYFGVIGNLIFMVI 674

Query: 361 DAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDIKNSFVRLLSH 420
           DAASDREPKVRSNAASVLELLL A+IVHP+YFYPIAD+VLEKLGDPD+DIKNSFVRLLSH
Sbjct: 675 DAASDREPKVRSNAASVLELLLQAKIVHPIYFYPIADIVLEKLGDPDNDIKNSFVRLLSH 734

Query: 421 VLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 480
           +LPTALYACG YD+G YPA R HLL SDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI
Sbjct: 735 ILPTALYACGQYDLGSYPASRLHLLRSDHKSSLHWKQVFALKQLPQQIHFQQLISILSYI 794

Query: 481 SQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDEFFYGNCSVNC 540
           SQRWKVPVASW QRLIHRCGRLKDID SQSEETGN G NGLWLD KVDDEF  GNCSVNC
Sbjct: 795 SQRWKVPVASWTQRLIHRCGRLKDIDSSQSEETGNFGTNGLWLDLKVDDEFLNGNCSVNC 854

Query: 541 VAGLWWAIHEAARYCIALRLRTNLGGPTQ 570
           VAG+WWAIHEAARYCI LRLRTNLGGPTQ
Sbjct: 855 VAGVWWAIHEAARYCITLRLRTNLGGPTQ 883

[Close]

BLAST of Sgr021215 vs. ExPASy TrEMBL
Match: A0A0A0LLV1 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_2G237710 PE=3 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.1e-288
Identity = 499/579 (86.18%), Postives = 529/579 (91.36%), Query Frame = 0


Query: 1   MLPQLLGCLSMVGRKFGWLEWIENLWKCLTLLAEILREHFSTFYPLAIDILFQSLEMTKA 60
           MLPQLLGCLSMVGRKFGWLEWI+NLWKCLTLLAEILRE FST+YPLAIDILFQSLEMT+A
Sbjct: 315 MLPQLLGCLSMVGRKFGWLEWIDNLWKCLTLLAEILRERFSTYYPLAIDILFQSLEMTRA 374

Query: 61  NRVVRRQKITFLQVHGVLKTNLQLLSLQKLGLLPSSVHRILQFDAPISQLRMHPNHLVTG 120
           NRVV+ QKITFLQVHGVLKTNLQLLSLQK GLLPSSVHRILQFDAPISQLRMHPNHLVTG
Sbjct: 375 NRVVKGQKITFLQVHGVLKTNLQLLSLQKFGLLPSSVHRILQFDAPISQLRMHPNHLVTG 434

Query: 121 SSAATYIFLLQHGNNEVVEQTVTLLIEELEMFKGLLEKGIDQGDVNGVLDSQFYSNIDLS 180
           SSAATYIFLLQHGNNEVVEQTV LLIEEL MF GLLEKG+DQ  +NG+LDSQF S +DL 
Sbjct: 435 SSAATYIFLLQHGNNEVVEQTVALLIEELGMFSGLLEKGLDQRGINGILDSQFCSTMDLF 494

Query: 181 ALIKFDLRVLLTCTISSGTVGLIGQEKVAFACLKRSERLISFIMKKLNPFDFPVQAYVEL 240
           ALIKFDLR LLTCTISSGT+GLIGQE VAF CLKRSERLISFIM+KLNPFDFP+QAYVEL
Sbjct: 495 ALIKFDLRALLTCTISSGTIGLIGQENVAFTCLKRSERLISFIMEKLNPFDFPLQAYVEL 554

Query: 241 QVGILNTLDRLTATEFFSKFSLKKLSSENHFVDI----------GEKIDEDFLKKEPSAI 300
           Q  IL+TLDRLT TEFF K SLKKLSSEN F+D           GE IDE  LKK+ SAI
Sbjct: 555 QAAILDTLDRLTTTEFFCKCSLKKLSSENRFLDSGENIDSYQKKGENIDEAHLKKDHSAI 614

Query: 301 IIEQLTKYNVLFSKALNKSSPLTVKVTTLGWIQRFCENVITIFKNDKTYANFFEAFGYVG 360
           IIEQLTKYN LFSKAL+K+SPLTVK+TTLGWIQRFCENV+TIFKNDKTYANFFE FGY  
Sbjct: 615 IIEQLTKYNALFSKALHKASPLTVKITTLGWIQRFCENVVTIFKNDKTYANFFEEFGYFS 674

Query: 361 GVGNLIFMVIDAASDREPKVRSNAASVLELLLLARIVHPMYFYPIADVVLEKLGDPDDDI 420
            +GNLIFMVIDAASDREPKVRSNAASVLELLL A+IVHP+YFYPIADVVLEKLGDPD++I
Sbjct: 675 VIGNLIFMVIDAASDREPKVRSNAASVLELLLQAKIVHPIYFYPIADVVLEKLGDPDNEI 734

Query: 421 KNSFVRLLSHVLPTALYACGLYDVGVYPACRPHLLMSDHKSSLHWKQVFALKQLPQQIHF 480
           KNSFVRLLSH+LPTALYACG YD+G YPACR HLL SDHKSSLHWKQVFALKQLPQQIHF
Sbjct: 735 KNSFVRLLSHILPTALYACGQYDLGSYPACRLHLLRSDHKSSLHWKQVFALKQLPQQIHF 794

Query: 481 QQLISILSYISQRWKVPVASWMQRLIHRCGRLKDIDLSQSEETGNLGANGLWLDFKVDDE 540
           QQLISILSYISQRWKVPVASW QRLIHRCGRLKDIDLSQSEE GNLGANGLWLD ++DD+
Sbjct: 795 QQLISILSYISQRWKVPVASWTQRLIHRCGRLKDIDLSQSEEMGNLGANGLWLDLRLDDD 854

Query: 541 FFYGNCSVNCVAGLWWAIHEAARYCIALRLRTNLGGPTQ 570
           F  GNCSVNCVAG+WWAIHEAARYCI+LRLRTNLGGPTQ
Sbjct: 855 FLNGNCSVNCVAGVWWAIHEAARYCISLRLRTNLGGPTQ 893

The following BLAST results are available for this feature:

BLAST of Sgr021215 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148544.1	1.9e-295	88.93	serine/threonine-protein kinase SMG1-like [Momordica charantia]	[more]
XP_022986435.1	1.8e-293	88.05	serine/threonine-protein kinase SMG1-like [Cucurbita maxima]	[more]
XP_023513297.1	7.5e-292	87.70	serine/threonine-protein kinase SMG1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022944490.1	9.7e-292	87.70	serine/threonine-protein kinase SMG1-like isoform X1 [Cucurbita moschata]	[more]
XP_022944491.1	9.7e-292	87.70	serine/threonine-protein kinase SMG1-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Sgr021215 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Sgr021215 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D4B0	9.2e-296	88.93	Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1JE22	8.6e-294	88.05	Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1FUJ9	4.7e-292	87.70	serine/threonine-protein kinase SMG1-like isoform X2 OS=Cucurbita moschata OX=36...	[more]
A0A6J1FYP6	4.7e-292	87.70	Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A0A0LLV1	1.1e-288	86.18	Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_2...	[more]

BLAST of Sgr021215 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031559	Serine/threonine-protein kinase SMG1	PFAM	PF15785	SMG1	coord: 301..568 e-value: 1.5E-8 score: 33.5
None	No IPR available	PANTHER	PTHR11139	ATAXIA TELANGIECTASIA MUTATED ATM -RELATED	coord: 1..569
None	No IPR available	PANTHER	PTHR11139:SF71	OS03G0738200 PROTEIN	coord: 1..569
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 27..478

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr021215.1	Sgr021215.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000184	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0106310	protein serine kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004712	protein serine/threonine/tyrosine kinase activity