Sgr021106 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr021106
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionCytochrome P450, putative
Locationtig00153640: 605595 .. 609239 (+)
RNA-Seq ExpressionSgr021106
SyntenySgr021106
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTGTGATTCTTCTAACGACGCTGCTGGTGATTTTAGTTTCATCCCTCGCAAAGATTGGGTACGATACAATCTCATGCTACTGGCTGACGCCTAGGCGCATCAGGAAGAGGATGGAGAAGCAAGGCCTGCGGGGGCCGGAGCCCCGGCCGCTGATCGGAAACATCCTCGACGTAGCGGCGCTGGTGGCGAAATCCACCGCCAACGACATGAGCGGCATCGATCACGATATCGTCGGCCGTCTGTTGCCTCATTACATAGCATGGACAAAGCAGTACGGTGCGTGTAATGGACGGTTATTTTTTTTTATTATTAGAAAAATTTCTAGTATTCTCGTAGTACCTAAAAGGTAGTAGTGGGGGTAATTTTATAGGGAAGAGGTTTATGTACTGGAACGGAACGGAGCCGAGGCTGTGTTTGGCAGAAACGGAGTTGATTAAAGAGTTGCTGTCGAAAAATAGCAGCGTGTGTGGGAAATCATGGCTGCAAAGACAAGGAACCAAACACTTCATAGGGAAAGGATTGTTAATGGCGAATGGTGAAGATTGGTATCACCAACGTCACATCGTTGCGCCTGCGTTTATGGGGGACAAGCTCAAGGTACATATTAAAAATAATAATAATAAACATTACCTCCTCACATGTTCTATTCTTCTTCTTATTATGATTGTTATTCAACTTTATATTACAAGTTTAGTCTCGATGATGTTCGGCCCATTTTTCGTAATGTTTTTTGATTGTTGTTTTTTTTTTTTTTTTGTAAGAAAGAAAGAAGGGATGTGGATGTGTTTGGGAATTATTTCTTATTTTTGTAATTAAAAAAAGGAATATGAATATGTTTTGTTCTCTCAAATTTTAAACTAAAAAAATTAAAGTTTTACTTTGAAAAAAATAAAAAATATTCAAAATTATTAAGTTATATTAATTTTTTTTATATTTACGATGGATTTTATAAACTAAAAATAATATAATAATCCTGTTACATGTGTTATGATTCCAAAAAAAAAAAAGGAGAAACACAACAAAAACCCATTAACAACAGACTATATTTCTCAAAAATTCAAAAACAAGAAACGAAAAACGAGAAATCAAAACGTACCAAAACGGTCTCAAAATTTTAATGAGTTAGGTTTATTTAATCGCTAAAATTTTAAAATATTTCAATTACATCCCTAAACTATGATTCTATATACATAGTTGCTATTACTATTAATATAGTATGCTAAAGAACAACATAACATGCCTATTAAACAAATGTCGACTAATTTAGTACACCACCTCCTAGAATTCATGTAGGGTAAAATAAATGTAGAATCGACAAACTCGAACATTTCCAAGCGATGGCTGAATTGACAAAGTTTAAATCAAAATTCTTCCGTTGGTACTTTTTGTCTAATAAAAAGAGAAAAGATAGGACCCATATGTATAAACAATGTTAGTTAGCCGGTGAAGAAATTTTTTTTTATTATATATATATTTTTTGGGGTTTTTCCATTTTTATGTAATGTGTTTTTTTTCATATTGTAATTATATTTTTGGATTTGTTTTGTTTGTATTGACATCAGCAGAGTTATGCGGGGTACATGGTGGAGTGCACCAAGAACATGATTCAGTCACTCCAGACCGAGTTGAACTCGGGCCGGACCGAGTTCGAGATCGCCGAGTTCATGAAGCAACTCACCGCCGACATCATCTCCCGGACGGAGTTCGACAGCAGCTACGACAAAGGGAAGCAAATATTCCATTTGCTCACTCTTCTCCAGCGCCTCTGCGCCCAAGCCAGCCGCCACCTCTGCCTCCCCGGCAGCCGGTAAGTACCCGTTTAACGTTCTCGATTCTTATTTCTTGTCCTCTTTCTTTTTTTTGTTTTTTAATTTTTTAAAAGGAAAAATATATTTGATAACAATTTTTTTTTTCTCAAATTTGAAGTCTAAAAAGATTCAAATTTAACATGAAAATTAATTTTTTAAAATAAAATAATCGTGTTATATTTTAATTTATTTTTAAAAAACTACAATTAATAAATTAGATTTTTGTTGATTCTAAAATTTGTATACAATCTTTTAATCTGAGTATAGTTTAAGACAAGACACTTATTACTTTTTTAGATTGAAGTTTCGATCTCTCACCCTTACATTTTTTAAACTAAAAAAATAATGTATACAATTTTAAGAATGTTTCTCAAAAAAGAGAAATGAGAAACAAGAAACAGTAATGTTACCAAACGAGTCCTAATCATCGTGTTTCGGGTTTCTTCCATTGCTCAAGATGATGAAGTTGCAGTGGGTTTTTTGGTGCAGGTTTTTTCCGAGCAAGTACAACAGAGAAATAAAGTCGCTGAAAATGGAGGTGGAGAGGTTGCTAATGCAGATTATTCAGAGCAGGAAAGATGGCGTGGAGATTGGGAGAAGCACTTGCTATGGAAATGACTTGCTGGGAATGCTGTTGAAAGAGATGCAGAGGAAGAGAGAAGATGGGTTCAGCTTGAACTTGCAGCTTATAATGGATGAATGCAAAACTTTCTTCTTCGCCGGCCATGAAACCACTGCTCTTCTCCTCACTTGGACCATCATGTTGCTGGCCAGTAACCCTACTTGGCAGGAGAAGGTTCGAGCTGAAGTGAAGCAGGTCTGCAATGGCGGAACTCCCTCTGTTGAGCATCTCTCTAAGCTCACTCTCGTAAGCAAACTAAAACTTCGATTCTCTAAGAATATATGTAAGAGATTAAATATACATGAACCCATTAGCTTGACTTTTTAGGGTTTAGTGGTGATTTAACATGATATTAAGAGATCGTGAGTTTGAGTTGTCTGAGCAATGTCATTTTCATCCTAATTGGTATTAATAATTCACGTGTTGGACTTCATGAATCAAATTAATATCTGAGTCCACCAAAGAGAGGCGTTAAGAATATATATAAAAAAAGATTAAATGTATCCCTTTGTGTTAGTACTCTAGGCCTTTAGGGTTTTGTGGTCATCTAGCACTATGTTATTAGATTAGGAGGTCTTGAGTTTGAATCGTTGGAAAGTCATTTCTTCTCTAATGGATATTAATAGTTCACGTGTTTGGTTTCGTGAATCAAATTTATATCTAAACCCTAAATATATTTTATAACCTATCAACTTACACTTTTAGATTTAGGGACATGATATTAAAATAAGAGAGATGTTGAGTAAATATATATATATATATATTTGATATAGGATATGTTTTTGTAGCTAAACATGGTGATAAACGAATCCCTAAGGCTTTACCCACCGGCCACGGTGCTTCCAAGAATGGCGTTCGAAGACATAAAGCTAGGGGATTTAGAGATCCCGAAAGGGCTATCGATATGGATCCCGGTGCTGGCGATTCATCACAGCGAAGAATTGTGGGGAAAAGATGCAAACGAGTTCAACCCAGAAAGATTTGCCTCAAAATCATTCAGTGCAGGGCGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGCCAAGCTTTTGCAATGATGGAAGCTAAGATCATATTGGCACTGTTCGTCTCCAACTTTAGCTTCACAATTTCAGATACTTATCGCCATGCTCCTGTTGTTGTTCTCACCATACAGCCTAAGCATGGAGTTCAAGTTTGCTTGACCCCATTGAATTCTTGA

mRNA sequence

ATGGCGGCTGTGATTCTTCTAACGACGCTGCTGGTGATTTTAGTTTCATCCCTCGCAAAGATTGGGTACGATACAATCTCATGCTACTGGCTGACGCCTAGGCGCATCAGGAAGAGGATGGAGAAGCAAGGCCTGCGGGGGCCGGAGCCCCGGCCGCTGATCGGAAACATCCTCGACGTAGCGGCGCTGGTGGCGAAATCCACCGCCAACGACATGAGCGGCATCGATCACGATATCGTCGGCCGTCTGTTGCCTCATTACATAGCATGGACAAAGCAGTACGGGAAGAGGTTTATGTACTGGAACGGAACGGAGCCGAGGCTGTGTTTGGCAGAAACGGAGTTGATTAAAGAGTTGCTGTCGAAAAATAGCAGCGTGTGTGGGAAATCATGGCTGCAAAGACAAGGAACCAAACACTTCATAGGGAAAGGATTGTTAATGGCGAATGGTGAAGATTGGTATCACCAACGTCACATCGTTGCGCCTGCGTTTATGGGGGACAAGCTCAAGAGTTATGCGGGGTACATGGTGGAGTGCACCAAGAACATGATTCAGTCACTCCAGACCGAGTTGAACTCGGGCCGGACCGAGTTCGAGATCGCCGAGTTCATGAAGCAACTCACCGCCGACATCATCTCCCGGACGGAGTTCGACAGCAGCTACGACAAAGGGAAGCAAATATTCCATTTGCTCACTCTTCTCCAGCGCCTCTGCGCCCAAGCCAGCCGCCACCTCTGCCTCCCCGGCAGCCGGTTTTTTCCGAGCAAGTACAACAGAGAAATAAAGTCGCTGAAAATGGAGGTGGAGAGGTTGCTAATGCAGATTATTCAGAGCAGGAAAGATGGCGTGGAGATTGGGAGAAGCACTTGCTATGGAAATGACTTGCTGGGAATGCTGTTGAAAGAGATGCAGAGGAAGAGAGAAGATGGGTTCAGCTTGAACTTGCAGCTTATAATGGATGAATGCAAAACTTTCTTCTTCGCCGGCCATGAAACCACTGCTCTTCTCCTCACTTGGACCATCATGTTGCTGGCCAGTAACCCTACTTGGCAGGAGAAGGTTCGAGCTGAAGTGAAGCAGGTCTGCAATGGCGGAACTCCCTCTGTTGAGCATCTCTCTAAGCTCACTCTCCTAAACATGGTGATAAACGAATCCCTAAGGCTTTACCCACCGGCCACGGTGCTTCCAAGAATGGCGTTCGAAGACATAAAGCTAGGGGATTTAGAGATCCCGAAAGGGCTATCGATATGGATCCCGGTGCTGGCGATTCATCACAGCGAAGAATTGTGGGGAAAAGATGCAAACGAGTTCAACCCAGAAAGATTTGCCTCAAAATCATTCAGTGCAGGGCGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGCCAAGCTTTTGCAATGATGGAAGCTAAGATCATATTGGCACTGTTCGTCTCCAACTTTAGCTTCACAATTTCAGATACTTATCGCCATGCTCCTGTTGTTGTTCTCACCATACAGCCTAAGCATGGAGTTCAAGTTTGCTTGACCCCATTGAATTCTTGA

Coding sequence (CDS)

ATGGCGGCTGTGATTCTTCTAACGACGCTGCTGGTGATTTTAGTTTCATCCCTCGCAAAGATTGGGTACGATACAATCTCATGCTACTGGCTGACGCCTAGGCGCATCAGGAAGAGGATGGAGAAGCAAGGCCTGCGGGGGCCGGAGCCCCGGCCGCTGATCGGAAACATCCTCGACGTAGCGGCGCTGGTGGCGAAATCCACCGCCAACGACATGAGCGGCATCGATCACGATATCGTCGGCCGTCTGTTGCCTCATTACATAGCATGGACAAAGCAGTACGGGAAGAGGTTTATGTACTGGAACGGAACGGAGCCGAGGCTGTGTTTGGCAGAAACGGAGTTGATTAAAGAGTTGCTGTCGAAAAATAGCAGCGTGTGTGGGAAATCATGGCTGCAAAGACAAGGAACCAAACACTTCATAGGGAAAGGATTGTTAATGGCGAATGGTGAAGATTGGTATCACCAACGTCACATCGTTGCGCCTGCGTTTATGGGGGACAAGCTCAAGAGTTATGCGGGGTACATGGTGGAGTGCACCAAGAACATGATTCAGTCACTCCAGACCGAGTTGAACTCGGGCCGGACCGAGTTCGAGATCGCCGAGTTCATGAAGCAACTCACCGCCGACATCATCTCCCGGACGGAGTTCGACAGCAGCTACGACAAAGGGAAGCAAATATTCCATTTGCTCACTCTTCTCCAGCGCCTCTGCGCCCAAGCCAGCCGCCACCTCTGCCTCCCCGGCAGCCGGTTTTTTCCGAGCAAGTACAACAGAGAAATAAAGTCGCTGAAAATGGAGGTGGAGAGGTTGCTAATGCAGATTATTCAGAGCAGGAAAGATGGCGTGGAGATTGGGAGAAGCACTTGCTATGGAAATGACTTGCTGGGAATGCTGTTGAAAGAGATGCAGAGGAAGAGAGAAGATGGGTTCAGCTTGAACTTGCAGCTTATAATGGATGAATGCAAAACTTTCTTCTTCGCCGGCCATGAAACCACTGCTCTTCTCCTCACTTGGACCATCATGTTGCTGGCCAGTAACCCTACTTGGCAGGAGAAGGTTCGAGCTGAAGTGAAGCAGGTCTGCAATGGCGGAACTCCCTCTGTTGAGCATCTCTCTAAGCTCACTCTCCTAAACATGGTGATAAACGAATCCCTAAGGCTTTACCCACCGGCCACGGTGCTTCCAAGAATGGCGTTCGAAGACATAAAGCTAGGGGATTTAGAGATCCCGAAAGGGCTATCGATATGGATCCCGGTGCTGGCGATTCATCACAGCGAAGAATTGTGGGGAAAAGATGCAAACGAGTTCAACCCAGAAAGATTTGCCTCAAAATCATTCAGTGCAGGGCGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGCCAAGCTTTTGCAATGATGGAAGCTAAGATCATATTGGCACTGTTCGTCTCCAACTTTAGCTTCACAATTTCAGATACTTATCGCCATGCTCCTGTTGTTGTTCTCACCATACAGCCTAAGCATGGAGTTCAAGTTTGCTTGACCCCATTGAATTCTTGA

Protein sequence

MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDVAALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELLSKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECTKNMIQSLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLLKEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVKQVCNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIILALFVSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS
Homology
BLAST of Sgr021106 vs. NCBI nr
Match: XP_038905687.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 941.0 bits (2431), Expect = 4.2e-270
Identity = 461/518 (89.00%), Postives = 491/518 (94.79%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           MA VI LTT+LVI +SSLAKI YDTISCYWLTPRRIRK+MEKQG+ GP+PRPL+GNILDV
Sbjct: 1   MAIVIFLTTILVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVCGPDPRPLLGNILDV 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
           AALVAKSTANDM  IDH+IVGRLLPHY  WTKQYGKRF+YWNGTEPRLCLAETELIKE+L
Sbjct: 61  AALVAKSTANDMKSIDHNIVGRLLPHYTTWTKQYGKRFIYWNGTEPRLCLAETELIKEVL 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           SKNS+VCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAG MVECT
Sbjct: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGLMVECT 180

Query: 181 KNMIQSLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQ 240
           K MIQSL+TE +SGRTEFEIA++MKQLTADIISRTEFD++ DKGK+IFHLLTLLQRLCAQ
Sbjct: 181 KKMIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLM+IIQSRKDGVEIGRSTCYG+DLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 KEMQRKRED-GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVK 360
            EMQRKRED GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLA+NP WQ KVR EV 
Sbjct: 301 NEMQRKREDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPDWQHKVRHEVN 360

Query: 361 QVC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWI 420
           QVC NGG+PSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWI
Sbjct: 361 QVCNNGGSPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWI 420

Query: 421 PVLAIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIILAL 480
           PVLAIHHSEELWG+DANEF PERFASK FS GRFIPFA+GPRNCIGQ FA+MEAKIILA+
Sbjct: 421 PVLAIHHSEELWGEDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKIILAM 480

Query: 481 FVSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
           F+S FSFTISD+YRHAPV VLTIQPK+GVQV LTP+NS
Sbjct: 481 FISKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 518

BLAST of Sgr021106 vs. NCBI nr
Match: XP_008461587.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 922.9 bits (2384), Expect = 1.2e-264
Identity = 451/520 (86.73%), Postives = 484/520 (93.08%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           MA +I LTTLLVI +SSLAKI YDTISCYWLTPRRIRK+MEKQG+RGP PRPL+GNILDV
Sbjct: 1   MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
           AAL+AKSTA DMS IDH+IV RLLPHY  WTKQYGKRF+YWNG EPRLCLAETELIKE+L
Sbjct: 61  AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           SKNS+VCG+SWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAP+FMGDKLKSYAG MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 KNMIQSLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQ 240
             +IQSL+TEL+SGRTEFEIA++MKQLTADIISRTEFD++ DKG QIFHLLTLLQ LCAQ
Sbjct: 181 DKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLM+IIQSRKDGVEIGRSTCYG+DLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 KEMQRKRED---GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAE 360
            EMQRK++D   GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLA+NP WQ+KVR E
Sbjct: 301 NEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VKQVC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSI 420
           V QVC NG TPSVEHLSK TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIIL 480
           WIPVLAIHHSEELWGKD NEF PERFASK FS GRFIPFAAGPRNC+GQ FA+MEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIIL 480

Query: 481 ALFVSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
           A+ +S FSFTISD+YRHAPV VLTIQPK+GVQV LTP+NS
Sbjct: 481 AMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Sgr021106 vs. NCBI nr
Match: XP_004145660.1 (cytokinin hydroxylase [Cucumis sativus] >KGN57750.1 hypothetical protein Csa_009800 [Cucumis sativus])

HSP 1 Score: 918.3 bits (2372), Expect = 2.9e-263
Identity = 449/520 (86.35%), Postives = 482/520 (92.69%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           MA +I+LTTLLVI +SSLAKI YDTISCYWLTPRRIRK+MEKQG+RGP PRPL+GNILDV
Sbjct: 1   MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
           AAL+ KSTA DMS IDH+IV RLLPHY  WTKQYGKRF+YWNG EPRLCLAETELIKE+L
Sbjct: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           SKNS+VCG+SWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAP+FMGDKLKSYAG MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 KNMIQSLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQ 240
             +IQSL+TE +SGRTEFEIA++MKQLTADIISRTEFD + DKG QIFHLLTLLQ LCAQ
Sbjct: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLM+IIQSRKDGVEIGRSTCYG+DLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 KEMQRKRED---GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAE 360
            EMQ++RED   GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLA+NP WQ+KVR E
Sbjct: 301 NEMQKRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VKQVC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSI 420
           V QVC NG TPSVEHLSK TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIIL 480
           WIPVLAIHHSEELWGKD NEF PERFASK FS GRFIPFAAGPRNCIGQ FA+MEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL 480

Query: 481 ALFVSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
           A+ +S FSF+ISD+YRHAPV VLTIQPK+GVQV LTP+NS
Sbjct: 481 AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Sgr021106 vs. NCBI nr
Match: XP_022983651.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 896.7 bits (2316), Expect = 9.2e-257
Identity = 438/517 (84.72%), Postives = 471/517 (91.10%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           MA +I LTTL VI +SSLAKI YDT S Y LTPRRIRK ME+QG+RGP+PR LIGNILDV
Sbjct: 1   MAFIIFLTTLFVIFISSLAKISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
             LVAKSTANDMS IDHDIV RLLPHY  WTKQYGKRF+YWNGTEPRLCLAETELIKELL
Sbjct: 61  VGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQYGKRFIYWNGTEPRLCLAETELIKELL 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
            K SSVCG+SWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAG+MVECT
Sbjct: 121 GKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGFMVECT 180

Query: 181 KNMIQSLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQ 240
             MIQS++TEL+SGRTEFEIA++MKQLTADIISRTEFD+  DKG QIFHLLTLLQRLCAQ
Sbjct: 181 NKMIQSMETELDSGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLL 300
           ASRHLCLPGSRF PSKYNREIKSLK EVERLLM+II+SRKDGVEIGRS+CYG+DLLGMLL
Sbjct: 241 ASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLL 300

Query: 301 KEM-QRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVK 360
            EM QRK     +LNLQLI+DECKTFFFAGHETTALLLTWTIMLLA+NP WQ  VRAEV 
Sbjct: 301 NEMQQRKNSTTTTLNLQLIIDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVN 360

Query: 361 QVCNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIP 420
           QVCN  TPS++HLSKLTLLNMVINESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIP
Sbjct: 361 QVCNARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIP 420

Query: 421 VLAIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIILALF 480
           VLAIHHSEELWGKDAN+FNP RFA+K+F  GRFIPFA GPRNCIGQ FA+MEAKIILA+F
Sbjct: 421 VLAIHHSEELWGKDANQFNPNRFAAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMF 480

Query: 481 VSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
           +S FSFTISDTYRHAPVVVLTIQPKHGVQVCLTP +S
Sbjct: 481 LSKFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPFSS 517

BLAST of Sgr021106 vs. NCBI nr
Match: PQQ00573.1 (cytokinin hydroxylase [Prunus yedoensis var. nudiflora])

HSP 1 Score: 892.1 bits (2304), Expect = 2.3e-255
Identity = 429/514 (83.46%), Postives = 478/514 (93.00%), Query Frame = 0

Query: 3   AVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDVAA 62
           A +LLTTLL+IL+S L ++ YDT+SCY+LTPRRI+K MEKQG+RGP+PRPL GNILD+A+
Sbjct: 2   AAVLLTTLLIILISLLIRVAYDTLSCYFLTPRRIKKIMEKQGVRGPKPRPLNGNILDMAS 61

Query: 63  LVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELLSK 122
           LVAKST++DM  I HDIVGRLLPHY+ W+KQYGKRF+YW G EPR+CLAETELIKELLSK
Sbjct: 62  LVAKSTSHDMQTISHDIVGRLLPHYVLWSKQYGKRFIYWTGIEPRMCLAETELIKELLSK 121

Query: 123 NSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECTKN 182
            S++ GKSWLQ+QG+KHFIG+GLLMANGEDWYHQRHIVAPAFMGD+LKSYAGYMVECTK 
Sbjct: 122 YSTISGKSWLQQQGSKHFIGRGLLMANGEDWYHQRHIVAPAFMGDRLKSYAGYMVECTKK 181

Query: 183 MIQSLQTEL-NSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQA 242
           M+QSLQ E+  SG  EFEI E+M +LTADIISRTEFDSSY+KGKQIF LLT+LQ LC+QA
Sbjct: 182 MLQSLQNEIETSGGREFEIGEYMTRLTADIISRTEFDSSYEKGKQIFRLLTVLQHLCSQA 241

Query: 243 SRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLLK 302
           S+HLCLPGSRFFPSKYNREIKSLKMEVERLLM+IIQSRKD VEIGRS+ YGNDLLGMLL 
Sbjct: 242 SKHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDCVEIGRSSSYGNDLLGMLLN 301

Query: 303 EMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVKQV 362
           EMQ+KR +GFSLNLQLIMDECKTFFFAGHETTALLLTWT+MLLASNP+WQEKVRAEVKQV
Sbjct: 302 EMQKKRGNGFSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAEVKQV 361

Query: 363 CNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVL 422
           CNGGTPSV+HL KLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDL IPKGLSIWIPVL
Sbjct: 362 CNGGTPSVDHLPKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLHIPKGLSIWIPVL 421

Query: 423 AIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIILALFVS 482
           AIHHSEELWGKDANEFNPERFASKSF+ GRFIPFA GPRNCIGQ+FAMMEAKIILA+ +S
Sbjct: 422 AIHHSEELWGKDANEFNPERFASKSFTPGRFIPFATGPRNCIGQSFAMMEAKIILAMLIS 481

Query: 483 NFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLN 516
            FSFTIS  YRHAPV+VLTI+PK+GVQVC+TP++
Sbjct: 482 RFSFTISPNYRHAPVIVLTIKPKYGVQVCMTPID 515

BLAST of Sgr021106 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.6e-216
Identity = 363/514 (70.62%), Postives = 437/514 (85.02%), Query Frame = 0

Query: 6   LLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDVAALVA 65
           +L +LLVI V+++ ++ YDTISCYWLTPRRI+K ME+QG+ GP+PRPL GNIL+++A+V+
Sbjct: 5   ILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVS 64

Query: 66  KSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELLSKNSS 125
           +S + D   I HDIVGRLLPHY+AW+KQYGKRF+ WNGT+PRLCL ETELIKELL K++ 
Sbjct: 65  QSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNG 124

Query: 126 VCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECTKNMIQ 185
           V G+SWLQ+QGTK+FIG+GLLMANG+DW+HQRH+ APAF G++LK YA +MVECT  +++
Sbjct: 125 VSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVE 184

Query: 186 SLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQASRHL 245
            L+ E+  G  E EI E M +LTADIISRT+F SS++KGK++F+ LT+LQR CAQA+RHL
Sbjct: 185 RLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHL 244

Query: 246 CLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLLKEMQ- 305
           C PGSRF PSKYNREIKSLK EVERLL++IIQSR+D  E+GRS+ +G+DLLG+LL EM  
Sbjct: 245 CFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDI 304

Query: 306 RKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVKQVC-N 365
            K  +  + NLQLIMDECKTFFFAGHETTALLLTWT MLLA NPTWQEKVR EV++V   
Sbjct: 305 DKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGR 364

Query: 366 GGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAI 425
            G PSV+ LSKLT L+ VINESLRLYPPAT+LPRMAFED+KLGDL IPKGLSIWIPVLAI
Sbjct: 365 NGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAI 424

Query: 426 HHSEELWGKDANEFNPERFASKSFSAGR-FIPFAAGPRNCIGQAFAMMEAKIILALFVSN 485
           HHSEELWGKDAN+FNPERF  + F++GR FIPFAAGPRNCIGQ FA+MEAKIILA  +S 
Sbjct: 425 HHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISK 484

Query: 486 FSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
           F+FTIS  YRHAP+VVLTI+PK+GVQV L PL S
Sbjct: 485 FNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Sgr021106 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 3.5e-211
Identity = 360/517 (69.63%), Postives = 433/517 (83.75%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           M   ++L  +LVI+++ + ++ YD+I CY+LTPRRI+K ME+QG+ GP+PR L GNI+D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
           + +++ S +ND S I H+IV RLLPHY++W+KQYGKRF+ WNGTEPRLCL ETE+IKELL
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           +K++ V GKSWLQ+QGTK FIG+GLLMANGE W+HQRH+ APAF  D+LK YA +MVECT
Sbjct: 121 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 180

Query: 181 KNMIQSLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQ 240
           K M + L+ E+     E EI E M++LTADIISRTEF SS DKGK++F LLT+LQRLCAQ
Sbjct: 181 KMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLL 300
           A+RHLC PGSRF PSKYNREIKSLK EVERLLM+II SRKD VEIGRS+ YG+DLLG+LL
Sbjct: 241 ATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLL 300

Query: 301 KEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVKQ 360
            +M   +    +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NPTWQ+ VR EV+Q
Sbjct: 301 NQMDSNKN---NLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQ 360

Query: 361 VC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIP 420
           VC   G PSVE LS LT LN VINESLRLYPPAT+LPRMAFEDIKLGDL IPKGLSIWIP
Sbjct: 361 VCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIP 420

Query: 421 VLAIHHSEELWGKDANEFNPERFASKSFSAGR-FIPFAAGPRNCIGQAFAMMEAKIILAL 480
           VLAIHHS ELWG+DANEFNPERF ++SF++ R F+PFAAGPRNCIGQ FAMMEAKIILA+
Sbjct: 421 VLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAM 480

Query: 481 FVSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLN 516
            VS FSF IS+ YRHAP+VVLTI+PK+GVQ+ L PL+
Sbjct: 481 LVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Sgr021106 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 3.0e-101
Identity = 208/525 (39.62%), Postives = 310/525 (59.05%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           M  VI L  +++IL+  L +I    +   W  P  + +R++ QG+ GP  R   GN+ ++
Sbjct: 1   MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
             +  +S  + +    +DI  R+LPHY  W  QYG+ F+YWNGTEPR+C+++ EL K +L
Sbjct: 61  KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTML 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           S       KS  + +  K    KGL+   G DW   R I+ PAF  D+LK     MV+CT
Sbjct: 121 SNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCT 180

Query: 181 KNMIQSLQTELNSGRTEF-----EIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQ 240
             M++  + E     TE      E+ E  ++LTADII+ + F SSY +G ++F     L+
Sbjct: 181 LKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELK 240

Query: 241 RLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDL 300
           R    +   + +PG+++ P+  N  +  L+ +++  + +II SR     +   + YG+DL
Sbjct: 241 RCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDL 300

Query: 301 LGMLLKEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVR 360
           LG+LLK    + ++   ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQEK+R
Sbjct: 301 LGILLKAYNTEGKER-KMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLR 360

Query: 361 AEVKQVC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGL 420
            E+ + C    TP  E  SKL L+NMVI ESLRLY P + L R A  +IKLGDLEIPKG 
Sbjct: 361 EEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGT 420

Query: 421 SIWIPVLAIHHSEELWGKDANEFNPERFASKSFSAGR----FIPFAAGPRNCIGQAFAMM 480
           ++ IP+L +H  + LWG DA++FNP RFA+    A       + F+ GPR CIGQ F M+
Sbjct: 421 TVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFVMI 480

Query: 481 EAKIILALFVSNFSF-TISDTYRHAPVVVLTIQPKHGVQVCLTPL 515
           EAK +L + +  F F ++ D Y+H PV  +TIQP++G+ V L PL
Sbjct: 481 EAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Sgr021106 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 6.7e-101
Identity = 215/522 (41.19%), Postives = 302/522 (57.85%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           ++ + LL   LV+LV  + KI Y         P  + +R +KQG+ GP+ R L GN+ ++
Sbjct: 59  LSTINLLAIALVLLV--VPKI-YGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREI 118

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
             +  ++    +    +DIV R+LPH   W  QYG+ F+YW GT+PRLC+++ EL K++L
Sbjct: 119 RKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQIL 178

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           S       KS  + +  K   G GL+  NG DW   R I+ PAF  DKLK     MV+CT
Sbjct: 179 SNKFVFFSKSKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCT 238

Query: 181 KNMIQSLQTELNSGRTE--FEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLC 240
             M    + + N   TE    I+   K+LTADII+   F SSY +G ++F     LQ+ C
Sbjct: 239 FRMFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCC 298

Query: 241 AQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGM 300
           A A   L  PG ++ P+  N +I  L M+V   + +II +R        S  YGNDLLG+
Sbjct: 299 AAALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGI 358

Query: 301 LLKEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEV 360
           +L       E    +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQEK+R EV
Sbjct: 359 MLTAAS-SNESEKKMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEV 418

Query: 361 KQVC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIW 420
              C     P  E  SKL L+N V  ESLRLY P   L R+A ED+KLG+LEIPKG +I 
Sbjct: 419 FNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTII 478

Query: 421 IPVLAIHHSEELWGKDANEFNPERFASKSFSAGR----FIPFAAGPRNCIGQAFAMMEAK 480
           +P+  +H  + +WG DA++FNP RFA+    A       + F+ GPR CIGQ FA+MEAK
Sbjct: 479 LPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAK 538

Query: 481 IILALFVSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLN 516
            +LA+ +  F   +S  Y+HAP   LT+QP++ + V L P++
Sbjct: 539 TVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of Sgr021106 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.5e-97
Identity = 190/516 (36.82%), Postives = 299/516 (57.95%), Query Frame = 0

Query: 11  LVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDVAALVAKSTAN 70
           +++L   L+ +    +S  W  PR+I +   KQG+RGP     IGN+ ++  ++ K++++
Sbjct: 13  VLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSH 72

Query: 71  DMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELLSKNSSVCGKS 130
            M    H+I+ R+L  Y  W K YG  F+ W G   RL +A+ +LI+E+ SK+     + 
Sbjct: 73  PMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEFY--EK 132

Query: 131 WLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECTKNMIQSLQTE 190
                  K   G GLL   GE W H R I++P F  + LK     +++   +M+     +
Sbjct: 133 NEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDK 192

Query: 191 LN-SGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQASRHLCLPG 250
           L+ +G  E ++ E+ + LT D+ISRT F SSY+ G+ +F L      LCA+A + + +PG
Sbjct: 193 LSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPG 252

Query: 251 SRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGR-STC---YGNDLLGMLLKEMQR 310
            RFFP++ N +   L  E+ + L+++I+ R+     G    C      DLLG++++    
Sbjct: 253 YRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAK-- 312

Query: 311 KREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVKQVCNG- 370
                 ++ +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K R EV +VC   
Sbjct: 313 ------NVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSR 372

Query: 371 GTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIH 430
             P+ +H+ KL  L+M++NESLRLYPP     R A  D+KLG  +IP G  + IP++A+H
Sbjct: 373 DVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVH 432

Query: 431 HSEELWGKDANEFNPERFASKSFSAGR----FIPFAAGPRNCIGQAFAMMEAKIILALFV 490
           H + +WG D NEFNP RFA     A +    FIPF  G R CIGQ  A+++AK+ LA+ +
Sbjct: 433 HDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMI 492

Query: 491 SNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
             F+F ++ TY+HAP V++ + P+HG  +    L +
Sbjct: 493 QRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTN 517

BLAST of Sgr021106 vs. ExPASy TrEMBL
Match: A0A1S3CGC0 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 5.8e-265
Identity = 451/520 (86.73%), Postives = 484/520 (93.08%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           MA +I LTTLLVI +SSLAKI YDTISCYWLTPRRIRK+MEKQG+RGP PRPL+GNILDV
Sbjct: 1   MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
           AAL+AKSTA DMS IDH+IV RLLPHY  WTKQYGKRF+YWNG EPRLCLAETELIKE+L
Sbjct: 61  AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           SKNS+VCG+SWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAP+FMGDKLKSYAG MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 KNMIQSLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQ 240
             +IQSL+TEL+SGRTEFEIA++MKQLTADIISRTEFD++ DKG QIFHLLTLLQ LCAQ
Sbjct: 181 DKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLM+IIQSRKDGVEIGRSTCYG+DLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 KEMQRKRED---GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAE 360
            EMQRK++D   GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLA+NP WQ+KVR E
Sbjct: 301 NEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VKQVC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSI 420
           V QVC NG TPSVEHLSK TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIIL 480
           WIPVLAIHHSEELWGKD NEF PERFASK FS GRFIPFAAGPRNC+GQ FA+MEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIIL 480

Query: 481 ALFVSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
           A+ +S FSFTISD+YRHAPV VLTIQPK+GVQV LTP+NS
Sbjct: 481 AMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Sgr021106 vs. ExPASy TrEMBL
Match: A0A0A0L6Y6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.4e-263
Identity = 449/520 (86.35%), Postives = 482/520 (92.69%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           MA +I+LTTLLVI +SSLAKI YDTISCYWLTPRRIRK+MEKQG+RGP PRPL+GNILDV
Sbjct: 1   MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
           AAL+ KSTA DMS IDH+IV RLLPHY  WTKQYGKRF+YWNG EPRLCLAETELIKE+L
Sbjct: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           SKNS+VCG+SWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAP+FMGDKLKSYAG MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 KNMIQSLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQ 240
             +IQSL+TE +SGRTEFEIA++MKQLTADIISRTEFD + DKG QIFHLLTLLQ LCAQ
Sbjct: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLM+IIQSRKDGVEIGRSTCYG+DLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 KEMQRKRED---GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAE 360
            EMQ++RED   GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLA+NP WQ+KVR E
Sbjct: 301 NEMQKRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VKQVC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSI 420
           V QVC NG TPSVEHLSK TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIIL 480
           WIPVLAIHHSEELWGKD NEF PERFASK FS GRFIPFAAGPRNCIGQ FA+MEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL 480

Query: 481 ALFVSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
           A+ +S FSF+ISD+YRHAPV VLTIQPK+GVQV LTP+NS
Sbjct: 481 AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Sgr021106 vs. ExPASy TrEMBL
Match: A0A6J1J2Y1 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.4e-257
Identity = 438/517 (84.72%), Postives = 471/517 (91.10%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           MA +I LTTL VI +SSLAKI YDT S Y LTPRRIRK ME+QG+RGP+PR LIGNILDV
Sbjct: 1   MAFIIFLTTLFVIFISSLAKISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
             LVAKSTANDMS IDHDIV RLLPHY  WTKQYGKRF+YWNGTEPRLCLAETELIKELL
Sbjct: 61  VGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQYGKRFIYWNGTEPRLCLAETELIKELL 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
            K SSVCG+SWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAG+MVECT
Sbjct: 121 GKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGFMVECT 180

Query: 181 KNMIQSLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQ 240
             MIQS++TEL+SGRTEFEIA++MKQLTADIISRTEFD+  DKG QIFHLLTLLQRLCAQ
Sbjct: 181 NKMIQSMETELDSGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLL 300
           ASRHLCLPGSRF PSKYNREIKSLK EVERLLM+II+SRKDGVEIGRS+CYG+DLLGMLL
Sbjct: 241 ASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLL 300

Query: 301 KEM-QRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVK 360
            EM QRK     +LNLQLI+DECKTFFFAGHETTALLLTWTIMLLA+NP WQ  VRAEV 
Sbjct: 301 NEMQQRKNSTTTTLNLQLIIDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVN 360

Query: 361 QVCNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIP 420
           QVCN  TPS++HLSKLTLLNMVINESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIP
Sbjct: 361 QVCNARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIP 420

Query: 421 VLAIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIILALF 480
           VLAIHHSEELWGKDAN+FNP RFA+K+F  GRFIPFA GPRNCIGQ FA+MEAKIILA+F
Sbjct: 421 VLAIHHSEELWGKDANQFNPNRFAAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMF 480

Query: 481 VSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
           +S FSFTISDTYRHAPVVVLTIQPKHGVQVCLTP +S
Sbjct: 481 LSKFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPFSS 517

BLAST of Sgr021106 vs. ExPASy TrEMBL
Match: A0A314Y2T9 (Cytokinin hydroxylase OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_04126 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.1e-255
Identity = 429/514 (83.46%), Postives = 478/514 (93.00%), Query Frame = 0

Query: 3   AVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDVAA 62
           A +LLTTLL+IL+S L ++ YDT+SCY+LTPRRI+K MEKQG+RGP+PRPL GNILD+A+
Sbjct: 2   AAVLLTTLLIILISLLIRVAYDTLSCYFLTPRRIKKIMEKQGVRGPKPRPLNGNILDMAS 61

Query: 63  LVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELLSK 122
           LVAKST++DM  I HDIVGRLLPHY+ W+KQYGKRF+YW G EPR+CLAETELIKELLSK
Sbjct: 62  LVAKSTSHDMQTISHDIVGRLLPHYVLWSKQYGKRFIYWTGIEPRMCLAETELIKELLSK 121

Query: 123 NSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECTKN 182
            S++ GKSWLQ+QG+KHFIG+GLLMANGEDWYHQRHIVAPAFMGD+LKSYAGYMVECTK 
Sbjct: 122 YSTISGKSWLQQQGSKHFIGRGLLMANGEDWYHQRHIVAPAFMGDRLKSYAGYMVECTKK 181

Query: 183 MIQSLQTEL-NSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQA 242
           M+QSLQ E+  SG  EFEI E+M +LTADIISRTEFDSSY+KGKQIF LLT+LQ LC+QA
Sbjct: 182 MLQSLQNEIETSGGREFEIGEYMTRLTADIISRTEFDSSYEKGKQIFRLLTVLQHLCSQA 241

Query: 243 SRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLLK 302
           S+HLCLPGSRFFPSKYNREIKSLKMEVERLLM+IIQSRKD VEIGRS+ YGNDLLGMLL 
Sbjct: 242 SKHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDCVEIGRSSSYGNDLLGMLLN 301

Query: 303 EMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVKQV 362
           EMQ+KR +GFSLNLQLIMDECKTFFFAGHETTALLLTWT+MLLASNP+WQEKVRAEVKQV
Sbjct: 302 EMQKKRGNGFSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAEVKQV 361

Query: 363 CNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVL 422
           CNGGTPSV+HL KLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDL IPKGLSIWIPVL
Sbjct: 362 CNGGTPSVDHLPKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLHIPKGLSIWIPVL 421

Query: 423 AIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIILALFVS 482
           AIHHSEELWGKDANEFNPERFASKSF+ GRFIPFA GPRNCIGQ+FAMMEAKIILA+ +S
Sbjct: 422 AIHHSEELWGKDANEFNPERFASKSFTPGRFIPFATGPRNCIGQSFAMMEAKIILAMLIS 481

Query: 483 NFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLN 516
            FSFTIS  YRHAPV+VLTI+PK+GVQVC+TP++
Sbjct: 482 RFSFTISPNYRHAPVIVLTIKPKYGVQVCMTPID 515

BLAST of Sgr021106 vs. ExPASy TrEMBL
Match: A0A5N5GF57 (Cytokinin hydroxylase-like OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_035999 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.4e-255
Identity = 428/514 (83.27%), Postives = 478/514 (93.00%), Query Frame = 0

Query: 3   AVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDVAA 62
           A +LLTTLLVI +  L ++ YDT+SCY+LTPRRI++ MEKQG+RGP+PRPL GNILD+A 
Sbjct: 2   AAVLLTTLLVIFLGLLLRVAYDTLSCYFLTPRRIKRTMEKQGVRGPKPRPLNGNILDMAT 61

Query: 63  LVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELLSK 122
           LVAKST++DM  IDHDIVGRLLPHY+AW+KQYGKRF+YWNG EPR+CL+ETELIKELLSK
Sbjct: 62  LVAKSTSHDMHTIDHDIVGRLLPHYVAWSKQYGKRFIYWNGIEPRMCLSETELIKELLSK 121

Query: 123 NSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECTKN 182
            S++ GKSWLQ+QG+KHFIG+GLLMANGEDWYHQRHIVAPAF GD+LKSYAGYMVECTK 
Sbjct: 122 YSTISGKSWLQQQGSKHFIGRGLLMANGEDWYHQRHIVAPAFTGDRLKSYAGYMVECTKE 181

Query: 183 MIQSLQTEL-NSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQA 242
           M+QSL+ E+  SG+ EFEI E+M +LTADIISRTEFDSSY+KGKQIF LLT+LQ LCAQA
Sbjct: 182 MLQSLKNEIETSGKREFEIGEYMTRLTADIISRTEFDSSYEKGKQIFRLLTVLQHLCAQA 241

Query: 243 SRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLLK 302
           SRHLCLPGSRFFPSKYNREIKSLKMEVERLLM+IIQSR+D VEIGRS+ YGNDLLGMLL 
Sbjct: 242 SRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLN 301

Query: 303 EMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVKQV 362
           EMQ+KR +GFSLNLQLIMDECKTFFFAGHETTALLLTWT+MLLASNP+WQEKVRAEVKQV
Sbjct: 302 EMQKKRGNGFSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAEVKQV 361

Query: 363 CNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVL 422
           CNGGTPSV+HLSKLTLLN VINESLRLYPPATVLPRMAFEDIKLGDL IPKGLSIWIPVL
Sbjct: 362 CNGGTPSVDHLSKLTLLNRVINESLRLYPPATVLPRMAFEDIKLGDLHIPKGLSIWIPVL 421

Query: 423 AIHHSEELWGKDANEFNPERFASKSFSAGRFIPFAAGPRNCIGQAFAMMEAKIILALFVS 482
           AIHHSEELWGKDANEFNPERFASKSF+ GRFIPFA GPRNCIGQ+FAMMEAKIILA+ +S
Sbjct: 422 AIHHSEELWGKDANEFNPERFASKSFTPGRFIPFATGPRNCIGQSFAMMEAKIILAMLIS 481

Query: 483 NFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLN 516
            FSFT+S  YRHAPV+VLTI+PK+GVQVCLTP++
Sbjct: 482 QFSFTVSPNYRHAPVIVLTIKPKYGVQVCLTPID 515

BLAST of Sgr021106 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 753.4 bits (1944), Expect = 1.2e-217
Identity = 363/514 (70.62%), Postives = 437/514 (85.02%), Query Frame = 0

Query: 6   LLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDVAALVA 65
           +L +LLVI V+++ ++ YDTISCYWLTPRRI+K ME+QG+ GP+PRPL GNIL+++A+V+
Sbjct: 5   ILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVS 64

Query: 66  KSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELLSKNSS 125
           +S + D   I HDIVGRLLPHY+AW+KQYGKRF+ WNGT+PRLCL ETELIKELL K++ 
Sbjct: 65  QSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNG 124

Query: 126 VCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECTKNMIQ 185
           V G+SWLQ+QGTK+FIG+GLLMANG+DW+HQRH+ APAF G++LK YA +MVECT  +++
Sbjct: 125 VSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVE 184

Query: 186 SLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQASRHL 245
            L+ E+  G  E EI E M +LTADIISRT+F SS++KGK++F+ LT+LQR CAQA+RHL
Sbjct: 185 RLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHL 244

Query: 246 CLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLLKEMQ- 305
           C PGSRF PSKYNREIKSLK EVERLL++IIQSR+D  E+GRS+ +G+DLLG+LL EM  
Sbjct: 245 CFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDI 304

Query: 306 RKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVKQVC-N 365
            K  +  + NLQLIMDECKTFFFAGHETTALLLTWT MLLA NPTWQEKVR EV++V   
Sbjct: 305 DKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGR 364

Query: 366 GGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAI 425
            G PSV+ LSKLT L+ VINESLRLYPPAT+LPRMAFED+KLGDL IPKGLSIWIPVLAI
Sbjct: 365 NGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAI 424

Query: 426 HHSEELWGKDANEFNPERFASKSFSAGR-FIPFAAGPRNCIGQAFAMMEAKIILALFVSN 485
           HHSEELWGKDAN+FNPERF  + F++GR FIPFAAGPRNCIGQ FA+MEAKIILA  +S 
Sbjct: 425 HHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISK 484

Query: 486 FSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
           F+FTIS  YRHAP+VVLTI+PK+GVQV L PL S
Sbjct: 485 FNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Sgr021106 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 735.7 bits (1898), Expect = 2.5e-212
Identity = 360/517 (69.63%), Postives = 433/517 (83.75%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           M   ++L  +LVI+++ + ++ YD+I CY+LTPRRI+K ME+QG+ GP+PR L GNI+D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
           + +++ S +ND S I H+IV RLLPHY++W+KQYGKRF+ WNGTEPRLCL ETE+IKELL
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           +K++ V GKSWLQ+QGTK FIG+GLLMANGE W+HQRH+ APAF  D+LK YA +MVECT
Sbjct: 121 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 180

Query: 181 KNMIQSLQTELNSGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQ 240
           K M + L+ E+     E EI E M++LTADIISRTEF SS DKGK++F LLT+LQRLCAQ
Sbjct: 181 KMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGMLL 300
           A+RHLC PGSRF PSKYNREIKSLK EVERLLM+II SRKD VEIGRS+ YG+DLLG+LL
Sbjct: 241 ATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLL 300

Query: 301 KEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVKQ 360
            +M   +    +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NPTWQ+ VR EV+Q
Sbjct: 301 NQMDSNKN---NLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQ 360

Query: 361 VC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIP 420
           VC   G PSVE LS LT LN VINESLRLYPPAT+LPRMAFEDIKLGDL IPKGLSIWIP
Sbjct: 361 VCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIP 420

Query: 421 VLAIHHSEELWGKDANEFNPERFASKSFSAGR-FIPFAAGPRNCIGQAFAMMEAKIILAL 480
           VLAIHHS ELWG+DANEFNPERF ++SF++ R F+PFAAGPRNCIGQ FAMMEAKIILA+
Sbjct: 421 VLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAM 480

Query: 481 FVSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLN 516
            VS FSF IS+ YRHAP+VVLTI+PK+GVQ+ L PL+
Sbjct: 481 LVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Sgr021106 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 370.5 bits (950), Expect = 2.1e-102
Identity = 208/525 (39.62%), Postives = 310/525 (59.05%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           M  VI L  +++IL+  L +I    +   W  P  + +R++ QG+ GP  R   GN+ ++
Sbjct: 1   MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
             +  +S  + +    +DI  R+LPHY  W  QYG+ F+YWNGTEPR+C+++ EL K +L
Sbjct: 61  KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTML 120

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           S       KS  + +  K    KGL+   G DW   R I+ PAF  D+LK     MV+CT
Sbjct: 121 SNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCT 180

Query: 181 KNMIQSLQTELNSGRTEF-----EIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQ 240
             M++  + E     TE      E+ E  ++LTADII+ + F SSY +G ++F     L+
Sbjct: 181 LKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELK 240

Query: 241 RLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDL 300
           R    +   + +PG+++ P+  N  +  L+ +++  + +II SR     +   + YG+DL
Sbjct: 241 RCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDL 300

Query: 301 LGMLLKEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVR 360
           LG+LLK    + ++   ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQEK+R
Sbjct: 301 LGILLKAYNTEGKER-KMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLR 360

Query: 361 AEVKQVC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGL 420
            E+ + C    TP  E  SKL L+NMVI ESLRLY P + L R A  +IKLGDLEIPKG 
Sbjct: 361 EEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGT 420

Query: 421 SIWIPVLAIHHSEELWGKDANEFNPERFASKSFSAGR----FIPFAAGPRNCIGQAFAMM 480
           ++ IP+L +H  + LWG DA++FNP RFA+    A       + F+ GPR CIGQ F M+
Sbjct: 421 TVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFVMI 480

Query: 481 EAKIILALFVSNFSF-TISDTYRHAPVVVLTIQPKHGVQVCLTPL 515
           EAK +L + +  F F ++ D Y+H PV  +TIQP++G+ V L PL
Sbjct: 481 EAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Sgr021106 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 369.4 bits (947), Expect = 4.7e-102
Identity = 215/522 (41.19%), Postives = 302/522 (57.85%), Query Frame = 0

Query: 1   MAAVILLTTLLVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDV 60
           ++ + LL   LV+LV  + KI Y         P  + +R +KQG+ GP+ R L GN+ ++
Sbjct: 59  LSTINLLAIALVLLV--VPKI-YGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREI 118

Query: 61  AALVAKSTANDMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELL 120
             +  ++    +    +DIV R+LPH   W  QYG+ F+YW GT+PRLC+++ EL K++L
Sbjct: 119 RKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQIL 178

Query: 121 SKNSSVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECT 180
           S       KS  + +  K   G GL+  NG DW   R I+ PAF  DKLK     MV+CT
Sbjct: 179 SNKFVFFSKSKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCT 238

Query: 181 KNMIQSLQTELNSGRTE--FEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLC 240
             M    + + N   TE    I+   K+LTADII+   F SSY +G ++F     LQ+ C
Sbjct: 239 FRMFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCC 298

Query: 241 AQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGRSTCYGNDLLGM 300
           A A   L  PG ++ P+  N +I  L M+V   + +II +R        S  YGNDLLG+
Sbjct: 299 AAALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGI 358

Query: 301 LLKEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEV 360
           +L       E    +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQEK+R EV
Sbjct: 359 MLTAAS-SNESEKKMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEV 418

Query: 361 KQVC-NGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIW 420
              C     P  E  SKL L+N V  ESLRLY P   L R+A ED+KLG+LEIPKG +I 
Sbjct: 419 FNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTII 478

Query: 421 IPVLAIHHSEELWGKDANEFNPERFASKSFSAGR----FIPFAAGPRNCIGQAFAMMEAK 480
           +P+  +H  + +WG DA++FNP RFA+    A       + F+ GPR CIGQ FA+MEAK
Sbjct: 479 LPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAK 538

Query: 481 IILALFVSNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLN 516
            +LA+ +  F   +S  Y+HAP   LT+QP++ + V L P++
Sbjct: 539 TVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of Sgr021106 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 358.2 bits (918), Expect = 1.1e-98
Identity = 190/516 (36.82%), Postives = 299/516 (57.95%), Query Frame = 0

Query: 11  LVILVSSLAKIGYDTISCYWLTPRRIRKRMEKQGLRGPEPRPLIGNILDVAALVAKSTAN 70
           +++L   L+ +    +S  W  PR+I +   KQG+RGP     IGN+ ++  ++ K++++
Sbjct: 13  VLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSH 72

Query: 71  DMSGIDHDIVGRLLPHYIAWTKQYGKRFMYWNGTEPRLCLAETELIKELLSKNSSVCGKS 130
            M    H+I+ R+L  Y  W K YG  F+ W G   RL +A+ +LI+E+ SK+     + 
Sbjct: 73  PMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEFY--EK 132

Query: 131 WLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGYMVECTKNMIQSLQTE 190
                  K   G GLL   GE W H R I++P F  + LK     +++   +M+     +
Sbjct: 133 NEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDK 192

Query: 191 LN-SGRTEFEIAEFMKQLTADIISRTEFDSSYDKGKQIFHLLTLLQRLCAQASRHLCLPG 250
           L+ +G  E ++ E+ + LT D+ISRT F SSY+ G+ +F L      LCA+A + + +PG
Sbjct: 193 LSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPG 252

Query: 251 SRFFPSKYNREIKSLKMEVERLLMQIIQSRKDGVEIGR-STC---YGNDLLGMLLKEMQR 310
            RFFP++ N +   L  E+ + L+++I+ R+     G    C      DLLG++++    
Sbjct: 253 YRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAK-- 312

Query: 311 KREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLASNPTWQEKVRAEVKQVCNG- 370
                 ++ +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K R EV +VC   
Sbjct: 313 ------NVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSR 372

Query: 371 GTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIH 430
             P+ +H+ KL  L+M++NESLRLYPP     R A  D+KLG  +IP G  + IP++A+H
Sbjct: 373 DVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVH 432

Query: 431 HSEELWGKDANEFNPERFASKSFSAGR----FIPFAAGPRNCIGQAFAMMEAKIILALFV 490
           H + +WG D NEFNP RFA     A +    FIPF  G R CIGQ  A+++AK+ LA+ +
Sbjct: 433 HDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMI 492

Query: 491 SNFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPLNS 517
             F+F ++ TY+HAP V++ + P+HG  +    L +
Sbjct: 493 QRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTN 517

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905687.14.2e-27089.00cytokinin hydroxylase-like [Benincasa hispida][more]
XP_008461587.11.2e-26486.73PREDICTED: cytokinin hydroxylase-like [Cucumis melo][more]
XP_004145660.12.9e-26386.35cytokinin hydroxylase [Cucumis sativus] >KGN57750.1 hypothetical protein Csa_009... [more]
XP_022983651.19.2e-25784.72cytokinin hydroxylase-like [Cucurbita maxima][more]
PQQ00573.12.3e-25583.46cytokinin hydroxylase [Prunus yedoensis var. nudiflora][more]
Match NameE-valueIdentityDescription
Q9FF181.6e-21670.62Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1[more]
Q9ZW953.5e-21169.63Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1[more]
Q9ASR33.0e-10139.62Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1[more]
F4IK456.7e-10141.19Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1[more]
O487861.5e-9736.82Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CGC05.8e-26586.73cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1[more]
A0A0A0L6Y61.4e-26386.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1[more]
A0A6J1J2Y14.4e-25784.72cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1[more]
A0A314Y2T91.1e-25583.46Cytokinin hydroxylase OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_04126... [more]
A0A5N5GF571.4e-25583.27Cytokinin hydroxylase-like OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D... [more]
Match NameE-valueIdentityDescription
AT5G38450.11.2e-21770.62cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT1G67110.12.5e-21269.63cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT2G46960.22.1e-10239.62cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
AT2G46950.14.7e-10241.19cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT2G26710.11.1e-9836.82Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 261..281
NoneNo IPR availablePANTHERPTHR24282:SF186CYTOCHROME P450 FAMILY PROTEINcoord: 4..510
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 4..510
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 462..485
score: 29.73
coord: 91..110
score: 26.05
coord: 317..334
score: 29.73
coord: 337..363
score: 29.25
coord: 452..462
score: 52.03
coord: 420..444
score: 25.72
coord: 207..225
score: 20.27
IPR001128Cytochrome P450PRINTSPR00385P450coord: 380..391
score: 46.75
coord: 328..345
score: 40.88
coord: 462..473
score: 43.74
coord: 453..462
score: 57.09
IPR001128Cytochrome P450PFAMPF00067p450coord: 91..489
e-value: 1.1E-89
score: 301.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 29..515
e-value: 3.5E-117
score: 393.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 47..513

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr021106.1Sgr021106.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen