Sgr020768 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr020768
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Trypsin inhibitor 1-like
Location	tig00153554: 906803 .. 907078 (+)
RNA-Seq Expression	Sgr020768
Synteny	Sgr020768

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGCAGCAAGATTGTTGCGGTGGTGCTGGTAGTGGCGATGATGCTGCTGGCCACGTCCGACTCTGCCATGGACATCGACACAGTGGAACTCATCTCCGATGATGGTCGAGCTCAAACTGATCAAGGCATCAATGGCTTCCCAAGAACATTACTGAGAGGCGGGGCGTTTTACGAGGAAAAAAGAGTCTGTCCCAGAATCCTCATGCCATGCAAAAGCGACTCCGACTGCTTGGTCCAGTGTATTTGCCTAGCGAATGGGTATTGTGGTTAA

mRNA sequence

ATGGAGAGCAGCAAGATTGTTGCGGTGGTGCTGGTAGTGGCGATGATGCTGCTGGCCACGTCCGACTCTGCCATGGACATCGACACAGTGGAACTCATCTCCGATGATGGTCGAGCTCAAACTGATCAAGGCATCAATGGCTTCCCAAGAACATTACTGAGAGGCGGGGCGTTTTACGAGGAAAAAAGAGTCTGTCCCAGAATCCTCATGCCATGCAAAAGCGACTCCGACTGCTTGGTCCAGTGTATTTGCCTAGCGAATGGGTATTGTGGTTAA

Coding sequence (CDS)

ATGGAGAGCAGCAAGATTGTTGCGGTGGTGCTGGTAGTGGCGATGATGCTGCTGGCCACGTCCGACTCTGCCATGGACATCGACACAGTGGAACTCATCTCCGATGATGGTCGAGCTCAAACTGATCAAGGCATCAATGGCTTCCCAAGAACATTACTGAGAGGCGGGGCGTTTTACGAGGAAAAAAGAGTCTGTCCCAGAATCCTCATGCCATGCAAAAGCGACTCCGACTGCTTGGTCCAGTGTATTTGCCTAGCGAATGGGTATTGTGGTTAA

Protein sequence

MESSKIVAVVLVVAMMLLATSDSAMDIDTVELISDDGRAQTDQGINGFPRTLLRGGAFYEEKRVCPRILMPCKSDSDCLVQCICLANGYCG

Homology

[Close]

BLAST of Sgr020768 vs. NCBI nr
Match: AIZ03439.1 (TIPRE3 precursor, partial [Momordica anigosantha])

HSP 1 Score: 80.5 bits (197), Expect = 8.3e-12
Identity = 44/81 (54.32%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 11 LVVAMMLLATSDSAMDIDTVELISDDGRAQTDQGINGFPRTLLRGGAFYEEKRVCPRILM 70
          ++VA ML+ATS    D DT++LIS+D RAQT Q ING       GG + EE+R CPRIL 
Sbjct: 3  VLVATMLVATSADFNDGDTIDLISND-RAQTGQDING-------GGVYSEEQRACPRILK 62

Query: 71 PCKSDSDCLVQCICLANGYCG 92
           C+ DSDC   C+C  NGYCG
Sbjct: 63 RCRRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of Sgr020768 vs. NCBI nr
Match: AIZ03440.1 (TIPRE4 precursor, partial [Momordica anigosantha])

HSP 1 Score: 80.5 bits (197), Expect = 8.3e-12
Identity = 44/81 (54.32%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 11 LVVAMMLLATSDSAMDIDTVELISDDGRAQTDQGINGFPRTLLRGGAFYEEKRVCPRILM 70
          ++VA ML+ATS    D DT++LIS+D RAQT Q ING       GG + EE+R CPRIL 
Sbjct: 3  VLVATMLVATSADFNDGDTIDLISND-RAQTGQDING-------GGVYSEEQRACPRILK 62

Query: 71 PCKSDSDCLVQCICLANGYCG 92
           C+ DSDC   C+C  NGYCG
Sbjct: 63 RCRRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of Sgr020768 vs. NCBI nr
Match: KAG6576919.1 (hypothetical protein SDJN03_24493, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 80.1 bits (196), Expect = 1.1e-11
Identity = 47/94 (50.00%), Postives = 64/94 (68.09%), Query Frame = 0


Query: 1   MESSKIVAVVLVVAMMLLATSDS---AMDIDTVELISDDGRAQTDQGINGFPRTLLRGGA 60
           M S KIVAV L +A++L+AT  S   A+D D+++L+  DGR       +G PR +++ G 
Sbjct: 54  MGSMKIVAVAL-IAILLVATLSSAAYAIDDDSMDLVF-DGRDIN----HGVPRKIMKWGV 113

Query: 61  FYEEKRVCPRILMPCKSDSDCLVQCICLANGYCG 92
              E+RVCP+ILM CK DSDCL +CICL +GYCG
Sbjct: 114 LNHEERVCPKILMECKKDSDCLAECICLEHGYCG 141

[Close]

BLAST of Sgr020768 vs. NCBI nr
Match: KAG7014944.1 (hypothetical protein SDJN02_22575, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 80.1 bits (196), Expect = 1.1e-11
Identity = 47/94 (50.00%), Postives = 64/94 (68.09%), Query Frame = 0


Query: 1   MESSKIVAVVLVVAMMLLATSDS---AMDIDTVELISDDGRAQTDQGINGFPRTLLRGGA 60
           M S KIVAV L +A++L+AT  S   A+D D+++L+  DGR       +G PR +++ G 
Sbjct: 54  MGSMKIVAVAL-IAILLVATLSSAAYAIDDDSMDLVF-DGRDIN----HGVPRKIMKWGV 113

Query: 61  FYEEKRVCPRILMPCKSDSDCLVQCICLANGYCG 92
              E+RVCP+ILM CK DSDCL +CICL +GYCG
Sbjct: 114 LNHEERVCPKILMECKKDSDCLAECICLEHGYCG 141

[Close]

BLAST of Sgr020768 vs. NCBI nr
Match: KAA0044339.1 (trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibitor 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 79.7 bits (195), Expect = 1.4e-11
Identity = 48/97 (49.48%), Postives = 63/97 (64.95%), Query Frame = 0


Query: 1  MESSKIVAVVLV-VAMMLLATSDSAM----DIDTVELISDDGRAQTDQGINGFPR-TLLR 60
          M+S KIV V +V + +ML A S SA     D D+  L+S DGR       NG PR  +++
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVS-DGRDMN----NGCPRKIMIK 60

Query: 61 GGAFYEEKRVCPRILMPCKSDSDCLVQCICLANGYCG 92
          GG F EE+R+CP+ILM CK DSDCL+ C+CL  G+CG
Sbjct: 61 GGVFKEEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of Sgr020768 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.8e-10
Identity = 31/75 (41.33%), Postives = 46/75 (61.33%), Query Frame = 0


Query: 17 LLATSDSAMDIDTVELISDDGRAQTDQGINGFPRTLLRGGAFYEEKRVCPRILMPCKSDS 76
          + + ++S+  ++ +ELISD G        N  PR ++ G        +CPRILMPCK+D 
Sbjct: 1  MASVAESSGVVEVIELISDGG--------NDLPRKIMSG----RHGGICPRILMPCKTDD 60

Query: 77 DCLVQCICLANGYCG 92
          DC++ C CL+NGYCG
Sbjct: 61 DCMLDCRCLSNGYCG 63

[Close]

BLAST of Sgr020768 vs. ExPASy Swiss-Prot
Match: P07853 (Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 1.2e-08
Identity = 23/31 (74.19%), Postives = 27/31 (87.10%), Query Frame = 0


Query: 61 EKRVCPRILMPCKSDSDCLVQCICLANGYCG 92
          E+RVCPRILM CK DSDCL +C+CL +GYCG
Sbjct: 2  EERVCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of Sgr020768 vs. ExPASy Swiss-Prot
Match: P10293 (Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 2.0e-08
Identity = 23/31 (74.19%), Postives = 27/31 (87.10%), Query Frame = 0


Query: 61 EKRVCPRILMPCKSDSDCLVQCICLANGYCG 92
          E+RVCP+ILM CK DSDCL +CICL +GYCG
Sbjct: 2  EERVCPKILMECKKDSDCLAECICLEHGYCG 32

[Close]

BLAST of Sgr020768 vs. ExPASy Swiss-Prot
Match: P35628 (Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 3.4e-08
Identity = 22/28 (78.57%), Postives = 25/28 (89.29%), Query Frame = 0


Query: 64 VCPRILMPCKSDSDCLVQCICLANGYCG 92
          +CPRILMPC SDSDCL +CICL NG+CG
Sbjct: 1  ICPRILMPCSSDSDCLAECICLENGFCG 28

[Close]

BLAST of Sgr020768 vs. ExPASy Swiss-Prot
Match: P01074 (Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 7.6e-08
Identity = 22/29 (75.86%), Postives = 25/29 (86.21%), Query Frame = 0


Query: 63 RVCPRILMPCKSDSDCLVQCICLANGYCG 92
          RVCPRILM CK DSDCL +C+CL +GYCG
Sbjct: 1  RVCPRILMECKKDSDCLAECVCLEHGYCG 29

[Close]

BLAST of Sgr020768 vs. ExPASy TrEMBL
Match: A0A0A7HF94 (TIPRE3 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE3 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.0e-12
Identity = 44/81 (54.32%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 11 LVVAMMLLATSDSAMDIDTVELISDDGRAQTDQGINGFPRTLLRGGAFYEEKRVCPRILM 70
          ++VA ML+ATS    D DT++LIS+D RAQT Q ING       GG + EE+R CPRIL 
Sbjct: 3  VLVATMLVATSADFNDGDTIDLISND-RAQTGQDING-------GGVYSEEQRACPRILK 62

Query: 71 PCKSDSDCLVQCICLANGYCG 92
           C+ DSDC   C+C  NGYCG
Sbjct: 63 RCRRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of Sgr020768 vs. ExPASy TrEMBL
Match: A0A0A7HIT2 (TIPRE4 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE4 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.0e-12
Identity = 44/81 (54.32%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 11 LVVAMMLLATSDSAMDIDTVELISDDGRAQTDQGINGFPRTLLRGGAFYEEKRVCPRILM 70
          ++VA ML+ATS    D DT++LIS+D RAQT Q ING       GG + EE+R CPRIL 
Sbjct: 3  VLVATMLVATSADFNDGDTIDLISND-RAQTGQDING-------GGVYSEEQRACPRILK 62

Query: 71 PCKSDSDCLVQCICLANGYCG 92
           C+ DSDC   C+C  NGYCG
Sbjct: 63 RCRRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of Sgr020768 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.9e-12
Identity = 48/97 (49.48%), Postives = 63/97 (64.95%), Query Frame = 0


Query: 1  MESSKIVAVVLV-VAMMLLATSDSAM----DIDTVELISDDGRAQTDQGINGFPR-TLLR 60
          M+S KIV V +V + +ML A S SA     D D+  L+S DGR       NG PR  +++
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVS-DGRDMN----NGCPRKIMIK 60

Query: 61 GGAFYEEKRVCPRILMPCKSDSDCLVQCICLANGYCG 92
          GG F EE+R+CP+ILM CK DSDCL+ C+CL  G+CG
Sbjct: 61 GGVFKEEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of Sgr020768 vs. ExPASy TrEMBL
Match: A0A0A0KV23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 9.0e-12
Identity = 51/99 (51.52%), Postives = 67/99 (67.68%), Query Frame = 0


Query: 1  MESSKIVAVVLVVAMML-LATSDSAM----DIDTVELISDDGRAQTDQGI-NGFPR-TLL 60
          MES KIV V +V  +++ +A S SA     D D ++L+S DGRAQ DQ + NG PR  ++
Sbjct: 1  MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVS-DGRAQADQDMNNGCPRKMMI 60

Query: 61 RGGAFYEEKRVCPRILMPCKSDSDCLVQCICLAN-GYCG 92
          +GG F EE+ +CPRILM CK DSDCL  C+CL +  YCG
Sbjct: 61 KGGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEHIEYCG 98

[Close]

BLAST of Sgr020768 vs. ExPASy TrEMBL
Match: A0A0A7HIA9 (TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 7.6e-11
Identity = 42/80 (52.50%), Postives = 52/80 (65.00%), Query Frame = 0


Query: 12 VVAMMLLATSDSAMDIDTVELISDDGRAQTDQGINGFPRTLLRGGAFYEEKRVCPRILMP 71
          +VAMML+ATS  +   DT+ LIS +GRAQT Q IN        GG + EE+R CP+IL  
Sbjct: 4  LVAMMLVATSADSNGGDTIHLIS-NGRAQTGQDINS-------GGVYSEEQRACPKILKR 63

Query: 72 CKSDSDCLVQCICLANGYCG 92
          C+ DSDC   C+C  NGYCG
Sbjct: 64 CRRDSDCPGACVCQDNGYCG 75

The following BLAST results are available for this feature:

BLAST of Sgr020768 vs. NCBI nr


Match Name	E-value	Identity	Description
AIZ03439.1	8.3e-12	54.32	TIPRE3 precursor, partial [Momordica anigosantha]	[more]
AIZ03440.1	8.3e-12	54.32	TIPRE4 precursor, partial [Momordica anigosantha]	[more]
KAG6576919.1	1.1e-11	50.00	hypothetical protein SDJN03_24493, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014944.1	1.1e-11	50.00	hypothetical protein SDJN02_22575, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAA0044339.1	1.4e-11	49.48	trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibito...	[more]

BLAST of Sgr020768 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P34950	2.8e-10	41.33	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P07853	1.2e-08	74.19	Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2	[more]
P10293	2.0e-08	74.19	Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
P35628	3.4e-08	78.57	Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P01074	7.6e-08	75.86	Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]

BLAST of Sgr020768 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A7HF94	4.0e-12	54.32	TIPRE3 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE3 PE=3 SV=1	[more]
A0A0A7HIT2	4.0e-12	54.32	TIPRE4 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE4 PE=3 SV=1	[more]
A0A5D3E148	6.9e-12	49.48	Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A0A0KV23	9.0e-12	51.52	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1	[more]
A0A0A7HIA9	7.6e-11	52.50	TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1	[more]

BLAST of Sgr020768 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PRINTS	PR00293	SQUASHINHBTR	coord: 75..84 score: 71.5 coord: 65..74 score: 76.0
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 63..91 e-value: 3.6E-17 score: 62.1
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 65..84
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 65..91 e-value: 6.09393E-12 score: 52.7651
None	No IPR available	SMART	SM00286	PTI_1	coord: 63..91 e-value: 1.7E-13 score: 60.9
None	No IPR available	GENE3D	4.10.75.20		coord: 44..91 e-value: 6.9E-16 score: 59.8
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 60..91

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr020768.1	Sgr020768.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity