Sgr020673 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr020673
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionSpoU_methylase domain-containing protein
Locationtig00153552: 1060125 .. 1063157 (+)
RNA-Seq ExpressionSgr020673
SyntenySgr020673
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAATGCTCATGCATTTCCTGGTCGACAAATCTAAAACTCGGACCTTCCATTTCCAAGTCGAAAGTACCAATTGCCCAAGCAGAGGCAGTGGCCGCCGATATTGCTTCCACTGCCGGAAGAATTTCTCTACCTTCTCATGTGAAACCCATCACCAGCACCTCCAACCCCTTTGTTAAGCATTGTGTAAAACTCCGCTTCAGTTCTTCTTATCGCCACTTCCATGGCTCCGTGCTCGTCGTCGGCGCTACCCCAATAAGGTCGCCTTTTTATCCATAATTTTGTCCCAACTTTTCTGTGTTCTGTTCATAATCGTGTTCGTGGTTGAAAAATCAATTTCGTCTATGCAGGGTTTTTGCTTTTTAAATCTTTTCATCGATAAATTTCATTAGCTCTCGAGAAAGAAAAAGGGAAAGCAAAACCCTTAACTTCTGTTCAAATCTATATTAAATTGTCATATGGTTTAAGCAATTCTCTTACACAATTTATTAGGGCTTTGTCTTTTATACTATTTTTCGCTGCTTAATTAATTGGAATCTTAAATAGGTTTCGCGCACACAATTTTAACCTGGATAATGTTATGTGAATGCTGTTCTGATGGTCCGAAGTAAAGACTGAGTAATGTTTTATGAATTTCCCCTGAATTATCGTTTGATGTATAAGCAACCAAAAGTAATGTACACACTCATTTTTAATTGTTCCATCGTTTGTTGGCAAATGCTGTTGAAGAACTAAGGTCTTGGAATCAAGTCCAGTGTAATAGCAAGCTTGTGAATGAATATTTTGGAAGCACCCTTAAGCATCCTCCAGAGTTTTATTTACTTTGACTGTTCTCTACTGAACTATGACTTATTTTCTGTTTATTGAACTTTGAGTACTCGAAATTGGTTAACAAAATTATAAATTGTTAGAGTTATGTAGAATTGGTGCTTTCCTACTATAATATCAGCTTAAAATCCCTCTTGTGAAGTCACATTTTGTTGTTATGGTTTTTTTGTTTAATCTACAGGTAACTCTGTCCATTGTCTGCAAGGAAGACTATCCTATTCCTCGTGTGGATGCTTGCACATATTGCAGTTCATTTCTAGTTGGAATTGTTGAAAGTTATTGTGAACTATGGTCGTCTTATCTACTCAAATCATTTAAATTCAATCTTTTTTGGAGCATAACGGAATTTATCTAATGGTCTTCCCATGAGAAAATTTGCTATCGTACTTTTTTACATTGGATTAGCCCTCCCTCCCCCTGGATCTAAGGAGTTCTGTCTTGTATCAGTTCATTTGTCTGAACTCTGAAGTGGCTATTCTTTGAGGTAAAATGTTTTGCGTGACTTCATATGGTTTGTATGCTCTTACATCTAAATTGTTTACAGGGAAATTTGCAAGTTTCAAGAGTCTCTGCATGGGACAACTAGTGCATTGGAGTGCCTACTTCTGCTAGATGAAGTTGAGGTCCCTGAAGGACTAGTTGATATATGTGGTAGTATTGTGCGGGTGAGCCCGACCGTGATGAAAAAGCTTTCTGGCATGCAATCAACAGAATCTATTGATGCCATTGCCCTCATGAGATTACCTGCCAGTTTTTGTAGTGTAGATCACAATGAAAAAGAAGCACATTTTAAAAGATGGTTTCCATCTCCACATCGAATACTAGTCCTTGAGGGAATCCAGGTAATTCTTTCATTTGGAGTTAAATGAAGTCTTGTAGTTTGGTGCATCTAATTGTCCTGTCTCTAAGTTGTTACAAAATAGGTTGCATTTCCCCCCATTTCATTCTATCATTACTGCTAAGATGACTTTTCTACCTCGTGTTAAGCTGTCTCTCAAGGCCACTGTGTATGGTATGTCCCTGTGCACATTGACATGGTAATAGTTTTTTTGTATTCACTTGTAAAGAGCAAATGAGCTACAGTCCACTTAATCTATCTTTAGTGCCCTGATGATAACCTTTATGCTTAGCATTTGACATTATAAAGTATTACAGATAGCTTTGTTGATTGAGTATCCATATATTAACATTAAATTTACCAGTTCATCTTAGTTTATTGCAGGACCCGGGCAATCTTGGTACATTAATCCGGTCTGCTTTGGCCTTCAAATGGGTAAGAATAAATTCCACAAACTACTATTATCCTTCAGTCTTTGACCTCTTTTTTCCCGCAATATCCTCTTCATGCTGTGGGGAGCGGGGGTATCACACAATGCACTTATTGGCATTCTATTTCGACTAAGAATACTCACTGGAGGAAATTTGTCTCTCATGGGTTAATGTTTTTTTTGGAAAAATATTCTATCGATTCAGATCTTCTCATTCAGTTTGAATAACCTTTATTTTAACCCCAAATTTCAATGAATGCTGATGGAATACGTCACCTTCATAGTTTGACATTGCCTATAGGGTTAGATATGACATCCCATGTTGAAGTAATACACCTCACATTCCTTTTATGCATCGTTTCTCTTTATTGCTAGAGAAACCGATCGAGAAATAATAATCCTGGCAATTGTTTTTCAATTTTATTTGTCTTCTTAAAGTAATTTACTTTTATATAAAAGATGAATTGAAAAACTTTTAAAACTCTATTTTCTTACAAGCTTTTACATTCTCTGGTATATATTTGGTTATGCCAAACAGAATGGTGTTTTTCTGCTTCCTGGGTGTTGCGATCCATTCAACGAGAAGGCCCTCCGAGCCAGTCGAGGAGCATGTTTCCAGCTTCCAATAGTCTCTGGCACTTGGAATCATCTTGAGACCCTAAAAGATGAATTCCAAATGAAAATGCTGGCTGGTCATCCCGAAAGTAATGAACCGAAGCCAGTTTCGTTCCTTACGCAACAGTTTGCAAATTCTTTGAAAGATTTTCCGTTGTGCTTGGTTTTGGGTTGTGAAGGGAGTGGTCTGTCTGAGAAATCTTGGCAGGATTGTGAACTTTTGAGCATTCCAATGGCGGGTGGTTTCGAGTCCCTCAATGTTTCGGTTGCAGGAGGAATTTTCTTGTACATGCTACAGACTGAAAACCAAAGACATATTTGA

mRNA sequence

ATGCAATGCTCATGCATTTCCTGGTCGACAAATCTAAAACTCGGACCTTCCATTTCCAAGTCGAAAGTACCAATTGCCCAAGCAGAGGCAGTGGCCGCCGATATTGCTTCCACTGCCGGAAGAATTTCTCTACCTTCTCATGTGAAACCCATCACCAGCACCTCCAACCCCTTTGTTAAGCATTGTGTAAAACTCCGCTTCAGTTCTTCTTATCGCCACTTCCATGGCTCCGTGCTCGTAACTCTGTCCATTGTCTGCAAGGAAGACTATCCTATTCCTCGTGTGGATGCTTGCACATATTGCAGTTCATTTCTAGTTGGAATTGTTGAAAGTTATTGTGAACTATGGGAAATTTGCAAGTTTCAAGAGTCTCTGCATGGGACAACTAGTGCATTGGAGTGCCTACTTCTGCTAGATGAAGTTGAGGTCCCTGAAGGACTAGTTGATATATGTGGTAGTATTGTGCGGGTGAGCCCGACCGTGATGAAAAAGCTTTCTGGCATGCAATCAACAGAATCTATTGATGCCATTGCCCTCATGAGATTACCTGCCAGTTTTTGTAGTGTAGATCACAATGAAAAAGAAGCACATTTTAAAAGATGGTTTCCATCTCCACATCGAATACTAGTCCTTGAGGGAATCCAGGACCCGGGCAATCTTGGTACATTAATCCGGTCTGCTTTGGCCTTCAAATGGAATGGTGTTTTTCTGCTTCCTGGGTGTTGCGATCCATTCAACGAGAAGGCCCTCCGAGCCAGTCGAGGAGCATGTTTCCAGCTTCCAATAGTCTCTGGCACTTGGAATCATCTTGAGACCCTAAAAGATGAATTCCAAATGAAAATGCTGGCTGGTCATCCCGAAAGTAATGAACCGAAGCCAGTTTCGTTCCTTACGCAACAGTTTGCAAATTCTTTGAAAGATTTTCCGTTGTGCTTGGTTTTGGGTTGTGAAGGGAGTGGTCTGTCTGAGAAATCTTGGCAGGATTGTGAACTTTTGAGCATTCCAATGGCGGGTGGTTTCGAGTCCCTCAATGTTTCGGTTGCAGGAGGAATTTTCTTGTACATGCTACAGACTGAAAACCAAAGACATATTTGA

Coding sequence (CDS)

ATGCAATGCTCATGCATTTCCTGGTCGACAAATCTAAAACTCGGACCTTCCATTTCCAAGTCGAAAGTACCAATTGCCCAAGCAGAGGCAGTGGCCGCCGATATTGCTTCCACTGCCGGAAGAATTTCTCTACCTTCTCATGTGAAACCCATCACCAGCACCTCCAACCCCTTTGTTAAGCATTGTGTAAAACTCCGCTTCAGTTCTTCTTATCGCCACTTCCATGGCTCCGTGCTCGTAACTCTGTCCATTGTCTGCAAGGAAGACTATCCTATTCCTCGTGTGGATGCTTGCACATATTGCAGTTCATTTCTAGTTGGAATTGTTGAAAGTTATTGTGAACTATGGGAAATTTGCAAGTTTCAAGAGTCTCTGCATGGGACAACTAGTGCATTGGAGTGCCTACTTCTGCTAGATGAAGTTGAGGTCCCTGAAGGACTAGTTGATATATGTGGTAGTATTGTGCGGGTGAGCCCGACCGTGATGAAAAAGCTTTCTGGCATGCAATCAACAGAATCTATTGATGCCATTGCCCTCATGAGATTACCTGCCAGTTTTTGTAGTGTAGATCACAATGAAAAAGAAGCACATTTTAAAAGATGGTTTCCATCTCCACATCGAATACTAGTCCTTGAGGGAATCCAGGACCCGGGCAATCTTGGTACATTAATCCGGTCTGCTTTGGCCTTCAAATGGAATGGTGTTTTTCTGCTTCCTGGGTGTTGCGATCCATTCAACGAGAAGGCCCTCCGAGCCAGTCGAGGAGCATGTTTCCAGCTTCCAATAGTCTCTGGCACTTGGAATCATCTTGAGACCCTAAAAGATGAATTCCAAATGAAAATGCTGGCTGGTCATCCCGAAAGTAATGAACCGAAGCCAGTTTCGTTCCTTACGCAACAGTTTGCAAATTCTTTGAAAGATTTTCCGTTGTGCTTGGTTTTGGGTTGTGAAGGGAGTGGTCTGTCTGAGAAATCTTGGCAGGATTGTGAACTTTTGAGCATTCCAATGGCGGGTGGTTTCGAGTCCCTCAATGTTTCGGTTGCAGGAGGAATTTTCTTGTACATGCTACAGACTGAAAACCAAAGACATATTTGA

Protein sequence

MQCSCISWSTNLKLGPSISKSKVPIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFVKHCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEICKFQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIALMRLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQQFANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTENQRHI
Homology
BLAST of Sgr020673 vs. NCBI nr
Match: XP_022136373.1 (uncharacterized protein LOC111008099 [Momordica charantia])

HSP 1 Score: 576.6 bits (1485), Expect = 1.5e-160
Identity = 295/364 (81.04%), Postives = 306/364 (84.07%), Query Frame = 0

Query: 1   MQCSCISWSTNLKLGPSISKSKVPIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFVK 60
           MQCSCI WSTNLKLGPSISKSKV IA AE +       AGRISLPSHVK ITSTSNPFVK
Sbjct: 1   MQCSCIFWSTNLKLGPSISKSKVSIAGAETI-----DIAGRISLPSHVKAITSTSNPFVK 60

Query: 61  HCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEICK 120
           HCVKLRFSSSYRHFHGSVLV                         VG       + EIC+
Sbjct: 61  HCVKLRFSSSYRHFHGSVLV-------------------------VGAT----PIREICR 120

Query: 121 FQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIALM 180
           FQE+LHGTTS LECLLLLDEV+VPEGLVD+   IVRVSPTVMKKLSGMQSTESIDAIALM
Sbjct: 121 FQETLHGTTSTLECLLLLDEVDVPEGLVDVSVRIVRVSPTVMKKLSGMQSTESIDAIALM 180

Query: 181 RLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLPG 240
           R+PASF SV HNEKEA+F+RWFPS HRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLPG
Sbjct: 181 RIPASFSSVYHNEKEANFRRWFPSLHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLPG 240

Query: 241 CCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQQ 300
           CCDPFNEKALRASRGA FQLPIVSGTWNHLE LK+EFQMKMLAGHPESNEPKPVSFL+QQ
Sbjct: 241 CCDPFNEKALRASRGASFQLPIVSGTWNHLEALKNEFQMKMLAGHPESNEPKPVSFLSQQ 300

Query: 301 FANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTEN 360
           FANSLKDFPLCLVLGCEGSGLSEKSWQ CELLSIPMAGGFESLNVSVAGGIFLYMLQ EN
Sbjct: 301 FANSLKDFPLCLVLGCEGSGLSEKSWQVCELLSIPMAGGFESLNVSVAGGIFLYMLQNEN 330

Query: 361 QRHI 365
             HI
Sbjct: 361 HTHI 330

BLAST of Sgr020673 vs. NCBI nr
Match: XP_038904874.1 (uncharacterized tRNA/rRNA methyltransferase slr1673 [Benincasa hispida])

HSP 1 Score: 540.8 bits (1392), Expect = 9.0e-150
Identity = 279/365 (76.44%), Postives = 296/365 (81.10%), Query Frame = 0

Query: 1   MQCSCISWSTNLKLGPSISKSKV-PIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFV 60
           MQCSC+SWS+NLKLGPS SKSK+  I QAE +       A R+SLPSHVK ITSTSNPFV
Sbjct: 1   MQCSCVSWSSNLKLGPSFSKSKLSSINQAETI-----DFAARVSLPSHVKAITSTSNPFV 60

Query: 61  KHCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEIC 120
           KHCVKLRFSSSYRH+HG+VLV                         VG       + EIC
Sbjct: 61  KHCVKLRFSSSYRHYHGTVLV-------------------------VGAT----PIREIC 120

Query: 121 KFQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIAL 180
           KFQESLHG T  LECLLLLD+VEVPEGLVD    IVRVSPTVM+KLSGMQSTESIDAIAL
Sbjct: 121 KFQESLHGITCTLECLLLLDDVEVPEGLVDKSVRIVRVSPTVMRKLSGMQSTESIDAIAL 180

Query: 181 MRLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLP 240
           MR+PASFCSVD NEK+  FKR FPSPHRILVLE IQDPGNLGTLIRSALAFKWNGVFLLP
Sbjct: 181 MRIPASFCSVDGNEKDIDFKRCFPSPHRILVLERIQDPGNLGTLIRSALAFKWNGVFLLP 240

Query: 241 GCCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQ 300
           GCCDP+NEKALRASRGA FQLPIVSGTWNHLE LK++FQMKMLAGHPESNEPK VS L+Q
Sbjct: 241 GCCDPYNEKALRASRGASFQLPIVSGTWNHLEVLKNKFQMKMLAGHPESNEPKRVSVLSQ 300

Query: 301 QFANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTE 360
           QFANSLK+FPLCLVLGCEGSGLS KSW  CELLSIPMAGGFESLNVSVAGGIFLYMLQ E
Sbjct: 301 QFANSLKEFPLCLVLGCEGSGLSGKSWHVCELLSIPMAGGFESLNVSVAGGIFLYMLQAE 331

Query: 361 NQRHI 365
           N  HI
Sbjct: 361 NNSHI 331

BLAST of Sgr020673 vs. NCBI nr
Match: XP_011650605.1 (uncharacterized protein LOC101218901 isoform X2 [Cucumis sativus] >KAE8650203.1 hypothetical protein Csa_010011 [Cucumis sativus])

HSP 1 Score: 534.6 bits (1376), Expect = 6.4e-148
Identity = 275/360 (76.39%), Postives = 289/360 (80.28%), Query Frame = 0

Query: 1   MQCSCISWSTNLKLGPSISKSKV-PIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFV 60
           MQCSC+SWS+N KL PS  KSK+  I  AEA+        GRI LPSHVK ITS SNPFV
Sbjct: 1   MQCSCLSWSSNPKLAPSFPKSKLSSINIAEAI-----DFTGRIPLPSHVKAITSISNPFV 60

Query: 61  KHCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEIC 120
           KHCVKLRFSSSYRHFHGSV+V                         VG       + EIC
Sbjct: 61  KHCVKLRFSSSYRHFHGSVVV-------------------------VGAT----PIREIC 120

Query: 121 KFQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIAL 180
           KFQESLHG T  LECLLLLDEVEVPEGLV++   IVRVS  VMKKLSG+QSTESIDAIAL
Sbjct: 121 KFQESLHGVTCTLECLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIAL 180

Query: 181 MRLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLP 240
           MR+P SFCSVD NEKE  FKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNG+FLLP
Sbjct: 181 MRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGIFLLP 240

Query: 241 GCCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQ 300
           GCCDPFNEKALRASRGA FQLPIVSGTWNHLE  K EF+MKMLAGHPESNEPKPVS L+Q
Sbjct: 241 GCCDPFNEKALRASRGASFQLPIVSGTWNHLEVFKKEFEMKMLAGHPESNEPKPVSVLSQ 300

Query: 301 QFANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTE 360
           QF+NSLKDFPLCLVLGCEGSGLSEKSWQ CELLSIPMAGGFESLNVSVAGGIFLYMLQ E
Sbjct: 301 QFSNSLKDFPLCLVLGCEGSGLSEKSWQVCELLSIPMAGGFESLNVSVAGGIFLYMLQAE 326

BLAST of Sgr020673 vs. NCBI nr
Match: XP_031739071.1 (uncharacterized protein LOC101218901 isoform X1 [Cucumis sativus])

HSP 1 Score: 534.3 bits (1375), Expect = 8.4e-148
Identity = 275/360 (76.39%), Postives = 289/360 (80.28%), Query Frame = 0

Query: 1   MQCSCISWSTNLKLGPSISKSKV-PIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFV 60
           MQCSC+SWS+N KL PS  KSK+  I  AEA+        GRI LPSHVK ITS SNPFV
Sbjct: 1   MQCSCLSWSSNPKLAPSFPKSKLSSINIAEAI-----DFTGRIPLPSHVKAITSISNPFV 60

Query: 61  KHCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEIC 120
           KHCVKLRFSSSYRHFHGSV+V                  T  S +            EIC
Sbjct: 61  KHCVKLRFSSSYRHFHGSVVVV---------------GATPISLYR-----------EIC 120

Query: 121 KFQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIAL 180
           KFQESLHG T  LECLLLLDEVEVPEGLV++   IVRVS  VMKKLSG+QSTESIDAIAL
Sbjct: 121 KFQESLHGVTCTLECLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIAL 180

Query: 181 MRLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLP 240
           MR+P SFCSVD NEKE  FKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNG+FLLP
Sbjct: 181 MRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGIFLLP 240

Query: 241 GCCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQ 300
           GCCDPFNEKALRASRGA FQLPIVSGTWNHLE  K EF+MKMLAGHPESNEPKPVS L+Q
Sbjct: 241 GCCDPFNEKALRASRGASFQLPIVSGTWNHLEVFKKEFEMKMLAGHPESNEPKPVSVLSQ 300

Query: 301 QFANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTE 360
           QF+NSLKDFPLCLVLGCEGSGLSEKSWQ CELLSIPMAGGFESLNVSVAGGIFLYMLQ E
Sbjct: 301 QFSNSLKDFPLCLVLGCEGSGLSEKSWQVCELLSIPMAGGFESLNVSVAGGIFLYMLQAE 329

BLAST of Sgr020673 vs. NCBI nr
Match: XP_008450171.1 (PREDICTED: uncharacterized tRNA/rRNA methyltransferase YsgA isoform X1 [Cucumis melo] >KAA0060378.1 putative tRNA/rRNA methyltransferase YsgA isoform X1 [Cucumis melo var. makuwa] >TYK22118.1 putative tRNA/rRNA methyltransferase YsgA isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 531.2 bits (1367), Expect = 7.1e-147
Identity = 276/360 (76.67%), Postives = 289/360 (80.28%), Query Frame = 0

Query: 1   MQCSCISWSTNLKLGPSISKSKV-PIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFV 60
           MQCSC+SWS+N KLGPS  KSK+  I QAEA+        GRISLPSHVK I+S SNPFV
Sbjct: 1   MQCSCLSWSSNPKLGPSFHKSKLSSINQAEAI-----DFTGRISLPSHVKAISSISNPFV 60

Query: 61  KHCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEIC 120
           KHCVKLRFSSSYRHFHGSV+V                         VG       + EIC
Sbjct: 61  KHCVKLRFSSSYRHFHGSVVV-------------------------VGAT----PIREIC 120

Query: 121 KFQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIAL 180
           KFQESLHG T  LE LLLLDEVEVPEGLV++   IVRVS  VMKKLSG+QSTESIDAIAL
Sbjct: 121 KFQESLHGITCTLEYLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIAL 180

Query: 181 MRLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLP 240
           MR+P SFCSVD NEK   FKRWFPSPHRILVLE IQDPGNLGTLIRSALAFKWNGVFLLP
Sbjct: 181 MRIPPSFCSVDGNEKVVDFKRWFPSPHRILVLERIQDPGNLGTLIRSALAFKWNGVFLLP 240

Query: 241 GCCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQ 300
           GCCDPFNEKALRASRGA FQLPIVSGTWNHLE  K EF+MKMLAGHPESNEPKPVS L+Q
Sbjct: 241 GCCDPFNEKALRASRGASFQLPIVSGTWNHLEVFKKEFKMKMLAGHPESNEPKPVSVLSQ 300

Query: 301 QFANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTE 360
           QFANSLKDFPLCLVLGCEGSGLSEKSWQ CELLSIPMAGGFESLNVSVAGGIFLYMLQ E
Sbjct: 301 QFANSLKDFPLCLVLGCEGSGLSEKSWQVCELLSIPMAGGFESLNVSVAGGIFLYMLQAE 326

BLAST of Sgr020673 vs. ExPASy Swiss-Prot
Match: P94538 (Uncharacterized tRNA/rRNA methyltransferase YsgA OS=Bacillus subtilis (strain 168) OX=224308 GN=ysgA PE=3 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 1.4e-17
Identity = 60/196 (30.61%), Postives = 99/196 (50.51%), Query Frame = 0

Query: 168 MQSTESIDAIALMRLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSA 227
           M S ++  A+     P    +V H  +E        +  ++L+++ +QDPGNLGT+IR+A
Sbjct: 71  MLSEDAFSAVTETETPQQIAAVCHMPEEK-----LATARKVLLIDAVQDPGNLGTMIRTA 130

Query: 228 LAFKWNGVFLLPGCCDPFNEKALRASRGACFQLPIVSGTW-NHLETLKDE----FQMKML 287
            A   + V L  G  D FN K LR+++G+ F +P+V     ++++ LK E    +   + 
Sbjct: 131 DAAGLDAVVLGDGTADAFNGKTLRSAQGSHFHIPVVRRNLPSYVDELKAEGVKVYGTALQ 190

Query: 288 AGHPESNEPKPVSFLTQQFANSLKDFPLCLVLGCEGSGLSEKSWQDCEL-LSIPMAGGFE 347
            G P    P+  SF               L++G EG+G+     +  +L L +P+ G  E
Sbjct: 191 NGAPYQEIPQSESF--------------ALIVGNEGAGVDAALLEKTDLNLYVPLYGQAE 247

Query: 348 SLNVSVAGGIFLYMLQ 358
           SLNV+VA  I +Y L+
Sbjct: 251 SLNVAVAAAILVYHLR 247

BLAST of Sgr020673 vs. ExPASy Swiss-Prot
Match: P74261 (Uncharacterized tRNA/rRNA methyltransferase slr1673 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1673 PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.8e-15
Identity = 57/158 (36.08%), Postives = 83/158 (52.53%), Query Frame = 0

Query: 203 PSPH-RI-LVLEGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGACFQL 262
           P PH R+ LVLE +QDPGNLGT++R+A A    G++L   C DP + K LR+S G+   L
Sbjct: 115 PPPHARLGLVLERLQDPGNLGTILRTAAATGVEGIWLTADCVDPTSPKVLRSSAGSSLLL 174

Query: 263 PIVSGTWNHLETLKDEFQ---MKMLAGHPESNEPKPVSFLTQQFANSLKDFPLCLVLGCE 322
           P        L  L ++F    ++++A  P++         TQ         P  ++ G E
Sbjct: 175 P--QQQLQSLPPLLEKFHTQGLQLIATVPQA---------TQTLWEIDFQRPTIVIFGSE 234

Query: 323 GSGLSEKSWQ-DCELLSIPMAGGFESLNVSVAGGIFLY 355
           G GLS    +     ++IP A   ESLNV++A G+ LY
Sbjct: 235 GQGLSAPVLELTTHQVAIPQAPQVESLNVAIAVGVMLY 261

BLAST of Sgr020673 vs. ExPASy Swiss-Prot
Match: A0QAB6 (Uncharacterized tRNA/rRNA methyltransferase MAV_0574 OS=Mycobacterium avium (strain 104) OX=243243 GN=MAV_0574 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 2.6e-14
Identity = 56/154 (36.36%), Postives = 76/154 (49.35%), Query Frame = 0

Query: 205 PHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGACFQLPIVS 264
           P  ++ L+ I DP NLG ++RS  AF  +GV +           A R S GA  ++P+  
Sbjct: 159 PALLVALDNISDPRNLGAIVRSVAAFGGHGVLIPQRRSASVTAVAWRTSAGAAARIPVAR 218

Query: 265 GTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQQFANSLKDF----PLCLVLGCEGSG 324
            T N   TL+D            ++    V  L      +L D     PL +V+G EG G
Sbjct: 219 AT-NLTRTLQD-----------WADRGLRVIGLDAGGDTTLDDLDGSDPLVVVVGSEGKG 278

Query: 325 LSEKSWQDC-ELLSIPMAGGFESLNVSVAGGIFL 354
           LS    Q+C E++SIPMAG  ESLN SVA G+ L
Sbjct: 279 LSRLVRQNCDEVVSIPMAGSAESLNASVAAGVVL 300

BLAST of Sgr020673 vs. ExPASy Swiss-Prot
Match: Q9VW14 (rRNA methyltransferase 3, mitochondrial OS=Drosophila melanogaster OX=7227 GN=CG14100 PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 5.7e-14
Identity = 52/160 (32.50%), Postives = 86/160 (53.75%), Query Frame = 0

Query: 209 LVLEGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGACFQLPIVSG-TW 268
           +V + I++P NLG++IR+  A   + V +  GCCDP+  KALR   G  F++PI    TW
Sbjct: 238 VVCDNIREPNNLGSIIRTCAALPCSQVVVTHGCCDPWESKALRGGCGGQFRVPIRDDVTW 297

Query: 269 NHLE-TLKDEFQMKMLAGHPESNEPKPVSFLTQQFANSLKDFPL--CLVLGCEGSGLSEK 328
           + L  T+  E          E+N+ K  +  T  +A+ +K       L++G E  G+SE+
Sbjct: 298 DELALTIPPEAADDCHVFIAETNQRKRENNQTIDYAD-IKGLGAHNLLIIGGESHGVSEE 357

Query: 329 SWQDCELLS-------IPMAGGFESLNVSVAGGIFLYMLQ 358
           +++   L+        IP+A G +SLNV+ A  + L+ L+
Sbjct: 358 AYRFLNLVGGKGKCIYIPLAAGIDSLNVASALTLLLFELR 396

BLAST of Sgr020673 vs. ExPASy Swiss-Prot
Match: Q743W2 (Uncharacterized tRNA/rRNA methyltransferase MAP_0479 OS=Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10) OX=262316 GN=MAP_0479 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 5.7e-14
Identity = 53/150 (35.33%), Postives = 74/150 (49.33%), Query Frame = 0

Query: 205 PHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGACFQLPIVS 264
           P  ++ L+ I DP NLG ++RS  AF  +GV +           A R S GA  ++P+  
Sbjct: 159 PALLVALDNISDPRNLGAIVRSVAAFGGHGVLIPQRRSASVTAVAWRTSAGAAARIPVAR 218

Query: 265 GTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQQFANSLKDFPLCLVLGCEGSGLSEK 324
            T N   TL+D     +     ++     +  L           PL +V+G EG GLS  
Sbjct: 219 AT-NLTRTLQDWADRGLRVIGLDAGGDTMLDDLD-------GSDPLVVVVGSEGKGLSRL 278

Query: 325 SWQDC-ELLSIPMAGGFESLNVSVAGGIFL 354
             Q+C E++SIPMAG  ESLN SVA G+ L
Sbjct: 279 VRQNCDEVVSIPMAGSAESLNASVAAGVVL 300

BLAST of Sgr020673 vs. ExPASy TrEMBL
Match: A0A6J1C7F0 (uncharacterized protein LOC111008099 OS=Momordica charantia OX=3673 GN=LOC111008099 PE=4 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 7.2e-161
Identity = 295/364 (81.04%), Postives = 306/364 (84.07%), Query Frame = 0

Query: 1   MQCSCISWSTNLKLGPSISKSKVPIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFVK 60
           MQCSCI WSTNLKLGPSISKSKV IA AE +       AGRISLPSHVK ITSTSNPFVK
Sbjct: 1   MQCSCIFWSTNLKLGPSISKSKVSIAGAETI-----DIAGRISLPSHVKAITSTSNPFVK 60

Query: 61  HCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEICK 120
           HCVKLRFSSSYRHFHGSVLV                         VG       + EIC+
Sbjct: 61  HCVKLRFSSSYRHFHGSVLV-------------------------VGAT----PIREICR 120

Query: 121 FQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIALM 180
           FQE+LHGTTS LECLLLLDEV+VPEGLVD+   IVRVSPTVMKKLSGMQSTESIDAIALM
Sbjct: 121 FQETLHGTTSTLECLLLLDEVDVPEGLVDVSVRIVRVSPTVMKKLSGMQSTESIDAIALM 180

Query: 181 RLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLPG 240
           R+PASF SV HNEKEA+F+RWFPS HRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLPG
Sbjct: 181 RIPASFSSVYHNEKEANFRRWFPSLHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLPG 240

Query: 241 CCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQQ 300
           CCDPFNEKALRASRGA FQLPIVSGTWNHLE LK+EFQMKMLAGHPESNEPKPVSFL+QQ
Sbjct: 241 CCDPFNEKALRASRGASFQLPIVSGTWNHLEALKNEFQMKMLAGHPESNEPKPVSFLSQQ 300

Query: 301 FANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTEN 360
           FANSLKDFPLCLVLGCEGSGLSEKSWQ CELLSIPMAGGFESLNVSVAGGIFLYMLQ EN
Sbjct: 301 FANSLKDFPLCLVLGCEGSGLSEKSWQVCELLSIPMAGGFESLNVSVAGGIFLYMLQNEN 330

Query: 361 QRHI 365
             HI
Sbjct: 361 HTHI 330

BLAST of Sgr020673 vs. ExPASy TrEMBL
Match: A0A5D3DF85 (Putative tRNA/rRNA methyltransferase YsgA isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold318G001150 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.4e-147
Identity = 276/360 (76.67%), Postives = 289/360 (80.28%), Query Frame = 0

Query: 1   MQCSCISWSTNLKLGPSISKSKV-PIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFV 60
           MQCSC+SWS+N KLGPS  KSK+  I QAEA+        GRISLPSHVK I+S SNPFV
Sbjct: 1   MQCSCLSWSSNPKLGPSFHKSKLSSINQAEAI-----DFTGRISLPSHVKAISSISNPFV 60

Query: 61  KHCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEIC 120
           KHCVKLRFSSSYRHFHGSV+V                         VG       + EIC
Sbjct: 61  KHCVKLRFSSSYRHFHGSVVV-------------------------VGAT----PIREIC 120

Query: 121 KFQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIAL 180
           KFQESLHG T  LE LLLLDEVEVPEGLV++   IVRVS  VMKKLSG+QSTESIDAIAL
Sbjct: 121 KFQESLHGITCTLEYLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIAL 180

Query: 181 MRLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLP 240
           MR+P SFCSVD NEK   FKRWFPSPHRILVLE IQDPGNLGTLIRSALAFKWNGVFLLP
Sbjct: 181 MRIPPSFCSVDGNEKVVDFKRWFPSPHRILVLERIQDPGNLGTLIRSALAFKWNGVFLLP 240

Query: 241 GCCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQ 300
           GCCDPFNEKALRASRGA FQLPIVSGTWNHLE  K EF+MKMLAGHPESNEPKPVS L+Q
Sbjct: 241 GCCDPFNEKALRASRGASFQLPIVSGTWNHLEVFKKEFKMKMLAGHPESNEPKPVSVLSQ 300

Query: 301 QFANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTE 360
           QFANSLKDFPLCLVLGCEGSGLSEKSWQ CELLSIPMAGGFESLNVSVAGGIFLYMLQ E
Sbjct: 301 QFANSLKDFPLCLVLGCEGSGLSEKSWQVCELLSIPMAGGFESLNVSVAGGIFLYMLQAE 326

BLAST of Sgr020673 vs. ExPASy TrEMBL
Match: A0A1S3BNM2 (uncharacterized tRNA/rRNA methyltransferase YsgA isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491842 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.4e-147
Identity = 276/360 (76.67%), Postives = 289/360 (80.28%), Query Frame = 0

Query: 1   MQCSCISWSTNLKLGPSISKSKV-PIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFV 60
           MQCSC+SWS+N KLGPS  KSK+  I QAEA+        GRISLPSHVK I+S SNPFV
Sbjct: 1   MQCSCLSWSSNPKLGPSFHKSKLSSINQAEAI-----DFTGRISLPSHVKAISSISNPFV 60

Query: 61  KHCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEIC 120
           KHCVKLRFSSSYRHFHGSV+V                         VG       + EIC
Sbjct: 61  KHCVKLRFSSSYRHFHGSVVV-------------------------VGAT----PIREIC 120

Query: 121 KFQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIAL 180
           KFQESLHG T  LE LLLLDEVEVPEGLV++   IVRVS  VMKKLSG+QSTESIDAIAL
Sbjct: 121 KFQESLHGITCTLEYLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIAL 180

Query: 181 MRLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLP 240
           MR+P SFCSVD NEK   FKRWFPSPHRILVLE IQDPGNLGTLIRSALAFKWNGVFLLP
Sbjct: 181 MRIPPSFCSVDGNEKVVDFKRWFPSPHRILVLERIQDPGNLGTLIRSALAFKWNGVFLLP 240

Query: 241 GCCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQ 300
           GCCDPFNEKALRASRGA FQLPIVSGTWNHLE  K EF+MKMLAGHPESNEPKPVS L+Q
Sbjct: 241 GCCDPFNEKALRASRGASFQLPIVSGTWNHLEVFKKEFKMKMLAGHPESNEPKPVSVLSQ 300

Query: 301 QFANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTE 360
           QFANSLKDFPLCLVLGCEGSGLSEKSWQ CELLSIPMAGGFESLNVSVAGGIFLYMLQ E
Sbjct: 301 QFANSLKDFPLCLVLGCEGSGLSEKSWQVCELLSIPMAGGFESLNVSVAGGIFLYMLQAE 326

BLAST of Sgr020673 vs. ExPASy TrEMBL
Match: A0A6J1FNI0 (uncharacterized protein LOC111447061 OS=Cucurbita moschata OX=3662 GN=LOC111447061 PE=4 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.5e-145
Identity = 275/364 (75.55%), Postives = 289/364 (79.40%), Query Frame = 0

Query: 1   MQCSCISWSTNLKLGPSISKSKV-PIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFV 60
           MQCSCIS  +NLKLGPSISK K+  I + EA+       A RISLPSHVK ITSTSNPFV
Sbjct: 1   MQCSCISLPSNLKLGPSISKFKLSSIPKVEAI-----DLAARISLPSHVKAITSTSNPFV 60

Query: 61  KHCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEIC 120
           KHCVKLRF+SSYRHFHGSVLV                         VG       + EIC
Sbjct: 61  KHCVKLRFTSSYRHFHGSVLV-------------------------VGAT----PIREIC 120

Query: 121 KFQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIAL 180
           KFQESL G +  LECLLLLDEVE+PE LVD+   +VRVSPTVMKKLSGMQSTESIDAIAL
Sbjct: 121 KFQESLRGISCTLECLLLLDEVEIPEELVDVSVRVVRVSPTVMKKLSGMQSTESIDAIAL 180

Query: 181 MRLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLP 240
           MR+P SF S+D N K+AH KRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLP
Sbjct: 181 MRIPDSFYSLDGNLKQAHCKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLP 240

Query: 241 GCCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQ 300
           GCCDPFNEKALRASRGA FQLPIVSGTWNHLE LK EFQMKMLAGHPESNE KPVS L+Q
Sbjct: 241 GCCDPFNEKALRASRGASFQLPIVSGTWNHLEDLKTEFQMKMLAGHPESNELKPVSVLSQ 300

Query: 301 QFANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTE 360
           QFANS KD PLCLVLG EGSGLSEK WQ CELLSIPMAGGFESLNVSVAGGIFLYMLQ  
Sbjct: 301 QFANSSKDIPLCLVLGSEGSGLSEKCWQVCELLSIPMAGGFESLNVSVAGGIFLYMLQAV 330

Query: 361 NQRH 364
           N RH
Sbjct: 361 NDRH 330

BLAST of Sgr020673 vs. ExPASy TrEMBL
Match: A0A6J1JVY6 (uncharacterized protein LOC111488367 OS=Cucurbita maxima OX=3661 GN=LOC111488367 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.2e-144
Identity = 274/364 (75.27%), Postives = 289/364 (79.40%), Query Frame = 0

Query: 1   MQCSCISWSTNLKLGPSISKSKVPIAQAEAVAADIASTAGRISLPSHVKPITSTSNPFVK 60
           MQCSCIS  +NLKLGPSISK K+  +  +  A D+A+   RISLPSHVK ITSTSNPFVK
Sbjct: 1   MQCSCISLPSNLKLGPSISKFKLS-SNLKVEAIDLAA---RISLPSHVKAITSTSNPFVK 60

Query: 61  HCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVDACTYCSSFLVGIVESYCELWEICK 120
           HCVKLRFSSSYRHFHGSVLV                         VG       + EICK
Sbjct: 61  HCVKLRFSSSYRHFHGSVLV-------------------------VGAT----PIREICK 120

Query: 121 FQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVRVSPTVMKKLSGMQSTESIDAIALM 180
           FQESL G +  L CLLLLDEVEVPE LVD+   IVRVSPTVMKKLSGMQS ESIDAIALM
Sbjct: 121 FQESLRGISCTLACLLLLDEVEVPEELVDVSVRIVRVSPTVMKKLSGMQSIESIDAIALM 180

Query: 181 RLPASFCSVDHNEKEAHFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNGVFLLPG 240
           R+P SFCS+D N K+AH K WFPSPHRILVL+GIQDPGNLGTLIRSALAFKWNGVFLLPG
Sbjct: 181 RIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDGIQDPGNLGTLIRSALAFKWNGVFLLPG 240

Query: 241 CCDPFNEKALRASRGACFQLPIVSGTWNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQQ 300
           CCDPFNEKALRASRGA FQLPIVSGTWNHLE LK +FQMKMLAGHPESNE KPVS L+QQ
Sbjct: 241 CCDPFNEKALRASRGASFQLPIVSGTWNHLEALKTDFQMKMLAGHPESNELKPVSVLSQQ 300

Query: 301 FANSLKDFPLCLVLGCEGSGLSEKSWQDCELLSIPMAGGFESLNVSVAGGIFLYMLQTEN 360
           FANS KD  LCLVLG EGSGLSEKSWQ CELLSIPM GGFESLNVSVAGGIFLYMLQ EN
Sbjct: 301 FANSSKDISLCLVLGSEGSGLSEKSWQVCELLSIPMVGGFESLNVSVAGGIFLYMLQAEN 331

Query: 361 QRHI 365
            RHI
Sbjct: 361 DRHI 331

BLAST of Sgr020673 vs. TAIR 10
Match: AT4G38020.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 379.0 bits (972), Expect = 4.2e-105
Identity = 193/324 (59.57%), Postives = 237/324 (73.15%), Query Frame = 0

Query: 37  STAGRISLPSHVKPITSTSNPFVKHCVKLRFSSSYRHFHGSVLVTLSIVCKEDYPIPRVD 96
           S A R+SLPSHV  ITST+NPFVKHC+KLR SSSYRH HGSVLV  +I  +         
Sbjct: 55  SPANRVSLPSHVNSITSTTNPFVKHCLKLRQSSSYRHAHGSVLVVGTIPIR--------- 114

Query: 97  ACTYCSSFLVGIVESYCELWEICKFQESLHGTTSALECLLLLDEVEVPEGLVDICGSIVR 156
                               E+C FQ +  G T+ +ECLLL +E ++P+GL  +   IVR
Sbjct: 115 --------------------EVCMFQTNKQGMTTEIECLLLHEEAKIPQGLESLSIRIVR 174

Query: 157 VSPTVMKKLSGMQSTESIDAIALMRLPASFCSV-DHNEKEAHFKRWFPSPHRILVLEGIQ 216
           VS  VMKKLSG+QSTES++AIALMR+P+SF  + D  +      +WFPS HR+LVL+ IQ
Sbjct: 175 VSSLVMKKLSGVQSTESVEAIALMRIPSSFTDLKDDKDIITDCNKWFPSAHRVLVLDSIQ 234

Query: 217 DPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGACFQLPIVSGTWNHLETLKD 276
           DPGNLGTL+RSA+AF W+G FLLPGCCDP+N+KALRASRGA FQLPIVSG WNHL+ L++
Sbjct: 235 DPGNLGTLVRSAMAFNWDGAFLLPGCCDPYNDKALRASRGASFQLPIVSGNWNHLKLLEN 294

Query: 277 EFQMKMLAGHP--ESNEPKPVSFLTQQFANSLKDFPLCLVLGCEGSGLSEKSWQDCELLS 336
           EFQMK+LAGHP   + + KPVS L+ +FA SL + PLCL+LG EG+GLSE++ + C L+S
Sbjct: 295 EFQMKLLAGHPATTTQKLKPVSKLSVEFAQSLAEKPLCLILGSEGNGLSEQARKVCVLVS 349

Query: 337 IPMAGGFESLNVSVAGGIFLYMLQ 358
           IPMAG FESLNVSVAGGIFLYMLQ
Sbjct: 355 IPMAGDFESLNVSVAGGIFLYMLQ 349

BLAST of Sgr020673 vs. TAIR 10
Match: AT2G19870.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 54.3 bits (129), Expect = 2.4e-07
Identity = 55/172 (31.98%), Postives = 73/172 (42.44%), Query Frame = 0

Query: 209 LVLEGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGA--CFQLPIVSGT 268
           + L+ + DP NLG +IRSA  F   GV +      P +    +AS G+    +L      
Sbjct: 420 VALDEVTDPQNLGAIIRSAYFFGATGVVVCAKNSAPLSAVVSKASAGSLEVMELRYCKNM 479

Query: 269 WNHLETLKDEFQMKMLAGHPESNEPKPVSFLTQQFANSLKDFPLCLVLGCEGSGLS---E 328
              LE    E   +++ G   S  PK V+         L   P  LVLG EG+GL    E
Sbjct: 480 MQFLEA-SAENGWRVVGG---SVSPKAVA-----LNEVLPGSPTILVLGNEGTGLRPLVE 539

Query: 329 KSWQDCELLSIPMAG-------------GF------ESLNVSVAGGIFLYML 357
           +S  D   +S  M               GF      ESLNVSVA G+FL+ L
Sbjct: 540 RSCTDLVRISGNMPNEVAVTESDDAEGEGFRSFLAVESLNVSVAAGLFLHHL 582

BLAST of Sgr020673 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 52.4 bits (124), Expect = 9.0e-07
Identity = 44/150 (29.33%), Postives = 73/150 (48.67%), Query Frame = 0

Query: 209 LVLEGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGACFQLPIVSGTWN 268
           LV+EG+ D GN+    RSA A     V ++   CD  + K    +R     + + +  W 
Sbjct: 157 LVVEGLSDFGNISAAFRSADALGIQSVHVV--SCD--SSKRYNGNR----HVSMGAEKWL 216

Query: 269 HLE---TLKDEFQMKMLAGHPESNEPKPVSFLTQQFANSLKDFPLCLVLGCEGSGLSEKS 328
            +E   T K+ F++    G+  +     +   T    +     P  +V+G EG G+S+++
Sbjct: 217 DIEFWDTPKECFKVLKSRGYRIAT--THLGMDTVSIYDMDWSCPTAIVVGNEGRGISDEA 276

Query: 329 WQDCEL-LSIPMAGGFESLNVSVAGGIFLY 355
            +  +L  SIPM G  +S NVSVA GI ++
Sbjct: 277 LELSDLRCSIPMNGMVDSFNVSVAAGILMH 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136373.11.5e-16081.04uncharacterized protein LOC111008099 [Momordica charantia][more]
XP_038904874.19.0e-15076.44uncharacterized tRNA/rRNA methyltransferase slr1673 [Benincasa hispida][more]
XP_011650605.16.4e-14876.39uncharacterized protein LOC101218901 isoform X2 [Cucumis sativus] >KAE8650203.1 ... [more]
XP_031739071.18.4e-14876.39uncharacterized protein LOC101218901 isoform X1 [Cucumis sativus][more]
XP_008450171.17.1e-14776.67PREDICTED: uncharacterized tRNA/rRNA methyltransferase YsgA isoform X1 [Cucumis ... [more]
Match NameE-valueIdentityDescription
P945381.4e-1730.61Uncharacterized tRNA/rRNA methyltransferase YsgA OS=Bacillus subtilis (strain 16... [more]
P742611.8e-1536.08Uncharacterized tRNA/rRNA methyltransferase slr1673 OS=Synechocystis sp. (strain... [more]
A0QAB62.6e-1436.36Uncharacterized tRNA/rRNA methyltransferase MAV_0574 OS=Mycobacterium avium (str... [more]
Q9VW145.7e-1432.50rRNA methyltransferase 3, mitochondrial OS=Drosophila melanogaster OX=7227 GN=CG... [more]
Q743W25.7e-1435.33Uncharacterized tRNA/rRNA methyltransferase MAP_0479 OS=Mycolicibacterium paratu... [more]
Match NameE-valueIdentityDescription
A0A6J1C7F07.2e-16181.04uncharacterized protein LOC111008099 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A5D3DF853.4e-14776.67Putative tRNA/rRNA methyltransferase YsgA isoform X1 OS=Cucumis melo var. makuwa... [more]
A0A1S3BNM23.4e-14776.67uncharacterized tRNA/rRNA methyltransferase YsgA isoform X1 OS=Cucumis melo OX=3... [more]
A0A6J1FNI02.5e-14575.55uncharacterized protein LOC111447061 OS=Cucurbita moschata OX=3662 GN=LOC1114470... [more]
A0A6J1JVY61.2e-14475.27uncharacterized protein LOC111488367 OS=Cucurbita maxima OX=3661 GN=LOC111488367... [more]
Match NameE-valueIdentityDescription
AT4G38020.14.2e-10559.57tRNA/rRNA methyltransferase (SpoU) family protein [more]
AT2G19870.12.4e-0731.98tRNA/rRNA methyltransferase (SpoU) family protein [more]
AT5G15390.19.0e-0729.33tRNA/rRNA methyltransferase (SpoU) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001537tRNA/rRNA methyltransferase, SpoU typePFAMPF00588SpoU_methylasecoord: 208..354
e-value: 1.4E-28
score: 99.9
IPR029026tRNA (guanine-N1-)-methyltransferase, N-terminalGENE3D3.40.1280.10coord: 197..363
e-value: 1.8E-39
score: 137.0
NoneNo IPR availablePANTHERPTHR43191RRNA METHYLTRANSFERASE 3,coord: 48..361
NoneNo IPR availablePANTHERPTHR43191:SF2RRNA METHYLTRANSFERASE 3, MITOCHONDRIALcoord: 48..361
NoneNo IPR availableCDDcd18095SpoU-like_rRNA-MTasecoord: 207..356
e-value: 3.01602E-53
score: 170.685
IPR029028Alpha/beta knot methyltransferasesSUPERFAMILY75217alpha/beta knotcoord: 203..357

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr020673.1Sgr020673.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001510 RNA methylation
biological_process GO:0006396 RNA processing
molecular_function GO:0003723 RNA binding
molecular_function GO:0008173 RNA methyltransferase activity