Sgr020460 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr020460
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Pectinesterase
Location	tig00153533: 380412 .. 381993 (-)
RNA-Seq Expression	Sgr020460
Synteny	Sgr020460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATCAAAAGATGCAGCTTCTCCCTCCATTTCTTTTGTTCTTCACCTTGTCTAATGCTGCTGTTTTTGGTTCTGAAGCTGTGGCTTATGTAAGAAACTTTCAAGTTTTAAGAATTGAATCCACCATTGTGGTTGACAAGGCTGGCAGAGGCAATTTCCAAACCGTTCAAGCAGCTGTTGATTCAGTTCCATCGTATAACAACAAATGGATTAAGATTCAAATCAATCCTGGCGTATACAAGTAATACTAAACATGTGTATGCCTATTCTATGCTCCAAGCTCTCTGTATTTTCTGTTAAGGTTTTGCTCTAATATGGTTGGCAAACATTTTGCAGAGAAAAGGTGACGATCCCGGGGGACAAACCGTACATTTTTCTCGATGGAGATGACCGCAGGACTACGTTTATCACGTTCGATGACCATCAAAGTACAGATCTTAGTGCTACATTTACTTCAATGCCTGATAACATAGTTGCCAGAGGCATTACCTTTGAGGTGCTGCTGCTTCCATAACGTGATATAAACTGAACTTATATTCATATAATTTGAAATTTTAAAGCAAAGTAAGCTGCAAGTTTAAGGTCATAGTTGCATGAAAGATCGTACGCACCTGAGCAGTCGGGCAGGCTTCTATTGCTCAATCATTGATCAATGTCTTGTAAGAATTGTTGATTAGATATTGTTGTGGTGCATGCAGAACTCCTTCAACCTTGCAGAAGCTCCGCAACTGTACGATTACGAGGAAAGAACTTACATTACACAAGCAGTAGCTGCAAGAATTTTTGGTGACAGATCAGCATTTTTTGACTGCGGATTCATGGGTTTTCAAGACACATTATGGGATGTTCAAGGCCGCCATCTCTTCGAAAATTGCTATATCGAAGGTGCCATAGACTTCATCTTTGGTAGAGGCCAATCAATTTATAAGGTACTCAATAAACTTTCCAAGTTGATGCAAGAAAGCCATTCAAATTCTATTTCTGCCAACTTCACATGCTGATACTGTTATTACACAACTGAAGGAGACTTTTACTGCAATTAAAAACCAAAAATGCATGTTTTGACAATGCAGGACTGCTTGATAAACGTTAATGTCGCATCTCTCCCTCAAGTCCACCAAGGATACATTACTGCACAAGGTCGAGAATCGGAAGCAGATCCGAGTGGATTTATATTTAAACAGTGCAATGTAACAGGTGCTGGCCAAGTTCTTTTGGGGAGAGCTTATCGTCCTTATTCCAGAGTTATATTTGAGGATACAACATTGGGTTCAGTGGTAGCACCAGAGGGATGGGATGCCTGGAACTACAAGGGCCAAGAGTAAGTTCACAACCCAAAAAAGAAAAATAGCCTCTTGACCTTTAAGTTTTCTTGATTCAATAGCAAGTTCTCGTCATTTTCGTATTGGTGTTACATTCTTTAGGGAAAATTTTATGTATGTCGAGGTGAATGGTAGAGGGCCAGGATCTTCCACTTCCAAGAGAGTTCCATGGGAGAAGAAGCTTGGTGCCTCAGAACTAAGGAACTTCTCAATGGCATTCTTCATTAACCAAGATGGGTGGATTCCCACGATCCCTTGA

mRNA sequence

ATGAATCAAAAGATGCAGCTTCTCCCTCCATTTCTTTTGTTCTTCACCTTGTCTAATGCTGCTGTTTTTGGTTCTGAAGCTGTGGCTTATGTAAGAAACTTTCAAGTTTTAAGAATTGAATCCACCATTGTGGTTGACAAGGCTGGCAGAGGCAATTTCCAAACCGTTCAAGCAGCTGTTGATTCAGTTCCATCGTATAACAACAAATGGATTAAGATTCAAATCAATCCTGGCGTATACAAAGAAAAGGTGACGATCCCGGGGGACAAACCGTACATTTTTCTCGATGGAGATGACCGCAGGACTACGTTTATCACGTTCGATGACCATCAAAGTACAGATCTTAGTGCTACATTTACTTCAATGCCTGATAACATAGTTGCCAGAGGCATTACCTTTGAGAACTCCTTCAACCTTGCAGAAGCTCCGCAACTGTACGATTACGAGGAAAGAACTTACATTACACAAGCAGTAGCTGCAAGAATTTTTGGTGACAGATCAGCATTTTTTGACTGCGGATTCATGGGTTTTCAAGACACATTATGGGATGTTCAAGGCCGCCATCTCTTCGAAAATTGCTATATCGAAGGTGCCATAGACTTCATCTTTGGTAGAGGCCAATCAATTTATAAGGACTGCTTGATAAACGTTAATGTCGCATCTCTCCCTCAAGTCCACCAAGGATACATTACTGCACAAGGTCGAGAATCGGAAGCAGATCCGAGTGGATTTATATTTAAACAGTGCAATGTAACAGGTGCTGGCCAAGTTCTTTTGGGGAGAGCTTATCGTCCTTATTCCAGAGTTATATTTGAGGATACAACATTGGGTTCAGTGGTAGCACCAGAGGGATGGGATGCCTGGAACTACAAGGGCCAAGAGGAAAATTTTATGTATGTCGAGGTGAATGGTAGAGGGCCAGGATCTTCCACTTCCAAGAGAGTTCCATGGGAGAAGAAGCTTGGTGCCTCAGAACTAAGGAACTTCTCAATGGCATTCTTCATTAACCAAGATGGGTGGATTCCCACGATCCCTTGA

Coding sequence (CDS)

ATGAATCAAAAGATGCAGCTTCTCCCTCCATTTCTTTTGTTCTTCACCTTGTCTAATGCTGCTGTTTTTGGTTCTGAAGCTGTGGCTTATGTAAGAAACTTTCAAGTTTTAAGAATTGAATCCACCATTGTGGTTGACAAGGCTGGCAGAGGCAATTTCCAAACCGTTCAAGCAGCTGTTGATTCAGTTCCATCGTATAACAACAAATGGATTAAGATTCAAATCAATCCTGGCGTATACAAAGAAAAGGTGACGATCCCGGGGGACAAACCGTACATTTTTCTCGATGGAGATGACCGCAGGACTACGTTTATCACGTTCGATGACCATCAAAGTACAGATCTTAGTGCTACATTTACTTCAATGCCTGATAACATAGTTGCCAGAGGCATTACCTTTGAGAACTCCTTCAACCTTGCAGAAGCTCCGCAACTGTACGATTACGAGGAAAGAACTTACATTACACAAGCAGTAGCTGCAAGAATTTTTGGTGACAGATCAGCATTTTTTGACTGCGGATTCATGGGTTTTCAAGACACATTATGGGATGTTCAAGGCCGCCATCTCTTCGAAAATTGCTATATCGAAGGTGCCATAGACTTCATCTTTGGTAGAGGCCAATCAATTTATAAGGACTGCTTGATAAACGTTAATGTCGCATCTCTCCCTCAAGTCCACCAAGGATACATTACTGCACAAGGTCGAGAATCGGAAGCAGATCCGAGTGGATTTATATTTAAACAGTGCAATGTAACAGGTGCTGGCCAAGTTCTTTTGGGGAGAGCTTATCGTCCTTATTCCAGAGTTATATTTGAGGATACAACATTGGGTTCAGTGGTAGCACCAGAGGGATGGGATGCCTGGAACTACAAGGGCCAAGAGGAAAATTTTATGTATGTCGAGGTGAATGGTAGAGGGCCAGGATCTTCCACTTCCAAGAGAGTTCCATGGGAGAAGAAGCTTGGTGCCTCAGAACTAAGGAACTTCTCAATGGCATTCTTCATTAACCAAGATGGGTGGATTCCCACGATCCCTTGA

Protein sequence

MNQKMQLLPPFLLFFTLSNAAVFGSEAVAYVRNFQVLRIESTIVVDKAGRGNFQTVQAAVDSVPSYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDDRRTTFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDGWIPTIP

Homology

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BLAST of Sgr020460 vs. NCBI nr
Match: KAA0048077.1 (putative pectinesterase 66 [Cucumis melo var. makuwa])

HSP 1 Score: 538.9 bits (1387), Expect = 3.2e-149
Identity = 251/341 (73.61%), Postives = 290/341 (85.04%), Query Frame = 0


Query: 5   MQLLPPFLLFFTLSNAAVFGSEAVAYVRNFQVLRIESTIVVDKAGRGNFQTVQAAVDSVP 64
           MQL PPFLL FTL NA VFG EAV + R F  L ++S +VVDK+GRG+FQTVQAA+DSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 65  SYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDDRRTTFITFDDHQSTDLSATFTSMPD 124
             NN+WIKIQINPGVYKEKVTIP +KP+I+L+G DR  T ITFDDHQ TD SATFTS P 
Sbjct: 61  PNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPP 120

Query: 125 NIVARGITFENSFNLAEAPQLYDYEERTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 184
           NI+ARGITFENSFNL EAP+L+  ++ TYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 185 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGYITAQGRESEADPSGF 244
           QGRH F+NCYIEGAIDFIFG GQS+YKDCLINVNVASLPQVHQG+ITAQGR S ADPSGF
Sbjct: 181 QGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGF 240

Query: 245 IFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSVVAPEGWDAWNYKGQEENFMYVEVNG 304
           +FK C +TG+G+ LLGRAY P+SRVIF+D  +G VVAPEGWDAWN+K +E+NFMYVE N 
Sbjct: 241 VFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENC 300

Query: 305 RGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDGWIPTIP 346
            GPG+STS RVPW K L AS+LR FS+  FINQDGWIP +P
Sbjct: 301 TGPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 340

[Close]

BLAST of Sgr020460 vs. NCBI nr
Match: TYJ96459.1 (putative pectinesterase 66 [Cucumis melo var. makuwa])

HSP 1 Score: 538.1 bits (1385), Expect = 5.5e-149
Identity = 251/341 (73.61%), Postives = 290/341 (85.04%), Query Frame = 0


Query: 5   MQLLPPFLLFFTLSNAAVFGSEAVAYVRNFQVLRIESTIVVDKAGRGNFQTVQAAVDSVP 64
           MQL PPFLL FTL NA VFG EAV + R F  L ++S +VVDK+GRG+FQTVQAA+DSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 65  SYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDDRRTTFITFDDHQSTDLSATFTSMPD 124
             NN+WIKIQINPGVYKEKVTIP +KP+I+L+G DR  T ITFDDHQ TD SATFTS P 
Sbjct: 61  PNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPP 120

Query: 125 NIVARGITFENSFNLAEAPQLYDYEERTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 184
           NI+ARGITFENSFNL EAP+L+  ++ TYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 185 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGYITAQGRESEADPSGF 244
           QGRH F+NCYIEGAIDFIFG GQS+YKDCLINVNVASLPQVHQG+ITAQGR S ADPSGF
Sbjct: 181 QGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGF 240

Query: 245 IFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSVVAPEGWDAWNYKGQEENFMYVEVNG 304
           +FK C +TG+G+ LLGRAY P+SRVIF+D  +G VVAPEGWDAWN+K +E+NFMYVE N 
Sbjct: 241 VFKGCAITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENC 300

Query: 305 RGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDGWIPTIP 346
            GPG+STS RVPW K L AS+LR FS+  FINQDGWIP +P
Sbjct: 301 TGPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 340

[Close]

BLAST of Sgr020460 vs. NCBI nr
Match: KGN61008.1 (hypothetical protein Csa_021278 [Cucumis sativus])

HSP 1 Score: 523.1 bits (1346), Expect = 1.8e-144
Identity = 244/340 (71.76%), Postives = 285/340 (83.82%), Query Frame = 0


Query: 5   MQLLPPFLLFFTLSNAAVFGSEAVAYVRNFQVLRIESTIVVDKAGRGNFQTVQAAVDSVP 64
           MQ+ PPFLL FTL NA VFG EAV   R+F VL I+S ++VDK+G GNFQTVQAA+DSVP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDF-VLTIQSVVIVDKSGNGNFQTVQAAIDSVP 60

Query: 65  SYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDDRRTTFITFDDHQSTDLSATFTSMPD 124
             NN WIKIQINPGVYKEKVTIP +KP+I+L+G D   T ITFDDHQ TD SATFTS P 
Sbjct: 61  PNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPP 120

Query: 125 NIVARGITFENSFNLAEAPQLYDYEERTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 184
           NI+ RGITFENSFNL EAP+L+  ++ TYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 185 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGYITAQGRESEADPSGF 244
           QGRH F +CYIEGAIDFIFG GQS+Y+DC+INVNVASLPQV+QGYITAQ R+S ADPSGF
Sbjct: 181 QGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGF 240

Query: 245 IFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSVVAPEGWDAWNYKGQEENFMYVEVNG 304
           +FK+C + G+G+ LLGRAY P+SRVIF+D  +GSVVAPEGW AW++KG+EENFMYVE N 
Sbjct: 241 VFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENC 300

Query: 305 RGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDGWIPTI 345
            GPG+STS RVPW K L AS L  FS+  FINQDGWIPT+
Sbjct: 301 TGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTV 339

[Close]

BLAST of Sgr020460 vs. NCBI nr
Match: XP_016901459.1 (PREDICTED: probable pectinesterase 66 [Cucumis melo])

HSP 1 Score: 517.7 bits (1332), Expect = 7.7e-143
Identity = 250/371 (67.39%), Postives = 290/371 (78.17%), Query Frame = 0


Query: 5   MQLLPPFLLFFTLSNAAVFGSEAVAYVRNFQVLRIESTIVVDKAGRGNFQTVQAAVDSVP 64
           MQL PPFLL FTL NA VFG EAV + R F  L ++S +VVDK+GRG+FQTVQAA+DSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 65  SYNNKWIKIQINPGVY------KEKVTIPGDKPYIFLDGDDRRTTFITFDDHQSTDLSAT 124
             NN+WIKIQINPGVY      +EKVTIP +KP+I+L+G DR  T ITFDDHQ TD SAT
Sbjct: 61  PNNNQWIKIQINPGVYNWQTFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSAT 120

Query: 125 FTSMPDNIVARGITFE------------------------NSFNLAEAPQLYDYEERTYI 184
           FTS P NI+ARGITFE                        NSFNL EAP+L+  ++ TYI
Sbjct: 121 FTSRPPNIIARGITFEVLWLLKTEFIFIASFEILKPKCTQNSFNLEEAPELFVCDDGTYI 180

Query: 185 TQAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCL 244
           TQA+AARI+GD+SAFF+CGF G+QDTLWDVQGRH F+NCYIEGAIDFIFG GQS+YKDCL
Sbjct: 181 TQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCL 240

Query: 245 INVNVASLPQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDT 304
           INVNVASLPQVHQG+ITAQGR S ADPSGF+FK C +TG+G+ LLGRAY P+SRVIF+D 
Sbjct: 241 INVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDA 300

Query: 305 TLGSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFF 346
            +G VVAPEGWDAWN+K +E+NFMYVE N  GPG+STS RVPW K L AS+LR FS+  F
Sbjct: 301 IMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESF 360

[Close]

BLAST of Sgr020460 vs. NCBI nr
Match: XP_031736683.1 (probable pectinesterase 66 [Cucumis sativus])

HSP 1 Score: 510.4 bits (1313), Expect = 1.2e-140
Identity = 244/362 (67.40%), Postives = 285/362 (78.73%), Query Frame = 0


Query: 5   MQLLPPFLLFFTLSNAAVFGSEAVAYVRNFQVLRIESTIVVDKAGRGNFQTVQAAVDSVP 64
           MQ+ PPFLL FTL NA VFG EAV   R+F VL I+S ++VDK+G GNFQTVQAA+DSVP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDF-VLTIQSVVIVDKSGNGNFQTVQAAIDSVP 60

Query: 65  SYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDDRRTTFITFDDHQSTDLSATFTSMPD 124
             NN WIKIQINPGVYKEKVTIP +KP+I+L+G D   T ITFDDHQ TD SATFTS P 
Sbjct: 61  PNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPP 120

Query: 125 NIVARGITFE----------------------NSFNLAEAPQLYDYEERTYITQAVAARI 184
           NI+ RGITFE                      NSFNL EAP+L+  ++ TYITQA+AARI
Sbjct: 121 NIIVRGITFEVLWLLKTDFIFIALFEILKLCKNSFNLREAPELFSCDDGTYITQAIAARI 180

Query: 185 FGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASL 244
           +GD+SAFF+CGF G+QDTLWDVQGRH F +CYIEGAIDFIFG GQS+Y+DC+INVNVASL
Sbjct: 181 YGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASL 240

Query: 245 PQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSVVAP 304
           PQV+QGYITAQ R+S ADPSGF+FK+C + G+G+ LLGRAY P+SRVIF+D  +GSVVAP
Sbjct: 241 PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAP 300

Query: 305 EGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDGWIP 345
           EGW AW++KG+EENFMYVE N  GPG+STS RVPW K L AS L  FS+  FINQDGWIP
Sbjct: 301 EGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIP 360

[Close]

BLAST of Sgr020460 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 303.9 bits (777), Expect = 2.3e-81
Identity = 148/309 (47.90%), Postives = 196/309 (63.43%), Query Frame = 0


Query: 38  RIESTIVVDKAGRGNFQTVQAAVDSVPSYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDG 97
           +I  TI VD  G GNF TVQ+A+DS+   N+ WI++    G+Y+EKVTIP +K +I+L G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 98  DDRRTTFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTYITQA 157
                T I +DDHQ+TD+SATFT+  D+IV  GITF+N++N+           +  I  A
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVP-------NNKREIVPA 149

Query: 158 VAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINV 217
           VAAR+ GDR    D  F+G QDTL+D +GRH ++ C I G IDFIFG GQS++K+C +N+
Sbjct: 150 VAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNM 209

Query: 218 NVA-SLPQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTL 277
            +    P    G ITA  R S +D  GF+F  C VTG G+ LLGRA+   +RVIF+ + L
Sbjct: 210 TLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRL 269

Query: 278 GSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFIN 337
             VV P GWDAW  KG E +  +VE    G G+ TS+RVPW KKL  SE+  F+   FI+
Sbjct: 270 SDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFID 329

Query: 338 QDGWIPTIP 346
           QDGWI   P
Sbjct: 330 QDGWISRFP 331

[Close]

BLAST of Sgr020460 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-80
Identity = 144/306 (47.06%), Postives = 190/306 (62.09%), Query Frame = 0


Query: 40  ESTIVVDKAGRGNFQTVQAAVDSVPSYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDD 99
           +  + VD++G GNF T+Q A+DSVP  N  W  I +  G+Y+EK+ IP +KP+I L G  
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 100 RRTTFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTYITQAVA 159
           +R T + +DDH S   S TF+++ DN V + ITF NS+N     ++     +   T AVA
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 160 ARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNV 219
           A I GD+SAF+  GF G QDTLWD  GRH F  C I+GA+DFIFG GQSIY+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 220 ASLPQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSV 279
             L     GYITAQGR +  D +GFIF  C V G G   LGR +R YSRVIF ++ L  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 280 VAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDG 339
           V PEGWDAWN+ G E   ++ E    G G++  +RV W KKL  S ++N +   FIN+ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 330

Query: 340 WIPTIP 346
           W+  +P
Sbjct: 331 WVEDLP 332

[Close]

BLAST of Sgr020460 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-79
Identity = 144/303 (47.52%), Postives = 191/303 (63.04%), Query Frame = 0


Query: 43  IVVDKAGRGNFQTVQAAVDSVPSYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDDRRT 102
           ++VD++G GNF T+Q A+DSVP  N  W  I +  G+Y+EK+TIP  KP+I + G  +R+
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 103 TFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTYITQAVAARI 162
           T + +DDH S   S TF ++ DN V + ITF NS+N     ++     +     AVAA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 163 FGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASL 222
            GD+SAF+  GF G QDTLWD  GRH F  C I+GA+DFI G GQSIY+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 223 PQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSVVAP 282
                GYITAQGR +  D +GF+F  C V G G+  LGRA+RPYSRVIF ++ L  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 283 EGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDGWIP 342
            GW  WNY+G E+   Y E    G GS+TS+R  W KKL AS +++ +   FIN+ GW+ 
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVE 327

Query: 343 TIP 346
            +P
Sbjct: 329 DLP 327

[Close]

BLAST of Sgr020460 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.0e-76
Identity = 141/309 (45.63%), Postives = 189/309 (61.17%), Query Frame = 0


Query: 38  RIESTIVVDKAGRGNFQTVQAAVDSVPSYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDG 97
           +I  TI+V+      F+TVQ+A+DS+P  N  WI+I I+ G+Y EKVTIP  K YI++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 98  DDRRTTFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTYITQA 157
                T I + DHQ T+ SATFTS P NI+  GITF+N +N+A +         +    A
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS--------SSPTKPA 155

Query: 158 VAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINV 217
           VAA + GD+ A  D  F GFQDTL+D  GRH ++ C I G IDFIFG  QSI++ C + +
Sbjct: 156 VAAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKL 215

Query: 218 NVASLPQVH-QGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTL 277
            V   P     G ITAQGR+S  D  GF+FK C V G+G+ LLGRA++ YSRVIF  +  
Sbjct: 216 RVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMF 275

Query: 278 GSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFIN 337
              + P GWDAW  KGQE +  +VE    G G+ TSKRVPW  K    ++  F+   FI+
Sbjct: 276 SDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFID 335

Query: 338 QDGWIPTIP 346
           ++GW+  +P
Sbjct: 336 EEGWLSRLP 336

[Close]

BLAST of Sgr020460 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 262.7 bits (670), Expect = 5.9e-69
Identity = 130/252 (51.59%), Postives = 174/252 (69.05%), Query Frame = 0


Query: 97  GDDRRTTFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTY-IT 156
           G+ +R T IT++ H +TD+S+TFTS P +IV R ++  N++N      L      ++ I 
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIK 101

Query: 157 QAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLI 216
            AVA  ++GD+SAF++C F+G QDT+WD  GRH F+NCYIEGAIDFIFG GQS+Y+DC I
Sbjct: 102 PAVAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHI 161

Query: 217 NVNVASL-PQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDT 276
           N    +L  +V  GYITAQGR S++DPSGF+F + +V+G+  V LGRAY P+SRVIF  T
Sbjct: 162 NATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQT 221

Query: 277 TLGSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELR-NFSMAF 336
            L SVV PEGW +W+Y G E +F Y EV  +G GS  S+RVPW  KL +   +  FS++ 
Sbjct: 222 DLSSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISN 281

Query: 337 FINQDGWIPTIP 346
           FI+QD WI  IP
Sbjct: 282 FIDQDQWISNIP 291

[Close]

BLAST of Sgr020460 vs. ExPASy TrEMBL
Match: A0A5A7U1G9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001480 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.6e-149
Identity = 251/341 (73.61%), Postives = 290/341 (85.04%), Query Frame = 0


Query: 5   MQLLPPFLLFFTLSNAAVFGSEAVAYVRNFQVLRIESTIVVDKAGRGNFQTVQAAVDSVP 64
           MQL PPFLL FTL NA VFG EAV + R F  L ++S +VVDK+GRG+FQTVQAA+DSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 65  SYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDDRRTTFITFDDHQSTDLSATFTSMPD 124
             NN+WIKIQINPGVYKEKVTIP +KP+I+L+G DR  T ITFDDHQ TD SATFTS P 
Sbjct: 61  PNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPP 120

Query: 125 NIVARGITFENSFNLAEAPQLYDYEERTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 184
           NI+ARGITFENSFNL EAP+L+  ++ TYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 185 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGYITAQGRESEADPSGF 244
           QGRH F+NCYIEGAIDFIFG GQS+YKDCLINVNVASLPQVHQG+ITAQGR S ADPSGF
Sbjct: 181 QGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGF 240

Query: 245 IFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSVVAPEGWDAWNYKGQEENFMYVEVNG 304
           +FK C +TG+G+ LLGRAY P+SRVIF+D  +G VVAPEGWDAWN+K +E+NFMYVE N 
Sbjct: 241 VFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENC 300

Query: 305 RGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDGWIPTIP 346
            GPG+STS RVPW K L AS+LR FS+  FINQDGWIP +P
Sbjct: 301 TGPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 340

[Close]

BLAST of Sgr020460 vs. ExPASy TrEMBL
Match: A0A5D3BBR9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001020 PE=4 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.7e-149
Identity = 251/341 (73.61%), Postives = 290/341 (85.04%), Query Frame = 0


Query: 5   MQLLPPFLLFFTLSNAAVFGSEAVAYVRNFQVLRIESTIVVDKAGRGNFQTVQAAVDSVP 64
           MQL PPFLL FTL NA VFG EAV + R F  L ++S +VVDK+GRG+FQTVQAA+DSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 65  SYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDDRRTTFITFDDHQSTDLSATFTSMPD 124
             NN+WIKIQINPGVYKEKVTIP +KP+I+L+G DR  T ITFDDHQ TD SATFTS P 
Sbjct: 61  PNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPP 120

Query: 125 NIVARGITFENSFNLAEAPQLYDYEERTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 184
           NI+ARGITFENSFNL EAP+L+  ++ TYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 185 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGYITAQGRESEADPSGF 244
           QGRH F+NCYIEGAIDFIFG GQS+YKDCLINVNVASLPQVHQG+ITAQGR S ADPSGF
Sbjct: 181 QGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGF 240

Query: 245 IFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSVVAPEGWDAWNYKGQEENFMYVEVNG 304
           +FK C +TG+G+ LLGRAY P+SRVIF+D  +G VVAPEGWDAWN+K +E+NFMYVE N 
Sbjct: 241 VFKGCAITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENC 300

Query: 305 RGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDGWIPTIP 346
            GPG+STS RVPW K L AS+LR FS+  FINQDGWIP +P
Sbjct: 301 TGPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 340

[Close]

BLAST of Sgr020460 vs. ExPASy TrEMBL
Match: A0A0A0LH00 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G034610 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 8.9e-145
Identity = 244/340 (71.76%), Postives = 285/340 (83.82%), Query Frame = 0


Query: 5   MQLLPPFLLFFTLSNAAVFGSEAVAYVRNFQVLRIESTIVVDKAGRGNFQTVQAAVDSVP 64
           MQ+ PPFLL FTL NA VFG EAV   R+F VL I+S ++VDK+G GNFQTVQAA+DSVP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDF-VLTIQSVVIVDKSGNGNFQTVQAAIDSVP 60

Query: 65  SYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDDRRTTFITFDDHQSTDLSATFTSMPD 124
             NN WIKIQINPGVYKEKVTIP +KP+I+L+G D   T ITFDDHQ TD SATFTS P 
Sbjct: 61  PNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPP 120

Query: 125 NIVARGITFENSFNLAEAPQLYDYEERTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 184
           NI+ RGITFENSFNL EAP+L+  ++ TYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 185 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGYITAQGRESEADPSGF 244
           QGRH F +CYIEGAIDFIFG GQS+Y+DC+INVNVASLPQV+QGYITAQ R+S ADPSGF
Sbjct: 181 QGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGF 240

Query: 245 IFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSVVAPEGWDAWNYKGQEENFMYVEVNG 304
           +FK+C + G+G+ LLGRAY P+SRVIF+D  +GSVVAPEGW AW++KG+EENFMYVE N 
Sbjct: 241 VFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENC 300

Query: 305 RGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDGWIPTI 345
            GPG+STS RVPW K L AS L  FS+  FINQDGWIPT+
Sbjct: 301 TGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTV 339

[Close]

BLAST of Sgr020460 vs. ExPASy TrEMBL
Match: A0A1S4DZS3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499379 PE=4 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 3.7e-143
Identity = 250/371 (67.39%), Postives = 290/371 (78.17%), Query Frame = 0


Query: 5   MQLLPPFLLFFTLSNAAVFGSEAVAYVRNFQVLRIESTIVVDKAGRGNFQTVQAAVDSVP 64
           MQL PPFLL FTL NA VFG EAV + R F  L ++S +VVDK+GRG+FQTVQAA+DSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 65  SYNNKWIKIQINPGVY------KEKVTIPGDKPYIFLDGDDRRTTFITFDDHQSTDLSAT 124
             NN+WIKIQINPGVY      +EKVTIP +KP+I+L+G DR  T ITFDDHQ TD SAT
Sbjct: 61  PNNNQWIKIQINPGVYNWQTFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSAT 120

Query: 125 FTSMPDNIVARGITFE------------------------NSFNLAEAPQLYDYEERTYI 184
           FTS P NI+ARGITFE                        NSFNL EAP+L+  ++ TYI
Sbjct: 121 FTSRPPNIIARGITFEVLWLLKTEFIFIASFEILKPKCTQNSFNLEEAPELFVCDDGTYI 180

Query: 185 TQAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCL 244
           TQA+AARI+GD+SAFF+CGF G+QDTLWDVQGRH F+NCYIEGAIDFIFG GQS+YKDCL
Sbjct: 181 TQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCL 240

Query: 245 INVNVASLPQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDT 304
           INVNVASLPQVHQG+ITAQGR S ADPSGF+FK C +TG+G+ LLGRAY P+SRVIF+D 
Sbjct: 241 INVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDA 300

Query: 305 TLGSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFF 346
            +G VVAPEGWDAWN+K +E+NFMYVE N  GPG+STS RVPW K L AS+LR FS+  F
Sbjct: 301 IMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESF 360

[Close]

BLAST of Sgr020460 vs. ExPASy TrEMBL
Match: A0A6J1G5A0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450826 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.7e-125
Identity = 209/306 (68.30%), Postives = 251/306 (82.03%), Query Frame = 0


Query: 37  LRIESTIVVDKAGRGNFQTVQAAVDSVPSYNNKWIKIQINPGVYKEKVTIPGDKPYIFLD 96
           L+I+S IVVDK+G+GNF+T+QAA+DSVP  NNKW+KIQ+NPG Y+E VTIP  K +I+L 
Sbjct: 14  LKIQSVIVVDKSGKGNFKTIQAAIDSVPLNNNKWVKIQVNPGEYREMVTIPSTKSFIYLQ 73

Query: 97  GDDRRTTFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTYITQ 156
           G  R TT ITF+ HQ T+ SATF SM DNI+ARGITFENSFNLA APQL+D    T+ TQ
Sbjct: 74  GGSRSTTVITFNGHQQTNTSATFNSMADNIIARGITFENSFNLANAPQLFDKITGTFFTQ 133

Query: 157 AVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLIN 216
           A+AARI+GD+SAF  CGF GFQDTLWDV+GRH F++CYIEGAIDFIFG GQSIY+ CLI 
Sbjct: 134 ALAARIYGDKSAFISCGFKGFQDTLWDVKGRHYFKSCYIEGAIDFIFGNGQSIYEGCLIY 193

Query: 217 VNVASLPQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTL 276
           VNV  LP+V+QGYITAQGR+S  DPSGF+F+QC +TG+G+  LGRAY P+SRVIF+DT +
Sbjct: 194 VNVGLLPEVNQGYITAQGRQSANDPSGFVFQQCTITGSGKGFLGRAYGPFSRVIFKDTNM 253

Query: 277 GSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFIN 336
           G VVAPEGWDAW++KGQE NF+Y+E N RG GSS SKRVPW    G S+L  +S+  FIN
Sbjct: 254 GKVVAPEGWDAWHFKGQETNFVYIESNSRGIGSSPSKRVPWAMTQGNSQLSGYSVKSFIN 313

Query: 337 QDGWIP 343
           QDGWIP
Sbjct: 314 QDGWIP 319

[Close]

BLAST of Sgr020460 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 1.6e-82
Identity = 148/309 (47.90%), Postives = 196/309 (63.43%), Query Frame = 0


Query: 38  RIESTIVVDKAGRGNFQTVQAAVDSVPSYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDG 97
           +I  TI VD  G GNF TVQ+A+DS+   N+ WI++    G+Y+EKVTIP +K +I+L G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 98  DDRRTTFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTYITQA 157
                T I +DDHQ+TD+SATFT+  D+IV  GITF+N++N+           +  I  A
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVP-------NNKREIVPA 149

Query: 158 VAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINV 217
           VAAR+ GDR    D  F+G QDTL+D +GRH ++ C I G IDFIFG GQS++K+C +N+
Sbjct: 150 VAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNM 209

Query: 218 NVA-SLPQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTL 277
            +    P    G ITA  R S +D  GF+F  C VTG G+ LLGRA+   +RVIF+ + L
Sbjct: 210 TLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRL 269

Query: 278 GSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFIN 337
             VV P GWDAW  KG E +  +VE    G G+ TS+RVPW KKL  SE+  F+   FI+
Sbjct: 270 SDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFID 329

Query: 338 QDGWIPTIP 346
           QDGWI   P
Sbjct: 330 QDGWISRFP 331

[Close]

BLAST of Sgr020460 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 1.1e-81
Identity = 144/306 (47.06%), Postives = 190/306 (62.09%), Query Frame = 0


Query: 40  ESTIVVDKAGRGNFQTVQAAVDSVPSYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDD 99
           +  + VD++G GNF T+Q A+DSVP  N  W  I +  G+Y+EK+ IP +KP+I L G  
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 100 RRTTFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTYITQAVA 159
           +R T + +DDH S   S TF+++ DN V + ITF NS+N     ++     +   T AVA
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 160 ARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNV 219
           A I GD+SAF+  GF G QDTLWD  GRH F  C I+GA+DFIFG GQSIY+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 220 ASLPQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSV 279
             L     GYITAQGR +  D +GFIF  C V G G   LGR +R YSRVIF ++ L  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 280 VAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDG 339
           V PEGWDAWN+ G E   ++ E    G G++  +RV W KKL  S ++N +   FIN+ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 330

Query: 340 WIPTIP 346
           W+  +P
Sbjct: 331 WVEDLP 332

[Close]

BLAST of Sgr020460 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.2e-80
Identity = 144/303 (47.52%), Postives = 191/303 (63.04%), Query Frame = 0


Query: 43  IVVDKAGRGNFQTVQAAVDSVPSYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDGDDRRT 102
           ++VD++G GNF T+Q A+DSVP  N  W  I +  G+Y+EK+TIP  KP+I + G  +R+
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 103 TFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTYITQAVAARI 162
           T + +DDH S   S TF ++ DN V + ITF NS+N     ++     +     AVAA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 163 FGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASL 222
            GD+SAF+  GF G QDTLWD  GRH F  C I+GA+DFI G GQSIY+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 223 PQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTLGSVVAP 282
                GYITAQGR +  D +GF+F  C V G G+  LGRA+RPYSRVIF ++ L  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 283 EGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFINQDGWIP 342
            GW  WNY+G E+   Y E    G GS+TS+R  W KKL AS +++ +   FIN+ GW+ 
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVE 327

Query: 343 TIP 346
            +P
Sbjct: 329 DLP 327

[Close]

BLAST of Sgr020460 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 7.1e-78
Identity = 141/309 (45.63%), Postives = 189/309 (61.17%), Query Frame = 0


Query: 38  RIESTIVVDKAGRGNFQTVQAAVDSVPSYNNKWIKIQINPGVYKEKVTIPGDKPYIFLDG 97
           +I  TI+V+      F+TVQ+A+DS+P  N  WI+I I+ G+Y EKVTIP  K YI++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 98  DDRRTTFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTYITQA 157
                T I + DHQ T+ SATFTS P NI+  GITF+N +N+A +         +    A
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS--------SSPTKPA 155

Query: 158 VAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINV 217
           VAA + GD+ A  D  F GFQDTL+D  GRH ++ C I G IDFIFG  QSI++ C + +
Sbjct: 156 VAAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKL 215

Query: 218 NVASLPQVH-QGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDTTL 277
            V   P     G ITAQGR+S  D  GF+FK C V G+G+ LLGRA++ YSRVIF  +  
Sbjct: 216 RVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMF 275

Query: 278 GSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELRNFSMAFFIN 337
              + P GWDAW  KGQE +  +VE    G G+ TSKRVPW  K    ++  F+   FI+
Sbjct: 276 SDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFID 335

Query: 338 QDGWIPTIP 346
           ++GW+  +P
Sbjct: 336 EEGWLSRLP 336

[Close]

BLAST of Sgr020460 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 4.2e-70
Identity = 130/252 (51.59%), Postives = 174/252 (69.05%), Query Frame = 0


Query: 97  GDDRRTTFITFDDHQSTDLSATFTSMPDNIVARGITFENSFNLAEAPQLYDYEERTY-IT 156
           G+ +R T IT++ H +TD+S+TFTS P +IV R ++  N++N      L      ++ I 
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIK 101

Query: 157 QAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLI 216
            AVA  ++GD+SAF++C F+G QDT+WD  GRH F+NCYIEGAIDFIFG GQS+Y+DC I
Sbjct: 102 PAVAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHI 161

Query: 217 NVNVASL-PQVHQGYITAQGRESEADPSGFIFKQCNVTGAGQVLLGRAYRPYSRVIFEDT 276
           N    +L  +V  GYITAQGR S++DPSGF+F + +V+G+  V LGRAY P+SRVIF  T
Sbjct: 162 NATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQT 221

Query: 277 TLGSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTSKRVPWEKKLGASELR-NFSMAF 336
            L SVV PEGW +W+Y G E +F Y EV  +G GS  S+RVPW  KL +   +  FS++ 
Sbjct: 222 DLSSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISN 281

Query: 337 FINQDGWIPTIP 346
           FI+QD WI  IP
Sbjct: 282 FIDQDQWISNIP 291

The following BLAST results are available for this feature:

BLAST of Sgr020460 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0048077.1	3.2e-149	73.61	putative pectinesterase 66 [Cucumis melo var. makuwa]	[more]
TYJ96459.1	5.5e-149	73.61	putative pectinesterase 66 [Cucumis melo var. makuwa]	[more]
KGN61008.1	1.8e-144	71.76	hypothetical protein Csa_021278 [Cucumis sativus]	[more]
XP_016901459.1	7.7e-143	67.39	PREDICTED: probable pectinesterase 66 [Cucumis melo]	[more]
XP_031736683.1	1.2e-140	67.40	probable pectinesterase 66 [Cucumis sativus]	[more]

BLAST of Sgr020460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4PSQ5	2.3e-81	47.90	Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2	[more]
Q4PSN0	1.5e-80	47.06	Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1	[more]
Q3E9D3	1.6e-79	47.52	Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1	[more]
O64479	1.0e-76	45.63	Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1	[more]
O04953	5.9e-69	51.59	Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2	[more]

BLAST of Sgr020460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U1G9	1.6e-149	73.61	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00148...	[more]
A0A5D3BBR9	2.7e-149	73.61	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00102...	[more]
A0A0A0LH00	8.9e-145	71.76	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G034610 PE=4 SV=1	[more]
A0A1S4DZS3	3.7e-143	67.39	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499379 PE=4 SV=1	[more]
A0A6J1G5A0	2.7e-125	68.30	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450826 PE=4 SV=1	[more]

BLAST of Sgr020460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47280.1	1.6e-82	47.90	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	1.1e-81	47.06	Pectin lyase-like superfamily protein	[more]
AT5G18990.1	1.2e-80	47.52	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	7.1e-78	45.63	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	4.2e-70	51.59	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 43..337 e-value: 2.9E-62 score: 210.1
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 36..344 e-value: 1.4E-101 score: 342.1
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 8..345
None	No IPR available	PANTHER	PTHR31321:SF82	PECTINESTERASE	coord: 8..345
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 195..204
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 40..343

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr020460.1	Sgr020460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity