Sgr019931 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr019931 vs. NCBI nr
Match: XP_038893171.1 (putative pectinesterase/pectinesterase inhibitor 45 [Benincasa hispida])

HSP 1 Score: 928.3 bits (2398), Expect = 3.3e-266
Identity = 472/595 (79.33%), Postives = 523/595 (87.90%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV----SSKSGT----- 60
           MVFQDFDQISERRRAER RKFRKRVTIAVVS  V+LL++AAAV V    SSKS T     
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSVSVILLVIAAAVFVQVSSSSKSTTKNGNS 60

Query: 61  ------ANAKQPQTPATKEVSRVEKLISMICNSTDYKGKCESTLKDGVQSNPQSSDPKDL 120
                  N+K+      K+VSRVEK+I+MICNSTDYKGKCESTLKDG+ ++  SSDPKDL
Sbjct: 61  KSSSSNENSKETVPDDVKQVSRVEKMITMICNSTDYKGKCESTLKDGIHTDSNSSDPKDL 120

Query: 121 LKVAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELEKSVTQVSNNADL 180
           +K++ISAA DEVK+AVKK T FNF   EEK AFEDCKVL EDA+EELE S++QVS   ++
Sbjct: 121 IKLSISAAADEVKSAVKKATGFNFATAEEKGAFEDCKVLLEDAVEELEMSMSQVSIK-NM 180

Query: 181 KKLTSR-TPDLNNWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELTSNCLAMISQVA 240
            KLT++ TPDLNNWLSAVMSY ETCVDGFPEGK+K++MEK +K  KELTSN LAMISQVA
Sbjct: 181 GKLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKIKTEMEKVVKNGKELTSNSLAMISQVA 240

Query: 241 SFFTSFEMPEGAS--RHLLAHDGFPTWMTHEERRMLKGAPA-DKPKPNVVVAKDGSGDFK 300
           SFF++FEMPEGA+  R L+A +GFPTWM   ERRMLKGA A DKPKPNVVVAKDGSGDFK
Sbjct: 241 SFFSTFEMPEGAATRRRLMASNGFPTWMNSNERRMLKGATAGDKPKPNVVVAKDGSGDFK 300

Query: 301 TISQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMITGNKNFVDG 360
           TI++ALAAMP KY+GRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMI+GNKNFVDG
Sbjct: 301 TINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMISGNKNFVDG 360

Query: 361 VRTFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYA 420
           VRTFQTATF ALG+GF AQ IGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLY 
Sbjct: 361 VRTFQTATFVALGEGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYV 420

Query: 421 QAHRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHETTGIV 480
           QAHRQFYRSCLITGTIDFIFGDAAA FQNCN+MVRKPL+NQQNIVTAQGRTD+HETTGIV
Sbjct: 421 QAHRQFYRSCLITGTIDFIFGDAAAFFQNCNMMVRKPLDNQQNIVTAQGRTDKHETTGIV 480

Query: 481 LQNCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDFALST 540
           LQNC+I PDKTLEPVKSQFKSYLGRPWKEFSRT+VMESTIEDIIHPDGW+PWEGDFAL T
Sbjct: 481 LQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKT 540

Query: 541 LFYAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVDWIESKSVPFHL 577
           L+YAEFNN+G GA+T+ RV+WPGYKVIDK+EA KFTIG+FLE+DWIES S P H+
Sbjct: 541 LYYAEFNNQGPGAKTDGRVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHV 594

BLAST of Sgr019931 vs. NCBI nr
Match: XP_004142361.1 (putative pectinesterase/pectinesterase inhibitor 45 [Cucumis sativus] >KGN52371.1 hypothetical protein Csa_008584 [Cucumis sativus])

HSP 1 Score: 926.8 bits (2394), Expect = 9.6e-266
Identity = 467/592 (78.89%), Postives = 525/592 (88.68%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV---SSKSGT------ 60
           MVFQDFDQISERRRAER RKFRKRV IAVVS  V+LL+VAAAV V   SSKS T      
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQVSSSKSTTKNDDSG 60

Query: 61  ---ANAKQPQTPATKEVSRVEKLISMICNSTDYKGKCESTLKDGVQSNPQSSDPKDLLKV 120
               N+KQP  P TK+VSRVEK+I+MICNSTDYKGKCESTLKDGV ++P SSDPKDL+K+
Sbjct: 61  SSSGNSKQPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGVHTDPNSSDPKDLIKL 120

Query: 121 AISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELEKSVTQVSNNADLKKL 180
           AISAA  EVK+AVKK + FNF  PEEK AFEDCKVL EDA+EELE S+++V N  ++ KL
Sbjct: 121 AISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAIEELEMSMSEV-NKKNMGKL 180

Query: 181 TSR-TPDLNNWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELTSNCLAMISQVASFF 240
           T++ TP+LNNWLSAVMSY ETCVDGFPEGK+KSD+EK +KA KELTSN LAMISQVASFF
Sbjct: 181 TAKTTPNLNNWLSAVMSYHETCVDGFPEGKMKSDIEKVVKAGKELTSNSLAMISQVASFF 240

Query: 241 TSFEMPEGAS--RHLLAHDGFPTWMTHEERRMLKGAPA-DKPKPNVVVAKDGSGDFKTIS 300
           ++FEMPEGA+  R L+  +G PTWM   +RRMLKGA A +KPKPNVVVAKDGSG+FKTI+
Sbjct: 241 STFEMPEGAASRRRLMTTNGVPTWMDRNQRRMLKGAAAGEKPKPNVVVAKDGSGEFKTIN 300

Query: 301 QALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMITGNKNFVDGVRT 360
           +ALAAMP KY+GRYVIYVKEGIY+ETVVITKKMVNVTMYGDGSQKSMI+G+KNFVDGVRT
Sbjct: 301 EALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVRT 360

Query: 361 FQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYAQAH 420
           FQTATF ALG+GF+ Q IGFRN AGPEKHQAVAARVQADRAIF+NCRFEGYQDTLY QAH
Sbjct: 361 FQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYTQAH 420

Query: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHETTGIVLQN 480
           RQFYRSCLITGTIDFIFGDAAAIFQNCN+M+RKPL+NQQN+VTAQGRTD+HETTGIVLQN
Sbjct: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNMVTAQGRTDKHETTGIVLQN 480

Query: 481 CRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDFALSTLFY 540
           C+I PDKTLEPVKSQFKSYLGRPWKEFSRT+VMESTIED+IHPDGW+ WEGDFAL TL+Y
Sbjct: 481 CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDVIHPDGWMAWEGDFALKTLYY 540

Query: 541 AEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVDWIESKSVPFHL 577
           AEFNNKG GA+T+ARV+WPGYKVIDK+EA KFTIG+FLE+DWIES S P H+
Sbjct: 541 AEFNNKGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHV 591

BLAST of Sgr019931 vs. NCBI nr
Match: XP_008460558.1 (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo] >KAA0045465.1 putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo var. makuwa] >TYK00244.1 putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo var. makuwa])

HSP 1 Score: 924.5 bits (2388), Expect = 4.8e-265
Identity = 465/593 (78.41%), Postives = 523/593 (88.20%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV-------------SS 60
           MVFQDFDQISERRRAER RKFRKRVTIAVVS  V+LL+VAAAV V              S
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVSSSSKSTTKNGDS 60

Query: 61  KSGTANAKQPQTPATKEVSRVEKLISMICNSTDYKGKCESTLKDGVQSNPQSSDPKDLLK 120
            S T N+K+P  P TK+VSRVEK+I+MICNSTDYKGKCESTLKDG+ ++P SSDPKDL+K
Sbjct: 61  GSSTGNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPNSSDPKDLIK 120

Query: 121 VAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELEKSVTQVSNNADLKK 180
           +AISAA  EVK+AVKK + FNF  PEEK AFEDCKVL EDA+EELE S+++VS   ++ K
Sbjct: 121 LAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKK-NMGK 180

Query: 181 LTSR-TPDLNNWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELTSNCLAMISQVASF 240
           LT++ TPDLNNWLSAVMSY ETCVDGFPEGK+KS+MEK +KA KELTSN LAMISQVASF
Sbjct: 181 LTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMKSEMEKVVKAGKELTSNSLAMISQVASF 240

Query: 241 FTSFEMPEGAS--RHLLAHDGFPTWMTHEERRMLKGAPA-DKPKPNVVVAKDGSGDFKTI 300
           F++FEMPEGA+  R L+A  G PTWM   ERRMLKGA A +KPKPNVVVAKDGSG+FKTI
Sbjct: 241 FSTFEMPEGAASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVVVAKDGSGEFKTI 300

Query: 301 SQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMITGNKNFVDGVR 360
           ++ALAAMP KY+GRYVIYVKEGIY+ETVVITKKMVNVTMYGDGSQKSMI+G+KNFVDGVR
Sbjct: 301 NEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVR 360

Query: 361 TFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYAQA 420
           TFQTATF ALG+GF+ Q IGFRN AGPEKHQAVAARVQADRAIF+NCRFEGYQDTLYAQA
Sbjct: 361 TFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQA 420

Query: 421 HRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHETTGIVLQ 480
           HRQFYRSCLITGTIDFIFGDAAAIFQNCN+M+RKPL+NQQNIVTAQGRTD+HETTGIVLQ
Sbjct: 421 HRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGRTDKHETTGIVLQ 480

Query: 481 NCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDFALSTLF 540
           NC+I PDKTLE VKSQFKSYLGRPWKEFSRT+VMES IED+IHPDGW+ W+GDFAL TL+
Sbjct: 481 NCKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWMAWQGDFALKTLY 540

Query: 541 YAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVDWIESKSVPFHL 577
           YAEFNN+G GA+T+ARV+WPGYKVIDK+EA KFTIG+FLE+DWIES S P H+
Sbjct: 541 YAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHV 592

BLAST of Sgr019931 vs. NCBI nr
Match: KAG6596157.1 (putative pectinesterase/pectinesterase inhibitor 45, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 849.0 bits (2192), Expect = 2.5e-242
Identity = 430/598 (71.91%), Postives = 496/598 (82.94%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV--------------- 60
           MVFQDFDQISERRRAER RKFR RV IAVVSTF++L+LV AAV V               
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60

Query: 61  -SSKSGTANAKQPQTPATKEVSRVEKLISMICNSTDYKGKCESTLKDGVQSNPQSSDPKD 120
             S     ++K   T   K+VSR +K+I+MICNSTDYKGKCESTLK+GV+++P SSDPKD
Sbjct: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKGKCESTLKEGVETHPDSSDPKD 120

Query: 121 LLKVAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELEKSVTQVSNNAD 180
           L+K+AI+A   EV +A+KK +A NF  PEEK AFEDCK+L E+A+EEL++SV+++S   D
Sbjct: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRK-D 180

Query: 181 LKKLTSR-TPDLNNWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELTSNCLAMISQV 240
           L KLT + TPDLNNWLSAVMSY  TCVDGFPEGK K++ME  LKA KELTSN LAMISQV
Sbjct: 181 LGKLTEKTTPDLNNWLSAVMSYHATCVDGFPEGKSKTEMEATLKAGKELTSNSLAMISQV 240

Query: 241 ASFFTSFEMPEGAS----RHLLAHDGFPTWMTHEERRMLK-GAPADKPKPNVVVAKDGSG 300
           A+FF++FE  +G      R L+A DG P+WMT  ERRMLK  A  DKP PNVVVAKDGSG
Sbjct: 241 ATFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSG 300

Query: 301 DFKTISQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMITGNKNF 360
           +FKTI++ALAAMP KYEGRYVIYVKEGIYEETVVITKKM NVTMYGDGSQKSMI+GNKN 
Sbjct: 301 NFKTINEALAAMPAKYEGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNS 360

Query: 361 VDGVRTFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDT 420
            DGVR FQTA+F ALGDG +A+GIGFRNTAGPEK QAVAARVQ DRAIF+NCRFEG+QDT
Sbjct: 361 ADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDT 420

Query: 421 LYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHETT 480
           LYAQAHRQFYRSCLITGT+D IFGDAAAIFQNCN M++KPL+ Q N+VTAQGR+DRHETT
Sbjct: 421 LYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETT 480

Query: 481 GIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDFA 540
             VLQNC+I PDKTLE VK+QFKSYLGRP  E+SRT+VMES IEDIIHPDGW+PW+GDFA
Sbjct: 481 ATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFA 540

Query: 541 LSTLFYAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVDWIESKSVPFHL 577
           L TL+YAEFNNKG GA+T+ARV+WPG+KVIDKEEA KFTIG+FL+VDWI+S SV  H+
Sbjct: 541 LKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHV 597

BLAST of Sgr019931 vs. NCBI nr
Match: XP_023530461.1 (putative pectinesterase/pectinesterase inhibitor 45 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 848.6 bits (2191), Expect = 3.3e-242
Identity = 439/599 (73.29%), Postives = 493/599 (82.30%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV------SSKSGTAN- 60
           MVFQDFDQISERRR ER RKFR+RV I VV   V++LL+ AAV +      SS S T+N 
Sbjct: 1   MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIGAAVFIQVSPAPSSSSKTSNK 60

Query: 61  ----AKQPQTPA-----TKEVSRVEKLISMICNSTDYKGKCESTLKDGVQSNPQSSDPKD 120
                K P++ A     +K+ S  EK+I MICNSTDYK KCES LKDG+++NP+SSDPK+
Sbjct: 61  DGKSKKHPKSNADTDTESKDASSAEKMIVMICNSTDYKEKCESILKDGLKANPKSSDPKE 120

Query: 121 LLKVAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELEKSVTQVSNNAD 180
           L+K+AI+AA  EVK+A+KK T   F  PEEK AFEDCKVL EDA+EELE S+T VS    
Sbjct: 121 LIKLAITAAAAEVKSALKKATNLKFATPEEKGAFEDCKVLMEDAVEELEMSMTAVSKKNQ 180

Query: 181 LKKLTSRTPDLNNWLSAVMSYQETCVDGFPEG-KLKSDMEKALKAAKELTSNCLAMISQV 240
            K     TPDLNNWLSAVMSY ETCVDGFP+G K+K +MEK  K+ KELTSN LAMISQV
Sbjct: 181 GKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKHEMEKIGKSGKELTSNSLAMISQV 240

Query: 241 ASFFTSFEMP---EGAS--RHLLAHDGFPTWMTHEERRMLKGAPA-DKPKPNVVVAKDGS 300
           A+FF+ FE     EGA+  R L+  D  P+WM   ERRMLK A A DKP PNVVVA+DGS
Sbjct: 241 ATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKSATAGDKPTPNVVVAQDGS 300

Query: 301 GDFKTISQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMITGNKN 360
           G+FKTI++ALAAMP KY+GRYVIY+KEGIYEE VVITKKMVNVTMYGDGSQKSMI+GNKN
Sbjct: 301 GNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSMISGNKN 360

Query: 361 FVDGVRTFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQD 420
           F DGVRTFQTA+F ALGDGF AQ IGFRNTAGPEKHQAVAARVQADRAIFLNCRF+GYQD
Sbjct: 361 FADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFDGYQD 420

Query: 421 TLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHET 480
           TLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCN+MV+KPL+ QQNIVTAQGR+DRHET
Sbjct: 421 TLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMVKKPLDKQQNIVTAQGRSDRHET 480

Query: 481 TGIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDF 540
           T IVLQ C+I PDKTLE VKSQFKSYLGRPWKE+SRT+VMESTIEDIIHPDGWL WEGDF
Sbjct: 481 TAIVLQRCKILPDKTLEAVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWLAWEGDF 540

Query: 541 ALSTLFYAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVDWIESKSVPFHL 577
           AL TL+YAEFNN G GA+T  RV+WPG+KVIDKEEA KFTIGSFLEVDWI+S S P H+
Sbjct: 541 ALKTLYYAEFNNDGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWIQSTSAPVHV 599

BLAST of Sgr019931 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 2.6e-173
Identity = 318/611 (52.05%), Postives = 421/611 (68.90%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVV---------------- 60
           M FQDFD+I ER  AER+RKFRKR+ + VVS    +L+VAAA++                
Sbjct: 1   MAFQDFDKIQERVNAERKRKFRKRIILGVVS----VLVVAAAIIGGAFAYVTYENKTQEQ 60

Query: 61  ----------VSSKSGTANAKQPQTPA-TKEVSRVEKLISMICNSTDYKGKCESTLKDGV 120
                       +KS + + K P + A T +  +V+K+I  +CNST YK  C++TLK+  
Sbjct: 61  GKTTNNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNET 120

Query: 121 QSNPQSSDPKDLLKVAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELE 180
           + +   +DP+ LLK AI A  D++    K+  +      ++K A   CK+L ++A EEL 
Sbjct: 121 KKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELG 180

Query: 181 KSVTQVSNNADLKKLTSRTPDLNNWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELT 240
            S+ ++ N++++       PDL++WLSAVMSYQETCVDGF EGKLK+++ K   +++ LT
Sbjct: 181 TSMKRI-NDSEVNNFAKIVPDLDSWLSAVMSYQETCVDGFEEGKLKTEIRKNFNSSQVLT 240

Query: 241 SNCLAMISQVASFFTSFEMPEGASRHLL-------AHDGFPTWMTHEERRMLKGAPADKP 300
           SN LAMI  +  + +S  +P+  +R LL         D   +W++++ERRMLK       
Sbjct: 241 SNSLAMIKSLDGYLSS--VPKVKTRLLLEARSSAKETDHITSWLSNKERRMLKAVDVKAL 300

Query: 301 KPNVVVAKDGSGDFKTISQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDG 360
           KPN  VAKDGSG+F TI+ AL AMP KY+GRY IY+K GIY+E+V+I KK  NVTM GDG
Sbjct: 301 KPNATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDG 360

Query: 361 SQKSMITGNKNFVDGVRTFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAI 420
           SQK+++TGNK+    +RTF TATF A G+GFMAQ +GFRNTAGPE HQAVA RVQ+DR++
Sbjct: 361 SQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSV 420

Query: 421 FLNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIV 480
           FLNCRFEGYQDTLYA  HRQ+YRSC+I GT+DFIFGDAAAIFQNC+I +RK L  Q+N V
Sbjct: 421 FLNCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTV 480

Query: 481 TAQGRTDRHETTGIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIH 540
           TAQGR D+ +TTG V+ NC + P++ L+PVK+QFKSYLGRPWK  SRTVVMESTIED+I 
Sbjct: 481 TAQGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVID 540

Query: 541 PDGWLPW-EGDFALSTLFYAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVD 577
           P GWL W E DFA+ TL YAE+ N G    T ARV+WPG++V++KEEA KFT+G FL+ +
Sbjct: 541 PVGWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE 600

BLAST of Sgr019931 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 592.4 bits (1526), Expect = 5.6e-168
Identity = 310/616 (50.32%), Postives = 406/616 (65.91%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV--------------- 60
           M FQDFD+I ER  A R+RKFRKR+ +  VS  VV+  +                     
Sbjct: 1   MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60

Query: 61  ----SSKSGTAN---------AKQPQTPATK---------EVSRVEKLISMICNSTDYKG 120
                +KSG+ N         +  P TP+ K         +  + +K+I  +C+ST Y  
Sbjct: 61  QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120

Query: 121 KCESTLKDGVQSNPQSSDPKDLLKVAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKV 180
            CE TLK+         +P   LK AI A  +++   ++K  +      ++K A E CK+
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180

Query: 181 LFEDALEELEKSVTQVSNNADLKKLTSRTPDLNNWLSAVMSYQETCVDGFPEGKLKSDME 240
           L EDA EE   S+ ++ N  ++       PDL +WLSAVMSYQETC+DGF EG LKS+++
Sbjct: 181 LVEDAKEETVASLNKI-NVTEVNSFEKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVK 240

Query: 241 KALKAAKELTSNCLAMISQVASFFTSFEMP--EGASRHLLAHDGFPTWMTHEERRMLKGA 300
            ++ +++ LTSN LA+I      FT    P  +   RHLL  D  P+W+++++RRML+  
Sbjct: 241 TSVNSSQVLTSNSLALIKT----FTENLSPVMKVVERHLL--DDIPSWVSNDDRRMLRAV 300

Query: 301 PADKPKPNVVVAKDGSGDFKTISQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVT 360
                KPN  VAKDGSGDF TI+ AL AMP KYEGRY+IYVK+GIY+E V + KK  N+T
Sbjct: 301 DVKALKPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLT 360

Query: 361 MYGDGSQKSMITGNKNFVDGVRTFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQ 420
           M GDGSQK+++TGNK+    +RTF TATF A G+GFMAQ +GFRNTAGPE HQAVA RVQ
Sbjct: 361 MVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQ 420

Query: 421 ADRAIFLNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLEN 480
           +DR+IFLNCRFEGYQDTLYA  HRQ+YRSC+I GTIDFIFGDAAAIFQNCNI +RK L  
Sbjct: 421 SDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPG 480

Query: 481 QQNIVTAQGRTDRHETTGIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTI 540
           Q+N VTAQGR D+ +TTG V+ NC+I  ++ L+PVK ++KSYLGRPWK +SRT++MES I
Sbjct: 481 QKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKI 540

Query: 541 EDIIHPDGWLPW-EGDFALSTLFYAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGS 577
           E++I P GWL W E DFA+ TL+YAE+NNKG+   T +RV+WPG+KVI+KEEA  +T+G 
Sbjct: 541 ENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVGP 600

BLAST of Sgr019931 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 7.7e-133
Identity = 251/566 (44.35%), Postives = 362/566 (63.96%), Query Frame = 0

Query: 16  ERERKFRKRVTIAVVSTFVVLLLVAAAVVVSSKSGTANAKQPQTPATKEVSRVEKLISMI 75
           + + K +KR  I  +S+ +++ +V A  +  S + + NA        +E++   K I  +
Sbjct: 7   DEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNA------GDEEITTSVKAIKDV 66

Query: 76  CNSTDYKGKCESTLKDGVQSNPQSSDPKDLLKVAISAAVDEVKAAVKKT-TAFNFGK-PE 135
           C  TDYK  CE TL+   +    +SDP +L+K A +A + ++    KK+ T     K P 
Sbjct: 67  CAPTDYKETCEDTLRKDAK---DTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 126

Query: 136 EKAAFEDCKVLFEDALEELEKSVTQVSNNADLKKLTSRTPDLNNWLSAVMSYQETCVDGF 195
            K A + CK L + A+ EL KS  ++    +  K+      L  WLSA +S+++TC+DGF
Sbjct: 127 AKMALDQCKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGF 186

Query: 196 --PEGKLKSDMEKALKAAKELTSNCLAMISQVASFFTSFEMPEGASRHLLAHDGFPTWMT 255
              +G     ++KALK A +LT N LAM+++++++    ++PE  SR LL+ + FP+WM 
Sbjct: 187 QGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQE-FPSWMD 246

Query: 256 HEERRMLKGAPADKPKPNVVVAKDGSGDFKTISQALAAMPPKYEGRYVIYVKEGIYEETV 315
              RR+L  AP  + KP++VVA+DGSG +KTI++AL  +P K    +V+++KEGIY+E V
Sbjct: 247 ARARRLL-NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYV 306

Query: 316 VITKKMVNVTMYGDGSQKSMITGNKNFVDGVRTFQTATFAALGDGFMAQGIGFRNTAGPE 375
            + + M ++   GDG  K++I+G+K++ DG+ T++TAT A +GD F+A+ I F NTAG  
Sbjct: 307 QVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAI 366

Query: 376 KHQAVAARVQADRAIFLNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNC 435
           KHQAVA RV AD +IF NC+F+GYQDTLYA +HRQFYR C I+GTIDF+FGDAAA+FQNC
Sbjct: 367 KHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNC 426

Query: 436 NIMVRKPLENQQNIVTAQGRTDRHETTGIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKEF 495
            ++VRKPL NQ   +TA GR D  E+TG VLQ C I  +     VK Q K+YLGRPWKE+
Sbjct: 427 TLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEY 486

Query: 496 SRTVVMESTIEDIIHPDGWLPWEGDFALSTLFYAEFNNKGAGARTNARVRWPGYKVIDKE 555
           SRT++M + I D + P+GW PW G+F L+TLFY+E  N G GA    RV WPG K +  E
Sbjct: 487 SRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDE 546

Query: 556 EATKFTIGSFLEVD-WIESKSVPFHL 577
           E  KFT   +++ D WI  K VP+ L
Sbjct: 547 EILKFTPAQYIQGDAWIPGKGVPYIL 560

BLAST of Sgr019931 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 8.5e-132
Identity = 248/572 (43.36%), Postives = 359/572 (62.76%), Query Frame = 0

Query: 18  ERKFRKRVTIAVVSTFVVLLLVAAAVVVSSKSGTANAKQPQTPATKEVSRVEKLISMICN 77
           E K +K +   V++  +VL++VA  +  S      N    +     ++      +  +C 
Sbjct: 6   ELKKKKCIIAGVITALLVLMVVAVGITTS-----RNTSHSEKIVPVQIKTATTAVEAVCA 65

Query: 78  STDYKGKCESTLKDGVQSNPQSSDPKDLLKVAISAAVDEVKAAVKKT----TAFNFGKPE 137
            TDYK  C ++L   ++++P S+ P DL+K+  +  +  ++ ++KK     TA      +
Sbjct: 66  PTDYKETCVNSL---MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 125

Query: 138 EKAAFEDCKVLFEDALEELEKSVTQVSNNADLKKLTSRTPDLNNWLSAVMSYQETCVDGF 197
            K A E C+ L  DA ++L+K +    +   + ++     DL  WLS  ++YQ+TC+D F
Sbjct: 126 TKGALELCEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTF 185

Query: 198 PE--GKLKSDMEKALKAAKELTSNCLAMISQVASFFTSF-------EMPEGASRHLLAHD 257
            E   KL  DM+K  K ++ELTSN LAMI+ +++    F       ++ + A + L A D
Sbjct: 186 EETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED 245

Query: 258 GFPTWMTHEERRMLKGAPADKPKPNVVVAKDGSGDFKTISQALAAMPPKYEGRYVIYVKE 317
           G P+W+    RR++  A     K NVVVA DGSG +KTI++AL A+P   +  +VIY+K+
Sbjct: 246 GIPSWVGPNTRRLM--ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQ 305

Query: 318 GIYEETVVITKKMVNVTMYGDGSQKSMITGNKNFVDG-VRTFQTATFAALGDGFMAQGIG 377
           G+Y E V +TKKM +VT  GDG  K+ ITG+ N+  G V+T+ TAT A  GD F A+ IG
Sbjct: 306 GVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIG 365

Query: 378 FRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGD 437
           F NTAGPE HQAVA RV AD A+F NC+ +GYQDTLY  +HRQF+R C ++GT+DFIFGD
Sbjct: 366 FENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGD 425

Query: 438 AAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHETTGIVLQNCRIQPDKTLEPVKSQFKSY 497
              + QNCNI+VRKP+++Q  ++TAQGR+D+ E+TG+VLQNC I  +    PVKS  K+Y
Sbjct: 426 GIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAY 485

Query: 498 LGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDFALSTLFYAEFNNKGAGARTNARVRWP 557
           LGRPWKEFSRT++M +TI+D+I P GWLPW GDFAL+TL+YAE+ N G G+    RV+WP
Sbjct: 486 LGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWP 545

Query: 558 GYKVIDKEEATKFTIGSFLEVD-WIESKSVPF 575
           G K +  ++A +FT   FL  + WI    VP+
Sbjct: 546 GIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of Sgr019931 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 463.4 bits (1191), Expect = 3.9e-129
Identity = 244/565 (43.19%), Postives = 355/565 (62.83%), Query Frame = 0

Query: 16  ERERKFRKRVTIAVVSTFVVLLLVAAAVVVSSKSGTANAKQPQTPATKEVSRVEKLISMI 75
           +  ++ R+ + I + S  ++ ++VA  V VS      ++K        EV+   K +  +
Sbjct: 7   DESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSK-----GKAEVNASVKAVKDV 66

Query: 76  CNSTDYKGKCESTLKDGVQSNPQSSDPKDLLKVAISAAVDEVKAAVKKT-TAFNFGKPEE 135
           C  TDY+  CE TL   +++   ++DP +L+K A +  + ++  A KK+ T     K   
Sbjct: 67  CAPTDYRKTCEDTL---IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 126

Query: 136 -KAAFEDCKVLFEDALEELEKSVTQVSNNADLKKLTSRTPDLNNWLSAVMSYQETCVDGF 195
            + A + CK L + AL+EL  S  ++    +   L     +L  WLSA +S++ETC++GF
Sbjct: 127 TRMALDQCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGF 186

Query: 196 --PEGKLKSDMEKALKAAKELTSNCLAMISQVASFFTSFEMPEGASRHLLAHDGFPTWMT 255
              +G     M+KALK A ELT N LA+IS++++F    ++P   SR LLA +GFP+W+ 
Sbjct: 187 QGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA-EGFPSWVD 246

Query: 256 HEERRMLKGAPA-DKPKPNVVVAKDGSGDFKTISQALAAMPPKYEGRYVIYVKEGIYEET 315
              R++L+ A A    KP++VVA+DGSG +KTI++AL  +P K    +V+++K G+Y+E 
Sbjct: 247 QRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEY 306

Query: 316 VVITKKMVNVTMYGDGSQKSMITGNKNFVDGVRTFQTATFAALGDGFMAQGIGFRNTAGP 375
           V + K M ++   GDG  K++I+GNKN+ DG+ T++TAT A +G+ F+A+ IGF NTAG 
Sbjct: 307 VQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGA 366

Query: 376 EKHQAVAARVQADRAIFLNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQN 435
            KHQAVA RVQ+D +IF NCRF+GYQDTLY  +HRQF+R C I+GTIDF+FGDAAA+FQN
Sbjct: 367 IKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQN 426

Query: 436 CNIMVRKPLENQQNIVTAQGRTDRHETTGIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKE 495
           C ++VRKPL NQ   +TA GR D  E+TG V Q C I  +     VK   K+YLGRPWKE
Sbjct: 427 CTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKE 486

Query: 496 FSRTVVMESTIEDIIHPDGWLPWEGDFALSTLFYAEFNNKGAGARTNARVRWPGYKVIDK 555
           +SRT++M + I D + P GW PW GDF L TLFY+E  N G G+    RV W G K + +
Sbjct: 487 YSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSE 546

Query: 556 EEATKFTIGSFLE-VDWIESKSVPF 575
           E+  KFT   +++  DWI  K VP+
Sbjct: 547 EDILKFTPAQYIQGDDWIPGKGVPY 561

BLAST of Sgr019931 vs. ExPASy TrEMBL
Match: A0A0A0KX33 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G630740 PE=3 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 4.6e-266
Identity = 467/592 (78.89%), Postives = 525/592 (88.68%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV---SSKSGT------ 60
           MVFQDFDQISERRRAER RKFRKRV IAVVS  V+LL+VAAAV V   SSKS T      
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQVSSSKSTTKNDDSG 60

Query: 61  ---ANAKQPQTPATKEVSRVEKLISMICNSTDYKGKCESTLKDGVQSNPQSSDPKDLLKV 120
               N+KQP  P TK+VSRVEK+I+MICNSTDYKGKCESTLKDGV ++P SSDPKDL+K+
Sbjct: 61  SSSGNSKQPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGVHTDPNSSDPKDLIKL 120

Query: 121 AISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELEKSVTQVSNNADLKKL 180
           AISAA  EVK+AVKK + FNF  PEEK AFEDCKVL EDA+EELE S+++V N  ++ KL
Sbjct: 121 AISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAIEELEMSMSEV-NKKNMGKL 180

Query: 181 TSR-TPDLNNWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELTSNCLAMISQVASFF 240
           T++ TP+LNNWLSAVMSY ETCVDGFPEGK+KSD+EK +KA KELTSN LAMISQVASFF
Sbjct: 181 TAKTTPNLNNWLSAVMSYHETCVDGFPEGKMKSDIEKVVKAGKELTSNSLAMISQVASFF 240

Query: 241 TSFEMPEGAS--RHLLAHDGFPTWMTHEERRMLKGAPA-DKPKPNVVVAKDGSGDFKTIS 300
           ++FEMPEGA+  R L+  +G PTWM   +RRMLKGA A +KPKPNVVVAKDGSG+FKTI+
Sbjct: 241 STFEMPEGAASRRRLMTTNGVPTWMDRNQRRMLKGAAAGEKPKPNVVVAKDGSGEFKTIN 300

Query: 301 QALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMITGNKNFVDGVRT 360
           +ALAAMP KY+GRYVIYVKEGIY+ETVVITKKMVNVTMYGDGSQKSMI+G+KNFVDGVRT
Sbjct: 301 EALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVRT 360

Query: 361 FQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYAQAH 420
           FQTATF ALG+GF+ Q IGFRN AGPEKHQAVAARVQADRAIF+NCRFEGYQDTLY QAH
Sbjct: 361 FQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYTQAH 420

Query: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHETTGIVLQN 480
           RQFYRSCLITGTIDFIFGDAAAIFQNCN+M+RKPL+NQQN+VTAQGRTD+HETTGIVLQN
Sbjct: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNMVTAQGRTDKHETTGIVLQN 480

Query: 481 CRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDFALSTLFY 540
           C+I PDKTLEPVKSQFKSYLGRPWKEFSRT+VMESTIED+IHPDGW+ WEGDFAL TL+Y
Sbjct: 481 CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDVIHPDGWMAWEGDFALKTLYY 540

Query: 541 AEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVDWIESKSVPFHL 577
           AEFNNKG GA+T+ARV+WPGYKVIDK+EA KFTIG+FLE+DWIES S P H+
Sbjct: 541 AEFNNKGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHV 591

BLAST of Sgr019931 vs. ExPASy TrEMBL
Match: A0A5D3BLU5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2460G00020 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.3e-265
Identity = 465/593 (78.41%), Postives = 523/593 (88.20%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV-------------SS 60
           MVFQDFDQISERRRAER RKFRKRVTIAVVS  V+LL+VAAAV V              S
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVSSSSKSTTKNGDS 60

Query: 61  KSGTANAKQPQTPATKEVSRVEKLISMICNSTDYKGKCESTLKDGVQSNPQSSDPKDLLK 120
            S T N+K+P  P TK+VSRVEK+I+MICNSTDYKGKCESTLKDG+ ++P SSDPKDL+K
Sbjct: 61  GSSTGNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPNSSDPKDLIK 120

Query: 121 VAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELEKSVTQVSNNADLKK 180
           +AISAA  EVK+AVKK + FNF  PEEK AFEDCKVL EDA+EELE S+++VS   ++ K
Sbjct: 121 LAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKK-NMGK 180

Query: 181 LTSR-TPDLNNWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELTSNCLAMISQVASF 240
           LT++ TPDLNNWLSAVMSY ETCVDGFPEGK+KS+MEK +KA KELTSN LAMISQVASF
Sbjct: 181 LTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMKSEMEKVVKAGKELTSNSLAMISQVASF 240

Query: 241 FTSFEMPEGAS--RHLLAHDGFPTWMTHEERRMLKGAPA-DKPKPNVVVAKDGSGDFKTI 300
           F++FEMPEGA+  R L+A  G PTWM   ERRMLKGA A +KPKPNVVVAKDGSG+FKTI
Sbjct: 241 FSTFEMPEGAASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVVVAKDGSGEFKTI 300

Query: 301 SQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMITGNKNFVDGVR 360
           ++ALAAMP KY+GRYVIYVKEGIY+ETVVITKKMVNVTMYGDGSQKSMI+G+KNFVDGVR
Sbjct: 301 NEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVR 360

Query: 361 TFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYAQA 420
           TFQTATF ALG+GF+ Q IGFRN AGPEKHQAVAARVQADRAIF+NCRFEGYQDTLYAQA
Sbjct: 361 TFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQA 420

Query: 421 HRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHETTGIVLQ 480
           HRQFYRSCLITGTIDFIFGDAAAIFQNCN+M+RKPL+NQQNIVTAQGRTD+HETTGIVLQ
Sbjct: 421 HRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGRTDKHETTGIVLQ 480

Query: 481 NCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDFALSTLF 540
           NC+I PDKTLE VKSQFKSYLGRPWKEFSRT+VMES IED+IHPDGW+ W+GDFAL TL+
Sbjct: 481 NCKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWMAWQGDFALKTLY 540

Query: 541 YAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVDWIESKSVPFHL 577
           YAEFNN+G GA+T+ARV+WPGYKVIDK+EA KFTIG+FLE+DWIES S P H+
Sbjct: 541 YAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHV 592

BLAST of Sgr019931 vs. ExPASy TrEMBL
Match: A0A1S3CCA7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499351 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.3e-265
Identity = 465/593 (78.41%), Postives = 523/593 (88.20%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV-------------SS 60
           MVFQDFDQISERRRAER RKFRKRVTIAVVS  V+LL+VAAAV V              S
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVSSSSKSTTKNGDS 60

Query: 61  KSGTANAKQPQTPATKEVSRVEKLISMICNSTDYKGKCESTLKDGVQSNPQSSDPKDLLK 120
            S T N+K+P  P TK+VSRVEK+I+MICNSTDYKGKCESTLKDG+ ++P SSDPKDL+K
Sbjct: 61  GSSTGNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPNSSDPKDLIK 120

Query: 121 VAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELEKSVTQVSNNADLKK 180
           +AISAA  EVK+AVKK + FNF  PEEK AFEDCKVL EDA+EELE S+++VS   ++ K
Sbjct: 121 LAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKK-NMGK 180

Query: 181 LTSR-TPDLNNWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELTSNCLAMISQVASF 240
           LT++ TPDLNNWLSAVMSY ETCVDGFPEGK+KS+MEK +KA KELTSN LAMISQVASF
Sbjct: 181 LTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMKSEMEKVVKAGKELTSNSLAMISQVASF 240

Query: 241 FTSFEMPEGAS--RHLLAHDGFPTWMTHEERRMLKGAPA-DKPKPNVVVAKDGSGDFKTI 300
           F++FEMPEGA+  R L+A  G PTWM   ERRMLKGA A +KPKPNVVVAKDGSG+FKTI
Sbjct: 241 FSTFEMPEGAASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVVVAKDGSGEFKTI 300

Query: 301 SQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMITGNKNFVDGVR 360
           ++ALAAMP KY+GRYVIYVKEGIY+ETVVITKKMVNVTMYGDGSQKSMI+G+KNFVDGVR
Sbjct: 301 NEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVR 360

Query: 361 TFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYAQA 420
           TFQTATF ALG+GF+ Q IGFRN AGPEKHQAVAARVQADRAIF+NCRFEGYQDTLYAQA
Sbjct: 361 TFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQA 420

Query: 421 HRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHETTGIVLQ 480
           HRQFYRSCLITGTIDFIFGDAAAIFQNCN+M+RKPL+NQQNIVTAQGRTD+HETTGIVLQ
Sbjct: 421 HRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGRTDKHETTGIVLQ 480

Query: 481 NCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDFALSTLF 540
           NC+I PDKTLE VKSQFKSYLGRPWKEFSRT+VMES IED+IHPDGW+ W+GDFAL TL+
Sbjct: 481 NCKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWMAWQGDFALKTLY 540

Query: 541 YAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVDWIESKSVPFHL 577
           YAEFNN+G GA+T+ARV+WPGYKVIDK+EA KFTIG+FLE+DWIES S P H+
Sbjct: 541 YAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHV 592

BLAST of Sgr019931 vs. ExPASy TrEMBL
Match: A0A6J1I3R6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470280 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 2.1e-242
Identity = 430/601 (71.55%), Postives = 496/601 (82.53%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV--------------- 60
           MVFQDFDQISERRRAER RKFR RV IAVVSTF++L+ V AAV V               
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVFVVAAVFVQVSPPTKSTTRDGAG 60

Query: 61  ----SSKSGTANAKQPQTPATKEVSRVEKLISMICNSTDYKGKCESTLKDGVQSNPQSSD 120
               SS     ++K   T   K+VSR +K+I+MICNSTDYKGKCESTLK GV+++P SSD
Sbjct: 61  AGSSSSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKGKCESTLKQGVETHPDSSD 120

Query: 121 PKDLLKVAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELEKSVTQVSN 180
           PKDL+K+AI+A   EV +A+KK +AFNF  PEEK AFEDCK+L E+A+EEL++SV+++S 
Sbjct: 121 PKDLIKLAIAAVSTEVNSALKKASAFNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSR 180

Query: 181 NADLKKLTSR-TPDLNNWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELTSNCLAMI 240
             DL KLT + TPDLNNWLSAVMSY  TCVDGFPEGK K+ ME  LKA KELTSN LAMI
Sbjct: 181 K-DLGKLTDKTTPDLNNWLSAVMSYHATCVDGFPEGKSKTVMEATLKAGKELTSNSLAMI 240

Query: 241 SQVASFFTSFEMPEGAS----RHLLAHDGFPTWMTHEERRMLKG-APADKPKPNVVVAKD 300
           SQVA+FF++FE  +G      R L+A DG P+WMT  ERRMLKG A  DKP PNVVVAKD
Sbjct: 241 SQVATFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSNERRMLKGDAADDKPTPNVVVAKD 300

Query: 301 GSGDFKTISQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMITGN 360
           GSG+FKTI++ALAAMP KY+GRYVIYVKEGIYEETVVITKKM NVTMYGDGSQKSMI+GN
Sbjct: 301 GSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGN 360

Query: 361 KNFVDGVRTFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGY 420
           KN  DGVR FQTA+F ALGDG +A+GIGFRNTAGPEK QAVAARVQ DRAIF+NCRFEG+
Sbjct: 361 KNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGF 420

Query: 421 QDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIVTAQGRTDRH 480
           QDTLYAQAHRQFYRSCLITGT+D IFGDAAAIFQNCN M++KPL+ Q N+VTAQGR+DRH
Sbjct: 421 QDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRH 480

Query: 481 ETTGIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIHPDGWLPWEG 540
           ETT  VLQNC+I PDKTLE VK+QFKSYLGRP  E+SRT+VMES IEDIIHPDGW+PW+G
Sbjct: 481 ETTATVLQNCKILPDKTLEAVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDG 540

Query: 541 DFALSTLFYAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVDWIESKSVPFH 577
           DFAL TL+YAEF+NKG GA+T+ARV+WPG+KVIDKEEA KFTIG+FL+VDWI+S SV  H
Sbjct: 541 DFALKTLYYAEFSNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVH 600

BLAST of Sgr019931 vs. ExPASy TrEMBL
Match: A0A2N9H3N6 (Pectinesterase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34534 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 6.1e-242
Identity = 416/579 (71.85%), Postives = 489/579 (84.46%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAA--VVVSSKSGTANAK--Q 60
           M FQDFD ISERRRAE++RK RK++TIA V  FV+ L++AAA  VV+++KSG  + K  Q
Sbjct: 1   MAFQDFDHISERRRAEKQRKLRKKITIATVLVFVLALIIAAAVFVVLTNKSGHGSTKPAQ 60

Query: 61  PQTPATKEVSRVEKLISMICNSTDYKGKCESTLKDGVQSNPQSSDPKDLLKVAISAAVDE 120
           PQ  A K++S   K+I MICNST+YK KCE+TL   V++NP  S PKDLLK AISAA DE
Sbjct: 61  PQQEA-KQISHSVKIIKMICNSTEYKDKCENTLNKAVKANPNLSQPKDLLKSAISAAADE 120

Query: 121 VKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELEKSVTQVSNNADLKKLTSRTPDLN 180
              A+KKTTAF F  P+EKAAFEDCKVL +DA+EEL  SV+QV NN +L+KL S TPDLN
Sbjct: 121 FTKALKKTTAFKFNTPKEKAAFEDCKVLMQDAMEELADSVSQVGNN-NLRKLPSTTPDLN 180

Query: 181 NWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELTSNCLAMISQVASFFTSFEMPEGA 240
           NWLSAVMSYQ+TC+DGFP+GKLK +ME ALKAAKE TSN LA++S+V SF ++F+   G 
Sbjct: 181 NWLSAVMSYQQTCIDGFPQGKLKDNMENALKAAKEFTSNSLAIVSEVTSFLSTFQNTPGL 240

Query: 241 SRHLLAHDGFPTWMTHEERRMLKGAPADKPKPNVVVAKDGSGDFKTISQALAAMPPKYEG 300
           SRHLL  DG PTWM+HE+RRMLK    DKP PNV VAKDGSGD+KTISQALAAMP KY+G
Sbjct: 241 SRHLLEKDGLPTWMSHEDRRMLKAKNIDKPTPNVTVAKDGSGDYKTISQALAAMPEKYKG 300

Query: 301 RYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMITGNKNFVDGVRTFQTATFAALGDG 360
           RYVIYVKEGIY+ETV +TKKMVNVTMYGDGSQKS+ITG+KNFVDGVRTFQTA+F ALG+G
Sbjct: 301 RYVIYVKEGIYDETVTVTKKMVNVTMYGDGSQKSIITGSKNFVDGVRTFQTASFVALGEG 360

Query: 361 FMAQGIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYAQAHRQFYRSCLITGT 420
           FMA+ +GFRNTAGPEKHQAVAARVQADR+IFLNCRFE YQDTLYAQ HRQFYRSC+I GT
Sbjct: 361 FMAKAMGFRNTAGPEKHQAVAARVQADRSIFLNCRFEAYQDTLYAQTHRQFYRSCVIAGT 420

Query: 421 IDFIFGDAAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHETTGIVLQNCRIQPDKTLEPV 480
           +DFIFGDAAA+FQNC I+VRKPL+NQQNIVTAQGR D+ ETTGIVLQNCRI  DK L PV
Sbjct: 421 VDFIFGDAAAVFQNCMIVVRKPLDNQQNIVTAQGRIDKRETTGIVLQNCRILADKKLVPV 480

Query: 481 KSQFKSYLGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDFALSTLFYAEFNNKGAGART 540
           KS+ +SYLGRPWKE+SRT++MESTIED+IHPDGWLPWEGDFAL TL+YAE+ N+G GA+ 
Sbjct: 481 KSKIRSYLGRPWKEYSRTIIMESTIEDVIHPDGWLPWEGDFALKTLYYAEYKNEGPGAKV 540

Query: 541 NARVRWPGYKVIDKEEATKFTIGSFLEVDWIESKSVPFH 576
           +AR++WPGYK I  +EATK+T+G FL+ +WI +  VP H
Sbjct: 541 DARIKWPGYKKITAQEATKYTVGPFLQGNWINAIGVPVH 577

BLAST of Sgr019931 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 610.1 bits (1572), Expect = 1.8e-174
Identity = 318/611 (52.05%), Postives = 421/611 (68.90%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVV---------------- 60
           M FQDFD+I ER  AER+RKFRKR+ + VVS    +L+VAAA++                
Sbjct: 1   MAFQDFDKIQERVNAERKRKFRKRIILGVVS----VLVVAAAIIGGAFAYVTYENKTQEQ 60

Query: 61  ----------VSSKSGTANAKQPQTPA-TKEVSRVEKLISMICNSTDYKGKCESTLKDGV 120
                       +KS + + K P + A T +  +V+K+I  +CNST YK  C++TLK+  
Sbjct: 61  GKTTNNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNET 120

Query: 121 QSNPQSSDPKDLLKVAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKVLFEDALEELE 180
           + +   +DP+ LLK AI A  D++    K+  +      ++K A   CK+L ++A EEL 
Sbjct: 121 KKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELG 180

Query: 181 KSVTQVSNNADLKKLTSRTPDLNNWLSAVMSYQETCVDGFPEGKLKSDMEKALKAAKELT 240
            S+ ++ N++++       PDL++WLSAVMSYQETCVDGF EGKLK+++ K   +++ LT
Sbjct: 181 TSMKRI-NDSEVNNFAKIVPDLDSWLSAVMSYQETCVDGFEEGKLKTEIRKNFNSSQVLT 240

Query: 241 SNCLAMISQVASFFTSFEMPEGASRHLL-------AHDGFPTWMTHEERRMLKGAPADKP 300
           SN LAMI  +  + +S  +P+  +R LL         D   +W++++ERRMLK       
Sbjct: 241 SNSLAMIKSLDGYLSS--VPKVKTRLLLEARSSAKETDHITSWLSNKERRMLKAVDVKAL 300

Query: 301 KPNVVVAKDGSGDFKTISQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVTMYGDG 360
           KPN  VAKDGSG+F TI+ AL AMP KY+GRY IY+K GIY+E+V+I KK  NVTM GDG
Sbjct: 301 KPNATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDG 360

Query: 361 SQKSMITGNKNFVDGVRTFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQADRAI 420
           SQK+++TGNK+    +RTF TATF A G+GFMAQ +GFRNTAGPE HQAVA RVQ+DR++
Sbjct: 361 SQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSV 420

Query: 421 FLNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLENQQNIV 480
           FLNCRFEGYQDTLYA  HRQ+YRSC+I GT+DFIFGDAAAIFQNC+I +RK L  Q+N V
Sbjct: 421 FLNCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTV 480

Query: 481 TAQGRTDRHETTGIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTIEDIIH 540
           TAQGR D+ +TTG V+ NC + P++ L+PVK+QFKSYLGRPWK  SRTVVMESTIED+I 
Sbjct: 481 TAQGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVID 540

Query: 541 PDGWLPW-EGDFALSTLFYAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGSFLEVD 577
           P GWL W E DFA+ TL YAE+ N G    T ARV+WPG++V++KEEA KFT+G FL+ +
Sbjct: 541 PVGWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE 600

BLAST of Sgr019931 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 592.4 bits (1526), Expect = 4.0e-169
Identity = 310/616 (50.32%), Postives = 406/616 (65.91%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERERKFRKRVTIAVVSTFVVLLLVAAAVVV--------------- 60
           M FQDFD+I ER  A R+RKFRKR+ +  VS  VV+  +                     
Sbjct: 1   MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60

Query: 61  ----SSKSGTAN---------AKQPQTPATK---------EVSRVEKLISMICNSTDYKG 120
                +KSG+ N         +  P TP+ K         +  + +K+I  +C+ST Y  
Sbjct: 61  QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120

Query: 121 KCESTLKDGVQSNPQSSDPKDLLKVAISAAVDEVKAAVKKTTAFNFGKPEEKAAFEDCKV 180
            CE TLK+         +P   LK AI A  +++   ++K  +      ++K A E CK+
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180

Query: 181 LFEDALEELEKSVTQVSNNADLKKLTSRTPDLNNWLSAVMSYQETCVDGFPEGKLKSDME 240
           L EDA EE   S+ ++ N  ++       PDL +WLSAVMSYQETC+DGF EG LKS+++
Sbjct: 181 LVEDAKEETVASLNKI-NVTEVNSFEKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVK 240

Query: 241 KALKAAKELTSNCLAMISQVASFFTSFEMP--EGASRHLLAHDGFPTWMTHEERRMLKGA 300
            ++ +++ LTSN LA+I      FT    P  +   RHLL  D  P+W+++++RRML+  
Sbjct: 241 TSVNSSQVLTSNSLALIKT----FTENLSPVMKVVERHLL--DDIPSWVSNDDRRMLRAV 300

Query: 301 PADKPKPNVVVAKDGSGDFKTISQALAAMPPKYEGRYVIYVKEGIYEETVVITKKMVNVT 360
                KPN  VAKDGSGDF TI+ AL AMP KYEGRY+IYVK+GIY+E V + KK  N+T
Sbjct: 301 DVKALKPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLT 360

Query: 361 MYGDGSQKSMITGNKNFVDGVRTFQTATFAALGDGFMAQGIGFRNTAGPEKHQAVAARVQ 420
           M GDGSQK+++TGNK+    +RTF TATF A G+GFMAQ +GFRNTAGPE HQAVA RVQ
Sbjct: 361 MVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQ 420

Query: 421 ADRAIFLNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNIMVRKPLEN 480
           +DR+IFLNCRFEGYQDTLYA  HRQ+YRSC+I GTIDFIFGDAAAIFQNCNI +RK L  
Sbjct: 421 SDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPG 480

Query: 481 QQNIVTAQGRTDRHETTGIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKEFSRTVVMESTI 540
           Q+N VTAQGR D+ +TTG V+ NC+I  ++ L+PVK ++KSYLGRPWK +SRT++MES I
Sbjct: 481 QKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKI 540

Query: 541 EDIIHPDGWLPW-EGDFALSTLFYAEFNNKGAGARTNARVRWPGYKVIDKEEATKFTIGS 577
           E++I P GWL W E DFA+ TL+YAE+NNKG+   T +RV+WPG+KVI+KEEA  +T+G 
Sbjct: 541 ENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVGP 600

BLAST of Sgr019931 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 475.7 bits (1223), Expect = 5.4e-134
Identity = 251/566 (44.35%), Postives = 362/566 (63.96%), Query Frame = 0

Query: 16  ERERKFRKRVTIAVVSTFVVLLLVAAAVVVSSKSGTANAKQPQTPATKEVSRVEKLISMI 75
           + + K +KR  I  +S+ +++ +V A  +  S + + NA        +E++   K I  +
Sbjct: 7   DEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNA------GDEEITTSVKAIKDV 66

Query: 76  CNSTDYKGKCESTLKDGVQSNPQSSDPKDLLKVAISAAVDEVKAAVKKT-TAFNFGK-PE 135
           C  TDYK  CE TL+   +    +SDP +L+K A +A + ++    KK+ T     K P 
Sbjct: 67  CAPTDYKETCEDTLRKDAK---DTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 126

Query: 136 EKAAFEDCKVLFEDALEELEKSVTQVSNNADLKKLTSRTPDLNNWLSAVMSYQETCVDGF 195
            K A + CK L + A+ EL KS  ++    +  K+      L  WLSA +S+++TC+DGF
Sbjct: 127 AKMALDQCKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGF 186

Query: 196 --PEGKLKSDMEKALKAAKELTSNCLAMISQVASFFTSFEMPEGASRHLLAHDGFPTWMT 255
              +G     ++KALK A +LT N LAM+++++++    ++PE  SR LL+ + FP+WM 
Sbjct: 187 QGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQE-FPSWMD 246

Query: 256 HEERRMLKGAPADKPKPNVVVAKDGSGDFKTISQALAAMPPKYEGRYVIYVKEGIYEETV 315
              RR+L  AP  + KP++VVA+DGSG +KTI++AL  +P K    +V+++KEGIY+E V
Sbjct: 247 ARARRLL-NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYV 306

Query: 316 VITKKMVNVTMYGDGSQKSMITGNKNFVDGVRTFQTATFAALGDGFMAQGIGFRNTAGPE 375
            + + M ++   GDG  K++I+G+K++ DG+ T++TAT A +GD F+A+ I F NTAG  
Sbjct: 307 QVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAI 366

Query: 376 KHQAVAARVQADRAIFLNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNC 435
           KHQAVA RV AD +IF NC+F+GYQDTLYA +HRQFYR C I+GTIDF+FGDAAA+FQNC
Sbjct: 367 KHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNC 426

Query: 436 NIMVRKPLENQQNIVTAQGRTDRHETTGIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKEF 495
            ++VRKPL NQ   +TA GR D  E+TG VLQ C I  +     VK Q K+YLGRPWKE+
Sbjct: 427 TLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEY 486

Query: 496 SRTVVMESTIEDIIHPDGWLPWEGDFALSTLFYAEFNNKGAGARTNARVRWPGYKVIDKE 555
           SRT++M + I D + P+GW PW G+F L+TLFY+E  N G GA    RV WPG K +  E
Sbjct: 487 SRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDE 546

Query: 556 EATKFTIGSFLEVD-WIESKSVPFHL 577
           E  KFT   +++ D WI  K VP+ L
Sbjct: 547 EILKFTPAQYIQGDAWIPGKGVPYIL 560

BLAST of Sgr019931 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 472.2 bits (1214), Expect = 6.0e-133
Identity = 248/572 (43.36%), Postives = 359/572 (62.76%), Query Frame = 0

Query: 18  ERKFRKRVTIAVVSTFVVLLLVAAAVVVSSKSGTANAKQPQTPATKEVSRVEKLISMICN 77
           E K +K +   V++  +VL++VA  +  S      N    +     ++      +  +C 
Sbjct: 6   ELKKKKCIIAGVITALLVLMVVAVGITTS-----RNTSHSEKIVPVQIKTATTAVEAVCA 65

Query: 78  STDYKGKCESTLKDGVQSNPQSSDPKDLLKVAISAAVDEVKAAVKKT----TAFNFGKPE 137
            TDYK  C ++L   ++++P S+ P DL+K+  +  +  ++ ++KK     TA      +
Sbjct: 66  PTDYKETCVNSL---MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 125

Query: 138 EKAAFEDCKVLFEDALEELEKSVTQVSNNADLKKLTSRTPDLNNWLSAVMSYQETCVDGF 197
            K A E C+ L  DA ++L+K +    +   + ++     DL  WLS  ++YQ+TC+D F
Sbjct: 126 TKGALELCEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTF 185

Query: 198 PE--GKLKSDMEKALKAAKELTSNCLAMISQVASFFTSF-------EMPEGASRHLLAHD 257
            E   KL  DM+K  K ++ELTSN LAMI+ +++    F       ++ + A + L A D
Sbjct: 186 EETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED 245

Query: 258 GFPTWMTHEERRMLKGAPADKPKPNVVVAKDGSGDFKTISQALAAMPPKYEGRYVIYVKE 317
           G P+W+    RR++  A     K NVVVA DGSG +KTI++AL A+P   +  +VIY+K+
Sbjct: 246 GIPSWVGPNTRRLM--ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQ 305

Query: 318 GIYEETVVITKKMVNVTMYGDGSQKSMITGNKNFVDG-VRTFQTATFAALGDGFMAQGIG 377
           G+Y E V +TKKM +VT  GDG  K+ ITG+ N+  G V+T+ TAT A  GD F A+ IG
Sbjct: 306 GVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIG 365

Query: 378 FRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGD 437
           F NTAGPE HQAVA RV AD A+F NC+ +GYQDTLY  +HRQF+R C ++GT+DFIFGD
Sbjct: 366 FENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGD 425

Query: 438 AAAIFQNCNIMVRKPLENQQNIVTAQGRTDRHETTGIVLQNCRIQPDKTLEPVKSQFKSY 497
              + QNCNI+VRKP+++Q  ++TAQGR+D+ E+TG+VLQNC I  +    PVKS  K+Y
Sbjct: 426 GIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAY 485

Query: 498 LGRPWKEFSRTVVMESTIEDIIHPDGWLPWEGDFALSTLFYAEFNNKGAGARTNARVRWP 557
           LGRPWKEFSRT++M +TI+D+I P GWLPW GDFAL+TL+YAE+ N G G+    RV+WP
Sbjct: 486 LGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWP 545

Query: 558 GYKVIDKEEATKFTIGSFLEVD-WIESKSVPF 575
           G K +  ++A +FT   FL  + WI    VP+
Sbjct: 546 GIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of Sgr019931 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 463.4 bits (1191), Expect = 2.8e-130
Identity = 244/565 (43.19%), Postives = 355/565 (62.83%), Query Frame = 0

Query: 16  ERERKFRKRVTIAVVSTFVVLLLVAAAVVVSSKSGTANAKQPQTPATKEVSRVEKLISMI 75
           +  ++ R+ + I + S  ++ ++VA  V VS      ++K        EV+   K +  +
Sbjct: 7   DESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSK-----GKAEVNASVKAVKDV 66

Query: 76  CNSTDYKGKCESTLKDGVQSNPQSSDPKDLLKVAISAAVDEVKAAVKKT-TAFNFGKPEE 135
           C  TDY+  CE TL   +++   ++DP +L+K A +  + ++  A KK+ T     K   
Sbjct: 67  CAPTDYRKTCEDTL---IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 126

Query: 136 -KAAFEDCKVLFEDALEELEKSVTQVSNNADLKKLTSRTPDLNNWLSAVMSYQETCVDGF 195
            + A + CK L + AL+EL  S  ++    +   L     +L  WLSA +S++ETC++GF
Sbjct: 127 TRMALDQCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGF 186

Query: 196 --PEGKLKSDMEKALKAAKELTSNCLAMISQVASFFTSFEMPEGASRHLLAHDGFPTWMT 255
              +G     M+KALK A ELT N LA+IS++++F    ++P   SR LLA +GFP+W+ 
Sbjct: 187 QGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA-EGFPSWVD 246

Query: 256 HEERRMLKGAPA-DKPKPNVVVAKDGSGDFKTISQALAAMPPKYEGRYVIYVKEGIYEET 315
              R++L+ A A    KP++VVA+DGSG +KTI++AL  +P K    +V+++K G+Y+E 
Sbjct: 247 QRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEY 306

Query: 316 VVITKKMVNVTMYGDGSQKSMITGNKNFVDGVRTFQTATFAALGDGFMAQGIGFRNTAGP 375
           V + K M ++   GDG  K++I+GNKN+ DG+ T++TAT A +G+ F+A+ IGF NTAG 
Sbjct: 307 VQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGA 366

Query: 376 EKHQAVAARVQADRAIFLNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQN 435
            KHQAVA RVQ+D +IF NCRF+GYQDTLY  +HRQF+R C I+GTIDF+FGDAAA+FQN
Sbjct: 367 IKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQN 426

Query: 436 CNIMVRKPLENQQNIVTAQGRTDRHETTGIVLQNCRIQPDKTLEPVKSQFKSYLGRPWKE 495
           C ++VRKPL NQ   +TA GR D  E+TG V Q C I  +     VK   K+YLGRPWKE
Sbjct: 427 CTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKE 486

Query: 496 FSRTVVMESTIEDIIHPDGWLPWEGDFALSTLFYAEFNNKGAGARTNARVRWPGYKVIDK 555
           +SRT++M + I D + P GW PW GDF L TLFY+E  N G G+    RV W G K + +
Sbjct: 487 YSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSE 546

Query: 556 EEATKFTIGSFLE-VDWIESKSVPF 575
           E+  KFT   +++  DWI  K VP+
Sbjct: 547 EDILKFTPAQYIQGDDWIPGKGVPY 561