Sgr019418 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr019418
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionphospholipase A1-Igamma1, chloroplastic-like
Locationtig00153347: 396480 .. 398095 (+)
RNA-Seq ExpressionSgr019418
SyntenySgr019418
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAGAAAGTCGTTACGCCTAGGGCGTCTACGCCAACGAAACGAGGTGTACCCTTGAGAAAAGTGACGATGTTGAGAAATGGAACGAAGAAGACGAAGTGGTACTGGAAATTGAAGTTCGGGATCCGACTGCAAGCCATTAAAAAGGCTCTTGCATCTTCCTTACACCAGCAGCGTCTACATCTCACATGTTCTACCGCCATGAATGGCCAGCTCACTCCTGCAAACGGCGCCGTCTCGCCACTGGTTATTCAGCCGTCGAAATCGTCGTCGAAGAAGAACGATCTTCGTCTCGCGAAACCACTAGCCTCCCTCCTCCGTATGCCACTCCGAGCTATAGATTTCATCGACTATGGCAACCACATGACCCCGACACTGTCTCCCCGAGAAAATATCACCGCCGTGTGGCGCGAACTCCACGGCCTCCGCGACTGGGACGGCCTCCTCGACCCCCTCCACCCCTTTCTCCGGCGAGAGCTCGTCAAGTACGGCGAGTTCGCCCAAGCCACCTACGACGCCTTCGACTTCGACTCACTATCCGAATTCTGCGGGAGTTGCAGATACAACCGCCACAAGCTTTTACAAGAACTGGGTCTGGCGCAGAACGGATACAAAGTCACCAAATACATCTACGCTTTGTCGCCGGTGGACGGGCCGGAGTGGTTCGAGAGCTCGAAGATCGGCGAAGTTTGGAGCAGAGATTCAAACTGGATGGGGTTCGTCGCAGTCAGCAACGACGAAGAGAGCGAGAGAATTGGAAGACGAGACATCCTCGTGGCGTGGAGAGGCACCGTAACTCCAACCGAATGGTACATCGATCTCAAAACCAAGCTGAAGAAAATCGACCGGGAAAACAAGAAAGTCAAAGTCCAACGTGGGTTTCTAACTATTTACAGATCCAACGACGAGCAATCCAGATTCAACAAGACCAGCGCGTCGGAGCAAGTGATGGAAGAGTTATACAGACTGGTCGAGTTCTTCAAACAAAAAGGCGACAGAGAAATCAGCATAACGATCACCGGACACAGCCTCGGCGGCGCGCTGTCTCTCTTGAACGCGTACGAAGCCGGAGTTTCCTTCCCCGACGTCCATATCAGCGTGGTTTCATTCGGAGCTCCGAGGGCCGGAAACTTAGCGTTCAAAGAGAAGCTCAACGAGCTGGGTGTGAAAACCCTAAGAGTGGTGGTCCGACAAGACATCGTCCCGAAGCTTCCGGGGCTGGTGGTGAACTCGATCGTGAACAAGCTCAGCGCCATAACCTCGAAGCTGAACTGGGTTTACCGGCACGTGGGGACTCAGCTGAAGATGGACATGTTCATGTCGCCGTATCTGAAGCACGAGTCGGACATGTCGGGGAGCCATAACCTGGAGATTTATCTGCATCTGGTGGACGGATTTGTGAGCGGCGAGGGAAGTTCCGGTGGAACTCGAGGAGGGACGTGGCGCTGGTGAATAAGGGGAGCGATATGCTGGTGGAGGAGCTGAGGATACCGGAGTTCTGGAACCAGTTGCCACACAAGGGGCTGGTGAAGAACAGATATGGAAGATGGGTTAAGCCTGGGAGGAACCCTGAGGATGTTCCTTCTCCATTTTCGCAACCTTCTCAGATTTGA

mRNA sequence

ATGGAGGAGAAAGTCGTTACGCCTAGGGCGTCTACGCCAACGAAACGAGGTGTACCCTTGAGAAAAGTGACGATGTTGAGAAATGGAACGAAGAAGACGAAGTGGTACTGGAAATTGAAGTTCGGGATCCGACTGCAAGCCATTAAAAAGGCTCTTGCATCTTCCTTACACCAGCAGCGTCTACATCTCACATGTTCTACCGCCATGAATGGCCAGCTCACTCCTGCAAACGGCGCCGTCTCGCCACTGGTTATTCAGCCGTCGAAATCGTCGTCGAAGAAGAACGATCTTCGTCTCGCGAAACCACTAGCCTCCCTCCTCCGTATGCCACTCCGAGCTATAGATTTCATCGACTATGGCAACCACATGACCCCGACACTGTCTCCCCGAGAAAATATCACCGCCGTGTGGCGCGAACTCCACGGCCTCCGCGACTGGGACGGCCTCCTCGACCCCCTCCACCCCTTTCTCCGGCGAGAGCTCGTCAAGTACGGCGAGTTCGCCCAAGCCACCTACGACGCCTTCGACTTCGACTCACTATCCGAATTCTGCGGGAGTTGCAGATACAACCGCCACAAGCTTTTACAAGAACTGGGTCTGGCGCAGAACGGATACAAAGTCACCAAATACATCTACGCTTTGTCGCCGGTGGACGGGCCGGAGTGGTTCGAGAGCTCGAAGATCGGCGAAGTTTGGAGCAGAGATTCAAACTGGATGGGGTTCGTCGCAGTCAGCAACGACGAAGAGAGCGAGAGAATTGGAAGACGAGACATCCTCGTGGCGTGGAGAGGCACCGTAACTCCAACCGAATGGTACATCGATCTCAAAACCAAGCTGAAGAAAATCGACCGGGAAAACAAGAAAGTCAAAGTCCAACGTGGGTTTCTAACTATTTACAGATCCAACGACGAGCAATCCAGATTCAACAAGACCAGCGCGTCGGAGCAAGTGATGGAAGAGTTATACAGACTGGTCGAGTTCTTCAAACAAAAAGGCGACAGAGAAATCAGCATAACGATCACCGGACACAGCCTCGGCGGCGCGCTGTCTCTCTTGAACGCGTACGAAGCCGGAGTTTCCTTCCCCGACGTCCATATCAGCGTGGTTTCATTCGGAGCTCCGAGGGCCGGAAACTTAGCGTTCAAAGAGAAGCTCAACGAGCTGGGTGTGAAAACCCTAAGAGTGGTGGTCCGACAAGACATCGTCCCGAAGCTTCCGGGGCTGGTGGTGAACTCGATCGTGAACAAGCTCAGCGCCATAACCTCGAAGCTGAACTGGGTTTACCGGCACGTGGGGACTCAGCTGAAGATGGACATGTTCATGTCGCCGTATCTGAAGCACGAGTCGGACATGTCGGGGAGCCATAACCTGGAGATTTATCTGCATCTGGTGGACGGATTTGGGAGCGATATGCTGGTGGAGGAGCTGAGGATACCGGAGTTCTGGAACCAGTTGCCACACAAGGGGCTGGTGAAGAACAGATATGGAAGATGGGTTAAGCCTGGGAGGAACCCTGAGGATGTTCCTTCTCCATTTTCGCAACCTTCTCAGATTTGA

Coding sequence (CDS)

ATGGAGGAGAAAGTCGTTACGCCTAGGGCGTCTACGCCAACGAAACGAGGTGTACCCTTGAGAAAAGTGACGATGTTGAGAAATGGAACGAAGAAGACGAAGTGGTACTGGAAATTGAAGTTCGGGATCCGACTGCAAGCCATTAAAAAGGCTCTTGCATCTTCCTTACACCAGCAGCGTCTACATCTCACATGTTCTACCGCCATGAATGGCCAGCTCACTCCTGCAAACGGCGCCGTCTCGCCACTGGTTATTCAGCCGTCGAAATCGTCGTCGAAGAAGAACGATCTTCGTCTCGCGAAACCACTAGCCTCCCTCCTCCGTATGCCACTCCGAGCTATAGATTTCATCGACTATGGCAACCACATGACCCCGACACTGTCTCCCCGAGAAAATATCACCGCCGTGTGGCGCGAACTCCACGGCCTCCGCGACTGGGACGGCCTCCTCGACCCCCTCCACCCCTTTCTCCGGCGAGAGCTCGTCAAGTACGGCGAGTTCGCCCAAGCCACCTACGACGCCTTCGACTTCGACTCACTATCCGAATTCTGCGGGAGTTGCAGATACAACCGCCACAAGCTTTTACAAGAACTGGGTCTGGCGCAGAACGGATACAAAGTCACCAAATACATCTACGCTTTGTCGCCGGTGGACGGGCCGGAGTGGTTCGAGAGCTCGAAGATCGGCGAAGTTTGGAGCAGAGATTCAAACTGGATGGGGTTCGTCGCAGTCAGCAACGACGAAGAGAGCGAGAGAATTGGAAGACGAGACATCCTCGTGGCGTGGAGAGGCACCGTAACTCCAACCGAATGGTACATCGATCTCAAAACCAAGCTGAAGAAAATCGACCGGGAAAACAAGAAAGTCAAAGTCCAACGTGGGTTTCTAACTATTTACAGATCCAACGACGAGCAATCCAGATTCAACAAGACCAGCGCGTCGGAGCAAGTGATGGAAGAGTTATACAGACTGGTCGAGTTCTTCAAACAAAAAGGCGACAGAGAAATCAGCATAACGATCACCGGACACAGCCTCGGCGGCGCGCTGTCTCTCTTGAACGCGTACGAAGCCGGAGTTTCCTTCCCCGACGTCCATATCAGCGTGGTTTCATTCGGAGCTCCGAGGGCCGGAAACTTAGCGTTCAAAGAGAAGCTCAACGAGCTGGGTGTGAAAACCCTAAGAGTGGTGGTCCGACAAGACATCGTCCCGAAGCTTCCGGGGCTGGTGGTGAACTCGATCGTGAACAAGCTCAGCGCCATAACCTCGAAGCTGAACTGGGTTTACCGGCACGTGGGGACTCAGCTGAAGATGGACATGTTCATGTCGCCGTATCTGAAGCACGAGTCGGACATGTCGGGGAGCCATAACCTGGAGATTTATCTGCATCTGGTGGACGGATTTGGGAGCGATATGCTGGTGGAGGAGCTGAGGATACCGGAGTTCTGGAACCAGTTGCCACACAAGGGGCTGGTGAAGAACAGATATGGAAGATGGGTTAAGCCTGGGAGGAACCCTGAGGATGTTCCTTCTCCATTTTCGCAACCTTCTCAGATTTGA

Protein sequence

MEEKVVTPRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQRLHLTCSTAMNGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDFIDYGNHMTPTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFLTIYRSNDEQSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVSFPDVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAITSKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGFGSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQPSQI
Homology
BLAST of Sgr019418 vs. NCBI nr
Match: XP_022927629.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 901.4 bits (2328), Expect = 3.7e-258
Identity = 442/538 (82.16%), Postives = 479/538 (89.03%), Query Frame = 0

Query: 1   MEEKVVTPRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQR 60
           ME ++VTP       RGVPL++VTMLRNGTKKTKWYWKLK+GIRL+AIK AL+SSLH QR
Sbjct: 1   MEGRIVTP-------RGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQR 60

Query: 61  LHLTCSTAMNGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDFIDYG 120
             LTC+TA  GQLTP NGAVSPLVIQPSKS+SKKNDLRLAKPLASLLRMP RA DF+DYG
Sbjct: 61  RRLTCATA-TGQLTPVNGAVSPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYG 120

Query: 121 NHMTPTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSL 180
           NHMTPT SPR+ I  VWR+LHG  +W+GLLDPLHPFLRRELVKYGEFAQATYDAFDFD L
Sbjct: 121 NHMTPTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPL 180

Query: 181 SEFCGSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMG 240
           SEF GSCRYNRHKLL ELGLAQNGYKVTKYIYALSPVDGP+WFESSKIGEVWSRDSNWMG
Sbjct: 181 SEFRGSCRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMG 240

Query: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFLTIYR 300
           FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR ++K+KVQRGFLTIY+
Sbjct: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYK 300

Query: 301 SNDEQSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVS 360
           S DE+S+FNKTSASEQVMEEL+RL++FF QKGDREIS+TI GHSLGGALSLL AYEAG S
Sbjct: 301 SKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGAS 360

Query: 361 FPDVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAI 420
           FPD+H+SV+SFGAPR GNLAF+EKLNE+GVKTLRVV+RQDIVPKLPGL VNSIVNKLSA+
Sbjct: 361 FPDIHVSVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 420

Query: 421 TSKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF------------- 480
           T KLNW+YRHVGTQLKMDM+MSPYLK ESDMSGSHNLEIYLHLVDGF             
Sbjct: 421 TGKLNWLYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRR 480

Query: 481 -------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQPSQI 519
                  GSDMLVEELRIPEFW QLPHKGLVKNR+GRWVKPGRN ED+PSPFS+PS I
Sbjct: 481 DVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Sgr019418 vs. NCBI nr
Match: KAG6583869.1 (Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 901.0 bits (2327), Expect = 4.9e-258
Identity = 442/538 (82.16%), Postives = 478/538 (88.85%), Query Frame = 0

Query: 1   MEEKVVTPRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQR 60
           ME ++VTP       RGVPL++VTMLRNGTKKTKWYWKLK+GIRL+AIK ALASSLH QR
Sbjct: 1   MEGRIVTP-------RGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALASSLHNQR 60

Query: 61  LHLTCSTAMNGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDFIDYG 120
             LTC+TA  GQLTP NGA SPLVIQPSKS+SKKNDLRLAKPLASLLRMP RA DF+DYG
Sbjct: 61  RRLTCATA-TGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYG 120

Query: 121 NHMTPTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSL 180
           NHMTPT SPR+ I  VWR+LHG  +W+GLLDPLHPFLRRELVKYGEFAQATYDAFDFD L
Sbjct: 121 NHMTPTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPL 180

Query: 181 SEFCGSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMG 240
           SEF GSCRYNRHKLL ELGLAQNGYKVTKYIYALSPVDGP+WFESSKIGEVWSRDSNWMG
Sbjct: 181 SEFRGSCRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMG 240

Query: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFLTIYR 300
           FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR ++K+KVQRGFLTIY+
Sbjct: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYK 300

Query: 301 SNDEQSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVS 360
           S DE+S+FNKTSASEQVMEEL+RL++FF QKGDREIS+TI GHSLGGALSLL AYEAG S
Sbjct: 301 SKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGAS 360

Query: 361 FPDVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAI 420
           FPD+H+SV+SFGAPR GNLAF+EKLNE+GVKTLRVV+RQDIVPKLPGL VNSIVNKLSA+
Sbjct: 361 FPDIHVSVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 420

Query: 421 TSKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF------------- 480
           T KLNW+YRHVGTQLKMDM+MSPYLK ESDMSGSHNLEIYLHLVDGF             
Sbjct: 421 TGKLNWLYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRR 480

Query: 481 -------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQPSQI 519
                  GSDMLVEELRIPEFW QLPHKGLVKNR+GRWVKPGRN ED+PSPFS+PS I
Sbjct: 481 DVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Sgr019418 vs. NCBI nr
Match: KAG7019492.1 (Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 899.8 bits (2324), Expect = 1.1e-257
Identity = 441/538 (81.97%), Postives = 478/538 (88.85%), Query Frame = 0

Query: 1   MEEKVVTPRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQR 60
           ME ++VTP       RGVPL++VTMLRNGTKKTKWYWKLK+GIRL+AIK AL+SSLH QR
Sbjct: 1   MEGRIVTP-------RGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQR 60

Query: 61  LHLTCSTAMNGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDFIDYG 120
             LTC+TA  GQLTP NGA SPLVIQPSKS+SKKNDLRLAKPLASLLRMP RA DF+DYG
Sbjct: 61  RRLTCATA-TGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYG 120

Query: 121 NHMTPTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSL 180
           NHMTPT SPR+ I  VWR+LHG  +W+GLLDPLHPFLRRELVKYGEFAQATYDAFDFD L
Sbjct: 121 NHMTPTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPL 180

Query: 181 SEFCGSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMG 240
           SEF GSCRYNRHKLL ELGLAQNGYKVTKYIYALSPVDGP+WFESSKIGEVWSRDSNWMG
Sbjct: 181 SEFRGSCRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMG 240

Query: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFLTIYR 300
           FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR ++K+KVQRGFLTIY+
Sbjct: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYK 300

Query: 301 SNDEQSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVS 360
           S DE+S+FNKTSASEQVMEEL+RL++FF QKGDREIS+TI GHSLGGALSLL AYEAG S
Sbjct: 301 SKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGAS 360

Query: 361 FPDVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAI 420
           FPD+H+SV+SFGAPR GNLAF+EKLNE+GVKTLRVV+RQDIVPKLPGL VNSIVNKLSA+
Sbjct: 361 FPDIHVSVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 420

Query: 421 TSKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF------------- 480
           T KLNW+YRHVGTQLKMDM+MSPYLK ESDMSGSHNLEIYLHLVDGF             
Sbjct: 421 TGKLNWLYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRR 480

Query: 481 -------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQPSQI 519
                  GSDMLVEELRIPEFW QLPHKGLVKNR+GRWVKPGRN ED+PSPFS+PS I
Sbjct: 481 DVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Sgr019418 vs. NCBI nr
Match: XP_023519232.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 898.3 bits (2320), Expect = 3.2e-257
Identity = 440/538 (81.78%), Postives = 478/538 (88.85%), Query Frame = 0

Query: 1   MEEKVVTPRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQR 60
           ME ++VTP       RGVPL++VTMLRNGTKKTKWYWKLK+GIRL+AIK AL+SSLH QR
Sbjct: 1   MEGRIVTP-------RGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQR 60

Query: 61  LHLTCSTAMNGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDFIDYG 120
             LTC+TA  GQLTP NGA SPLVIQPSKS+SKKNDLRLAKPLASLLRMP RA DF+DYG
Sbjct: 61  RRLTCATA-TGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYG 120

Query: 121 NHMTPTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSL 180
           NHMTPT SPR+ I  VWR+LHG  +W+GLLDPLHPFLRRELVKYGEFAQATYDAFDFD L
Sbjct: 121 NHMTPTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPL 180

Query: 181 SEFCGSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMG 240
           SEF GSCRYNRHKLL ELGLAQNGYKVT+YIYALSPVDGP+WFESSKIGEVWSRDSNWMG
Sbjct: 181 SEFRGSCRYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMG 240

Query: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFLTIYR 300
           FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR ++K+KVQRGFLTIY+
Sbjct: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYK 300

Query: 301 SNDEQSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVS 360
           S DE+S+FNKTSASEQVMEEL+RL++FF QKGDREIS+TI GHSLGGALSLL AYEAG S
Sbjct: 301 SKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGAS 360

Query: 361 FPDVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAI 420
           FPD+H+SV+SFGAPR GNLAF+EKLNE+GVKTLRVV+RQDIVPKLPGL VNSIVNKLSA+
Sbjct: 361 FPDIHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 420

Query: 421 TSKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF------------- 480
           T KLNW+YRHVGTQLKMDM+MSPYLK ESDMSGSHNLEIYLHLVDGF             
Sbjct: 421 TGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRR 480

Query: 481 -------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQPSQI 519
                  GSDMLVEELRIPEFW QLPHKGLVKNR+GRWVKPGRN ED+PSPFS+PS I
Sbjct: 481 DVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Sgr019418 vs. NCBI nr
Match: XP_023001410.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 897.5 bits (2318), Expect = 5.4e-257
Identity = 440/538 (81.78%), Postives = 477/538 (88.66%), Query Frame = 0

Query: 1   MEEKVVTPRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQR 60
           ME ++VTP       RGVPL++VTMLRNGTKKTKWYWKLK+GIRL+AIK AL+SSLH QR
Sbjct: 1   MEGRIVTP-------RGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQR 60

Query: 61  LHLTCSTAMNGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDFIDYG 120
             LTCSTA  GQLTP NGA SPLVIQPSKS++KKNDLRLAKPLASLLRMP RA DF+DYG
Sbjct: 61  RRLTCSTA-TGQLTPVNGAASPLVIQPSKSTAKKNDLRLAKPLASLLRMPQRAADFVDYG 120

Query: 121 NHMTPTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSL 180
           NHMTPT SPR+ I  VWR+LHG  +W+GLLDPLHPFLRRELVKYGEFAQATYDAFDFD L
Sbjct: 121 NHMTPTFSPRDKIADVWRDLHGASNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPL 180

Query: 181 SEFCGSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMG 240
           SE+ GSCRYNRHKLL ELGLAQNGYKVTKYIYALSPVDGP+WFESSKIGEVWSRDSNWMG
Sbjct: 181 SEYRGSCRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMG 240

Query: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFLTIYR 300
           FVAVS+DEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR +KK+KVQRGFLTIY+
Sbjct: 241 FVAVSDDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKIKVQRGFLTIYK 300

Query: 301 SNDEQSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVS 360
           S DE+S+FNKTSASEQVMEEL+RL+ FF QKGDREIS+TI GHSLGGALSLL AYEAGVS
Sbjct: 301 SKDEESKFNKTSASEQVMEELHRLIGFFTQKGDREISLTIVGHSLGGALSLLTAYEAGVS 360

Query: 361 FPDVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAI 420
           FPD+H+SV+SFGAPR GNLAF+EKLNE+GVKTLRVV+RQDIVPKLPGL VNSIVNKLSA+
Sbjct: 361 FPDIHVSVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 420

Query: 421 TSKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF------------- 480
           T KLNW+YRHVGTQLKMDM+MSPYLK ESDMSGSHNLEIYLHLVDGF             
Sbjct: 421 TGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRR 480

Query: 481 -------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQPSQI 519
                  GSDMLVEELRIPEFW QLPHKGLVKNR+GRWVKPGRN ED+PSPF +PS I
Sbjct: 481 DVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFPRPSNI 530

BLAST of Sgr019418 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 355.1 bits (910), Expect = 1.3e-96
Identity = 187/411 (45.50%), Postives = 258/411 (62.77%), Query Frame = 0

Query: 137 WRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRYNRHKLLQ 196
           WR++ G  DW GL+DP+ P LR EL++YGE AQA YDAFDFD  S++CG+ R+ R +   
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 197 ELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEES-ERIGR 256
            LG+  +GY+V +Y+YA S ++ P +F  S+  +VWS+++NWMG+VAVS+DE S  R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 257 RDILVAWRGTVTPTEWYIDLKTKLKKIDRENK------KVKVQRGFLTIYRSNDEQSRFN 316
           RDI +AWRGTVT  EW  DLK  LK +  ENK       VKV+ GFL +Y   D   +F 
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPV-TENKIRCPDPAVKVESGFLDLYTDKDTTCKFA 284

Query: 317 KTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVSFPD------ 376
           + SA EQ++ E+ RLVE      D ++SIT+TGHSLGGAL++L+AY+      +      
Sbjct: 285 RFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGK 344

Query: 377 -VHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVN-SIVNKLSAIT 436
            + ++V+++G PR GN+ F+E++ ELGVK +RVV   D+VPK PGL +N S  + L  I 
Sbjct: 345 VIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIA 404

Query: 437 SKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF-------------- 496
             L W Y HVG +L +D   SP+LK   D+S +HNLE  LHL+DG+              
Sbjct: 405 EGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLSSGRD 464

Query: 497 ------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGR-NPEDVPSP 512
                  SD L E L+IP FW Q  +KG+V+N  GRW++  R   ED  SP
Sbjct: 465 HALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of Sgr019418 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 353.6 bits (906), Expect = 3.8e-96
Identity = 180/402 (44.78%), Postives = 247/402 (61.44%), Query Frame = 0

Query: 137 WRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRYNRHKLLQ 196
           WR++ G  DW GL+DP+ P LR EL++YGE AQA YDAFDFD  S +CGSCR+ R  L  
Sbjct: 89  WRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFD 148

Query: 197 ELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEESE--RIG 256
            LG+  +GY+V +Y+YA S ++ P +F  S+  +VWS+++NWMG+VAVS+D E+   R+G
Sbjct: 149 SLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLG 208

Query: 257 RRDILVAWRGTVTPTEWYIDLKTKLKKIDRE-----NKKVKVQRGFLTIYRSNDEQSRFN 316
           RRDI +AWRGTVT  EW  DLK  LK +        +  VK + GFL +Y   D    F+
Sbjct: 209 RRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFS 268

Query: 317 KTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAY---EAGVSFPD--- 376
           K SA EQV+ E+ RLVE +  +   E+SIT+TGHSLGGAL++L+AY   E GV+      
Sbjct: 269 KFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGK 328

Query: 377 -VHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNS-IVNKLSAIT 436
            + ++  ++G PR GN+ FKE++ +LGVK LRVV   D+V K PGL +N      L  + 
Sbjct: 329 VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLA 388

Query: 437 SKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF-------------- 496
             L W Y HVG  L +D   SP+LK   D+S +HNLE  LHL+DG+              
Sbjct: 389 GGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSSGRD 448

Query: 497 ------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGR 504
                  SD L +   +P +W Q  +KG+V+N  GRW++P R
Sbjct: 449 PALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDR 490

BLAST of Sgr019418 vs. ExPASy Swiss-Prot
Match: Q9C8J6 (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2 PE=1 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 5.9e-89
Identity = 175/427 (40.98%), Postives = 251/427 (58.78%), Query Frame = 0

Query: 132 NITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRYNR 191
           ++  +WRE+ G  +W+G LDP++  LRRE+++YGEFAQA YD+FDFD  S++CGSC+Y+ 
Sbjct: 86  SLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHP 145

Query: 192 HKLLQELGL-AQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEES 251
                 L L    GY +T+Y+YA S ++ P +F+ SK+  +WS+ +NWMGFVAV+ DEE 
Sbjct: 146 SDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEE 205

Query: 252 -ERIGRRDILVAWRGTVTPTEWYIDLKTKLKKID-RENKKVKVQRGFLTIYRSNDEQSRF 311
             R+GRRDI++AWRGTVT  EW  DLK  L   +  ++  +K++ GF  +Y   ++  +F
Sbjct: 206 VSRLGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKF 265

Query: 312 NKTSASEQVMEELYRLVEFF-KQKGDREISITITGHSLGGALSLLNAYEAGV----SFPD 371
           +  SA EQV+ E+ RL+E++  ++   + SIT+TGHSLG +L+L++AY+         P+
Sbjct: 266 SSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPE 325

Query: 372 ----VHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSA 431
               + I+V SF  PR GNL FKE+ +ELGVK LRVV   D VP +PG+  N        
Sbjct: 326 NNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKY 385

Query: 432 ITSKLN--WVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF---------- 491
           +  K +  W Y HVG +L +D   SP+LK   D+  +HNLE  LHLVDG+          
Sbjct: 386 VEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKR 445

Query: 492 --------------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGR------NPED 515
                           D L  E  +P  W Q  +KG+VKN  G+WV P R       PED
Sbjct: 446 FCLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGPED 505

BLAST of Sgr019418 vs. ExPASy Swiss-Prot
Match: A2WY00 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=EG1 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.5e-64
Identity = 141/345 (40.87%), Postives = 207/345 (60.00%), Query Frame = 0

Query: 131 ENITAVWRELHGLRDWDGLLDPL-HPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRY 190
           E +  +WRE+ G  DW+G+L+P  HP LR E+ +YGE   A Y AFD D  S    +C+Y
Sbjct: 54  ETVAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFDLDPASRRYLNCKY 113

Query: 191 NRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEE 250
            R ++L+E+G+   GY+VT+YIYA + V  P    S+      S    W+G+VAVS DE 
Sbjct: 114 GRERMLEEVGMGGAGYEVTRYIYAAADVSVPTMEPST------SGRGRWIGYVAVSTDEM 173

Query: 251 SERIGRRDILVAWRGTVTPTEWYIDLKTKLK--KIDRENKK--VKVQRGFLTIYRSNDEQ 310
           S R+GRRD+LV++RGTVTP EW  +L + L+  ++D  + +  VKV+ GFL++Y S D+ 
Sbjct: 174 SRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLSLYTSADKT 233

Query: 311 SRFNKT-SASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAY---EAGVSF 370
            RF    S  EQ++ E+ RLV  +   G+ ++S+T+ GHS+G AL+LL+AY   E G++ 
Sbjct: 234 CRFGGAGSCREQLLREVSRLVAAYSGGGE-DVSVTLAGHSMGSALALLSAYDLAELGLN- 293

Query: 371 PDVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAIT 430
               ++V SFG PR GN AFK + +ELGVK LRV    D + KLPG+ +N        + 
Sbjct: 294 RAAPVTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFLNEAT--AGVLR 353

Query: 431 SKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDG 467
              +  Y HVG +L +D F         D++  H+L  Y+ L+ G
Sbjct: 354 PWRHSCYTHVGVELPLDFF------KVGDLASVHDLATYISLLRG 382

BLAST of Sgr019418 vs. ExPASy Swiss-Prot
Match: Q8S1D9 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=EG1 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.5e-64
Identity = 141/345 (40.87%), Postives = 207/345 (60.00%), Query Frame = 0

Query: 131 ENITAVWRELHGLRDWDGLLDPL-HPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRY 190
           E +  +WRE+ G  DW+G+L+P  HP LR E+ +YGE   A Y AFD D  S    +C+Y
Sbjct: 54  ETVAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFDLDPASRRYLNCKY 113

Query: 191 NRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEE 250
            R ++L+E+G+   GY+VT+YIYA + V  P    S+      S    W+G+VAVS DE 
Sbjct: 114 GRERMLEEVGMGGAGYEVTRYIYAAADVSVPTMEPST------SGRGRWIGYVAVSTDEM 173

Query: 251 SERIGRRDILVAWRGTVTPTEWYIDLKTKLK--KIDRENKK--VKVQRGFLTIYRSNDEQ 310
           S R+GRRD+LV++RGTVTP EW  +L + L+  ++D  + +  VKV+ GFL++Y S D+ 
Sbjct: 174 SRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLSLYTSADKT 233

Query: 311 SRFNKT-SASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAY---EAGVSF 370
            RF    S  EQ++ E+ RLV  +   G+ ++S+T+ GHS+G AL+LL+AY   E G++ 
Sbjct: 234 CRFGGAGSCREQLLREVSRLVAAYSGGGE-DVSVTLAGHSMGSALALLSAYDLAELGLN- 293

Query: 371 PDVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAIT 430
               ++V SFG PR GN AFK + +ELGVK LRV    D + KLPG+ +N        + 
Sbjct: 294 RAAPVTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFLNEAT--AGVLR 353

Query: 431 SKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDG 467
              +  Y HVG +L +D F         D++  H+L  Y+ L+ G
Sbjct: 354 PWRHSCYTHVGVELPLDFF------KVGDLASVHDLATYISLLRG 382

BLAST of Sgr019418 vs. ExPASy TrEMBL
Match: A0A6J1EIJ3 (phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434398 PE=4 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 1.8e-258
Identity = 442/538 (82.16%), Postives = 479/538 (89.03%), Query Frame = 0

Query: 1   MEEKVVTPRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQR 60
           ME ++VTP       RGVPL++VTMLRNGTKKTKWYWKLK+GIRL+AIK AL+SSLH QR
Sbjct: 1   MEGRIVTP-------RGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQR 60

Query: 61  LHLTCSTAMNGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDFIDYG 120
             LTC+TA  GQLTP NGAVSPLVIQPSKS+SKKNDLRLAKPLASLLRMP RA DF+DYG
Sbjct: 61  RRLTCATA-TGQLTPVNGAVSPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYG 120

Query: 121 NHMTPTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSL 180
           NHMTPT SPR+ I  VWR+LHG  +W+GLLDPLHPFLRRELVKYGEFAQATYDAFDFD L
Sbjct: 121 NHMTPTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPL 180

Query: 181 SEFCGSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMG 240
           SEF GSCRYNRHKLL ELGLAQNGYKVTKYIYALSPVDGP+WFESSKIGEVWSRDSNWMG
Sbjct: 181 SEFRGSCRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMG 240

Query: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFLTIYR 300
           FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR ++K+KVQRGFLTIY+
Sbjct: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYK 300

Query: 301 SNDEQSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVS 360
           S DE+S+FNKTSASEQVMEEL+RL++FF QKGDREIS+TI GHSLGGALSLL AYEAG S
Sbjct: 301 SKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGAS 360

Query: 361 FPDVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAI 420
           FPD+H+SV+SFGAPR GNLAF+EKLNE+GVKTLRVV+RQDIVPKLPGL VNSIVNKLSA+
Sbjct: 361 FPDIHVSVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 420

Query: 421 TSKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF------------- 480
           T KLNW+YRHVGTQLKMDM+MSPYLK ESDMSGSHNLEIYLHLVDGF             
Sbjct: 421 TGKLNWLYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRR 480

Query: 481 -------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQPSQI 519
                  GSDMLVEELRIPEFW QLPHKGLVKNR+GRWVKPGRN ED+PSPFS+PS I
Sbjct: 481 DVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Sgr019418 vs. ExPASy TrEMBL
Match: A0A6J1KL46 (phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495553 PE=4 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.6e-257
Identity = 440/538 (81.78%), Postives = 477/538 (88.66%), Query Frame = 0

Query: 1   MEEKVVTPRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQR 60
           ME ++VTP       RGVPL++VTMLRNGTKKTKWYWKLK+GIRL+AIK AL+SSLH QR
Sbjct: 1   MEGRIVTP-------RGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQR 60

Query: 61  LHLTCSTAMNGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDFIDYG 120
             LTCSTA  GQLTP NGA SPLVIQPSKS++KKNDLRLAKPLASLLRMP RA DF+DYG
Sbjct: 61  RRLTCSTA-TGQLTPVNGAASPLVIQPSKSTAKKNDLRLAKPLASLLRMPQRAADFVDYG 120

Query: 121 NHMTPTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSL 180
           NHMTPT SPR+ I  VWR+LHG  +W+GLLDPLHPFLRRELVKYGEFAQATYDAFDFD L
Sbjct: 121 NHMTPTFSPRDKIADVWRDLHGASNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPL 180

Query: 181 SEFCGSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMG 240
           SE+ GSCRYNRHKLL ELGLAQNGYKVTKYIYALSPVDGP+WFESSKIGEVWSRDSNWMG
Sbjct: 181 SEYRGSCRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMG 240

Query: 241 FVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFLTIYR 300
           FVAVS+DEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR +KK+KVQRGFLTIY+
Sbjct: 241 FVAVSDDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKIKVQRGFLTIYK 300

Query: 301 SNDEQSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVS 360
           S DE+S+FNKTSASEQVMEEL+RL+ FF QKGDREIS+TI GHSLGGALSLL AYEAGVS
Sbjct: 301 SKDEESKFNKTSASEQVMEELHRLIGFFTQKGDREISLTIVGHSLGGALSLLTAYEAGVS 360

Query: 361 FPDVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAI 420
           FPD+H+SV+SFGAPR GNLAF+EKLNE+GVKTLRVV+RQDIVPKLPGL VNSIVNKLSA+
Sbjct: 361 FPDIHVSVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 420

Query: 421 TSKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF------------- 480
           T KLNW+YRHVGTQLKMDM+MSPYLK ESDMSGSHNLEIYLHLVDGF             
Sbjct: 421 TGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRR 480

Query: 481 -------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQPSQI 519
                  GSDMLVEELRIPEFW QLPHKGLVKNR+GRWVKPGRN ED+PSPF +PS I
Sbjct: 481 DVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFPRPSNI 530

BLAST of Sgr019418 vs. ExPASy TrEMBL
Match: A0A5A7UFJ9 (Phospholipase A1-Igamma1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001160 PE=4 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.6e-254
Identity = 437/543 (80.48%), Postives = 477/543 (87.85%), Query Frame = 0

Query: 1   MEEKVVTPRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQR 60
           ME K+ TP       RG+P+++ TMLRNGTKKTKWYWKLK GIR +AIK AL+SSLH QR
Sbjct: 1   MEAKLATP-------RGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQR 60

Query: 61  LHLTCSTAM----NGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDF 120
             LTCSTA+      QLTP NG VSPLVI  SK  SKKNDLRLAKPLASLLRMPLRA DF
Sbjct: 61  RRLTCSTAITTTTTPQLTPTNGLVSPLVIHQSK-PSKKNDLRLAKPLASLLRMPLRAADF 120

Query: 121 IDYGNHMTPTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFD 180
           ID+GNHMTPTLSPR+NI+AVWR+LHG  DW+GLLDPLHPFLRRE+VKYGEFAQATYDAFD
Sbjct: 121 IDFGNHMTPTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFD 180

Query: 181 FDSLSEFCGSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDS 240
           FD LSEFCGSCRYNRHKLL ELGLAQNGYKVTKYIYALSPVDGP+WFESSKIGEVWSRDS
Sbjct: 181 FDPLSEFCGSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDS 240

Query: 241 NWMGFVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFL 300
           NWMG+VAVS+DEES+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR NKKVKVQRGFL
Sbjct: 241 NWMGYVAVSSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFL 300

Query: 301 TIYRSNDEQSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYE 360
           TIY+S DE S+FNKTSASEQVMEEL+RL++FFK+KGDREIS+TITGHSLGGALSLL AYE
Sbjct: 301 TIYKSKDEDSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTAYE 360

Query: 361 AGVSFP-DVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVN 420
           AGV+FP DVH+SVVSFGAPR GNLAF+EKLNE+GVKTLRVV+RQDIVPKLPGL VNSIVN
Sbjct: 361 AGVTFPADVHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVN 420

Query: 421 KLSAITSKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF-------- 480
           KL A+T KLNWVYRHVG +L+M+M+MSPYLK +SDMSGSHNLEIYLHLVDGF        
Sbjct: 421 KLGAVTGKLNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGKFR 480

Query: 481 ------------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQP 519
                       GSDMLVEELRIPEFW QLPHKGL+KNR+GRWVKPGRNPED+PSPFSQP
Sbjct: 481 WNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQP 535

BLAST of Sgr019418 vs. ExPASy TrEMBL
Match: A0A1S3BBX3 (phospholipase A1-Igamma1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487991 PE=4 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.6e-254
Identity = 437/543 (80.48%), Postives = 477/543 (87.85%), Query Frame = 0

Query: 1   MEEKVVTPRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQR 60
           ME K+ TP       RG+P+++ TMLRNGTKKTKWYWKLK GIR +AIK AL+SSLH QR
Sbjct: 1   MEAKLATP-------RGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQR 60

Query: 61  LHLTCSTAM----NGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDF 120
             LTCSTA+      QLTP NG VSPLVI  SK  SKKNDLRLAKPLASLLRMPLRA DF
Sbjct: 61  RRLTCSTAITTTTTPQLTPTNGLVSPLVIHQSK-PSKKNDLRLAKPLASLLRMPLRAADF 120

Query: 121 IDYGNHMTPTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFD 180
           ID+GNHMTPTLSPR+NI+AVWR+LHG  DW+GLLDPLHPFLRRE+VKYGEFAQATYDAFD
Sbjct: 121 IDFGNHMTPTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFD 180

Query: 181 FDSLSEFCGSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDS 240
           FD LSEFCGSCRYNRHKLL ELGLAQNGYKVTKYIYALSPVDGP+WFESSKIGEVWSRDS
Sbjct: 181 FDPLSEFCGSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDS 240

Query: 241 NWMGFVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFL 300
           NWMG+VAVS+DEES+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR NKKVKVQRGFL
Sbjct: 241 NWMGYVAVSSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFL 300

Query: 301 TIYRSNDEQSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYE 360
           TIY+S DE S+FNKTSASEQVMEEL+RL++FFK+KGDREIS+TITGHSLGGALSLL AYE
Sbjct: 301 TIYKSKDEDSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTAYE 360

Query: 361 AGVSFP-DVHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVN 420
           AGV+FP DVH+SVVSFGAPR GNLAF+EKLNE+GVKTLRVV+RQDIVPKLPGL VNSIVN
Sbjct: 361 AGVTFPADVHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVN 420

Query: 421 KLSAITSKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF-------- 480
           KL A+T KLNWVYRHVG +L+M+M+MSPYLK +SDMSGSHNLEIYLHLVDGF        
Sbjct: 421 KLGAVTGKLNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGKFR 480

Query: 481 ------------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQP 519
                       GSDMLVEELRIPEFW QLPHKGL+KNR+GRWVKPGRNPED+PSPFSQP
Sbjct: 481 WNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQP 535

BLAST of Sgr019418 vs. ExPASy TrEMBL
Match: A0A0A0M111 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537320 PE=4 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 6.2e-251
Identity = 433/535 (80.93%), Postives = 473/535 (88.41%), Query Frame = 0

Query: 8   PRASTPTKRGVPLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQRLHLTCST 67
           P+ +TP  RG+P+++ TMLRNGTKKTKWYWKLK GI+ +AIK AL+SSLH QR  LTCST
Sbjct: 3   PKFATP--RGIPVKRTTMLRNGTKKTKWYWKLKLGIQFKAIKAALSSSLHNQRRRLTCST 62

Query: 68  AM---NGQLTPANGAVSPLVIQPSKSSSKKNDLRLAKPLASLLRMPLRAIDFIDYGNHMT 127
           A+     Q TP NG VSPLVI  SK SSKKNDLRLAKPLASLLRMPLRA DFID+GNHMT
Sbjct: 63  AITTTTPQHTPTNGLVSPLVIHQSK-SSKKNDLRLAKPLASLLRMPLRASDFIDFGNHMT 122

Query: 128 PTLSPRENITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSLSEFC 187
           PTLSPR+ I+AVWR+LHG  DW+GLLDPLHPFLRRE+VKYGEFAQATYDAFDFD LSEFC
Sbjct: 123 PTLSPRDKISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC 182

Query: 188 GSCRYNRHKLLQELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAV 247
           GSCRYNR KLL ELGLAQNGYKVTKYIYALSPVDGP+WFESSKIGEVWSRDSNWMGFVAV
Sbjct: 183 GSCRYNRQKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 242

Query: 248 SNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRENKKVKVQRGFLTIYRSNDE 307
           S+DEES+RIGRRDILV+WRGTVTPTEWYIDLKTKLKKIDR NKKVKVQRGFLTIY+S DE
Sbjct: 243 SSDEESDRIGRRDILVSWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKDE 302

Query: 308 QSRFNKTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVSFP-D 367
            S+FNKTSASEQVMEEL+RL++FFKQKGDREIS+TITGHSLGGALSLL AYEAGV+FP D
Sbjct: 303 DSKFNKTSASEQVMEELHRLIDFFKQKGDREISLTITGHSLGGALSLLTAYEAGVTFPAD 362

Query: 368 VHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAITSK 427
           VH+SVVSFGAPR GNLAF+EKLNE+GVK LRVV+ QDIVPKLPGL VNSIVNKLSA+T K
Sbjct: 363 VHVSVVSFGAPRVGNLAFREKLNEMGVKILRVVIGQDIVPKLPGLFVNSIVNKLSAVTGK 422

Query: 428 LNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF---------------- 487
           LNWVYRHVG +L+M+MFMSPYLK +SDMSGSHNLEIYLHLVDGF                
Sbjct: 423 LNWVYRHVGKELRMNMFMSPYLKKDSDMSGSHNLEIYLHLVDGFVTKKGKFRWNSRRDVA 482

Query: 488 ----GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGRNPEDVPSPFSQPSQI 519
               GSDMLVEELRIPEFW QLPHKGL+KNR+GRWVKPGRN ED+PSPFSQPSQ+
Sbjct: 483 LVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNVEDIPSPFSQPSQV 534

BLAST of Sgr019418 vs. TAIR 10
Match: AT1G30370.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 562.8 bits (1449), Expect = 2.9e-160
Identity = 294/521 (56.43%), Postives = 366/521 (70.25%), Query Frame = 0

Query: 19  PLRKVTMLRNGTKKTKWYWKLKFGIRLQAIKKALASSLHQQRLHLTCSTAMNGQLTPANG 78
           PLRK+       ++TK  W+LK     Q +K A  S   + + HL    +    L     
Sbjct: 9   PLRKLR-----RRRTKRVWRLK-----QKLKLAWKSIKIRVKSHLPGFLSTKKHLFHIKS 68

Query: 79  AVSPLVIQPSKSSSKKNDLRLAKPLASLLRMP-LRAIDFIDYGNHMTPTLSPRENITAVW 138
                 +        K      K LA LL++P   A DF+D G+ MTP  SPRE I+ +W
Sbjct: 69  RKEEQDLSQVAQRICKISNDSTKSLAFLLQLPKYSADDFLDRGDLMTPAASPREKISKMW 128

Query: 139 RELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRYNRHKLLQE 198
           RELHG  +W+ LLDPLHP+LRRE+ KYGEF ++ YD+ DFD LSEFCGS RYNR+KL +E
Sbjct: 129 RELHGSNNWENLLDPLHPWLRREVTKYGEFVESVYDSLDFDPLSEFCGSSRYNRNKLFEE 188

Query: 199 LGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGRRD 258
           LGL ++GYKVTKYIYA+S VD P+WF SS +GE WS+DSNWMGFVAVS D ES RIGRRD
Sbjct: 189 LGLTRHGYKVTKYIYAMSRVDVPQWFLSSALGETWSKDSNWMGFVAVSGDRESLRIGRRD 248

Query: 259 ILVAWRGTVTPTEWYIDLKTKLKKIDRENKK----VKVQRGFLTIYRSNDEQSRFNKTSA 318
           I+VAWRGTVTPTEW++DL+T ++  D E K     VKVQ GFL+IY S  E +R+NK SA
Sbjct: 249 IVVAWRGTVTPTEWFMDLRTSMEPFDCEGKHGKTVVKVQSGFLSIYNSKSELTRYNKESA 308

Query: 319 SEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVSFPDV--HISVVSF 378
           SEQ M+E+ RLV FFK +G+ E+S+TITGHSLGGAL+L+NAYEA    P +  +ISV+SF
Sbjct: 309 SEQTMDEVKRLVNFFKDRGE-EVSLTITGHSLGGALALMNAYEAARDVPALSGNISVISF 368

Query: 379 GAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSAITSKLNWVYRHV 438
           GAPR GNLAFKEKLN LGVK LRVV +QDIVPKLPG+V N ++NKL+ ITS+LNWVYRHV
Sbjct: 369 GAPRVGNLAFKEKLNSLGVKVLRVVNKQDIVPKLPGIVFNKVLNKLNPITSRLNWVYRHV 428

Query: 439 GTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF--------------------GSDM 498
           GTQLK+D+F SPY+K +SD+  +HNLE+YLH++DGF                     +DM
Sbjct: 429 GTQLKLDVFSSPYVKRDSDLGRAHNLEVYLHVLDGFHRKKSGFRVNARRDVASVNKSTDM 488

Query: 499 LVEELRIPEFWNQLPHKGLVKNRY-GRWVKPGRNPEDVPSP 512
           L++ LRIPEFW Q+ HKGL+ N+  GRWVKP R PED+PSP
Sbjct: 489 LLDHLRIPEFWYQVAHKGLILNKQTGRWVKPVRAPEDIPSP 518

BLAST of Sgr019418 vs. TAIR 10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 355.1 bits (910), Expect = 9.3e-98
Identity = 187/411 (45.50%), Postives = 258/411 (62.77%), Query Frame = 0

Query: 137 WRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRYNRHKLLQ 196
           WR++ G  DW GL+DP+ P LR EL++YGE AQA YDAFDFD  S++CG+ R+ R +   
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 197 ELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEES-ERIGR 256
            LG+  +GY+V +Y+YA S ++ P +F  S+  +VWS+++NWMG+VAVS+DE S  R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 257 RDILVAWRGTVTPTEWYIDLKTKLKKIDRENK------KVKVQRGFLTIYRSNDEQSRFN 316
           RDI +AWRGTVT  EW  DLK  LK +  ENK       VKV+ GFL +Y   D   +F 
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPV-TENKIRCPDPAVKVESGFLDLYTDKDTTCKFA 284

Query: 317 KTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVSFPD------ 376
           + SA EQ++ E+ RLVE      D ++SIT+TGHSLGGAL++L+AY+      +      
Sbjct: 285 RFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGK 344

Query: 377 -VHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVN-SIVNKLSAIT 436
            + ++V+++G PR GN+ F+E++ ELGVK +RVV   D+VPK PGL +N S  + L  I 
Sbjct: 345 VIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIA 404

Query: 437 SKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF-------------- 496
             L W Y HVG +L +D   SP+LK   D+S +HNLE  LHL+DG+              
Sbjct: 405 EGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLSSGRD 464

Query: 497 ------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGR-NPEDVPSP 512
                  SD L E L+IP FW Q  +KG+V+N  GRW++  R   ED  SP
Sbjct: 465 HALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of Sgr019418 vs. TAIR 10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 353.6 bits (906), Expect = 2.7e-97
Identity = 180/402 (44.78%), Postives = 247/402 (61.44%), Query Frame = 0

Query: 137 WRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRYNRHKLLQ 196
           WR++ G  DW GL+DP+ P LR EL++YGE AQA YDAFDFD  S +CGSCR+ R  L  
Sbjct: 89  WRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFD 148

Query: 197 ELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEESE--RIG 256
            LG+  +GY+V +Y+YA S ++ P +F  S+  +VWS+++NWMG+VAVS+D E+   R+G
Sbjct: 149 SLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLG 208

Query: 257 RRDILVAWRGTVTPTEWYIDLKTKLKKIDRE-----NKKVKVQRGFLTIYRSNDEQSRFN 316
           RRDI +AWRGTVT  EW  DLK  LK +        +  VK + GFL +Y   D    F+
Sbjct: 209 RRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFS 268

Query: 317 KTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAY---EAGVSFPD--- 376
           K SA EQV+ E+ RLVE +  +   E+SIT+TGHSLGGAL++L+AY   E GV+      
Sbjct: 269 KFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGK 328

Query: 377 -VHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNS-IVNKLSAIT 436
            + ++  ++G PR GN+ FKE++ +LGVK LRVV   D+V K PGL +N      L  + 
Sbjct: 329 VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLA 388

Query: 437 SKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF-------------- 496
             L W Y HVG  L +D   SP+LK   D+S +HNLE  LHL+DG+              
Sbjct: 389 GGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSSGRD 448

Query: 497 ------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGR 504
                  SD L +   +P +W Q  +KG+V+N  GRW++P R
Sbjct: 449 PALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDR 490

BLAST of Sgr019418 vs. TAIR 10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 329.7 bits (844), Expect = 4.2e-90
Identity = 175/427 (40.98%), Postives = 251/427 (58.78%), Query Frame = 0

Query: 132 NITAVWRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRYNR 191
           ++  +WRE+ G  +W+G LDP++  LRRE+++YGEFAQA YD+FDFD  S++CGSC+Y+ 
Sbjct: 86  SLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHP 145

Query: 192 HKLLQELGL-AQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEES 251
                 L L    GY +T+Y+YA S ++ P +F+ SK+  +WS+ +NWMGFVAV+ DEE 
Sbjct: 146 SDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEE 205

Query: 252 -ERIGRRDILVAWRGTVTPTEWYIDLKTKLKKID-RENKKVKVQRGFLTIYRSNDEQSRF 311
             R+GRRDI++AWRGTVT  EW  DLK  L   +  ++  +K++ GF  +Y   ++  +F
Sbjct: 206 VSRLGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKF 265

Query: 312 NKTSASEQVMEELYRLVEFF-KQKGDREISITITGHSLGGALSLLNAYEAGV----SFPD 371
           +  SA EQV+ E+ RL+E++  ++   + SIT+TGHSLG +L+L++AY+         P+
Sbjct: 266 SSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPE 325

Query: 372 ----VHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVNSIVNKLSA 431
               + I+V SF  PR GNL FKE+ +ELGVK LRVV   D VP +PG+  N        
Sbjct: 326 NNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKY 385

Query: 432 ITSKLN--WVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGF---------- 491
           +  K +  W Y HVG +L +D   SP+LK   D+  +HNLE  LHLVDG+          
Sbjct: 386 VEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKR 445

Query: 492 --------------GSDMLVEELRIPEFWNQLPHKGLVKNRYGRWVKPGR------NPED 515
                           D L  E  +P  W Q  +KG+VKN  G+WV P R       PED
Sbjct: 446 FCLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGPED 505

BLAST of Sgr019418 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 327.8 bits (839), Expect = 1.6e-89
Identity = 166/348 (47.70%), Postives = 231/348 (66.38%), Query Frame = 0

Query: 137 WRELHGLRDWDGLLDPLHPFLRRELVKYGEFAQATYDAFDFDSLSEFCGSCRYNRHKLLQ 196
           WR++ G  DW GL+DP+ P LR EL++YGE AQA YDAFDFD  S++CG+ R+ R +   
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 197 ELGLAQNGYKVTKYIYALSPVDGPEWFESSKIGEVWSRDSNWMGFVAVSNDEES-ERIGR 256
            LG+  +GY+V +Y+YA S ++ P +F  S+  +VWS+++NWMG+VAVS+DE S  R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 257 RDILVAWRGTVTPTEWYIDLKTKLKKIDRENK------KVKVQRGFLTIYRSNDEQSRFN 316
           RDI +AWRGTVT  EW  DLK  LK +  ENK       VKV+ GFL +Y   D   +F 
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPV-TENKIRCPDPAVKVESGFLDLYTDKDTTCKFA 284

Query: 317 KTSASEQVMEELYRLVEFFKQKGDREISITITGHSLGGALSLLNAYEAGVSFPD------ 376
           + SA EQ++ E+ RLVE      D ++SIT+TGHSLGGAL++L+AY+      +      
Sbjct: 285 RFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGK 344

Query: 377 -VHISVVSFGAPRAGNLAFKEKLNELGVKTLRVVVRQDIVPKLPGLVVN-SIVNKLSAIT 436
            + ++V+++G PR GN+ F+E++ ELGVK +RVV   D+VPK PGL +N S  + L  I 
Sbjct: 345 VIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIA 404

Query: 437 SKLNWVYRHVGTQLKMDMFMSPYLKHESDMSGSHNLEIYLHLVDGFGS 470
             L W Y HVG +L +D   SP+LK   D+S +HNLE  LHL+DG+ S
Sbjct: 405 EGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYVS 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022927629.13.7e-25882.16phospholipase A1-Igamma1, chloroplastic-like [Cucurbita moschata][more]
KAG6583869.14.9e-25882.16Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
KAG7019492.11.1e-25781.97Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023519232.13.2e-25781.78phospholipase A1-Igamma1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023001410.15.4e-25781.78phospholipase A1-Igamma1, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q3EBR61.3e-9645.50Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3... [more]
Q941F13.8e-9644.78Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4... [more]
Q9C8J65.9e-8940.98Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2... [more]
A2WY006.5e-6440.87Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica ... [more]
Q8S1D96.5e-6440.87Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonic... [more]
Match NameE-valueIdentityDescription
A0A6J1EIJ31.8e-25882.16phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KL462.6e-25781.78phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5A7UFJ94.6e-25480.48Phospholipase A1-Igamma1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3BBX34.6e-25480.48phospholipase A1-Igamma1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487991 ... [more]
A0A0A0M1116.2e-25180.93Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537320 PE... [more]
Match NameE-valueIdentityDescription
AT1G30370.12.9e-16056.43alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.29.3e-9845.50alpha/beta-Hydrolases superfamily protein [more]
AT1G06800.12.7e-9744.78alpha/beta-Hydrolases superfamily protein [more]
AT1G51440.14.2e-9040.98alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.11.6e-8947.70alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 272..292
NoneNo IPR availablePANTHERPTHR31403:SF13ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 469..516
NoneNo IPR availablePANTHERPTHR31403PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTICcoord: 19..467
coord: 469..516
NoneNo IPR availablePANTHERPTHR31403:SF13ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 19..467
NoneNo IPR availableCDDcd00519Lipase_3coord: 190..465
e-value: 6.71062E-47
score: 161.105
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 260..408
e-value: 2.3E-33
score: 115.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 136..511
e-value: 4.2E-119
score: 400.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 203..465

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr019418.1Sgr019418.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004620 phospholipase activity