Sgr019390 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr019390
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionPectinesterase
Locationtig00153347: 142997 .. 144812 (-)
RNA-Seq ExpressionSgr019390
SyntenySgr019390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCTACTCAGACCTTATGGCAAGGTCGACGAAACCGACCAAGTGAGGCTCGACGCTTCCCGGAGGACTCGGAAGAGGATTGCTGTCATTGCTTTGTCCTCTGTTCTTCTTGTGGGTGTTGTTCTTGCAGTTGTGTTTGGAACCAGAAATGGTTCCAAGAATGATGACAGTGACAATCACAATGCTGCTCTTTCAGCTTCTATCAAAGCAGTTTGTGATGTGACTTTGTATCCGGAGACCTGCTACAGTGCTTTTGGTCCTTTAACTAACTCGAGCCGCCTCGACCCTGGCCAGATCTGTAAGCTTTCGATTCAAGTGGCGCTCGGCGAACTGTCGCGAGCAGCTGACTACATTTTTGAGCATGCCATTACCAATTCAACTGATAATAAGACCATATTGGCTTTGAAAGATTGCCATGAACTTCTGGACTTGGCATTGGATCATCTGAGTATCTCATTGTCTGCTAGTGAGATTACATTGCTGAAAGCTGTGGATGATCTGAAAACATGGCTAACTTCAGCAGCTACTTACCAGCAGACTTGCATCGAAGGCCTCGAGGAAGTCGATCCGGCTTTGAAGAACATCATTGCAGATTACTTGAAGAACTCCACTGAATTGACCAGCAATGGGCTTGCCATTGTTTCCTTCTTTTCTAAACTTACGGATTCTCTCAATCTACGGCGATTGATGAGTTATGAAGATCACCAGAGCAGTGCAGATTGGGCGCACCCGATACTCCGAAAGCTAGCTCAAAGTGATCTCAGGAAGCAGGCAGACATTGTTGTAGCAAAAGACAGTTCGGGGAAGTACAAAACAATCACTGCAGCTCTCAAGGCTGTTCCTGACAAGAGCATGAAGAGAACTGTGATTTATGTGAAGAAGGGAAGTTATAAAGAGAATGTTGAAGTTGAGAAGAACAAGTGGAATGTGGTGATGATTGGTGATGGAATGAATGCTACCATTGTAACTGGCAGCCTCAACTTCATAGATGGAACTCCAACATTTGCATCTGCAACATTTGGTAAGAGAAAAAACAGATTCCTCTAAGCTTTTAACTAACTTGGTTTTCAAAGTCCATGACAGAGACATATTAAACAGGTTACAATCTGACCATCCATTTCCATGTGTTTTTTGTACAGCTGCAAAGGGCAAAGGCTTTATTGCCATTGACATGGGTTTCAGGAATACTGCTGGACCAAGCAAACACCAGGCGGTTGCCCTGATGTCGACCTCCGACCAATCAATCTTCTACCGTTGTCAAATGGATGCATACCAAGACACTCTCTACACACACTCCAACCGTCAATTCTACCGTGAATGCAAGGTTTACGGCACGGTTGATTTCATCTTTGGTAACTCAGCAGTGGTTCTACAAAACTGCTCCATTTTACCCAAGTTGCCTCTTTTAGGCCAACAAAACACAATCACTGCTCAGGGCCGGTTTGATCCCAATCAAAACACTGGCATTTCAATACAAGACTCTTCCATCCAACCATTTGGAGACTTGAAATCGACTCAAACTTACCTCGGTCGTCCATGGAAGAACTACTCGACTACTGTATATATGCAGAACAACTTGGGGAGCTTGATTAACCCCAATGGGTGGCTACCATGGGTAGGAACATCAGCACCAGACACCATATTCTATGCAGAATTCCAAAACTATGGCCCTGGTTCTTCCACAGCCAATAGAGTCAAATGGAAGGGTGTGAGGAACATTGACAACAAAACTGCCAAAAAATTCACAGTTTCCTCCTTCATTGGTAAGGATTGGATCTCAAAGGCACAAGTCCCCTTCCAATCCAGCCTCTGA

mRNA sequence

ATGTCTCTACTCAGACCTTATGGCAAGGTCGACGAAACCGACCAAGTGAGGCTCGACGCTTCCCGGAGGACTCGGAAGAGGATTGCTGTCATTGCTTTGTCCTCTGTTCTTCTTGTGGGTGTTGTTCTTGCAGTTGTGTTTGGAACCAGAAATGGTTCCAAGAATGATGACAGTGACAATCACAATGCTGCTCTTTCAGCTTCTATCAAAGCAGTTTGTGATGTGACTTTGTATCCGGAGACCTGCTACAGTGCTTTTGGTCCTTTAACTAACTCGAGCCGCCTCGACCCTGGCCAGATCTGTAAGCTTTCGATTCAAGTGGCGCTCGGCGAACTGTCGCGAGCAGCTGACTACATTTTTGAGCATGCCATTACCAATTCAACTGATAATAAGACCATATTGGCTTTGAAAGATTGCCATGAACTTCTGGACTTGGCATTGGATCATCTGAGTATCTCATTGTCTGCTAGTGAGATTACATTGCTGAAAGCTGTGGATGATCTGAAAACATGGCTAACTTCAGCAGCTACTTACCAGCAGACTTGCATCGAAGGCCTCGAGGAAGTCGATCCGGCTTTGAAGAACATCATTGCAGATTACTTGAAGAACTCCACTGAATTGACCAGCAATGGGCTTGCCATTGTTTCCTTCTTTTCTAAACTTACGGATTCTCTCAATCTACGGCGATTGATGAGTTATGAAGATCACCAGAGCAGTGCAGATTGGGCGCACCCGATACTCCGAAAGCTAGCTCAAAGTGATCTCAGGAAGCAGGCAGACATTGTTGTAGCAAAAGACAGTTCGGGGAAGTACAAAACAATCACTGCAGCTCTCAAGGCTGTTCCTGACAAGAGCATGAAGAGAACTGTGATTTATGTGAAGAAGGGAAGTTATAAAGAGAATGTTGAAGTTGAGAAGAACAAGTGGAATGTGGTGATGATTGGTGATGGAATGAATGCTACCATTGTAACTGGCAGCCTCAACTTCATAGATGGAACTCCAACATTTGCATCTGCAACATTTGCTGCAAAGGGCAAAGGCTTTATTGCCATTGACATGGGTTTCAGGAATACTGCTGGACCAAGCAAACACCAGGCGGTTGCCCTGATGTCGACCTCCGACCAATCAATCTTCTACCGTTGTCAAATGGATGCATACCAAGACACTCTCTACACACACTCCAACCGTCAATTCTACCGTGAATGCAAGGTTTACGGCACGGTTGATTTCATCTTTGGTAACTCAGCAGTGGTTCTACAAAACTGCTCCATTTTACCCAAGTTGCCTCTTTTAGGCCAACAAAACACAATCACTGCTCAGGGCCGGTTTGATCCCAATCAAAACACTGGCATTTCAATACAAGACTCTTCCATCCAACCATTTGGAGACTTGAAATCGACTCAAACTTACCTCGGTCGTCCATGGAAGAACTACTCGACTACTGTATATATGCAGAACAACTTGGGGAGCTTGATTAACCCCAATGGGTGGCTACCATGGGTAGGAACATCAGCACCAGACACCATATTCTATGCAGAATTCCAAAACTATGGCCCTGGTTCTTCCACAGCCAATAGAGTCAAATGGAAGGGTGTGAGGAACATTGACAACAAAACTGCCAAAAAATTCACAGTTTCCTCCTTCATTGGTAAGGATTGGATCTCAAAGGCACAAGTCCCCTTCCAATCCAGCCTCTGA

Coding sequence (CDS)

ATGTCTCTACTCAGACCTTATGGCAAGGTCGACGAAACCGACCAAGTGAGGCTCGACGCTTCCCGGAGGACTCGGAAGAGGATTGCTGTCATTGCTTTGTCCTCTGTTCTTCTTGTGGGTGTTGTTCTTGCAGTTGTGTTTGGAACCAGAAATGGTTCCAAGAATGATGACAGTGACAATCACAATGCTGCTCTTTCAGCTTCTATCAAAGCAGTTTGTGATGTGACTTTGTATCCGGAGACCTGCTACAGTGCTTTTGGTCCTTTAACTAACTCGAGCCGCCTCGACCCTGGCCAGATCTGTAAGCTTTCGATTCAAGTGGCGCTCGGCGAACTGTCGCGAGCAGCTGACTACATTTTTGAGCATGCCATTACCAATTCAACTGATAATAAGACCATATTGGCTTTGAAAGATTGCCATGAACTTCTGGACTTGGCATTGGATCATCTGAGTATCTCATTGTCTGCTAGTGAGATTACATTGCTGAAAGCTGTGGATGATCTGAAAACATGGCTAACTTCAGCAGCTACTTACCAGCAGACTTGCATCGAAGGCCTCGAGGAAGTCGATCCGGCTTTGAAGAACATCATTGCAGATTACTTGAAGAACTCCACTGAATTGACCAGCAATGGGCTTGCCATTGTTTCCTTCTTTTCTAAACTTACGGATTCTCTCAATCTACGGCGATTGATGAGTTATGAAGATCACCAGAGCAGTGCAGATTGGGCGCACCCGATACTCCGAAAGCTAGCTCAAAGTGATCTCAGGAAGCAGGCAGACATTGTTGTAGCAAAAGACAGTTCGGGGAAGTACAAAACAATCACTGCAGCTCTCAAGGCTGTTCCTGACAAGAGCATGAAGAGAACTGTGATTTATGTGAAGAAGGGAAGTTATAAAGAGAATGTTGAAGTTGAGAAGAACAAGTGGAATGTGGTGATGATTGGTGATGGAATGAATGCTACCATTGTAACTGGCAGCCTCAACTTCATAGATGGAACTCCAACATTTGCATCTGCAACATTTGCTGCAAAGGGCAAAGGCTTTATTGCCATTGACATGGGTTTCAGGAATACTGCTGGACCAAGCAAACACCAGGCGGTTGCCCTGATGTCGACCTCCGACCAATCAATCTTCTACCGTTGTCAAATGGATGCATACCAAGACACTCTCTACACACACTCCAACCGTCAATTCTACCGTGAATGCAAGGTTTACGGCACGGTTGATTTCATCTTTGGTAACTCAGCAGTGGTTCTACAAAACTGCTCCATTTTACCCAAGTTGCCTCTTTTAGGCCAACAAAACACAATCACTGCTCAGGGCCGGTTTGATCCCAATCAAAACACTGGCATTTCAATACAAGACTCTTCCATCCAACCATTTGGAGACTTGAAATCGACTCAAACTTACCTCGGTCGTCCATGGAAGAACTACTCGACTACTGTATATATGCAGAACAACTTGGGGAGCTTGATTAACCCCAATGGGTGGCTACCATGGGTAGGAACATCAGCACCAGACACCATATTCTATGCAGAATTCCAAAACTATGGCCCTGGTTCTTCCACAGCCAATAGAGTCAAATGGAAGGGTGTGAGGAACATTGACAACAAAACTGCCAAAAAATTCACAGTTTCCTCCTTCATTGGTAAGGATTGGATCTCAAAGGCACAAGTCCCCTTCCAATCCAGCCTCTGA

Protein sequence

MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKNDDSDNHNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIFEHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQQTCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSSADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSYKENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVVLQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNYSTTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNKTAKKFTVSSFIGKDWISKAQVPFQSSL
Homology
BLAST of Sgr019390 vs. NCBI nr
Match: XP_022140040.1 (putative pectinesterase/pectinesterase inhibitor 24 [Momordica charantia])

HSP 1 Score: 986.9 bits (2550), Expect = 7.4e-284
Identity = 500/568 (88.03%), Postives = 534/568 (94.01%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKND--DS 60
           MSLLRPYGKVDETDQ+RLDASRRTRKRIAVI LSSV+LV VV+AVVFGTRNGSK+D   +
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60

Query: 61  DNHNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADY 120
            N N+ALS+SIKAVCDVTLYPETC  AFGPL+NSSRLDPGQICKLS+Q+ALGELSRAADY
Sbjct: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120

Query: 121 IFEHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATY 180
           +F+HAITNSTDN+T LAL+ CHELLDLALDHL+ISLS++EITLLKAVDDLKTWLTSAATY
Sbjct: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180

Query: 181 QQTCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQS 240
           QQTCIEGLEEVDP L+N IA+YLKNSTELTSNGLAIVSFFSK+TDSLNLRRLMSYE+HQS
Sbjct: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240

Query: 241 SADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSY 300
           SADWA PILRKLAQ DLRKQADIVVAKD SGKYKTI+AALKAVPDKS K TVIYVKKG+Y
Sbjct: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300

Query: 301 KENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNT 360
           KENVEV KNKWNVVMIGDGMNAT VT  LNFIDGTPTF+SATFAAKGKGFIAIDMGF+N 
Sbjct: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRC+MDAYQDTLY HSNRQFYREC V GTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420

Query: 421 LQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNY 480
           LQNC+ILPKLPLLGQ+NTITAQGRFDPNQNTGISIQDSSI P+ +LK+T+TYLGRPWKNY
Sbjct: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480

Query: 481 STTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNK 540
           STTV+MQN LGSLI P GWLPWVGTSAPDTIFYAEFQNYGPGSSTA RVKWKGVRNIDNK
Sbjct: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540

Query: 541 TAKKFTVSSFI-GKDWISKAQVPFQSSL 566
           TAKKFTVSSFI GKDWISKAQVPF+S L
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 568

BLAST of Sgr019390 vs. NCBI nr
Match: XP_038895610.1 (putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida])

HSP 1 Score: 983.4 bits (2541), Expect = 8.1e-283
Identity = 495/567 (87.30%), Postives = 537/567 (94.71%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGS-KNDDSD 60
           MSLLRPYGKVDETDQ+RLDASRRTRKRIAVI LSSV+LVGVV+AVVFGTRN S  NDDS+
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60

Query: 61  NHNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYI 120
           N + ALS SIKAVCDVTLYP TC +AFGPL NSS LDP QI KLS+Q+ALGELSR ADYI
Sbjct: 61  NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120

Query: 121 FEHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQ 180
           F HAITNSTDNKTILALK+CHELLDLALDHL+ISLS+S+ITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSS 240
           QTCI+GL EVDPAL N+IA+YLKNSTELTSNGLAIVSFFSKLTDSL+LRRLMSYEDHQS+
Sbjct: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240

Query: 241 ADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSYK 300
            DWA PILRKLAQ D+RKQADIVVAKDSSGKYKTITAAL+AVP+KS KRTVIYVKKG+YK
Sbjct: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNTA 360
           ENVEV KNKWNVV++GDGM ATIVTGSLN IDGTPTF++ATFAAKGKGFIAIDMGFRNTA
Sbjct: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GP+KHQAVALMSTSDQSIFYRCQMDAYQDTLY+HSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNYS 480
           QNC+I P+LPLLGQ+NTITAQGRFDPNQNTGISIQDSSIQP+ +LK+T+TYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480

Query: 481 TTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNKT 540
           TTV+MQ+NLGSLI+P+GWLPWVGTSAPDTIFYAEFQNYGPGSSTA RVKW GV+NID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540

Query: 541 AKKFTVSSFI-GKDWISKAQVPFQSSL 566
           AKKFTVSSFI GKDWISKAQVPF++S+
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 567

BLAST of Sgr019390 vs. NCBI nr
Match: XP_023000829.1 (probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima])

HSP 1 Score: 964.5 bits (2492), Expect = 3.9e-277
Identity = 483/566 (85.34%), Postives = 521/566 (92.05%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKNDDSDN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VI LSSV+LVGVV+AVVFGTRN SKND+SDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIF 120
           HN ALS SIKAVCD TLYP+TC +AFGPL NSS LDPGQI K S+Q+AL ELSR ADY+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQQ 180
           EHAITNSTDNKTILAL+ CHELLDLA+DHL+ SLS+SEIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSSA 240
           TCI+GLEEVD AL N+IA+YLKNSTE+TSNGLAIVSFFSKLTDS +LRRLMSYED  S+ 
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSYKE 300
           DWA PILRKLAQ DLRKQADIVVAKD SGKYKTIT ALKAV DKS KRTVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNTAG 360
           NVEV KNKWNVVMIGDGM +T VTG LNFIDGTPTF++ATFAAKGKGFIAIDMGF NTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQM+AYQDTLY HSNRQFYR+CKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNYST 480
           NC I PKLPLLGQ+NTITAQGRFDPNQ+TGISIQ SSIQPF +LK+TQTYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYST 480

Query: 481 TVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNKTA 540
           TV+MQNN GSLI+P GWLPWVGTSAPDTIFYAEFQN GPGSSTANRVKWKG++NID KTA
Sbjct: 481 TVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFI-GKDWISKAQVPFQSSL 566
           KKFTVSSFI GKDWISKAQVPF ++L
Sbjct: 541 KKFTVSSFIDGKDWISKAQVPFNATL 566

BLAST of Sgr019390 vs. NCBI nr
Match: KAG6583848.1 (putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 961.8 bits (2485), Expect = 2.5e-276
Identity = 481/566 (84.98%), Postives = 521/566 (92.05%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKNDDSDN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VI LSSV+LVGVV+AVVFGTRN SKND+SDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIF 120
           HN ALS SIKAVCD TLYP+TC +AFG L NSS LDPGQI K S+Q+ALGELSR ADY+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQQ 180
           EHAITNSTDNKTILAL+ CHELLDLA+DHL+ SLS+SEIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAMDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSSA 240
           TCI+GLEEVD AL N+IA+YLKNSTE+TSNGLAIVSFFSKLTDS +LRRLMSYED  S+ 
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSYKE 300
           DWA PILRKLAQ DLRKQADIVVAKD SGKYKTIT AL AVPDKS KRTVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNTAG 360
           NVEV KNKWNVVMIGDGM +T VTG LNFIDGTPTF++ATFAAKGKGFIAIDMGF NTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQM+AYQDTLY HSNRQFYR+CKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNYST 480
           NC I PKLPLLGQ+NTITAQGRFDPNQ+TGISIQ SSIQPF +LK+T+TYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNKTA 540
           TV+MQN+ GSLI+P GWLPWVGTSAPDTIFYAEFQN GPGSSTANRVKWKG++NID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFI-GKDWISKAQVPFQSSL 566
           KKFTVSSFI GKDWISKAQVPF ++L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of Sgr019390 vs. NCBI nr
Match: XP_022927225.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata])

HSP 1 Score: 961.8 bits (2485), Expect = 2.5e-276
Identity = 481/566 (84.98%), Postives = 521/566 (92.05%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKNDDSDN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VI LSSV+LVGVV+AVVFGTRN SKND+SDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIF 120
           HN ALS SIKAVCD TLYP+TC +AFG L NSS LDPGQI K S+Q+ALGELSR ADY+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQQ 180
           EHAITNSTDNKTILAL+ CHELLDLA+DHL+ SLS+SEIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSSA 240
           TCI+GLEEVD AL N+IA+YLKNSTE+TSNGLAIVSFFSKLTDS +LRRLMSYED  S+ 
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSYKE 300
           DWA PILRKLAQ DLRKQADIVVAKD SGKYKTIT AL AVPDKS KRTVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNTAG 360
           NVEV KNKWNVVMIGDGM +T VTG LNFIDGTPTF++ATFAAKGKGFIAIDMGF NTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQM+AYQDTLY HSNRQFYR+CKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNYST 480
           NC I PKLPLLGQ+NTITAQGRFDPNQ+TGISIQ SSIQPF +LK+T+TYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNKTA 540
           TV+MQN+ GSLI+P GWLPWVGTSAPDTIFYAEFQN GPGSSTANRVKWKG++NID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFI-GKDWISKAQVPFQSSL 566
           KKFTVSSFI GKDWISKAQVPF ++L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of Sgr019390 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.5e-170
Identity = 314/569 (55.18%), Postives = 410/569 (72.06%), Query Frame = 0

Query: 6   PYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKND---DSDNHN 65
           PYGKVDE + VRL+A R+TRK IA+IA+S V+L G+V+  VFGT    K+    +++N+ 
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  AALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIFEH 125
            ++S S+KAVCDVTL+ E C+   G   N+S L+P ++ + ++++ + E+S+A      +
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI-----N 123

Query: 126 AITNST-DNKTILALKDCHELLDLALDHLSISLSAS---EITLLKAVDDLKTWLTSAATY 185
           A ++S  D K  + +  C ELLDL +D+L+ +L++S   ++T+ + VDDL+TWL+SA TY
Sbjct: 124 AFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTY 183

Query: 186 QQTCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNL-RRLMSYEDHQ 245
           Q+TC+E L    P ++     +LKNSTELTSN LAI+++  K+ DS  L RRL++  D +
Sbjct: 184 QRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVE 243

Query: 246 SSADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGS 305
                 H   R L  +DLRK ADIVVAKD SGKY+TI  AL+ VP+KS KRT+IYVKKG 
Sbjct: 244 VD---FHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGV 303

Query: 306 YKENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRN 365
           Y ENV+VEK  WNV+++GDG + +IV+G LN IDGTPTF +ATFA  GKGF+A DMGF N
Sbjct: 304 YFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFIN 363

Query: 366 TAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAV 425
           TAGPSKHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYREC + GTVDFIFGNSA 
Sbjct: 364 TAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSAS 423

Query: 426 VLQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKN 485
           VLQ+C ILP+ P+ GQQNTITAQGR DPN NTGISI   +I P GDL    T+LGRPWKN
Sbjct: 424 VLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKN 483

Query: 486 YSTTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDN 545
           +STTV M + L   I+  GWLPW G SAPDTIFY E++N GPG+ST NRVKWKG+R +  
Sbjct: 484 FSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLST 543

Query: 546 KTAKKFTVSSFI-GKDWISKAQVPFQSSL 566
           K A +FTV  FI G  W+   +VPF+S L
Sbjct: 544 KEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of Sgr019390 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 8.5e-166
Identity = 304/568 (53.52%), Postives = 406/568 (71.48%), Query Frame = 0

Query: 7   YGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGT--RNGSKNDDSDNHNAA 66
           YG++DE +Q +L+ASR+T+KRIA+IA+SS++LV +V+  V GT  R+ SK   ++N+   
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIF--EH 126
           +S S+KA+CDVTL+ E C+   G   N+SR  P ++ K +++V + ELS+  D     EH
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEH 123

Query: 127 AITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQQTC 186
                 DN T  A+  C EL+ LA+D L+ ++++S    LK  DDL+TWL+S  TYQ+TC
Sbjct: 124 -----MDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETC 183

Query: 187 IEGLEEVD-PALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSSAD 246
           ++ L E + P+L     ++LKNSTE+TSN LAI+++  K+ D++  RR    E   +   
Sbjct: 184 MDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVV 243

Query: 247 WAH-PIL---RKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGS 306
            A  P++   R L   DL+K+A IVVAKD SGKY+TI  AL  V +K+ K T+IYVKKG 
Sbjct: 244 VADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGV 303

Query: 307 YKENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRN 366
           Y ENV VEK KWNVVM+GDG + TIV+  LNFIDGTPTF +ATFA  GKGF+A DMGF N
Sbjct: 304 YLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFIN 363

Query: 367 TAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAV 426
           TAGP+KHQAVALM ++D S+FY+C MDA+QDT+Y H+ RQFYR+C + GTVDFIFGN+AV
Sbjct: 364 TAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAV 423

Query: 427 VLQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKN 486
           V Q C ILP+ P+ GQQNTITAQGR DPNQNTGISI + +I+P  +L   QT+LGRPWK+
Sbjct: 424 VFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKD 483

Query: 487 YSTTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVR-NID 546
           +STTV M++ +   INP GWLPW G +APDTIFYAE+ N GPG+ST NRVKW+G++ ++ 
Sbjct: 484 FSTTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLT 543

Query: 547 NKTAKKFTVSSFI-GKDWISKAQVPFQS 564
            K A KFTV  FI G +W+   +VPF S
Sbjct: 544 KKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of Sgr019390 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 7.5e-138
Identity = 275/583 (47.17%), Postives = 382/583 (65.52%), Query Frame = 0

Query: 7   YGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFG----TRNGSKNDDSDN-- 66
           YGKV+E +Q   +  ++TRKR+ +IA+SS++L+ V++A V G     RN   +  SD+  
Sbjct: 10  YGKVNELEQQAYE--KKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVP 69

Query: 67  -HNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYI 126
               + +AS+KAVCD T YP +C+S+   L  S+  DP  + KLS++VA+ ELS     +
Sbjct: 70  QTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKL 129

Query: 127 FEHAITNSTDNKTILALKDCHELLDLALDHLSISLS-----ASEITLLKAVDDLKTWLTS 186
             +A     D +   A+  C  +   ALD L+ S+S     A  I    +V +++TWL++
Sbjct: 130 RANA---EQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSA 189

Query: 187 AATYQQTCIEGLEEVDP-----ALKNIIADYLKNSTELTSNGLAIVS----FFSKLTDSL 246
           A T Q TC++ + E++      AL+  I   ++NSTE  SN LAIV+      S+    +
Sbjct: 190 ALTDQDTCLDAVGELNSTAARGALQE-IETAMRNSTEFASNSLAIVTKILGLLSRFETPI 249

Query: 247 NLRRLMSYEDHQSSADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKS 306
           + RRL+ + +   +A+      R L + +     D VVAKD SG++KTI  ALK V  KS
Sbjct: 250 HHRRLLGFPEWLGAAE-----RRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKS 309

Query: 307 MKRTVIYVKKGSYKENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKG 366
            +R  +YVK+G Y EN++++KN WNV++ GDG + T V GS NF+DGTPTF +ATFA KG
Sbjct: 310 EERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKG 369

Query: 367 KGFIAIDMGFRNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVY 426
           KGFIA D+GF N AG SKHQAVAL S SD+S+F+RC  D +QDTLY HSNRQFYR+C + 
Sbjct: 370 KGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDIT 429

Query: 427 GTVDFIFGNSAVVLQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLK 486
           GT+DFIFGN+AVV Q+C I+P+ PL  Q NTITAQG+ DPNQNTGI IQ S+I PFG+  
Sbjct: 430 GTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNL 489

Query: 487 STQTYLGRPWKNYSTTVYMQNNLGSLINPNGWLPWV-GTSAPDTIFYAEFQNYGPGSSTA 546
           +  TYLGRPWK++STTV MQ+++G+L+NP GW+ WV     P TIFYAE+QN GPG+  +
Sbjct: 490 TAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVS 549

Query: 547 NRVKWKGVR-NIDNKTAKKFTVSSFI-GKDWISKAQVPFQSSL 566
            RVKW G +  I ++ A++FTV SFI G +W+  A V F S+L
Sbjct: 550 QRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

BLAST of Sgr019390 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 7.2e-125
Identity = 255/583 (43.74%), Postives = 364/583 (62.44%), Query Frame = 0

Query: 7   YGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGT---RNGSKNDDSDNHNA 66
           YGKVDE   + L   ++TRKR+ ++++S V+L+ V++A V  T   +N +++  S     
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  ALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIFEHA 126
             S S+KA+C VT +PE+C S+   L +S+  DP  + KLS++V + EL   +D + E  
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKL 129

Query: 127 ITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLK------AVDDLKTWLTSAAT 186
              + D +   AL+ C +L++ ALD L+ ++SA +    K       ++DLKTWL++  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIEGLEEV--------DPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNL-- 246
             +TC + L+E+        +  +   +   +  STE TSN LAIVS        L +  
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 ---RRLMSYEDHQSSADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDK 306
              RRLMS+  HQ S D+     R+L Q+   K  D+ VA D +G   T+  A+  VP K
Sbjct: 250 HRRRRLMSHH-HQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SMKRTVIYVKKGSYKENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAK 366
           S+K  VIYVK G+Y ENV ++K+KWNV++ GDG   TI++GS NF+DGTPT+ +ATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFRNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKV 426
           GKGFI  D+G  NTAG +KHQAVA  S SD S++Y+C  D +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 YGTVDFIFGNSAVVLQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDL 486
            GT+DFIFG++AVV Q C I+P+ PL  Q NTITAQG+ DPNQ++G+SIQ  +I   G++
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNV 489

Query: 487 KSTQTYLGRPWKNYSTTVYMQNNLGSLINPNGWLPWV-GTSAPDTIFYAEFQNYGPGSST 546
               TYLGRPWK +STTV M+  +G+++ P+GW+ WV G   P +I Y E++N GPGS  
Sbjct: 490 -IAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 ANRVKWKGVRNI-DNKTAKKFTVSSFI-GKDWISKAQVPFQSS 565
             RVKW G + +  +  A KFTV++ + G DWI    V  Q S
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGVINQLS 586

BLAST of Sgr019390 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 5.6e-109
Identity = 247/585 (42.22%), Postives = 345/585 (58.97%), Query Frame = 0

Query: 9   KVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKNDDSDNHNAALSAS 68
           +V++    ++D  +R +K+I +  ++SVLLV  V+ VV G ++ SKN D      A+S+S
Sbjct: 3   RVEDFFSKQIDFCKR-KKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSS 62

Query: 69  ----IKAVCDVTLYPETCYSAFGPLTNSSRLDPGQ--ICKLSIQVALGELSRAADYIFEH 128
               +K+ C  TL+PE CYSA   +++ S+    Q  + +LS+ + +  + R    + E 
Sbjct: 63  AHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKEL 122

Query: 129 AIT-NSTDNKTILALKDCHELLDLALDHLSISLSASEI-----TLLKAVDDLKTWLTSAA 188
             T      +  +AL DC E +D  LD L  ++   E+     +L + V+DLKT ++SA 
Sbjct: 123 IKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAI 182

Query: 189 TYQQTCIEGL--EEVDPALKNIIADYLKNSTELTSNGLAIVSFFS--------KLTDSLN 248
           T Q+TC++G   +E D  ++ ++    K+  ++ SN LA++   +        KL+   N
Sbjct: 183 TNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPAN 242

Query: 249 LRRLMSYEDHQSSADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSM 308
            R+L+  ED+    +W     R+L QS      D+VVA D SG YKT++ A++  P+KS 
Sbjct: 243 NRKLV--EDNGEWPEWLSAGDRRLLQSS-TVTPDVVVAADGSGDYKTVSEAVRKAPEKSS 302

Query: 309 KRTVIYVKKGSYKENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGK 368
           KR VI +K G Y+ENV+V K K N++ +GDG + TI+T S N  DG+ TF SAT      
Sbjct: 303 KRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAG 362

Query: 369 GFIAIDMGFRNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYG 428
             +A D+ F+NTAG SKHQAVAL   SD S FYRC M AYQDTLY HSNRQF+ +C V G
Sbjct: 363 KVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAG 422

Query: 429 TVDFIFGNSAVVLQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDL-- 488
           TVDFIFGN A V Q+C I  + P  GQ+N +TAQGR DPNQNTGI IQ   I    DL  
Sbjct: 423 TVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRP 482

Query: 489 --KSTQTYLGRPWKNYSTTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSS 548
             KS  TYLGRPWK YS TV MQ+++  +I P GW  W G  A DT+FY E+ N G G+ 
Sbjct: 483 VQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAP 542

Query: 549 TANRVKWKGVRNIDNKT-AKKFTVSSFI-GKDWISKAQVPFQSSL 566
           T+ RVKWKG + I + T A+ +T   FI G  W+S    PF   L
Sbjct: 543 TSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of Sgr019390 vs. ExPASy TrEMBL
Match: A0A6J1CDY3 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010791 PE=3 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 3.6e-284
Identity = 500/568 (88.03%), Postives = 534/568 (94.01%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKND--DS 60
           MSLLRPYGKVDETDQ+RLDASRRTRKRIAVI LSSV+LV VV+AVVFGTRNGSK+D   +
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60

Query: 61  DNHNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADY 120
            N N+ALS+SIKAVCDVTLYPETC  AFGPL+NSSRLDPGQICKLS+Q+ALGELSRAADY
Sbjct: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120

Query: 121 IFEHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATY 180
           +F+HAITNSTDN+T LAL+ CHELLDLALDHL+ISLS++EITLLKAVDDLKTWLTSAATY
Sbjct: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180

Query: 181 QQTCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQS 240
           QQTCIEGLEEVDP L+N IA+YLKNSTELTSNGLAIVSFFSK+TDSLNLRRLMSYE+HQS
Sbjct: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240

Query: 241 SADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSY 300
           SADWA PILRKLAQ DLRKQADIVVAKD SGKYKTI+AALKAVPDKS K TVIYVKKG+Y
Sbjct: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300

Query: 301 KENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNT 360
           KENVEV KNKWNVVMIGDGMNAT VT  LNFIDGTPTF+SATFAAKGKGFIAIDMGF+N 
Sbjct: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRC+MDAYQDTLY HSNRQFYREC V GTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420

Query: 421 LQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNY 480
           LQNC+ILPKLPLLGQ+NTITAQGRFDPNQNTGISIQDSSI P+ +LK+T+TYLGRPWKNY
Sbjct: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480

Query: 481 STTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNK 540
           STTV+MQN LGSLI P GWLPWVGTSAPDTIFYAEFQNYGPGSSTA RVKWKGVRNIDNK
Sbjct: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540

Query: 541 TAKKFTVSSFI-GKDWISKAQVPFQSSL 566
           TAKKFTVSSFI GKDWISKAQVPF+S L
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 568

BLAST of Sgr019390 vs. ExPASy TrEMBL
Match: A0A6J1KL23 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.9e-277
Identity = 483/566 (85.34%), Postives = 521/566 (92.05%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKNDDSDN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VI LSSV+LVGVV+AVVFGTRN SKND+SDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIF 120
           HN ALS SIKAVCD TLYP+TC +AFGPL NSS LDPGQI K S+Q+AL ELSR ADY+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQQ 180
           EHAITNSTDNKTILAL+ CHELLDLA+DHL+ SLS+SEIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSSA 240
           TCI+GLEEVD AL N+IA+YLKNSTE+TSNGLAIVSFFSKLTDS +LRRLMSYED  S+ 
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSYKE 300
           DWA PILRKLAQ DLRKQADIVVAKD SGKYKTIT ALKAV DKS KRTVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNTAG 360
           NVEV KNKWNVVMIGDGM +T VTG LNFIDGTPTF++ATFAAKGKGFIAIDMGF NTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQM+AYQDTLY HSNRQFYR+CKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNYST 480
           NC I PKLPLLGQ+NTITAQGRFDPNQ+TGISIQ SSIQPF +LK+TQTYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYST 480

Query: 481 TVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNKTA 540
           TV+MQNN GSLI+P GWLPWVGTSAPDTIFYAEFQN GPGSSTANRVKWKG++NID KTA
Sbjct: 481 TVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFI-GKDWISKAQVPFQSSL 566
           KKFTVSSFI GKDWISKAQVPF ++L
Sbjct: 541 KKFTVSSFIDGKDWISKAQVPFNATL 566

BLAST of Sgr019390 vs. ExPASy TrEMBL
Match: A0A6J1ENB0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 1.2e-276
Identity = 481/566 (84.98%), Postives = 521/566 (92.05%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKNDDSDN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VI LSSV+LVGVV+AVVFGTRN SKND+SDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIF 120
           HN ALS SIKAVCD TLYP+TC +AFG L NSS LDPGQI K S+Q+ALGELSR ADY+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQQ 180
           EHAITNSTDNKTILAL+ CHELLDLA+DHL+ SLS+SEIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSSA 240
           TCI+GLEEVD AL N+IA+YLKNSTE+TSNGLAIVSFFSKLTDS +LRRLMSYED  S+ 
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSYKE 300
           DWA PILRKLAQ DLRKQADIVVAKD SGKYKTIT AL AVPDKS KRTVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNTAG 360
           NVEV KNKWNVVMIGDGM +T VTG LNFIDGTPTF++ATFAAKGKGFIAIDMGF NTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQM+AYQDTLY HSNRQFYR+CKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNYST 480
           NC I PKLPLLGQ+NTITAQGRFDPNQ+TGISIQ SSIQPF +LK+T+TYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNKTA 540
           TV+MQN+ GSLI+P GWLPWVGTSAPDTIFYAEFQN GPGSSTANRVKWKG++NID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFI-GKDWISKAQVPFQSSL 566
           KKFTVSSFI GKDWISKAQVPF ++L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of Sgr019390 vs. ExPASy TrEMBL
Match: A0A5A7VDG1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G00770 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.0e-275
Identity = 480/567 (84.66%), Postives = 529/567 (93.30%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGS-KNDDSD 60
           MS LRPYGKVDETDQ+RLDASRRTR+RI VI LSSV+LV VV+AVVFGTRN S  NDDS+
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NHNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYI 120
           N N +LSASIKAVCDVTLYP+TC  AFGP  NSS LDPGQI KL++Q+ALGELS+ AD+I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FEHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQ 180
           F+HAITN+TD+KTILALK+CHELLDLALDHL+I+LS+S+ITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSS 240
           QTCI+GL EVDPAL ++ A++LKNSTELTSNGLAIVSFFSKLTDSL+LRRLMSYED QS+
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 ADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSYK 300
            DWA PILRKLAQSD+RKQADIVVAKDSSGKYKTIT ALKAVPDKS KRTVIYVKKG+YK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNTA 360
           E VEV KNKWNVVM+GDGMNATIV+GSLN IDGTPTF++A+FAAKGKGFIAIDMGF NTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCQM+AYQDTLY HSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNYS 480
           QNC+I P+LPLLGQ+NTITAQGRFDPNQNTGISIQ S IQPF +LK+T+TYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNKT 540
           TTV+MQ+NLGSLI+P+GWLPWVGTSAPDTIFYAEFQNYG GSST+ RVKWKGV+NID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFI-GKDWISKAQVPFQSSL 566
           AKKFTVSSFI GKDWISKAQVPF ++L
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of Sgr019390 vs. ExPASy TrEMBL
Match: A0A1S3BDA4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.0e-275
Identity = 480/567 (84.66%), Postives = 529/567 (93.30%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGS-KNDDSD 60
           MS LRPYGKVDETDQ+RLDASRRTR+RI VI LSSV+LV VV+AVVFGTRN S  NDDS+
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NHNAALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYI 120
           N N +LSASIKAVCDVTLYP+TC  AFGP  NSS LDPGQI KL++Q+ALGELS+ AD+I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FEHAITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQ 180
           F+HAITN+TD+KTILALK+CHELLDLALDHL+I+LS+S+ITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSS 240
           QTCI+GL EVDPAL ++ A++LKNSTELTSNGLAIVSFFSKLTDSL+LRRLMSYED QS+
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 ADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSYK 300
            DWA PILRKLAQSD+RKQADIVVAKDSSGKYKTIT ALKAVPDKS KRTVIYVKKG+YK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNTA 360
           E VEV KNKWNVVM+GDGMNATIV+GSLN IDGTPTF++A+FAAKGKGFIAIDMGF NTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCQM+AYQDTLY HSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKNYS 480
           QNC+I P+LPLLGQ+NTITAQGRFDPNQNTGISIQ S IQPF +LK+T+TYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDNKT 540
           TTV+MQ+NLGSLI+P+GWLPWVGTSAPDTIFYAEFQNYG GSST+ RVKWKGV+NID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFI-GKDWISKAQVPFQSSL 566
           AKKFTVSSFI GKDWISKAQVPF ++L
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of Sgr019390 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 600.9 bits (1548), Expect = 1.1e-171
Identity = 314/569 (55.18%), Postives = 410/569 (72.06%), Query Frame = 0

Query: 6   PYGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKND---DSDNHN 65
           PYGKVDE + VRL+A R+TRK IA+IA+S V+L G+V+  VFGT    K+    +++N+ 
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  AALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIFEH 125
            ++S S+KAVCDVTL+ E C+   G   N+S L+P ++ + ++++ + E+S+A      +
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI-----N 123

Query: 126 AITNST-DNKTILALKDCHELLDLALDHLSISLSAS---EITLLKAVDDLKTWLTSAATY 185
           A ++S  D K  + +  C ELLDL +D+L+ +L++S   ++T+ + VDDL+TWL+SA TY
Sbjct: 124 AFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTY 183

Query: 186 QQTCIEGLEEVDPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNL-RRLMSYEDHQ 245
           Q+TC+E L    P ++     +LKNSTELTSN LAI+++  K+ DS  L RRL++  D +
Sbjct: 184 QRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVE 243

Query: 246 SSADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGS 305
                 H   R L  +DLRK ADIVVAKD SGKY+TI  AL+ VP+KS KRT+IYVKKG 
Sbjct: 244 VD---FHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGV 303

Query: 306 YKENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRN 365
           Y ENV+VEK  WNV+++GDG + +IV+G LN IDGTPTF +ATFA  GKGF+A DMGF N
Sbjct: 304 YFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFIN 363

Query: 366 TAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAV 425
           TAGPSKHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYREC + GTVDFIFGNSA 
Sbjct: 364 TAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSAS 423

Query: 426 VLQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKN 485
           VLQ+C ILP+ P+ GQQNTITAQGR DPN NTGISI   +I P GDL    T+LGRPWKN
Sbjct: 424 VLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKN 483

Query: 486 YSTTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDN 545
           +STTV M + L   I+  GWLPW G SAPDTIFY E++N GPG+ST NRVKWKG+R +  
Sbjct: 484 FSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLST 543

Query: 546 KTAKKFTVSSFI-GKDWISKAQVPFQSSL 566
           K A +FTV  FI G  W+   +VPF+S L
Sbjct: 544 KEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of Sgr019390 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 585.1 bits (1507), Expect = 6.0e-167
Identity = 304/568 (53.52%), Postives = 406/568 (71.48%), Query Frame = 0

Query: 7   YGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGT--RNGSKNDDSDNHNAA 66
           YG++DE +Q +L+ASR+T+KRIA+IA+SS++LV +V+  V GT  R+ SK   ++N+   
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIF--EH 126
           +S S+KA+CDVTL+ E C+   G   N+SR  P ++ K +++V + ELS+  D     EH
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEH 123

Query: 127 AITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLKAVDDLKTWLTSAATYQQTC 186
                 DN T  A+  C EL+ LA+D L+ ++++S    LK  DDL+TWL+S  TYQ+TC
Sbjct: 124 -----MDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETC 183

Query: 187 IEGLEEVD-PALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNLRRLMSYEDHQSSAD 246
           ++ L E + P+L     ++LKNSTE+TSN LAI+++  K+ D++  RR    E   +   
Sbjct: 184 MDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVV 243

Query: 247 WAH-PIL---RKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGS 306
            A  P++   R L   DL+K+A IVVAKD SGKY+TI  AL  V +K+ K T+IYVKKG 
Sbjct: 244 VADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGV 303

Query: 307 YKENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRN 366
           Y ENV VEK KWNVVM+GDG + TIV+  LNFIDGTPTF +ATFA  GKGF+A DMGF N
Sbjct: 304 YLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFIN 363

Query: 367 TAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAV 426
           TAGP+KHQAVALM ++D S+FY+C MDA+QDT+Y H+ RQFYR+C + GTVDFIFGN+AV
Sbjct: 364 TAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAV 423

Query: 427 VLQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQTYLGRPWKN 486
           V Q C ILP+ P+ GQQNTITAQGR DPNQNTGISI + +I+P  +L   QT+LGRPWK+
Sbjct: 424 VFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKD 483

Query: 487 YSTTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVR-NID 546
           +STTV M++ +   INP GWLPW G +APDTIFYAE+ N GPG+ST NRVKW+G++ ++ 
Sbjct: 484 FSTTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLT 543

Query: 547 NKTAKKFTVSSFI-GKDWISKAQVPFQS 564
            K A KFTV  FI G +W+   +VPF S
Sbjct: 544 KKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of Sgr019390 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 449.1 bits (1154), Expect = 5.1e-126
Identity = 255/583 (43.74%), Postives = 364/583 (62.44%), Query Frame = 0

Query: 7   YGKVDETDQVRLDASRRTRKRIAVIALSSVLLVGVVLAVVFGT---RNGSKNDDSDNHNA 66
           YGKVDE   + L   ++TRKR+ ++++S V+L+ V++A V  T   +N +++  S     
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  ALSASIKAVCDVTLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIFEHA 126
             S S+KA+C VT +PE+C S+   L +S+  DP  + KLS++V + EL   +D + E  
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKL 129

Query: 127 ITNSTDNKTILALKDCHELLDLALDHLSISLSASEITLLK------AVDDLKTWLTSAAT 186
              + D +   AL+ C +L++ ALD L+ ++SA +    K       ++DLKTWL++  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIEGLEEV--------DPALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLNL-- 246
             +TC + L+E+        +  +   +   +  STE TSN LAIVS        L +  
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 ---RRLMSYEDHQSSADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDK 306
              RRLMS+  HQ S D+     R+L Q+   K  D+ VA D +G   T+  A+  VP K
Sbjct: 250 HRRRRLMSHH-HQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SMKRTVIYVKKGSYKENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAK 366
           S+K  VIYVK G+Y ENV ++K+KWNV++ GDG   TI++GS NF+DGTPT+ +ATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFRNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKV 426
           GKGFI  D+G  NTAG +KHQAVA  S SD S++Y+C  D +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 YGTVDFIFGNSAVVLQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDL 486
            GT+DFIFG++AVV Q C I+P+ PL  Q NTITAQG+ DPNQ++G+SIQ  +I   G++
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNV 489

Query: 487 KSTQTYLGRPWKNYSTTVYMQNNLGSLINPNGWLPWV-GTSAPDTIFYAEFQNYGPGSST 546
               TYLGRPWK +STTV M+  +G+++ P+GW+ WV G   P +I Y E++N GPGS  
Sbjct: 490 -IAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 ANRVKWKGVRNI-DNKTAKKFTVSSFI-GKDWISKAQVPFQSS 565
             RVKW G + +  +  A KFTV++ + G DWI    V  Q S
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGVINQLS 586

BLAST of Sgr019390 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 389.8 bits (1000), Expect = 3.7e-108
Identity = 236/569 (41.48%), Postives = 338/569 (59.40%), Query Frame = 0

Query: 19  DASRRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKNDDSDNHNAALSASIKAVCDV--- 78
           D S+R R+ I VI +SSVLL+ +V+AV  G  + +K+D      A ++AS+KAV DV   
Sbjct: 7   DESKRKRRYI-VITISSVLLISMVVAVTVGV-SLNKHDGDSKGKAEVNASVKAVKDVCAP 66

Query: 79  TLYPETCYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIFEHAITNSTDNKTILA 138
           T Y +TC          +  DP ++ K +  V + +++ AA    +  +    D++T +A
Sbjct: 67  TDYRKTCEDTL-IKNGKNTTDPMELVKTAFNVTMKQITDAAKK-SQTIMELQKDSRTRMA 126

Query: 139 LKDCHELLDLALDHLSIS---LSASEITLL-KAVDDLKTWLTSAATYQQTCIEGLEEVDP 198
           L  C EL+D ALD LS S   L   E  LL +A+ +L+ WL++A ++++TC+EG +    
Sbjct: 127 LDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQG 186

Query: 199 ALKNIIADYLKNSTELTSNGLAIVSFFSKLT-----DSLNLRRLMSYEDHQSSADWAHPI 258
                +   LK + ELT NGLAI+S  S          LN RRL++    +    W    
Sbjct: 187 NAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA----EGFPSWVDQR 246

Query: 259 LRKLAQ-----SDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVKKGSYKEN 318
            RKL Q     SD++   DIVVA+D SG+YKTI  AL+ VP K     V+++K G YKE 
Sbjct: 247 GRKLLQAAAAYSDVK--PDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEY 306

Query: 319 VEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMGFRNTAGP 378
           V+V K   ++V IGDG + TI++G+ N+ DG  T+ +AT A  G  FIA ++GF NTAG 
Sbjct: 307 VQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGA 366

Query: 379 SKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGNSAVVLQN 438
            KHQAVA+   SD+SIF+ C+ D YQDTLYTHS+RQF+R+C + GT+DF+FG++A V QN
Sbjct: 367 IKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQN 426

Query: 439 CSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDL----KSTQTYLGRPWKN 498
           C++L + PL  Q   ITA GR DP ++TG   Q  +I    D     ++++ YLGRPWK 
Sbjct: 427 CTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKE 486

Query: 499 YSTTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWKGVRNIDN 558
           YS T+ M   +   + P GW PW+G     T+FY+E QN GPGS+ ANRV W G++ +  
Sbjct: 487 YSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSE 546

Query: 559 KTAKKFTVSSFI-GKDWISKAQVPFQSSL 566
           +   KFT + +I G DWI    VP+ + L
Sbjct: 547 EDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Sgr019390 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 387.1 bits (993), Expect = 2.4e-107
Identity = 235/577 (40.73%), Postives = 324/577 (56.15%), Query Frame = 0

Query: 22  RRTRKRIAVIALSSVLLVGVVLAVVFGTRNGSKNDDSDNHNAALSASIKAVCDVTLYPET 81
           +  +K I   A  ++LL+  ++ +   T N +KN      ++   A +K+VC  TLYPE 
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPEL 74

Query: 82  CYSAFGPLTNSSRLDPGQICKLSIQVALGELSRAADYIFEHAITNSTDNKT---ILALKD 141
           C+SA             ++ + S+ +    +    +Y     +       T   + AL D
Sbjct: 75  CFSAVAATGGKELTSQKEVIEASLNLTTKAVKH--NYFAVKKLIAKRKGLTPREVTALHD 134

Query: 142 CHELLDLALDHLSISLS-----ASEITLLKAVDDLKTWLTSAATYQQTCIEGL--EEVDP 201
           C E +D  LD L +++        + +L K  DDLKT ++SA T Q TC++G   ++ D 
Sbjct: 135 CLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADR 194

Query: 202 ALKNIIADYLKNSTELTSNGLAIVSFFSKLTDSLN-----------------LRRLMSYE 261
            ++  +     +   + SN LA++   ++ TD  N                 L+ +    
Sbjct: 195 KVRKALLKGQVHVEHMCSNALAMIKNMTE-TDIANFELRDKSSTFTNNNNRKLKEVTGDL 254

Query: 262 DHQSSADWAHPILRKLAQSDLRKQADIVVAKDSSGKYKTITAALKAVPDKSMKRTVIYVK 321
           D      W     R+L Q    K AD  VA D SG + T+ AA+ A P+KS KR VI++K
Sbjct: 255 DSDGWPKWLSVGDRRLLQGSTIK-ADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIK 314

Query: 322 KGSYKENVEVEKNKWNVVMIGDGMNATIVTGSLNFIDGTPTFASATFAAKGKGFIAIDMG 381
            G Y+ENVEV K K N++ +GDG   TI+TGS N +DG+ TF SAT AA G+ F+A D+ 
Sbjct: 315 AGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDIT 374

Query: 382 FRNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYTHSNRQFYRECKVYGTVDFIFGN 441
           F+NTAGPSKHQAVAL   SD S FY+C M AYQDTLY HSNRQF+ +C + GTVDFIFGN
Sbjct: 375 FQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGN 434

Query: 442 SAVVLQNCSILPKLPLLGQQNTITAQGRFDPNQNTGISIQDSSIQPFGDLKSTQ----TY 501
           +A VLQ+C I  + P  GQ+N +TAQGR DPNQNTGI IQ+  I    DL + +    TY
Sbjct: 435 AAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTY 494

Query: 502 LGRPWKNYSTTVYMQNNLGSLINPNGWLPWVGTSAPDTIFYAEFQNYGPGSSTANRVKWK 561
           LGRPWK YS TV MQ+++  +I P GW  W G+ A DT+ Y E+ N G G+ TANRVKWK
Sbjct: 495 LGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWK 554

Query: 562 GVRNIDNKT-AKKFTVSSFI-GKDWISKAQVPFQSSL 566
           G + I + T A+ FT   FI G  W++    PF  SL
Sbjct: 555 GYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140040.17.4e-28488.03putative pectinesterase/pectinesterase inhibitor 24 [Momordica charantia][more]
XP_038895610.18.1e-28387.30putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida][more]
XP_023000829.13.9e-27785.34probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima][more]
KAG6583848.12.5e-27684.98putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosp... [more]
XP_022927225.12.5e-27684.98putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SG771.5e-17055.18Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3... [more]
Q9FF788.5e-16653.52Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3... [more]
Q431117.5e-13847.17Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1[more]
Q438677.2e-12543.74Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1[more]
Q431435.6e-10942.22Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
Match NameE-valueIdentityDescription
A0A6J1CDY33.6e-28488.03Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010791 PE=3 SV=1[more]
A0A6J1KL231.9e-27785.34Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1[more]
A0A6J1ENB01.2e-27684.98Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1[more]
A0A5A7VDG14.0e-27584.66Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G0077... [more]
A0A1S3BDA44.0e-27584.66Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10710.11.1e-17155.18root hair specific 12 [more]
AT5G04960.16.0e-16753.52Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53840.15.1e-12643.74pectin methylesterase 1 [more]
AT3G05610.13.7e-10841.48Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53830.12.4e-10740.73pectin methylesterase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 64..214
e-value: 3.9E-33
score: 126.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 37..215
e-value: 3.7E-22
score: 76.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 68..214
e-value: 7.0E-26
score: 91.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 66..218
e-value: 1.4E-32
score: 114.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 68..219
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 260..551
e-value: 4.5E-139
score: 462.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 255..565
e-value: 5.5E-111
score: 373.0
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 19..561
NoneNo IPR availablePANTHERPTHR31707:SF272PECTINESTERASE/PECTINESTERASE INHIBITOR 24-RELATEDcoord: 19..561
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 71..220
e-value: 1.0034E-40
score: 142.962
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 404..413
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 285..304
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 258..559

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr019390.1Sgr019390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity