Sgr019344 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr019344
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionAlpha/beta-Hydrolases superfamily protein
Locationtig00153346: 463510 .. 469428 (-)
RNA-Seq ExpressionSgr019344
SyntenySgr019344
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAATGGAACAGCTAGGCGTCTCCTTCACAACTTCCGTAAGTGAAATTTCCTTTTAGCTCTTCCTTGTATTGGTACTGCGTCATGAATGTTTGAGAACTTGTATTTGTTTCTCGATTATGATGTTAGTGTGAGTTTAATTAATGGCTGTTTGATGATGTTCATTCACTGTATCTTTGGCTGTTTCCTTTATATAATTCTTGGAGAATCTTTACACTCTCGAACGGGATGAAATGTGATAGAATACTGTACGTTCTTTCTGTTATTTGTTTAGAACCTCGTTCGCAATCGCGACCTCTGTACTAAATTAAGAAAAAATTTGATGTTGATTTTTGTTATTTTGTTTTGTTCAATCTCTTAGTACTTTAGGATTGAGTTGGTAAATAATGTAATTTGTTCTTATTGTTTGTGAAAGGCACTATCTAGAGATTTGCTTAATCGAATTCACGATGTTATCTAACGGTGAGTTCGATTAAATTCTTTATTTTTATTTTTTTTCTAAAATAGGAAATGAAATTAGAAATTAATTTACTTGTCTTAAATGGATTCAAATGTGAATGAACTATACTATTTTGGGTCTTCCATTTGTGGTTATACTAATAAATATTGCTGCAACTATAGGCAACCTTTTTTTTGTCGTTAAATGGGCATAGCCTTTGAAATCATCTATCTGGAACTGAAATTATTAATTAGCACTGGAATGTTAGTATGCCTAACTTGGAGAGAGTGTTTTGATTATGTAAATATTTATTAAATTAAAACTTCAACTAGCTACCGAGAGGGGAAAAGTACTTTTTCCTCTTCTAATTATGATCTAGTATCGGGGATTTTAATTCTTATAATTCACATTGATGAATAATCCCAGTCACACTTCTATCTTTGATCATAAATTTTAAAGTAGCTTTCTCGGCTATCAATTTAAGGTTTCTTATTGACCACAATGTTGATGTTTTATTTTTGTGATTACCCTTTATCTCTCATTATTTTGAACTGGAGTCCTTTTTTGTAATTGTGTAGTTTGTTAGCATTTTGGTGTTTGTTCTTGGCTCCTTTTGTCTTGGGCTCTCCTTTTTCTTTTTGCTTTTTTTGTTGCCTTTGTGTATTCTTTCATTTCTTCTCAATGAAAGTTGGTTTTTCATCATAAAAATAATGTAGATGTTTTATAAATTATTAGGGGTATTTACATGGCTTGTCTTTTCTTCAACTCTGCAGATTATGCCAGAAGTTTTCTCAGGAAACAGGAGTGGCAGGCAAGCTTTGTAAATAGTGTTCTTATGCATTTTCAGAGGTCAAAAGCAGATAAAGTGGAAATAGGTGTGCATAAAGAAGGTGAAGTTTTACCTGAAGTTTCTTTAAGGTCGGAAATGGAGAAGAAAAGCAAAATAGGTTCTGAACCTAGTAAGAACTCTTCTGATGAGGAAGATATAAGTGATTGTAGTCGGAGATTAGAACTTGCCTGGCTGACAAAAGCTCTTGAACCAGCTTTGCAATTGTGTAGGTGGGCACTGTCATCAGGTTTGTTTATGTTAGTACTTCTCATCTTACTTGTGGCTTACCAAATATGGGTGGTTACCATGATAGTCCAAGTAGTCATTATTTCTTTAATTTGACCATTACTTACTTCCACGAAAAACTGTACTGATTAAATATGTTGTTGCTTAACAAACACAGTCTACTGTCTGGTAATTTTTTCTTGATGTAGTTATGTTTGTTTACGACATATGATGTCCAATACTGTTTGAGGACCCCTATTGTAAATATGTATGTTAGAAAGGGCAAGGAGGGAAATTCACCCCCCACAGTTAGTTAGGAAAGTCAGGCAGTACGGGCTGCGCGCGTATACGTAGTAGGGGGGTAAGGCTGTGCAGGGTATGTTGGTTTTTCTTTGGAATTTCAGTGTAGGGGAGAGATTACCTGCCCTCTCTAATTGGCTGGGGACCTTTTGTAAGCCCAATAAGCAGGGGAACCTTACATACTGTCTGCATTTTCTTTTGCTTTTTCATTTTTCGTTTTAAAGTTCCAGCCTGTGTCTAATTTTGTAGTCTTGGAGGCTGATTAAGTTTTTTTGGCAACTTACAGGGGGTACAGGGGGTGGAATTCGAAATCAAACTGCACCTAGAAGTCGTTCTGTTTCAGAAATCATTGCAAGTATCCAACGGAGTAAGATGGGAATCCAGGATTGGAGCTTGAGTGATCTTACAATTGGATTATACCTTATCTATCTCCGTCAGGCTTCAACAAATCCATTTGAGGACATTAAGGGTGTCCAGATCTCTTCAGATGCAATTGTAATCTGTTTGCATTTTTTTTCTTATGAAATATATGGATCCATTGAAATTATGTTTTTTTGTTTTTTATATTAAAGAATTTTCAACACTTTATAAACCCGAATTGAAGCTCTTATAGGTAATGATTTTGTGAACCAGGTCCAAGACCTCATCTACTACCTTGAACTAGCTAAAGGTTCTTATAAAGATTCTGCAGCTACTCTTGCAAGAAACAGCATGCTTCGAGAGTGCAACATATTGAAATTTATAAGGGATTCCAGTGTTATGAGGCCTGGATATTATATAGGGGTTGATACTAGAAAGAAACTTGTAATCTTTGGAATTCGAGGAACTCACACGGTATATGATCTCATCACTGATATTGTTACATCAAGTGATAGAGAAGTCACGTTCGAAGGATATTCTACACACTTTGGCACTGCTGAATCTGCTCGCTGGTTTCTTGAACATGAGATGGGAATGATAAGAAGATGCTTGGAGAAATATCAGGTGGTGAAGATTTTACTGTCTTCTACTTCTGTTGTTTCCAATGATATTTATATATACTAAAGTTCTTTTAGCTTATTCTTTACATTCCCCCATCACCCTATATCTAATTCATTAACAGGGTTTTAGGTTAAGGCTTGTGGGTCATTCTCTTGGAGGAGCTATCGCTTCGTTACTAGCTGTAATGCTACGTAAAAAGTCAAATAAGGAGCTTGGATTTAGCCCTGATATTGTTTCTGCCATTGGATATGCTACACCGCCTTGTGTCTCAATAAAACTTGCTGAGAGTTGTGCTGACTATGTTACCACTGTAGTGATGCAGGTTGCCCACATGCAGTCCTCATACTATTCCAGCAAAAGCTACTGTTTTCTAAAATAAAAATTTCCTAGATTCTTATTGTGGCAGTTCTTATTTCATTCTGTTGCTTCACTTAAATTCTCTGATTTTACTTTGGGCAGGATGATATTATCCCTCGACTAAGTATAGCTTCTCTAACAAGGCTAAGGAATGAAATTCTTCAAACTGATTGGTAAGTTATTATGCTCTATTGAAAGGCTAAAACTAATTTTCTTCCATGGATGTGCTGAGAGTAAATATGGCCATTGAAGGTGTGAGGAGACATTGGAAGCATTTTTAAAGATTATGAGATGAAGGGATTAAAAACTGTCCCTGAAAATCTCTGGAGTCTAACACTCTAAGTTAAGGGATCACGTAATCCTGTTGATAATCATTTTTATCTTTGAAAATGTTATGACCTCAATTTATATTTTGTATCTGAATATGCTCGGTTGCAATCTTGTCATAGTATCTAACGCATATTACTAAGTAGTTTATACGAAGTATCAAAGCTGATAAATCTAGTGAGGAATTGGTTTGAGAAATGCGATTTGAATTTCCTTAACGTAGGCATCTTATTCTTCAAGAATTTTGTATTAATTGGATAGTGAACTGGTAGAATTCACTCAGATTCAAAGGATATTGAAATACACATGGGGAAATTCGAGGGTCCCTTATCTCTCCCCTCCTGCTTAGTTACTTCTAGTACAAGCAAAAGCATGAATTCTTCCTTCCTCCATACTATTATTTATACCATACTACTTACCCGCCCTTTCAGATTCTAACTAAATGACTAGGGCCCACATAGACATCAATTTCCCTCCGTATCCCACTTCCTCTTATATGTTTCTATGCTTCCCCCCAAAAGAACCACCTTGTACTCAAGGTGAAAATCCAGAATCTGAGTAGCAATAGAAGAGATGTTTTCCCAAGTAGCATCCATAGACATAGCACCCTCCCATCAAATAAGGACTTCCATGACATCATCATGATCGGGAGAAGTTCGTAAATCAAGCACTTCTGCTGGATTAACTCGAACCACCATGCCACCCAAAAATATTTTCCCCATGGATTAACTCAAAGAATATCCATCTCCCACTTAGCTAAAAGAGCTTTATTTTTAAATTCCAGGCTTCCAATTCCTAAATCACCTCCTTTTGAAGGTTTAGAAAACTATGATTCCAACAGGATGTTTTAATCTCCATCCCCATTTGGCATGAAGAATAAAAATTTTAGTTATATTTATTTTGACAATTGTTTTTTGTCGTTTATCTGCAGAAAAAGGTGGTTACTCAATAGTATTACTTTTGATTAATGATTTGCTCAGCACATGACTTCTTTTACGCTGTTACGTGTTTATCCAATTACCAATTTAACAAGAACTCATAGGACTTTATTGGTTTTCATTAAATTGCATATTCAGAGCAAATAAACATTAGCTTTGGAGTACCTCAATGGTCATAACCAGCCTTGTAGTTCCTTGAACTTGAACTATAAACAATATGAATATGAGCATATCAACCTTATGTAGCTGTATAAACTAGTGATGTCAAACTTTACAATTCTATTGCGGAAGTACTATTTAGAGATATGTTCCCTGCTGATTTTTGAAACTTATTGTCAATCTGCTTTGAGGATGACCAGATTTTGAAAATGTTACATATATCATATACCATGCATCAATCTAATTGCACGAGCTATTTTGAGGGATAAAAAATAAAGTAACATATGCTGATTTCTTGTTATTGAAAAAGGTATAGGAAAAGGTAATCTCGAATTTCATGCCCTCTCTGATGCAGGATGAGTGTGATTGAAAAAGAAGACTGGAAAAGTTTCATTGATTTGGTCACAAATGCAAAGCAGGTGGTTTCATCGGTACAAGATGTTACTCGAAAACTTGCGGATTATGCAAAATTCACAAACAAGAAAAGATCCACCAGTAAGCTAGTTTTTGTTCCATCTCCGTTCCAAATAAATCAAATATTTGAAACGTGCTAGTCCTAACATTTACAGGTCTACTTTATGGCTTCCTCAGGTGTAAATTACTTGAATATTGTTGTTTTAATGGCTTGCAGTTTGTTTTGGTCATAGATTGGGGAAGATTAAGCGAGCAGTCATGTGATGAAACTTTTATTGACAACATTTTCTTTTTCTCGACTGAATTCACATCAAATTGATTATCAAACTTCTAGACTCTACACATGGCTATTCGTTTACCTTACGTTTTCAGTATCTTATTTGCCTGTTCTTTCTCGACCATAAATTTACTTGCAAATGTAAATGTTCCTTGGTCAGGATTTATCTATTTCATGTCAATTTTACAGTCTTCAATTTTTTTTCTACCACGGATGACGGAACTCTACACCGAAGTAGTTCATATTGATTTAATTAGAAGGAAATGTAGTAATCATACCCTTTTGACCTCAAAGATCCTTTTTGATCTCCTTCCCTAAACGACTTGCTTTCTTCCCTTTTAACAGATGGTAGCATTAGAAAACAGTCTCATGTGGCTTCTGGAGCTCCTTCACATCCTACTGCTGCTCAAAATGAAGCCGCAAGATGTAAAATTCCTGAGGAGCTATTTGTTCCGGGGACAGTTTATTATCTGAAGAGGAATATGGACGACCAGAGGGGCGGTGCCCCAGAATACTTCACTCTGTGGAAGCGGCATCCTGATGAACATTTCCAGCGAATAGTGCTATCAAGCAACCTTATTTCGGATCATAGATGTGATAGCCACTACTATGCTTTAAGGGATGCACTTAAAGGTCTGCCAGCTAGGAACGATGAAGGTATCTTCACTTAA

mRNA sequence

ATGGCTAATGGAACAGCTAGGCGTCTCCTTCACAACTTCCATTATGCCAGAAGTTTTCTCAGGAAACAGGAGTGGCAGGCAAGCTTTGTAAATAGTGTTCTTATGCATTTTCAGAGGTCAAAAGCAGATAAAGTGGAAATAGGTGTGCATAAAGAAGGTGAAGTTTTACCTGAAGTTTCTTTAAGGTCGGAAATGGAGAAGAAAAGCAAAATAGGTTCTGAACCTAGTAAGAACTCTTCTGATGAGGAAGATATAAGTGATTGTAGTCGGAGATTAGAACTTGCCTGGCTGACAAAAGCTCTTGAACCAGCTTTGCAATTGTGTAGGTGGGCACTGTCATCAGGGGGTACAGGGGGTGGAATTCGAAATCAAACTGCACCTAGAAGTCGTTCTGTTTCAGAAATCATTGCAAGTATCCAACGGAGTAAGATGGGAATCCAGGATTGGAGCTTGAGTGATCTTACAATTGGATTATACCTTATCTATCTCCGTCAGGCTTCAACAAATCCATTTGAGGACATTAAGGGTGTCCAGATCTCTTCAGATGCAATTGTCCAAGACCTCATCTACTACCTTGAACTAGCTAAAGGTTCTTATAAAGATTCTGCAGCTACTCTTGCAAGAAACAGCATGCTTCGAGAGTGCAACATATTGAAATTTATAAGGGATTCCAGTGTTATGAGGCCTGGATATTATATAGGGGTTGATACTAGAAAGAAACTTGTAATCTTTGGAATTCGAGGAACTCACACGGTATATGATCTCATCACTGATATTGTTACATCAAGTGATAGAGAAGTCACGTTCGAAGGATATTCTACACACTTTGGCACTGCTGAATCTGCTCGCTGGTTTCTTGAACATGAGATGGGAATGATAAGAAGATGCTTGGAGAAATATCAGGGTTTTAGGTTAAGGCTTGTGGGTCATTCTCTTGGAGGAGCTATCGCTTCGTTACTAGCTGTAATGCTACGTAAAAAGTCAAATAAGGAGCTTGGATTTAGCCCTGATATTGTTTCTGCCATTGGATATGCTACACCGCCTTGTGTCTCAATAAAACTTGCTGAGAGTTGTGCTGACTATGTTACCACTGTAGTGATGCAGGATGATATTATCCCTCGACTAAGTATAGCTTCTCTAACAAGGCTAAGGAATGAAATTCTTCAAACTGATTGGATGAGTGTGATTGAAAAAGAAGACTGGAAAAGTTTCATTGATTTGGTCACAAATGCAAAGCAGGTGGTTTCATCGGTACAAGATGTTACTCGAAAACTTGCGGATTATGCAAAATTCACAAACAAGAAAAGATCCACCAATGGTAGCATTAGAAAACAGTCTCATGTGGCTTCTGGAGCTCCTTCACATCCTACTGCTGCTCAAAATGAAGCCGCAAGATGTAAAATTCCTGAGGAGCTATTTGTTCCGGGGACAGTTTATTATCTGAAGAGGAATATGGACGACCAGAGGGGCGGTGCCCCAGAATACTTCACTCTGTGGAAGCGGCATCCTGATGAACATTTCCAGCGAATAGTGCTATCAAGCAACCTTATTTCGGATCATAGATGTGATAGCCACTACTATGCTTTAAGGGATGCACTTAAAGGTCTGCCAGCTAGGAACGATGAAGGTATCTTCACTTAA

Coding sequence (CDS)

ATGGCTAATGGAACAGCTAGGCGTCTCCTTCACAACTTCCATTATGCCAGAAGTTTTCTCAGGAAACAGGAGTGGCAGGCAAGCTTTGTAAATAGTGTTCTTATGCATTTTCAGAGGTCAAAAGCAGATAAAGTGGAAATAGGTGTGCATAAAGAAGGTGAAGTTTTACCTGAAGTTTCTTTAAGGTCGGAAATGGAGAAGAAAAGCAAAATAGGTTCTGAACCTAGTAAGAACTCTTCTGATGAGGAAGATATAAGTGATTGTAGTCGGAGATTAGAACTTGCCTGGCTGACAAAAGCTCTTGAACCAGCTTTGCAATTGTGTAGGTGGGCACTGTCATCAGGGGGTACAGGGGGTGGAATTCGAAATCAAACTGCACCTAGAAGTCGTTCTGTTTCAGAAATCATTGCAAGTATCCAACGGAGTAAGATGGGAATCCAGGATTGGAGCTTGAGTGATCTTACAATTGGATTATACCTTATCTATCTCCGTCAGGCTTCAACAAATCCATTTGAGGACATTAAGGGTGTCCAGATCTCTTCAGATGCAATTGTCCAAGACCTCATCTACTACCTTGAACTAGCTAAAGGTTCTTATAAAGATTCTGCAGCTACTCTTGCAAGAAACAGCATGCTTCGAGAGTGCAACATATTGAAATTTATAAGGGATTCCAGTGTTATGAGGCCTGGATATTATATAGGGGTTGATACTAGAAAGAAACTTGTAATCTTTGGAATTCGAGGAACTCACACGGTATATGATCTCATCACTGATATTGTTACATCAAGTGATAGAGAAGTCACGTTCGAAGGATATTCTACACACTTTGGCACTGCTGAATCTGCTCGCTGGTTTCTTGAACATGAGATGGGAATGATAAGAAGATGCTTGGAGAAATATCAGGGTTTTAGGTTAAGGCTTGTGGGTCATTCTCTTGGAGGAGCTATCGCTTCGTTACTAGCTGTAATGCTACGTAAAAAGTCAAATAAGGAGCTTGGATTTAGCCCTGATATTGTTTCTGCCATTGGATATGCTACACCGCCTTGTGTCTCAATAAAACTTGCTGAGAGTTGTGCTGACTATGTTACCACTGTAGTGATGCAGGATGATATTATCCCTCGACTAAGTATAGCTTCTCTAACAAGGCTAAGGAATGAAATTCTTCAAACTGATTGGATGAGTGTGATTGAAAAAGAAGACTGGAAAAGTTTCATTGATTTGGTCACAAATGCAAAGCAGGTGGTTTCATCGGTACAAGATGTTACTCGAAAACTTGCGGATTATGCAAAATTCACAAACAAGAAAAGATCCACCAATGGTAGCATTAGAAAACAGTCTCATGTGGCTTCTGGAGCTCCTTCACATCCTACTGCTGCTCAAAATGAAGCCGCAAGATGTAAAATTCCTGAGGAGCTATTTGTTCCGGGGACAGTTTATTATCTGAAGAGGAATATGGACGACCAGAGGGGCGGTGCCCCAGAATACTTCACTCTGTGGAAGCGGCATCCTGATGAACATTTCCAGCGAATAGTGCTATCAAGCAACCTTATTTCGGATCATAGATGTGATAGCCACTACTATGCTTTAAGGGATGCACTTAAAGGTCTGCCAGCTAGGAACGATGAAGGTATCTTCACTTAA

Protein sequence

MANGTARRLLHNFHYARSFLRKQEWQASFVNSVLMHFQRSKADKVEIGVHKEGEVLPEVSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTGGGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTRKKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLEKYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESCADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSVQDVTRKLADYAKFTNKKRSTNGSIRKQSHVASGAPSHPTAAQNEAARCKIPEELFVPGTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALRDALKGLPARNDEGIFT
Homology
BLAST of Sgr019344 vs. NCBI nr
Match: XP_022156622.1 (uncharacterized protein LOC111023479 [Momordica charantia])

HSP 1 Score: 911.8 bits (2355), Expect = 2.9e-261
Identity = 481/551 (87.30%), Postives = 505/551 (91.65%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQEWQAS--FVNSVLMHFQRSKADKVEIGVHKEGEVLPE 60
           MA+G  RRLLHNFH+ARSFLRKQE QAS   VNSVLMHF RSKADKVEIGVHKEGEVLPE
Sbjct: 1   MASGAVRRLLHNFHHARSFLRKQELQASSVTVNSVLMHFHRSKADKVEIGVHKEGEVLPE 60

Query: 61  VSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTG 120
           VS+RSEMEK SK GSEP+K  SD+E+ISD  RR ELAWLTKALEPALQL RWALS+  +G
Sbjct: 61  VSVRSEMEKNSKKGSEPTKYFSDDENISDSKRRSELAWLTKALEPALQLYRWALSA--SG 120

Query: 121 GGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQ 180
            GIR+QT PRSRSVSEIIASIQ SKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDI GVQ
Sbjct: 121 DGIRDQTPPRSRSVSEIIASIQWSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDISGVQ 180

Query: 181 ISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTR 240
           ISSDAIVQDLIYYLELAKGSYKDSAA+LARNSMLRECNILKF+ DSSVMRPGYYIGVDTR
Sbjct: 181 ISSDAIVQDLIYYLELAKGSYKDSAASLARNSMLRECNILKFVGDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLE 300
           KKLVIFGIRGTHT+ DLITDIVTSSD EVTFEGY THFGTAESARWFL+HEMGMIRRCLE
Sbjct: 241 KKLVIFGIRGTHTLSDLITDIVTSSDGEVTFEGYVTHFGTAESARWFLDHEMGMIRRCLE 300

Query: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESC 360
           KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVS KLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSV 420
           ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDW SVIEK+DWKSFI LVTNA+QVVSSV
Sbjct: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWTSVIEKQDWKSFIGLVTNARQVVSSV 420

Query: 421 QDVTRKLADYAKFTNKKRSTNGSIRKQSHVASGAPSHPT-AAQNEA--ARCKIPEELFVP 480
           QDV RKLADYAKFT+KK S++GSIRK+SHVAS AP HPT AA+N+A  AR K PEELFVP
Sbjct: 421 QDVARKLADYAKFTSKKISSDGSIRKESHVAS-APPHPTAAARNKAATARYKTPEELFVP 480

Query: 481 GTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALRDALKG 540
           GTVYYLKRN +    G PEYFTLWKRHP EHFQRIVLSSNLISDHRCDSHYYALRD LKG
Sbjct: 481 GTVYYLKRNTN----GTPEYFTLWKRHPGEHFQRIVLSSNLISDHRCDSHYYALRDVLKG 540

Query: 541 LPARNDEGIFT 547
           LPAR+DE IF+
Sbjct: 541 LPARSDESIFS 544

BLAST of Sgr019344 vs. NCBI nr
Match: XP_023513693.1 (sn1-specific diacylglycerol lipase beta [Cucurbita pepo subsp. pepo])

HSP 1 Score: 846.3 bits (2185), Expect = 1.5e-241
Identity = 447/556 (80.40%), Postives = 488/556 (87.77%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQEWQAS--FVNSVLMHFQRSKADKVEIGVHKEGEVLPE 60
           M+NGTARRLLHN + ARS LRKQ+WQAS   VNSVLMH QRSKAD VE  V KE EVLPE
Sbjct: 1   MSNGTARRLLHNLYSARSSLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTG 120
           VS+RSEMEK SK GS+  K  SD EDISD +RRLELAWLTKAL+ A QL RWALSS   G
Sbjct: 61  VSVRSEMEKNSKEGSKTPKYFSDAEDISDSNRRLELAWLTKALKSASQLYRWALSS---G 120

Query: 121 GGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQ 180
            GI+NQ  PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL+LIYLRQASTNP  DIKGVQ
Sbjct: 121 DGIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQ 180

Query: 181 ISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTR 240
           I+S+AIV+DLIYYLELAKG YKDSAA LARN+MLRE NILKFI+DSSVMRPGYYIGVDTR
Sbjct: 181 ITSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLE 300
           KKLVIFGIRGTH+VYDLITDI TSSD EVT EGYSTHFGT+ESARWFL+HE+ MIRRCL+
Sbjct: 241 KKLVIFGIRGTHSVYDLITDITTSSDEEVTLEGYSTHFGTSESARWFLDHEIEMIRRCLK 300

Query: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESC 360
           KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIG+ATPPCVS KLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSV 420
           ADYVTTVVMQDDIIPRLS+ASLT+LR EILQTDW SVIE++DWK+   L+TNAKQVV+SV
Sbjct: 361 ADYVTTVVMQDDIIPRLSLASLTKLRVEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTSV 420

Query: 421 QDVTRKLADYAKFTNKKRSTNGSIRKQSHVASG-APSHPTA-------AQNEAARCKIPE 480
           QDV RKL DYAKFT+KK  ++ S  K SHVASG  PSHPTA       AQNEAARCKIPE
Sbjct: 421 QDVARKLGDYAKFTSKKGPSDDSYGKGSHVASGPPPSHPTAVVQRADTAQNEAARCKIPE 480

Query: 481 ELFVPGTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALR 540
           ELFVPGTVYYLK++++ +   A EYFTLWKRHPDEHFQ+IVLSSNLISDH+CDSHY+ALR
Sbjct: 481 ELFVPGTVYYLKKHVNLEE--AYEYFTLWKRHPDEHFQQIVLSSNLISDHKCDSHYHALR 540

Query: 541 DALKGLPARNDEGIFT 547
           D LKGLP+ NDEGIF+
Sbjct: 541 DVLKGLPSCNDEGIFS 551

BLAST of Sgr019344 vs. NCBI nr
Match: XP_022959891.1 (sn1-specific diacylglycerol lipase beta [Cucurbita moschata])

HSP 1 Score: 843.2 bits (2177), Expect = 1.3e-240
Identity = 446/556 (80.22%), Postives = 487/556 (87.59%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQEWQAS--FVNSVLMHFQRSKADKVEIGVHKEGEVLPE 60
           M+NGTARRLLHN + ARS LRKQ+WQAS   VNSVLMH QRSKAD VE  V KE EVLPE
Sbjct: 1   MSNGTARRLLHNLYSARSSLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTG 120
           VS+RSEMEK SK GS+  K  SD EDISD +RRLELAWLTKAL+ A QL RWALSS   G
Sbjct: 61  VSVRSEMEKNSKEGSKTPKYFSDAEDISDSNRRLELAWLTKALKSASQLYRWALSS---G 120

Query: 121 GGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQ 180
            GI+NQ  PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL+LIYLRQASTNP  DIKGVQ
Sbjct: 121 DGIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQ 180

Query: 181 ISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTR 240
           I+S+AIV+DLIYYLELAKG YKDSAA LARN+MLRE NILKFI+DSSVMRPGYYIGVDTR
Sbjct: 181 ITSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLE 300
           KKLVIFGIRGTH+VYDLITDI TSSD EVT EGYSTHFGT+ESARWFL+HE+ MIRRCL+
Sbjct: 241 KKLVIFGIRGTHSVYDLITDITTSSDEEVTLEGYSTHFGTSESARWFLDHEIEMIRRCLK 300

Query: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESC 360
           KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIG+ATPPCVS KLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSV 420
           ADYVTTVVMQDDIIPRLS+ASLT+LR EILQTDW SVIE++DWK+   L+TNAKQVV+SV
Sbjct: 361 ADYVTTVVMQDDIIPRLSLASLTKLRVEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTSV 420

Query: 421 QDVTRKLADYAKFTNKKRSTNGSIRKQSHVASG-APSHPTA-------AQNEAARCKIPE 480
           QDV RKL DYAKFT+KK  ++ S  K SHVASG  PS PTA       AQNEAARCKIPE
Sbjct: 421 QDVARKLGDYAKFTSKKGPSDDSYGKGSHVASGPPPSQPTALVQRADTAQNEAARCKIPE 480

Query: 481 ELFVPGTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALR 540
           ELFVPGTVYYLK++++ +   A EYFTLWKRHPDEHFQ+IVLSSNLISDH+CDSHY+ALR
Sbjct: 481 ELFVPGTVYYLKKHVNLEE--AYEYFTLWKRHPDEHFQQIVLSSNLISDHKCDSHYHALR 540

Query: 541 DALKGLPARNDEGIFT 547
           D LKGLP+ NDEGIF+
Sbjct: 541 DVLKGLPSCNDEGIFS 551

BLAST of Sgr019344 vs. NCBI nr
Match: XP_023004816.1 (sn1-specific diacylglycerol lipase beta [Cucurbita maxima])

HSP 1 Score: 838.6 bits (2165), Expect = 3.1e-239
Identity = 443/556 (79.68%), Postives = 486/556 (87.41%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQEWQAS--FVNSVLMHFQRSKADKVEIGVHKEGEVLPE 60
           M+NGTARRLL N + ARS+LRKQ+WQAS   VNSVLMH QRSKAD VE  V KE EVLPE
Sbjct: 1   MSNGTARRLLRNLYSARSYLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTG 120
           VS+RSEMEK  K GS+  K  SD EDISD +RRLELAWLTKAL+ A QL RWALSS   G
Sbjct: 61  VSVRSEMEKNIKKGSKTPKYFSDAEDISDSNRRLELAWLTKALKSASQLYRWALSS---G 120

Query: 121 GGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQ 180
            GI+NQ  PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL+LIYLRQASTNP  DIKGVQ
Sbjct: 121 DGIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQ 180

Query: 181 ISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTR 240
           I+S+AIV+DLIYYLELAKG YKDSAA LARN+MLRE NILKFI+DSSVMRPGYYIGVDTR
Sbjct: 181 ITSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLE 300
           KKLVIFGIRGTH+VYDLITDI TSSD EVTFEGYSTHFGT+ESARWFL+HE+ MIR+CL+
Sbjct: 241 KKLVIFGIRGTHSVYDLITDITTSSDEEVTFEGYSTHFGTSESARWFLDHEIEMIRQCLK 300

Query: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESC 360
           KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGF PDIVSAIG+ATPPCVS KLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFRPDIVSAIGFATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSV 420
           ADYVTTVVMQDDIIPRLS+ASLT+LR EILQTDW SVIE++DWK+   L+TNAKQVV+SV
Sbjct: 361 ADYVTTVVMQDDIIPRLSLASLTKLRIEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTSV 420

Query: 421 QDVTRKLADYAKFTNKKRSTNGSIRKQSHVASG-APSHPTA-------AQNEAARCKIPE 480
           QDV RKL DYAKFT+KK  ++ S  K SHVASG  PS PTA       AQNEAARCKIPE
Sbjct: 421 QDVARKLGDYAKFTSKKGPSDDSYGKGSHVASGPPPSQPTAVVQRADTAQNEAARCKIPE 480

Query: 481 ELFVPGTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALR 540
           ELFVPGTVYYLK++++ +   A EYFTLWKRHPDEHFQ+IVLSSNLISDH+CDSHY+ALR
Sbjct: 481 ELFVPGTVYYLKKHVNLEE--AYEYFTLWKRHPDEHFQQIVLSSNLISDHKCDSHYHALR 540

Query: 541 DALKGLPARNDEGIFT 547
           D LKGLP+ NDEGIF+
Sbjct: 541 DVLKGLPSCNDEGIFS 551

BLAST of Sgr019344 vs. NCBI nr
Match: XP_038897252.1 (uncharacterized protein LOC120085373 isoform X1 [Benincasa hispida])

HSP 1 Score: 837.8 bits (2163), Expect = 5.3e-239
Identity = 434/547 (79.34%), Postives = 482/547 (88.12%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQEWQAS--FVNSVLMHFQRSKADKVEIGVHKEGEVLPE 60
           MANGTARRLLHNFHYAR+FLRKQ+WQA    VN+VLMHFQR+K DKVE GVHKEGEVL E
Sbjct: 1   MANGTARRLLHNFHYARNFLRKQQWQARSVTVNNVLMHFQRTKVDKVEKGVHKEGEVLQE 60

Query: 61  VSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTG 120
           VS RSEM K SK GS+ +K+ S+ ED +D +R LELAW TKA+EPALQL RWALSSG   
Sbjct: 61  VSGRSEMGKNSKKGSKRTKSVSEAEDTTDSNRMLELAWWTKAIEPALQLYRWALSSG--- 120

Query: 121 GGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQ 180
               +   PR+RSVSEIIASIQRSK GIQDWSLSDLTIGL LIYLRQASTNP ED+KGVQ
Sbjct: 121 ----DGIPPRTRSVSEIIASIQRSKTGIQDWSLSDLTIGLCLIYLRQASTNPLEDLKGVQ 180

Query: 181 ISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTR 240
           ISSDA+V+DLIY++ELAKGSYK+SAA LA+ +MLRECNILKF+++SSVMRPGYYIGVDTR
Sbjct: 181 ISSDAMVEDLIYFVELAKGSYKNSAAMLAKTTMLRECNILKFVKNSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLE 300
           KKLVIFGIRGT+TVYDLITDI+T+SD +VTFEGYS HFGT+ESARWFL+HE+GMIRRCLE
Sbjct: 241 KKLVIFGIRGTYTVYDLITDIITTSDGDVTFEGYSMHFGTSESARWFLQHEIGMIRRCLE 300

Query: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESC 360
           KYQGFRL+LVGHSLGGAIASLLAVMLRK SNKELGFSPDIVSAIGYATPPCVS KLAESC
Sbjct: 301 KYQGFRLKLVGHSLGGAIASLLAVMLRKMSNKELGFSPDIVSAIGYATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSV 420
           ADYVTTVVMQ D+IPRLS+ASLTRLR EILQTDWMS+I+KEDWKS I LVTNAKQVV+SV
Sbjct: 361 ADYVTTVVMQGDVIPRLSVASLTRLRIEILQTDWMSLIDKEDWKSVIGLVTNAKQVVTSV 420

Query: 421 QDVTRKLADYAKFTNKKRSTNGSIRKQSHVASGAPSHPTAA-------QNEAARCKIPEE 480
           QDV RKLAD+AKF +KKRS++ S RK+S VASG+P HPTAA       QNEAARCKIP+E
Sbjct: 421 QDVARKLADFAKFPSKKRSSDDSNRKKSDVASGSPPHPTAALQSATATQNEAARCKIPDE 480

Query: 481 LFVPGTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALRD 539
           LFVPGTVYYLKR+ D      PEYFTLWKR+PDEHFQ+IVLSS LISDH CDSHYYALRD
Sbjct: 481 LFVPGTVYYLKRHTD----STPEYFTLWKRYPDEHFQQIVLSSILISDHTCDSHYYALRD 536

BLAST of Sgr019344 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 5.5e-13
Identity = 80/310 (25.81%), Postives = 146/310 (47.10%), Query Frame = 0

Query: 231 YYIGVDTRKKLVIFGIRGTHTVYDLITDIVTSSDR---EVTFEGYSTHFGTAESARWFLE 290
           + + +D RK+ V+  +RGT ++ D++TD+   S+    ++  +    H G A++AR+   
Sbjct: 358 FIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESENLELDIELQDCVAHKGIAQAARYIYR 417

Query: 291 HEM--GMIRRCLEKYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYA 350
             +  G++ +       +RL +VGHSLG   A+LLA+MLR       G  P  V A  ++
Sbjct: 418 RLVNDGILSQAFSVAPEYRLVVVGHSLGAGAAALLAIMLR-------GAYPQ-VRAYAFS 477

Query: 351 TP-PCVSIKLAESCADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSF 410
            P   +S  L E   D+V ++++  D+IPRLS+A++  L+  IL+     VI   +   +
Sbjct: 478 PPRGLLSKSLFEYSKDFVVSLILGMDVIPRLSVANMEDLKRRILR-----VIANCNKPKY 537

Query: 411 IDLVTNAKQVV--SSVQDVTRKLADYAKFTNKKRSTNGSIRKQSHVASGAPSHPTAAQNE 470
             L+      V   S  +   +L +     N+   T   + +Q+ +   +P +  +  + 
Sbjct: 538 KILLHGCWYSVFGGSPDNFPTELDE----GNQGALTQPLLGEQTLLTRCSPGY-CSGDSP 597

Query: 471 AARCKIPEELFVPGTVYYLKRNMDDQRGG---APEYFTLWKRHPDEHFQRIVLSSNLISD 530
               K P  L+ PG + +L+      R G   A +Y   W    +  F +I++   ++ D
Sbjct: 598 LDSPKYP-TLYPPGRIIHLEEEGGSGRFGCCSAAQYRARWAH--ETEFSKILIGPKMLID 646

BLAST of Sgr019344 vs. ExPASy Swiss-Prot
Match: Q8NCG7 (Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 2.7e-12
Identity = 71/302 (23.51%), Postives = 137/302 (45.36%), Query Frame = 0

Query: 231 YYIGVDTRKKLVIFGIRGTHTVYDLITDIVTSS---DREVTFEGYSTHFGTAESARWFLE 290
           + + +D RK+ V+  +RGT ++ D++TD+   S   D E   +    H G +++AR+  +
Sbjct: 358 FLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDVECEVQDRLAHKGISQAARYVYQ 417

Query: 291 HEM--GMIRRCLEKYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYA 350
             +  G++ +       +RL +VGHSLGG  A+LLA MLR    +        V    ++
Sbjct: 418 RLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATMLRAAYPQ--------VRCYAFS 477

Query: 351 TPPCV-SIKLAESCADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSF 410
            P  + S  L E    ++ ++V+  D+IPRLS+ +L  L+  IL+   ++   K  +K  
Sbjct: 478 PPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRILRV--VAHCNKPKYKIL 537

Query: 411 IDLVTNAKQVVSSVQDVTRKLADYAKFTNKKRSTNGSIRKQSHVASGAPSHPTAAQNEAA 470
           +  +                L       +++  T   + +QS +   +P++  ++ +   
Sbjct: 538 LHGLW-----YELFGGNPNNLPTELDGGDQEVLTQPLLGEQSLLTRWSPAYSFSSDSPLD 597

Query: 471 RCKIPEELFVPGTVYYLKRNMDDQRGG---APEYFTLWKRHPDEHFQRIVLSSNLISDHR 524
                  L+ PG + +L+      R G   A  Y   W    +  F +I++   +++DH 
Sbjct: 598 SSPKYPPLYPPGRIIHLQEEGASGRFGCCSAAHYSAKWSH--EAEFSKILIGPKMLTDHM 642

BLAST of Sgr019344 vs. ExPASy Swiss-Prot
Match: Q91WC9 (Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 4.7e-12
Identity = 86/370 (23.24%), Postives = 164/370 (44.32%), Query Frame = 0

Query: 169 NPFEDIKGVQISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMR 228
           NPF  +   +I  D      I Y  +    +    A++ + + L+  + +       V  
Sbjct: 298 NPFTGL--CRIGGDCCRARDIEYDAVEGDQHNCHFASILKTTGLQYRDFIHISFHDKVYE 357

Query: 229 PGYYIGVDTRKKLVIFGIRGTHTVYDLITDIVTSSDR---EVTFEGYSTHFGTAESARWF 288
             + + +D RK+ V+  +RGT ++ D++TD+   S+     +  +    H G A++AR+ 
Sbjct: 358 LPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESETLELGIELQDCVAHKGIAQAARYI 417

Query: 289 LEHEM--GMIRRCLEKYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIG 348
               +  G++ +       ++L LVGHSLG   A+LLA+MLR       G  P  V A  
Sbjct: 418 HRRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIMLR-------GAYPQ-VRAYA 477

Query: 349 YATP-PCVSIKLAESCADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWK 408
           ++ P   +S  L E   D+V ++++  D+IPRLS+ ++  L+  IL+   ++   K  +K
Sbjct: 478 FSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLSVTNMEDLKRRILRV--IANCNKPKYK 537

Query: 409 SFIDLVTNAKQVVSSVQDVTRKLADYAKFTNKKRSTNGSIRKQSHVASGAPSHPTAAQNE 468
             +           S  +   +L +      +   T   + +Q+ +   +P + ++    
Sbjct: 538 ILLHGCWYG-LFGGSPDNFPTELDE----GTQGALTQPLLGEQTLLTRYSPGYCSSDSPL 597

Query: 469 AARCKIPEELFVPGTVYYLKRNMDDQRGG---APEYFTLWKRHPDEHFQRIVLSSNLISD 528
            +  K P  L+ PG + +L+      R G   A +Y   W    +  F +I++   ++ D
Sbjct: 598 DSPTKYP-TLYPPGRIIHLEEEGGSGRFGCCSAAQYRARWAH--EAEFSKILIGPKMLID 647

Query: 529 HRCDSHYYAL 530
           H  D    AL
Sbjct: 658 HMPDVMIRAL 647

BLAST of Sgr019344 vs. ExPASy Swiss-Prot
Match: Q9Y4D2 (Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3)

HSP 1 Score: 62.4 bits (150), Expect = 1.8e-08
Identity = 52/193 (26.94%), Postives = 92/193 (47.67%), Query Frame = 0

Query: 225 SVMRPGYYIGVDTRKKLVIFGIRGTHTVYDLITDIVTSSDR------EVTFEGYSTHFGT 284
           +V    +Y+ VD  KK V+  IRGT +  D +TD+   ++R        T+ G+     +
Sbjct: 376 AVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLS 435

Query: 285 AESARWFLEHEMGMIRRCLEKYQG-----FRLRLVGHSLGGAIASLLAVMLRKKSNKELG 344
           AE  +  LE EM ++ +   +  G     + L +VGHSLG   A++L+ +LR +      
Sbjct: 436 AEYIKKKLEQEM-VLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQY----- 495

Query: 345 FSPDIVSAIGYATP-PCVSIKLAESCADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDW 404
                +    Y+ P   +S    E   ++VT VV+  D++PR+ ++ L   R ++L  D 
Sbjct: 496 ---PTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLL--DV 555

Query: 405 MSVIEKEDWKSFI 406
           +    K  W+  +
Sbjct: 556 LQRSTKPKWRIIV 557

BLAST of Sgr019344 vs. ExPASy Swiss-Prot
Match: Q6WQJ1 (Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 1.8e-08
Identity = 52/193 (26.94%), Postives = 92/193 (47.67%), Query Frame = 0

Query: 225 SVMRPGYYIGVDTRKKLVIFGIRGTHTVYDLITDIVTSSDR------EVTFEGYSTHFGT 284
           +V    +Y+ VD  KK V+  IRGT +  D +TD+   ++R        T+ G+     +
Sbjct: 376 AVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLS 435

Query: 285 AESARWFLEHEMGMIRRCLEKYQG-----FRLRLVGHSLGGAIASLLAVMLRKKSNKELG 344
           AE  +  LE EM ++ +   +  G     + L +VGHSLG   A++L+ +LR +      
Sbjct: 436 AEYIKKKLEQEM-VLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQY----- 495

Query: 345 FSPDIVSAIGYATP-PCVSIKLAESCADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDW 404
                +    Y+ P   +S    E   ++VT VV+  D++PR+ ++ L   R ++L  D 
Sbjct: 496 ---PTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLL--DV 555

Query: 405 MSVIEKEDWKSFI 406
           +    K  W+  +
Sbjct: 556 LQRSTKPKWRIIV 557

BLAST of Sgr019344 vs. ExPASy TrEMBL
Match: A0A6J1DSG2 (uncharacterized protein LOC111023479 OS=Momordica charantia OX=3673 GN=LOC111023479 PE=4 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.4e-261
Identity = 481/551 (87.30%), Postives = 505/551 (91.65%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQEWQAS--FVNSVLMHFQRSKADKVEIGVHKEGEVLPE 60
           MA+G  RRLLHNFH+ARSFLRKQE QAS   VNSVLMHF RSKADKVEIGVHKEGEVLPE
Sbjct: 1   MASGAVRRLLHNFHHARSFLRKQELQASSVTVNSVLMHFHRSKADKVEIGVHKEGEVLPE 60

Query: 61  VSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTG 120
           VS+RSEMEK SK GSEP+K  SD+E+ISD  RR ELAWLTKALEPALQL RWALS+  +G
Sbjct: 61  VSVRSEMEKNSKKGSEPTKYFSDDENISDSKRRSELAWLTKALEPALQLYRWALSA--SG 120

Query: 121 GGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQ 180
            GIR+QT PRSRSVSEIIASIQ SKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDI GVQ
Sbjct: 121 DGIRDQTPPRSRSVSEIIASIQWSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDISGVQ 180

Query: 181 ISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTR 240
           ISSDAIVQDLIYYLELAKGSYKDSAA+LARNSMLRECNILKF+ DSSVMRPGYYIGVDTR
Sbjct: 181 ISSDAIVQDLIYYLELAKGSYKDSAASLARNSMLRECNILKFVGDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLE 300
           KKLVIFGIRGTHT+ DLITDIVTSSD EVTFEGY THFGTAESARWFL+HEMGMIRRCLE
Sbjct: 241 KKLVIFGIRGTHTLSDLITDIVTSSDGEVTFEGYVTHFGTAESARWFLDHEMGMIRRCLE 300

Query: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESC 360
           KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVS KLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSV 420
           ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDW SVIEK+DWKSFI LVTNA+QVVSSV
Sbjct: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWTSVIEKQDWKSFIGLVTNARQVVSSV 420

Query: 421 QDVTRKLADYAKFTNKKRSTNGSIRKQSHVASGAPSHPT-AAQNEA--ARCKIPEELFVP 480
           QDV RKLADYAKFT+KK S++GSIRK+SHVAS AP HPT AA+N+A  AR K PEELFVP
Sbjct: 421 QDVARKLADYAKFTSKKISSDGSIRKESHVAS-APPHPTAAARNKAATARYKTPEELFVP 480

Query: 481 GTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALRDALKG 540
           GTVYYLKRN +    G PEYFTLWKRHP EHFQRIVLSSNLISDHRCDSHYYALRD LKG
Sbjct: 481 GTVYYLKRNTN----GTPEYFTLWKRHPGEHFQRIVLSSNLISDHRCDSHYYALRDVLKG 540

Query: 541 LPARNDEGIFT 547
           LPAR+DE IF+
Sbjct: 541 LPARSDESIFS 544

BLAST of Sgr019344 vs. ExPASy TrEMBL
Match: A0A6J1H649 (sn1-specific diacylglycerol lipase beta OS=Cucurbita moschata OX=3662 GN=LOC111460812 PE=4 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 6.2e-241
Identity = 446/556 (80.22%), Postives = 487/556 (87.59%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQEWQAS--FVNSVLMHFQRSKADKVEIGVHKEGEVLPE 60
           M+NGTARRLLHN + ARS LRKQ+WQAS   VNSVLMH QRSKAD VE  V KE EVLPE
Sbjct: 1   MSNGTARRLLHNLYSARSSLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTG 120
           VS+RSEMEK SK GS+  K  SD EDISD +RRLELAWLTKAL+ A QL RWALSS   G
Sbjct: 61  VSVRSEMEKNSKEGSKTPKYFSDAEDISDSNRRLELAWLTKALKSASQLYRWALSS---G 120

Query: 121 GGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQ 180
            GI+NQ  PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL+LIYLRQASTNP  DIKGVQ
Sbjct: 121 DGIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQ 180

Query: 181 ISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTR 240
           I+S+AIV+DLIYYLELAKG YKDSAA LARN+MLRE NILKFI+DSSVMRPGYYIGVDTR
Sbjct: 181 ITSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLE 300
           KKLVIFGIRGTH+VYDLITDI TSSD EVT EGYSTHFGT+ESARWFL+HE+ MIRRCL+
Sbjct: 241 KKLVIFGIRGTHSVYDLITDITTSSDEEVTLEGYSTHFGTSESARWFLDHEIEMIRRCLK 300

Query: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESC 360
           KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIG+ATPPCVS KLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSV 420
           ADYVTTVVMQDDIIPRLS+ASLT+LR EILQTDW SVIE++DWK+   L+TNAKQVV+SV
Sbjct: 361 ADYVTTVVMQDDIIPRLSLASLTKLRVEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTSV 420

Query: 421 QDVTRKLADYAKFTNKKRSTNGSIRKQSHVASG-APSHPTA-------AQNEAARCKIPE 480
           QDV RKL DYAKFT+KK  ++ S  K SHVASG  PS PTA       AQNEAARCKIPE
Sbjct: 421 QDVARKLGDYAKFTSKKGPSDDSYGKGSHVASGPPPSQPTALVQRADTAQNEAARCKIPE 480

Query: 481 ELFVPGTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALR 540
           ELFVPGTVYYLK++++ +   A EYFTLWKRHPDEHFQ+IVLSSNLISDH+CDSHY+ALR
Sbjct: 481 ELFVPGTVYYLKKHVNLEE--AYEYFTLWKRHPDEHFQQIVLSSNLISDHKCDSHYHALR 540

Query: 541 DALKGLPARNDEGIFT 547
           D LKGLP+ NDEGIF+
Sbjct: 541 DVLKGLPSCNDEGIFS 551

BLAST of Sgr019344 vs. ExPASy TrEMBL
Match: A0A6J1KT65 (sn1-specific diacylglycerol lipase beta OS=Cucurbita maxima OX=3661 GN=LOC111498003 PE=4 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.5e-239
Identity = 443/556 (79.68%), Postives = 486/556 (87.41%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQEWQAS--FVNSVLMHFQRSKADKVEIGVHKEGEVLPE 60
           M+NGTARRLL N + ARS+LRKQ+WQAS   VNSVLMH QRSKAD VE  V KE EVLPE
Sbjct: 1   MSNGTARRLLRNLYSARSYLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTG 120
           VS+RSEMEK  K GS+  K  SD EDISD +RRLELAWLTKAL+ A QL RWALSS   G
Sbjct: 61  VSVRSEMEKNIKKGSKTPKYFSDAEDISDSNRRLELAWLTKALKSASQLYRWALSS---G 120

Query: 121 GGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQ 180
            GI+NQ  PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL+LIYLRQASTNP  DIKGVQ
Sbjct: 121 DGIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQ 180

Query: 181 ISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTR 240
           I+S+AIV+DLIYYLELAKG YKDSAA LARN+MLRE NILKFI+DSSVMRPGYYIGVDTR
Sbjct: 181 ITSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLE 300
           KKLVIFGIRGTH+VYDLITDI TSSD EVTFEGYSTHFGT+ESARWFL+HE+ MIR+CL+
Sbjct: 241 KKLVIFGIRGTHSVYDLITDITTSSDEEVTFEGYSTHFGTSESARWFLDHEIEMIRQCLK 300

Query: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESC 360
           KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGF PDIVSAIG+ATPPCVS KLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFRPDIVSAIGFATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSV 420
           ADYVTTVVMQDDIIPRLS+ASLT+LR EILQTDW SVIE++DWK+   L+TNAKQVV+SV
Sbjct: 361 ADYVTTVVMQDDIIPRLSLASLTKLRIEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTSV 420

Query: 421 QDVTRKLADYAKFTNKKRSTNGSIRKQSHVASG-APSHPTA-------AQNEAARCKIPE 480
           QDV RKL DYAKFT+KK  ++ S  K SHVASG  PS PTA       AQNEAARCKIPE
Sbjct: 421 QDVARKLGDYAKFTSKKGPSDDSYGKGSHVASGPPPSQPTAVVQRADTAQNEAARCKIPE 480

Query: 481 ELFVPGTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALR 540
           ELFVPGTVYYLK++++ +   A EYFTLWKRHPDEHFQ+IVLSSNLISDH+CDSHY+ALR
Sbjct: 481 ELFVPGTVYYLKKHVNLEE--AYEYFTLWKRHPDEHFQQIVLSSNLISDHKCDSHYHALR 540

Query: 541 DALKGLPARNDEGIFT 547
           D LKGLP+ NDEGIF+
Sbjct: 541 DVLKGLPSCNDEGIFS 551

BLAST of Sgr019344 vs. ExPASy TrEMBL
Match: A0A1S3BGF8 (uncharacterized protein LOC103489737 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489737 PE=4 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 3.7e-230
Identity = 423/547 (77.33%), Postives = 475/547 (86.84%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQEWQAS--FVNSVLMHFQRSKADKVEIGVHKEGEVLPE 60
           MA GTARRL+HNFHYAR+FLRKQEWQA    VN+VLMHFQ SK DKVE  V+KEGEVL E
Sbjct: 1   MATGTARRLIHNFHYARNFLRKQEWQARSVTVNNVLMHFQSSKVDKVENDVNKEGEVLRE 60

Query: 61  VSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTG 120
           VSLRSEM K S I     K S   ED +D + R ELAWLTKALEPALQL RWALSS   G
Sbjct: 61  VSLRSEMGKNSTI---DCKRSEYVEDTTDSNWRSELAWLTKALEPALQLYRWALSS---G 120

Query: 121 GGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQ 180
            GI +    RSRSVSEIIASIQRSK GIQDWSLSDLTIGL LIYLRQASTNP ED+KGVQ
Sbjct: 121 DGIPH----RSRSVSEIIASIQRSKTGIQDWSLSDLTIGLCLIYLRQASTNPLEDLKGVQ 180

Query: 181 ISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTR 240
           ISS+AIV+DLIY++ELA+GSYK+S + LA+ +MLRECNILKF+++SSVMRPGYYIGVDTR
Sbjct: 181 ISSNAIVEDLIYFVELAEGSYKNSTSMLAKTTMLRECNILKFVKNSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLE 300
           KKLVIFGIRGTHTVYDLITDI+T+SDR+VTFEGYSTHFGT+ESA+WFL++E+GMIRRCLE
Sbjct: 241 KKLVIFGIRGTHTVYDLITDIITTSDRDVTFEGYSTHFGTSESAQWFLQNEIGMIRRCLE 300

Query: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESC 360
           KYQGFRLRLVGHSLGGAIASLLAVMLRKKS KELGFSPDIVSAIG+ TPPCVS KLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSKKELGFSPDIVSAIGFGTPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSV 420
           ADYVTTVVMQDD+IPRLS+ASLTRLR EILQTDWMS+I KEDWKS IDLVTNAKQVV+SV
Sbjct: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSLIGKEDWKSIIDLVTNAKQVVTSV 420

Query: 421 QDVTRKLADYAKFTNKKRSTNGSIRKQSHVASGAP--------SHPTAAQNEAARCKIPE 480
           QDV +KLADYAKFT+KK+S++ + +K+S VASG+P           TAAQN+AARCKI +
Sbjct: 421 QDVAQKLADYAKFTSKKKSSDDNNKKESDVASGSPRSCATSALQSATAAQNKAARCKISD 480

Query: 481 ELFVPGTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALR 538
           ELF+PGTVYYLKR++D      PEYF+LWKRHPDEHFQ+IVLS+ L+SDH+CDSHYYALR
Sbjct: 481 ELFIPGTVYYLKRHVD----STPEYFSLWKRHPDEHFQQIVLSNILLSDHKCDSHYYALR 533

BLAST of Sgr019344 vs. ExPASy TrEMBL
Match: A0A1S3BHV5 (uncharacterized protein LOC103489737 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489737 PE=4 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 3.7e-230
Identity = 423/547 (77.33%), Postives = 475/547 (86.84%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQEWQAS--FVNSVLMHFQRSKADKVEIGVHKEGEVLPE 60
           MA GTARRL+HNFHYAR+FLRKQEWQA    VN+VLMHFQ SK DKVE  V+KEGEVL E
Sbjct: 1   MATGTARRLIHNFHYARNFLRKQEWQARSVTVNNVLMHFQSSKVDKVENDVNKEGEVLRE 60

Query: 61  VSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALSSGGTG 120
           VSLRSEM K S I     K S   ED +D + R ELAWLTKALEPALQL RWALSS   G
Sbjct: 61  VSLRSEMGKNSTI---DCKRSEYVEDTTDSNWRSELAWLTKALEPALQLYRWALSS---G 120

Query: 121 GGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDIKGVQ 180
            GI +    RSRSVSEIIASIQRSK GIQDWSLSDLTIGL LIYLRQASTNP ED+KGVQ
Sbjct: 121 DGIPH----RSRSVSEIIASIQRSKTGIQDWSLSDLTIGLCLIYLRQASTNPLEDLKGVQ 180

Query: 181 ISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTR 240
           ISS+AIV+DLIY++ELA+GSYK+S + LA+ +MLRECNILKF+++SSVMRPGYYIGVDTR
Sbjct: 181 ISSNAIVEDLIYFVELAEGSYKNSTSMLAKTTMLRECNILKFVKNSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMIRRCLE 300
           KKLVIFGIRGTHTVYDLITDI+T+SDR+VTFEGYSTHFGT+ESA+WFL++E+GMIRRCLE
Sbjct: 241 KKLVIFGIRGTHTVYDLITDIITTSDRDVTFEGYSTHFGTSESAQWFLQNEIGMIRRCLE 300

Query: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESC 360
           KYQGFRLRLVGHSLGGAIASLLAVMLRKKS KELGFSPDIVSAIG+ TPPCVS KLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSKKELGFSPDIVSAIGFGTPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQVVSSV 420
           ADYVTTVVMQDD+IPRLS+ASLTRLR EILQTDWMS+I KEDWKS IDLVTNAKQVV+SV
Sbjct: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSLIGKEDWKSIIDLVTNAKQVVTSV 420

Query: 421 QDVTRKLADYAKFTNKKRSTNGSIRKQSHVASGAP--------SHPTAAQNEAARCKIPE 480
           QDV +KLADYAKFT+KK+S++ + +K+S VASG+P           TAAQN+AARCKI +
Sbjct: 421 QDVAQKLADYAKFTSKKKSSDDNNKKESDVASGSPRSCATSALQSATAAQNKAARCKISD 480

Query: 481 ELFVPGTVYYLKRNMDDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYALR 538
           ELF+PGTVYYLKR++D      PEYF+LWKRHPDEHFQ+IVLS+ L+SDH+CDSHYYALR
Sbjct: 481 ELFIPGTVYYLKRHVD----STPEYFSLWKRHPDEHFQQIVLSNILLSDHKCDSHYYALR 533

BLAST of Sgr019344 vs. TAIR 10
Match: AT2G42450.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 625.2 bits (1611), Expect = 5.1e-179
Identity = 332/556 (59.71%), Postives = 412/556 (74.10%), Query Frame = 0

Query: 1   MANGTARRLLHNFHYARSFLRKQE-WQASFVNSVLMHFQR--SKADKVEIGVHKE----G 60
           MAN    RLL NF    S  RK+       V +V+  FQ   S++ KVE  + ++     
Sbjct: 1   MANRALNRLLRNFFVESSPFRKRSGLYQGVVYNVIKRFQSHLSESGKVEKELEEQEAEKR 60

Query: 61  EVLPEVSLRSEMEKKSKIGSEPSKNSSDEEDISDCSRRLELAWLTKALEPALQLCRWALS 120
                 S RS +EK+ +  S    +SS +ED  D   +L+LAWLTKALEPA+QL RWAL 
Sbjct: 61  RPSSVTSSRSGLEKRRQFESGIDNDSSSDEDTGDGKWKLDLAWLTKALEPAMQLRRWALP 120

Query: 121 SGGTGGGIRNQTAPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLYLIYLRQASTNPFED 180
           +G        ++ P SRS+SEIIASIQRS++GI+ W+  DLTIGLYLIYLRQAS +PFED
Sbjct: 121 TG-------EKSLPGSRSLSEIIASIQRSRLGIEGWTFGDLTIGLYLIYLRQASLSPFED 180

Query: 181 IKGVQISSDAIVQDLIYYLELAKGSYKDSAATLARNSMLRECNILKFIRDSSVMRPGYYI 240
           +KGV++ S++ V DLIY  ELAKG Y+DS + LA+N+MLRE NILKF++DSSVMRPGYYI
Sbjct: 181 VKGVEVVSESTVYDLIYNAELAKGCYRDSVSGLAKNTMLRENNILKFVKDSSVMRPGYYI 240

Query: 241 GVDTRKKLVIFGIRGTHTVYDLITDIVTSSDREVTFEGYSTHFGTAESARWFLEHEMGMI 300
           GVD R+KLV+FGIRGTHT+YDLITDIV+SSD EVTFEGYSTHFGTAE+ARWFL HE+  I
Sbjct: 241 GVDHRRKLVVFGIRGTHTIYDLITDIVSSSDEEVTFEGYSTHFGTAEAARWFLNHELQTI 300

Query: 301 RRCLEKYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIK 360
           RRCL KY+G++LRLVGHSLGGAIASL+A+ML+K   +ELGF  +I+SA+GYATPPCVS +
Sbjct: 301 RRCLAKYEGYKLRLVGHSLGGAIASLMAIMLKKMPREELGFDAEIISAVGYATPPCVSKE 360

Query: 361 LAESCADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWMSVIEKEDWKSFIDLVTNAKQ 420
           LAE+C+++VTT+VMQDDIIPRLS ASL RLRNEILQTDW SVIEKE+WK+ +DLVTNAKQ
Sbjct: 361 LAENCSEFVTTIVMQDDIIPRLSAASLARLRNEILQTDWTSVIEKEEWKNVLDLVTNAKQ 420

Query: 421 VVSSVQDVTRKLADYAKFTNKKRSTNGSIRKQSHVASGAPSHPTAAQNEAARCKIPEELF 480
           VV+SVQDV RK++DYA F NKK      I    +  SG     +  ++     K+PEEL+
Sbjct: 421 VVTSVQDVARKVSDYANFGNKKEVP--EIPSSKNNQSGILISDSTTKDVV---KLPEELY 480

Query: 481 VPGTVYYLKRNM----DDQRGGAPEYFTLWKRHPDEHFQRIVLSSNLISDHRCDSHYYAL 540
           VPG VYYL R++        G   EY++LWKR P +HFQRI+LS N I+DH+CDSHYYAL
Sbjct: 481 VPGAVYYLMRSLRGTPKSSAGKQVEYYSLWKRDPGQHFQRILLSGNFITDHKCDSHYYAL 540

Query: 541 RDALKGLPARNDEGIF 546
           RD LKG P+  +E IF
Sbjct: 541 RDVLKGFPSFINESIF 544

BLAST of Sgr019344 vs. TAIR 10
Match: AT4G16070.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 102.1 bits (253), Expect = 1.5e-21
Identity = 73/263 (27.76%), Postives = 129/263 (49.05%), Query Frame = 0

Query: 149 WSLSDLTIGLYLIYLRQ---------ASTNPFEDIKGVQISSDAIVQDLIYYLELAKGSY 208
           W ++DL  G+  +  RQ         A +N  E +KG +I  D  + +L+ +L L     
Sbjct: 81  WPIADLAFGINYLMRRQGNFPTASVYAGSNCIE-LKGPEIIMD--LTELLRFLTLCMLFS 140

Query: 209 KDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTRKKLVIFGIRGTHTVYDLI--- 268
           K   A    ++     ++L     + +M+P + I  DT  K ++  IRGTH++ D +   
Sbjct: 141 KKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNSKCILLLIRGTHSIKDTLTAA 200

Query: 269 TDIVTSSDREVTFEGYST-------HFGTAESARWFLEHEMGMIRRCLEKYQGFRLRLVG 328
           T  V      V  +G  +       H G   +ARW  +  +  + + L++   F++++VG
Sbjct: 201 TGAVVPFHHSVLHDGGLSNLVLGYAHCGMVAAARWIAKLSVPCLLKALDENPSFKVQIVG 260

Query: 329 HSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESCADYVTTVVMQD 388
           HSLGG  ASLL  +LR++  KE   +    +   +A   C++  LAES   ++TT++   
Sbjct: 261 HSLGGGTASLLTYILREQ--KEFASA----TCFTFAPAACMTWDLAESGKHFITTIINGS 320

Query: 389 DIIPRLSIASLTRLRNEILQTDW 393
           D++P  S +S+  LR+E+  + W
Sbjct: 321 DLVPTFSASSVDDLRSEVTSSSW 334

BLAST of Sgr019344 vs. TAIR 10
Match: AT4G16070.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 98.6 bits (244), Expect = 1.6e-20
Identity = 70/263 (26.62%), Postives = 124/263 (47.15%), Query Frame = 0

Query: 149 WSLSDLTIGLYLIYLRQ---------ASTNPFEDIKGVQISSDAIVQDLIYYLELAKGSY 208
           W ++DL  G+  +  RQ         A +N  E +KG +I  D  + +L+ +L L     
Sbjct: 81  WPIADLAFGINYLMRRQGNFPTASVYAGSNCIE-LKGPEIIMD--LTELLRFLTLCMLFS 140

Query: 209 KDSAATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTRKKLVIFGIRGTHTVYDLI--- 268
           K   A    ++     ++L     + +M+P + I  DT  K ++  IRGTH++ D +   
Sbjct: 141 KKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNSKCILLLIRGTHSIKDTLTAA 200

Query: 269 TDIVTSSDREVTFEGYST-------HFGTAESARWFLEHEMGMIRRCLEKYQGFRLRLVG 328
           T  V      V  +G  +       H G   +ARW  +  +  + + L++   F++++VG
Sbjct: 201 TGAVVPFHHSVLHDGGLSNLVLGYAHCGMVAAARWIAKLSVPCLLKALDENPSFKVQIVG 260

Query: 329 HSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESCADYVTTVVMQD 388
           HSLGG  ASLL  +LR++                +A+  C +   AES   ++TT++   
Sbjct: 261 HSLGGGTASLLTYILREQKE--------------FASATCFTFAPAESGKHFITTIINGS 320

Query: 389 DIIPRLSIASLTRLRNEILQTDW 393
           D++P  S +S+  LR+E+  + W
Sbjct: 321 DLVPTFSASSVDDLRSEVTSSSW 326

BLAST of Sgr019344 vs. TAIR 10
Match: AT3G14075.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 90.9 bits (224), Expect = 3.4e-18
Identity = 65/267 (24.34%), Postives = 123/267 (46.07%), Query Frame = 0

Query: 149 WSLSDLTIGLYLIYLRQASTN---PFEDIKGVQISSDAIVQDLIYYLELAKGSY---KDS 208
           W + DL  G+  +  RQ   +    F     V++    +  +L Y L L    +   K S
Sbjct: 83  WPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGSEVATELKYLLHLLTLCWHFSKKS 142

Query: 209 AATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTRKKLVIFGIRGTHTVYDLIT----D 268
                  +   + N+L     + +++P + + VD   K  +  IRGTH++ D +T     
Sbjct: 143 FPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNTKYFLLLIRGTHSIKDTLTAATGA 202

Query: 269 IVTSSDREVTFEGYS------THFGTAESARWFLEHEMGMIRRCLEKYQGFRLRLVGHSL 328
           IV      V   G S       H G   +AR   +     + + LE+Y  +++++VGHSL
Sbjct: 203 IVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLATPCLLKGLEQYPDYKIKIVGHSL 262

Query: 329 GGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESCADYVTTVVMQDDII 388
           GG  A+LL  ++R++            + + +A   C++ +LA+S  D++ +V+   D++
Sbjct: 263 GGGTAALLTYIMREQK------MLSTATCVTFAPAACMTWELADSGNDFIVSVINGADLV 322

Query: 389 PRLSIASLTRLRNEILQTDWMSVIEKE 400
           P  S A++  LR E+  + W++ +  +
Sbjct: 323 PTFSAAAVDDLRAEVTASAWLNDLRNQ 343

BLAST of Sgr019344 vs. TAIR 10
Match: AT3G14075.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 90.9 bits (224), Expect = 3.4e-18
Identity = 65/267 (24.34%), Postives = 123/267 (46.07%), Query Frame = 0

Query: 149 WSLSDLTIGLYLIYLRQASTN---PFEDIKGVQISSDAIVQDLIYYLELAKGSY---KDS 208
           W + DL  G+  +  RQ   +    F     V++    +  +L Y L L    +   K S
Sbjct: 83  WPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGSEVATELKYLLHLLTLCWHFSKKS 142

Query: 209 AATLARNSMLRECNILKFIRDSSVMRPGYYIGVDTRKKLVIFGIRGTHTVYDLIT----D 268
                  +   + N+L     + +++P + + VD   K  +  IRGTH++ D +T     
Sbjct: 143 FPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNTKYFLLLIRGTHSIKDTLTAATGA 202

Query: 269 IVTSSDREVTFEGYS------THFGTAESARWFLEHEMGMIRRCLEKYQGFRLRLVGHSL 328
           IV      V   G S       H G   +AR   +     + + LE+Y  +++++VGHSL
Sbjct: 203 IVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLATPCLLKGLEQYPDYKIKIVGHSL 262

Query: 329 GGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSIKLAESCADYVTTVVMQDDII 388
           GG  A+LL  ++R++            + + +A   C++ +LA+S  D++ +V+   D++
Sbjct: 263 GGGTAALLTYIMREQK------MLSTATCVTFAPAACMTWELADSGNDFIVSVINGADLV 322

Query: 389 PRLSIASLTRLRNEILQTDWMSVIEKE 400
           P  S A++  LR E+  + W++ +  +
Sbjct: 323 PTFSAAAVDDLRAEVTASAWLNDLRNQ 343

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022156622.12.9e-26187.30uncharacterized protein LOC111023479 [Momordica charantia][more]
XP_023513693.11.5e-24180.40sn1-specific diacylglycerol lipase beta [Cucurbita pepo subsp. pepo][more]
XP_022959891.11.3e-24080.22sn1-specific diacylglycerol lipase beta [Cucurbita moschata][more]
XP_023004816.13.1e-23979.68sn1-specific diacylglycerol lipase beta [Cucurbita maxima][more]
XP_038897252.15.3e-23979.34uncharacterized protein LOC120085373 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P0C1S95.5e-1325.81Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1[more]
Q8NCG72.7e-1223.51Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2[more]
Q91WC94.7e-1223.24Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2[more]
Q9Y4D21.8e-0826.94Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3[more]
Q6WQJ11.8e-0826.94Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1DSG21.4e-26187.30uncharacterized protein LOC111023479 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1H6496.2e-24180.22sn1-specific diacylglycerol lipase beta OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KT651.5e-23979.68sn1-specific diacylglycerol lipase beta OS=Cucurbita maxima OX=3661 GN=LOC111498... [more]
A0A1S3BGF83.7e-23077.33uncharacterized protein LOC103489737 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3BHV53.7e-23077.33uncharacterized protein LOC103489737 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT2G42450.15.1e-17959.71alpha/beta-Hydrolases superfamily protein [more]
AT4G16070.11.5e-2127.76Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT4G16070.21.6e-2026.62Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT3G14075.13.4e-1824.34Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT3G14075.23.4e-1824.34Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 244..377
e-value: 2.9E-23
score: 82.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 174..434
e-value: 1.6E-35
score: 124.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 187..377
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 64..84
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 437..457
NoneNo IPR availablePANTHERPTHR47418ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 95..541
NoneNo IPR availableCDDcd00519Lipase_3coord: 188..376
e-value: 1.33707E-38
score: 139.534

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr019344.1Sgr019344.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016787 hydrolase activity