Sgr019213 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr019213
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionCBL-interacting serine/threonine-protein kinase 12
Locationtig00153293: 721671 .. 723071 (+)
RNA-Seq ExpressionSgr019213
SyntenySgr019213
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGACCGGCCGGAACCCGACAAGAAGAACGAGAACAGCAAGAAGGAGATGCCGGCTCTTCTTTTGGGTCGATACGAGATCGGAAAGCTTCTCGGCCATGGCACCTTTGCCAAGGTCTACCACGCCCGAAACATCAAAACAAACGAAAGCGTGGCCATTAAAGTCATCGACAAGGAGAAGATCCTCAAGGGCGGTCTAATCGCTCACATCAAGCGTGAGATCTCAATCCTTCGCCGCGTTCGCCACCCCAACATCGTACAACTCTTCGAGGTTATGGCCACGAAGGCCAAGATCTACTTCGTTATGGAATATGTTCGCGGCGGCGAGCTCTTCAAGAAGGTCTCCAAGGGCCGATTGAAGGAAGAGGTCGCACGAAAATACTTCCAACAATTAATCTCAGCCGTTGCCTTCTGCCACGCTCGCGGTGTTTATCATCGCGACCTTAAGCCGGAGAATCTCTTGCTCGACGAAAATGGCAACCTCAAGGTCTCCGATTTCGGCCTCAGCGCCGTCTCCGATCAAATTCAACAAGACGGTTTGTTTCACACTTTTTGTGGTACACCAGCCTATGTTGCTCCTGAGGTTTTGGCCCGAAAGGGCTATGAAGCCGCTAAGGTAGATATTTGGTCCTGTGGGGTTATCCTCTTCGTTCTGATGGCTGGTTATTTACCTTTCCACGACCAAAATATCATGGCCATGTATAAGAAAATTTACAAGGGCGAGTTCCGGTGTCCGAGATGGTTCTCGCCGGAGCTAATTCGACTCCTCTCTCGCCTTTTAGATACAAACCCCGAAACCCGATTCACCATTCCTGAAATCATGGAGAATAAATGGTTCAAAAAGGGGTATAAGCATATCAAATTCTACATCGAAGACGATAAGCTCTGCAGCGTCGAAGATGACAACGACGACGTCGATTCCTTGTCGGATCAATCGCAATCGGAATCCGATTCCGAAATCGAGACTCGGAGAAAGGTCGCTTCACTCCCAAGACCGGCGAGTCTGAATGCATTTGATATTATTTCCTTTTCGCCAGGATTTGACCTTTCTGGGTTGTTCGAGGACGGAGGAGAAGAGGCCAGATTTGTGTCCGGCGCTCCGGTGTCGAAGATTATATCGAAACTGGAGGAGATTGCAAAATTGGTGAGCTTTACCGTGAGGAAGAAGGATTGCAGAGTGAGTTTAGAGGGCTCACGGGAAGGTGTAAAAGGGCCATTAACGATCGCCGCCGAGATATTCGAGTTGACGCCGAAGTTGGTGATGGTGGAGGTGAAGAGGAAAGGAGGAGATAAAGCAGAGTACGAACAGTTCTGCAATAAGGAATTGAAACCAGCATTGCAGAAGCTGAAGATGGAGGAATCTGCTGCTCCATCACAAATACCTTCCGACGCTGAATAA

mRNA sequence

ATGGCCGACCGGCCGGAACCCGACAAGAAGAACGAGAACAGCAAGAAGGAGATGCCGGCTCTTCTTTTGGGTCGATACGAGATCGGAAAGCTTCTCGGCCATGGCACCTTTGCCAAGGTCTACCACGCCCGAAACATCAAAACAAACGAAAGCGTGGCCATTAAAGTCATCGACAAGGAGAAGATCCTCAAGGGCGGTCTAATCGCTCACATCAAGCGTGAGATCTCAATCCTTCGCCGCGTTCGCCACCCCAACATCGTACAACTCTTCGAGGTTATGGCCACGAAGGCCAAGATCTACTTCGTTATGGAATATGTTCGCGGCGGCGAGCTCTTCAAGAAGGTCTCCAAGGGCCGATTGAAGGAAGAGGTCGCACGAAAATACTTCCAACAATTAATCTCAGCCGTTGCCTTCTGCCACGCTCGCGGTGTTTATCATCGCGACCTTAAGCCGGAGAATCTCTTGCTCGACGAAAATGGCAACCTCAAGGTCTCCGATTTCGGCCTCAGCGCCGTCTCCGATCAAATTCAACAAGACGGTTTGTTTCACACTTTTTGTGGTACACCAGCCTATGTTGCTCCTGAGGTTTTGGCCCGAAAGGGCTATGAAGCCGCTAAGGTAGATATTTGGTCCTGTGGGGTTATCCTCTTCGTTCTGATGGCTGGTTATTTACCTTTCCACGACCAAAATATCATGGCCATGTATAAGAAAATTTACAAGGGCGAGTTCCGGTGTCCGAGATGGTTCTCGCCGGAGCTAATTCGACTCCTCTCTCGCCTTTTAGATACAAACCCCGAAACCCGATTCACCATTCCTGAAATCATGGAGAATAAATGGTTCAAAAAGGGGTATAAGCATATCAAATTCTACATCGAAGACGATAAGCTCTGCAGCGTCGAAGATGACAACGACGACGTCGATTCCTTGTCGGATCAATCGCAATCGGAATCCGATTCCGAAATCGAGACTCGGAGAAAGGTCGCTTCACTCCCAAGACCGGCGAGTCTGAATGCATTTGATATTATTTCCTTTTCGCCAGGATTTGACCTTTCTGGGTTGTTCGAGGACGGAGGAGAAGAGGCCAGATTTGTGTCCGGCGCTCCGGTGTCGAAGATTATATCGAAACTGGAGGAGATTGCAAAATTGGTGAGCTTTACCGTGAGGAAGAAGGATTGCAGAGTGAGTTTAGAGGGCTCACGGGAAGGTGTAAAAGGGCCATTAACGATCGCCGCCGAGATATTCGAGTTGACGCCGAAGTTGGTGATGGTGGAGGTGAAGAGGAAAGGAGGAGATAAAGCAGAGTACGAACAGTTCTGCAATAAGGAATTGAAACCAGCATTGCAGAAGCTGAAGATGGAGGAATCTGCTGCTCCATCACAAATACCTTCCGACGCTGAATAA

Coding sequence (CDS)

ATGGCCGACCGGCCGGAACCCGACAAGAAGAACGAGAACAGCAAGAAGGAGATGCCGGCTCTTCTTTTGGGTCGATACGAGATCGGAAAGCTTCTCGGCCATGGCACCTTTGCCAAGGTCTACCACGCCCGAAACATCAAAACAAACGAAAGCGTGGCCATTAAAGTCATCGACAAGGAGAAGATCCTCAAGGGCGGTCTAATCGCTCACATCAAGCGTGAGATCTCAATCCTTCGCCGCGTTCGCCACCCCAACATCGTACAACTCTTCGAGGTTATGGCCACGAAGGCCAAGATCTACTTCGTTATGGAATATGTTCGCGGCGGCGAGCTCTTCAAGAAGGTCTCCAAGGGCCGATTGAAGGAAGAGGTCGCACGAAAATACTTCCAACAATTAATCTCAGCCGTTGCCTTCTGCCACGCTCGCGGTGTTTATCATCGCGACCTTAAGCCGGAGAATCTCTTGCTCGACGAAAATGGCAACCTCAAGGTCTCCGATTTCGGCCTCAGCGCCGTCTCCGATCAAATTCAACAAGACGGTTTGTTTCACACTTTTTGTGGTACACCAGCCTATGTTGCTCCTGAGGTTTTGGCCCGAAAGGGCTATGAAGCCGCTAAGGTAGATATTTGGTCCTGTGGGGTTATCCTCTTCGTTCTGATGGCTGGTTATTTACCTTTCCACGACCAAAATATCATGGCCATGTATAAGAAAATTTACAAGGGCGAGTTCCGGTGTCCGAGATGGTTCTCGCCGGAGCTAATTCGACTCCTCTCTCGCCTTTTAGATACAAACCCCGAAACCCGATTCACCATTCCTGAAATCATGGAGAATAAATGGTTCAAAAAGGGGTATAAGCATATCAAATTCTACATCGAAGACGATAAGCTCTGCAGCGTCGAAGATGACAACGACGACGTCGATTCCTTGTCGGATCAATCGCAATCGGAATCCGATTCCGAAATCGAGACTCGGAGAAAGGTCGCTTCACTCCCAAGACCGGCGAGTCTGAATGCATTTGATATTATTTCCTTTTCGCCAGGATTTGACCTTTCTGGGTTGTTCGAGGACGGAGGAGAAGAGGCCAGATTTGTGTCCGGCGCTCCGGTGTCGAAGATTATATCGAAACTGGAGGAGATTGCAAAATTGGTGAGCTTTACCGTGAGGAAGAAGGATTGCAGAGTGAGTTTAGAGGGCTCACGGGAAGGTGTAAAAGGGCCATTAACGATCGCCGCCGAGATATTCGAGTTGACGCCGAAGTTGGTGATGGTGGAGGTGAAGAGGAAAGGAGGAGATAAAGCAGAGTACGAACAGTTCTGCAATAAGGAATTGAAACCAGCATTGCAGAAGCTGAAGATGGAGGAATCTGCTGCTCCATCACAAATACCTTCCGACGCTGAATAA

Protein sequence

MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDGLFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVEDDNDDVDSLSDQSQSESDSEIETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGEEARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPKLVMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESAAPSQIPSDAE
Homology
BLAST of Sgr019213 vs. NCBI nr
Match: XP_038882513.1 (CBL-interacting serine/threonine-protein kinase 12-like [Benincasa hispida])

HSP 1 Score: 880.9 bits (2275), Expect = 4.7e-252
Identity = 444/467 (95.07%), Postives = 457/467 (97.86%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRP+PDKK E ++K+MPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMEN+WFKKGYKHIKFYIEDDK+CSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSQSESDSEI-ETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDN+DVDSLSDQSQSESDSEI ETRRKV SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNNDVDSLSDQSQSESDSEIFETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPK 420
           EARFVS APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGV+GPLTIAAE+FELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVRGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESAAPSQIPSDAE 467
           LVMVEVKRKGGDKAEYEQFCN ELKPAL  LK+E+SAAPS IPSD E
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSDTE 467

BLAST of Sgr019213 vs. NCBI nr
Match: XP_008439731.1 (PREDICTED: CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo] >KAA0052614.1 CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo var. makuwa] >TYK13214.1 CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo var. makuwa])

HSP 1 Score: 880.2 bits (2273), Expect = 8.0e-252
Identity = 444/467 (95.07%), Postives = 455/467 (97.43%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRP+PDKK E ++K+MPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMEN+WFKKGYKHIKFYIEDDK+CSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSQSESDSE-IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDNDDVDSLSDQSQSESDSE IETRRKV SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPK 420
           EARFVS APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAE+FELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESAAPSQIPSDAE 467
           LVMVEVKRKGGDKAEYEQFCN ELKPAL  LK+E+S  PS IPSD E
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467

BLAST of Sgr019213 vs. NCBI nr
Match: XP_011658253.1 (CBL-interacting serine/threonine-protein kinase 12 [Cucumis sativus] >KGN49312.1 hypothetical protein Csa_003581 [Cucumis sativus])

HSP 1 Score: 874.4 bits (2258), Expect = 4.4e-250
Identity = 440/467 (94.22%), Postives = 453/467 (97.00%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRP+PDKK E ++K+MPALLLGRYEIGKLLGHGTFAKVYHARNIKTN+SVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNDSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMEN+WFKKGYKHIKFYIEDDK+CS+E
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSIE 300

Query: 301 DDNDDVDSLSDQSQSESDSE-IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DD DDVDSLSDQSQSESDSE IETRRKV SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDTDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPK 420
           EARFVS APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAE+FELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESAAPSQIPSDAE 467
           LVMVEVKRKGGDK EYEQFCN ELKPAL  LK+E+S  PS IPSD E
Sbjct: 421 LVMVEVKRKGGDKEEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467

BLAST of Sgr019213 vs. NCBI nr
Match: XP_022142362.1 (CBL-interacting serine/threonine-protein kinase 12-like [Momordica charantia])

HSP 1 Score: 868.2 bits (2242), Expect = 3.1e-248
Identity = 439/474 (92.62%), Postives = 453/474 (95.57%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRP+PDKK +++KK+MPALLLGRYEIGKLLGHGTFAKVYHARNIKT+ESVAIKVIDKE
Sbjct: 1   MADRPDPDKKPDSNKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEA KVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAVKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFSPEL+RLL RLLDTNPETR TIPEIMEN+WFKKGYKHIKFYIEDDK+CSV+
Sbjct: 241 GEFRCPRWFSPELVRLLYRLLDTNPETRITIPEIMENRWFKKGYKHIKFYIEDDKVCSVQ 300

Query: 301 DDNDDVDSLSDQSQSESDSEIETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGEE 360
           +DNDD+DSLSDQSQSESDSEIE RRK ASLPRPASLNAFDIISFSPGFDLSGLFEDGGEE
Sbjct: 301 EDNDDIDSLSDQSQSESDSEIEFRRKTASLPRPASLNAFDIISFSPGFDLSGLFEDGGEE 360

Query: 361 ARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPKL 420
           ARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGS EGVKGPLTIAAEIFELTPKL
Sbjct: 361 ARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSMEGVKGPLTIAAEIFELTPKL 420

Query: 421 VMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESA--------APSQIPSDAE 467
           VMVEVKRKGGDKAEYE FCN ELKPALQ LKMEE +        APS IPSD E
Sbjct: 421 VMVEVKRKGGDKAEYEHFCNVELKPALQNLKMEEDSAAAPAPAPAPSHIPSDTE 474

BLAST of Sgr019213 vs. NCBI nr
Match: XP_022926641.1 (CBL-interacting serine/threonine-protein kinase 12-like [Cucurbita moschata] >KAG6594541.1 CBL-interacting serine/threonine-protein kinase 12, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026520.1 CBL-interacting serine/threonine-protein kinase 12, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 848.6 bits (2191), Expect = 2.6e-242
Identity = 436/474 (91.98%), Postives = 453/474 (95.57%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRP+ DKK E +KK+MPALLLGRYEIGKLLGHGTFAKVYHARNIKT++SVAIKVIDKE
Sbjct: 1   MADRPDTDKKTETTKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDQSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAVAFCHARGV+HRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVFHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVL RKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLTRKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMEN+WFKKGYKHIKFYIEDDK+CSVE
Sbjct: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDV---DSLSDQSQSESDSE-IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFED 360
           DD +DV   DS SDQSQSESDSE IETRRKV++LPRPASLNAFDIISFSPGFDLSGLFED
Sbjct: 301 DDQNDVDIIDSSSDQSQSESDSEIIETRRKVSTLPRPASLNAFDIISFSPGFDLSGLFED 360

Query: 361 GGEEARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFEL 420
           GGEEARFVS APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAE+FEL
Sbjct: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420

Query: 421 TPKLVMVEVKRKGGDKAEYEQFCNKELKPALQKLKME---ESAAP-SQIPSDAE 467
           T KLVMVEVKRKGGDKAEYEQFCN+ELKPAL  LK+E    +AAP S IPSD E
Sbjct: 421 TQKLVMVEVKRKGGDKAEYEQFCNEELKPALLNLKVEVEDSAAAPSSHIPSDTE 474

BLAST of Sgr019213 vs. ExPASy Swiss-Prot
Match: Q9SN43 (CBL-interacting serine/threonine-protein kinase 12 OS=Arabidopsis thaliana OX=3702 GN=CIPK12 PE=1 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 2.5e-216
Identity = 380/462 (82.25%), Postives = 413/462 (89.39%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MA++   +      +    AL+LGRYE+GKLLGHGTFAKVY ARN+KTNESVAIKVIDKE
Sbjct: 1   MAEKITRETSLPKERSSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           K+LKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELF KV+KGRL
Sbjct: 61  KVLKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGY+AAKVDIWSCGVILFVLMAGYLPFHD+N+MAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFS EL RLLS+LL+TNPE RFT PEIMEN WFKKG+KHIKFY+EDDKLC+V 
Sbjct: 241 GEFRCPRWFSTELTRLLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVV 300

Query: 301 DD----NDDVDSLSDQSQSESDSE-IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFE 360
           DD    +D V+S  D + SES+ E +E RR+V  LPRPASLNAFDIISFS GFDLSGLF+
Sbjct: 301 DDDELESDSVESDRDSAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFD 360

Query: 361 DGGEEARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFE 420
           D GE +RFVSGAPVSKIISKLEEIAK+VSFTVRKKDCRVSLEGSR+GVKGPLTIAAEIFE
Sbjct: 361 DDGEGSRFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFE 420

Query: 421 LTPKLVMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESAA 458
           LTP LV+VEVK+KGGDK EYE FCN ELKP LQ L  ++  A
Sbjct: 421 LTPSLVVVEVKKKGGDKTEYEDFCNNELKPKLQNLTADDVVA 462

BLAST of Sgr019213 vs. ExPASy Swiss-Prot
Match: Q9FJ55 (CBL-interacting serine/threonine-protein kinase 19 OS=Arabidopsis thaliana OX=3702 GN=CIPK19 PE=1 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 8.7e-201
Identity = 354/472 (75.00%), Postives = 410/472 (86.86%), Query Frame = 0

Query: 10  KNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIA 69
           K +  +    AL+LG+YE+G+LLGHGTFAKVY ARN ++ ESVAIKVIDKEK+LK GLIA
Sbjct: 12  KKKQDQSNHQALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVIDKEKVLKSGLIA 71

Query: 70  HIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYF 129
           HIKREISILRRVRHPNIVQLFEVMATK+KIYFVMEYV+GGELF KV+KGRLKEE+ARKYF
Sbjct: 72  HIKREISILRRVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKYF 131

Query: 130 QQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDGLFHTFCGTP 189
           QQLISAV+FCH RGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDGLFHTFCGTP
Sbjct: 132 QQLISAVSFCHFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP 191

Query: 190 AYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWF 249
           AYVAPEVLARKGY+ AKVDIWSCGVILFVLMAG+LPFHD+N+MAMYKKIY+G+FRCPRWF
Sbjct: 192 AYVAPEVLARKGYDGAKVDIWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWF 251

Query: 250 SPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDD-KLCSVEDDN--DDV 309
             E+ RLL R+L+T PE RFT+P+IME  WFKKG+KHIKFY+EDD +LC+V DD+  + +
Sbjct: 252 PVEINRLLIRMLETKPERRFTMPDIMETSWFKKGFKHIKFYVEDDHQLCNVADDDEIESI 311

Query: 310 DSLSDQSQSESDSE----IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGEEAR 369
           +S+S +S + S+ E     + RR+  S+PRPASLNAFD+ISFSPGFDLSGLFED GE +R
Sbjct: 312 ESVSGRSSTVSEPEDFESFDGRRRGGSMPRPASLNAFDLISFSPGFDLSGLFEDDGEGSR 371

Query: 370 FVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREG-VKGPLTIAAEIFELTPKLV 429
           FVSGAPV +IISKLEEIA++VSFTVRKKDC+VSLEGSREG +KGPL+IAAEIFELTP LV
Sbjct: 372 FVSGAPVGQIISKLEEIARIVSFTVRKKDCKVSLEGSREGSMKGPLSIAAEIFELTPALV 431

Query: 430 MVEVKRKGGDKAEYEQFCNKELKPALQKLKMEES-------AAPSQIPSDAE 467
           +VEVK+KGGDK EY++FCNKELKP LQ L  E         + PS + SD +
Sbjct: 432 VVEVKKKGGDKMEYDEFCNKELKPKLQNLSSENGQRVSGSRSLPSFLLSDTD 483

BLAST of Sgr019213 vs. ExPASy Swiss-Prot
Match: Q9LP51 (CBL-interacting serine/threonine-protein kinase 18 OS=Arabidopsis thaliana OX=3702 GN=CIPK18 PE=1 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 4.2e-195
Identity = 339/452 (75.00%), Postives = 394/452 (87.17%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           +L+G+YE+GKLLGHGTFAKVY A+NIK+ + VAIKVIDKEKI+K GL+AHIKREISILRR
Sbjct: 69  ILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKIMKSGLVAHIKREISILRR 128

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFCH 140
           VRHP IV LFEVMATK+KIYFVMEYV GGELF  V+KGRL EE AR+YFQQLIS+V+FCH
Sbjct: 129 VRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPEETARRYFQQLISSVSFCH 188

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDGLFHTFCGTPAYVAPEVLARK 200
            RGVYHRDLKPENLLLD  GNLKVSDFGLSAV++Q++QDGL HTFCGTPAY+APEVL RK
Sbjct: 189 GRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLCHTFCGTPAYIAPEVLTRK 248

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLSRL 260
           GY+AAK D+WSCGVILFVLMAG++PF+D+NIM MYKKIYKGEFRCPRWFS +L+RLL+RL
Sbjct: 249 GYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGEFRCPRWFSSDLVRLLTRL 308

Query: 261 LDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVEDDNDDVDSLSDQSQ--SESD 320
           LDTNP+TR TIPEIM+N+WFKKG+KH+KFYIEDDKLC  ++D ++  S S +S   SESD
Sbjct: 309 LDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDEDEEEEASSSGRSSTVSESD 368

Query: 321 SEIETRRK-VASLPRPASLNAFDIISFSPGFDLSGLF-EDGGEEARFVSGAPVSKIISKL 380
           +E + +R  + S+PRP+SLNAFDIISFS GFDLSGLF E+GGE  RFVSGAPVSKIISKL
Sbjct: 369 AEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEEEGGEGTRFVSGAPVSKIISKL 428

Query: 381 EEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPKLVMVEVKRKGGDKAEYE 440
           EEIAK+VSFTVRKK+  + LEG REG KGPLTIAAEIFELTP LV+VEVK+KGGD+ EYE
Sbjct: 429 EEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFELTPSLVVVEVKKKGGDREEYE 488

Query: 441 QFCNKELKPALQKLKMEESAAPS--QIPSDAE 467
           +FCNKEL+P L+KL  EE        +PSD E
Sbjct: 489 EFCNKELRPELEKLIHEEVVVEEALYLPSDTE 520

BLAST of Sgr019213 vs. ExPASy Swiss-Prot
Match: O22971 (CBL-interacting serine/threonine-protein kinase 13 OS=Arabidopsis thaliana OX=3702 GN=CIPK13 PE=1 SV=2)

HSP 1 Score: 636.0 bits (1639), Expect = 3.4e-181
Identity = 316/436 (72.48%), Postives = 370/436 (84.86%), Query Frame = 0

Query: 20  ALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILR 79
           ++L+ +YEIGKLLGHG+FAKVY ARNI + E VAIKVIDKEKI+K GL  HIKREISILR
Sbjct: 51  SILMDKYEIGKLLGHGSFAKVYLARNIHSGEDVAIKVIDKEKIVKSGLAGHIKREISILR 110

Query: 80  RVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFC 139
           RVRHP IV L EVMATK KIY VMEYVRGGEL+  V++GRL+E  AR+YFQQLIS+VAFC
Sbjct: 111 RVRHPYIVHLLEVMATKTKIYIVMEYVRGGELYNTVARGRLREGTARRYFQQLISSVAFC 170

Query: 140 HARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDGLFHTFCGTPAYVAPEVLAR 199
           H+RGVYHRDLK ENLLLD+ GN+KVSDFGLS VS+Q++Q+G+  TFCGTPAY+APEVL R
Sbjct: 171 HSRGVYHRDLKLENLLLDDKGNVKVSDFGLSVVSEQLKQEGICQTFCGTPAYLAPEVLTR 230

Query: 200 KGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLSR 259
           KGYE AK DIWSCGVILFVLMAGYLPF D+NI+ MY KIYKG+F+CP+WFSPEL RL++R
Sbjct: 231 KGYEGAKADIWSCGVILFVLMAGYLPFDDKNILVMYTKIYKGQFKCPKWFSPELARLVTR 290

Query: 260 LLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVEDDNDDVDSLS-----DQSQ 319
           +LDTNP+TR TIPEIM+++WFKKG+KH+KFYIE+DKLC  +DDNDD DS S       + 
Sbjct: 291 MLDTNPDTRITIPEIMKHRWFKKGFKHVKFYIENDKLCREDDDNDDDDSSSLSSGRSSTA 350

Query: 320 SESDSEIETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGEEARFVSGAPVSKIIS 379
           SE D+E + +R V S+PRPASLNAFDI+SFS   DLSGLFE+GG+ ARFVS AP++KIIS
Sbjct: 351 SEGDAEFDIKR-VDSMPRPASLNAFDILSFS---DLSGLFEEGGQGARFVSAAPMTKIIS 410

Query: 380 KLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPKLVMVEVKRKGGDKAE 439
           KLEEIAK V F VRKKD  V LEG REG KGPLTI  EIFELTP LV+VEVK+KGG+  E
Sbjct: 411 KLEEIAKEVKFMVRKKDWSVRLEGCREGAKGPLTIRVEIFELTPSLVVVEVKKKGGNIEE 470

Query: 440 YEQFCNKELKPALQKL 451
           YE+FCNKEL+P L+KL
Sbjct: 471 YEEFCNKELRPQLEKL 482

BLAST of Sgr019213 vs. ExPASy Swiss-Prot
Match: Q68Y49 (CBL-interacting protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK19 PE=1 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 6.5e-180
Identity = 314/478 (65.69%), Postives = 383/478 (80.13%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           LLLGRYE+G+LLGHGTFAKVY AR+  + E VAIKV+DKEK ++ GL+ HIKREI+ILRR
Sbjct: 32  LLLGRYELGRLLGHGTFAKVYQARSADSGEPVAIKVLDKEKAMRHGLVPHIKREIAILRR 91

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFCH 140
           VRHPNIV+LFEVMATK+KIYFVME VRGGELF +V+KGRLKE+ AR+YFQQL+SAV FCH
Sbjct: 92  VRHPNIVRLFEVMATKSKIYFVMELVRGGELFGRVAKGRLKEDTARRYFQQLVSAVGFCH 151

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDGLFHTFCGTPAYVAPEVLARK 200
           ARGV+HRDLKPENLL+DE+G+LKVSDFGLSAV+DQ   DGL HTFCGTP+YVAPEVLAR+
Sbjct: 152 ARGVFHRDLKPENLLVDEHGDLKVSDFGLSAVADQFHPDGLLHTFCGTPSYVAPEVLARR 211

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLSRL 260
           GY+ AK DIWSCG+ILFVLMAGYLPFHDQN+MAMY+KIY+GEFRCPRWFS +L  LL+R+
Sbjct: 212 GYDGAKADIWSCGIILFVLMAGYLPFHDQNLMAMYRKIYRGEFRCPRWFSKDLSSLLNRI 271

Query: 261 LDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVEDDNDDVDSL----------- 320
           LDTNPETR T+ E+ME++WF+KG++ ++FY+EDD++ S+ D ++D+  L           
Sbjct: 272 LDTNPETRITVKEVMESRWFQKGFRPVRFYVEDDQVHSLADGDNDMPELEPSEPPPPPPF 331

Query: 321 ----------SDQSQSESDSEIET-----------RRKVASLPRPASLNAFDIISFSPGF 380
                      ++S  ESDS + +           +R + SL RPASLNAFDIISFS GF
Sbjct: 332 PPPPPQQDDDGEESGWESDSSVASCPATLSSEERRQRPLGSLTRPASLNAFDIISFSKGF 391

Query: 381 DLSGLFEDGGEEARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLT 440
           DLSGLFE+ G E RF+S  P+  II+KLEEIAK+ SF VR+KD RVS+EG+REG+KGPLT
Sbjct: 392 DLSGLFEERGSEVRFISAEPMQTIITKLEEIAKVKSFFVRRKDWRVSIEGTREGLKGPLT 451

Query: 441 IAAEIFELTPKLVMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESAAPSQIPSDAE 467
           I AEIFELTP LV+VEVK+K GDK EY+ FCN+ELKP +Q L     + P+ IPSD E
Sbjct: 452 IGAEIFELTPSLVVVEVKKKAGDKEEYDDFCNRELKPGMQHLVHHMGSVPN-IPSDTE 508

BLAST of Sgr019213 vs. ExPASy TrEMBL
Match: A0A5A7UB69 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008270 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 3.9e-252
Identity = 444/467 (95.07%), Postives = 455/467 (97.43%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRP+PDKK E ++K+MPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMEN+WFKKGYKHIKFYIEDDK+CSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSQSESDSE-IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDNDDVDSLSDQSQSESDSE IETRRKV SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPK 420
           EARFVS APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAE+FELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESAAPSQIPSDAE 467
           LVMVEVKRKGGDKAEYEQFCN ELKPAL  LK+E+S  PS IPSD E
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467

BLAST of Sgr019213 vs. ExPASy TrEMBL
Match: A0A1S3AZF1 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103484445 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 3.9e-252
Identity = 444/467 (95.07%), Postives = 455/467 (97.43%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRP+PDKK E ++K+MPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMEN+WFKKGYKHIKFYIEDDK+CSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSQSESDSE-IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDNDDVDSLSDQSQSESDSE IETRRKV SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPK 420
           EARFVS APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAE+FELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESAAPSQIPSDAE 467
           LVMVEVKRKGGDKAEYEQFCN ELKPAL  LK+E+S  PS IPSD E
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467

BLAST of Sgr019213 vs. ExPASy TrEMBL
Match: A0A0A0KLP8 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6G519640 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.1e-250
Identity = 440/467 (94.22%), Postives = 453/467 (97.00%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRP+PDKK E ++K+MPALLLGRYEIGKLLGHGTFAKVYHARNIKTN+SVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNDSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMEN+WFKKGYKHIKFYIEDDK+CS+E
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSIE 300

Query: 301 DDNDDVDSLSDQSQSESDSE-IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DD DDVDSLSDQSQSESDSE IETRRKV SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDTDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPK 420
           EARFVS APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAE+FELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESAAPSQIPSDAE 467
           LVMVEVKRKGGDK EYEQFCN ELKPAL  LK+E+S  PS IPSD E
Sbjct: 421 LVMVEVKRKGGDKEEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467

BLAST of Sgr019213 vs. ExPASy TrEMBL
Match: A0A6J1CMJ2 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111012502 PE=3 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.5e-248
Identity = 439/474 (92.62%), Postives = 453/474 (95.57%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRP+PDKK +++KK+MPALLLGRYEIGKLLGHGTFAKVYHARNIKT+ESVAIKVIDKE
Sbjct: 1   MADRPDPDKKPDSNKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEA KVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAVKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFSPEL+RLL RLLDTNPETR TIPEIMEN+WFKKGYKHIKFYIEDDK+CSV+
Sbjct: 241 GEFRCPRWFSPELVRLLYRLLDTNPETRITIPEIMENRWFKKGYKHIKFYIEDDKVCSVQ 300

Query: 301 DDNDDVDSLSDQSQSESDSEIETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGEE 360
           +DNDD+DSLSDQSQSESDSEIE RRK ASLPRPASLNAFDIISFSPGFDLSGLFEDGGEE
Sbjct: 301 EDNDDIDSLSDQSQSESDSEIEFRRKTASLPRPASLNAFDIISFSPGFDLSGLFEDGGEE 360

Query: 361 ARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPKL 420
           ARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGS EGVKGPLTIAAEIFELTPKL
Sbjct: 361 ARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSMEGVKGPLTIAAEIFELTPKL 420

Query: 421 VMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESA--------APSQIPSDAE 467
           VMVEVKRKGGDKAEYE FCN ELKPALQ LKMEE +        APS IPSD E
Sbjct: 421 VMVEVKRKGGDKAEYEHFCNVELKPALQNLKMEEDSAAAPAPAPAPSHIPSDTE 474

BLAST of Sgr019213 vs. ExPASy TrEMBL
Match: A0A6J1EFG7 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111433713 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.2e-242
Identity = 436/474 (91.98%), Postives = 453/474 (95.57%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRP+ DKK E +KK+MPALLLGRYEIGKLLGHGTFAKVYHARNIKT++SVAIKVIDKE
Sbjct: 1   MADRPDTDKKTETTKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDQSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAVAFCHARGV+HRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVFHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVL RKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLTRKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMEN+WFKKGYKHIKFYIEDDK+CSVE
Sbjct: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDV---DSLSDQSQSESDSE-IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFED 360
           DD +DV   DS SDQSQSESDSE IETRRKV++LPRPASLNAFDIISFSPGFDLSGLFED
Sbjct: 301 DDQNDVDIIDSSSDQSQSESDSEIIETRRKVSTLPRPASLNAFDIISFSPGFDLSGLFED 360

Query: 361 GGEEARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFEL 420
           GGEEARFVS APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAE+FEL
Sbjct: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420

Query: 421 TPKLVMVEVKRKGGDKAEYEQFCNKELKPALQKLKME---ESAAP-SQIPSDAE 467
           T KLVMVEVKRKGGDKAEYEQFCN+ELKPAL  LK+E    +AAP S IPSD E
Sbjct: 421 TQKLVMVEVKRKGGDKAEYEQFCNEELKPALLNLKVEVEDSAAAPSSHIPSDTE 474

BLAST of Sgr019213 vs. TAIR 10
Match: AT4G18700.1 (CBL-interacting protein kinase 12 )

HSP 1 Score: 752.7 bits (1942), Expect = 1.8e-217
Identity = 380/462 (82.25%), Postives = 413/462 (89.39%), Query Frame = 0

Query: 1   MADRPEPDKKNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MA++   +      +    AL+LGRYE+GKLLGHGTFAKVY ARN+KTNESVAIKVIDKE
Sbjct: 1   MAEKITRETSLPKERSSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           K+LKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELF KV+KGRL
Sbjct: 61  KVLKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDG
Sbjct: 121 KEEVARKYFQQLISAVTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGY+AAKVDIWSCGVILFVLMAGYLPFHD+N+MAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVE 300
           GEFRCPRWFS EL RLLS+LL+TNPE RFT PEIMEN WFKKG+KHIKFY+EDDKLC+V 
Sbjct: 241 GEFRCPRWFSTELTRLLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVV 300

Query: 301 DD----NDDVDSLSDQSQSESDSE-IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFE 360
           DD    +D V+S  D + SES+ E +E RR+V  LPRPASLNAFDIISFS GFDLSGLF+
Sbjct: 301 DDDELESDSVESDRDSAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFD 360

Query: 361 DGGEEARFVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFE 420
           D GE +RFVSGAPVSKIISKLEEIAK+VSFTVRKKDCRVSLEGSR+GVKGPLTIAAEIFE
Sbjct: 361 DDGEGSRFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFE 420

Query: 421 LTPKLVMVEVKRKGGDKAEYEQFCNKELKPALQKLKMEESAA 458
           LTP LV+VEVK+KGGDK EYE FCN ELKP LQ L  ++  A
Sbjct: 421 LTPSLVVVEVKKKGGDKTEYEDFCNNELKPKLQNLTADDVVA 462

BLAST of Sgr019213 vs. TAIR 10
Match: AT5G45810.1 (CBL-interacting protein kinase 19 )

HSP 1 Score: 701.0 bits (1808), Expect = 6.2e-202
Identity = 354/472 (75.00%), Postives = 410/472 (86.86%), Query Frame = 0

Query: 10  KNENSKKEMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIA 69
           K +  +    AL+LG+YE+G+LLGHGTFAKVY ARN ++ ESVAIKVIDKEK+LK GLIA
Sbjct: 12  KKKQDQSNHQALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVIDKEKVLKSGLIA 71

Query: 70  HIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYF 129
           HIKREISILRRVRHPNIVQLFEVMATK+KIYFVMEYV+GGELF KV+KGRLKEE+ARKYF
Sbjct: 72  HIKREISILRRVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKYF 131

Query: 130 QQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDGLFHTFCGTP 189
           QQLISAV+FCH RGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQI+QDGLFHTFCGTP
Sbjct: 132 QQLISAVSFCHFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP 191

Query: 190 AYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWF 249
           AYVAPEVLARKGY+ AKVDIWSCGVILFVLMAG+LPFHD+N+MAMYKKIY+G+FRCPRWF
Sbjct: 192 AYVAPEVLARKGYDGAKVDIWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWF 251

Query: 250 SPELIRLLSRLLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDD-KLCSVEDDN--DDV 309
             E+ RLL R+L+T PE RFT+P+IME  WFKKG+KHIKFY+EDD +LC+V DD+  + +
Sbjct: 252 PVEINRLLIRMLETKPERRFTMPDIMETSWFKKGFKHIKFYVEDDHQLCNVADDDEIESI 311

Query: 310 DSLSDQSQSESDSE----IETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGEEAR 369
           +S+S +S + S+ E     + RR+  S+PRPASLNAFD+ISFSPGFDLSGLFED GE +R
Sbjct: 312 ESVSGRSSTVSEPEDFESFDGRRRGGSMPRPASLNAFDLISFSPGFDLSGLFEDDGEGSR 371

Query: 370 FVSGAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREG-VKGPLTIAAEIFELTPKLV 429
           FVSGAPV +IISKLEEIA++VSFTVRKKDC+VSLEGSREG +KGPL+IAAEIFELTP LV
Sbjct: 372 FVSGAPVGQIISKLEEIARIVSFTVRKKDCKVSLEGSREGSMKGPLSIAAEIFELTPALV 431

Query: 430 MVEVKRKGGDKAEYEQFCNKELKPALQKLKMEES-------AAPSQIPSDAE 467
           +VEVK+KGGDK EY++FCNKELKP LQ L  E         + PS + SD +
Sbjct: 432 VVEVKKKGGDKMEYDEFCNKELKPKLQNLSSENGQRVSGSRSLPSFLLSDTD 483

BLAST of Sgr019213 vs. TAIR 10
Match: AT1G29230.1 (CBL-interacting protein kinase 18 )

HSP 1 Score: 682.2 bits (1759), Expect = 3.0e-196
Identity = 339/452 (75.00%), Postives = 394/452 (87.17%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           +L+G+YE+GKLLGHGTFAKVY A+NIK+ + VAIKVIDKEKI+K GL+AHIKREISILRR
Sbjct: 69  ILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKIMKSGLVAHIKREISILRR 128

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFCH 140
           VRHP IV LFEVMATK+KIYFVMEYV GGELF  V+KGRL EE AR+YFQQLIS+V+FCH
Sbjct: 129 VRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPEETARRYFQQLISSVSFCH 188

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDGLFHTFCGTPAYVAPEVLARK 200
            RGVYHRDLKPENLLLD  GNLKVSDFGLSAV++Q++QDGL HTFCGTPAY+APEVL RK
Sbjct: 189 GRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLCHTFCGTPAYIAPEVLTRK 248

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLSRL 260
           GY+AAK D+WSCGVILFVLMAG++PF+D+NIM MYKKIYKGEFRCPRWFS +L+RLL+RL
Sbjct: 249 GYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGEFRCPRWFSSDLVRLLTRL 308

Query: 261 LDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVEDDNDDVDSLSDQSQ--SESD 320
           LDTNP+TR TIPEIM+N+WFKKG+KH+KFYIEDDKLC  ++D ++  S S +S   SESD
Sbjct: 309 LDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDEDEEEEASSSGRSSTVSESD 368

Query: 321 SEIETRRK-VASLPRPASLNAFDIISFSPGFDLSGLF-EDGGEEARFVSGAPVSKIISKL 380
           +E + +R  + S+PRP+SLNAFDIISFS GFDLSGLF E+GGE  RFVSGAPVSKIISKL
Sbjct: 369 AEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEEEGGEGTRFVSGAPVSKIISKL 428

Query: 381 EEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPKLVMVEVKRKGGDKAEYE 440
           EEIAK+VSFTVRKK+  + LEG REG KGPLTIAAEIFELTP LV+VEVK+KGGD+ EYE
Sbjct: 429 EEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFELTPSLVVVEVKKKGGDREEYE 488

Query: 441 QFCNKELKPALQKLKMEESAAPS--QIPSDAE 467
           +FCNKEL+P L+KL  EE        +PSD E
Sbjct: 489 EFCNKELRPELEKLIHEEVVVEEALYLPSDTE 520

BLAST of Sgr019213 vs. TAIR 10
Match: AT2G34180.1 (CBL-interacting protein kinase 13 )

HSP 1 Score: 636.0 bits (1639), Expect = 2.4e-182
Identity = 316/436 (72.48%), Postives = 370/436 (84.86%), Query Frame = 0

Query: 20  ALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILR 79
           ++L+ +YEIGKLLGHG+FAKVY ARNI + E VAIKVIDKEKI+K GL  HIKREISILR
Sbjct: 51  SILMDKYEIGKLLGHGSFAKVYLARNIHSGEDVAIKVIDKEKIVKSGLAGHIKREISILR 110

Query: 80  RVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFC 139
           RVRHP IV L EVMATK KIY VMEYVRGGEL+  V++GRL+E  AR+YFQQLIS+VAFC
Sbjct: 111 RVRHPYIVHLLEVMATKTKIYIVMEYVRGGELYNTVARGRLREGTARRYFQQLISSVAFC 170

Query: 140 HARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDGLFHTFCGTPAYVAPEVLAR 199
           H+RGVYHRDLK ENLLLD+ GN+KVSDFGLS VS+Q++Q+G+  TFCGTPAY+APEVL R
Sbjct: 171 HSRGVYHRDLKLENLLLDDKGNVKVSDFGLSVVSEQLKQEGICQTFCGTPAYLAPEVLTR 230

Query: 200 KGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLSR 259
           KGYE AK DIWSCGVILFVLMAGYLPF D+NI+ MY KIYKG+F+CP+WFSPEL RL++R
Sbjct: 231 KGYEGAKADIWSCGVILFVLMAGYLPFDDKNILVMYTKIYKGQFKCPKWFSPELARLVTR 290

Query: 260 LLDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVEDDNDDVDSLS-----DQSQ 319
           +LDTNP+TR TIPEIM+++WFKKG+KH+KFYIE+DKLC  +DDNDD DS S       + 
Sbjct: 291 MLDTNPDTRITIPEIMKHRWFKKGFKHVKFYIENDKLCREDDDNDDDDSSSLSSGRSSTA 350

Query: 320 SESDSEIETRRKVASLPRPASLNAFDIISFSPGFDLSGLFEDGGEEARFVSGAPVSKIIS 379
           SE D+E + +R V S+PRPASLNAFDI+SFS   DLSGLFE+GG+ ARFVS AP++KIIS
Sbjct: 351 SEGDAEFDIKR-VDSMPRPASLNAFDILSFS---DLSGLFEEGGQGARFVSAAPMTKIIS 410

Query: 380 KLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPKLVMVEVKRKGGDKAE 439
           KLEEIAK V F VRKKD  V LEG REG KGPLTI  EIFELTP LV+VEVK+KGG+  E
Sbjct: 411 KLEEIAKEVKFMVRKKDWSVRLEGCREGAKGPLTIRVEIFELTPSLVVVEVKKKGGNIEE 470

Query: 440 YEQFCNKELKPALQKL 451
           YE+FCNKEL+P L+KL
Sbjct: 471 YEEFCNKELRPQLEKL 482

BLAST of Sgr019213 vs. TAIR 10
Match: AT5G45820.1 (CBL-interacting protein kinase 20 )

HSP 1 Score: 501.9 bits (1291), Expect = 5.5e-142
Identity = 254/433 (58.66%), Postives = 321/433 (74.13%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           +L+ +YE+G+LLG GTFAKVYHARNIKT ESVAIKVIDK+K+ K GLI  IKREIS++R 
Sbjct: 7   VLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRL 66

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFCH 140
           VRHP++V L EVMA+K KIYF MEYV+GGELF KVSKG+LKE +ARKYFQQLI A+ +CH
Sbjct: 67  VRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVSKGKLKENIARKYFQQLIGAIDYCH 126

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIQQDGLFHTFCGTPAYVAPEVLARK 200
           +RGVYHRDLKPENLLLDENG+LK+SDFGLSA+ +  QQDGL HT CGTPAYVAPEV+ +K
Sbjct: 127 SRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGKK 186

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLSRL 260
           GY+ AK D+WSCGV+L+VL+AG+LPFH+QN++ MY+KI KGEF+CP WF PE+ +LLSR+
Sbjct: 187 GYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSRI 246

Query: 261 LDTNPETRFTIPEIMENKWFKKGYKHIKFYIEDDKLCSVEDDNDDVDSLSDQSQSESDSE 320
           LD NP +R  I +IMEN WF+KG+K I+             ++  +DSL         S+
Sbjct: 247 LDPNPNSRIKIEKIMENSWFQKGFKKIE--------TPKSPESHQIDSLI--------SD 306

Query: 321 IETRRKVASLPRPASLNAFDII-SFSPGFDLSGLFE-DGGEEARFVSGAPVSKIISKLEE 380
           +     V    +P S NAFD+I S S GFDLSGLFE +   E++F +     +I+SK EE
Sbjct: 307 VHAAFSV----KPMSYNAFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIVSKFEE 366

Query: 381 IA-KLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEIFELTPKLVMVEVKRKGGDKAEYEQ 440
           IA     F + K D  V +E  REG KG L I  EIFE+T    MVE K+ GGD  EY+Q
Sbjct: 367 IATSSERFNLTKSDVGVKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQ 419

Query: 441 FCNKELKPALQKL 451
           FC++EL+P+L+ +
Sbjct: 427 FCDRELRPSLKDI 419

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882513.14.7e-25295.07CBL-interacting serine/threonine-protein kinase 12-like [Benincasa hispida][more]
XP_008439731.18.0e-25295.07PREDICTED: CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo... [more]
XP_011658253.14.4e-25094.22CBL-interacting serine/threonine-protein kinase 12 [Cucumis sativus] >KGN49312.1... [more]
XP_022142362.13.1e-24892.62CBL-interacting serine/threonine-protein kinase 12-like [Momordica charantia][more]
XP_022926641.12.6e-24291.98CBL-interacting serine/threonine-protein kinase 12-like [Cucurbita moschata] >KA... [more]
Match NameE-valueIdentityDescription
Q9SN432.5e-21682.25CBL-interacting serine/threonine-protein kinase 12 OS=Arabidopsis thaliana OX=37... [more]
Q9FJ558.7e-20175.00CBL-interacting serine/threonine-protein kinase 19 OS=Arabidopsis thaliana OX=37... [more]
Q9LP514.2e-19575.00CBL-interacting serine/threonine-protein kinase 18 OS=Arabidopsis thaliana OX=37... [more]
O229713.4e-18172.48CBL-interacting serine/threonine-protein kinase 13 OS=Arabidopsis thaliana OX=37... [more]
Q68Y496.5e-18065.69CBL-interacting protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Match NameE-valueIdentityDescription
A0A5A7UB693.9e-25295.07Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3AZF13.9e-25295.07Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0KLP82.1e-25094.22Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6... [more]
A0A6J1CMJ21.5e-24892.62Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1EFG71.2e-24291.98Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT4G18700.11.8e-21782.25CBL-interacting protein kinase 12 [more]
AT5G45810.16.2e-20275.00CBL-interacting protein kinase 19 [more]
AT1G29230.13.0e-19675.00CBL-interacting protein kinase 18 [more]
AT2G34180.12.4e-18272.48CBL-interacting protein kinase 13 [more]
AT5G45820.15.5e-14258.66CBL-interacting protein kinase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 26..280
e-value: 3.3E-110
score: 382.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 26..280
e-value: 1.2E-76
score: 257.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 26..280
score: 52.625843
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 17..334
e-value: 3.1E-107
score: 360.6
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 335..451
e-value: 1.5E-40
score: 139.9
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 28..294
e-value: 1.2E-12
score: 45.3
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 30..232
e-value: 1.6E-14
score: 51.8
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 28..294
e-value: 1.2E-12
score: 45.3
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 26..239
e-value: 9.7E-37
score: 123.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 302..326
NoneNo IPR availablePANTHERPTHR43895:SF96NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 18..461
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 18..461
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 337..448
e-value: 1.82936E-49
score: 162.742
NoneNo IPR availableCDDcd14663STKc_SnRK3coord: 25..279
e-value: 7.82694E-170
score: 475.741
IPR004041NAF domainPFAMPF03822NAFcoord: 333..389
e-value: 9.1E-24
score: 83.4
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 144..156
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 32..55
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 331..355
score: 14.624336
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 22..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr019213.1Sgr019213.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity