Sgr018742 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr018742
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionprotein NRT1/ PTR FAMILY 5.4
Locationtig00153210: 177928 .. 181956 (+)
RNA-Seq ExpressionSgr018742
SyntenySgr018742
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACTAAAGAGAAAGCTCCGACCCCCATCAACGTCGGCAAGAAGTTTTCCGATGATCGGAACGTCTCCGACCGGCATAATAAGCTGCCTCCCGGAGGTGGCTGGAAAGCTGCTATCTTCGTCATCTGTAAAACTCCCAACTTCTCCTTTTTGATCTTTTTGGTTTTGAATTGATTTTGTGTGGTGTGGTGTGGTTAATTACTGTCGATTGGATTCATCGATGGCAGCCGTGGAGATCGCCGAGCAATTCGCTTTCATCGGCTTGTCTAGTAATCTCATCACGTACTTCACCACCGTCCTCCACGAACCCACCGCCATGGCCGCCAAACATGTCAACAACTGGGTCGGCGTCTCCGCCGTCTTCCCTTTACTCGGCGCCTTCGTCGCCGACTCATTGCTAGGCCGTTTCAGAACCATCATCTTCTCCTCCGTCATATATTTTCTGGTGCGTGCCGTGCCATACACACACACACACACACATATATATATATACATATATATTTGTTATGTTAAATACATATCATGGGTTTCTTTTTTTACAGTGGTTTTTGGATGGCGTTGCAGGGAATGGTGTTGTTGACTTTATCGGTGTCTGTGATCGGGGAGAGTCACCGGAAGGCGGCGTTTTTTGTGGCGCTGTACATTCTGTCGGTGGGGGAAGGGGGACACCGGCCGTGCGTTCAGACGTTCGCCGCCGATCAGTTCGATGAGGGGTCGCCGGAGGAGAGGAAGAAGAAAAGCTCGTTCTTCAATTGGTGGTACGTGGGCCTGGTGGTTGGCTCCACCCTTGCTGTCTTTGTGGTTATTTACGTTCAGGTATTGCTATTGCTTTCTCTAATGTTTAATCTAACTGTAATTATAAAAAAAAAAATTAATTAATTAATAATCTTTCATTTGAAAAATAGTTATGTTTTTTTTTATATTCTAAATTAAAATTATTTGAATATCGAAAGCTTAAAACACATTTTTAGTCTCTATATATAATTTGACTCTAGTAACATTTTAGTTTTTATACTTCTTTTTGTAATGATATACTTTTAAAATATAACGATAGAGTTCTTGACATCAATTTTTTTTTCCATATTTTTGCAACTATATTTTTTTATACTAATTTGCTTTCCTATAAATGTTTTAGAATAAGGGATCATCCAAAAAAAACAAAGAAAAAAAAAAAGAGTTACATAAAGGGACAACTTAAACTATATATTTATGGTTTTAGTTTTTATTTTTTTAGAATATAACATAACATATTTAGTGGGAAGGATCTAAATCTTCTACCTATAAACAAGATTACTAATATCTTAATTATTGAATTATGCTCAAATTGGCAATCTTAAACTTTTTAAATTTTGAATTTTAGTATGTATTTATATTTAGTATCTCTATCTAATTGTATATATATAACATGTGTATAATAAATAATGTGAAACCATGTTGCAAATAACTTCGAAACATAATTCTTAATGGTAAAGAGTAGATAAAAAGTTCATGGATCAAAATCAAGGTTTCAACCGTGTAGAAACTAAATTAAAATAAATAAATAAAAATTTAGAGACCAAAATTATGTTTAAATCGACTTAACATACTTTTTTTTATGATAGTACAAATAAATAAGAGAAAATTCAAGCTTTAAATCAGTTAGAATTTTTTGCTAACACGAACATAGCTTAATGGTTAAGATATTTCTACTTTCTCTAAGAGGTCGCAGGTTTGAATCCCCCAGTCCCCACTTTATGATATTCTCAACGAAAAAAAAATCAACTAGACTTTTTGATAATAATTCAATATCACTCAATTACATTTTTTATGGGAATAAAACAAATAGCATATTACATTTATAGGAACTCATTTTTAACTTTTTTAAAAAAAAAACTCATTTTTAAATTTATAAAAATAACAAATGAAAAAAAAAACACCTCACATGTCATGTTTAATATTTTAAAATATATATTATTTTTGAAAAAAAATGCAAATGGGCTGTTATATTTTGAAATATATTATTTTATTAGGGCATTAACAAAAAGTCCAACACTGCCAAATCGAGTATAACTAAACTAATTAAGGCACTTAGTAACCTTTTAAAATTCAGAGGTTCGAATCTCCACCCTATTATTGAACTAAAAAAACAAAAAGTTCAGACACTTTTTTTTTTTATATTATATTATCTTCCACTTTTAATTTCTTTCATTATATATAAATATATAATTATCTTTCATGCATTTATTTATATAAATCAGCTTTTTTTTCTTGTTTTTTTTTTGAAACAAATAGGATAATATCAGCTGGGCCGTGAGTTTTGGAGTTCTGGCCGGAGTGTTGGGGGCGGCGATAGTTTTGTTTCTGGGCGGAGTGAAGAAGTACAGACGGCAAGGTCCCCTCGGAAGCCCGTTGACGAGGATAGCTCAGGTGGTGGTGGCGGCGGCGAGGAAGTGGCGCGTGGACGAGACGCGATATGGATGGAGAGTGTGCTATGAAGACACCGAGGGTGAACACATGGTTACGACTTTGGGTCGCACAAACCAATTCAGGTCACCAAAATCTGCAGAGATTCCGTAAATAATAATAATGATAATATCTGTAAATGAGTAATCTGATGTGTTGTTGTCCGGCGGGCATGCAGGGTATTGGACAAAGCGACGTACATAGACGAAGTGGACGAAGGGAGGAAGCGGCGGAATCCGTGGCGGCTGAGGACGGTGAGTGAGGTGGAGGAAGTGAAGCTGGTGGGGCGGCTGATTCCGGTGTGGGTGAGCTGCCTGATGTTCGCGGTGGTGCAAGCTCAGCTCCACACCTACTTCACCAAGCAAGGCAGCACCATGCTCCGCTCCGTCGGCCCCCGCTTCCACCTCCCTCCGGCCTCCCTCCAGGGCGTCGTCGGCCTCACCATCCTCCTCACCATTCTCTTCTACGACCGCGTGTTTGTCCCCGCCGCCAGAAACTTTACACGCCACCACTCAGGTACCTAGTACGCCCTTTCCTACCAACTCCACCTTTTCAAAATATTACTATATACACTCAGCTAACACCGATTATTCAATGCAAAGACAATTTGTGATATTTCACTGAGATAAGAATTAAGAAAAAATACTTCTATATTGACTCGACAACATGAATTTATGATTGGCCACCAATAAGTTTTTAACAGGTGGTTATTTTTTTAAAAATAAAAAAATTTCTCCTCTCTACGTTCCGTCTCGATCTCGGTGGGCAGCACGAAATTCTGTTGCCTCAACAAAAAAATATTGTTCTTTACTAAATCAAATTATAAATACTAAATTGAAAATTTTAAAATTATAGAGATCAAATTGAAACTATGTCCAAACTATCATATTCTTAATTAAACAAAGAAAAAAAATTAAACCAATATTTAATTTCAAAGACTATAAAACAAAAAAATTGATACCATGCATATCCATGAACAAAGGCATAACGGTGCTGCAAAGAATAGGGATAGGCCTCTTCATCTCCATCCTCAACATGTCGGTGTCCGCTCTGGTAGAGGCCAAGAGGGTGGCCGTCGCCGCCGACCACCACCTAACCGACACACCAAAAGCGGTGGTTCCGATGACAATCTGGTGGCTGATTCCACAGTACATGCTCTGCGGCGTCTCCGACGCCTTCACCATCGTCGGACTCCAGGAGCTTTTCTACGACCAAATGCCCGAATCCATGAGGAGCTTGGGAGCCGCAGCCTACATCAGCATCGTCGGAGTTGGAAACTTCCTCAGCAGCGCCATCATATCCGTCGTCCAGGCCGTCACCGGCGCCGGGAGTGGCCGGAAATGGCTGGACGACAATCTGAACCGCGCACAGCTGCATTACTTTTACTGGGTTTTGGCGGCTTTGAGTGCTCTGAATCTGTGCGCCTACGTTTGGATCGCCAATGGGTTTGTGTACAAAAGAGTTGAAGGAAATATTAATGGTGATGATCGGATGAGTGTGGAGAAGGAGCTGAGCTTGAAGAACAGTAACAATGGCCGCTGTGGAGATGATTTGATTAATGAGAGAGATAGAGAGAATCCATAA

mRNA sequence

ATGGAGACTAAAGAGAAAGCTCCGACCCCCATCAACGTCGGCAAGAAGTTTTCCGATGATCGGAACGTCTCCGACCGGCATAATAAGCTGCCTCCCGGAGGTGGCTGGAAAGCTGCTATCTTCGTCATCTCCGTGGAGATCGCCGAGCAATTCGCTTTCATCGGCTTGTCTAGTAATCTCATCACGTACTTCACCACCGTCCTCCACGAACCCACCGCCATGGCCGCCAAACATGTCAACAACTGGGTCGGCGTCTCCGCCGTCTTCCCTTTACTCGGCGCCTTCGTCGCCGACTCATTGCTAGGCCGTTTCAGAACCATCATCTTCTCCTCCGTCATATATTTTCTGGGAATGGTGTTGTTGACTTTATCGGTGTCTGTGATCGGGGAGAGTCACCGGAAGGCGGCGTTTTTTGTGGCGCTGTACATTCTGTCGGTGGGGGAAGGGGGACACCGGCCGTGCGTTCAGACGTTCGCCGCCGATCAGTTCGATGAGGGGTCGCCGGAGGAGAGGAAGAAGAAAAGCTCGTTCTTCAATTGGTGGTACGTGGGCCTGGTGGTTGGCTCCACCCTTGCTGTCTTTGTGGTTATTTACGTTCAGGATAATATCAGCTGGGCCGTGAGTTTTGGAGTTCTGGCCGGAGTGTTGGGGGCGGCGATAGTTTTGTTTCTGGGCGGAGTGAAGAAGTACAGACGGCAAGGTCCCCTCGGAAGCCCGTTGACGAGGATAGCTCAGGTGGTGGTGGCGGCGGCGAGGAAGTGGCGCGTGGACGAGACGCGATATGGATGGAGAGTGTGCTATGAAGACACCGAGGGTGAACACATGGTTACGACTTTGGGTCGCACAAACCAATTCAGGGTATTGGACAAAGCGACGTACATAGACGAAGTGGACGAAGGGAGGAAGCGGCGGAATCCGTGGCGGCTGAGGACGGTGAGTGAGGTGGAGGAAGTGAAGCTGGTGGGGCGGCTGATTCCGGTGTGGGTGAGCTGCCTGATGTTCGCGGTGGTGCAAGCTCAGCTCCACACCTACTTCACCAAGCAAGGCAGCACCATGCTCCGCTCCGTCGGCCCCCGCTTCCACCTCCCTCCGGCCTCCCTCCAGGGCGTCGTCGGCCTCACCATCCTCCTCACCATTCTCTTCTACGACCGCGTGTTTGTCCCCGCCGCCAGAAACTTTACACGCCACCACTCAGGCATAACGGTGCTGCAAAGAATAGGGATAGGCCTCTTCATCTCCATCCTCAACATGTCGGTGTCCGCTCTGGTAGAGGCCAAGAGGGTGGCCGTCGCCGCCGACCACCACCTAACCGACACACCAAAAGCGGTGGTTCCGATGACAATCTGGTGGCTGATTCCACAGTACATGCTCTGCGGCGTCTCCGACGCCTTCACCATCGTCGGACTCCAGGAGCTTTTCTACGACCAAATGCCCGAATCCATGAGGAGCTTGGGAGCCGCAGCCTACATCAGCATCGTCGGAGTTGGAAACTTCCTCAGCAGCGCCATCATATCCGTCGTCCAGGCCGTCACCGGCGCCGGGAGTGGCCGGAAATGGCTGGACGACAATCTGAACCGCGCACAGCTGCATTACTTTTACTGGGTTTTGGCGGCTTTGAGTGCTCTGAATCTGTGCGCCTACGTTTGGATCGCCAATGGGTTTGTGTACAAAAGAGTTGAAGGAAATATTAATGGTGATGATCGGATGAGTGTGGAGAAGGAGCTGAGCTTGAAGAACAGTAACAATGGCCGCTGTGGAGATGATTTGATTAATGAGAGAGATAGAGAGAATCCATAA

Coding sequence (CDS)

ATGGAGACTAAAGAGAAAGCTCCGACCCCCATCAACGTCGGCAAGAAGTTTTCCGATGATCGGAACGTCTCCGACCGGCATAATAAGCTGCCTCCCGGAGGTGGCTGGAAAGCTGCTATCTTCGTCATCTCCGTGGAGATCGCCGAGCAATTCGCTTTCATCGGCTTGTCTAGTAATCTCATCACGTACTTCACCACCGTCCTCCACGAACCCACCGCCATGGCCGCCAAACATGTCAACAACTGGGTCGGCGTCTCCGCCGTCTTCCCTTTACTCGGCGCCTTCGTCGCCGACTCATTGCTAGGCCGTTTCAGAACCATCATCTTCTCCTCCGTCATATATTTTCTGGGAATGGTGTTGTTGACTTTATCGGTGTCTGTGATCGGGGAGAGTCACCGGAAGGCGGCGTTTTTTGTGGCGCTGTACATTCTGTCGGTGGGGGAAGGGGGACACCGGCCGTGCGTTCAGACGTTCGCCGCCGATCAGTTCGATGAGGGGTCGCCGGAGGAGAGGAAGAAGAAAAGCTCGTTCTTCAATTGGTGGTACGTGGGCCTGGTGGTTGGCTCCACCCTTGCTGTCTTTGTGGTTATTTACGTTCAGGATAATATCAGCTGGGCCGTGAGTTTTGGAGTTCTGGCCGGAGTGTTGGGGGCGGCGATAGTTTTGTTTCTGGGCGGAGTGAAGAAGTACAGACGGCAAGGTCCCCTCGGAAGCCCGTTGACGAGGATAGCTCAGGTGGTGGTGGCGGCGGCGAGGAAGTGGCGCGTGGACGAGACGCGATATGGATGGAGAGTGTGCTATGAAGACACCGAGGGTGAACACATGGTTACGACTTTGGGTCGCACAAACCAATTCAGGGTATTGGACAAAGCGACGTACATAGACGAAGTGGACGAAGGGAGGAAGCGGCGGAATCCGTGGCGGCTGAGGACGGTGAGTGAGGTGGAGGAAGTGAAGCTGGTGGGGCGGCTGATTCCGGTGTGGGTGAGCTGCCTGATGTTCGCGGTGGTGCAAGCTCAGCTCCACACCTACTTCACCAAGCAAGGCAGCACCATGCTCCGCTCCGTCGGCCCCCGCTTCCACCTCCCTCCGGCCTCCCTCCAGGGCGTCGTCGGCCTCACCATCCTCCTCACCATTCTCTTCTACGACCGCGTGTTTGTCCCCGCCGCCAGAAACTTTACACGCCACCACTCAGGCATAACGGTGCTGCAAAGAATAGGGATAGGCCTCTTCATCTCCATCCTCAACATGTCGGTGTCCGCTCTGGTAGAGGCCAAGAGGGTGGCCGTCGCCGCCGACCACCACCTAACCGACACACCAAAAGCGGTGGTTCCGATGACAATCTGGTGGCTGATTCCACAGTACATGCTCTGCGGCGTCTCCGACGCCTTCACCATCGTCGGACTCCAGGAGCTTTTCTACGACCAAATGCCCGAATCCATGAGGAGCTTGGGAGCCGCAGCCTACATCAGCATCGTCGGAGTTGGAAACTTCCTCAGCAGCGCCATCATATCCGTCGTCCAGGCCGTCACCGGCGCCGGGAGTGGCCGGAAATGGCTGGACGACAATCTGAACCGCGCACAGCTGCATTACTTTTACTGGGTTTTGGCGGCTTTGAGTGCTCTGAATCTGTGCGCCTACGTTTGGATCGCCAATGGGTTTGTGTACAAAAGAGTTGAAGGAAATATTAATGGTGATGATCGGATGAGTGTGGAGAAGGAGCTGAGCTTGAAGAACAGTAACAATGGCCGCTGTGGAGATGATTTGATTAATGAGAGAGATAGAGAGAATCCATAA

Protein sequence

METKEKAPTPINVGKKFSDDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVIGESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSPLTRIAQVVVAAARKWRVDETRYGWRVCYEDTEGEHMVTTLGRTNQFRVLDKATYIDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNLCAYVWIANGFVYKRVEGNINGDDRMSVEKELSLKNSNNGRCGDDLINERDRENP
Homology
BLAST of Sgr018742 vs. NCBI nr
Match: XP_022138943.1 (protein NRT1/ PTR FAMILY 5.4 [Momordica charantia])

HSP 1 Score: 929.1 bits (2400), Expect = 1.9e-266
Identity = 474/584 (81.16%), Postives = 520/584 (89.04%), Query Frame = 0

Query: 16  KFSDDR-NVSDRHNKLP--PGGGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPT 75
           KFS DR N+SDRHN+ P  PGGGWKAAIFVI VEIAEQFAFIGLSSNLI YFTTVL EPT
Sbjct: 5   KFSGDRNNLSDRHNRPPPAPGGGWKAAIFVICVEIAEQFAFIGLSSNLIMYFTTVLGEPT 64

Query: 76  AMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVIGESH 135
           A AAK VNNW GVSAVFP+LGAFVADS LGRF+TII S++IYFLGMVLL++SVS +G  H
Sbjct: 65  AAAAKQVNNWAGVSAVFPILGAFVADSFLGRFKTIIISTLIYFLGMVLLSVSVSWVGSGH 124

Query: 136 RKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGSTLA 195
           RKA   VALYI+SVGEGGHRPCVQTFAADQFDEGSPEER+KKSSFFNWW+VGLVVGSTLA
Sbjct: 125 RKAVVLVALYIMSVGEGGHRPCVQTFAADQFDEGSPEERRKKSSFFNWWFVGLVVGSTLA 184

Query: 196 VFVVIYVQDNIS--WAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSPLTRIAQVVVAA 255
           VF+VIYVQDN++  W +SFGVLAGVL AAIVLFL GVKKYR+QGPLGSPLT IAQVVVAA
Sbjct: 185 VFLVIYVQDNMATGWGLSFGVLAGVLAAAIVLFLAGVKKYRQQGPLGSPLTTIAQVVVAA 244

Query: 256 ARKWRVDETRYGWRVCYEDT----EGEHMVTTLGRTNQFRVLDKATYIDEVDEGRKRRNP 315
           ARKWRVDETR+GWRVCYED     EG H++TTLG+TNQFR+L+KAT ++E DE RK R+ 
Sbjct: 245 ARKWRVDETRHGWRVCYEDNDAHHEGRHVLTTLGQTNQFRILNKATLMEEADEKRKVRDR 304

Query: 316 WRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPPAS 375
           WR+ TV EVEEVKLV RL+PVWVSCLMF VVQAQLHT+FTKQGSTMLRS+GP F LPPAS
Sbjct: 305 WRVSTVGEVEEVKLVVRLVPVWVSCLMFGVVQAQLHTFFTKQGSTMLRSIGPHFQLPPAS 364

Query: 376 LQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVEAK 435
           LQGVVGLTILLT+LFYDRVFVPAARNFT HHSGITVLQRIGIGLFISILNM+VSALVE K
Sbjct: 365 LQGVVGLTILLTVLFYDRVFVPAARNFTAHHSGITVLQRIGIGLFISILNMAVSALVETK 424

Query: 436 RVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSLGA 495
           RVAVAA+H LTD PKAVVPMTIWWLIPQYMLC +SDAF IVGLQELFYDQMPESMRSLGA
Sbjct: 425 RVAVAANHGLTDIPKAVVPMTIWWLIPQYMLCXISDAFAIVGLQELFYDQMPESMRSLGA 484

Query: 496 AAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNLCA 555
           AA ISI+GVGNFLSSA+ISVVQA T A  GRKWL+DNLNR+ LHYFYW+LAALSALNLC 
Sbjct: 485 AANISIIGVGNFLSSAVISVVQAATVARGGRKWLEDNLNRSNLHYFYWILAALSALNLCG 544

Query: 556 YVWIANGFVYKRVEGNINGDDRMSVEKELSLKNSNNGRCGDDLI 591
           YVW+ANGFVYKRV+  + G++   VEKE+S KNSNNG  GDD+I
Sbjct: 545 YVWMANGFVYKRVDQEL-GNNINGVEKEVSFKNSNNGCHGDDMI 587

BLAST of Sgr018742 vs. NCBI nr
Match: XP_038892997.1 (protein NRT1/ PTR FAMILY 5.4 [Benincasa hispida])

HSP 1 Score: 905.2 bits (2338), Expect = 3.0e-259
Identity = 466/600 (77.67%), Postives = 506/600 (84.33%), Query Frame = 0

Query: 1   METKEKAPTP-INVGKKFSDDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSN 60
           ME  +    P IN+  KF D    S+R   +PPGGGWKAAIF+I VE+AEQFAFIGLSSN
Sbjct: 1   MEKHKSLNNPVINLSNKFLDRPTASNRPT-MPPGGGWKAAIFIIFVEVAEQFAFIGLSSN 60

Query: 61  LITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMV 120
           LI YFTTV HEP  +AAK VNNWVGVSAVFPLLGAFVADSLLGRF+TII +S+IY LGMV
Sbjct: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIYCLGMV 120

Query: 121 LLTLSVSVIGESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFN 180
           LLT+S +VIG  HRK  FF+ALYILSVGEGGHRPCVQTFAADQFDE SPEERKKKSSFFN
Sbjct: 121 LLTISATVIGADHRKPVFFLALYILSVGEGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180

Query: 181 WWYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSP 240
           WWYVGLVVGST AVFVVIYVQDNI W +SFG+LAGVL AAI+LFL GVKKYRRQ P+GSP
Sbjct: 181 WWYVGLVVGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRYGWRVCYEDT-------EGEHMVTTLGRTNQFRVLDKAT 300
           LTRIAQVVVAA RKWRVDETR+ WRVCY++        E ++ + TL RTNQFR+LDKA 
Sbjct: 241 LTRIAQVVVAAVRKWRVDETRHEWRVCYDEENHAKNEGESQYKLMTLVRTNQFRILDKAA 300

Query: 301 YIDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTM 360
            IDE DE RK+RNPWRL TV EVEEVKLV RLIPVWVSCLMFAVVQAQ+HT+FTKQGSTM
Sbjct: 301 LIDEEDEARKKRNPWRLSTVREVEEVKLVVRLIPVWVSCLMFAVVQAQMHTFFTKQGSTM 360

Query: 361 LRSVGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFI 420
           LRSVGP F LPPASLQGVVGLTILLT+LFYDRVFVPAARNFT HHSGITVLQRIG+GLFI
Sbjct: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTAHHSGITVLQRIGMGLFI 420

Query: 421 SILNMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQEL 480
           SIL M VSALVEAKRV +AA H LTDTPK  +PMTIWWLIPQYMLCGVSDAF I+GLQEL
Sbjct: 421 SILTMGVSALVEAKRVTIAAQHGLTDTPKVTIPMTIWWLIPQYMLCGVSDAFAIIGLQEL 480

Query: 481 FYDQMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYF 540
           FYDQMP+SMRSLGAAAYISI+GVGNFLSSA+IS+VQ    AGSG  WL+DNLNR+ LHYF
Sbjct: 481 FYDQMPQSMRSLGAAAYISIIGVGNFLSSAVISIVQ----AGSGGSWLEDNLNRSNLHYF 540

Query: 541 YWVLAALSALNLCAYVWIANGFVYKRVEGNINGDDRMSVEKELSLKNSN--NGRCGDDLI 591
           YWVLAALSALNLC YVWIANGFVYKRV    NGDD         +KNSN  NG  GDD+I
Sbjct: 541 YWVLAALSALNLCGYVWIANGFVYKRVGDKNNGDD---------VKNSNNINGCYGDDMI 586

BLAST of Sgr018742 vs. NCBI nr
Match: XP_011655920.1 (protein NRT1/ PTR FAMILY 5.4 isoform X2 [Cucumis sativus] >KAE8648867.1 hypothetical protein Csa_008874 [Cucumis sativus])

HSP 1 Score: 899.4 bits (2323), Expect = 1.6e-257
Identity = 464/602 (77.08%), Postives = 511/602 (84.88%), Query Frame = 0

Query: 4   KEKAPTP---INVGKKFSDDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSNL 63
           K+K+P     IN+  KF D    S+    + PGGGWKAAIF+I VE+AEQFA IGLSSNL
Sbjct: 3   KQKSPNNVPIINLPNKFPDHPTASNSPT-VRPGGGWKAAIFIIFVEVAEQFASIGLSSNL 62

Query: 64  ITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVL 123
           I YFTTV HEP  +AAK VNNWVGVSAVFPLLGAFVADSLLGRF+TII +S+I+F+GM++
Sbjct: 63  IMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMMV 122

Query: 124 LTLSVSVIGESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNW 183
           LT+S +V+G++ RKA FF+ LYILSVG+GGHRPCVQTFAADQFDE SPEERKKKSSFFNW
Sbjct: 123 LTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNW 182

Query: 184 WYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSPL 243
           WYVGLV GST AVFVVIYVQDNI W +SFG+LAGVL AAI+LFL GVKKYRRQ P+GSPL
Sbjct: 183 WYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPL 242

Query: 244 TRIAQVVVAAARKWRVDETRYGWRVCYE-------DTEGEHMVTTLGRTNQFRVLDKATY 303
           TRIAQVVVAAARKWRVDETR GWR+CYE       D EGEH + TL RTNQFR+LDKAT 
Sbjct: 243 TRIAQVVVAAARKWRVDETRNGWRICYEEDNRAKNDAEGEHNLMTLARTNQFRILDKATL 302

Query: 304 IDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTML 363
           ID+ DE RK+R+PWRL TV EVEEVKLV RLIPVWVSCLMFAVVQAQ+HT+FTKQGSTML
Sbjct: 303 IDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTML 362

Query: 364 RSVGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFIS 423
           RSVGP F LPPASLQGVVGLTILLT+LFYDRVFVPAARNFT HHSGITVLQRIG+GLFIS
Sbjct: 363 RSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFIS 422

Query: 424 ILNMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELF 483
           I  M VSALVEAKRV +AA+H L+DTPKA VPMTIWWLIPQYMLCGVSDAF I+GLQELF
Sbjct: 423 IFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELF 482

Query: 484 YDQMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFY 543
           YDQMPE MRSLGAAAYISI+GVGNFLSSAIISVVQ    AGSG +WL+DNLNR+ LHYFY
Sbjct: 483 YDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQ----AGSGGRWLEDNLNRSNLHYFY 542

Query: 544 WVLAALSALNLCAYVWIANGFVYKRVEGN---INGDDRMSVEKELSLKNSN--NGRCGDD 591
           WVLAALSALNLC YVWIANGFVYKR  GN   I+GDD         +KNSN  NG  GDD
Sbjct: 543 WVLAALSALNLCGYVWIANGFVYKRAGGNRRSISGDDD-------DVKNSNNINGCYGDD 592

BLAST of Sgr018742 vs. NCBI nr
Match: XP_008446958.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.4 [Cucumis melo] >TYK09312.1 protein NRT1/ PTR FAMILY 5.4 [Cucumis melo var. makuwa])

HSP 1 Score: 898.7 bits (2321), Expect = 2.8e-257
Identity = 464/601 (77.20%), Postives = 511/601 (85.02%), Query Frame = 0

Query: 4   KEKAPTP---INVGKKFSDDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSNL 63
           K+K+P     IN+  KF D    S+    + PGGGWKAAIF+I VE+AEQFA IGLSSNL
Sbjct: 3   KQKSPNNVPIINLPNKFPDHPTASNTPT-VRPGGGWKAAIFIIFVEVAEQFASIGLSSNL 62

Query: 64  ITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVL 123
           I YFTTV HEP  +AAK VNNWVGVSAVFPLLGAFVADSLLGRF+TII +S+++F GMV+
Sbjct: 63  IMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMVV 122

Query: 124 LTLSVSVIGESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNW 183
           LT+S +V+G+ HRKA FF+ LYILSVG+GGHRPCVQTFAADQF+E +PEERKKKSSFFNW
Sbjct: 123 LTVSATVVGDDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFNW 182

Query: 184 WYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSPL 243
           WYVGLV GST AVFVVIYVQDNI W +SFG+LAGVL AAI+LFL GVKKYRRQ P+GSPL
Sbjct: 183 WYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPL 242

Query: 244 TRIAQVVVAAARKWRVDETRYGWRVCYEDT-------EGEHMVTTLGRTNQFRVLDKATY 303
           TRIAQVVVAAARKW VDETR+ WRVCYE+        EG+H + TL RTNQFR+LDKAT 
Sbjct: 243 TRIAQVVVAAARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFRILDKATL 302

Query: 304 IDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTML 363
           ID+ DE RK+R+PWRL TV EVEEVKLV RLIPVWVSCLMFAVVQAQ+HT+FTKQGSTML
Sbjct: 303 IDKEDEARKKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTML 362

Query: 364 RSVGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFIS 423
           RSVGP F LPPASLQGVVGLTILLT+LFYDRVFVPAARNFT HHSGITVLQRIG+GLFIS
Sbjct: 363 RSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFIS 422

Query: 424 ILNMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELF 483
           IL M VSALVEAKRV +AA+H L+DTPKA VPMTIWWLIPQYMLCGVSDAF IVGLQELF
Sbjct: 423 ILTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQELF 482

Query: 484 YDQMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFY 543
           YDQMP+ MRSLGAAAYISI+GVGNFLSSAIISVVQ    AGSG +WLDDNLNR+ LHYFY
Sbjct: 483 YDQMPQFMRSLGAAAYISIIGVGNFLSSAIISVVQ----AGSGGRWLDDNLNRSNLHYFY 542

Query: 544 WVLAALSALNLCAYVWIANGFVYKRVEGN--INGDDRMSVEKELSLKNSN--NGRCGDDL 591
           WVLAALSALNLC YVWIANGFVYKRV GN  INGDD         +KNSN  NG  GDD+
Sbjct: 543 WVLAALSALNLCGYVWIANGFVYKRVGGNRSINGDDG-------DVKNSNNINGCYGDDM 591

BLAST of Sgr018742 vs. NCBI nr
Match: XP_031740965.1 (protein NRT1/ PTR FAMILY 5.4 isoform X1 [Cucumis sativus])

HSP 1 Score: 894.8 bits (2311), Expect = 4.0e-256
Identity = 464/603 (76.95%), Postives = 511/603 (84.74%), Query Frame = 0

Query: 4   KEKAPTP---INVGKKFSDDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSNL 63
           K+K+P     IN+  KF D    S+    + PGGGWKAAIF+I VE+AEQFA IGLSSNL
Sbjct: 3   KQKSPNNVPIINLPNKFPDHPTASNSPT-VRPGGGWKAAIFIIFVEVAEQFASIGLSSNL 62

Query: 64  ITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVL 123
           I YFTTV HEP  +AAK VNNWVGVSAVFPLLGAFVADSLLGRF+TII +S+I+F+GM++
Sbjct: 63  IMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMMV 122

Query: 124 LTLSVSVIGESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNW 183
           LT+S +V+G++ RKA FF+ LYILSVG+GGHRPCVQTFAADQFDE SPEERKKKSSFFNW
Sbjct: 123 LTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNW 182

Query: 184 WYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSPL 243
           WYVGLV GST AVFVVIYVQDNI W +SFG+LAGVL AAI+LFL GVKKYRRQ P+GSPL
Sbjct: 183 WYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPL 242

Query: 244 TRIAQVVVAAARKWRVDETRYGWRVCYE-------DTEGEHMVTTLGRTNQF-RVLDKAT 303
           TRIAQVVVAAARKWRVDETR GWR+CYE       D EGEH + TL RTNQF R+LDKAT
Sbjct: 243 TRIAQVVVAAARKWRVDETRNGWRICYEEDNRAKNDAEGEHNLMTLARTNQFSRILDKAT 302

Query: 304 YIDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTM 363
            ID+ DE RK+R+PWRL TV EVEEVKLV RLIPVWVSCLMFAVVQAQ+HT+FTKQGSTM
Sbjct: 303 LIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 362

Query: 364 LRSVGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFI 423
           LRSVGP F LPPASLQGVVGLTILLT+LFYDRVFVPAARNFT HHSGITVLQRIG+GLFI
Sbjct: 363 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 422

Query: 424 SILNMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQEL 483
           SI  M VSALVEAKRV +AA+H L+DTPKA VPMTIWWLIPQYMLCGVSDAF I+GLQEL
Sbjct: 423 SIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQEL 482

Query: 484 FYDQMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYF 543
           FYDQMPE MRSLGAAAYISI+GVGNFLSSAIISVVQ    AGSG +WL+DNLNR+ LHYF
Sbjct: 483 FYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQ----AGSGGRWLEDNLNRSNLHYF 542

Query: 544 YWVLAALSALNLCAYVWIANGFVYKRVEGN---INGDDRMSVEKELSLKNSN--NGRCGD 591
           YWVLAALSALNLC YVWIANGFVYKR  GN   I+GDD         +KNSN  NG  GD
Sbjct: 543 YWVLAALSALNLCGYVWIANGFVYKRAGGNRRSISGDDD-------DVKNSNNINGCYGD 593

BLAST of Sgr018742 vs. ExPASy Swiss-Prot
Match: Q9M1I2 (Protein NRT1/ PTR FAMILY 5.4 OS=Arabidopsis thaliana OX=3702 GN=NPF5.4 PE=2 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 1.2e-181
Identity = 309/548 (56.39%), Postives = 421/548 (76.82%), Query Frame = 0

Query: 34  GGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLG 93
           GGW AA+F+I VEIAE+FAF GL+SNLIT+ T  L + TA AAK++N W+GVS +FP+LG
Sbjct: 14  GGWNAALFIIVVEIAERFAFYGLASNLITFLTNELGQSTATAAKNINTWIGVSCMFPILG 73

Query: 94  AFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVIGESHRKAAFFVALYILSVGEGGHRP 153
           AF+ADS+LGRF+T++ +S IY LG+V+L LSV+V+    R+  FF+ALY+++VGEGGH+P
Sbjct: 74  AFLADSILGRFKTVLLTSFIYLLGIVMLPLSVTVVARRMREKVFFMALYVMAVGEGGHKP 133

Query: 154 CVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLA 213
           CV TFAADQF E + EE+  K+SFFN+WY+ +V+ S++AV  +I++Q+ +SW++ F ++A
Sbjct: 134 CVMTFAADQFGEANAEEKAAKTSFFNYWYMAIVLASSIAVLALIFIQERVSWSLGFSIIA 193

Query: 214 GVLGAAIVLFLGGVKKYRRQGPLGSPLTRIAQVVVAAARKWRVDETRYGWRVCYEDTEGE 273
           G +  AIV+FL G+ KYR+Q P+GSP TR+AQV+VAA +KWR+  TR+ + +CYE+ E E
Sbjct: 194 GSVVIAIVIFLIGIPKYRKQVPVGSPFTRVAQVMVAALKKWRLSSTRHHYGLCYEE-EDE 253

Query: 274 H--------MVTTLGRTNQFRVLDKATYIDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLI 333
           H         V  L RTNQFR LDKAT IDE+D   K RNPWRL TV++VEEVKL+ RLI
Sbjct: 254 HKLESTNSNQVYLLARTNQFRFLDKATIIDEIDH-NKNRNPWRLCTVNQVEEVKLILRLI 313

Query: 334 PVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPPASLQGVVGLTILLTILFYDRV 393
           P+W+S +MF     QL+T+F KQGS M R++G  F +PPA+ Q +VG+TIL+ I  YDRV
Sbjct: 314 PIWISLIMFCATLTQLNTFFLKQGSMMDRTIGNHFTIPPAAFQSIVGVTILILIPLYDRV 373

Query: 394 FVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVEAKRVAVAADHHLTDTPKAVVP 453
           FVP  R  T HHSGIT LQRIG+GLF++  NM +  LVEAKR+ VA DH L D+PK VVP
Sbjct: 374 FVPMVRKITNHHSGITSLQRIGVGLFVATFNMVICGLVEAKRLKVARDHGLIDSPKEVVP 433

Query: 454 MTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSLGAAAYISIVGVGNFLSSAIIS 513
           M+  WL+PQY+L G+ D FTIVG+QELFYDQMPE+MRS+GAA +IS+VGVG+F+S+ IIS
Sbjct: 434 MSSLWLLPQYILVGIGDVFTIVGMQELFYDQMPETMRSIGAAIFISVVGVGSFVSTGIIS 493

Query: 514 VVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNLCAYVWIANGFVYKRVEGNING 573
            VQ ++    G +WL +NLNRA L Y+YW++A+L+A++LC Y++IAN F+YK+++   + 
Sbjct: 494 TVQTIS-KSHGEEWLVNNLNRAHLDYYYWIIASLNAVSLCFYLFIANHFLYKKLQ---DK 553

BLAST of Sgr018742 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 6.3e-135
Identity = 253/543 (46.59%), Postives = 352/543 (64.83%), Query Frame = 0

Query: 34  GGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLG 93
           GGW++A F+I VE+AE+FA+ G+SSNLITY T  L + TA AA +VN W G +++ PLLG
Sbjct: 32  GGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQSTAAAAANVNAWSGTASLLPLLG 91

Query: 94  AFVADSLLGRFRTIIFSSVIYFLGMVLLTLS------------VSVIGESHRKAAFFVAL 153
           AFVADS LGRFRTI+ +S +Y +G+ +LTLS            +S      +   FF AL
Sbjct: 92  AFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSDCKVSNLLSSCSPRFQVITFFSAL 151

Query: 154 YILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGSTLAVFVVIYVQD 213
           Y++++ +GGH+PCVQ F ADQFDE  PEE K KSSFFNWWY G+  G+   ++V+ Y+QD
Sbjct: 152 YLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSFFNWWYFGMCFGTLTTLWVLNYIQD 211

Query: 214 NISWAVSFGVLAGVLGAAIVLFLGGVKKY----RRQGPLGSPLTRIAQVVVAAARKWRVD 273
           N+SWA+ FG+    +  A+V+ L G   Y    RR+    SP  RI  V VAA + W V 
Sbjct: 212 NLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQ--SPFVRIGNVYVAAVKNWSVS 271

Query: 274 ETRYGWRVCYEDTEGEHMVTTLGRTNQFRVLDKATYIDEVDEGRKRRNPWRLRTVSEVEE 333
                     E+  G   + +   + QF  L+KA                   ++ E+EE
Sbjct: 272 ALDV---AAAEERLG---LVSCSSSQQFSFLNKALVAKNGS-----------CSIDELEE 331

Query: 334 VKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPPASLQGVVGLTILL 393
            K V RL P+W++CL++AVV AQ  T+FTKQG+TM RS+ P + + PA+LQ  + L+I++
Sbjct: 332 AKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKISPATLQSFISLSIVI 391

Query: 394 TILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVEAKRVAVAADHHLT 453
            I  YDRV +P AR+FT    GIT+LQRIG G+F+S L M V+ALVE KR+  AAD+ L 
Sbjct: 392 FIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAALVEMKRLKTAADYGLV 451

Query: 454 DTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSLGAAAYISIVGVGN 513
           D+P A VPM++WWL+PQY+L G++D F +VGLQE FYDQ+P  +RS+G A Y+SI G+GN
Sbjct: 452 DSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELRSVGLALYLSIFGIGN 511

Query: 514 FLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNLCAYVWIANGFVYK 561
           FLSS +IS+++  T       W  +NLN+A L YFYW+LA LS + L +Y+++A  +V K
Sbjct: 512 FLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFIGLASYLYVAKSYVSK 555

BLAST of Sgr018742 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 457.2 bits (1175), Expect = 2.8e-127
Identity = 241/556 (43.35%), Postives = 351/556 (63.13%), Query Frame = 0

Query: 19  DDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMAAKH 78
           D R ++ R +     G W+AA+F+I VE+AE+FA+ G+ SNLI+Y T  L E TA+AA +
Sbjct: 19  DHRGLAARRSNT---GRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAVAAAN 78

Query: 79  VNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVI---------G 138
           VN W G++ + P+LGAFVAD+ LGR+RTII SS+IY LG+  LTLS  +I          
Sbjct: 79  VNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEVTSST 138

Query: 139 ESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGS 198
            S     FF +LY++++G+ GH+PCVQ F ADQFDE   +E+  +SSFFNWWY+ L  G 
Sbjct: 139 SSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWYLSLSAGI 198

Query: 199 TLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKY----RRQGPLGSPLTRIAQ 258
             A+ VV+Y+Q+  SWA  FG+    +  ++VLF+ G + Y    RR     +P TRI +
Sbjct: 199 CFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRIGR 258

Query: 259 VVVAAARKWRVDETRYGWRVCYEDTEGEHMVTTLGRTNQFRVLDKATYI-DEVDEGRKRR 318
           V   A +  R+  +     +C  + E      T      F   +KA  + ++  +G    
Sbjct: 259 VFFVALKNQRLSSS----DLCKVELE----ANTSPEKQSF--FNKALLVPNDSSQGENAS 318

Query: 319 NPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPP 378
                   S+VE+   + RLIPVW + L +A+  AQ  T+FTKQG TM R++ P   +PP
Sbjct: 319 KS------SDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPP 378

Query: 379 ASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVE 438
           ASLQ  +G++I+L +  YDRVFVP AR  T+   GIT L+RIG G+ +S + M ++ALVE
Sbjct: 379 ASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVE 438

Query: 439 AKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSL 498
            KR+  A +H L D P+A +PM+IWWLIPQY+L G++D +T+VG+QE FY Q+P  +RS+
Sbjct: 439 FKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSI 498

Query: 499 GAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNL 558
           G A Y+S +GVG+ LSS +IS++   TG  +G  W + NLNRA L YFYW+LA +SA+  
Sbjct: 499 GLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGF 555

Query: 559 CAYVWIANGFVYKRVE 561
             +++I+  ++Y+RV+
Sbjct: 559 FTFLFISKSYIYRRVD 555

BLAST of Sgr018742 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 455.7 bits (1171), Expect = 8.2e-127
Identity = 238/540 (44.07%), Postives = 344/540 (63.70%), Query Frame = 0

Query: 34  GGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLG 93
           G W+AA F+I VE+AE+FA  G+ SNLI+Y T  L + TA+AA +VN W G+S + PLLG
Sbjct: 31  GRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQSTAVAAANVNAWSGISTILPLLG 90

Query: 94  AFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVI---------GESHRKAAFFVALYIL 153
           AFVAD+ LGR+ TII +S IY LG+  LTLS  +I           S   A FF +LY++
Sbjct: 91  AFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNNTEVTSSPSSFLNALFFFSLYLV 150

Query: 154 SVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGSTLAVFVVIYVQDNIS 213
           ++G+ GH+PCVQ F ADQFDE +P+E   +SSFFNWWY+ +  G  LA+ VV+Y+Q+N+S
Sbjct: 151 AIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVS 210

Query: 214 WAVSFGVLAGVLGAAIVLFLGGVKKYR----RQGPLGSPLTRIAQVVVAAARKWRVDETR 273
           WA+ FG+    +  ++VLF+ G K YR    RQ    +P TRI +V   A +  R++ + 
Sbjct: 211 WALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSSD 270

Query: 274 YGWRVCYEDTEGEHMVTTLGRTNQFRVLDKATYIDEVDEGRKRRNPWRLRTVSEVEEVKL 333
                  E    +     L   N+  ++   +  DE +   K R         +VE+   
Sbjct: 271 LCKVELIEANRSQESPEELSFLNKALLVPNDS--DEGEVACKSR---------DVEDATA 330

Query: 334 VGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPPASLQGVVGLTILLTIL 393
           + RLIPVW++ L +A+  AQ  T+FTKQG TM R++ P   +PPASLQ ++ ++I+L + 
Sbjct: 331 LVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPASLQVLISISIVLFVP 390

Query: 394 FYDRVFVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVEAKRVAVAADHHLTDTP 453
            YDRV VP  R+ T+   GIT L+RIG G+ ++ L M V+ALVE+KR+  A ++ L D P
Sbjct: 391 IYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESKRLETAKEYGLIDQP 450

Query: 454 KAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSLGAAAYISIVGVGNFLS 513
           K  +PM+IWWL PQYML G++D  T+VG+QE FY Q+P  +RSLG A Y+S +GVG+ LS
Sbjct: 451 KTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGLAIYLSAMGVGSLLS 510

Query: 514 SAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNLCAYVWIANGFVYKRVE 561
           S +I ++   TG  +G  W + NLNRA L YFYW+LA +SA+    +++I+  ++Y+RV+
Sbjct: 511 SLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFTFLFISKSYIYRRVD 559

BLAST of Sgr018742 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 5.9e-125
Identity = 244/569 (42.88%), Postives = 356/569 (62.57%), Query Frame = 0

Query: 22  NVSDR--HNKLPPG----GGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMA 81
           +VSD   H  LP G    GGW++A ++I VE+ E+FA+ G+ SNLITY T  L + TA A
Sbjct: 14  SVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPLGQSTATA 73

Query: 82  AKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVI------- 141
           A +VN W G +++ P+LGAF+AD+ LGR+RTI+ +S+IY LG+ LLTLS  +I       
Sbjct: 74  AVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILILMGLSEQ 133

Query: 142 GESHRKAA----------FFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFF 201
            + +R A+          FF +LY++++G+GGH+PCVQ F ADQFD G P+ER  + SFF
Sbjct: 134 RQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKERISRGSFF 193

Query: 202 NWWYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYR-----RQ 261
           NWW++ L  G TL++ VV+YVQDN++WA+ FG+    +  A+ LFL G K YR     R+
Sbjct: 194 NWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYRYPRGDRE 253

Query: 262 GPLGSPLTRIAQVVVAAARKWRVDETRYGWRV--CYEDTEGEHMVTTLGRTNQFRVLDKA 321
           G   +   RI +V + A +  ++  T  G      Y+  +G           Q   L KA
Sbjct: 254 GK-NNAFARIGRVFLVAFKNRKLKLTHSGQLEVGSYKKCKG-----------QLEFLAKA 313

Query: 322 TYIDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGST 381
                   G     P   R   +VE+   + RLIP+W++ ++  +  AQ  T+FTKQG T
Sbjct: 314 LL-----PGEGGVEPCSSR---DVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVT 373

Query: 382 MLRSVGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLF 441
           + R + P F +PPAS Q ++GL+I +++  Y+RVF+P AR  T+  SGIT+LQRIG G+ 
Sbjct: 374 VDRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMV 433

Query: 442 ISILNMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQE 501
           +S LNM V+ALVE KR+  A +H L D P A +PM+IWW +PQY+L G+ D F++VG QE
Sbjct: 434 LSSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQE 493

Query: 502 LFYDQMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHY 561
            FYDQ+P  +RS+G A  +S +G+ +FLS  +I+V+   TG   G  W + NLNRA + Y
Sbjct: 494 FFYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDY 553

BLAST of Sgr018742 vs. ExPASy TrEMBL
Match: A0A6J1CEH9 (protein NRT1/ PTR FAMILY 5.4 OS=Momordica charantia OX=3673 GN=LOC111009996 PE=3 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 9.3e-267
Identity = 474/584 (81.16%), Postives = 520/584 (89.04%), Query Frame = 0

Query: 16  KFSDDR-NVSDRHNKLP--PGGGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPT 75
           KFS DR N+SDRHN+ P  PGGGWKAAIFVI VEIAEQFAFIGLSSNLI YFTTVL EPT
Sbjct: 5   KFSGDRNNLSDRHNRPPPAPGGGWKAAIFVICVEIAEQFAFIGLSSNLIMYFTTVLGEPT 64

Query: 76  AMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVIGESH 135
           A AAK VNNW GVSAVFP+LGAFVADS LGRF+TII S++IYFLGMVLL++SVS +G  H
Sbjct: 65  AAAAKQVNNWAGVSAVFPILGAFVADSFLGRFKTIIISTLIYFLGMVLLSVSVSWVGSGH 124

Query: 136 RKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGSTLA 195
           RKA   VALYI+SVGEGGHRPCVQTFAADQFDEGSPEER+KKSSFFNWW+VGLVVGSTLA
Sbjct: 125 RKAVVLVALYIMSVGEGGHRPCVQTFAADQFDEGSPEERRKKSSFFNWWFVGLVVGSTLA 184

Query: 196 VFVVIYVQDNIS--WAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSPLTRIAQVVVAA 255
           VF+VIYVQDN++  W +SFGVLAGVL AAIVLFL GVKKYR+QGPLGSPLT IAQVVVAA
Sbjct: 185 VFLVIYVQDNMATGWGLSFGVLAGVLAAAIVLFLAGVKKYRQQGPLGSPLTTIAQVVVAA 244

Query: 256 ARKWRVDETRYGWRVCYEDT----EGEHMVTTLGRTNQFRVLDKATYIDEVDEGRKRRNP 315
           ARKWRVDETR+GWRVCYED     EG H++TTLG+TNQFR+L+KAT ++E DE RK R+ 
Sbjct: 245 ARKWRVDETRHGWRVCYEDNDAHHEGRHVLTTLGQTNQFRILNKATLMEEADEKRKVRDR 304

Query: 316 WRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPPAS 375
           WR+ TV EVEEVKLV RL+PVWVSCLMF VVQAQLHT+FTKQGSTMLRS+GP F LPPAS
Sbjct: 305 WRVSTVGEVEEVKLVVRLVPVWVSCLMFGVVQAQLHTFFTKQGSTMLRSIGPHFQLPPAS 364

Query: 376 LQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVEAK 435
           LQGVVGLTILLT+LFYDRVFVPAARNFT HHSGITVLQRIGIGLFISILNM+VSALVE K
Sbjct: 365 LQGVVGLTILLTVLFYDRVFVPAARNFTAHHSGITVLQRIGIGLFISILNMAVSALVETK 424

Query: 436 RVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSLGA 495
           RVAVAA+H LTD PKAVVPMTIWWLIPQYMLC +SDAF IVGLQELFYDQMPESMRSLGA
Sbjct: 425 RVAVAANHGLTDIPKAVVPMTIWWLIPQYMLCXISDAFAIVGLQELFYDQMPESMRSLGA 484

Query: 496 AAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNLCA 555
           AA ISI+GVGNFLSSA+ISVVQA T A  GRKWL+DNLNR+ LHYFYW+LAALSALNLC 
Sbjct: 485 AANISIIGVGNFLSSAVISVVQAATVARGGRKWLEDNLNRSNLHYFYWILAALSALNLCG 544

Query: 556 YVWIANGFVYKRVEGNINGDDRMSVEKELSLKNSNNGRCGDDLI 591
           YVW+ANGFVYKRV+  + G++   VEKE+S KNSNNG  GDD+I
Sbjct: 545 YVWMANGFVYKRVDQEL-GNNINGVEKEVSFKNSNNGCHGDDMI 587

BLAST of Sgr018742 vs. ExPASy TrEMBL
Match: A0A5D3CDG7 (Protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003090 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.4e-257
Identity = 464/601 (77.20%), Postives = 511/601 (85.02%), Query Frame = 0

Query: 4   KEKAPTP---INVGKKFSDDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSNL 63
           K+K+P     IN+  KF D    S+    + PGGGWKAAIF+I VE+AEQFA IGLSSNL
Sbjct: 3   KQKSPNNVPIINLPNKFPDHPTASNTPT-VRPGGGWKAAIFIIFVEVAEQFASIGLSSNL 62

Query: 64  ITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVL 123
           I YFTTV HEP  +AAK VNNWVGVSAVFPLLGAFVADSLLGRF+TII +S+++F GMV+
Sbjct: 63  IMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMVV 122

Query: 124 LTLSVSVIGESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNW 183
           LT+S +V+G+ HRKA FF+ LYILSVG+GGHRPCVQTFAADQF+E +PEERKKKSSFFNW
Sbjct: 123 LTVSATVVGDDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFNW 182

Query: 184 WYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSPL 243
           WYVGLV GST AVFVVIYVQDNI W +SFG+LAGVL AAI+LFL GVKKYRRQ P+GSPL
Sbjct: 183 WYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPL 242

Query: 244 TRIAQVVVAAARKWRVDETRYGWRVCYEDT-------EGEHMVTTLGRTNQFRVLDKATY 303
           TRIAQVVVAAARKW VDETR+ WRVCYE+        EG+H + TL RTNQFR+LDKAT 
Sbjct: 243 TRIAQVVVAAARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFRILDKATL 302

Query: 304 IDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTML 363
           ID+ DE RK+R+PWRL TV EVEEVKLV RLIPVWVSCLMFAVVQAQ+HT+FTKQGSTML
Sbjct: 303 IDKEDEARKKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTML 362

Query: 364 RSVGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFIS 423
           RSVGP F LPPASLQGVVGLTILLT+LFYDRVFVPAARNFT HHSGITVLQRIG+GLFIS
Sbjct: 363 RSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFIS 422

Query: 424 ILNMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELF 483
           IL M VSALVEAKRV +AA+H L+DTPKA VPMTIWWLIPQYMLCGVSDAF IVGLQELF
Sbjct: 423 ILTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQELF 482

Query: 484 YDQMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFY 543
           YDQMP+ MRSLGAAAYISI+GVGNFLSSAIISVVQ    AGSG +WLDDNLNR+ LHYFY
Sbjct: 483 YDQMPQFMRSLGAAAYISIIGVGNFLSSAIISVVQ----AGSGGRWLDDNLNRSNLHYFY 542

Query: 544 WVLAALSALNLCAYVWIANGFVYKRVEGN--INGDDRMSVEKELSLKNSN--NGRCGDDL 591
           WVLAALSALNLC YVWIANGFVYKRV GN  INGDD         +KNSN  NG  GDD+
Sbjct: 543 WVLAALSALNLCGYVWIANGFVYKRVGGNRSINGDDG-------DVKNSNNINGCYGDDM 591

BLAST of Sgr018742 vs. ExPASy TrEMBL
Match: A0A1S3BFS1 (protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo OX=3656 GN=LOC103489513 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.4e-257
Identity = 464/601 (77.20%), Postives = 511/601 (85.02%), Query Frame = 0

Query: 4   KEKAPTP---INVGKKFSDDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSNL 63
           K+K+P     IN+  KF D    S+    + PGGGWKAAIF+I VE+AEQFA IGLSSNL
Sbjct: 3   KQKSPNNVPIINLPNKFPDHPTASNTPT-VRPGGGWKAAIFIIFVEVAEQFASIGLSSNL 62

Query: 64  ITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVL 123
           I YFTTV HEP  +AAK VNNWVGVSAVFPLLGAFVADSLLGRF+TII +S+++F GMV+
Sbjct: 63  IMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMVV 122

Query: 124 LTLSVSVIGESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNW 183
           LT+S +V+G+ HRKA FF+ LYILSVG+GGHRPCVQTFAADQF+E +PEERKKKSSFFNW
Sbjct: 123 LTVSATVVGDDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFNW 182

Query: 184 WYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSPL 243
           WYVGLV GST AVFVVIYVQDNI W +SFG+LAGVL AAI+LFL GVKKYRRQ P+GSPL
Sbjct: 183 WYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPL 242

Query: 244 TRIAQVVVAAARKWRVDETRYGWRVCYEDT-------EGEHMVTTLGRTNQFRVLDKATY 303
           TRIAQVVVAAARKW VDETR+ WRVCYE+        EG+H + TL RTNQFR+LDKAT 
Sbjct: 243 TRIAQVVVAAARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFRILDKATL 302

Query: 304 IDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTML 363
           ID+ DE RK+R+PWRL TV EVEEVKLV RLIPVWVSCLMFAVVQAQ+HT+FTKQGSTML
Sbjct: 303 IDKEDEARKKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTML 362

Query: 364 RSVGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFIS 423
           RSVGP F LPPASLQGVVGLTILLT+LFYDRVFVPAARNFT HHSGITVLQRIG+GLFIS
Sbjct: 363 RSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFIS 422

Query: 424 ILNMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELF 483
           IL M VSALVEAKRV +AA+H L+DTPKA VPMTIWWLIPQYMLCGVSDAF IVGLQELF
Sbjct: 423 ILTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQELF 482

Query: 484 YDQMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFY 543
           YDQMP+ MRSLGAAAYISI+GVGNFLSSAIISVVQ    AGSG +WLDDNLNR+ LHYFY
Sbjct: 483 YDQMPQFMRSLGAAAYISIIGVGNFLSSAIISVVQ----AGSGGRWLDDNLNRSNLHYFY 542

Query: 544 WVLAALSALNLCAYVWIANGFVYKRVEGN--INGDDRMSVEKELSLKNSN--NGRCGDDL 591
           WVLAALSALNLC YVWIANGFVYKRV GN  INGDD         +KNSN  NG  GDD+
Sbjct: 543 WVLAALSALNLCGYVWIANGFVYKRVGGNRSINGDDG-------DVKNSNNINGCYGDDM 591

BLAST of Sgr018742 vs. ExPASy TrEMBL
Match: A0A5A7SVT8 (Protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007820 PE=3 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 3.3e-256
Identity = 464/602 (77.08%), Postives = 511/602 (84.88%), Query Frame = 0

Query: 4   KEKAPTP---INVGKKFSDDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSNL 63
           K+K+P     IN+  KF D    S+    + PGGGWKAAIF+I VE+AEQFA IGLSSNL
Sbjct: 3   KQKSPNNVPIINLPNKFPDHPTASNTPT-VRPGGGWKAAIFIIFVEVAEQFASIGLSSNL 62

Query: 64  ITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVL 123
           I YFTTV HEP  +AAK VNNWVGVSAVFPLLGAFVADSLLGRF+TII +S+++F GMV+
Sbjct: 63  IMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMVV 122

Query: 124 LTLSVSVIGESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNW 183
           LT+S +V+G+ HRKA FF+ LYILSVG+GGHRPCVQTFAADQF+E +PEERKKKSSFFNW
Sbjct: 123 LTVSATVVGDDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFNW 182

Query: 184 WYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSPL 243
           WYVGLV GST AVFVVIYVQDNI W +SFG+LAGVL AAI+LFL GVKKYRRQ P+GSPL
Sbjct: 183 WYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPL 242

Query: 244 TRIAQVVVAAARKWRVDETRYGWRVCYEDT-------EGEHMVTTLGRTNQF-RVLDKAT 303
           TRIAQVVVAAARKW VDETR+ WRVCYE+        EG+H + TL RTNQF R+LDKAT
Sbjct: 243 TRIAQVVVAAARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFSRILDKAT 302

Query: 304 YIDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTM 363
            ID+ DE RK+R+PWRL TV EVEEVKLV RLIPVWVSCLMFAVVQAQ+HT+FTKQGSTM
Sbjct: 303 LIDKEDEARKKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 362

Query: 364 LRSVGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFI 423
           LRSVGP F LPPASLQGVVGLTILLT+LFYDRVFVPAARNFT HHSGITVLQRIG+GLFI
Sbjct: 363 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 422

Query: 424 SILNMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQEL 483
           SIL M VSALVEAKRV +AA+H L+DTPKA VPMTIWWLIPQYMLCGVSDAF IVGLQEL
Sbjct: 423 SILTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQEL 482

Query: 484 FYDQMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYF 543
           FYDQMP+ MRSLGAAAYISI+GVGNFLSSAIISVVQ    AGSG +WLDDNLNR+ LHYF
Sbjct: 483 FYDQMPQFMRSLGAAAYISIIGVGNFLSSAIISVVQ----AGSGGRWLDDNLNRSNLHYF 542

Query: 544 YWVLAALSALNLCAYVWIANGFVYKRVEGN--INGDDRMSVEKELSLKNSN--NGRCGDD 591
           YWVLAALSALNLC YVWIANGFVYKRV GN  INGDD         +KNSN  NG  GDD
Sbjct: 543 YWVLAALSALNLCGYVWIANGFVYKRVGGNRSINGDDG-------DVKNSNNINGCYGDD 592

BLAST of Sgr018742 vs. ExPASy TrEMBL
Match: A0A6J1GWY8 (protein NRT1/ PTR FAMILY 5.4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458199 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 3.6e-250
Identity = 446/595 (74.96%), Postives = 502/595 (84.37%), Query Frame = 0

Query: 4   KEKAPTP-INVGKKFSDDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSNLIT 63
           +EK+P P +++  K  D RN+SDR ++ PP GGWKAAIFVI VE+AEQFAFIGLSSNLI 
Sbjct: 3   EEKSPAPLLHLPTKLPDHRNLSDRPSQ-PPAGGWKAAIFVIFVEVAEQFAFIGLSSNLIM 62

Query: 64  YFTTVLHEPTAMAAKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVLLT 123
           YFTTV HEPTA AAK VNNW GVSAVFP+LGAFVADSLLGRF+TIIFSS+IY LGMVLLT
Sbjct: 63  YFTTVFHEPTATAAKMVNNWSGVSAVFPILGAFVADSLLGRFKTIIFSSLIYCLGMVLLT 122

Query: 124 LSVSVIGESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNWWY 183
           LS +VIG  HRK  FF ALYILSVGEGGHRPCVQTFAADQFDE +PE+RK+KSSFFNWWY
Sbjct: 123 LSATVIGAPHRKPVFFFALYILSVGEGGHRPCVQTFAADQFDEETPEQRKRKSSFFNWWY 182

Query: 184 VGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYRRQGPLGSPLTR 243
           VGLVVGSTLAVF+VIYVQDNI W +SFG+LAGVL AA++LFL GVK YRR  P+GSP+TR
Sbjct: 183 VGLVVGSTLAVFLVIYVQDNIGWGLSFGILAGVLAAALLLFLCGVKVYRRHIPVGSPMTR 242

Query: 244 IAQVVVAAARKWRVDETRYGWRVCYE-------DTEGEHMVTTLGRTNQFRVLDKATYID 303
           IAQVVVAAARKWRVD TR  WRVCYE       + EG+H   TL R +QF +LDKAT ID
Sbjct: 243 IAQVVVAAARKWRVDNTRQEWRVCYEEDSHAKNEDEGQHKPMTLDRRSQFGILDKATLID 302

Query: 304 EVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTMLRS 363
           + D+ RK+R+PWRL TV+EVEEVK++GRLIPVW SCLMFAVVQAQ+HT+FTKQGSTMLRS
Sbjct: 303 DEDKARKKRDPWRLSTVAEVEEVKMLGRLIPVWFSCLMFAVVQAQIHTFFTKQGSTMLRS 362

Query: 364 VGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFISIL 423
           +GP F +PPASLQGVVGLTILLT+LFYDRVFVP+AR FT HHSGITVLQRIGIGLFISIL
Sbjct: 363 IGPHFQIPPASLQGVVGLTILLTVLFYDRVFVPSARKFTGHHSGITVLQRIGIGLFISIL 422

Query: 424 NMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELFYD 483
           NM  SALVEAKRVAVAA+H L DTPK  VPMTIWWLIPQYMLCGVSDAF +VGLQELFYD
Sbjct: 423 NMVASALVEAKRVAVAAEHGLIDTPKVTVPMTIWWLIPQYMLCGVSDAFAVVGLQELFYD 482

Query: 484 QMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFYWV 543
           QMPESMRS+G+AAYIS++G+GNFLS+AIIS VQ    A S  KWL DNLNR++L YFYWV
Sbjct: 483 QMPESMRSIGSAAYISVIGIGNFLSTAIISAVQ----AASRHKWLVDNLNRSKLQYFYWV 542

Query: 544 LAALSALNLCAYVWIANGFVYKRVEGNINGDDRMSVEKELSLKNSNNGRCGDDLI 591
           LA LS LNLC Y+W+ANG+VYKRV G  +G+D          KNSN G  GDD+I
Sbjct: 543 LAGLSGLNLCCYIWVANGYVYKRVGGK-DGEDHG--------KNSNKGGYGDDII 583

BLAST of Sgr018742 vs. TAIR 10
Match: AT3G54450.1 (Major facilitator superfamily protein )

HSP 1 Score: 637.9 bits (1644), Expect = 8.3e-183
Identity = 309/548 (56.39%), Postives = 421/548 (76.82%), Query Frame = 0

Query: 34  GGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLG 93
           GGW AA+F+I VEIAE+FAF GL+SNLIT+ T  L + TA AAK++N W+GVS +FP+LG
Sbjct: 14  GGWNAALFIIVVEIAERFAFYGLASNLITFLTNELGQSTATAAKNINTWIGVSCMFPILG 73

Query: 94  AFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVIGESHRKAAFFVALYILSVGEGGHRP 153
           AF+ADS+LGRF+T++ +S IY LG+V+L LSV+V+    R+  FF+ALY+++VGEGGH+P
Sbjct: 74  AFLADSILGRFKTVLLTSFIYLLGIVMLPLSVTVVARRMREKVFFMALYVMAVGEGGHKP 133

Query: 154 CVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLA 213
           CV TFAADQF E + EE+  K+SFFN+WY+ +V+ S++AV  +I++Q+ +SW++ F ++A
Sbjct: 134 CVMTFAADQFGEANAEEKAAKTSFFNYWYMAIVLASSIAVLALIFIQERVSWSLGFSIIA 193

Query: 214 GVLGAAIVLFLGGVKKYRRQGPLGSPLTRIAQVVVAAARKWRVDETRYGWRVCYEDTEGE 273
           G +  AIV+FL G+ KYR+Q P+GSP TR+AQV+VAA +KWR+  TR+ + +CYE+ E E
Sbjct: 194 GSVVIAIVIFLIGIPKYRKQVPVGSPFTRVAQVMVAALKKWRLSSTRHHYGLCYEE-EDE 253

Query: 274 H--------MVTTLGRTNQFRVLDKATYIDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLI 333
           H         V  L RTNQFR LDKAT IDE+D   K RNPWRL TV++VEEVKL+ RLI
Sbjct: 254 HKLESTNSNQVYLLARTNQFRFLDKATIIDEIDH-NKNRNPWRLCTVNQVEEVKLILRLI 313

Query: 334 PVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPPASLQGVVGLTILLTILFYDRV 393
           P+W+S +MF     QL+T+F KQGS M R++G  F +PPA+ Q +VG+TIL+ I  YDRV
Sbjct: 314 PIWISLIMFCATLTQLNTFFLKQGSMMDRTIGNHFTIPPAAFQSIVGVTILILIPLYDRV 373

Query: 394 FVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVEAKRVAVAADHHLTDTPKAVVP 453
           FVP  R  T HHSGIT LQRIG+GLF++  NM +  LVEAKR+ VA DH L D+PK VVP
Sbjct: 374 FVPMVRKITNHHSGITSLQRIGVGLFVATFNMVICGLVEAKRLKVARDHGLIDSPKEVVP 433

Query: 454 MTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSLGAAAYISIVGVGNFLSSAIIS 513
           M+  WL+PQY+L G+ D FTIVG+QELFYDQMPE+MRS+GAA +IS+VGVG+F+S+ IIS
Sbjct: 434 MSSLWLLPQYILVGIGDVFTIVGMQELFYDQMPETMRSIGAAIFISVVGVGSFVSTGIIS 493

Query: 514 VVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNLCAYVWIANGFVYKRVEGNING 573
            VQ ++    G +WL +NLNRA L Y+YW++A+L+A++LC Y++IAN F+YK+++   + 
Sbjct: 494 TVQTIS-KSHGEEWLVNNLNRAHLDYYYWIIASLNAVSLCFYLFIANHFLYKKLQ---DK 553

BLAST of Sgr018742 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 482.6 bits (1241), Expect = 4.4e-136
Identity = 253/543 (46.59%), Postives = 352/543 (64.83%), Query Frame = 0

Query: 34  GGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLG 93
           GGW++A F+I VE+AE+FA+ G+SSNLITY T  L + TA AA +VN W G +++ PLLG
Sbjct: 32  GGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQSTAAAAANVNAWSGTASLLPLLG 91

Query: 94  AFVADSLLGRFRTIIFSSVIYFLGMVLLTLS------------VSVIGESHRKAAFFVAL 153
           AFVADS LGRFRTI+ +S +Y +G+ +LTLS            +S      +   FF AL
Sbjct: 92  AFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSDCKVSNLLSSCSPRFQVITFFSAL 151

Query: 154 YILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGSTLAVFVVIYVQD 213
           Y++++ +GGH+PCVQ F ADQFDE  PEE K KSSFFNWWY G+  G+   ++V+ Y+QD
Sbjct: 152 YLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSFFNWWYFGMCFGTLTTLWVLNYIQD 211

Query: 214 NISWAVSFGVLAGVLGAAIVLFLGGVKKY----RRQGPLGSPLTRIAQVVVAAARKWRVD 273
           N+SWA+ FG+    +  A+V+ L G   Y    RR+    SP  RI  V VAA + W V 
Sbjct: 212 NLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQ--SPFVRIGNVYVAAVKNWSVS 271

Query: 274 ETRYGWRVCYEDTEGEHMVTTLGRTNQFRVLDKATYIDEVDEGRKRRNPWRLRTVSEVEE 333
                     E+  G   + +   + QF  L+KA                   ++ E+EE
Sbjct: 272 ALDV---AAAEERLG---LVSCSSSQQFSFLNKALVAKNGS-----------CSIDELEE 331

Query: 334 VKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPPASLQGVVGLTILL 393
            K V RL P+W++CL++AVV AQ  T+FTKQG+TM RS+ P + + PA+LQ  + L+I++
Sbjct: 332 AKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKISPATLQSFISLSIVI 391

Query: 394 TILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVEAKRVAVAADHHLT 453
            I  YDRV +P AR+FT    GIT+LQRIG G+F+S L M V+ALVE KR+  AAD+ L 
Sbjct: 392 FIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAALVEMKRLKTAADYGLV 451

Query: 454 DTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSLGAAAYISIVGVGN 513
           D+P A VPM++WWL+PQY+L G++D F +VGLQE FYDQ+P  +RS+G A Y+SI G+GN
Sbjct: 452 DSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELRSVGLALYLSIFGIGN 511

Query: 514 FLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNLCAYVWIANGFVYK 561
           FLSS +IS+++  T       W  +NLN+A L YFYW+LA LS + L +Y+++A  +V K
Sbjct: 512 FLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFIGLASYLYVAKSYVSK 555

BLAST of Sgr018742 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 457.2 bits (1175), Expect = 2.0e-128
Identity = 241/556 (43.35%), Postives = 351/556 (63.13%), Query Frame = 0

Query: 19  DDRNVSDRHNKLPPGGGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMAAKH 78
           D R ++ R +     G W+AA+F+I VE+AE+FA+ G+ SNLI+Y T  L E TA+AA +
Sbjct: 19  DHRGLAARRSNT---GRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAVAAAN 78

Query: 79  VNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVI---------G 138
           VN W G++ + P+LGAFVAD+ LGR+RTII SS+IY LG+  LTLS  +I          
Sbjct: 79  VNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEVTSST 138

Query: 139 ESHRKAAFFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGS 198
            S     FF +LY++++G+ GH+PCVQ F ADQFDE   +E+  +SSFFNWWY+ L  G 
Sbjct: 139 SSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWYLSLSAGI 198

Query: 199 TLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKY----RRQGPLGSPLTRIAQ 258
             A+ VV+Y+Q+  SWA  FG+    +  ++VLF+ G + Y    RR     +P TRI +
Sbjct: 199 CFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRIGR 258

Query: 259 VVVAAARKWRVDETRYGWRVCYEDTEGEHMVTTLGRTNQFRVLDKATYI-DEVDEGRKRR 318
           V   A +  R+  +     +C  + E      T      F   +KA  + ++  +G    
Sbjct: 259 VFFVALKNQRLSSS----DLCKVELE----ANTSPEKQSF--FNKALLVPNDSSQGENAS 318

Query: 319 NPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPP 378
                   S+VE+   + RLIPVW + L +A+  AQ  T+FTKQG TM R++ P   +PP
Sbjct: 319 KS------SDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPP 378

Query: 379 ASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVE 438
           ASLQ  +G++I+L +  YDRVFVP AR  T+   GIT L+RIG G+ +S + M ++ALVE
Sbjct: 379 ASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVE 438

Query: 439 AKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSL 498
            KR+  A +H L D P+A +PM+IWWLIPQY+L G++D +T+VG+QE FY Q+P  +RS+
Sbjct: 439 FKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSI 498

Query: 499 GAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNL 558
           G A Y+S +GVG+ LSS +IS++   TG  +G  W + NLNRA L YFYW+LA +SA+  
Sbjct: 499 GLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGF 555

Query: 559 CAYVWIANGFVYKRVE 561
             +++I+  ++Y+RV+
Sbjct: 559 FTFLFISKSYIYRRVD 555

BLAST of Sgr018742 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 455.7 bits (1171), Expect = 5.8e-128
Identity = 238/540 (44.07%), Postives = 344/540 (63.70%), Query Frame = 0

Query: 34  GGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMAAKHVNNWVGVSAVFPLLG 93
           G W+AA F+I VE+AE+FA  G+ SNLI+Y T  L + TA+AA +VN W G+S + PLLG
Sbjct: 31  GRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQSTAVAAANVNAWSGISTILPLLG 90

Query: 94  AFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVI---------GESHRKAAFFVALYIL 153
           AFVAD+ LGR+ TII +S IY LG+  LTLS  +I           S   A FF +LY++
Sbjct: 91  AFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNNTEVTSSPSSFLNALFFFSLYLV 150

Query: 154 SVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFFNWWYVGLVVGSTLAVFVVIYVQDNIS 213
           ++G+ GH+PCVQ F ADQFDE +P+E   +SSFFNWWY+ +  G  LA+ VV+Y+Q+N+S
Sbjct: 151 AIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVS 210

Query: 214 WAVSFGVLAGVLGAAIVLFLGGVKKYR----RQGPLGSPLTRIAQVVVAAARKWRVDETR 273
           WA+ FG+    +  ++VLF+ G K YR    RQ    +P TRI +V   A +  R++ + 
Sbjct: 211 WALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSSD 270

Query: 274 YGWRVCYEDTEGEHMVTTLGRTNQFRVLDKATYIDEVDEGRKRRNPWRLRTVSEVEEVKL 333
                  E    +     L   N+  ++   +  DE +   K R         +VE+   
Sbjct: 271 LCKVELIEANRSQESPEELSFLNKALLVPNDS--DEGEVACKSR---------DVEDATA 330

Query: 334 VGRLIPVWVSCLMFAVVQAQLHTYFTKQGSTMLRSVGPRFHLPPASLQGVVGLTILLTIL 393
           + RLIPVW++ L +A+  AQ  T+FTKQG TM R++ P   +PPASLQ ++ ++I+L + 
Sbjct: 331 LVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPASLQVLISISIVLFVP 390

Query: 394 FYDRVFVPAARNFTRHHSGITVLQRIGIGLFISILNMSVSALVEAKRVAVAADHHLTDTP 453
            YDRV VP  R+ T+   GIT L+RIG G+ ++ L M V+ALVE+KR+  A ++ L D P
Sbjct: 391 IYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESKRLETAKEYGLIDQP 450

Query: 454 KAVVPMTIWWLIPQYMLCGVSDAFTIVGLQELFYDQMPESMRSLGAAAYISIVGVGNFLS 513
           K  +PM+IWWL PQYML G++D  T+VG+QE FY Q+P  +RSLG A Y+S +GVG+ LS
Sbjct: 451 KTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGLAIYLSAMGVGSLLS 510

Query: 514 SAIISVVQAVTGAGSGRKWLDDNLNRAQLHYFYWVLAALSALNLCAYVWIANGFVYKRVE 561
           S +I ++   TG  +G  W + NLNRA L YFYW+LA +SA+    +++I+  ++Y+RV+
Sbjct: 511 SLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFTFLFISKSYIYRRVD 559

BLAST of Sgr018742 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 449.5 bits (1155), Expect = 4.2e-126
Identity = 244/569 (42.88%), Postives = 356/569 (62.57%), Query Frame = 0

Query: 22  NVSDR--HNKLPPG----GGWKAAIFVISVEIAEQFAFIGLSSNLITYFTTVLHEPTAMA 81
           +VSD   H  LP G    GGW++A ++I VE+ E+FA+ G+ SNLITY T  L + TA A
Sbjct: 14  SVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPLGQSTATA 73

Query: 82  AKHVNNWVGVSAVFPLLGAFVADSLLGRFRTIIFSSVIYFLGMVLLTLSVSVI------- 141
           A +VN W G +++ P+LGAF+AD+ LGR+RTI+ +S+IY LG+ LLTLS  +I       
Sbjct: 74  AVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILILMGLSEQ 133

Query: 142 GESHRKAA----------FFVALYILSVGEGGHRPCVQTFAADQFDEGSPEERKKKSSFF 201
            + +R A+          FF +LY++++G+GGH+PCVQ F ADQFD G P+ER  + SFF
Sbjct: 134 RQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKERISRGSFF 193

Query: 202 NWWYVGLVVGSTLAVFVVIYVQDNISWAVSFGVLAGVLGAAIVLFLGGVKKYR-----RQ 261
           NWW++ L  G TL++ VV+YVQDN++WA+ FG+    +  A+ LFL G K YR     R+
Sbjct: 194 NWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYRYPRGDRE 253

Query: 262 GPLGSPLTRIAQVVVAAARKWRVDETRYGWRV--CYEDTEGEHMVTTLGRTNQFRVLDKA 321
           G   +   RI +V + A +  ++  T  G      Y+  +G           Q   L KA
Sbjct: 254 GK-NNAFARIGRVFLVAFKNRKLKLTHSGQLEVGSYKKCKG-----------QLEFLAKA 313

Query: 322 TYIDEVDEGRKRRNPWRLRTVSEVEEVKLVGRLIPVWVSCLMFAVVQAQLHTYFTKQGST 381
                   G     P   R   +VE+   + RLIP+W++ ++  +  AQ  T+FTKQG T
Sbjct: 314 LL-----PGEGGVEPCSSR---DVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVT 373

Query: 382 MLRSVGPRFHLPPASLQGVVGLTILLTILFYDRVFVPAARNFTRHHSGITVLQRIGIGLF 441
           + R + P F +PPAS Q ++GL+I +++  Y+RVF+P AR  T+  SGIT+LQRIG G+ 
Sbjct: 374 VDRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMV 433

Query: 442 ISILNMSVSALVEAKRVAVAADHHLTDTPKAVVPMTIWWLIPQYMLCGVSDAFTIVGLQE 501
           +S LNM V+ALVE KR+  A +H L D P A +PM+IWW +PQY+L G+ D F++VG QE
Sbjct: 434 LSSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQE 493

Query: 502 LFYDQMPESMRSLGAAAYISIVGVGNFLSSAIISVVQAVTGAGSGRKWLDDNLNRAQLHY 561
            FYDQ+P  +RS+G A  +S +G+ +FLS  +I+V+   TG   G  W + NLNRA + Y
Sbjct: 494 FFYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDY 553

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138943.11.9e-26681.16protein NRT1/ PTR FAMILY 5.4 [Momordica charantia][more]
XP_038892997.13.0e-25977.67protein NRT1/ PTR FAMILY 5.4 [Benincasa hispida][more]
XP_011655920.11.6e-25777.08protein NRT1/ PTR FAMILY 5.4 isoform X2 [Cucumis sativus] >KAE8648867.1 hypothet... [more]
XP_008446958.12.8e-25777.20PREDICTED: protein NRT1/ PTR FAMILY 5.4 [Cucumis melo] >TYK09312.1 protein NRT1/... [more]
XP_031740965.14.0e-25676.95protein NRT1/ PTR FAMILY 5.4 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9M1I21.2e-18156.39Protein NRT1/ PTR FAMILY 5.4 OS=Arabidopsis thaliana OX=3702 GN=NPF5.4 PE=2 SV=1[more]
Q0WP016.3e-13546.59Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV... [more]
Q8VZE22.8e-12743.35Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV... [more]
Q0WSZ68.2e-12744.07Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV... [more]
Q9SK965.9e-12542.88Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1CEH99.3e-26781.16protein NRT1/ PTR FAMILY 5.4 OS=Momordica charantia OX=3673 GN=LOC111009996 PE=3... [more]
A0A5D3CDG71.4e-25777.20Protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BFS11.4e-25777.20protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo OX=3656 GN=LOC103489513 PE=3 SV=1[more]
A0A5A7SVT83.3e-25677.08Protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A6J1GWY83.6e-25074.96protein NRT1/ PTR FAMILY 5.4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G54450.18.3e-18356.39Major facilitator superfamily protein [more]
AT1G22540.14.4e-13646.59Major facilitator superfamily protein [more]
AT1G72120.12.0e-12843.35Major facilitator superfamily protein [more]
AT1G72125.15.8e-12844.07Major facilitator superfamily protein [more]
AT1G22550.14.2e-12642.88Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 12..562
e-value: 4.0E-176
score: 588.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 21..551
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 104..513
e-value: 1.8E-87
score: 293.8
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 31..560
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availablePANTHERPTHR11654:SF344PROTEIN NRT1/ PTR FAMILY 5.4coord: 31..560
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 93..117
IPR044739NRT1/PTR familyCDDcd17417MFS_NPF5coord: 36..556
e-value: 0.0
score: 626.218

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr018742.1Sgr018742.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0042938 dipeptide transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity