Sgr018488 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr018488
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description1,4-alpha-glucan branching enzyme
Locationtig00153204: 490154 .. 495799 (+)
RNA-Seq ExpressionSgr018488
SyntenySgr018488
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGGCTTCTCCTTCCGTTCTTCCTCCATCAGCTCTTGCTTCTTTGTCACTTTCGGCAAAGAAAAATATCACTACGGTACTTCTCATGGCTTATTTATGGCTTGCCAAGCTCCAGCTCCCCCACCCATTTTCTACTTGGTTTGAGGAGTTTTCTGTTTCATATTGACAGGACTCGAGAAATAAATGTCCTTCATTGAGACAACAAATTGAGCTGATTTTTGGGTCTAGAATGTCGCATCACCGGCAGATGAACCCATTTATGCACCCAATCTCAAATTACAAAAGGGTGATTTTTCTTTCTAATGTTGGTTACTATCATATGTTGAAAAGTGCAAAGTTTTTTCTAAGTGCCTCTGACTATTTCTGAAATGTGCTTCATACTTGCTTCTTTAAGTCATAAGCAGAATCATACCAAATGGTGAATAGTTTCATGTGATTCTATGCTAGTATTATTGGCCAGAGTGCATAAAAAGGAAGAAAATTCCATGTTTATTCAAACTCGAAAGTCAATTTATTCCTTTACTTTTCATTAGTTTAACTGACTGCAAAGACTCCCAAACCAAGTATGAGGTTTCTGAACTAAATTCCAAGTATCTCCTTGGTATGTTCTATGCTCAGATCGGAGACAAATTCTTTGTATGGATTTGTGTTTAGGTTTTGATTTTTTCTCCTCTCTGGACATATGGGAAGCATTATGAGCAGTAATCATTTGAAAATTAGTTGAAAAGTTCTCATTTTTCCACTTGATCTTCATCTTGTAACTCCATTTTACTAGGCACCATTTAATGTTTATTTTGTTAATATGCTACTACATGGTGACTTTCCTTTTCCAGGTCCACAGTTCTGCAATCTCTTCCATCATGACAGATGATACATCAACATTGACATCTGCTGACGAAAGCATGGAAAATATTGGCATCTTGAGCCATGATCCAGGCTTGGAACCCTTCAAAGATCATTTCAAATATAGAGTAAGCAGATATGCGGAACTACTCAACCTTTTAGAGAAGTATGAAGGTGGTCTTGACGAATTCGCACGAGGTGCTTTATTAAACCATATTGAAGACTACTTGCTTTTATGTCATTCATTGAATATCTTGGCAACTACTTCATTTGCGTTTCGTCAATGTTTATTTACTTTACTGTAGTCTGTAGAGCTTGCTCGTATTCATACAGGATGCATCAGTTTTAATGTTGATCAACTTGTTGGATTGAATGATCTAAAATTTAATCAACAGCTTATCATAATTTTCTTCAATTAGCTAATTTCTTCCATGATGCTTTAGGATATCTAAAATTTGGATTTAATAGAGAAGAAGGTGGCATTGAGTATCGTGAATGGGCTCCGGCTGCTCAGTAAGATTTTCTTTTTATTATTTTATTTTTCTAATCATACAAATGTCTATAGTTTAGTTGTTGAGAATGGAAATTATATCTTTTATTATTTTCAGGGAAGCACAAATTGTTGGGGACTTTAATGGATGGGATGGTTCCAAACACCGAATGAAAAAGAATGAATATGGCGTTTGGAGTATTAAACTTCCTGATTGTGGTGACGAACCAGCTATTGCTCACAATTCAAGAGTTAAGTTCCGTTTCAAGCATGGCGATGGAGTTTGGATTGATCGCATCCCTGCTTGGATTAAATATGCCACTGCGGATCCTACAAGATTTGCAGCACCATATGATGGTGTGTATTGGGACCCGCCTCCTTTGGAAAGGTATTGGTCATCCAACGGTTCCTTATAACCCTAATCTGTTTTTCCCATTTCTATAACTGAGATCCACCATCCTATATGGAATTTTAGTTTGAAAGAATCTTTCTTCCACACTCCAAATGATTAGGCCTTGTAAGATTCCTACCACTATCTTTTAGTCTTTGAATCCAAGGAAAATAGTTAATTTGATGTTTCATTATTCTGACCTTAATTCAGGTATGAATTTAAGCATCCACGTCCTACAAAGCCCGAAGCCCCACGGGTATATGACCAGTGGTATACAGGGAATTTGCCGATTTAGTTCTACCACATATTAAAGAAAATAACTACAATACAGTCCAGTTGATGGCTATTATGGAACATTCATATTATGCATCATTTGGTTATCACGTTACCAACTTTTTTGCTGCAAGCAGTAGATCCGGAACTCCTGAGGACCTAAAATATCTTATTGACAAGGCCCATGGCTTGGGTTTACGGGTGTTGATGGATGTTGTTCATAGCCATGCAAGTAACAATGTCACTGATGGCCTTAATGGCTATGATGTCGGTCAAAGTTCCCAAGATTCTTACTTCCACACTGGAGATCGTGGTTACCATAAGCTGTGGGATAGTAGACTCTTCAATTATGCAAATTGGGAAGTTCTGCGTTTCCTTTTATCGAACGTAAGATGGTGGTTGGACGAGTATAAATTTGATGGTTTTCGATTTGATGGAGTAACTTCAATGCTGTATCAACACCATGGAATCAACATGGCTTTCACTGGAAATTATAAAGAGTATTTTAGTGAAGCAACAGACGTTGATGCTGTTGTGTATTTGATGCTAGCCAATAATCTGATTAACAGCATTTTGCCGGATGCAACTGTAATTGCTGAAGATGTTTCGGGCATGCCTGGTCTTGGAAGACCTGTTTCCGAAGGGGGTATTGGTTTTGACTACCGCCTTCAAATGGCCATTCCTGACAAATGGATTGATTACTTGAAGAACAAGAGTGATGAGGAATGGTCCATGGAGGAAATAAGTTGGAACTTGACAAATAGAAGATATTCTGAGAAGTGCGTATCTTATGCTGAGAGTCATGATCAGGTGAGATTCTTATTCTTATATTTTATTTTTATTCTATCTCTATCTTTTGGTAATAGTCAGAGTAATGACAAAATTGATGTTACTAAGTTCTCCCCTTGTGAAAATTGATATCGTTGTACCTTTTTGTCTTTGCACATATTGGACAGGCGATTGTTGGAGACAAGACCATTGCATTTTTGCTGATGGATAAAGAGATGTATTCTGGCATGTCTTGTTTGACAGAGGCATCTCCCATAGTTGAAAGAGGGATTGCACTGCACAAGGTTTCGTTCCTTCCTAATTCATAGACACATCTAATAGGTGGCTTTAAGGGGATTTGGACCCATTACCACATGGTTAAACCCAAATTCTTTTACTGCCGTCGGGCTTGTTTTTCATCATGAAAGTGAAGGTCTCATTTCCAGACTGTCAGTCTGTCCAAATAAAAATAACATACCAGCATTACTACTGCCTGCTGGTCTATGTTTCTGGAGCATAATTGATGACTTCGAGTATTCTTAGATCTTGTTATCTTCCCATCAATTATTATCAGCATATCAGTGATCCTATTTTGAATCTAAACTGTCATTTCTTTCTTTTGCAGATGATACATTTTATAACCATGGCCTTGGGAGGGGAGGGCTACCTTAATTTCATGGGAAATGAGGTGAGCTTAATTTCTTATTCTTCTGATCAATCTCCTAGCATGTCGTTAATATATAAAGTACTATCTCTAATGACTGATGTTAGCTCATGTTTTCACTTTTGTTGGAAATTTCCTTATATTATGATTTTTTTTATTTTAAAAAACGTGCATTTTGGTTTTGAATGATCACATCTTTAACTACCACCATTTTATGTGCAATTTTATCGTTAATGCTTATAGTTCGCGCATCCTGAGTGGATTGACTTCCCACGAGAAGGCAATGAGTGGAGTTATGACAAGTGCAGGCGACAGTGGAATCTGCTTCACACAGACCACCTGAGATACAAGGTTGGATGTTGCATGTGTATGCATGCCCTGTTCATTATTAGTCTTCCCATCTTTTATTTTACGGATTGGAGACATTGTCAGTGACCATCCTTTCCGTGCCATATTTCTCAAATATATGATACTGCCCCTTCAGTATTAAAAATGAGAACAGCATGACCAGATGCTCTGGACCTTGGATATGTTCAAGATCATTAGAGCCATGTAGGGACATCTTTTCTGTTTTGGAATTCAAGGGCACCGAGCTTTTAGCAACCTATGCGTTCTTTGATTTTGTTAAGGATTCCAAAACGTTTTTCCATACTTGATTGATGCATTTCTGTTGGCTTCAGGTAGATAGACTCTAACATCTCTACCCCCCATCTCCCTGGTACAGTTTTTGAATGCTTTTGACAGAGCAATGAACTTGCTTGATGATAAGTTTTCCTTCCTTGCATCAAGTAAGCAGATTGTGAGCTGCACAAGTGAAGAAGACAAGGTGCATTCTCCCTCAGACCCTGAATAGCTAAATATTGTTCACAATAATTTTTATTCTTGGATTATTGGTCAGAAGTCAGAACTATCACAATGATGGAGTCGCGATAAAATGCGAAGTGTGCTATGTTGCCTTAGTATTTGAATGGAATGAAAATAATCTATGATAGTTCGAGGTTCCAATTGGAGATTAATTACCTGAGACGAAAAAGGCCAGCTATGGCCATTACTAGAAGACCATCGATGTGTAGATGAAGCCTGGAGTTGATCATAAACACTGCTTCCCAAACTTTGAAAACCATGTAGTTAACTGACTGTTGTGATCCATGTAGGTGATCGTCTTTGAACGTGGAGACCTAGTGTTTGTGTTCAACTTTCATCCTGTAAACACATACGACGGGTACGTGATTTTGTAACATTAAGCGGTTTGTATCTGCCTGCATATCAATAAGTTCACAAGACAGCATAGCATACTAGTCACATTGTATATGGGTTGAATCACAATCGCTGTATAGCCAATCCAATCTTACAATTATATTATTTTGTATACTCCAAACACATACAGATACAAAGTCGGGTGCGACTTACCAGGAAAGTATAGAGTCGCACTTGATAGCGACGCATTGGACTTTGGCGGACATGGAAGAGTAAGAAATCCTTGGTGCTTTTATCATTTTCTTATGCTAGTGCCCAGAGAATGTAGAAGCCTTCTGAAGTTTTTCTTCATTCCCTTCTTTGGGATGCATAATAAACTGCATGTTGCCTGCCTCTCTGATCTTTAGCTTTTTACTTATTGGTAACAGGTAGGCCACGACGTCGACCATTTTACGTCCCCAGAAGGAATCCCCGGGTTGCCTGAAACAAACTTTAACAACCGACCCAATTCCTTCAAAGTACTCTCACCAGCTCGAACGTGTGTGGTATGATAAACAATGGCGCCTTTCGGTTTCTTTGCATTTTCTTTTCTGCGTTGTCTGTTTGATTGTTTTCTCATTGTATGATCCTTGAAGAAGATCAAGGATCCTTCCATCTCCCTCATGGCATTCAAATGACATTTTTGTGCTTCCCTCGACGAGAGCTCGAATAGATTTTTCATAAATACGTCTGCTATGGTTTTCTTTTAAAGGTTTATTACAGAGTTGATGAAAGCAAAGAAGAAAAAGAAAATGGTGATTCAGTTGGTGCAAACAAGCCGCTTGCCACGGAGGTCTTGGCAAGGCAAGTAGAAGAGGACTACGAGGGATTGACACGCCCCAAAGAGGGTGACATTGTTGCAGGTGAGATTACTAATGTTGAAGACGACACGAGCAAGATTGAAGATGGTGACGTTGATCCAAGCAAGATAGAAGATTTACCAGTGAAAGAGACCAAAGCAAGAACATCAGATGAGTAG

mRNA sequence

ATGCTGGCTTCTCCTTCCGTTCTTCCTCCATCAGCTCTTGCTTCTTTGTCACTTTCGGCAAAGAAAAATATCACTACGGACTCGAGAAATAAATGTCCTTCATTGAGACAACAAATTGAGCTGATTTTTGGGTCTAGAATGTCGCATCACCGGCAGATGAACCCATTTATGCACCCAATCTCAAATTACAAAAGGGTCCACAGTTCTGCAATCTCTTCCATCATGACAGATGATACATCAACATTGACATCTGCTGACGAAAGCATGGAAAATATTGGCATCTTGAGCCATGATCCAGGCTTGGAACCCTTCAAAGATCATTTCAAATATAGAGTAAGCAGATATGCGGAACTACTCAACCTTTTAGAGAAGTATGAAGGTGGTCTTGACGAATTCGCACGAGGATATCTAAAATTTGGATTTAATAGAGAAGAAGGTGGCATTGAGTATCGTGAATGGGCTCCGGCTGCTCAGGAAGCACAAATTGTTGGGGACTTTAATGGATGGGATGGTTCCAAACACCGAATGAAAAAGAATGAATATGGCGTTTGGAGTATTAAACTTCCTGATTGTGGTGACGAACCAGCTATTGCTCACAATTCAAGAGTTAAGTTCCGTTTCAAGCATGGCGATGGAGTTTGGATTGATCGCATCCCTGCTTGGATTAAATATGCCACTGCGGATCCTACAAGATTTGCAGCACCATATGATGGTGTGTATTGGGACCCGCCTCCTTTGGAAAGGTATGAATTTAAGCATCCACGTCCTACAAAGCCCGAAGCCCCACGGGTATATGACCAGTGTAGATCCGGAACTCCTGAGGACCTAAAATATCTTATTGACAAGGCCCATGGCTTGGGTTTACGGGTGTTGATGGATGTTGTTCATAGCCATGCAAGTAACAATGTCACTGATGGCCTTAATGGCTATGATGTCGGTCAAAGTTCCCAAGATTCTTACTTCCACACTGGAGATCGTGGTTACCATAAGCTGTGGGATAGTAGACTCTTCAATTATGCAAATTGGGAAGTTCTGCGTTTCCTTTTATCGAACGTAAGATGGTGGTTGGACGAGTATAAATTTGATGGTTTTCGATTTGATGGAGTAACTTCAATGCTGTATCAACACCATGGAATCAACATGGCTTTCACTGGAAATTATAAAGAGTATTTTAGTGAAGCAACAGACGTTGATGCTGTTGTGTATTTGATGCTAGCCAATAATCTGATTAACAGCATTTTGCCGGATGCAACTGTAATTGCTGAAGATGTTTCGGGCATGCCTGGTCTTGGAAGACCTGTTTCCGAAGGGGGTATTGGTTTTGACTACCGCCTTCAAATGGCCATTCCTGACAAATGGATTGATTACTTGAAGAACAAGAGTGATGAGGAATGGTCCATGGAGGAAATAAGTTGGAACTTGACAAATAGAAGATATTCTGAGAAGTGCGTATCTTATGCTGAGAGTCATGATCAGGCGATTGTTGGAGACAAGACCATTGCATTTTTGCTGATGGATAAAGAGATGTATTCTGGCATGTCTTGTTTGACAGAGGCATCTCCCATAGTTGAAAGAGGGATTGCACTGCACAAGATGATACATTTTATAACCATGGCCTTGGGAGGGGAGGGCTACCTTAATTTCATGGGAAATGAGTTCGCGCATCCTGAGTGGATTGACTTCCCACGAGAAGGCAATGAGTGGAGTTATGACAAGTGCAGGCGACAGTGGAATCTGCTTCACACAGACCACCTGAGATACAAGTTTTTGAATGCTTTTGACAGAGCAATGAACTTGCTTGATGATAAGTTTTCCTTCCTTGCATCAAGTAAGCAGATTGTGAGCTGCACAAGTGAAGAAGACAAGGTGATCGTCTTTGAACGTGGAGACCTAGTGTTTGTGTTCAACTTTCATCCTGTAAACACATACGACGGATACAAAGTCGGGTGCGACTTACCAGGAAAGTATAGAGTCGCACTTGATAGCGACGCATTGGACTTTGGCGGACATGGAAGAGTAGGCCACGACGTCGACCATTTTACGTCCCCAGAAGGAATCCCCGGGTTGCCTGAAACAAACTTTAACAACCGACCCAATTCCTTCAAAGTACTCTCACCAGCTCGAACGTGTGTGGTTTATTACAGAGTTGATGAAAGCAAAGAAGAAAAAGAAAATGGTGATTCAGTTGGTGCAAACAAGCCGCTTGCCACGGAGGTCTTGGCAAGGCAAGTAGAAGAGGACTACGAGGGATTGACACGCCCCAAAGAGGGTGACATTGTTGCAGGTGAGATTACTAATGTTGAAGACGACACGAGCAAGATTGAAGATGGTGACGTTGATCCAAGCAAGATAGAAGATTTACCAGTGAAAGAGACCAAAGCAAGAACATCAGATGAGTAG

Coding sequence (CDS)

ATGCTGGCTTCTCCTTCCGTTCTTCCTCCATCAGCTCTTGCTTCTTTGTCACTTTCGGCAAAGAAAAATATCACTACGGACTCGAGAAATAAATGTCCTTCATTGAGACAACAAATTGAGCTGATTTTTGGGTCTAGAATGTCGCATCACCGGCAGATGAACCCATTTATGCACCCAATCTCAAATTACAAAAGGGTCCACAGTTCTGCAATCTCTTCCATCATGACAGATGATACATCAACATTGACATCTGCTGACGAAAGCATGGAAAATATTGGCATCTTGAGCCATGATCCAGGCTTGGAACCCTTCAAAGATCATTTCAAATATAGAGTAAGCAGATATGCGGAACTACTCAACCTTTTAGAGAAGTATGAAGGTGGTCTTGACGAATTCGCACGAGGATATCTAAAATTTGGATTTAATAGAGAAGAAGGTGGCATTGAGTATCGTGAATGGGCTCCGGCTGCTCAGGAAGCACAAATTGTTGGGGACTTTAATGGATGGGATGGTTCCAAACACCGAATGAAAAAGAATGAATATGGCGTTTGGAGTATTAAACTTCCTGATTGTGGTGACGAACCAGCTATTGCTCACAATTCAAGAGTTAAGTTCCGTTTCAAGCATGGCGATGGAGTTTGGATTGATCGCATCCCTGCTTGGATTAAATATGCCACTGCGGATCCTACAAGATTTGCAGCACCATATGATGGTGTGTATTGGGACCCGCCTCCTTTGGAAAGGTATGAATTTAAGCATCCACGTCCTACAAAGCCCGAAGCCCCACGGGTATATGACCAGTGTAGATCCGGAACTCCTGAGGACCTAAAATATCTTATTGACAAGGCCCATGGCTTGGGTTTACGGGTGTTGATGGATGTTGTTCATAGCCATGCAAGTAACAATGTCACTGATGGCCTTAATGGCTATGATGTCGGTCAAAGTTCCCAAGATTCTTACTTCCACACTGGAGATCGTGGTTACCATAAGCTGTGGGATAGTAGACTCTTCAATTATGCAAATTGGGAAGTTCTGCGTTTCCTTTTATCGAACGTAAGATGGTGGTTGGACGAGTATAAATTTGATGGTTTTCGATTTGATGGAGTAACTTCAATGCTGTATCAACACCATGGAATCAACATGGCTTTCACTGGAAATTATAAAGAGTATTTTAGTGAAGCAACAGACGTTGATGCTGTTGTGTATTTGATGCTAGCCAATAATCTGATTAACAGCATTTTGCCGGATGCAACTGTAATTGCTGAAGATGTTTCGGGCATGCCTGGTCTTGGAAGACCTGTTTCCGAAGGGGGTATTGGTTTTGACTACCGCCTTCAAATGGCCATTCCTGACAAATGGATTGATTACTTGAAGAACAAGAGTGATGAGGAATGGTCCATGGAGGAAATAAGTTGGAACTTGACAAATAGAAGATATTCTGAGAAGTGCGTATCTTATGCTGAGAGTCATGATCAGGCGATTGTTGGAGACAAGACCATTGCATTTTTGCTGATGGATAAAGAGATGTATTCTGGCATGTCTTGTTTGACAGAGGCATCTCCCATAGTTGAAAGAGGGATTGCACTGCACAAGATGATACATTTTATAACCATGGCCTTGGGAGGGGAGGGCTACCTTAATTTCATGGGAAATGAGTTCGCGCATCCTGAGTGGATTGACTTCCCACGAGAAGGCAATGAGTGGAGTTATGACAAGTGCAGGCGACAGTGGAATCTGCTTCACACAGACCACCTGAGATACAAGTTTTTGAATGCTTTTGACAGAGCAATGAACTTGCTTGATGATAAGTTTTCCTTCCTTGCATCAAGTAAGCAGATTGTGAGCTGCACAAGTGAAGAAGACAAGGTGATCGTCTTTGAACGTGGAGACCTAGTGTTTGTGTTCAACTTTCATCCTGTAAACACATACGACGGATACAAAGTCGGGTGCGACTTACCAGGAAAGTATAGAGTCGCACTTGATAGCGACGCATTGGACTTTGGCGGACATGGAAGAGTAGGCCACGACGTCGACCATTTTACGTCCCCAGAAGGAATCCCCGGGTTGCCTGAAACAAACTTTAACAACCGACCCAATTCCTTCAAAGTACTCTCACCAGCTCGAACGTGTGTGGTTTATTACAGAGTTGATGAAAGCAAAGAAGAAAAAGAAAATGGTGATTCAGTTGGTGCAAACAAGCCGCTTGCCACGGAGGTCTTGGCAAGGCAAGTAGAAGAGGACTACGAGGGATTGACACGCCCCAAAGAGGGTGACATTGTTGCAGGTGAGATTACTAATGTTGAAGACGACACGAGCAAGATTGAAGATGGTGACGTTGATCCAAGCAAGATAGAAGATTTACCAGTGAAAGAGACCAAAGCAAGAACATCAGATGAGTAG

Protein sequence

MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPISNYKRVHSSAISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLNLLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNEYGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVYWDPPPLERYEFKHPRPTKPEAPRVYDQCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNNVTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFDGFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEKCVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGGEGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKEEKENGDSVGANKPLATEVLARQVEEDYEGLTRPKEGDIVAGEITNVEDDTSKIEDGDVDPSKIEDLPVKETKARTSDE
Homology
BLAST of Sgr018488 vs. NCBI nr
Match: XP_022153149.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Momordica charantia])

HSP 1 Score: 1440.6 bits (3728), Expect = 0.0e+00
Identity = 705/858 (82.17%), Postives = 743/858 (86.60%), Query Frame = 0

Query: 1   MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPI 60
           MLAS  VLPP++LASLSLSAKKN +  SRNK PSLR+QIELIF SRMSHHR MNPFMHPI
Sbjct: 1   MLASSCVLPPTSLASLSLSAKKNFSAGSRNKWPSLRRQIELIFVSRMSHHRHMNPFMHPI 60

Query: 61  SNYKRVHSSAISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLN 120
           S YKR HSSAIS +MTD+  T+TSADE MEN+GILSHDPGLEPFKDHFKYR+ RYA LL+
Sbjct: 61  STYKRGHSSAISCVMTDNAPTITSADEIMENMGILSHDPGLEPFKDHFKYRIGRYANLLS 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNE 180
           LLEK+EGGLDEFA+GYLKFGFNREEGGI YREWAPAAQEAQI+GDFNGWDGS H+M+KNE
Sbjct: 121 LLEKHEGGLDEFAQGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180

Query: 181 YGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVY 240
           +G+WSIKLPD G +PAI HNSRVKFRFKHG+GVWIDRIPAWI+YAT DPTRFAAPYDGVY
Sbjct: 181 FGIWSIKLPDNGGKPAITHNSRVKFRFKHGNGVWIDRIPAWIRYATVDPTRFAAPYDGVY 240

Query: 241 WDPPPLERYEFKHPRPTKPEAPRVYD---------------------------------- 300
           WDPPPLERYEF+HPRP KPEAPR+Y+                                  
Sbjct: 241 WDPPPLERYEFEHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFILPRIKENNYNTV 300

Query: 301 ------------------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360
                                     RSGTPEDLKYLIDKAHGLGLRVLMDV+HSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVIHSHASNN 360

Query: 361 VTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFD 420
           VTDGLNG+DVGQSSQ SYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EYKFD
Sbjct: 361 VTDGLNGFDVGQSSQGSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYKFD 420

Query: 421 GFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAE 480
           GFRFDGVTSMLY HHGINM FTGNY EYFSEATDVDAVVYLMLANNLI+SILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEK 540
           DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSM EISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600
           C+SYAESHDQAIVGDKT+AFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQAIVGDKTVAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDD 660
           EGYLNFMGNEF HPEWIDFPREGN WSYDKCRRQWNL  TDHLRYKFLNAFDRAMNLLDD
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660

Query: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQI+SCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQILSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKEE 780
           DA DFGGHGR+GHD DHFTSPEGIPG+PETNFNNRPNSFKVLSPARTCVVYYRVDESK E
Sbjct: 721 DAWDFGGHGRIGHDADHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYRVDESK-E 780

Query: 781 KENGDSVGANKPLATEVLARQVEEDYEGLTRPKEGDIVAGE--ITNVED---DTSKIEDG 796
           KENGDSVG NKPL T+V AR  EE+ E LT  +EGDI  GE   TNVED   DTSK EDG
Sbjct: 781 KENGDSVGTNKPLTTDVSARHTEEEDEELTHSEEGDIAVGETRTTNVEDDNIDTSKSEDG 840

BLAST of Sgr018488 vs. NCBI nr
Match: XP_038884508.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Benincasa hispida])

HSP 1 Score: 1385.9 bits (3586), Expect = 0.0e+00
Identity = 682/852 (80.05%), Postives = 729/852 (85.56%), Query Frame = 0

Query: 1   MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPI 60
           ML SP VLP  ALASLSLSAKK  T DSRN+ PSLRQ  EL FGS+MS+HRQMNPFM P 
Sbjct: 1   MLPSPCVLPSPALASLSLSAKKIFTLDSRNQWPSLRQ--ELNFGSKMSNHRQMNPFMLPF 60

Query: 61  SNYKRVHSSAISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLN 120
           S YK+VHS AISS++TDDT  ++S DES ENIGILSHDPGLE FKDHFKYRV RYA+LLN
Sbjct: 61  STYKKVHSPAISSVVTDDTPKVSSPDESTENIGILSHDPGLESFKDHFKYRVGRYADLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNE 180
           LL+K+EGGLDEFARGYLKFGFNREE GI YREWAPAAQEAQI+GDFNGWDG+ HRM+KNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWDGTNHRMEKNE 180

Query: 181 YGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVY 240
           +G+WSIK+ D   +PAI+HNSRVKFRFKHG+GVWIDRIPAWIKYAT DPT+FAAPYDGVY
Sbjct: 181 FGIWSIKIRDLSGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPPLERYEFKHPRPTKPEAPRVYD---------------------------------- 300
           WDPPPLERYEFKHPRP KP+APRVY+                                  
Sbjct: 241 WDPPPLERYEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 ------------------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360
                                     RSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFD 420
           VTDGLNG+DVGQS+QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EY+FD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAE 480
           GFRFDGVTSMLY HHGINMAFTG Y EYFSEATDVDAVVYLMLANNL +SILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMAFTGTYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEK 540
           DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSM EISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600
           C+SYAESHDQ+IVGDKTIAFLLMDKEMYSGMSCLT+ASP+VERGI+LHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTDASPVVERGISLHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDD 660
           EGYLNFMGNEF HPEWIDFPREGN WSYDKCRRQWNL  TDHLRYKFLNAFDRAMN LDD
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNSLDD 660

Query: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKEE 780
           DA DFGGHGRVGHDVDHFTSPEGIPG+ ETNFNNRPNSFKVLSPARTCVVY+RVDESK E
Sbjct: 721 DAWDFGGHGRVGHDVDHFTSPEGIPGVSETNFNNRPNSFKVLSPARTCVVYHRVDESK-E 780

Query: 781 KENGDSVGANKPLATEVLARQVEEDYEGLT-RPKEGDIVAGEITNVEDDTS--KIEDGDV 792
           KEN + VGAN+  AT+V AR VE+D EG     +E DI AGEI+ +EDDT   K EDGDV
Sbjct: 781 KENDNLVGANEARATDVFARHVEDDNEGSAGGEEENDIAAGEISKIEDDTDTRKTEDGDV 840

BLAST of Sgr018488 vs. NCBI nr
Match: XP_008464849.1 (PREDICTED: 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like isoform X1 [Cucumis melo] >ADN33992.1 starch branching enzyme [Cucumis melo subsp. melo])

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 676/860 (78.60%), Postives = 727/860 (84.53%), Query Frame = 0

Query: 1   MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPI 60
           ML SP VLP +A+ASLSLSAKK   +DSRN+ PSLRQQ EL F SRMSHHRQMNPF+ P 
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  SNYKRVHSSAISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLN 120
           S YKRVHS AISS+MTDDTST++S DESMENIGILSHDPGL+PFKDHFKYRV RY +LLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNE 180
           LL+K+EGGLDEFA+GYLKFGFNREE GI YREWAPAAQEAQIVGDFNGW+G+ H M+KNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 YGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVY 240
           +G+WSIK+ D G +PAI+HNSRVKFRFKHG+GVWIDRIPAWIKYAT DPT+FAAPYDGVY
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPPLERYEFKHPRPTKPEAPRVYD---------------------------------- 300
           WDPPPLERYEFKHPRP KP  PRVY+                                  
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 ------------------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360
                                     RSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFD 420
           VTDGLNG+DVGQS+QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EY+FD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAE 480
           GFRFDGVTSMLY HHGI+MAFTGNY EYFSEATDVDAVVYLMLANNL +SILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSM EISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600
           C+SYAESHDQ+IVGDKTIAFLLMDKEMYSGMSCL  ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDD 660
           EGYLNFMGNEF HPEWIDFPREGN WSYDKCRRQWNL  TDHLRYKFLNAFDRAMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDE 660

Query: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVS T EEDKVIVFERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKEE 780
           DA DFGG+GRVGHD+DHFTSPEGIPG+PETNFNNRPNSFKVLSPARTCVVYY+VDESK E
Sbjct: 721 DAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESK-E 780

Query: 781 KENGDSVGANKPLATEVLARQVEEDYEGLT-RPKEGDIVAGEITNVED---DTSKIEDGD 799
           KE  D V +   +  +V AR VEED EGL  R +E DI  G+I+  ED   DTSK E+ D
Sbjct: 781 KEKDDLVAS---VHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDTSKTEEDD 840

BLAST of Sgr018488 vs. NCBI nr
Match: XP_004144236.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic [Cucumis sativus] >KGN47537.1 hypothetical protein Csa_019021 [Cucumis sativus])

HSP 1 Score: 1370.9 bits (3547), Expect = 0.0e+00
Identity = 678/852 (79.58%), Postives = 720/852 (84.51%), Query Frame = 0

Query: 1   MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPI 60
           ML S  VLP +A+ASLSLSAKK I +DSRN+ PSLRQQ EL F SRMSHHRQMNPF+ P 
Sbjct: 1   MLLSSCVLPSTAVASLSLSAKKIIISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFVFPY 60

Query: 61  SNYKRVHSSAISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLN 120
           S YKRVHS AISS+MT+DTST++S DESMENIGILSHDPGL+PFKDHFKYRV RY +LLN
Sbjct: 61  STYKRVHSPAISSVMTEDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNE 180
           LL+K+EGGLDEFARGYLKFGFNREE GI YREWAPAAQEAQIVGDFNGWDG+ H M+KNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180

Query: 181 YGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVY 240
           +G+WSIK+ D G +PAI+HNSRVKFRFKHG+GVWIDRIPAWIKYAT DPT+FAAPYDGVY
Sbjct: 181 FGIWSIKVYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPPLERYEFKHPRPTKPEAPRVYD---------------------------------- 300
           WDPPPLERYEFKHPRP KP APRVY+                                  
Sbjct: 241 WDPPPLERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNTV 300

Query: 301 ------------------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360
                                     RSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFD 420
           VTDGLNG+DVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EY+FD
Sbjct: 361 VTDGLNGFDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAE 480
           GFRFDGVTSMLY HHGINM F+GNY EYFSEATDVDAVVYLMLANNL +SILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFSGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSM EISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600
           C+SYAESHDQ+IVGDKTIAFLLMDKEMYSGMSCL  ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDD 660
           EGYLNFMGNEF HPEWIDFPREGN WSYDKCRRQWNL  TDHLRYKFLNAFD AMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDSAMNALDE 660

Query: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVS T EEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKEE 780
           DA DFGGHGRVGHD+DHFTSPEGIPG+PETNFNNRPNSFK+LSPARTCVVYY+VDESK E
Sbjct: 721 DASDFGGHGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKILSPARTCVVYYKVDESK-E 780

Query: 781 KENGDSVGANKPLATEVLARQVEEDYEGLTRPK-EGDIVAGEITNVED---DTSKIEDGD 791
           KE  D VG+      +V AR VEED EGL   K E DI  GEI+  ED   DTSK ED D
Sbjct: 781 KEKDDLVGSAN---EDVFARHVEEDSEGLAGCKEENDIAVGEISKTEDDDIDTSKPEDDD 840

BLAST of Sgr018488 vs. NCBI nr
Match: KAG6599100.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1347.8 bits (3487), Expect = 0.0e+00
Identity = 664/851 (78.03%), Postives = 716/851 (84.14%), Query Frame = 0

Query: 1   MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPI 60
           MLASP VLP +ALASLSLSAKKN T+DSRN+C SLRQQ  L FGSRMSHHRQM+PFM P 
Sbjct: 1   MLASPCVLPSTALASLSLSAKKNFTSDSRNQCSSLRQQFVLNFGSRMSHHRQMHPFMAPF 60

Query: 61  SNYKRVHSSA-ISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELL 120
           S YKRVHS A ISS+MTDDTST++SADE+M++IGIL HDP LEPFKDHFKYR+ RY+++L
Sbjct: 61  STYKRVHSPAIISSVMTDDTSTVSSADENMKDIGILRHDPSLEPFKDHFKYRIGRYSDVL 120

Query: 121 NLLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKN 180
           NL +K+EGGLDEFARGYLKFGFNRE  GI YREWAP AQEAQI+GDFNGWDGS HRM+KN
Sbjct: 121 NLFDKHEGGLDEFARGYLKFGFNREGDGIVYREWAPVAQEAQIIGDFNGWDGSNHRMEKN 180

Query: 181 EYGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGV 240
           E+G+WSI +PD   +PAIAHNSRVKFRFKHG+G WIDRIPAWIKYATADPTRF APYDGV
Sbjct: 181 EFGIWSINIPDIDGKPAIAHNSRVKFRFKHGNGAWIDRIPAWIKYATADPTRFGAPYDGV 240

Query: 241 YWDPPPLERYEFKHPRPTKPEAPRVYD--------------------------------- 300
           YW+PPPLERYEFKHPRP KPEAPR+Y+                                 
Sbjct: 241 YWEPPPLERYEFKHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNT 300

Query: 301 -------------------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASN 360
                                      RSGTPEDLKYLIDKAHGLGLRVLMD+VHSHASN
Sbjct: 301 VQLMAIMEHSYYASFGYHVTNFFAISSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASN 360

Query: 361 NVTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKF 420
           NVTDGLNG+DVGQS+QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EY+F
Sbjct: 361 NVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQF 420

Query: 421 DGFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIA 480
           DGFRFDGVTSMLY HHGIN AFTGNY EYFSEATDVDAVVYLMLANNLI+SILP+ATVIA
Sbjct: 421 DGFRFDGVTSMLYHHHGINTAFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPNATVIA 480

Query: 481 EDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSE 540
           EDVSGMPGLG PVSEGGIGFDYRLQMAIPDKWIDY+KNK+DEEWSM EISWNLTNRRYSE
Sbjct: 481 EDVSGMPGLGIPVSEGGIGFDYRLQMAIPDKWIDYVKNKNDEEWSMGEISWNLTNRRYSE 540

Query: 541 KCVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALG 600
           KC+SYAESHDQ+IVGDKTIAFLLMDKEMYSGMSCLTEASP+VERGIALHKMIHFITMALG
Sbjct: 541 KCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTEASPVVERGIALHKMIHFITMALG 600

Query: 601 GEGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLD 660
           GEGYLNFMGNEF HPEWIDFPREGN WSYDKCRRQWNL  TDHLRYKFLNAFDRAMN LD
Sbjct: 601 GEGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNSLD 660

Query: 661 DKFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALD 720
           DKFSFLASSKQIVSCTSEE+KVIVFERGDLVFVFNFHP+NTYDGYKVGCD+PGKYRVALD
Sbjct: 661 DKFSFLASSKQIVSCTSEEEKVIVFERGDLVFVFNFHPINTYDGYKVGCDIPGKYRVALD 720

Query: 721 SDALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKE 780
           SDA +FGG GRVGHD+DHFTSPEGIPG+PETNFNNRPNSFKVLSPA+TCVVYYRV ESK 
Sbjct: 721 SDASEFGGRGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPAQTCVVYYRVYESK- 780

Query: 781 EKENGDSVGANKPLATEVLARQVEEDYEGLTRPKEGDIVAGEITNVEDDTSKIE-DGDVD 792
           EKENG SVGA++  AT+V+          L R KEG+I            SKIE D DV 
Sbjct: 781 EKENGVSVGASEAHATDVV----------LARDKEGEI------------SKIEGDDDVH 828

BLAST of Sgr018488 vs. ExPASy Swiss-Prot
Match: Q41059 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic (Fragment) OS=Pisum sativum OX=3888 GN=SBEII PE=1 SV=1)

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 549/763 (71.95%), Postives = 608/763 (79.69%), Query Frame = 0

Query: 25  TTDSRNKCPSLRQQ-IELIFGSRMSHHRQMNPFMHPISNYKRVHSSAIS-SIMTDDTSTL 84
           T +S+NK    +Q+ +EL  G +  +  ++  F    S Y++V S     S+MTDD ST+
Sbjct: 7   THNSKNKQYLAKQKPVELTLGYQNPNGCKVCSFGSKGSIYQKVSSGFKGVSVMTDDKSTM 66

Query: 85  TSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLNLLEKYEGGLDEFARGYLKFGFN 144
            S +E  ENIGIL+ D  LEPFKDHFKYR+ RY     L+E+YEGGL EFA+GYLKFGFN
Sbjct: 67  PSVEEDFENIGILNVDSSLEPFKDHFKYRLKRYLHQKKLIEEYEGGLQEFAKGYLKFGFN 126

Query: 145 REEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNEYGVWSIKLPDCGDEPAIAHNSR 204
           REE GI YREWAPAAQEAQI+GDFNGW+GS   M+K+++GVWSI++PD    PAI HNSR
Sbjct: 127 REEDGISYREWAPAAQEAQIIGDFNGWNGSNLHMEKDQFGVWSIQIPDADGNPAIPHNSR 186

Query: 205 VKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVYWDPPPLERYEFKHPRPTKPEAP 264
           VKFRFKH DGVW+DRIPAWIKYAT DPTRFAAPYDGVYWDPP  ERY+FKHPRP KP+AP
Sbjct: 187 VKFRFKHSDGVWVDRIPAWIKYATVDPTRFAAPYDGVYWDPPLSERYQFKHPRPPKPKAP 246

Query: 265 RVYD-------------------------------------------------------- 324
           R+Y+                                                        
Sbjct: 247 RIYEAHVGMSSSEPRINSYREFADDVLPRIRENNYNTVQLMAVMEHSYYASFWYHVTKPF 306

Query: 325 ---QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNNVTDGLNGYDVGQSSQDSYFHT 384
                RSG+PEDLKYLIDKAH LGL VLMDV+HSHASNNVTDGLNG+DVGQSSQ SYFH 
Sbjct: 307 FAVSSRSGSPEDLKYLIDKAHSLGLNVLMDVIHSHASNNVTDGLNGFDVGQSSQQSYFHA 366

Query: 385 GDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFDGFRFDGVTSMLYQHHGINMAF 444
           GDRGYHKLWDSRLFNYANW+   FLLSN+RWWL+EYKFDGFRFDGVTSMLY HHGINMAF
Sbjct: 367 GDRGYHKLWDSRLFNYANWK-SSFLLSNLRWWLEEYKFDGFRFDGVTSMLYHHHGINMAF 426

Query: 445 TGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAEDVSGMPGLGRPVSEGGIGFDY 504
           TG+Y EYFSE TDVDAVVYLMLAN+L++ ILPDAT IAEDVSGMPGLGRPVSE GIGFDY
Sbjct: 427 TGDYNEYFSEETDVDAVVYLMLANSLVHDILPDATDIAEDVSGMPGLGRPVSEVGIGFDY 486

Query: 505 RLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEKCVSYAESHDQAIVGDKTIAFL 564
           RL MAIPDKWIDYLKNK D EWSM+EIS NLTNRRY+EKCVSYAESHDQ+IVGDKTIAFL
Sbjct: 487 RLAMAIPDKWIDYLKNKKDSEWSMKEISLNLTNRRYTEKCVSYAESHDQSIVGDKTIAFL 546

Query: 565 LMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGGEGYLNFMGNEFAHPEWIDFPR 624
           LMD+EMYS MSCLT  SP +ERGI+LHKMIHFIT+ALGGEGYLNFMGNEF HPEWIDFPR
Sbjct: 547 LMDEEMYSSMSCLTMLSPTIERGISLHKMIHFITLALGGEGYLNFMGNEFGHPEWIDFPR 606

Query: 625 EGNEWSYDKCR-RQWNLLHTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSCTSEEDK 684
           EGN WSY+KCR  QWNL+ T+HLRYKF+NAFDRAMNLLDDKFS LAS+KQIVS T+ EDK
Sbjct: 607 EGNGWSYEKCRLTQWNLVDTNHLRYKFMNAFDRAMNLLDDKFSILASTKQIVSSTNNEDK 666

Query: 685 VIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHDVDHFTS 726
           VIVFERGDLVFVFNFHP NTY+GYKVGCDLPGKYRVALDSDA +FGGHGRVGHD D FTS
Sbjct: 667 VIVFERGDLVFVFNFHPENTYEGYKVGCDLPGKYRVALDSDATEFGGHGRVGHDADQFTS 726

BLAST of Sgr018488 vs. ExPASy Swiss-Prot
Match: P30924 (1,4-alpha-glucan-branching enzyme OS=Solanum tuberosum OX=4113 GN=SBE1 PE=2 SV=2)

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 516/732 (70.49%), Postives = 588/732 (80.33%), Query Frame = 0

Query: 28  SRNK-CPSLRQQIELIFGSR-MSHHRQMNPFMHPISNYKRVHSSAISSIMTDDTSTLTSA 87
           SRNK C   +    L FGS+  S      P      + +  HSSAIS+++TDD ST+   
Sbjct: 28  SRNKICFPSQHSTGLKFGSQERSWDISSTPKSRVRKDERMKHSSAISAVLTDDNSTMAPL 87

Query: 88  DESM--ENIGILSHDPGLEPFKDHFKYRVSRYAELLNLLEKYEGGLDEFARGYLKFGFNR 147
           +E +  ENIG+L+ DP LEP+ DHF++R+ RY +   L+EKYEG L+EFA+GYLKFGFNR
Sbjct: 88  EEDVKTENIGLLNLDPTLEPYLDHFRHRMKRYVDQKMLIEKYEGPLEEFAQGYLKFGFNR 147

Query: 148 EEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNEYGVWSIKLPDCGDEPAIAHNSRV 207
           E+G I YREWAPAAQE +++GDFNGW+GS H M+K+++GVWSI++PD   +P I HNSRV
Sbjct: 148 EDGCIVYREWAPAAQEDEVIGDFNGWNGSNHMMEKDQFGVWSIRIPDVDSKPVIPHNSRV 207

Query: 208 KFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVYWDPPPLERYEFKHPRPTKPEAPR 267
           KFRFKHG+GVW+DRIPAWIKYATAD T+FAAPYDGVYWDPPP ERY FK+PRP KP APR
Sbjct: 208 KFRFKHGNGVWVDRIPAWIKYATADATKFAAPYDGVYWDPPPSERYHFKYPRPPKPRAPR 267

Query: 268 VYD--------------------------------------------------------- 327
           +Y+                                                         
Sbjct: 268 IYEAHVGMSSSEPRVNSYREFADDVLPRIKANNYNTVQLMAIMEHSYYGSFGYHVTNFFA 327

Query: 328 -QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNNVTDGLNGYDVGQSSQDSYFHTGD 387
              R G PEDLKYLIDKAH LGL+VL+DVVHSHASNNVTDGLNG+D+GQ SQ+SYFH G+
Sbjct: 328 VSSRYGNPEDLKYLIDKAHSLGLQVLVDVVHSHASNNVTDGLNGFDIGQGSQESYFHAGE 387

Query: 388 RGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFDGFRFDGVTSMLYQHHGINMAFTG 447
           RGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EY FDGFRFDG+TSMLY HHGINM FTG
Sbjct: 388 RGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYNFDGFRFDGITSMLYVHHGINMGFTG 447

Query: 448 NYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAEDVSGMPGLGRPVSEGGIGFDYRL 507
           NY EYFSEATDVDAVVYLMLANNLI+ I PDATVIAEDVSGMPGLGRPVSEGGIGFDYRL
Sbjct: 448 NYNEYFSEATDVDAVVYLMLANNLIHKIFPDATVIAEDVSGMPGLGRPVSEGGIGFDYRL 507

Query: 508 QMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEKCVSYAESHDQAIVGDKTIAFLLM 567
            MAIPDKWIDYLKNK+DE+WSM+E++ +LTNRRY+EKC++YAESHDQ+IVGDKTIAFLLM
Sbjct: 508 AMAIPDKWIDYLKNKNDEDWSMKEVTSSLTNRRYTEKCIAYAESHDQSIVGDKTIAFLLM 567

Query: 568 DKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGGEGYLNFMGNEFAHPEWIDFPREG 627
           DKEMYSGMSCLT+ASP+V+RGIALHKMIHF TMALGGEGYLNFMGNEF HPEWIDFPREG
Sbjct: 568 DKEMYSGMSCLTDASPVVDRGIALHKMIHFFTMALGGEGYLNFMGNEFGHPEWIDFPREG 627

Query: 628 NEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSCTSEEDKVIV 687
           N WSYDKCRRQWNL  ++HLRYKF+NAFDRAMN LD+KFSFLAS KQIVS   +++KV+V
Sbjct: 628 NNWSYDKCRRQWNLADSEHLRYKFMNAFDRAMNSLDEKFSFLASGKQIVSSMDDDNKVVV 687

Query: 688 FERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHDVDHFTSPEG 698
           FERGDLVFVFNFHP NTY+GYKVGCDLPGKYRVALDSDA +FGGHGR GHDVDHFTSPEG
Sbjct: 688 FERGDLVFVFNFHPKNTYEGYKVGCDLPGKYRVALDSDAWEFGGHGRTGHDVDHFTSPEG 747

BLAST of Sgr018488 vs. ExPASy Swiss-Prot
Match: Q01401 (1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SBE1 PE=1 SV=2)

HSP 1 Score: 1052.7 bits (2721), Expect = 2.0e-306
Identity = 490/718 (68.25%), Postives = 573/718 (79.81%), Query Frame = 0

Query: 81  TLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLNLLEKYEGGLDEFARGYLKFG 140
           T+ +  E ++++ I   DP LE FKDHF YR+ RY +   L+EK+EGGL+EF++GYLKFG
Sbjct: 65  TMVTVVEEVDHLPIYDLDPKLEEFKDHFNYRIKRYLDQKCLIEKHEGGLEEFSKGYLKFG 124

Query: 141 FNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNEYGVWSIKLPDCGDEPAIAHN 200
            N  +G   YREWAPAAQEAQ++G+FN W+G+KH+M+K+++G+WSIK+     +PAI HN
Sbjct: 125 INTVDGATIYREWAPAAQEAQLIGEFNNWNGAKHKMEKDKFGIWSIKISHVNGKPAIPHN 184

Query: 201 SRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVYWDPPPLERYEFKHPRPTKPE 260
           S+VKFRF+HG G W+DRIPAWI+YAT D ++F APYDGV+WDPP  ERY FKHPRP KP+
Sbjct: 185 SKVKFRFRHGGGAWVDRIPAWIRYATFDASKFGAPYDGVHWDPPACERYVFKHPRPPKPD 244

Query: 261 APRVYD------------------------------------------------------ 320
           APR+Y+                                                      
Sbjct: 245 APRIYEAHVGMSGEEPEVSTYREFADNVLPRIRANNYNTVQLMAIMEHSYYASFGYHVTN 304

Query: 321 ----QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNNVTDGLNGYDVGQSSQDSYFH 380
                 RSGTPEDLKYL+DKAH LGLRVLMDVVHSHASNNVTDGLNGYDVGQ++ +SYFH
Sbjct: 305 FFAVSSRSGTPEDLKYLVDKAHSLGLRVLMDVVHSHASNNVTDGLNGYDVGQNTHESYFH 364

Query: 381 TGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFDGFRFDGVTSMLYQHHGINMA 440
           TGDRGYHKLWDSRLFNYANWEVLRFLLSN+R+W+DE+ FDGFRFDGVTSMLY HHGIN  
Sbjct: 365 TGDRGYHKLWDSRLFNYANWEVLRFLLSNLRYWMDEFMFDGFRFDGVTSMLYHHHGINKG 424

Query: 441 FTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAEDVSGMPGLGRPVSEGGIGFD 500
           FTGNYKEYFS  TDVDA+VY+MLAN+L++ +LP+AT++AEDVSGMP L RPV EGG+GFD
Sbjct: 425 FTGNYKEYFSLDTDVDAIVYMMLANHLMHKLLPEATIVAEDVSGMPVLCRPVDEGGVGFD 484

Query: 501 YRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEKCVSYAESHDQAIVGDKTIAF 560
           +RL MAIPD+WIDYLKNK D +WSM EI   LTNRRY+EKC++YAESHDQ+IVGDKTIAF
Sbjct: 485 FRLAMAIPDRWIDYLKNKEDRKWSMSEIVQTLTNRRYTEKCIAYAESHDQSIVGDKTIAF 544

Query: 561 LLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGGEGYLNFMGNEFAHPEWIDFP 620
           LLMDKEMY+GMS L  ASP + RGIAL KMIHFITMALGG+GYLNFMGNEF HPEWIDFP
Sbjct: 545 LLMDKEMYTGMSDLQPASPTINRGIALQKMIHFITMALGGDGYLNFMGNEFGHPEWIDFP 604

Query: 621 REGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSCTSEEDK 680
           REGN WSYDKCRRQW+L+ TDHLRYK++NAFD+AMN L+++FSFL+SSKQIVS  +E+DK
Sbjct: 605 REGNNWSYDKCRRQWSLVDTDHLRYKYMNAFDQAMNALEEEFSFLSSSKQIVSDMNEKDK 664

Query: 681 VIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHDVDHFTS 740
           VIVFERGDLVFVFNFHP  TY GYKVGCDLPGKYRVALDSDAL FGGHGRVGHDVDHFTS
Sbjct: 665 VIVFERGDLVFVFNFHPNKTYKGYKVGCDLPGKYRVALDSDALVFGGHGRVGHDVDHFTS 724

BLAST of Sgr018488 vs. ExPASy Swiss-Prot
Match: Q08047 (1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic OS=Zea mays OX=4577 GN=SBE1 PE=1 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 3.7e-207
Identity = 368/694 (53.03%), Postives = 451/694 (64.99%), Query Frame = 0

Query: 94  ILSHDPGLEPFKDHFKYRVSRYAELLNLLEKYEGGLDEFARGYLKFGFNREEGGIEYREW 153
           I   DP L+ +K H +YR S Y  + + ++++EGGL+ F+R Y KFGFN    GI YREW
Sbjct: 121 IFQIDPMLQGYKYHLEYRYSLYRRIRSDIDEHEGGLEAFSRSYEKFGFNASAEGITYREW 180

Query: 154 APAAQEAQIVGDFNGWDGSKHRMKKNEYGVWSIKLPDCGDEPA-IAHNSRVKFRFKHGDG 213
           AP A  A +VGD N WD +  RM KNE+GVW I LP+  D  + I H SRVK R     G
Sbjct: 181 APGAFSAALVGDVNNWDPNADRMSKNEFGVWEIFLPNNADGTSPIPHGSRVKVRMDTPSG 240

Query: 214 VWIDRIPAWIKYATADPTRFAAPYDGVYWDPPPLERYEFKHPRPTKPEAPRVYD------ 273
           +  D IPAWIKY+   P     PYDG+Y+DPP   +Y F+H +P +P++ R+Y+      
Sbjct: 241 I-KDSIPAWIKYSVQAPGEI--PYDGIYYDPPEEVKYVFRHAQPKRPKSLRIYETHVGMS 300

Query: 274 ----------------------------------------------------QCRSGTPE 333
                                                                 R GTPE
Sbjct: 301 SPEPKINTYVNFRDEVLPRIKKLGYNAVQIMAIQEHSYYGSFGYHVTNFFAPSSRFGTPE 360

Query: 334 DLKYLIDKAHGLGLRVLMDVVHSHASNNVTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDS 393
           DLK LID+AH LGL VLMDVVHSHAS+N  DGLNG+D    +   YFH+G RG+H +WDS
Sbjct: 361 DLKSLIDRAHELGLLVLMDVVHSHASSNTLDGLNGFD---GTDTHYFHSGPRGHHWMWDS 420

Query: 394 RLFNYANWEVLRFLLSNVRWWLDEYKFDGFRFDGVTSMLYQHHGINMAFTGNYKEYFSEA 453
           RLFNY NWEVLRFLLSN RWWL+EYKFDGFRFDGVTSM+Y HHG+ + FTGN+ EYF  A
Sbjct: 421 RLFNYGNWEVLRFLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVTFTGNFNEYFGFA 480

Query: 454 TDVDAVVYLMLANNLINSILPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWI 513
           TDVDAVVYLML N+LI+ + P+A  I EDVSGMP    PV +GG+GFDYR+ MA+ DKWI
Sbjct: 481 TDVDAVVYLMLVNDLIHGLYPEAVTIGEDVSGMPTFALPVHDGGVGFDYRMHMAVADKWI 540

Query: 514 DYLKNKSDEEWSMEEISWNLTNRRYSEKCVSYAESHDQAIVGDKTIAFLLMDKEMYSGMS 573
           D LK +SDE W M +I   LTNRR+ EKCV+YAESHDQA+VGDKTIAF LMDK+MY  M+
Sbjct: 541 DLLK-QSDETWKMGDIVHTLTNRRWLEKCVTYAESHDQALVGDKTIAFWLMDKDMYDFMA 600

Query: 574 CLTEASPIVERGIALHKMIHFITMALGGEGYLNFMGNEFAHPEWIDFPR----------- 633
               ++P ++RGIALHKMI  ITM LGGEGYLNFMGNEF HPEWIDFPR           
Sbjct: 601 LDRPSTPTIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRGPQRLPSGKFI 660

Query: 634 EGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSCTSEEDKV 693
            GN  SYDKCRR+++L   D+LRY  +  FD+AM  L+ K+ F+ S  Q +S   EEDKV
Sbjct: 661 PGNNNSYDKCRRRFDLGDADYLRYHGMQEFDQAMQHLEQKYEFMTSDHQYISRKHEEDKV 720

Query: 694 IVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHDVDHFTSP 718
           IVFE+GDLVFVFNFH  N+Y  Y++GC  PG Y+V LDSDA  FGG  R+ H  +HFT+ 
Sbjct: 721 IVFEKGDLVFVFNFHCNNSYFDYRIGCRKPGVYKVVLDSDAGLFGGFSRIHHAAEHFTA- 780

BLAST of Sgr018488 vs. ExPASy Swiss-Prot
Match: O23647 (1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SBE2.1 PE=1 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 4.5e-205
Identity = 360/698 (51.58%), Postives = 450/698 (64.47%), Query Frame = 0

Query: 98  DPGLEPFKDHFKYRVSRYAELLNLLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAA 157
           DP L   ++H  YR  +Y +L   ++K EGGL+ F+RGY  FGF R   GI YREWAP A
Sbjct: 164 DPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTRSATGITYREWAPGA 223

Query: 158 QEAQIVGDFNGWDGSKHRMKKNEYGVWSIKLPDCGD-EPAIAHNSRVKFRFKHGDGVWID 217
           + A ++GDFN W+     M +N++GVW I LP+  D  PAI H SRVK R     G+  D
Sbjct: 224 KAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGSRVKIRMDTPSGI-KD 283

Query: 218 RIPAWIKYATADPTRFAAPYDGVYWDPPPLERYEFKHPRPTKPEAPRVYD---------- 277
            IPAWIKY+   P     PY+GVY+DPP  ++Y FKHPRP KP + R+Y+          
Sbjct: 284 SIPAWIKYSVQPPGEI--PYNGVYYDPPEEDKYAFKHPRPKKPTSLRIYESHVGMSSTEP 343

Query: 278 ------------------------------------------------QCRSGTPEDLKY 337
                                                             R GTP+DLK 
Sbjct: 344 KINTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKS 403

Query: 338 LIDKAHGLGLRVLMDVVHSHASNNVTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFN 397
           LIDKAH LGL VLMD+VHSHAS N  DGL+ +D    +   YFH+G RGYH +WDSRLFN
Sbjct: 404 LIDKAHELGLVVLMDIVHSHASKNTLDGLDMFD---GTDGQYFHSGSRGYHWMWDSRLFN 463

Query: 398 YANWEVLRFLLSNVRWWLDEYKFDGFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVD 457
           Y +WEVLR+LLSN RWWL+EYKFDGFRFDGVTSM+Y HHG+ + FTGNY EYF  +TDVD
Sbjct: 464 YGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYSTDVD 523

Query: 458 AVVYLMLANNLINSILPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLK 517
           AVVYLML N+LI+ + P+A V+ EDVSGMP    PV +GG+GFDYRL MA+ DKWI+ LK
Sbjct: 524 AVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVGFDYRLHMAVADKWIELLK 583

Query: 518 NKSDEEWSMEEISWNLTNRRYSEKCVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTE 577
            K DE+W + +I++ LTNRR+ EKCV YAESHDQA+VGDKTIAF LMDK+MY  M+   +
Sbjct: 584 -KRDEDWQVGDITFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRQ 643

Query: 578 ASPIVERGIALHKMIHFITMALGGEGYLNFMGNEFAHPEWIDFPR-----------EGNE 637
           A+P V+RGIALHKMI  ITM LGGEGYLNFMGNEF HPEWIDFPR            GN 
Sbjct: 644 ATPRVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRTDQHLPDGRVIAGNN 703

Query: 638 WSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSCTSEEDKVIVFE 697
            SYDKCRR+++L   ++LRY  L  FDRAM  L++ + F+ S  Q +S   E D+VIVFE
Sbjct: 704 GSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMTSEHQYISRKDEGDRVIVFE 763

Query: 698 RGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHDVDHFTSPEGIP 726
           RG+L+FVFNFH  N+Y  Y++GC +PGKY++ LDSD   FGG  R+    + FTS     
Sbjct: 764 RGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFGGFNRLDDSAEFFTS----- 823

BLAST of Sgr018488 vs. ExPASy TrEMBL
Match: A0A6J1DI71 (1,4-alpha-glucan branching enzyme OS=Momordica charantia OX=3673 GN=LOC111020717 PE=3 SV=1)

HSP 1 Score: 1440.6 bits (3728), Expect = 0.0e+00
Identity = 705/858 (82.17%), Postives = 743/858 (86.60%), Query Frame = 0

Query: 1   MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPI 60
           MLAS  VLPP++LASLSLSAKKN +  SRNK PSLR+QIELIF SRMSHHR MNPFMHPI
Sbjct: 1   MLASSCVLPPTSLASLSLSAKKNFSAGSRNKWPSLRRQIELIFVSRMSHHRHMNPFMHPI 60

Query: 61  SNYKRVHSSAISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLN 120
           S YKR HSSAIS +MTD+  T+TSADE MEN+GILSHDPGLEPFKDHFKYR+ RYA LL+
Sbjct: 61  STYKRGHSSAISCVMTDNAPTITSADEIMENMGILSHDPGLEPFKDHFKYRIGRYANLLS 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNE 180
           LLEK+EGGLDEFA+GYLKFGFNREEGGI YREWAPAAQEAQI+GDFNGWDGS H+M+KNE
Sbjct: 121 LLEKHEGGLDEFAQGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180

Query: 181 YGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVY 240
           +G+WSIKLPD G +PAI HNSRVKFRFKHG+GVWIDRIPAWI+YAT DPTRFAAPYDGVY
Sbjct: 181 FGIWSIKLPDNGGKPAITHNSRVKFRFKHGNGVWIDRIPAWIRYATVDPTRFAAPYDGVY 240

Query: 241 WDPPPLERYEFKHPRPTKPEAPRVYD---------------------------------- 300
           WDPPPLERYEF+HPRP KPEAPR+Y+                                  
Sbjct: 241 WDPPPLERYEFEHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFILPRIKENNYNTV 300

Query: 301 ------------------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360
                                     RSGTPEDLKYLIDKAHGLGLRVLMDV+HSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVIHSHASNN 360

Query: 361 VTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFD 420
           VTDGLNG+DVGQSSQ SYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EYKFD
Sbjct: 361 VTDGLNGFDVGQSSQGSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYKFD 420

Query: 421 GFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAE 480
           GFRFDGVTSMLY HHGINM FTGNY EYFSEATDVDAVVYLMLANNLI+SILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEK 540
           DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSM EISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600
           C+SYAESHDQAIVGDKT+AFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQAIVGDKTVAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDD 660
           EGYLNFMGNEF HPEWIDFPREGN WSYDKCRRQWNL  TDHLRYKFLNAFDRAMNLLDD
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660

Query: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQI+SCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQILSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKEE 780
           DA DFGGHGR+GHD DHFTSPEGIPG+PETNFNNRPNSFKVLSPARTCVVYYRVDESK E
Sbjct: 721 DAWDFGGHGRIGHDADHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYRVDESK-E 780

Query: 781 KENGDSVGANKPLATEVLARQVEEDYEGLTRPKEGDIVAGE--ITNVED---DTSKIEDG 796
           KENGDSVG NKPL T+V AR  EE+ E LT  +EGDI  GE   TNVED   DTSK EDG
Sbjct: 781 KENGDSVGTNKPLTTDVSARHTEEEDEELTHSEEGDIAVGETRTTNVEDDNIDTSKSEDG 840

BLAST of Sgr018488 vs. ExPASy TrEMBL
Match: E5GBZ3 (1,4-alpha-glucan branching enzyme OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 676/860 (78.60%), Postives = 727/860 (84.53%), Query Frame = 0

Query: 1   MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPI 60
           ML SP VLP +A+ASLSLSAKK   +DSRN+ PSLRQQ EL F SRMSHHRQMNPF+ P 
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  SNYKRVHSSAISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLN 120
           S YKRVHS AISS+MTDDTST++S DESMENIGILSHDPGL+PFKDHFKYRV RY +LLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNE 180
           LL+K+EGGLDEFA+GYLKFGFNREE GI YREWAPAAQEAQIVGDFNGW+G+ H M+KNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 YGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVY 240
           +G+WSIK+ D G +PAI+HNSRVKFRFKHG+GVWIDRIPAWIKYAT DPT+FAAPYDGVY
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPPLERYEFKHPRPTKPEAPRVYD---------------------------------- 300
           WDPPPLERYEFKHPRP KP  PRVY+                                  
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 ------------------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360
                                     RSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFD 420
           VTDGLNG+DVGQS+QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EY+FD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAE 480
           GFRFDGVTSMLY HHGI+MAFTGNY EYFSEATDVDAVVYLMLANNL +SILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSM EISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600
           C+SYAESHDQ+IVGDKTIAFLLMDKEMYSGMSCL  ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDD 660
           EGYLNFMGNEF HPEWIDFPREGN WSYDKCRRQWNL  TDHLRYKFLNAFDRAMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDE 660

Query: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVS T EEDKVIVFERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKEE 780
           DA DFGG+GRVGHD+DHFTSPEGIPG+PETNFNNRPNSFKVLSPARTCVVYY+VDESK E
Sbjct: 721 DAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESK-E 780

Query: 781 KENGDSVGANKPLATEVLARQVEEDYEGLT-RPKEGDIVAGEITNVED---DTSKIEDGD 799
           KE  D V +   +  +V AR VEED EGL  R +E DI  G+I+  ED   DTSK E+ D
Sbjct: 781 KEKDDLVAS---VHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDTSKTEEDD 840

BLAST of Sgr018488 vs. ExPASy TrEMBL
Match: A0A1S3CMX9 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103502624 PE=3 SV=1)

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 676/860 (78.60%), Postives = 727/860 (84.53%), Query Frame = 0

Query: 1   MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPI 60
           ML SP VLP +A+ASLSLSAKK   +DSRN+ PSLRQQ EL F SRMSHHRQMNPF+ P 
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  SNYKRVHSSAISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLN 120
           S YKRVHS AISS+MTDDTST++S DESMENIGILSHDPGL+PFKDHFKYRV RY +LLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNE 180
           LL+K+EGGLDEFA+GYLKFGFNREE GI YREWAPAAQEAQIVGDFNGW+G+ H M+KNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 YGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVY 240
           +G+WSIK+ D G +PAI+HNSRVKFRFKHG+GVWIDRIPAWIKYAT DPT+FAAPYDGVY
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPPLERYEFKHPRPTKPEAPRVYD---------------------------------- 300
           WDPPPLERYEFKHPRP KP  PRVY+                                  
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 ------------------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360
                                     RSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFD 420
           VTDGLNG+DVGQS+QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EY+FD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAE 480
           GFRFDGVTSMLY HHGI+MAFTGNY EYFSEATDVDAVVYLMLANNL +SILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSM EISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600
           C+SYAESHDQ+IVGDKTIAFLLMDKEMYSGMSCL  ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDD 660
           EGYLNFMGNEF HPEWIDFPREGN WSYDKCRRQWNL  TDHLRYKFLNAFDRAMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDE 660

Query: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVS T EEDKVIVFERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKEE 780
           DA DFGG+GRVGHD+DHFTSPEGIPG+PETNFNNRPNSFKVLSPARTCVVYY+VDESK E
Sbjct: 721 DAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESK-E 780

Query: 781 KENGDSVGANKPLATEVLARQVEEDYEGLT-RPKEGDIVAGEITNVED---DTSKIEDGD 799
           KE  D V +   +  +V AR VEED EGL  R +E DI  G+I+  ED   DTSK E+ D
Sbjct: 781 KEKDDLVAS---VHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDTSKTEEDD 840

BLAST of Sgr018488 vs. ExPASy TrEMBL
Match: A0A0A0KGQ9 (1,4-alpha-glucan branching enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G357030 PE=3 SV=1)

HSP 1 Score: 1370.9 bits (3547), Expect = 0.0e+00
Identity = 678/852 (79.58%), Postives = 720/852 (84.51%), Query Frame = 0

Query: 1   MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPI 60
           ML S  VLP +A+ASLSLSAKK I +DSRN+ PSLRQQ EL F SRMSHHRQMNPF+ P 
Sbjct: 1   MLLSSCVLPSTAVASLSLSAKKIIISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFVFPY 60

Query: 61  SNYKRVHSSAISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELLN 120
           S YKRVHS AISS+MT+DTST++S DESMENIGILSHDPGL+PFKDHFKYRV RY +LLN
Sbjct: 61  STYKRVHSPAISSVMTEDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKNE 180
           LL+K+EGGLDEFARGYLKFGFNREE GI YREWAPAAQEAQIVGDFNGWDG+ H M+KNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180

Query: 181 YGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVY 240
           +G+WSIK+ D G +PAI+HNSRVKFRFKHG+GVWIDRIPAWIKYAT DPT+FAAPYDGVY
Sbjct: 181 FGIWSIKVYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPPLERYEFKHPRPTKPEAPRVYD---------------------------------- 300
           WDPPPLERYEFKHPRP KP APRVY+                                  
Sbjct: 241 WDPPPLERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNTV 300

Query: 301 ------------------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360
                                     RSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFD 420
           VTDGLNG+DVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EY+FD
Sbjct: 361 VTDGLNGFDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAE 480
           GFRFDGVTSMLY HHGINM F+GNY EYFSEATDVDAVVYLMLANNL +SILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFSGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSM EISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600
           C+SYAESHDQ+IVGDKTIAFLLMDKEMYSGMSCL  ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDD 660
           EGYLNFMGNEF HPEWIDFPREGN WSYDKCRRQWNL  TDHLRYKFLNAFD AMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDSAMNALDE 660

Query: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVS T EEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKEE 780
           DA DFGGHGRVGHD+DHFTSPEGIPG+PETNFNNRPNSFK+LSPARTCVVYY+VDESK E
Sbjct: 721 DASDFGGHGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKILSPARTCVVYYKVDESK-E 780

Query: 781 KENGDSVGANKPLATEVLARQVEEDYEGLTRPK-EGDIVAGEITNVED---DTSKIEDGD 791
           KE  D VG+      +V AR VEED EGL   K E DI  GEI+  ED   DTSK ED D
Sbjct: 781 KEKDDLVGSAN---EDVFARHVEEDSEGLAGCKEENDIAVGEISKTEDDDIDTSKPEDDD 840

BLAST of Sgr018488 vs. ExPASy TrEMBL
Match: A0A6J1KG03 (1,4-alpha-glucan branching enzyme OS=Cucurbita maxima OX=3661 GN=LOC111493933 PE=3 SV=1)

HSP 1 Score: 1345.5 bits (3481), Expect = 0.0e+00
Identity = 661/851 (77.67%), Postives = 715/851 (84.02%), Query Frame = 0

Query: 1   MLASPSVLPPSALASLSLSAKKNITTDSRNKCPSLRQQIELIFGSRMSHHRQMNPFMHPI 60
           MLASP VLP + LASLSLSAKKN T+DSRN+C SLRQQI L FGSRMSHHRQM+PFM P 
Sbjct: 1   MLASPCVLPSTVLASLSLSAKKNFTSDSRNQCSSLRQQIVLNFGSRMSHHRQMHPFMDPF 60

Query: 61  SNYKRVHSSA-ISSIMTDDTSTLTSADESMENIGILSHDPGLEPFKDHFKYRVSRYAELL 120
           S YKRV+  A ISS+MTDDTST +SADE+M++IGIL HDP LEPFKDHFKYR+ RY+++L
Sbjct: 61  STYKRVYRPAIISSVMTDDTSTASSADENMKDIGILRHDPSLEPFKDHFKYRIGRYSDVL 120

Query: 121 NLLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAAQEAQIVGDFNGWDGSKHRMKKN 180
           NL +K+EGGLDEFARGYLKFGFNRE  GI YREWAP AQEAQI+GDFNGWDGS HRM+KN
Sbjct: 121 NLFDKHEGGLDEFARGYLKFGFNREGDGIVYREWAPVAQEAQIIGDFNGWDGSNHRMEKN 180

Query: 181 EYGVWSIKLPDCGDEPAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGV 240
           E+G+WSI +PD   +PAIAHNSRVKFRFKHG+G WIDRIPAWIKYAT DPTRF APYDGV
Sbjct: 181 EFGIWSINIPDIDGKPAIAHNSRVKFRFKHGNGAWIDRIPAWIKYATVDPTRFGAPYDGV 240

Query: 241 YWDPPPLERYEFKHPRPTKPEAPRVYD--------------------------------- 300
           YW+PPPLERYEFKHPRP KP+APR+Y+                                 
Sbjct: 241 YWEPPPLERYEFKHPRPAKPKAPRIYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNT 300

Query: 301 -------------------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASN 360
                                      RSGTPEDLKYLIDKAHGLGLRVLMD+VHSHASN
Sbjct: 301 VQLMAIMEHSYYASFGYHVTNFFAMSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASN 360

Query: 361 NVTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKF 420
           NVTDGLNG+DVGQS+QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSN+RWWL+EY+F
Sbjct: 361 NVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQF 420

Query: 421 DGFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIA 480
           DGFRFDGVTSMLY HHGIN AFTGNY EYFSEATDVDAVVYLMLANNLI+SILP+ATVIA
Sbjct: 421 DGFRFDGVTSMLYHHHGINTAFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPNATVIA 480

Query: 481 EDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEISWNLTNRRYSE 540
           EDVSGMPGLG PVSEGGIGFDYRLQMAIPDKWIDY+KNK+DEEWSM EISWNLTNRRYSE
Sbjct: 481 EDVSGMPGLGIPVSEGGIGFDYRLQMAIPDKWIDYVKNKNDEEWSMGEISWNLTNRRYSE 540

Query: 541 KCVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALG 600
           KC+SYAESHDQ+IVGDKTIAFLLMDKEMYSGMSCLTEASP+VERGIALHKMIHFITMALG
Sbjct: 541 KCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTEASPVVERGIALHKMIHFITMALG 600

Query: 601 GEGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLD 660
           GEGYLNFMGNEF HPEWIDFPREGN WSYDKCRRQWNL  TDHLRYKFLNAFDRAMN LD
Sbjct: 601 GEGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNSLD 660

Query: 661 DKFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALD 720
           DKFSFLASSKQIVSCTSEE+KVIVFERGDLVFVFNFHP+NTYDGYKVGCD+PGKYRVALD
Sbjct: 661 DKFSFLASSKQIVSCTSEEEKVIVFERGDLVFVFNFHPINTYDGYKVGCDIPGKYRVALD 720

Query: 721 SDALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSFKVLSPARTCVVYYRVDESKE 780
           SDA +FGG GRVGHD+DHFTSPEGIPG+PETNFNNRPNSFKVLSPA+TCVVYYRVDESK 
Sbjct: 721 SDASEFGGRGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPAQTCVVYYRVDESK- 780

Query: 781 EKENGDSVGANKPLATEVLARQVEEDYEGLTRPKEGDIVAGEITNVEDDTSKIE-DGDVD 792
           EKENG SVGA++  AT+V+          L R KEG+I            SKI+ D DV 
Sbjct: 781 EKENGVSVGASEAHATDVV----------LARDKEGEI------------SKIKGDDDVH 828

BLAST of Sgr018488 vs. TAIR 10
Match: AT2G36390.1 (starch branching enzyme 2.1 )

HSP 1 Score: 716.1 bits (1847), Expect = 3.2e-206
Identity = 360/698 (51.58%), Postives = 450/698 (64.47%), Query Frame = 0

Query: 98  DPGLEPFKDHFKYRVSRYAELLNLLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAA 157
           DP L   ++H  YR  +Y +L   ++K EGGL+ F+RGY  FGF R   GI YREWAP A
Sbjct: 164 DPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTRSATGITYREWAPGA 223

Query: 158 QEAQIVGDFNGWDGSKHRMKKNEYGVWSIKLPDCGD-EPAIAHNSRVKFRFKHGDGVWID 217
           + A ++GDFN W+     M +N++GVW I LP+  D  PAI H SRVK R     G+  D
Sbjct: 224 KAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGSRVKIRMDTPSGI-KD 283

Query: 218 RIPAWIKYATADPTRFAAPYDGVYWDPPPLERYEFKHPRPTKPEAPRVYD---------- 277
            IPAWIKY+   P     PY+GVY+DPP  ++Y FKHPRP KP + R+Y+          
Sbjct: 284 SIPAWIKYSVQPPGEI--PYNGVYYDPPEEDKYAFKHPRPKKPTSLRIYESHVGMSSTEP 343

Query: 278 ------------------------------------------------QCRSGTPEDLKY 337
                                                             R GTP+DLK 
Sbjct: 344 KINTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKS 403

Query: 338 LIDKAHGLGLRVLMDVVHSHASNNVTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFN 397
           LIDKAH LGL VLMD+VHSHAS N  DGL+ +D    +   YFH+G RGYH +WDSRLFN
Sbjct: 404 LIDKAHELGLVVLMDIVHSHASKNTLDGLDMFD---GTDGQYFHSGSRGYHWMWDSRLFN 463

Query: 398 YANWEVLRFLLSNVRWWLDEYKFDGFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVD 457
           Y +WEVLR+LLSN RWWL+EYKFDGFRFDGVTSM+Y HHG+ + FTGNY EYF  +TDVD
Sbjct: 464 YGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYSTDVD 523

Query: 458 AVVYLMLANNLINSILPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLK 517
           AVVYLML N+LI+ + P+A V+ EDVSGMP    PV +GG+GFDYRL MA+ DKWI+ LK
Sbjct: 524 AVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVGFDYRLHMAVADKWIELLK 583

Query: 518 NKSDEEWSMEEISWNLTNRRYSEKCVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTE 577
            K DE+W + +I++ LTNRR+ EKCV YAESHDQA+VGDKTIAF LMDK+MY  M+   +
Sbjct: 584 -KRDEDWQVGDITFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRQ 643

Query: 578 ASPIVERGIALHKMIHFITMALGGEGYLNFMGNEFAHPEWIDFPR-----------EGNE 637
           A+P V+RGIALHKMI  ITM LGGEGYLNFMGNEF HPEWIDFPR            GN 
Sbjct: 644 ATPRVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRTDQHLPDGRVIAGNN 703

Query: 638 WSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSCTSEEDKVIVFE 697
            SYDKCRR+++L   ++LRY  L  FDRAM  L++ + F+ S  Q +S   E D+VIVFE
Sbjct: 704 GSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMTSEHQYISRKDEGDRVIVFE 763

Query: 698 RGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHDVDHFTSPEGIP 726
           RG+L+FVFNFH  N+Y  Y++GC +PGKY++ LDSD   FGG  R+    + FTS     
Sbjct: 764 RGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFGGFNRLDDSAEFFTS----- 823

BLAST of Sgr018488 vs. TAIR 10
Match: AT5G03650.1 (starch branching enzyme 2.2 )

HSP 1 Score: 712.2 bits (1837), Expect = 4.6e-205
Identity = 356/686 (51.90%), Postives = 448/686 (65.31%), Query Frame = 0

Query: 98  DPGLEPFKDHFKYRVSRYAELLNLLEKYEGGLDEFARGYLKFGFNREEGGIEYREWAPAA 157
           DP L  + +H  YR  +Y  L   ++KYEGGL+ F+RGY K GF+R + GI YREWAP A
Sbjct: 129 DPMLRTYNNHLDYRYGQYKRLREEIDKYEGGLEAFSRGYEKLGFSRSDAGITYREWAPGA 188

Query: 158 QEAQIVGDFNGWDGSKHRMKKNEYGVWSIKLPDCGD-EPAIAHNSRVKFRFKHGDGVWID 217
           + A ++GDFN W+ +   M +NE+GVW I LP+  D  PAI H SRVK R     G+  D
Sbjct: 189 KAASLIGDFNNWNSNADIMTRNEFGVWEIFLPNNTDGSPAIPHGSRVKIRMDTPSGI-KD 248

Query: 218 RIPAWIKYATADPTRFAAPYDGVYWDPPPLERYEFKHPRPTKPEAPRVYD---------- 277
            IPAWIK++   P     P++G+Y+DPP  E+Y FKHP+P +P++ R+Y+          
Sbjct: 249 SIPAWIKFSVQAPGEI--PFNGIYYDPPEEEKYVFKHPQPKRPKSLRIYEAHVGMSSTEP 308

Query: 278 ------------------------------------------------QCRSGTPEDLKY 337
                                                             R GTPE+LK 
Sbjct: 309 MVNTYANFRDDVLPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRCGTPEELKS 368

Query: 338 LIDKAHGLGLRVLMDVVHSHASNNVTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFN 397
           LID+AH LGL VLMD+VHSHAS N  DGLN +D    +   YFH+G RGYH +WDSRLFN
Sbjct: 369 LIDRAHELGLVVLMDIVHSHASKNTLDGLNMFD---GTDAHYFHSGPRGYHWMWDSRLFN 428

Query: 398 YANWEVLRFLLSNVRWWLDEYKFDGFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVD 457
           Y +WEVLR+LLSN RWWL+EYKFDGFRFDGVTSM+Y HHG+++ FTGNY EYF   TDVD
Sbjct: 429 YGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLSVGFTGNYTEYFGLETDVD 488

Query: 458 AVVYLMLANNLINSILPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLK 517
           AV YLML N++I+ + P+A  + EDVSGMP    PV +GG+GFDYRL MAI DKWI+ LK
Sbjct: 489 AVNYLMLVNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLK 548

Query: 518 NKSDEEWSMEEISWNLTNRRYSEKCVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTE 577
            K DE+W M +I + LTNRR+SEKC+SYAESHDQA+VGDKTIAF LMDK+MY  M+    
Sbjct: 549 -KRDEDWQMGDIIYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRP 608

Query: 578 ASPIVERGIALHKMIHFITMALGGEGYLNFMGNEFAHPEWIDFPR-----------EGNE 637
           ++P+++RGIALHKMI  ITM LGGEGYLNFMGNEF HPEWIDFPR            GN 
Sbjct: 609 STPLIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRGEQRLSDGSVIPGNN 668

Query: 638 WSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSCTSEEDKVIVFE 697
           +SYDKCRR+++L   D+LRY+ L  FD+AM  L++ + F+ S  Q +S   E D+VIVFE
Sbjct: 669 FSYDKCRRRFDLGDADYLRYRGLQEFDQAMQHLEENYGFMTSEHQFISRKDEADRVIVFE 728

Query: 698 RGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHDVDHFTSPEGIP 714
           RGDLVFVFNFH  ++Y  Y++GC  PGKY++ LDSD   FGG  R+    ++FT      
Sbjct: 729 RGDLVFVFNFHWTSSYFDYRIGCSKPGKYKIVLDSDDPLFGGFNRLDRKAEYFT----YD 788

BLAST of Sgr018488 vs. TAIR 10
Match: AT3G20440.1 (Alpha amylase family protein )

HSP 1 Score: 380.6 bits (976), Expect = 3.2e-105
Identity = 203/552 (36.78%), Postives = 314/552 (56.88%), Query Frame = 0

Query: 195 PAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVYWDPPPLERYEFKHP 254
           PA+ H S+ +  F   DG  ++R+PAW  Y    P         ++W+P P   Y++K+ 
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATY--VQPEDEGKQAYAIHWEPSPEAAYKWKYS 393

Query: 255 RPTKPEAPRVYD----------------------------QCRSGTPEDLKYLIDKAHGL 314
           +P  PE+ R+Y+                              R GTP+D K L+D+AHGL
Sbjct: 394 KPKVPESLRIYECHVGISGSEPKVSTFEEFTKKVTNFFAASSRYGTPDDFKRLVDEAHGL 453

Query: 315 GLRVLMDVVHSHASNNVTDGLNGYDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLR 374
           GL V +D+VHS+A+ +   GL+ +D    S D YFH G RG+HK W +R+F Y + +VL 
Sbjct: 454 GLLVFLDIVHSYAAADQMVGLSLFD---GSNDCYFHYGKRGHHKHWGTRMFKYGDLDVLH 513

Query: 375 FLLSNVRWWLDEYKFDGFRFDGVTSMLYQHHGINMAFTGNYKEYFSEATDVDAVVYLMLA 434
           FL+SN+ WW+ EY+ DG++F  + SM+Y H+G   +F  +  +Y ++  D DA++YL+LA
Sbjct: 514 FLISNLNWWITEYQVDGYQFHSLASMIYTHNGF-ASFNNDLDDYCNQYVDRDALMYLILA 573

Query: 435 NNLINSILPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWS 494
           N +++   P+   IAED +  PGL  PVS+GG+GFDY + ++  + W+  L N  D EWS
Sbjct: 574 NEILHVQHPNIITIAEDATYYPGLCEPVSQGGLGFDYYVNLSASEMWVSLLDNVPDNEWS 633

Query: 495 MEEI-SWNLTNRRYSEKCVSYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTEASP---- 554
           M +I S  + N+ Y++K +SYAE+H+Q+I G ++ A +L     + G+      SP    
Sbjct: 634 MSKIVSTLVANKEYADKMLSYAENHNQSISGGRSFAEIL-----FGGVD---NGSPGGKE 693

Query: 555 IVERGIALHKMIHFITMALGGEGYLNFMGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLH 614
           +++RGI+LHKMI  IT   GG  YLNFMGNEF HPE ++FP + N +S+    R+W+LL 
Sbjct: 694 LLDRGISLHKMIRLITFTSGGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLE 753

Query: 615 TDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVN 674
           +    + F  +FD+ +  LD     L+     +   ++ + VI F RG  +F+FNFHP N
Sbjct: 754 SGVHHHLF--SFDKELMDLDKSKGILSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSN 813

Query: 675 TYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHDVDHFTSPEGIPGLPETNFNNRPNSF 714
           +Y+ Y VG +  G+Y + L+SD + +GG G V    DH+     +        + + N  
Sbjct: 814 SYEKYDVGVEEAGEYTMILNSDEVKYGGQGIVTE--DHY-----LQRSISKRIDGQRNCL 861

BLAST of Sgr018488 vs. TAIR 10
Match: AT3G20440.2 (Alpha amylase family protein )

HSP 1 Score: 369.0 bits (946), Expect = 9.6e-102
Identity = 203/582 (34.88%), Postives = 314/582 (53.95%), Query Frame = 0

Query: 195 PAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRFAAPYDGVYWDPPPLERYEFKHP 254
           PA+ H S+ +  F   DG  ++R+PAW  Y    P         ++W+P P   Y++K+ 
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATY--VQPEDEGKQAYAIHWEPSPEAAYKWKYS 393

Query: 255 RPTKPEAPRVYD------------------------------------------------ 314
           +P  PE+ R+Y+                                                
Sbjct: 394 KPKVPESLRIYECHVGISGSEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTV 453

Query: 315 ----------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNNVTDGLNGYDVGQSS 374
                       R GTP+D K L+D+AHGLGL V +D+VHS+A+ +   GL+ +D    S
Sbjct: 454 GYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD---GS 513

Query: 375 QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFDGFRFDGVTSMLYQH 434
            D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EY+ DG++F  + SM+Y H
Sbjct: 514 NDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTH 573

Query: 435 HGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAEDVSGMPGLGRPVSE 494
           +G   +F  +  +Y ++  D DA++YL+LAN +++   P+   IAED +  PGL  PVS+
Sbjct: 574 NGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQ 633

Query: 495 GGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEI-SWNLTNRRYSEKCVSYAESHDQAIV 554
           GG+GFDY + ++  + W+  L N  D EWSM +I S  + N+ Y++K +SYAE+H+Q+I 
Sbjct: 634 GGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSIS 693

Query: 555 GDKTIAFLLMDKEMYSGMSCLTEASP----IVERGIALHKMIHFITMALGGEGYLNFMGN 614
           G ++ A +L     + G+      SP    +++RGI+LHKMI  IT   GG  YLNFMGN
Sbjct: 694 GGRSFAEIL-----FGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNFMGN 753

Query: 615 EFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNLLDDKFSFLASSK 674
           EF HPE ++FP + N +S+    R+W+LL +    + F  +FD+ +  LD     L+   
Sbjct: 754 EFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKELMDLDKSKGILSRGL 813

Query: 675 QIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHG 714
             +   ++ + VI F RG  +F+FNFHP N+Y+ Y VG +  G+Y + L+SD + +GG G
Sbjct: 814 PSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVKYGGQG 873

BLAST of Sgr018488 vs. TAIR 10
Match: AT3G20440.3 (Alpha amylase family protein )

HSP 1 Score: 363.6 bits (932), Expect = 4.0e-100
Identity = 197/550 (35.82%), Postives = 301/550 (54.73%), Query Frame = 0

Query: 195 PAIAHNSRVKFRFKHGDGVWIDRIPAWIKYATADPTRF---AAPYDGVYWDPPPLERYEF 254
           PA+ H S+ +  F   DG  ++R+PAW  Y     T F         ++W+P P   Y++
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATYVQPGMTAFEDEGKQAYAIHWEPSPEAAYKW 393

Query: 255 KHPRPTKPEAPRVYD--------------------------------------------- 314
           K+ +P  PE+ R+Y+                                             
Sbjct: 394 KYSKPKVPESLRIYECHVGISGSEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDY 453

Query: 315 -------------QCRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNNVTDGLNGYDVG 374
                          R GTP+D K L+D+AHGLGL V +D+VHS+A+ +   GL+ +D  
Sbjct: 454 FTVGYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD-- 513

Query: 375 QSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNVRWWLDEYKFDGFRFDGVTSML 434
             S D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EY+ DG++F  + SM+
Sbjct: 514 -GSNDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMI 573

Query: 435 YQHHGINMAFTGNYKEYFSEATDVDAVVYLMLANNLINSILPDATVIAEDVSGMPGLGRP 494
           Y H+G   +F  +  +Y ++  D DA++YL+LAN +++   P+   IAED +  PGL  P
Sbjct: 574 YTHNGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEP 633

Query: 495 VSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMEEI-SWNLTNRRYSEKCVSYAESHDQ 554
           VS+GG+GFDY + ++  + W+  L N  D EWSM +I S  + N+ Y++K +SYAE+H+Q
Sbjct: 634 VSQGGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQ 693

Query: 555 AIVGDKTIAFLLMDKEMYSGMSCLTEASP----IVERGIALHKMIHFITMALGGEGYLNF 614
           +I G ++ A +L     + G+      SP    +++RGI+LHKMI  IT   GG  YLNF
Sbjct: 694 SISGGRSFAEIL-----FGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNF 753

Query: 615 MGNEFAHPEWIDFPREGNEWSYDKCRRQWNLLHTDHLRYKFLNAFDRAMNL--LDDKFSF 674
           MGNEF HPE ++FP + N +S+    R+W+LL +    + F  +FD+   L  LD     
Sbjct: 754 MGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKVSELMDLDKSKGI 813

Query: 675 LASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALD 677
           L+     +   ++ + VI F RG  +F+FNFHP N+Y+ Y VG +  G+Y + L+SD + 
Sbjct: 814 LSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVK 868

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153149.10.0e+0082.171,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Momordica ... [more]
XP_038884508.10.0e+0080.051,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Benincasa ... [more]
XP_008464849.10.0e+0078.60PREDICTED: 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like ... [more]
XP_004144236.10.0e+0079.581,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic [Cucumis sativus... [more]
KAG6599100.10.0e+0078.031,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic, partial [Cucurb... [more]
Match NameE-valueIdentityDescription
Q410590.0e+0071.951,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic (Fragment) OS=Pi... [more]
P309240.0e+0070.491,4-alpha-glucan-branching enzyme OS=Solanum tuberosum OX=4113 GN=SBE1 PE=2 SV=2[more]
Q014012.0e-30668.251,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic OS=Oryza sativa su... [more]
Q080473.7e-20753.031,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic OS=Zea mays OX=4... [more]
O236474.5e-20551.581,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic OS=Arabidopsis... [more]
Match NameE-valueIdentityDescription
A0A6J1DI710.0e+0082.171,4-alpha-glucan branching enzyme OS=Momordica charantia OX=3673 GN=LOC111020717... [more]
E5GBZ30.0e+0078.601,4-alpha-glucan branching enzyme OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=... [more]
A0A1S3CMX90.0e+0078.601,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103502624 PE=3 S... [more]
A0A0A0KGQ90.0e+0079.581,4-alpha-glucan branching enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G357030 PE=... [more]
A0A6J1KG030.0e+0077.671,4-alpha-glucan branching enzyme OS=Cucurbita maxima OX=3661 GN=LOC111493933 PE... [more]
Match NameE-valueIdentityDescription
AT2G36390.13.2e-20651.58starch branching enzyme 2.1 [more]
AT5G03650.14.6e-20551.90starch branching enzyme 2.2 [more]
AT3G20440.13.2e-10536.78Alpha amylase family protein [more]
AT3G20440.29.6e-10234.88Alpha amylase family protein [more]
AT3G20440.34.0e-10035.82Alpha amylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 206..586
e-value: 0.0026
score: -81.3
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 271..324
e-value: 1.2E-5
score: 25.1
IPR0374391,4-alpha-glucan-branching enzymePIRSFPIRSF000463GlgBcoord: 266..726
e-value: 1.9E-141
score: 470.8
coord: 35..268
e-value: 2.8E-35
score: 119.8
IPR0374391,4-alpha-glucan-branching enzymePANTHERPTHR436511,4-ALPHA-GLUCAN-BRANCHING ENZYMEcoord: 25..266
IPR0374391,4-alpha-glucan-branching enzymePANTHERPTHR436511,4-ALPHA-GLUCAN-BRANCHING ENZYMEcoord: 268..732
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 134..248
e-value: 2.7E-39
score: 136.2
IPR004193Glycoside hydrolase, family 13, N-terminalPFAMPF02922CBM_48coord: 138..222
e-value: 1.1E-19
score: 70.5
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 266..608
e-value: 6.9E-168
score: 561.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 749..799
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 768..799
NoneNo IPR availablePANTHERPTHR43651:SF21,4-ALPHA-GLUCAN-BRANCHING ENZYME, CHLOROPLASTIC/AMYLOPLASTICcoord: 268..732
NoneNo IPR availablePANTHERPTHR43651:SF21,4-ALPHA-GLUCAN-BRANCHING ENZYME, CHLOROPLASTIC/AMYLOPLASTICcoord: 25..266
NoneNo IPR availableCDDcd02854E_set_GBE_euk_Ncoord: 145..241
e-value: 3.28465E-44
score: 152.302
NoneNo IPR availableCDDcd11321AmyAc_bac_euk_BEcoord: 245..595
e-value: 0.0
score: 673.947
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 610..717
IPR006048Alpha-amylase/branching enzyme, C-terminal all betaPFAMPF02806Alpha-amylase_Ccoord: 614..715
e-value: 6.4E-25
score: 87.5
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 609..725
e-value: 3.4E-44
score: 151.4
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 269..598
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 132..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr018488.1Sgr018488.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005978 glycogen biosynthetic process
biological_process GO:0019252 starch biosynthetic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0009501 amyloplast
cellular_component GO:0009507 chloroplast
molecular_function GO:0003844 1,4-alpha-glucan branching enzyme activity
molecular_function GO:0102752 1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis)
molecular_function GO:0043169 cation binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0003824 catalytic activity