Sgr017867 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr017867
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	DNA topoisomerase
Location	tig00153056: 828136 .. 830971 (+)
RNA-Seq Expression	Sgr017867
Synteny	Sgr017867

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCCAGCTTCAGAGGAGCTTGTTCTTGGCAACTACTTCCAGTAACGCCATATCTTCCTCTGTATTTTCAACAACTCCGTCTGGTTCATCCTCGCGGTTCGGTCCTTTCATGGCTAAGGTTTCATATTCTACCGGTTCCTCTTTGCAGCTTTGGCTGTTGTTTTGAAGTTTTCAACATTTATACTTTTCATTTCTGTCTAGAGAATTAATTGTCTGGCAGCAAATATTAGAGCTGAACTGTATGAAATTTTTTAACACTTATCTGGTAATGATCAGTAATATCTCCCGAGGAAAAGCGTTCGAAGAGTTGCAATTCCGTATATGCTTGAATTTTTGTTAACGTCATGCAAACTTCTGGTATTTGACAATTTTGGTTACTTCCGCTCACTGTTCTTTTTCTAAATGTTCTGGAGTGGCCTAGTGAATTTATATTTCTATTGCGGTGACGATGTGTTTCTATCAATCTCTCTTTGCAGTTTCGTATGTTGTTTTTGAATTCTTAATATTCATAGTCTTACATTCCTGTTTAGAGATTTGTCTCCCAGAAAATATTTGAGATGAACTGTATGAACATTTAAAAAACTAACTGGTATCGATCAATAATGTCCCCTAGGAAAAATGTACGAAAAGTTGCAATTCCATAAATGCTTGAATTTTTGTTAACGCCAAGCAAACTTCTGGCATTTGACAATTTTGGTTACTTCCGCTCACTTGTTCTTTTCCTAAATGTTTTGGAGTGGCCCAGTGAATTTATGTATATATATATATATTTTTTTGTGGTGATAATGTGTTATTTATTTGTGTTGTGTTACAGTTGCAGAGCAGGACTCTTCAAAATAATACGAGCAAATGCATACCTGTTTTACCTTTTACTACTGCTTGTAATTACGCTAGATGTTCTCAGCTTGCTTTTAGAAAAGGTGGAGCAACATTCTCTATCAAGACTGGACGTGCAAAGGCTGGGCAGTATTGTACATGTGATGGGCCAAATTGTGCTTTTACTCTATATTCCCGCTTGATATTGGGATTTCCCCATTCTGTTGCATCATCAAATGCTTCTAACCAACAGTCGTTAAATGGCAGTTTTACATCTAAACTTCCTTATTACAGAGCTTTTGGAAAGAAGTTCTTTTCTCAATTTACGGAAGCATGGAATGAAAGGGACAAAAAGAATGTTACATATGTAAGGAATGGAAGTACATTCCCATTTGAAGGATTGAATAAAAATGGAAGGGCACAAAGCCAGTTCTACTTCCAGGATAAAGAGAATTAATGCTTCAGGTACCTCAGGCTCCACTAGTGTTGGTGAAGCAAATTCAATCGAACAGTTGGTCGTGGCAGGAAGGAGGCAGATCTGGTTTCATCTAACTCTTCTTCATCCATTGGCAAAACATCCAAGGGTTTGAAAGAAAAGAAGACTGGAAGCAAGAAAAAGAAGGAAGCTATATCTTCAACCAATGCTGCAGATGTTGGAGCAGTATGCAAGGATGGTCGATCAAAGAGCTTAGATATTAGTGATAATAATGATCAGAATCTTGTACAAGATCCAAAGGTTAGCTAATAGTAATTGATGGATGTTCTCAATCCCCTTCCCCTCTCATTGCATTATTTTAACTAACTGTGCAGTTTTTCAGAACAAGTTACTGGGGATACTTCCAAAGGTTCTGCTTCAACGAAACAAAAATCCAATAAGAAAAGAGGTAACTCTAGCAGAAAAGGAGAAGCTGAAAAAGATGCTAAAAAGTTGCCACATAAGCAAAACCCTGCAGTGAATGGAAAGGGGAAACCTCAGGGACAAATGACTTTGAAGCAGCTATATCCCCCAGCGGGAAAGTCTGTTATGGTGGTGGAATCTGTGACAAAGGCAAAGGTTATTCAGAATTATCTAGGTGATATGTTCGAGGTTCTACCTAGCCATGGCCACGTTAGAGACTTGGCTGCAAGGTCGGATCTGTAAGACCTGATGATGACTTCAGTATGGTGTGGGAGGTTCCATCTGCTGCCTGGACTCATCTCAAGAGCATAAAAGTTTCCTTAAATGGGTATGCATCTAGATTTGGTTGTTACCTTGGCCTGCATGCAACTATATTTTGGCTAGCTTAATAATGATTGTTAAGTTTACATATGTATATGTATCTTTTATTTTTTTATTAACTTTTATTACTTCTTGTATATGGTTGACATTGTTGTCCAGCTCTATCATTTATTTTGTGATGTCTAATATCCCTTCTTTTCTTTTACTTTCAATTCCTTCAGGTTTAATTTTAATTTTCTTTTGCTTTGTTTTTATTCCTCAGAGCAGAGAACCTTATTCTTGCATCTGATCCTGACCGTGAAGGTGAGGCTATTGCTTGGCATATAATTGAGATGTTGCAGCAGCAGGATGCTCTACATGAAGGTATTAGCATAGCAAGGGTTGTTTTTCATGAAATAACTGAGGCTTCTATCAAGAGTGCTCTGCAATCTCCTAGAGGAATTGATGCAAACTTGGTTCAGGCTTACCTGCACGGCGTGCTTTAGATTATTTGATTGGATTTAATATTTCACCATTATTATGGAGGAAATTACCAGGTTGCCAATCAGCTGGGCGTGTTCAATCTGCAGCACTGGCCCTAATATGTGATAGAGAAATGGAAATTGACGAATTCCAACCACAAGAATATTGGACTGTAGAGATTGAGTTGAACCAAAAAGATCCCCATTCTTCAGTGGAAGAATTTCCATTCCCTGCACATTTGACCCACTTTGATTCTAAGAAGTTAAATCAGTTTGCAATTAGCTCCAACACAGAGGCAAAGAATATAGAAACAGCACTGAAATCAGTAAATTTTCAA

mRNA sequence

ATGAGCCAGCTTCAGAGGAGCTTGTTCTTGGCAACTACTTCCAGTAACGCCATATCTTCCTCTGTATTTTCAACAACTCCGTCTGGTTCATCCTCGCGGTTCGGTCCTTTCATGGCTAAGTTGCAGAGCAGGACTCTTCAAAATAATACGAGCAAATGCATACCTGTTTTACCTTTTACTACTGCTTGTAATTACGCTAGATGTTCTCAGCTTGCTTTTAGAAAAGGTGGAGCAACATTCTCTATCAAGACTGGACGTGCAAAGGCTGGGCAGTATTGTACATGTGATGGGCCAAATTGTGCTTTTACTCTATATTCCCGCTTGATATTGGGATTTCCCCATTCTGTTGCATCATCAAATGCTTCTAACCAACAGTCGTTAAATGGCAGTTTTACATCTAAACTTCCTTATTACAGAGCTTTTGGAAAGAAGTTCTTTTCTCAATTTACGGAAGCATGGAATGAAAGGGACAAAAAGAATGTTACATATGTAAGGAATGGAAGTACATTCCCATTTGAAGGATTGAATAAAAATGGAAGGGCACAAAGCCAGAAGGAGGCAGATCTGGTTTCATCTAACTCTTCTTCATCCATTGGCAAAACATCCAAGGGTTTGAAAGAAAAGAAGACTGGAAGCAAGAAAAAGAAGGAAGCTATATCTTCAACCAATGCTGCAGATGTTGGAGCAGTATGCAAGGATGGTCGATCAAAGAGCTTAGATATTAGTGATAATAATGATCAGAATCTTGTACAAGATCCAAAGAAAAGAGGTAACTCTAGCAGAAAAGGAGAAGCTGAAAAAGATGCTAAAAAGTTGCCACATAAGCAAAACCCTGCAGTGAATGGAAAGGGGAAACCTCAGGGACAAATGACTTTGAAGCAGCTATATCCCCCAGCGGGAAAGTCTGTTATGGTGGTGGAATCTGTGACAAAGGCAAAGGTTATTCAGAATTATCTAGGTGATATGTTCGAGGTTCTACCTAGCCATGGCCACGTTAGAGACTTGGCTGCAAGGTCGGATCTAGCAGAGAACCTTATTCTTGCATCTGATCCTGACCGTGAAGGTGAGGCTATTGCTTGGCATATAATTGAGATGTTGCAGCAGCAGGATGCTCTACATGAAGGCTTACCTGCACGGCGTGCTTTAGATTATTTGATTGGATTTAATATTTCACCATTATTATGGAGGAAATTACCAGGTTGCCAATCAGCTGGGCGTGTTCAATCTGCAGCACTGGCCCTAATATGTGATAGAGAAATGGAAATTGACGAATTCCAACCACAAGAATATTGGACTGTAGAGATTGAGTTGAACCAAAAAGATCCCCATTCTTCAGTGGAAGAATTTCCATTCCCTGCACATTTGACCCACTTTGATTCTAAGAAGTTAAATCAGTTTGCAATTAGCTCCAACACAGAGGCAAAGAATATAGAAACAGCACTGAAATCAGTAAATTTTCAA

Coding sequence (CDS)

ATGAGCCAGCTTCAGAGGAGCTTGTTCTTGGCAACTACTTCCAGTAACGCCATATCTTCCTCTGTATTTTCAACAACTCCGTCTGGTTCATCCTCGCGGTTCGGTCCTTTCATGGCTAAGTTGCAGAGCAGGACTCTTCAAAATAATACGAGCAAATGCATACCTGTTTTACCTTTTACTACTGCTTGTAATTACGCTAGATGTTCTCAGCTTGCTTTTAGAAAAGGTGGAGCAACATTCTCTATCAAGACTGGACGTGCAAAGGCTGGGCAGTATTGTACATGTGATGGGCCAAATTGTGCTTTTACTCTATATTCCCGCTTGATATTGGGATTTCCCCATTCTGTTGCATCATCAAATGCTTCTAACCAACAGTCGTTAAATGGCAGTTTTACATCTAAACTTCCTTATTACAGAGCTTTTGGAAAGAAGTTCTTTTCTCAATTTACGGAAGCATGGAATGAAAGGGACAAAAAGAATGTTACATATGTAAGGAATGGAAGTACATTCCCATTTGAAGGATTGAATAAAAATGGAAGGGCACAAAGCCAGAAGGAGGCAGATCTGGTTTCATCTAACTCTTCTTCATCCATTGGCAAAACATCCAAGGGTTTGAAAGAAAAGAAGACTGGAAGCAAGAAAAAGAAGGAAGCTATATCTTCAACCAATGCTGCAGATGTTGGAGCAGTATGCAAGGATGGTCGATCAAAGAGCTTAGATATTAGTGATAATAATGATCAGAATCTTGTACAAGATCCAAAGAAAAGAGGTAACTCTAGCAGAAAAGGAGAAGCTGAAAAAGATGCTAAAAAGTTGCCACATAAGCAAAACCCTGCAGTGAATGGAAAGGGGAAACCTCAGGGACAAATGACTTTGAAGCAGCTATATCCCCCAGCGGGAAAGTCTGTTATGGTGGTGGAATCTGTGACAAAGGCAAAGGTTATTCAGAATTATCTAGGTGATATGTTCGAGGTTCTACCTAGCCATGGCCACGTTAGAGACTTGGCTGCAAGGTCGGATCTAGCAGAGAACCTTATTCTTGCATCTGATCCTGACCGTGAAGGTGAGGCTATTGCTTGGCATATAATTGAGATGTTGCAGCAGCAGGATGCTCTACATGAAGGCTTACCTGCACGGCGTGCTTTAGATTATTTGATTGGATTTAATATTTCACCATTATTATGGAGGAAATTACCAGGTTGCCAATCAGCTGGGCGTGTTCAATCTGCAGCACTGGCCCTAATATGTGATAGAGAAATGGAAATTGACGAATTCCAACCACAAGAATATTGGACTGTAGAGATTGAGTTGAACCAAAAAGATCCCCATTCTTCAGTGGAAGAATTTCCATTCCCTGCACATTTGACCCACTTTGATTCTAAGAAGTTAAATCAGTTTGCAATTAGCTCCAACACAGAGGCAAAGAATATAGAAACAGCACTGAAATCAGTAAATTTTCAA

Protein sequence

MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFTTACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSNASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNGSTFPFEGLNKNGRAQSQKEADLVSSNSSSSIGKTSKGLKEKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDPKKRGNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVVESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARSDLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLPARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEIDEFQPQEYWTVEIELNQKDPHSSVEEFPFPAHLTHFDSKKLNQFAISSNTEAKNIETALKSVNFQ

Homology

[Close]

BLAST of Sgr017867 vs. NCBI nr
Match: XP_022144286.1 (uncharacterized protein LOC111013976 [Momordica charantia])

HSP 1 Score: 717.6 bits (1851), Expect = 7.2e-203
Identity = 416/604 (68.87%), Postives = 439/604 (72.68%), Query Frame = 0


Query: 1   MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFT 60
           MSQLQRSLFLATTSSNAISSS+ STTPS  SSRFGPFMAKLQSRTLQN TSKCIPVLPFT
Sbjct: 1   MSQLQRSLFLATTSSNAISSSLLSTTPSCPSSRFGPFMAKLQSRTLQNYTSKCIPVLPFT 60

Query: 61  TACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSN 120
           T CNYAR SQ+ FRKGGATFSIKT RAKAGQY +CDGPNCAFTLYSRL LGFPHSVA SN
Sbjct: 61  T-CNYARGSQVTFRKGGATFSIKTERAKAGQYLSCDGPNCAFTLYSRLTLGFPHSVAFSN 120

Query: 121 ASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNG-STFPFEGLNKNG 180
           ASNQ+ L+GSF+SKL  YRAFGKK FS  TEA NE D KNV YV NG S+ PF+GLNK  
Sbjct: 121 ASNQRLLDGSFSSKLLNYRAFGKKLFSHSTEARNESDNKNVPYVTNGISSLPFKGLNKKW 180

Query: 181 RAQS---------------------------------QKEADLVSSNSSSSIGKTSKGLK 240
           +                                     KEAD+VSSNSSSSIGK+SKGLK
Sbjct: 181 KGAKPVATSRTKRTSASGTSGSTSVDETNSSEQLVGVMKEADVVSSNSSSSIGKSSKGLK 240

Query: 241 EKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDPKKR---------- 300
           EKK+ S KKKEAISSTNAADVG V KDG SKSLD S  NDQNLVQDPKK+          
Sbjct: 241 EKKSRSNKKKEAISSTNAADVGVVSKDGPSKSLD-SSTNDQNLVQDPKKKSSGDTSKGSP 300

Query: 301 ----------GNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVV 360
                     GNS+RKGEA+KD  KLPHKQN  V+GKGKPQGQMTLKQLYPP GKSVMVV
Sbjct: 301 STKQQSTKKSGNSTRKGEAQKDTNKLPHKQNFVVDGKGKPQGQMTLKQLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS--------------------------- 420
           ESVTKAKVIQNYLGDMFEV+PSHGHVRDLAARS                           
Sbjct: 361 ESVTKAKVIQNYLGDMFEVIPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSIK 420

Query: 421 ---DLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLP------------------- 480
              + AENLILASDPDREGEAI+WHIIEMLQQQDALHEG+                    
Sbjct: 421 VSLNGAENLILASDPDREGEAISWHIIEMLQQQDALHEGISIARVVFHEITEASIKIALQ 480

Query: 481 --------------ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 488
                         ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID
Sbjct: 481 SPRGIDVNLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 540

[Close]

BLAST of Sgr017867 vs. NCBI nr
Match: XP_038897954.1 (DNA topoisomerase 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 670.2 bits (1728), Expect = 1.3e-188
Identity = 396/604 (65.56%), Postives = 421/604 (69.70%), Query Frame = 0


Query: 1   MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFT 60
           MSQLQRSL LAT+SSNAISSSVFSTTPS SSSR  PFMAKLQS T+QN TSKCIPVLPF+
Sbjct: 1   MSQLQRSLLLATSSSNAISSSVFSTTPSASSSRLRPFMAKLQSSTIQNYTSKCIPVLPFS 60

Query: 61  TACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSN 120
           T CNYARCSQL FRKG  TF+IKT RA AGQY T D PNC FTLYSRL LGF HSVAS N
Sbjct: 61  TTCNYARCSQLVFRKGRVTFAIKTERANAGQYRTRDEPNCVFTLYSRLPLGFVHSVASPN 120

Query: 121 ASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNG-STFPFEGLNKNG 180
           A N Q LNG+FT KLP YRAFGKKFFS+ TE  N  + KNV YVRNG ST PFEGLNK  
Sbjct: 121 APNWQLLNGTFTPKLPNYRAFGKKFFSKTTETLNGTENKNVKYVRNGTSTLPFEGLNKKW 180

Query: 181 RAQ---------------------------------SQKEADLVSSNSSSSIGKTSKGLK 240
           +                                   + KEA LVSS+SSSSI K SKGLK
Sbjct: 181 KGAKPVATSRLKRISASGPSGSTSVDGTNSSDQLVGTTKEAGLVSSDSSSSIRKASKGLK 240

Query: 241 EKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDP------------- 300
           EKK+GSKKKKEAISSTN ADV A CKDG+SKSLD S N DQ++VQDP             
Sbjct: 241 EKKSGSKKKKEAISSTNDADVEAACKDGQSKSLD-SSNIDQDVVQDPKKNVTGSTSKGSA 300

Query: 301 -------KKRGNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVV 360
                  KK+GNSSRK EA+KDA KLP K N  V+ +GK QG  T KQLYPP GKSVMVV
Sbjct: 301 STKQQSNKKKGNSSRKREAKKDANKLPQKPNFVVDARGKSQGITTFKQLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS--------------------------- 420
           ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS                           
Sbjct: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSIK 420

Query: 421 ---DLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLP------------------- 480
              + AENLILASDPDREGEAIAWHIIEML+QQDALHEG+                    
Sbjct: 421 VALNGAENLILASDPDREGEAIAWHIIEMLRQQDALHEGISIARVVFHEITETSIKSALQ 480

Query: 481 --------------ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 488
                         ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID
Sbjct: 481 SPRIIDTNLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 540

[Close]

BLAST of Sgr017867 vs. NCBI nr
Match: XP_038897953.1 (DNA topoisomerase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 670.2 bits (1728), Expect = 1.3e-188
Identity = 396/604 (65.56%), Postives = 421/604 (69.70%), Query Frame = 0


Query: 1   MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFT 60
           MSQLQRSL LAT+SSNAISSSVFSTTPS SSSR  PFMAKLQS T+QN TSKCIPVLPF+
Sbjct: 1   MSQLQRSLLLATSSSNAISSSVFSTTPSASSSRLRPFMAKLQSSTIQNYTSKCIPVLPFS 60

Query: 61  TACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSN 120
           T CNYARCSQL FRKG  TF+IKT RA AGQY T D PNC FTLYSRL LGF HSVAS N
Sbjct: 61  TTCNYARCSQLVFRKGRVTFAIKTERANAGQYRTRDEPNCVFTLYSRLPLGFVHSVASPN 120

Query: 121 ASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNG-STFPFEGLNKNG 180
           A N Q LNG+FT KLP YRAFGKKFFS+ TE  N  + KNV YVRNG ST PFEGLNK  
Sbjct: 121 APNWQLLNGTFTPKLPNYRAFGKKFFSKTTETLNGTENKNVKYVRNGTSTLPFEGLNKKW 180

Query: 181 RAQ---------------------------------SQKEADLVSSNSSSSIGKTSKGLK 240
           +                                   + KEA LVSS+SSSSI K SKGLK
Sbjct: 181 KGAKPVATSRLKRISASGPSGSTSVDGTNSSDQLVGTTKEAGLVSSDSSSSIRKASKGLK 240

Query: 241 EKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDP------------- 300
           EKK+GSKKKKEAISSTN ADV A CKDG+SKSLD S N DQ++VQDP             
Sbjct: 241 EKKSGSKKKKEAISSTNDADVEAACKDGQSKSLD-SSNIDQDVVQDPKKNVTGSTSKGSA 300

Query: 301 -------KKRGNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVV 360
                  KK+GNSSRK EA+KDA KLP K N  V+ +GK QG  T KQLYPP GKSVMVV
Sbjct: 301 STKQQSNKKKGNSSRKREAKKDANKLPQKPNFVVDARGKSQGITTFKQLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS--------------------------- 420
           ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS                           
Sbjct: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSIK 420

Query: 421 ---DLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLP------------------- 480
              + AENLILASDPDREGEAIAWHIIEML+QQDALHEG+                    
Sbjct: 421 VALNGAENLILASDPDREGEAIAWHIIEMLRQQDALHEGISIARVVFHEITETSIKSALQ 480

Query: 481 --------------ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 488
                         ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID
Sbjct: 481 SPRIIDTNLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 540

[Close]

BLAST of Sgr017867 vs. NCBI nr
Match: XP_022976242.1 (uncharacterized protein LOC111476696 isoform X1 [Cucurbita maxima])

HSP 1 Score: 667.9 bits (1722), Expect = 6.5e-188
Identity = 389/604 (64.40%), Postives = 421/604 (69.70%), Query Frame = 0


Query: 1   MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFT 60
           M QLQRSLF+ATTSSNA+SSSVFSTTPS S SR  P MAKLQSRT QNNTSKCIPVLPF+
Sbjct: 1   MRQLQRSLFMATTSSNAVSSSVFSTTPSASFSRLRPLMAKLQSRTSQNNTSKCIPVLPFS 60

Query: 61  TACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSN 120
           T CNYA+CSQL FRK   TF++KT RAKAGQ  TCDGP+C FTL+SRL LGF HSVAS N
Sbjct: 61  TVCNYAKCSQLVFRKSRVTFAVKTERAKAGQCRTCDGPSCVFTLHSRLPLGFHHSVASPN 120

Query: 121 ASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNG-STFPFEGLNKNG 180
           AS +Q LNG+FT   P YRAF KKFFSQ TEA N+ + +NV Y  NG STFPFEGLNK  
Sbjct: 121 ASKRQVLNGTFTPTFPNYRAFEKKFFSQSTEALNKTENENVKYASNGTSTFPFEGLNKKW 180

Query: 181 RAQS---------------------------------QKEADLVSSNSSSSIGKTSKGLK 240
           +                                     KEA LVSSNSSSS+ K SKGLK
Sbjct: 181 KGAKPVATSRKKQISASGSTGSTSVVGTNSSDQLVGVTKEAGLVSSNSSSSVRKASKGLK 240

Query: 241 EKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDP------------- 300
           EK +GSKKKKEAIS TN++DV AVCKD +SKSLD S N D+N+VQ+P             
Sbjct: 241 EKNSGSKKKKEAISPTNSSDVEAVCKDSQSKSLD-SSNVDKNVVQNPKKKSSGGTAKGSV 300

Query: 301 -------KKRGNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVV 360
                  KK+GNSSRK EA+KDA KLP K N  V+ +GK QG  TLKQLYPP GKSVMVV
Sbjct: 301 STTQQSNKKKGNSSRKKEAQKDASKLPQKPNLVVDARGKSQGITTLKQLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS--------------------------- 420
           ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS                           
Sbjct: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSMK 420

Query: 421 ---DLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLP------------------- 480
              + AENLILASDPDREGEAIAWHIIEMLQQQDALHEG+                    
Sbjct: 421 VSLNGAENLILASDPDREGEAIAWHIIEMLQQQDALHEGISIARVVFHEITEASIKSALQ 480

Query: 481 --------------ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 488
                         ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE+EI+
Sbjct: 481 SPRGIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRELEIE 540

[Close]

BLAST of Sgr017867 vs. NCBI nr
Match: XP_022936883.1 (uncharacterized protein LOC111443338 isoform X3 [Cucurbita moschata])

HSP 1 Score: 661.4 bits (1705), Expect = 6.1e-186
Identity = 386/604 (63.91%), Postives = 420/604 (69.54%), Query Frame = 0


Query: 1   MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFT 60
           M QLQRSLF+ATTSSNAISSSVFSTTPS S SR  P +AKLQSRT QNNTSKCIPVLPF+
Sbjct: 1   MRQLQRSLFMATTSSNAISSSVFSTTPSASFSRLRPLLAKLQSRTFQNNTSKCIPVLPFS 60

Query: 61  TACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSN 120
           T CNYA+CSQL FRK   TF++KT RAKAGQ  TCDGP+C FTL+SRL LGF HSVAS N
Sbjct: 61  TVCNYAKCSQLVFRKSRVTFAVKTERAKAGQCRTCDGPSCVFTLHSRLPLGFHHSVASPN 120

Query: 121 ASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNG-STFPFEGLNKNG 180
           AS +Q LNG+FT  LP YRAF KKFFSQ TEA N+ + +NV Y  NG STFP EGLNK  
Sbjct: 121 ASKRQVLNGTFTPTLPNYRAFEKKFFSQSTEALNKTENENVKYASNGTSTFPSEGLNKKS 180

Query: 181 RAQS---------------------------------QKEADLVSSNSSSSIGKTSKGLK 240
           +                                     KEA  VSSNSSSS+ K SKGLK
Sbjct: 181 KGAKPVATSRKKRISASGSTGSTSVVGTNSSDQLVGVTKEAGKVSSNSSSSVRKASKGLK 240

Query: 241 EKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDP------------- 300
           EK +GSKKKKEAIS TN++DV AVCKD +SKSLD S N D+N+VQ+P             
Sbjct: 241 EKNSGSKKKKEAISPTNSSDVEAVCKDSQSKSLD-SSNVDKNVVQNPKKKSSGGTAKGSV 300

Query: 301 -------KKRGNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVV 360
                  KK+GNSSRK EA+KDA KLP K N  V+ +GK +G  TLK+LYPP GKSVMVV
Sbjct: 301 STTQQSNKKKGNSSRKKEAQKDASKLPQKPNLVVDARGKSRGITTLKKLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS--------------------------- 420
           ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS                           
Sbjct: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSIK 420

Query: 421 ---DLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLP------------------- 480
              + AENLILASDPDREGEAIAWHIIEMLQQQDALHEG+                    
Sbjct: 421 VSLNGAENLILASDPDREGEAIAWHIIEMLQQQDALHEGISIARVVFHEITEASIKSALQ 480

Query: 481 --------------ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 488
                         ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE+EID
Sbjct: 481 SPREIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRELEID 540

[Close]

BLAST of Sgr017867 vs. ExPASy Swiss-Prot
Match: Q1RIM1 (DNA topoisomerase 1 OS=Rickettsia bellii (strain RML369-C) OX=336407 GN=topA PE=3 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.8e-39
Identity = 94/247 (38.06%), Postives = 135/247 (54.66%), Query Frame = 0


Query: 303 VMVVESVTKAKVIQNYLGDMFEVLPSHGHVRDLAAR------------------------ 362
           +++VES  KAK I  YLGD F+V+ S GH+RDL ++                        
Sbjct: 3   LVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVIPDENFSMKYDISEKAGKYV 62

Query: 363 ------SDLAENLILASDPDREGEAIAWHIIEMLQQQDA--------------------- 422
                 +  AE++ LA+DPDREGE+I+WHI E++++++                      
Sbjct: 63  DAIIKDAKKAESVYLATDPDREGESISWHIAEVIKEKNKVKSDDFFKRVAFNEITKKAIT 122

Query: 423 ------------LHEGLPARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE 482
                       L     ARRALDYL+GF +SPLLWRKLPGC+SAGRVQS AL LIC+RE
Sbjct: 123 HAIENPRKLDNNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICERE 182

Query: 483 MEIDEFQPQEYWTVEIELNQKDPHSSVEEFPFPAHLTHFDSKKLNQFAISSNTEAKNIET 487
            EI+ F+ +EYW + +++   +         F A LTH + +KL +F+I+++ EAK++  
Sbjct: 183 DEIERFKSEEYWDISLKMLNSNNEL------FTAKLTHINDQKLEKFSITNDKEAKDLTE 242

[Close]

BLAST of Sgr017867 vs. ExPASy Swiss-Prot
Match: Q4UM42 (DNA topoisomerase 1 OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=topA PE=3 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 2.0e-38
Identity = 92/247 (37.25%), Postives = 132/247 (53.44%), Query Frame = 0


Query: 303 VMVVESVTKAKVIQNYLGDMFEVLPSHGHVRDLAAR------------------------ 362
           +++VES  KAK I  YLGD F+V+ S GH+RDL ++                        
Sbjct: 3   LVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGKYV 62

Query: 363 ------SDLAENLILASDPDREGEAIAWHIIEMLQQQDA--------------------- 422
                 +  A+ + LA+DPDREGEAI+WH+ E++++++                      
Sbjct: 63  DAIVKDAKKADAVYLATDPDREGEAISWHVAEVIKEKNKVKSDDFFKRVAFNEITKKAII 122

Query: 423 ------------LHEGLPARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE 482
                       L     ARRALDYL+GF +SPLLWRKLPGC+SAGRVQS AL LIC+RE
Sbjct: 123 HAVENPRKLDANLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICERE 182

Query: 483 MEIDEFQPQEYWTVEIELNQKDPHSSVEEFPFPAHLTHFDSKKLNQFAISSNTEAKNIET 487
            EI+ F+ +EYW + +++   +         F A LTH + +KL +F+I +  +AK++  
Sbjct: 183 DEIERFKSEEYWDISLKMQNSNNEL------FTAKLTHVNDQKLEKFSIINEKDAKDLTE 242

[Close]

BLAST of Sgr017867 vs. ExPASy Swiss-Prot
Match: Q68X45 (DNA topoisomerase 1 OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=topA PE=3 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 9.9e-38
Identity = 92/247 (37.25%), Postives = 130/247 (52.63%), Query Frame = 0


Query: 303 VMVVESVTKAKVIQNYLGDMFEVLPSHGHVRDLAAR------------------------ 362
           +++VES  KAK I  YLGD F+V+ S GH+RDL ++                        
Sbjct: 3   LVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDKNFAMEYDISDKASKYV 62

Query: 363 ------SDLAENLILASDPDREGEAIAWHIIEMLQQQDA--------------------- 422
                 +  AE + LA+DPDREGE+I+WH+ E++++++                      
Sbjct: 63  DAIVKYARKAEAVYLATDPDREGESISWHVAEVIKEKNKVESDDFFKRVAFNEITKKAII 122

Query: 423 ------------LHEGLPARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE 482
                       L     ARRALDYL+GF +SPLLWRKLPGC+SAGRVQS AL LICDRE
Sbjct: 123 HAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICDRE 182

Query: 483 MEIDEFQPQEYWTVEIELNQKDPHSSVEEFPFPAHLTHFDSKKLNQFAISSNTEAKNIET 487
            EI+ F+ +EYW + +++   +         F A LTH + +KL +F+I +  EAK++  
Sbjct: 183 DEIERFKAEEYWDISLKMQNSNNEL------FTAKLTHVNDQKLKKFSIINEKEAKDLTR 242

[Close]

BLAST of Sgr017867 vs. ExPASy Swiss-Prot
Match: Q9ZDK2 (DNA topoisomerase 1 OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=topA PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.7e-37
Identity = 94/247 (38.06%), Postives = 133/247 (53.85%), Query Frame = 0


Query: 303 VMVVESVTKAKVIQNYLGDMFEVLPSHGHVRDLAAR------------------------ 362
           +++VES  KAK I  YLGD F+V+ S GH+RDL ++                        
Sbjct: 3   LVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDKNFLMEYDISDKAGKYV 62

Query: 363 ------SDLAENLILASDPDREGEAIAWHIIEMLQQQDA--------------------- 422
                 +  AE + LA+DPDREGE+I+WH+ E++++++                      
Sbjct: 63  DAIVKEARKAEVVYLATDPDREGESISWHVAEVIKEKNKVESDDFFKRVAFNEITKKAIM 122

Query: 423 ------------LHEGLPARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE 482
                       L     ARRALDYL+GF +SPLLWRKLPGC+SAGRVQS AL LICDRE
Sbjct: 123 NAVANPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICDRE 182

Query: 483 MEIDEFQPQEYWTVEIELNQKDPHSSVEEFPFPAHLTHFDSKKLNQFAISSNTEAKNIET 487
            EI+ F+ +EYW  +I L  ++ ++ +    F A LTH + +KL +F+I +  EAK++  
Sbjct: 183 DEIERFKSEEYW--DISLKMQNSNNDL----FTAKLTHVNDQKLKKFSIINEKEAKDLTQ 242

[Close]

BLAST of Sgr017867 vs. ExPASy Swiss-Prot
Match: Q92IH1 (DNA topoisomerase 1 OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=topA PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.9e-37
Identity = 90/247 (36.44%), Postives = 130/247 (52.63%), Query Frame = 0


Query: 303 VMVVESVTKAKVIQNYLGDMFEVLPSHGHVRDLAAR------------------------ 362
           +++VES  KAK I  YLGD F+V+ S GH+RDL ++                        
Sbjct: 3   LVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGKYV 62

Query: 363 ------SDLAENLILASDPDREGEAIAWHIIEMLQQQDA--------------------- 422
                 +  A+ + LA+DPDREGE+I+WH+ E++++++                      
Sbjct: 63  DAIVKDAKKADAVYLATDPDREGESISWHVAEVIKEKNQVKSDDFFKRVAFNEITKKAII 122

Query: 423 ------------LHEGLPARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE 482
                       L     ARRALDYL+GF +SPLLWRKLPGC+SAGRVQS AL LIC+RE
Sbjct: 123 HAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICERE 182

Query: 483 MEIDEFQPQEYWTVEIELNQKDPHSSVEEFPFPAHLTHFDSKKLNQFAISSNTEAKNIET 487
            EI+ F+ +EYW + +++   +         F A LTH + +KL +F+I +   AK++  
Sbjct: 183 DEIERFKSEEYWDISLKMQNSNNEL------FTAKLTHVNDQKLEKFSIINEKNAKDLTK 242

[Close]

BLAST of Sgr017867 vs. ExPASy TrEMBL
Match: A0A6J1CSY4 (Omega-protein OS=Momordica charantia OX=3673 GN=LOC111013976 PE=3 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 3.5e-203
Identity = 416/604 (68.87%), Postives = 439/604 (72.68%), Query Frame = 0


Query: 1   MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFT 60
           MSQLQRSLFLATTSSNAISSS+ STTPS  SSRFGPFMAKLQSRTLQN TSKCIPVLPFT
Sbjct: 1   MSQLQRSLFLATTSSNAISSSLLSTTPSCPSSRFGPFMAKLQSRTLQNYTSKCIPVLPFT 60

Query: 61  TACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSN 120
           T CNYAR SQ+ FRKGGATFSIKT RAKAGQY +CDGPNCAFTLYSRL LGFPHSVA SN
Sbjct: 61  T-CNYARGSQVTFRKGGATFSIKTERAKAGQYLSCDGPNCAFTLYSRLTLGFPHSVAFSN 120

Query: 121 ASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNG-STFPFEGLNKNG 180
           ASNQ+ L+GSF+SKL  YRAFGKK FS  TEA NE D KNV YV NG S+ PF+GLNK  
Sbjct: 121 ASNQRLLDGSFSSKLLNYRAFGKKLFSHSTEARNESDNKNVPYVTNGISSLPFKGLNKKW 180

Query: 181 RAQS---------------------------------QKEADLVSSNSSSSIGKTSKGLK 240
           +                                     KEAD+VSSNSSSSIGK+SKGLK
Sbjct: 181 KGAKPVATSRTKRTSASGTSGSTSVDETNSSEQLVGVMKEADVVSSNSSSSIGKSSKGLK 240

Query: 241 EKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDPKKR---------- 300
           EKK+ S KKKEAISSTNAADVG V KDG SKSLD S  NDQNLVQDPKK+          
Sbjct: 241 EKKSRSNKKKEAISSTNAADVGVVSKDGPSKSLD-SSTNDQNLVQDPKKKSSGDTSKGSP 300

Query: 301 ----------GNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVV 360
                     GNS+RKGEA+KD  KLPHKQN  V+GKGKPQGQMTLKQLYPP GKSVMVV
Sbjct: 301 STKQQSTKKSGNSTRKGEAQKDTNKLPHKQNFVVDGKGKPQGQMTLKQLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS--------------------------- 420
           ESVTKAKVIQNYLGDMFEV+PSHGHVRDLAARS                           
Sbjct: 361 ESVTKAKVIQNYLGDMFEVIPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSIK 420

Query: 421 ---DLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLP------------------- 480
              + AENLILASDPDREGEAI+WHIIEMLQQQDALHEG+                    
Sbjct: 421 VSLNGAENLILASDPDREGEAISWHIIEMLQQQDALHEGISIARVVFHEITEASIKIALQ 480

Query: 481 --------------ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 488
                         ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID
Sbjct: 481 SPRGIDVNLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 540

[Close]

BLAST of Sgr017867 vs. ExPASy TrEMBL
Match: A0A6J1IF86 (Omega-protein OS=Cucurbita maxima OX=3661 GN=LOC111476696 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 3.2e-188
Identity = 389/604 (64.40%), Postives = 421/604 (69.70%), Query Frame = 0


Query: 1   MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFT 60
           M QLQRSLF+ATTSSNA+SSSVFSTTPS S SR  P MAKLQSRT QNNTSKCIPVLPF+
Sbjct: 1   MRQLQRSLFMATTSSNAVSSSVFSTTPSASFSRLRPLMAKLQSRTSQNNTSKCIPVLPFS 60

Query: 61  TACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSN 120
           T CNYA+CSQL FRK   TF++KT RAKAGQ  TCDGP+C FTL+SRL LGF HSVAS N
Sbjct: 61  TVCNYAKCSQLVFRKSRVTFAVKTERAKAGQCRTCDGPSCVFTLHSRLPLGFHHSVASPN 120

Query: 121 ASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNG-STFPFEGLNKNG 180
           AS +Q LNG+FT   P YRAF KKFFSQ TEA N+ + +NV Y  NG STFPFEGLNK  
Sbjct: 121 ASKRQVLNGTFTPTFPNYRAFEKKFFSQSTEALNKTENENVKYASNGTSTFPFEGLNKKW 180

Query: 181 RAQS---------------------------------QKEADLVSSNSSSSIGKTSKGLK 240
           +                                     KEA LVSSNSSSS+ K SKGLK
Sbjct: 181 KGAKPVATSRKKQISASGSTGSTSVVGTNSSDQLVGVTKEAGLVSSNSSSSVRKASKGLK 240

Query: 241 EKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDP------------- 300
           EK +GSKKKKEAIS TN++DV AVCKD +SKSLD S N D+N+VQ+P             
Sbjct: 241 EKNSGSKKKKEAISPTNSSDVEAVCKDSQSKSLD-SSNVDKNVVQNPKKKSSGGTAKGSV 300

Query: 301 -------KKRGNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVV 360
                  KK+GNSSRK EA+KDA KLP K N  V+ +GK QG  TLKQLYPP GKSVMVV
Sbjct: 301 STTQQSNKKKGNSSRKKEAQKDASKLPQKPNLVVDARGKSQGITTLKQLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS--------------------------- 420
           ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS                           
Sbjct: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSMK 420

Query: 421 ---DLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLP------------------- 480
              + AENLILASDPDREGEAIAWHIIEMLQQQDALHEG+                    
Sbjct: 421 VSLNGAENLILASDPDREGEAIAWHIIEMLQQQDALHEGISIARVVFHEITEASIKSALQ 480

Query: 481 --------------ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 488
                         ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE+EI+
Sbjct: 481 SPRGIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRELEIE 540

[Close]

BLAST of Sgr017867 vs. ExPASy TrEMBL
Match: A0A6J1FEH1 (DNA topoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111443338 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 3.0e-186
Identity = 386/604 (63.91%), Postives = 420/604 (69.54%), Query Frame = 0


Query: 1   MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFT 60
           M QLQRSLF+ATTSSNAISSSVFSTTPS S SR  P +AKLQSRT QNNTSKCIPVLPF+
Sbjct: 1   MRQLQRSLFMATTSSNAISSSVFSTTPSASFSRLRPLLAKLQSRTFQNNTSKCIPVLPFS 60

Query: 61  TACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSN 120
           T CNYA+CSQL FRK   TF++KT RAKAGQ  TCDGP+C FTL+SRL LGF HSVAS N
Sbjct: 61  TVCNYAKCSQLVFRKSRVTFAVKTERAKAGQCRTCDGPSCVFTLHSRLPLGFHHSVASPN 120

Query: 121 ASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNG-STFPFEGLNKNG 180
           AS +Q LNG+FT  LP YRAF KKFFSQ TEA N+ + +NV Y  NG STFP EGLNK  
Sbjct: 121 ASKRQVLNGTFTPTLPNYRAFEKKFFSQSTEALNKTENENVKYASNGTSTFPSEGLNKKS 180

Query: 181 RAQS---------------------------------QKEADLVSSNSSSSIGKTSKGLK 240
           +                                     KEA  VSSNSSSS+ K SKGLK
Sbjct: 181 KGAKPVATSRKKRISASGSTGSTSVVGTNSSDQLVGVTKEAGKVSSNSSSSVRKASKGLK 240

Query: 241 EKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDP------------- 300
           EK +GSKKKKEAIS TN++DV AVCKD +SKSLD S N D+N+VQ+P             
Sbjct: 241 EKNSGSKKKKEAISPTNSSDVEAVCKDSQSKSLD-SSNVDKNVVQNPKKKSSGGTAKGSV 300

Query: 301 -------KKRGNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVV 360
                  KK+GNSSRK EA+KDA KLP K N  V+ +GK +G  TLK+LYPP GKSVMVV
Sbjct: 301 STTQQSNKKKGNSSRKKEAQKDASKLPQKPNLVVDARGKSRGITTLKKLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS--------------------------- 420
           ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS                           
Sbjct: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSIK 420

Query: 421 ---DLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLP------------------- 480
              + AENLILASDPDREGEAIAWHIIEMLQQQDALHEG+                    
Sbjct: 421 VSLNGAENLILASDPDREGEAIAWHIIEMLQQQDALHEGISIARVVFHEITEASIKSALQ 480

Query: 481 --------------ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 488
                         ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE+EID
Sbjct: 481 SPREIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRELEID 540

[Close]

BLAST of Sgr017867 vs. ExPASy TrEMBL
Match: A0A6J1F8P8 (Omega-protein OS=Cucurbita moschata OX=3662 GN=LOC111443338 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 3.0e-186
Identity = 386/604 (63.91%), Postives = 420/604 (69.54%), Query Frame = 0


Query: 1   MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFT 60
           M QLQRSLF+ATTSSNAISSSVFSTTPS S SR  P +AKLQSRT QNNTSKCIPVLPF+
Sbjct: 1   MRQLQRSLFMATTSSNAISSSVFSTTPSASFSRLRPLLAKLQSRTFQNNTSKCIPVLPFS 60

Query: 61  TACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSN 120
           T CNYA+CSQL FRK   TF++KT RAKAGQ  TCDGP+C FTL+SRL LGF HSVAS N
Sbjct: 61  TVCNYAKCSQLVFRKSRVTFAVKTERAKAGQCRTCDGPSCVFTLHSRLPLGFHHSVASPN 120

Query: 121 ASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNG-STFPFEGLNKNG 180
           AS +Q LNG+FT  LP YRAF KKFFSQ TEA N+ + +NV Y  NG STFP EGLNK  
Sbjct: 121 ASKRQVLNGTFTPTLPNYRAFEKKFFSQSTEALNKTENENVKYASNGTSTFPSEGLNKKS 180

Query: 181 RAQS---------------------------------QKEADLVSSNSSSSIGKTSKGLK 240
           +                                     KEA  VSSNSSSS+ K SKGLK
Sbjct: 181 KGAKPVATSRKKRISASGSTGSTSVVGTNSSDQLVGVTKEAGKVSSNSSSSVRKASKGLK 240

Query: 241 EKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDP------------- 300
           EK +GSKKKKEAIS TN++DV AVCKD +SKSLD S N D+N+VQ+P             
Sbjct: 241 EKNSGSKKKKEAISPTNSSDVEAVCKDSQSKSLD-SSNVDKNVVQNPKKKSSGGTAKGSV 300

Query: 301 -------KKRGNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVV 360
                  KK+GNSSRK EA+KDA KLP K N  V+ +GK +G  TLK+LYPP GKSVMVV
Sbjct: 301 STTQQSNKKKGNSSRKKEAQKDASKLPQKPNLVVDARGKSRGITTLKKLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS--------------------------- 420
           ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS                           
Sbjct: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSIK 420

Query: 421 ---DLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLP------------------- 480
              + AENLILASDPDREGEAIAWHIIEMLQQQDALHEG+                    
Sbjct: 421 VSLNGAENLILASDPDREGEAIAWHIIEMLQQQDALHEGISIARVVFHEITEASIKSALQ 480

Query: 481 --------------ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 488
                         ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE+EID
Sbjct: 481 SPREIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRELEID 540

[Close]

BLAST of Sgr017867 vs. ExPASy TrEMBL
Match: A0A6J1IMZ7 (Omega-protein OS=Cucurbita maxima OX=3661 GN=LOC111476696 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 8.9e-167
Identity = 363/604 (60.10%), Postives = 393/604 (65.07%), Query Frame = 0


Query: 1   MSQLQRSLFLATTSSNAISSSVFSTTPSGSSSRFGPFMAKLQSRTLQNNTSKCIPVLPFT 60
           M QLQRSLF+ATTSSNA+SSSVFSTTPS S SR  P MAK                    
Sbjct: 1   MRQLQRSLFMATTSSNAVSSSVFSTTPSASFSRLRPLMAK-------------------- 60

Query: 61  TACNYARCSQLAFRKGGATFSIKTGRAKAGQYCTCDGPNCAFTLYSRLILGFPHSVASSN 120
                     L FRK   TF++KT RAKAGQ  TCDGP+C FTL+SRL LGF HSVAS N
Sbjct: 61  ----------LVFRKSRVTFAVKTERAKAGQCRTCDGPSCVFTLHSRLPLGFHHSVASPN 120

Query: 121 ASNQQSLNGSFTSKLPYYRAFGKKFFSQFTEAWNERDKKNVTYVRNG-STFPFEGLNKNG 180
           AS +Q LNG+FT   P YRAF KKFFSQ TEA N+ + +NV Y  NG STFPFEGLNK  
Sbjct: 121 ASKRQVLNGTFTPTFPNYRAFEKKFFSQSTEALNKTENENVKYASNGTSTFPFEGLNKKW 180

Query: 181 RAQS---------------------------------QKEADLVSSNSSSSIGKTSKGLK 240
           +                                     KEA LVSSNSSSS+ K SKGLK
Sbjct: 181 KGAKPVATSRKKQISASGSTGSTSVVGTNSSDQLVGVTKEAGLVSSNSSSSVRKASKGLK 240

Query: 241 EKKTGSKKKKEAISSTNAADVGAVCKDGRSKSLDISDNNDQNLVQDP------------- 300
           EK +GSKKKKEAIS TN++DV AVCKD +SKSLD S N D+N+VQ+P             
Sbjct: 241 EKNSGSKKKKEAISPTNSSDVEAVCKDSQSKSLD-SSNVDKNVVQNPKKKSSGGTAKGSV 300

Query: 301 -------KKRGNSSRKGEAEKDAKKLPHKQNPAVNGKGKPQGQMTLKQLYPPAGKSVMVV 360
                  KK+GNSSRK EA+KDA KLP K N  V+ +GK QG  TLKQLYPP GKSVMVV
Sbjct: 301 STTQQSNKKKGNSSRKKEAQKDASKLPQKPNLVVDARGKSQGITTLKQLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS--------------------------- 420
           ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARS                           
Sbjct: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSMK 420

Query: 421 ---DLAENLILASDPDREGEAIAWHIIEMLQQQDALHEGLP------------------- 480
              + AENLILASDPDREGEAIAWHIIEMLQQQDALHEG+                    
Sbjct: 421 VSLNGAENLILASDPDREGEAIAWHIIEMLQQQDALHEGISIARVVFHEITEASIKSALQ 480

Query: 481 --------------ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDREMEID 488
                         ARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRE+EI+
Sbjct: 481 SPRGIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRELEIE 540

[Close]

BLAST of Sgr017867 vs. TAIR 10
Match: AT4G31210.1 (DNA topoisomerase, type IA, core )

HSP 1 Score: 248.8 bits (634), Expect = 8.8e-66
Identity = 175/392 (44.64%), Postives = 216/392 (55.10%), Query Frame = 0


Query: 179 GRAQSQKEADLVSSNSSSSIGKTSKGLKEKKTGSKKKKEAISSTNAADVGAVCKD----- 238
           G   S  +A+L ++ S +S G  +  +K K+    KK E  SS+    + AV  D     
Sbjct: 331 GDGSSGIKAELSTAASPASNGNQATTVKSKRRPKNKKVEDKSSSVVPVLEAVSLDESPIS 390

Query: 239 ---------GRSKSLDISDNNDQN-LVQDPKKRGNSSRKGEAE----KDAKKLPHKQNPA 298
                    G  KS        +N  V++PK    S+ K E +      A K P ++   
Sbjct: 391 VPKPKHSGSGNRKSSSAKKEVAKNHPVEEPKSPAPSNSKSEQQHLKSTKASKAPKQKLVP 450

Query: 299 VNGKG--KPQGQMTLKQLYPPAGKSVMVVESVTKAKVIQNYLGDMFEVLPSHGHVRDLAA 358
            + K   + +GQ   K LYPP+GKSV+VVES+TKAK+IQ YLGDM+EVLPS+GH+RDLA 
Sbjct: 451 QHMKNSIEHRGQNASKPLYPPSGKSVIVVESMTKAKIIQGYLGDMYEVLPSYGHIRDLAT 510

Query: 359 RS------------------------------DLAENLILASDPDREGEAIAWHIIEMLQ 418
           RS                              + AENLILASDPDREGEAIAWHIIEMLQ
Sbjct: 511 RSGSVRPDDDFSMVWEVPSSAWTHIKSIKVALNGAENLILASDPDREGEAIAWHIIEMLQ 570

Query: 419 QQDALHEGLP---------------------------------ARRALDYLIGFNISPLL 478
           QQ ALHE +                                  ARRALDYLIGFNISPLL
Sbjct: 571 QQGALHESMTVARVVFHEITESAIKSALQSPREIDGDLVHAYLARRALDYLIGFNISPLL 630

Query: 479 WRKLPGCQSAGRVQSAALALICDREMEIDEFQPQEYWTVEIELNQKDPHSSVEEFPFPAH 487
           WRKLPGC SAGRVQSAALAL+CDRE EID F+PQEYWTV I++  KD  ++     F AH
Sbjct: 631 WRKLPGCPSAGRVQSAALALVCDRESEIDGFKPQEYWTVGIKVKGKDNSAT-----FSAH 690

The following BLAST results are available for this feature:

BLAST of Sgr017867 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144286.1	7.2e-203	68.87	uncharacterized protein LOC111013976 [Momordica charantia]	[more]
XP_038897954.1	1.3e-188	65.56	DNA topoisomerase 1 isoform X2 [Benincasa hispida]	[more]
XP_038897953.1	1.3e-188	65.56	DNA topoisomerase 1 isoform X1 [Benincasa hispida]	[more]
XP_022976242.1	6.5e-188	64.40	uncharacterized protein LOC111476696 isoform X1 [Cucurbita maxima]	[more]
XP_022936883.1	6.1e-186	63.91	uncharacterized protein LOC111443338 isoform X3 [Cucurbita moschata]	[more]

BLAST of Sgr017867 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1RIM1	1.8e-39	38.06	DNA topoisomerase 1 OS=Rickettsia bellii (strain RML369-C) OX=336407 GN=topA PE=...	[more]
Q4UM42	2.0e-38	37.25	DNA topoisomerase 1 OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315...	[more]
Q68X45	9.9e-38	37.25	DNA topoisomerase 1 OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257...	[more]
Q9ZDK2	1.7e-37	38.06	DNA topoisomerase 1 OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=topA...	[more]
Q92IH1	4.9e-37	36.44	DNA topoisomerase 1 OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272...	[more]

BLAST of Sgr017867 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CSY4	3.5e-203	68.87	Omega-protein OS=Momordica charantia OX=3673 GN=LOC111013976 PE=3 SV=1	[more]
A0A6J1IF86	3.2e-188	64.40	Omega-protein OS=Cucurbita maxima OX=3661 GN=LOC111476696 PE=3 SV=1	[more]
A0A6J1FEH1	3.0e-186	63.91	DNA topoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111443338 PE=3 SV=1	[more]
A0A6J1F8P8	3.0e-186	63.91	Omega-protein OS=Cucurbita moschata OX=3662 GN=LOC111443338 PE=3 SV=1	[more]
A0A6J1IMZ7	8.9e-167	60.10	Omega-protein OS=Cucurbita maxima OX=3661 GN=LOC111476696 PE=3 SV=1	[more]

BLAST of Sgr017867 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31210.1	8.8e-66	44.64	DNA topoisomerase, type IA, core	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006171	TOPRIM domain	SMART	SM00493	toprim5	coord: 301..371 e-value: 9.6E-17 score: 71.6
IPR006171	TOPRIM domain	PFAM	PF01751	Toprim	coord: 303..368 e-value: 4.2E-14 score: 52.6
IPR006171	TOPRIM domain	PROSITE	PS50880	TOPRIM	coord: 301..387 score: 20.330379
IPR003601	DNA topoisomerase, type IA, domain 2	SMART	SM00436	topIban2	coord: 354..432 e-value: 4.1E-13 score: 59.5
None	No IPR available	GENE3D	3.40.50.140		coord: 298..339 e-value: 7.1E-7 score: 31.2
None	No IPR available	GENE3D	3.40.50.140		coord: 340..379 e-value: 4.8E-9 score: 38.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 202..220
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 255..274
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 173..201
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 173..223
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 235..292
None	No IPR available	PANTHER	PTHR42785:SF4	BNAA01G05990D PROTEIN	coord: 342..375
None	No IPR available	PANTHER	PTHR42785:SF4	BNAA01G05990D PROTEIN	coord: 378..486
None	No IPR available	PANTHER	PTHR42785:SF4	BNAA01G05990D PROTEIN	coord: 168..339
IPR013824	DNA topoisomerase, type IA, central region, subdomain 1	GENE3D	1.10.460.10	Topoisomerase I, domain 2	coord: 380..484 e-value: 3.3E-19 score: 71.4
IPR013497	DNA topoisomerase, type IA, central	PFAM	PF01131	Topoisom_bac	coord: 371..483 e-value: 7.8E-23 score: 81.2
IPR000380	DNA topoisomerase, type IA	PANTHER	PTHR42785	DNA TOPOISOMERASE, TYPE IA, CORE	coord: 378..486
IPR000380	DNA topoisomerase, type IA	PANTHER	PTHR42785	DNA TOPOISOMERASE, TYPE IA, CORE	coord: 342..375
IPR000380	DNA topoisomerase, type IA	PANTHER	PTHR42785	DNA TOPOISOMERASE, TYPE IA, CORE	coord: 168..339
IPR023405	DNA topoisomerase, type IA, core domain	SUPERFAMILY	56712	Prokaryotic type I DNA topoisomerase	coord: 301..478

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr017867.1	Sgr017867.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006265	DNA topological change
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003917	DNA topoisomerase type I (single strand cut, ATP-independent) activity
molecular_function	GO:0003916	DNA topoisomerase activity