Sgr017514 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr017514
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionDAO domain-containing protein
Locationtig00153048: 754703 .. 760928 (-)
RNA-Seq ExpressionSgr017514
SyntenySgr017514
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCTCTCTTTCTCAAGGTTCTATCCTTCAGTCTGCCGCTATGTTGTCAGCACTTTCTCTTTCTCAGCGTCCTCTGAGGTTCTCTCAACCTTCTTTTTGTTTCCATTTACTTGATTAACGCAGGTTTCTCAGTTTGTATCTCAAAATGTAGATGTATTTATTCAGAGAGTGATATCGTTTTATAGGTATGCTGTGCTTGGAGCTGGTTTTGCTGGGCTTTCAGTTACTTGGCATTTGTTGAAGGTACGGTTCTGTTACTCTGTTTTCCCTGTTTGTTTTATAAAACATCAAGAGTCTGCTTTGGCTGCATAGACTGTCTTCTTCTTCTTCAAAATTTTTTGGTTGTTGGATTGTGGTTTTTGTGCATCAGTTGGAAATAAAAATTTATTCTACTCATTGGGATTCATTCAAGGGATTTCTTACATTTTTATTTGTGTAGTGAATGTCTCTTTAAGATTAATGATTGCACGAGGACATATACTTCCATGAAATATATTCCCTCATTTAGTCAGCCCCCATTCAAGTTCCATTGAACCTTCTTTCATTTATTCGCTTATGGGTTTATATTCGGTGCCAATTGGGGGTTCTAGGGAAAAATGCCCTTGTGGGTATATCTCTTTGATTCAATGTCCCTAGGTTAATGCGATTTCTTTTTTAAGGTTTCTATAGAGTATTCTAGTATTAGTGTGACATTTTTTTAGGATGGCTTCTCTCCCAATATATATTGCCTATAATGGTTGATGGATAAATTTTGAAGGTCGATTGAGTATATTGGCCGTAAGGGAAAAGGAATGATTCTTCTACACAAATAGCACCTACTTGGACTTGGTCGAGAAGATTTACAATGTTACTAGAATAGAACGTAGGTATTATGATTTGAGGTTGAGTATTGCTAGCCCCCTTGAACACTTGCCACCCACAAACTTTGTTGATGATCATGGTTTGCAATTCTATCGTTTTCAAGTGGAACAAAAAAAGATGATGTACTATGCATTTCTGCTGAATTAAAAGTTGTGGAGCCTGATTCTTCAGATCAAAATCATACTCCCATAAAGGTTGAGACTGTACTTGCATGTGCTCGAAAAGCTTACAGCAGCTCCAGCCAGGGTAGTAACTCAACAAGAGTTGAAACCTTTAGTACTGAAATACATGGTGTAGCTCAAGGTGAATTTATCCATGATTTGAATGAACACGGCGTCGGTAGAATTTCTTCTTCTAGTTGGTTGAATGACTTGCCAAATTTTGATCAATGGGGTGAATATGAAGTAGATGAAGTACATGAGGTGTGTGAAGTGTCTAAATCTTCATATGAATTGAATAGCAAATCGAACACCCCTCTTAAAAACCCAAATGCTACAAAGGATTTTGCACTACTTCCGACATATGGATACACTATAAATGATAACAATATGTTTTTTTTATAGGAAAGTGATAACAATACATTTGAAAAAGATCAAGTTTTTTCGAGCAAGGAAGATTTCAAATATCGACTAATTGTATTTGCCATGAAAAATAGTTTCAAGTATAGAGTAAAAAAGTCTGAAAAGAAGCGGCTCTTTATAATTTGTATGGGTGACAATTGTCCATGGAGCTTACGAGCGATTAAGTAGCCAGAAACTACCTGTCTATGATTCCAAAGTTTTGTGACACTCATGACTGTTCATTACAAGTTAGAGATTGTTATCATAAACAAGCTTGTCGCTCAGTTATTGGTAGGTCTACGAAGTTTAAATATAAAGGGGCTGGACAAATTCAGCTCGGGATAAAGTTCATGACATGCATAGAGAAGAAAGAAATAGTACTAGATATGGCAAAGTGTGGCAAACACAAGATGTAGTTAAAATGGTTTCACCAAAGGCATCTTTTGGCAACCTTCCAAGATATTGTTTCACGTTAGAGCAAAAAATTTCAAGGACTATAACTCACATCCAGGTTAATGATTTAAATAACTTTCAATTTCTCTTCATCATTCTTGGTCCTTTTCTACGTGGGTTTAATAATTGTATTCGTTTAGTTATTGTAATTGATATGACACATTTGGCTGGTAAATATTGTGGAGTCCTATTAGTGCATGTTTGGATGGAAATAACCAAGTATACTCTTTAGTCTTTAGTATTGGGAATTATGAGGATGATCTTACATGGAAATGGTTTCTTACAAAATTACATGAAACGATTGGAGAAGTTTCTGATCTTGTCCTCATATCTAATTGGAAAGTGAGTCTTGTTAAAAGTGTAGCTTCTATTTTTCCTAATGAATTCAATGGGGCATGTTTATGCCATTTAACACAAAATTTGTATAACCAGTTCAAGAATGAGAAAATAAGCCTCTATTCCTAGTTGCTGCTAAGGAATATCGTCCATCAGACTTTGATAGATTATGGGAGGAAACTCAAAATATGGATGGTGAGATGACATTATTTGAGGGAAATAGATTTTCATGGATGGGCACGAGCTTATTTCACAAGGTGTCGATATAACATCATGACCATAAACATTACTTAAGCATTAAATCAGATCCTAGTCAATGCTTGGGTGTTACCTATTATAGCCTTAGTAGAGCATCTTAGAAGCTTACTACAAAGATGGTTCCATGATTAATGAATTTTGGCTATGAACCCAAACACCTCTACACACCGTGGGCAGAACAAATCTTGGAACAAAATAATGCAGAAGCCGTAACTATGTGTGCTCTGACCATCATAAGTTTCTTATTATAGATAGCGATCGAGATGACATCGTTAATATTCACATGAAGACATGCACATGTCATGAGTTTTATTTAGACCAAATTCCAAGTCGTCCTGCATTAATGTTATTCGAGAAAGGTGTATAAGTTCATATACACTTTGCTCAAATTATTATTCTAGTGAAGCACGACTTGCAGCTTATGCAGAAACAATCCTTCCTCTCGCCCACCAAATTGATTGGGTAATCCCTGAGCAAGTAGATTAAGTTGTCCTCTTACCTCCAATAGTATGAGCCTATGCAAGACATTATACGAGCAGAATTCCTAAGGTTGGAGAGACTCGAGCGTCACGATCTTCTTGTTAGTCTCGTCAGAAATGCCATAGTCGACAAACATGTAAAAACCGAAGTGCAGATCCTAGTACTGCTCAGTAGTAGTTTTTTTTTTTTTTTTTTTTGTCCTCTGTAACAACTTATGTAGTACCTTTTAAAGCTATGACTTATAAATGCACTAATTTAGTTGTAATAGATTAGTTCGAATATGGGAGTCTAATGCATGAAGAAAACTTTAAGTGATTATATCCAAATATATTTTTAATATTATAGGTTCTCTTTGTCATGATATGATACCATAATTTTGCACAATTCATTGAAGAAACTAATATCAGTCTTGAGATAAAAAGGAACGTGAACATAACATCACAAAAAACTCTATGAACTTGACGAGAAAAAAAAAAACACAATGCCTCATTTCCACCTTGTAGTGGAATGTACCTCTATTGTTCTAGATCTCATGGTTCCGAACTTTCTTGACATAAACTTTTTTCGGGTCAAAGAATCCATTTTGCTCTCTTAGGGAAGGTAACGTCTCCTCTGTAGGATCCATAATTTGCTCCATGCATTCTTGCGTTTCTTGCTCCTTGTTGATTTACGAGAGCCATACATTAATATTTTCCGTCCTTTAGGGTTGATGGATGCTAACACCAAATCTTTTTTTTTTTTGGTCTACAATGTTGGGAACATAAACCACAAATGATATTAAACATGATTAGAAAAAATATCAAGGCAGTGAAATATACATTAATATTTTTCGTTCTTTAGGGTGGAGGGCATTTTGCCTTTACATATTCTATCTTAAGGCAGTGAGCCCACTAACCTGCCAATGGGATCGAAAAATCATAACTTTTAGTTAATTGGACGGATTCCTGAGGTTACTTCCTCTAATGCATCAAGCTTGCATCAAATGATCGATCTAGCTTCTGCTGGTATACGTTACTTATTACCTAATATGTACTTCATCTTATTATGTTGAAATGATTATTGCAGCTCTGTCCTAGGGATGTACCTCTATGCATTGACATATATGACGAAGCTGGCATTGGTGGAGGGGCATCTGGAGTCGCTGGGGGCCTTCTTCACCCTTATTCTCCTAAAGGTTCCGACAGCCTAGTTAGTTACTTTACCTCCTCCACTGCGAAAATCGGCCTTTAGATGCATGTGGCTAACCCCTCGAAATTATGACAGTTAAACTTCTTTGGAAAGGTTCCGAGTGTTGGGCTGAGTGCTTAAAGCTTTTGAGCGTTGCAGAGTCGGCACTTCACTTCAAAGAGTTGGATTTTGAGATGGATAAGTGTCAGAATATGCATGGATTCATTGTTCTAAGAAGGTCTGTTACTTCTTTTTTTTTTAGCTTCCTAGACATTTATCCCTGATCTCTTGGGTCTTCTCCACAAAGATCCTCCTCCTTTGGTTATGTTGAAACAAATTATATCCATTGAGATGCATATATCCATTACATTATTGACATTTCCATCTTTTGTTCCCTTTTCATACTTGAGCATTTTCAAACAAGAATGTCTAGATATTCCCTTCAAAATCTGTTTAATTATCATTCTTTTGGAAATAGCAGATGATGCGCATGGCATGCTTATGGTGTATGTTATTTACTTCAGGGGCATCTTGAGGCCTGCAATCAGCTTGAAGAATTTGATGGTGTTAAAAGAAGTAATTATATCCTTTGCTTCTATATATGTTTCATTACTCTCACAAGGAGTCTTAACAGATGTTGATTACTTTATTTATGGAGCTAGAATGCTCAGAATGGCCTTGATAGCTGCAGAATAGAAACCATTGATGAAGATGCTGCCCGAAATCTTATTCCTCAGCTTTACGTGCCTTTAAATACAGCTTTTTATATGCCTCAAGCTGGAAATATCAATTCTCAGCGTTACCTTGAGGTCTCAACTTCTCGGATAATTGATTTTTCTTCCATTTGTTATATATACTATAACAATTCTCTGATCTCTTTCTTTCCACACTTATGGCTAGGCGCTATTTATAGCATGCGAAAATCTGATCAAGGAATCATCCACCTCAGGTTTTGGTCAAAAGGAATTATATTTGCAGAAGAAATCTATTAACGAGCTGCGTGAATTAGAAGGTAAGTGATATTTCTCAAACCAAATTTTCTCAGCATTACCATGGAATTGTATTGTTTCTTATATATTACAACTTTCTCGAAAGCTTGCAGGGGCATACGATGCTGTGATTGTTTGTCTAGGTGCCAAAATGGTCATGCTTCCTGAACTTTCTGGAAAGCTTCCATTGAGAACTTGCAGAGGTGTAGTTGCTCATTTGCAGCTACCGGATGATATCAGGTATCAGTTTCTTAAACCAAGTATGTGCTATCCCTCCACAAAATTCATAATTTGGGGCATCAGAATTACTACAGTATGTGGTGTTTGTTATTTATTATCATGAGCATTGTTACTCTGCATGCTTTTCGATCGGGCCAAAATTGCGTTTTCTCCCATGTTTGATTACTTGAGATACCAACTGCTAGTTTAGCCAATATGATCTTGTCTGAACAAGAGTGTCGAGATCAATTTTGACGTTCTTGGTCTGCTCAGATTATCCTGAAATAATTAGCGTAAACCGTCATGAAAAATCTTAGAAACTTTTCAAGAAACTTTGCTGTAAGAGTGTTCCAGGAAGATGCTGTGACTTTACATTTGCTGTTGAAAATCTTAAGAAACACCTTGATAATTTCTGATATCTGGTACAATATTGTATTTCAGCAATACATACCCAGAGCTTGGGCCTTCTATACTGTCAGATGCATGGCTTGCCATACAGGGTCCTAGGAGTTTATATATGGGTTCAACATGGGAATGGCAATCAACTAATTCATCACCCGAAGTTTCAGAAGATGAAGGCTCAAGAGCGCTCGCAGAACTTTTACCAAAGGTGTCTGCCATTTATCCCTCCATAAAGGAGTGGAGTTTCACAAGAGCAATGGCTGGTCTAAGAGCAATGCCACCACTTACTCCTCATGGATCTCTTCCTCTTTTAGGTTGTATAGACGAGATTGTAAGACATAACAGCTCTTGCAAGTATTGGTTATTTGGAGGGCTTGGTTCAAGGGGATTGTTGTACCATGGTTGGCTTGGGAAATTAACAGCGCAGGCTGTGCTTTCATGTAGTGAAGAACTTATTCCATCTGAACTAACCTCTTGGAAGAAAATAATGAATAGTTAA

mRNA sequence

ATGCTCTCTCTTTCTCAAGGTTCTATCCTTCAGTCTGCCGCTATGTTGTCAGCACTTTCTCTTTCTCAGCGTCCTCTGAGGTATGCTGTGCTTGGAGCTGGTTTTGCTGGGCTTTCAGTTACTTGGCATTTGTTGAAGCTCTGTCCTAGGGATGTACCTCTATGCATTGACATATATGACGAAGCTGGCATTGGTGGAGGGGCATCTGGAGTCGCTGGGGGCCTTCTTCACCCTTATTCTCCTAAAGTTAAACTTCTTTGGAAAGGTTCCGAGTGTTGGGCTGAGTGCTTAAAGCTTTTGAGCGTTGCAGAGTCGGCACTTCACTTCAAAGAGTTGGATTTTGAGATGGATAAGTGTCAGAATATGCATGGATTCATTGTTCTAAGAAGGGGCATCTTGAGGCCTGCAATCAGCTTGAAGAATTTGATGGTGTTAAAAGAAAATGCTCAGAATGGCCTTGATAGCTGCAGAATAGAAACCATTGATGAAGATGCTGCCCGAAATCTTATTCCTCAGCTTTACGTGCCTTTAAATACAGCTTTTTATATGCCTCAAGCTGGAAATATCAATTCTCAGCGTTACCTTGAGGCGCTATTTATAGCATGCGAAAATCTGATCAAGGAATCATCCACCTCAGGTTTTGGTCAAAAGGAATTATATTTGCAGAAGAAATCTATTAACGAGCTGCGTGAATTAGAAGGGGCATACGATGCTGTGATTGTTTGTCTAGGTGCCAAAATGGTCATGCTTCCTGAACTTTCTGGAAAGCTTCCATTGAGAACTTGCAGAGGTGTAGTTGCTCATTTGCAGCTACCGGATGATATCAGCAATACATACCCAGAGCTTGGGCCTTCTATACTGTCAGATGCATGGCTTGCCATACAGGGTCCTAGGAGTTTATATATGGGTTCAACATGGGAATGGCAATCAACTAATTCATCACCCGAAGTTTCAGAAGATGAAGGCTCAAGAGCGCTCGCAGAACTTTTACCAAAGGTGTCTGCCATTTATCCCTCCATAAAGGAGTGGAGTTTCACAAGAGCAATGGCTGGTCTAAGAGCAATGCCACCACTTACTCCTCATGGATCTCTTCCTCTTTTAGGTTGTATAGACGAGATTGTAAGACATAACAGCTCTTGCAAGTATTGGTTATTTGGAGGGCTTGGTTCAAGGGGATTGTTGTACCATGGTTGGCTTGGGAAATTAACAGCGCAGGCTGTGCTTTCATGTAGTGAAGAACTTATTCCATCTGAACTAACCTCTTGGAAGAAAATAATGAATAGTTAA

Coding sequence (CDS)

ATGCTCTCTCTTTCTCAAGGTTCTATCCTTCAGTCTGCCGCTATGTTGTCAGCACTTTCTCTTTCTCAGCGTCCTCTGAGGTATGCTGTGCTTGGAGCTGGTTTTGCTGGGCTTTCAGTTACTTGGCATTTGTTGAAGCTCTGTCCTAGGGATGTACCTCTATGCATTGACATATATGACGAAGCTGGCATTGGTGGAGGGGCATCTGGAGTCGCTGGGGGCCTTCTTCACCCTTATTCTCCTAAAGTTAAACTTCTTTGGAAAGGTTCCGAGTGTTGGGCTGAGTGCTTAAAGCTTTTGAGCGTTGCAGAGTCGGCACTTCACTTCAAAGAGTTGGATTTTGAGATGGATAAGTGTCAGAATATGCATGGATTCATTGTTCTAAGAAGGGGCATCTTGAGGCCTGCAATCAGCTTGAAGAATTTGATGGTGTTAAAAGAAAATGCTCAGAATGGCCTTGATAGCTGCAGAATAGAAACCATTGATGAAGATGCTGCCCGAAATCTTATTCCTCAGCTTTACGTGCCTTTAAATACAGCTTTTTATATGCCTCAAGCTGGAAATATCAATTCTCAGCGTTACCTTGAGGCGCTATTTATAGCATGCGAAAATCTGATCAAGGAATCATCCACCTCAGGTTTTGGTCAAAAGGAATTATATTTGCAGAAGAAATCTATTAACGAGCTGCGTGAATTAGAAGGGGCATACGATGCTGTGATTGTTTGTCTAGGTGCCAAAATGGTCATGCTTCCTGAACTTTCTGGAAAGCTTCCATTGAGAACTTGCAGAGGTGTAGTTGCTCATTTGCAGCTACCGGATGATATCAGCAATACATACCCAGAGCTTGGGCCTTCTATACTGTCAGATGCATGGCTTGCCATACAGGGTCCTAGGAGTTTATATATGGGTTCAACATGGGAATGGCAATCAACTAATTCATCACCCGAAGTTTCAGAAGATGAAGGCTCAAGAGCGCTCGCAGAACTTTTACCAAAGGTGTCTGCCATTTATCCCTCCATAAAGGAGTGGAGTTTCACAAGAGCAATGGCTGGTCTAAGAGCAATGCCACCACTTACTCCTCATGGATCTCTTCCTCTTTTAGGTTGTATAGACGAGATTGTAAGACATAACAGCTCTTGCAAGTATTGGTTATTTGGAGGGCTTGGTTCAAGGGGATTGTTGTACCATGGTTGGCTTGGGAAATTAACAGCGCAGGCTGTGCTTTCATGTAGTGAAGAACTTATTCCATCTGAACTAACCTCTTGGAAGAAAATAATGAATAGTTAA

Protein sequence

MLSLSQGSILQSAAMLSALSLSQRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCIDIYDEAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMDKCQNMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPLNTAFYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYDAVIVCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGPRSLYMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPPLTPHGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPSELTSWKKIMNS
Homology
BLAST of Sgr017514 vs. NCBI nr
Match: XP_004146732.1 (uncharacterized protein LOC101203097 isoform X1 [Cucumis sativus] >KGN65223.1 hypothetical protein Csa_019909 [Cucumis sativus])

HSP 1 Score: 768.1 bits (1982), Expect = 4.1e-218
Identity = 378/428 (88.32%), Postives = 401/428 (93.69%), Query Frame = 0

Query: 2   LSLSQGSILQSAAMLSAL-SLSQRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCIDIYD 61
           +S+S GS  Q+AA L AL SLS RPLRYAVLGAGFAGLSVTWHLLKLCP+DVP+ IDIYD
Sbjct: 4   VSVSGGSTFQAAAPLLALSSLSPRPLRYAVLGAGFAGLSVTWHLLKLCPKDVPIRIDIYD 63

Query: 62  EAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMDKCQ 121
           EAGIGGGASGVAGGLLHPYSPKVKLLWKG+ECWAECLKLLSVAESALH KELD EMD+CQ
Sbjct: 64  EAGIGGGASGVAGGLLHPYSPKVKLLWKGAECWAECLKLLSVAESALHPKELDSEMDECQ 123

Query: 122 NMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPLNTA 181
           NM+  IVLRRGILRPAIS KNL+VLKENAQNGLDSCRIETIDEDAARNL+P+LYVPLNTA
Sbjct: 124 NMNELIVLRRGILRPAISSKNLVVLKENAQNGLDSCRIETIDEDAARNLVPELYVPLNTA 183

Query: 182 FYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYDAVI 241
           FYMPQA NINSQRYLEALFIACENL KE+STSGFG KELYLQKKSI++L  LEG YDAVI
Sbjct: 184 FYMPQAVNINSQRYLEALFIACENLTKETSTSGFGHKELYLQKKSIDKLHGLEGTYDAVI 243

Query: 242 VCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGPRSL 301
           VCLGAKMVMLPEL+GKLPLRTCRGVVAH QL DDISN YP LGPSILSDAWLAI+GPRSL
Sbjct: 244 VCLGAKMVMLPELAGKLPLRTCRGVVAHFQLHDDISNAYPGLGPSILSDAWLAIRGPRSL 303

Query: 302 YMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPPLTP 361
           YMGSTWEW+STNSSPEVSE+E SRA+AELLPKVSAIYPSIKEWSF RA  GLRAMPPLTP
Sbjct: 304 YMGSTWEWKSTNSSPEVSEEESSRAVAELLPKVSAIYPSIKEWSFIRARGGLRAMPPLTP 363

Query: 362 HGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPSELT 421
           HGSLPL+GCIDEIVRHNSSCKYW+FGGLGSRGLLYHGWLGKLTAQAVLSC++ELIPSELT
Sbjct: 364 HGSLPLMGCIDEIVRHNSSCKYWVFGGLGSRGLLYHGWLGKLTAQAVLSCNDELIPSELT 423

Query: 422 SWKKIMNS 429
           SWKKIMNS
Sbjct: 424 SWKKIMNS 431

BLAST of Sgr017514 vs. NCBI nr
Match: TYK03632.1 (putative thiamine biosynthesis oxidoreductase ThiO [Cucumis melo var. makuwa])

HSP 1 Score: 766.9 bits (1979), Expect = 9.1e-218
Identity = 377/429 (87.88%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MLSLSQGSILQSAAMLSAL-SLSQRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCIDIY 60
           M+SLS GS  Q+AA L AL SLS RPLRYAVLGAGFAGLSVTWHLLKLCP+DVP+ ID+Y
Sbjct: 1   MVSLSGGSTFQAAAPLLALSSLSPRPLRYAVLGAGFAGLSVTWHLLKLCPKDVPIRIDLY 60

Query: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMDKC 120
           DEAGIGGGASGVAGGLLHPYSPKVKLLWKG+ECWAECLKLLSVAESALH KELD EMD+C
Sbjct: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGAECWAECLKLLSVAESALHPKELDSEMDEC 120

Query: 121 QNMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPLNT 180
           QNM+  IVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDE +AR+L+P+L VPLNT
Sbjct: 121 QNMNELIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEVSARDLVPELCVPLNT 180

Query: 181 AFYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYDAV 240
           AFYMPQA NINSQRYLEALFIACENL KE+STSGFG KELYLQKKSIN+LRELEG YDAV
Sbjct: 181 AFYMPQAVNINSQRYLEALFIACENLTKETSTSGFGHKELYLQKKSINKLRELEGTYDAV 240

Query: 241 IVCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGPRS 300
           IVCLGAKMVMLPEL+GKLPLRTCRGVVAH QL DDISN YP LGPSILSDAWLAI+GPRS
Sbjct: 241 IVCLGAKMVMLPELTGKLPLRTCRGVVAHFQLHDDISNAYPGLGPSILSDAWLAIRGPRS 300

Query: 301 LYMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPPLT 360
           LYMGSTWEW+STNSSPEVSE+E SRA+AELLPKVSAIYPSIKEWSF RA  GLRAMPPL 
Sbjct: 301 LYMGSTWEWKSTNSSPEVSEEESSRAVAELLPKVSAIYPSIKEWSFIRARGGLRAMPPLN 360

Query: 361 PHGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPSEL 420
           PHGSLPL+GCIDEI+RHNSSCKYW+FGGLGSRGLLYHGWLGKLTAQAVL+C++ELIPSEL
Sbjct: 361 PHGSLPLMGCIDEIIRHNSSCKYWVFGGLGSRGLLYHGWLGKLTAQAVLTCNDELIPSEL 420

Query: 421 TSWKKIMNS 429
           TSWKKIMNS
Sbjct: 421 TSWKKIMNS 429

BLAST of Sgr017514 vs. NCBI nr
Match: XP_008443892.1 (PREDICTED: uncharacterized protein LOC103487376 [Cucumis melo] >KAA0050029.1 putative thiamine biosynthesis oxidoreductase ThiO [Cucumis melo var. makuwa])

HSP 1 Score: 765.4 bits (1975), Expect = 2.6e-217
Identity = 376/429 (87.65%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MLSLSQGSILQSAAMLSAL-SLSQRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCIDIY 60
           M+SLS GS  Q+AA L AL SLS RPLRYAVLGAGFAGLSVTWHLLKLCP+DVP+ ID+Y
Sbjct: 1   MVSLSGGSTFQAAAPLLALSSLSPRPLRYAVLGAGFAGLSVTWHLLKLCPKDVPIRIDLY 60

Query: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMDKC 120
           DEAGIGGGASGVAGGLLHPYSPKVKLLWKG+ECWAECLKLLSVAESALH KELD EMD+C
Sbjct: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGAECWAECLKLLSVAESALHPKELDSEMDEC 120

Query: 121 QNMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPLNT 180
           QNM+  IVLRRGILRPAISLKNLMVLKENA+NGLDSCRIETIDE +AR+L+P+L VPLNT
Sbjct: 121 QNMNELIVLRRGILRPAISLKNLMVLKENARNGLDSCRIETIDEVSARDLVPELCVPLNT 180

Query: 181 AFYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYDAV 240
           AFYMPQA NINSQRYLEALFIACENL KE+STSGFG KELYLQKKSIN+LRELEG YDAV
Sbjct: 181 AFYMPQAVNINSQRYLEALFIACENLTKETSTSGFGHKELYLQKKSINKLRELEGTYDAV 240

Query: 241 IVCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGPRS 300
           IVCLGAKMVMLPEL+GKLPLRTCRGVVAH QL DDISN YP LGPSILSDAWLAI+GPRS
Sbjct: 241 IVCLGAKMVMLPELTGKLPLRTCRGVVAHFQLHDDISNAYPGLGPSILSDAWLAIRGPRS 300

Query: 301 LYMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPPLT 360
           LYMGSTWEW+STNSSPEVSE+E SRA+AELLPKVSAIYPSIKEWSF RA  GLRAMPPL 
Sbjct: 301 LYMGSTWEWKSTNSSPEVSEEESSRAVAELLPKVSAIYPSIKEWSFIRARGGLRAMPPLN 360

Query: 361 PHGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPSEL 420
           PHGSLPL+GCIDEI+RHNSSCKYW+FGGLGSRGLLYHGWLGKLTAQAVL+C++ELIPSEL
Sbjct: 361 PHGSLPLMGCIDEIIRHNSSCKYWVFGGLGSRGLLYHGWLGKLTAQAVLTCNDELIPSEL 420

Query: 421 TSWKKIMNS 429
           TSWKKIMNS
Sbjct: 421 TSWKKIMNS 429

BLAST of Sgr017514 vs. NCBI nr
Match: XP_022135024.1 (uncharacterized protein LOC111007124 isoform X1 [Momordica charantia])

HSP 1 Score: 763.8 bits (1971), Expect = 7.7e-217
Identity = 377/431 (87.47%), Postives = 398/431 (92.34%), Query Frame = 0

Query: 1   MLSLSQGSILQSAAMLSALSLS---QRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCID 60
           M+   +GS LQSAA   +L+LS   +R LRYAVLGAGFAGLSVTW+LLKLCP+DVPL ID
Sbjct: 1   MVCFCEGSTLQSAAAAPSLALSSLPRRSLRYAVLGAGFAGLSVTWNLLKLCPKDVPLRID 60

Query: 61  IYDEAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMD 120
           IYDE GIGGGASGVAGGLLHPYSPKVKLLWKG+ECWA+CL+LLSVAES LH KELD EMD
Sbjct: 61  IYDEVGIGGGASGVAGGLLHPYSPKVKLLWKGAECWADCLRLLSVAESTLHSKELDSEMD 120

Query: 121 KCQNMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPL 180
            CQNMH F+VLRRGILRPAISLKNL VLKENAQNGLDSCRIETIDED ARNL+PQL+VPL
Sbjct: 121 GCQNMHEFVVLRRGILRPAISLKNLTVLKENAQNGLDSCRIETIDEDDARNLVPQLHVPL 180

Query: 181 NTAFYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYD 240
           NTAFYMPQA NINS+RYLEALF ACENL KESSTSG G KELYLQK SIN+LREL GAYD
Sbjct: 181 NTAFYMPQAVNINSRRYLEALFTACENLTKESSTSGHGPKELYLQKDSINKLRELGGAYD 240

Query: 241 AVIVCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGP 300
           AVIVCLGAKMVMLPELSGKLPLRTCRGVVAH+QLPDDISNTYPELGPSILSDAWLAIQGP
Sbjct: 241 AVIVCLGAKMVMLPELSGKLPLRTCRGVVAHMQLPDDISNTYPELGPSILSDAWLAIQGP 300

Query: 301 RSLYMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPP 360
           RSLYMGSTWEWQSTNSSPEVSEDEG R LAELLPKV+AIYPSIKEWSFT A AGLRAMPP
Sbjct: 301 RSLYMGSTWEWQSTNSSPEVSEDEGLRTLAELLPKVAAIYPSIKEWSFTGARAGLRAMPP 360

Query: 361 LTPHGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPS 420
           LTP GSLPL+GCIDEIVRHNSSCKYWL GGLGSRGLLYHGWLGKLTAQAVLSC+E+LIPS
Sbjct: 361 LTPPGSLPLMGCIDEIVRHNSSCKYWLLGGLGSRGLLYHGWLGKLTAQAVLSCNEKLIPS 420

Query: 421 ELTSWKKIMNS 429
           ELTSWKK+MNS
Sbjct: 421 ELTSWKKMMNS 431

BLAST of Sgr017514 vs. NCBI nr
Match: XP_038879763.1 (uncharacterized protein LOC120071512 [Benincasa hispida])

HSP 1 Score: 750.4 bits (1936), Expect = 8.8e-213
Identity = 374/430 (86.98%), Postives = 396/430 (92.09%), Query Frame = 0

Query: 1   MLSLSQGSILQSAAMLSAL-SLSQRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCIDIY 60
           M+ +SQGS  Q AA L AL SLS  P RYAVLGAGFAGLSVTWHLLKLCP+DVPL IDIY
Sbjct: 1   MVCVSQGSTFQPAAPLLALSSLSALPRRYAVLGAGFAGLSVTWHLLKLCPKDVPLHIDIY 60

Query: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMDKC 120
           D  GIGGGASGVAGGLLHPYSPKVKLLW+G+ECWAECLKLLSVAESA+H KELD E D+C
Sbjct: 61  DGVGIGGGASGVAGGLLHPYSPKVKLLWEGAECWAECLKLLSVAESAVHPKELDSERDEC 120

Query: 121 QNMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPLNT 180
           QNM+ FIVLRRGILRPAISLKNLMVLKENAQN LDSC IETIDEDAARNL+P+L VPLNT
Sbjct: 121 QNMNEFIVLRRGILRPAISLKNLMVLKENAQNSLDSCMIETIDEDAARNLVPKLNVPLNT 180

Query: 181 AFYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYDAV 240
           AFYMPQA NINSQRYLEALFIACENL +E+STSGF  KELYL+KKSINELRELEG YDAV
Sbjct: 181 AFYMPQAVNINSQRYLEALFIACENLTRETSTSGFSHKELYLKKKSINELRELEGTYDAV 240

Query: 241 IVCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDI--SNTYPELGPSILSDAWLAIQGP 300
           IVCLGAKMVMLPEL+GKLPLRTCRGVVAH QL +DI  SN YPELGPSILSDAWLAIQGP
Sbjct: 241 IVCLGAKMVMLPELTGKLPLRTCRGVVAHFQLQNDISSSNAYPELGPSILSDAWLAIQGP 300

Query: 301 RSLYMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPP 360
           RSL MGSTWEW+STNSSPEVSE+E SRALAELLPKVSAIYPSIKEWSF +A AGLRAMPP
Sbjct: 301 RSLCMGSTWEWKSTNSSPEVSEEESSRALAELLPKVSAIYPSIKEWSFIKARAGLRAMPP 360

Query: 361 LTPHGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPS 420
           LTPHGSLPL+GCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSC+EELIPS
Sbjct: 361 LTPHGSLPLMGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCNEELIPS 420

Query: 421 ELTSWKKIMN 428
           ELTSWKKI++
Sbjct: 421 ELTSWKKIIS 430

BLAST of Sgr017514 vs. ExPASy TrEMBL
Match: A0A0A0LTV2 (DAO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G267190 PE=4 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.0e-218
Identity = 378/428 (88.32%), Postives = 401/428 (93.69%), Query Frame = 0

Query: 2   LSLSQGSILQSAAMLSAL-SLSQRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCIDIYD 61
           +S+S GS  Q+AA L AL SLS RPLRYAVLGAGFAGLSVTWHLLKLCP+DVP+ IDIYD
Sbjct: 4   VSVSGGSTFQAAAPLLALSSLSPRPLRYAVLGAGFAGLSVTWHLLKLCPKDVPIRIDIYD 63

Query: 62  EAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMDKCQ 121
           EAGIGGGASGVAGGLLHPYSPKVKLLWKG+ECWAECLKLLSVAESALH KELD EMD+CQ
Sbjct: 64  EAGIGGGASGVAGGLLHPYSPKVKLLWKGAECWAECLKLLSVAESALHPKELDSEMDECQ 123

Query: 122 NMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPLNTA 181
           NM+  IVLRRGILRPAIS KNL+VLKENAQNGLDSCRIETIDEDAARNL+P+LYVPLNTA
Sbjct: 124 NMNELIVLRRGILRPAISSKNLVVLKENAQNGLDSCRIETIDEDAARNLVPELYVPLNTA 183

Query: 182 FYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYDAVI 241
           FYMPQA NINSQRYLEALFIACENL KE+STSGFG KELYLQKKSI++L  LEG YDAVI
Sbjct: 184 FYMPQAVNINSQRYLEALFIACENLTKETSTSGFGHKELYLQKKSIDKLHGLEGTYDAVI 243

Query: 242 VCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGPRSL 301
           VCLGAKMVMLPEL+GKLPLRTCRGVVAH QL DDISN YP LGPSILSDAWLAI+GPRSL
Sbjct: 244 VCLGAKMVMLPELAGKLPLRTCRGVVAHFQLHDDISNAYPGLGPSILSDAWLAIRGPRSL 303

Query: 302 YMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPPLTP 361
           YMGSTWEW+STNSSPEVSE+E SRA+AELLPKVSAIYPSIKEWSF RA  GLRAMPPLTP
Sbjct: 304 YMGSTWEWKSTNSSPEVSEEESSRAVAELLPKVSAIYPSIKEWSFIRARGGLRAMPPLTP 363

Query: 362 HGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPSELT 421
           HGSLPL+GCIDEIVRHNSSCKYW+FGGLGSRGLLYHGWLGKLTAQAVLSC++ELIPSELT
Sbjct: 364 HGSLPLMGCIDEIVRHNSSCKYWVFGGLGSRGLLYHGWLGKLTAQAVLSCNDELIPSELT 423

Query: 422 SWKKIMNS 429
           SWKKIMNS
Sbjct: 424 SWKKIMNS 431

BLAST of Sgr017514 vs. ExPASy TrEMBL
Match: A0A5D3BXR2 (Putative thiamine biosynthesis oxidoreductase ThiO OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00600 PE=4 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 4.4e-218
Identity = 377/429 (87.88%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MLSLSQGSILQSAAMLSAL-SLSQRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCIDIY 60
           M+SLS GS  Q+AA L AL SLS RPLRYAVLGAGFAGLSVTWHLLKLCP+DVP+ ID+Y
Sbjct: 1   MVSLSGGSTFQAAAPLLALSSLSPRPLRYAVLGAGFAGLSVTWHLLKLCPKDVPIRIDLY 60

Query: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMDKC 120
           DEAGIGGGASGVAGGLLHPYSPKVKLLWKG+ECWAECLKLLSVAESALH KELD EMD+C
Sbjct: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGAECWAECLKLLSVAESALHPKELDSEMDEC 120

Query: 121 QNMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPLNT 180
           QNM+  IVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDE +AR+L+P+L VPLNT
Sbjct: 121 QNMNELIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEVSARDLVPELCVPLNT 180

Query: 181 AFYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYDAV 240
           AFYMPQA NINSQRYLEALFIACENL KE+STSGFG KELYLQKKSIN+LRELEG YDAV
Sbjct: 181 AFYMPQAVNINSQRYLEALFIACENLTKETSTSGFGHKELYLQKKSINKLRELEGTYDAV 240

Query: 241 IVCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGPRS 300
           IVCLGAKMVMLPEL+GKLPLRTCRGVVAH QL DDISN YP LGPSILSDAWLAI+GPRS
Sbjct: 241 IVCLGAKMVMLPELTGKLPLRTCRGVVAHFQLHDDISNAYPGLGPSILSDAWLAIRGPRS 300

Query: 301 LYMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPPLT 360
           LYMGSTWEW+STNSSPEVSE+E SRA+AELLPKVSAIYPSIKEWSF RA  GLRAMPPL 
Sbjct: 301 LYMGSTWEWKSTNSSPEVSEEESSRAVAELLPKVSAIYPSIKEWSFIRARGGLRAMPPLN 360

Query: 361 PHGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPSEL 420
           PHGSLPL+GCIDEI+RHNSSCKYW+FGGLGSRGLLYHGWLGKLTAQAVL+C++ELIPSEL
Sbjct: 361 PHGSLPLMGCIDEIIRHNSSCKYWVFGGLGSRGLLYHGWLGKLTAQAVLTCNDELIPSEL 420

Query: 421 TSWKKIMNS 429
           TSWKKIMNS
Sbjct: 421 TSWKKIMNS 429

BLAST of Sgr017514 vs. ExPASy TrEMBL
Match: A0A5A7U967 (Putative thiamine biosynthesis oxidoreductase ThiO OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00030 PE=4 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.3e-217
Identity = 376/429 (87.65%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MLSLSQGSILQSAAMLSAL-SLSQRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCIDIY 60
           M+SLS GS  Q+AA L AL SLS RPLRYAVLGAGFAGLSVTWHLLKLCP+DVP+ ID+Y
Sbjct: 1   MVSLSGGSTFQAAAPLLALSSLSPRPLRYAVLGAGFAGLSVTWHLLKLCPKDVPIRIDLY 60

Query: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMDKC 120
           DEAGIGGGASGVAGGLLHPYSPKVKLLWKG+ECWAECLKLLSVAESALH KELD EMD+C
Sbjct: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGAECWAECLKLLSVAESALHPKELDSEMDEC 120

Query: 121 QNMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPLNT 180
           QNM+  IVLRRGILRPAISLKNLMVLKENA+NGLDSCRIETIDE +AR+L+P+L VPLNT
Sbjct: 121 QNMNELIVLRRGILRPAISLKNLMVLKENARNGLDSCRIETIDEVSARDLVPELCVPLNT 180

Query: 181 AFYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYDAV 240
           AFYMPQA NINSQRYLEALFIACENL KE+STSGFG KELYLQKKSIN+LRELEG YDAV
Sbjct: 181 AFYMPQAVNINSQRYLEALFIACENLTKETSTSGFGHKELYLQKKSINKLRELEGTYDAV 240

Query: 241 IVCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGPRS 300
           IVCLGAKMVMLPEL+GKLPLRTCRGVVAH QL DDISN YP LGPSILSDAWLAI+GPRS
Sbjct: 241 IVCLGAKMVMLPELTGKLPLRTCRGVVAHFQLHDDISNAYPGLGPSILSDAWLAIRGPRS 300

Query: 301 LYMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPPLT 360
           LYMGSTWEW+STNSSPEVSE+E SRA+AELLPKVSAIYPSIKEWSF RA  GLRAMPPL 
Sbjct: 301 LYMGSTWEWKSTNSSPEVSEEESSRAVAELLPKVSAIYPSIKEWSFIRARGGLRAMPPLN 360

Query: 361 PHGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPSEL 420
           PHGSLPL+GCIDEI+RHNSSCKYW+FGGLGSRGLLYHGWLGKLTAQAVL+C++ELIPSEL
Sbjct: 361 PHGSLPLMGCIDEIIRHNSSCKYWVFGGLGSRGLLYHGWLGKLTAQAVLTCNDELIPSEL 420

Query: 421 TSWKKIMNS 429
           TSWKKIMNS
Sbjct: 421 TSWKKIMNS 429

BLAST of Sgr017514 vs. ExPASy TrEMBL
Match: A0A1S3B962 (uncharacterized protein LOC103487376 OS=Cucumis melo OX=3656 GN=LOC103487376 PE=4 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.3e-217
Identity = 376/429 (87.65%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MLSLSQGSILQSAAMLSAL-SLSQRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCIDIY 60
           M+SLS GS  Q+AA L AL SLS RPLRYAVLGAGFAGLSVTWHLLKLCP+DVP+ ID+Y
Sbjct: 1   MVSLSGGSTFQAAAPLLALSSLSPRPLRYAVLGAGFAGLSVTWHLLKLCPKDVPIRIDLY 60

Query: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMDKC 120
           DEAGIGGGASGVAGGLLHPYSPKVKLLWKG+ECWAECLKLLSVAESALH KELD EMD+C
Sbjct: 61  DEAGIGGGASGVAGGLLHPYSPKVKLLWKGAECWAECLKLLSVAESALHPKELDSEMDEC 120

Query: 121 QNMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPLNT 180
           QNM+  IVLRRGILRPAISLKNLMVLKENA+NGLDSCRIETIDE +AR+L+P+L VPLNT
Sbjct: 121 QNMNELIVLRRGILRPAISLKNLMVLKENARNGLDSCRIETIDEVSARDLVPELCVPLNT 180

Query: 181 AFYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYDAV 240
           AFYMPQA NINSQRYLEALFIACENL KE+STSGFG KELYLQKKSIN+LRELEG YDAV
Sbjct: 181 AFYMPQAVNINSQRYLEALFIACENLTKETSTSGFGHKELYLQKKSINKLRELEGTYDAV 240

Query: 241 IVCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGPRS 300
           IVCLGAKMVMLPEL+GKLPLRTCRGVVAH QL DDISN YP LGPSILSDAWLAI+GPRS
Sbjct: 241 IVCLGAKMVMLPELTGKLPLRTCRGVVAHFQLHDDISNAYPGLGPSILSDAWLAIRGPRS 300

Query: 301 LYMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPPLT 360
           LYMGSTWEW+STNSSPEVSE+E SRA+AELLPKVSAIYPSIKEWSF RA  GLRAMPPL 
Sbjct: 301 LYMGSTWEWKSTNSSPEVSEEESSRAVAELLPKVSAIYPSIKEWSFIRARGGLRAMPPLN 360

Query: 361 PHGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPSEL 420
           PHGSLPL+GCIDEI+RHNSSCKYW+FGGLGSRGLLYHGWLGKLTAQAVL+C++ELIPSEL
Sbjct: 361 PHGSLPLMGCIDEIIRHNSSCKYWVFGGLGSRGLLYHGWLGKLTAQAVLTCNDELIPSEL 420

Query: 421 TSWKKIMNS 429
           TSWKKIMNS
Sbjct: 421 TSWKKIMNS 429

BLAST of Sgr017514 vs. ExPASy TrEMBL
Match: A0A6J1C0B6 (uncharacterized protein LOC111007124 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007124 PE=4 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 3.7e-217
Identity = 377/431 (87.47%), Postives = 398/431 (92.34%), Query Frame = 0

Query: 1   MLSLSQGSILQSAAMLSALSLS---QRPLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCID 60
           M+   +GS LQSAA   +L+LS   +R LRYAVLGAGFAGLSVTW+LLKLCP+DVPL ID
Sbjct: 1   MVCFCEGSTLQSAAAAPSLALSSLPRRSLRYAVLGAGFAGLSVTWNLLKLCPKDVPLRID 60

Query: 61  IYDEAGIGGGASGVAGGLLHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFEMD 120
           IYDE GIGGGASGVAGGLLHPYSPKVKLLWKG+ECWA+CL+LLSVAES LH KELD EMD
Sbjct: 61  IYDEVGIGGGASGVAGGLLHPYSPKVKLLWKGAECWADCLRLLSVAESTLHSKELDSEMD 120

Query: 121 KCQNMHGFIVLRRGILRPAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPL 180
            CQNMH F+VLRRGILRPAISLKNL VLKENAQNGLDSCRIETIDED ARNL+PQL+VPL
Sbjct: 121 GCQNMHEFVVLRRGILRPAISLKNLTVLKENAQNGLDSCRIETIDEDDARNLVPQLHVPL 180

Query: 181 NTAFYMPQAGNINSQRYLEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYD 240
           NTAFYMPQA NINS+RYLEALF ACENL KESSTSG G KELYLQK SIN+LREL GAYD
Sbjct: 181 NTAFYMPQAVNINSRRYLEALFTACENLTKESSTSGHGPKELYLQKDSINKLRELGGAYD 240

Query: 241 AVIVCLGAKMVMLPELSGKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGP 300
           AVIVCLGAKMVMLPELSGKLPLRTCRGVVAH+QLPDDISNTYPELGPSILSDAWLAIQGP
Sbjct: 241 AVIVCLGAKMVMLPELSGKLPLRTCRGVVAHMQLPDDISNTYPELGPSILSDAWLAIQGP 300

Query: 301 RSLYMGSTWEWQSTNSSPEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPP 360
           RSLYMGSTWEWQSTNSSPEVSEDEG R LAELLPKV+AIYPSIKEWSFT A AGLRAMPP
Sbjct: 301 RSLYMGSTWEWQSTNSSPEVSEDEGLRTLAELLPKVAAIYPSIKEWSFTGARAGLRAMPP 360

Query: 361 LTPHGSLPLLGCIDEIVRHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPS 420
           LTP GSLPL+GCIDEIVRHNSSCKYWL GGLGSRGLLYHGWLGKLTAQAVLSC+E+LIPS
Sbjct: 361 LTPPGSLPLMGCIDEIVRHNSSCKYWLLGGLGSRGLLYHGWLGKLTAQAVLSCNEKLIPS 420

Query: 421 ELTSWKKIMNS 429
           ELTSWKK+MNS
Sbjct: 421 ELTSWKKMMNS 431

BLAST of Sgr017514 vs. TAIR 10
Match: AT2G22650.1 (FAD-dependent oxidoreductase family protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.2e-151
Identity = 251/410 (61.22%), Postives = 319/410 (77.80%), Query Frame = 0

Query: 17  SALSLSQR-PLRYAVLGAGFAGLSVTWHLLKLCPRDVPLCIDIYDEAGIGGGASGVAGGL 76
           ++LSL  R PLRYA+LGAGFAG+SV WHLLK  P+++ L +D+YDE GIGGGASGV+GGL
Sbjct: 12  ASLSLPNRPPLRYAILGAGFAGISVAWHLLKESPKELRLSVDVYDEVGIGGGASGVSGGL 71

Query: 77  LHPYSPKVKLLWKGSECWAECLKLLSVAESALHFKELDFE-MDKCQNMHGFIVLRRGILR 136
           LHPYSPK KLLW G+ECW ECL+LL+VAE+A        E  D  ++   F+V RRGILR
Sbjct: 72  LHPYSPKGKLLWHGAECWRECLELLNVAETAASSSYQSTENRDFSESFGNFMVRRRGILR 131

Query: 137 PAISLKNLMVLKENAQNGLDSCRIETIDEDAARNLIPQLYVPLNTAFYMPQAGNINSQRY 196
           PA + K L ++ +NA+N L SC +ETID+D A+NL+P LY+PLN AFY P A N+N  RY
Sbjct: 132 PATNAKTLDLMSDNARNCLASCVVETIDKDGAQNLVPNLYLPLNCAFYFPGAMNVNPHRY 191

Query: 197 LEALFIACENLIKESSTSGFGQKELYLQKKSINELRELEGAYDAVIVCLGAKMVMLPELS 256
           L+ALF+AC++    S+ +  G+K + L  KSI ++ ELEG YDAV++CLG+K+  LP L+
Sbjct: 192 LQALFLACQS----SAKTSLGRKNITLVNKSITDVLELEGGYDAVVICLGSKVDFLPGLT 251

Query: 257 GKLPLRTCRGVVAHLQLPDDISNTYPELGPSILSDAWLAIQGPRSLYMGSTWEWQSTNSS 316
           GKLPLRTCRGV+ H+QL +    +YP+ GPSILSDAWLA+QGPR+L+MGSTWEWQS N S
Sbjct: 252 GKLPLRTCRGVITHMQLHESARGSYPDDGPSILSDAWLAVQGPRNLHMGSTWEWQSRNYS 311

Query: 317 PEVSEDEGSRALAELLPKVSAIYPSIKEWSFTRAMAGLRAMPPLTPHGSLPLLGCIDEIV 376
            +V  +E SRAL ELLPK SA+YP I++W F  A AGLRAMPP+T HGSLPLLGC+++++
Sbjct: 312 SDVPAEEASRALNELLPKASAVYPDIEKWEFAGARAGLRAMPPVTSHGSLPLLGCVNQLI 371

Query: 377 RHNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCSEELIPSELTSWKK 425
                C +W+F GLGSRGLLYH WLGKL ++AVLSC+EEL+PSELTSWKK
Sbjct: 372 GAGQFCNFWVFSGLGSRGLLYHAWLGKLMSKAVLSCNEELLPSELTSWKK 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146732.14.1e-21888.32uncharacterized protein LOC101203097 isoform X1 [Cucumis sativus] >KGN65223.1 hy... [more]
TYK03632.19.1e-21887.88putative thiamine biosynthesis oxidoreductase ThiO [Cucumis melo var. makuwa][more]
XP_008443892.12.6e-21787.65PREDICTED: uncharacterized protein LOC103487376 [Cucumis melo] >KAA0050029.1 put... [more]
XP_022135024.17.7e-21787.47uncharacterized protein LOC111007124 isoform X1 [Momordica charantia][more]
XP_038879763.18.8e-21386.98uncharacterized protein LOC120071512 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LTV22.0e-21888.32DAO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G267190 PE=4 SV... [more]
A0A5D3BXR24.4e-21887.88Putative thiamine biosynthesis oxidoreductase ThiO OS=Cucumis melo var. makuwa O... [more]
A0A5A7U9671.3e-21787.65Putative thiamine biosynthesis oxidoreductase ThiO OS=Cucumis melo var. makuwa O... [more]
A0A1S3B9621.3e-21787.65uncharacterized protein LOC103487376 OS=Cucumis melo OX=3656 GN=LOC103487376 PE=... [more]
A0A6J1C0B63.7e-21787.47uncharacterized protein LOC111007124 isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT2G22650.11.2e-15161.22FAD-dependent oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 29..407
e-value: 5.6E-26
score: 93.6
IPR006076FAD dependent oxidoreductasePFAMPF01266DAOcoord: 28..405
e-value: 4.6E-31
score: 108.7
NoneNo IPR availableGENE3D3.30.9.10coord: 129..356
e-value: 5.6E-26
score: 93.6
NoneNo IPR availablePANTHERPTHR13847SARCOSINE DEHYDROGENASE-RELATEDcoord: 19..422
NoneNo IPR availablePANTHERPTHR13847:SF261FAD-DEPENDENT OXIDOREDUCTASE FAMILY PROTEINcoord: 19..422
NoneNo IPR availableSUPERFAMILY51971Nucleotide-binding domaincoord: 25..409

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr017514.1Sgr017514.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016491 oxidoreductase activity