Sgr017295 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr017295
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionprotein MIZU-KUSSEI 1
Locationtig00153037: 562493 .. 563323 (+)
RNA-Seq ExpressionSgr017295
SyntenySgr017295
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAGCCAAGAGTTGACCACCCTGCAACGAACCAGAAGCTGCAGCGCCGCCATTAAAAGCACCAGAAAGATCATCCCTGCTTCCAGCTCCGGCCACCACCTCAGATCCTCCAGCTCAAACCTCCCGCCGGAGTATTCCGACGCGGGCAATCTTTCTGGCAAATTCCTGCTCCAGAAGAAGTCCGACCACCCTGCAGCTTCCACCCGCGGAAAATTCTCCTCCCTCCTCCACTCCCTTCTCAAGCTCCTCTCTTTTCCCACCCTTAATATTCCCACTTGCAAATGGCTCTCCATCCCCACCCACCTCTCCGTCAGCCCCTCTCTCCGCCGTAAAGTCACCGGAACTCTCTTCGGCCACCGCCGCGGGCACGTCACCTTCGCTGTCCAACTAGACCCCCGGTCCGAACCGGTTCTGTTGCTCGAGTTGGCAACGTCGACGTCCTCCCTCGTCAAGGAAATGTCCTCCGGTCTCGTCCGCATCGCCCTCGAGTCTGAAACTCCGGCAGACGACGGCCGGCCGAGGAGGCTGTTTCAGGAGCCGAGATGGACGATGTACTGCAACGGAAGGAAGTGCGGGTACGCGACGTCGCGCGCGTGCGGAGAGTTCGACTGGCACGTGCTGAACACAGTGCAGAGCGTGTCGGTCGGCGCCGGAGTGATTCCGGTGGTCGACGACGGCCGGAAAGGCAGTGGGACGGAGGGCGAGTTGCTGTACATGAGAGCGAGGTTCGAGCGAGTCGTAGGTAGCCGTGACTCGGAAGCTTTTTACATGATGAACCCGGACGGTAATGGAGGGCCTGAACTCAGTATTTTTCTACTCAGAATATAA

mRNA sequence

ATGAGAAGCCAAGAGTTGACCACCCTGCAACGAACCAGAAGCTGCAGCGCCGCCATTAAAAGCACCAGAAAGATCATCCCTGCTTCCAGCTCCGGCCACCACCTCAGATCCTCCAGCTCAAACCTCCCGCCGGAGTATTCCGACGCGGGCAATCTTTCTGGCAAATTCCTGCTCCAGAAGAAGTCCGACCACCCTGCAGCTTCCACCCGCGGAAAATTCTCCTCCCTCCTCCACTCCCTTCTCAAGCTCCTCTCTTTTCCCACCCTTAATATTCCCACTTGCAAATGGCTCTCCATCCCCACCCACCTCTCCGTCAGCCCCTCTCTCCGCCGTAAAGTCACCGGAACTCTCTTCGGCCACCGCCGCGGGCACGTCACCTTCGCTGTCCAACTAGACCCCCGGTCCGAACCGGTTCTGTTGCTCGAGTTGGCAACGTCGACGTCCTCCCTCGTCAAGGAAATGTCCTCCGGTCTCGTCCGCATCGCCCTCGAGTCTGAAACTCCGGCAGACGACGGCCGGCCGAGGAGGCTGTTTCAGGAGCCGAGATGGACGATGTACTGCAACGGAAGGAAGTGCGGGTACGCGACGTCGCGCGCGTGCGGAGAGTTCGACTGGCACGTGCTGAACACAGTGCAGAGCGTGTCGGTCGGCGCCGGAGTGATTCCGGTGGTCGACGACGGCCGGAAAGGCAGTGGGACGGAGGGCGAGTTGCTGTACATGAGAGCGAGGTTCGAGCGAGTCGTAGGTAGCCGTGACTCGGAAGCTTTTTACATGATGAACCCGGACGGTAATGGAGGGCCTGAACTCAGTATTTTTCTACTCAGAATATAA

Coding sequence (CDS)

ATGAGAAGCCAAGAGTTGACCACCCTGCAACGAACCAGAAGCTGCAGCGCCGCCATTAAAAGCACCAGAAAGATCATCCCTGCTTCCAGCTCCGGCCACCACCTCAGATCCTCCAGCTCAAACCTCCCGCCGGAGTATTCCGACGCGGGCAATCTTTCTGGCAAATTCCTGCTCCAGAAGAAGTCCGACCACCCTGCAGCTTCCACCCGCGGAAAATTCTCCTCCCTCCTCCACTCCCTTCTCAAGCTCCTCTCTTTTCCCACCCTTAATATTCCCACTTGCAAATGGCTCTCCATCCCCACCCACCTCTCCGTCAGCCCCTCTCTCCGCCGTAAAGTCACCGGAACTCTCTTCGGCCACCGCCGCGGGCACGTCACCTTCGCTGTCCAACTAGACCCCCGGTCCGAACCGGTTCTGTTGCTCGAGTTGGCAACGTCGACGTCCTCCCTCGTCAAGGAAATGTCCTCCGGTCTCGTCCGCATCGCCCTCGAGTCTGAAACTCCGGCAGACGACGGCCGGCCGAGGAGGCTGTTTCAGGAGCCGAGATGGACGATGTACTGCAACGGAAGGAAGTGCGGGTACGCGACGTCGCGCGCGTGCGGAGAGTTCGACTGGCACGTGCTGAACACAGTGCAGAGCGTGTCGGTCGGCGCCGGAGTGATTCCGGTGGTCGACGACGGCCGGAAAGGCAGTGGGACGGAGGGCGAGTTGCTGTACATGAGAGCGAGGTTCGAGCGAGTCGTAGGTAGCCGTGACTCGGAAGCTTTTTACATGATGAACCCGGACGGTAATGGAGGGCCTGAACTCAGTATTTTTCTACTCAGAATATAA

Protein sequence

MRSQELTTLQRTRSCSAAIKSTRKIIPASSSGHHLRSSSSNLPPEYSDAGNLSGKFLLQKKSDHPAASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSLRRKVTGTLFGHRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPADDGRPRRLFQEPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGSGTEGELLYMRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI
Homology
BLAST of Sgr017295 vs. NCBI nr
Match: XP_022924911.1 (protein MIZU-KUSSEI 1 [Cucurbita moschata])

HSP 1 Score: 414.1 bits (1063), Expect = 9.6e-112
Identity = 219/280 (78.21%), Postives = 237/280 (84.64%), Query Frame = 0

Query: 6   LTTLQRTRSCSAAIKSTRKIIPASSSGHHLRSSSSNLPPEYSDAGNLSGKFLLQKKSDHP 65
           +T LQRT+SCSAAI++T KIIP+S S HHLRSS        SDA N SGKFLLQK S H 
Sbjct: 1   MTGLQRTKSCSAAIQTTTKIIPSSISAHHLRSS--------SDADNFSGKFLLQKNSSHR 60

Query: 66  ------AASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSLRRKVTGTLFG 125
                 AAST GKFSSLLHSLLKLLS     IPTCKWLSIPTHLSVSPSL R+VTGTLFG
Sbjct: 61  CHRQHLAASTTGKFSSLLHSLLKLLS-----IPTCKWLSIPTHLSVSPSLGRRVTGTLFG 120

Query: 126 HRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPA--DDGRP-RR 185
            RRGHVTFA+QLDPRS+PVLLLE ATSTSSL+KEMSSGLVRIALES+ P   D  RP R+
Sbjct: 121 RRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRK 180

Query: 186 LFQEPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGSGTEGE 245
           L +EPRWTMYCNGRKCG A SR CGEF+W+VLNTVQSVSVGAGVIP+VDDGRKGSG+EGE
Sbjct: 181 LLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGE 240

Query: 246 LLYMRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
           LLYMRA+FERV+GSRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 LLYMRAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of Sgr017295 vs. NCBI nr
Match: KAG7027634.1 (Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 413.3 bits (1061), Expect = 1.6e-111
Identity = 219/280 (78.21%), Postives = 236/280 (84.29%), Query Frame = 0

Query: 6   LTTLQRTRSCSAAIKSTRKIIPASSSGHHLRSSSSNLPPEYSDAGNLSGKFLLQKKSDHP 65
           +T LQRT+SCSAAIK+T KIIP+S S HHLRSS        SDA N SGKFLLQK S H 
Sbjct: 1   MTGLQRTKSCSAAIKTTTKIIPSSISAHHLRSS--------SDADNFSGKFLLQKNSSHR 60

Query: 66  ------AASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSLRRKVTGTLFG 125
                 AAST GKFS LLHSLLKLLS     IPTCKWLSIPTHLSVSPSL R+VTGTLFG
Sbjct: 61  CHRQHLAASTTGKFSFLLHSLLKLLS-----IPTCKWLSIPTHLSVSPSLGRRVTGTLFG 120

Query: 126 HRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPA--DDGRP-RR 185
            RRGHVTFA+QLDPRS+PVLLLE ATSTSSL+KEMSSGLVRIALES+ P   D  RP R+
Sbjct: 121 RRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRK 180

Query: 186 LFQEPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGSGTEGE 245
           L +EPRWTMYCNGRKCG A SR CGEF+W+VLNTVQSVSVGAGVIP+VDDGRKGSG+EGE
Sbjct: 181 LLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGE 240

Query: 246 LLYMRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
           LLYMRA+FERV+GSRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 LLYMRAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of Sgr017295 vs. NCBI nr
Match: XP_023518638.1 (protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 411.4 bits (1056), Expect = 6.2e-111
Identity = 220/280 (78.57%), Postives = 236/280 (84.29%), Query Frame = 0

Query: 6   LTTLQRTRSCSAAIKSTRKIIPASSSGHHLRSSSSNLPPEYSDAGNLSGKFLLQKKSDHP 65
           +T LQRTRSCSAAIK+T KIIP+S S HHLRSS        SDA N SGKFLLQK S H 
Sbjct: 1   MTGLQRTRSCSAAIKTTTKIIPSSISAHHLRSS--------SDADNFSGKFLLQKNSSHR 60

Query: 66  ------AASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSLRRKVTGTLFG 125
                 AAST GKFSSLLHSLLKLLS     IPTCKWL IPTHLSVSPSL R+VTGTLFG
Sbjct: 61  CHRQHLAASTTGKFSSLLHSLLKLLS-----IPTCKWLPIPTHLSVSPSLGRRVTGTLFG 120

Query: 126 HRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPA--DDGRP-RR 185
            RRGHVTFA+QLDPRS+PVLLLE ATSTSSL+KEMSSGLVRIALES++P   D  RP R+
Sbjct: 121 RRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDSPTTYDGNRPIRK 180

Query: 186 LFQEPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGSGTEGE 245
           L +EPRWTMY NGRKCG A SR CGEF+W+VLNTVQSVSVGAGVIP+VDDGRKGSG+EGE
Sbjct: 181 LLEEPRWTMYYNGRKCGQAISRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGE 240

Query: 246 LLYMRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
           LLYMRA+FERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 LLYMRAKFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of Sgr017295 vs. NCBI nr
Match: XP_038877466.1 (protein MIZU-KUSSEI 1-like [Benincasa hispida])

HSP 1 Score: 406.8 bits (1044), Expect = 1.5e-109
Identity = 218/283 (77.03%), Postives = 238/283 (84.10%), Query Frame = 0

Query: 6   LTTLQRTRSCSAAIKSTRKIIPASSSG-----HHLRS-SSSNLPPEYSDAGNLSGKFLLQ 65
           +T LQRTRSC AAIK++ K+IP+S S      HHLRS SSSNLPPEYS+  N SG+FLL 
Sbjct: 1   MTGLQRTRSCMAAIKTSTKLIPSSISDHQHRHHHLRSFSSSNLPPEYSNPDNFSGRFLLH 60

Query: 66  KKSDHPA----ASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSL-RRKVT 125
           K S H      A+   KFS+LLHSLLKLLS     +PTCKW SIPTHLSVS SL RR VT
Sbjct: 61  KNSGHHGHHHQAAAGSKFSTLLHSLLKLLS-----VPTCKWFSIPTHLSVSSSLGRRVVT 120

Query: 126 GTLFGHRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPA-DDGR 185
           GTLFG RRGHVTFAVQLDPRSEPVLLLELATSTS LVKEMSSGLVRIALESE PA ++ R
Sbjct: 121 GTLFGRRRGHVTFAVQLDPRSEPVLLLELATSTSFLVKEMSSGLVRIALESEAPAYNESR 180

Query: 186 PRRLFQEPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGSGT 245
           PR+LF+EPRWTMYCNGRKCG++T R CGEF+W+VLNTVQ VSVGAGVIPVVDDGRKGS +
Sbjct: 181 PRKLFEEPRWTMYCNGRKCGHSTLRVCGEFEWYVLNTVQRVSVGAGVIPVVDDGRKGSRS 240

Query: 246 EGELLYMRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
           EGELLYMRA +ERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 EGELLYMRANYERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 278

BLAST of Sgr017295 vs. NCBI nr
Match: XP_022966431.1 (protein MIZU-KUSSEI 1-like [Cucurbita maxima])

HSP 1 Score: 404.4 bits (1038), Expect = 7.6e-109
Identity = 218/280 (77.86%), Postives = 233/280 (83.21%), Query Frame = 0

Query: 6   LTTLQRTRSCSAAIKSTRKIIPASSSGHHLRSSSSNLPPEYSDAGNLSGKFLLQKKSDHP 65
           +T LQRT+SCSAAIK+T KIIP+S SGHHLRSS        SDA N S KFLLQK S H 
Sbjct: 1   MTGLQRTKSCSAAIKTTTKIIPSSISGHHLRSS--------SDADNFSAKFLLQKNSSHR 60

Query: 66  ------AASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSLRRKVTGTLFG 125
                 AAST GKFSSLLHSLLKL+S     IPTCKWLSIPTHLSVSPSL R+VTGTLFG
Sbjct: 61  CHRQHLAASTTGKFSSLLHSLLKLIS-----IPTCKWLSIPTHLSVSPSLGRRVTGTLFG 120

Query: 126 HRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESE--TPADDGRP-RR 185
            RRGHVTFAVQLDPRSEPVLLLE ATSTSSL+KEMSSGLVRIALES+  T  D  R  R+
Sbjct: 121 RRRGHVTFAVQLDPRSEPVLLLEFATSTSSLIKEMSSGLVRIALESDALTTYDGNRSIRK 180

Query: 186 LFQEPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGSGTEGE 245
           L +EPRWTMYCNGRKCG A SR CGEF+W+VLNTVQSVSVGAGVIP+VDDG  GS +EGE
Sbjct: 181 LLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGHMGSRSEGE 240

Query: 246 LLYMRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
           LLYMRA+FERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 LLYMRAKFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of Sgr017295 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 9.8e-67
Identity = 169/310 (54.52%), Postives = 197/310 (63.55%), Query Frame = 0

Query: 4   QELTTLQRTRSCSAAIKSTRKIIPASSSGH--HLRS-SSSNLPPEYSDAGNLSGKFLL-- 63
           QEL TLQR+ S      ++RKI P +S     H+RS SSS L P   +       FL+  
Sbjct: 5   QEL-TLQRSFS-----YNSRKINPVTSPARSSHVRSPSSSALIPSIPE----HELFLVPC 64

Query: 64  ----------QKKSDHPAASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSV--- 123
                        + H       KFS LL S + +     +NIP CK LS+P+  S    
Sbjct: 65  RRCSYVPLSSSSSASHNIGKFHLKFSLLLRSFINI-----INIPACKMLSLPSPPSSSSS 124

Query: 124 ------------SPSLRRKVTGTLFGHRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKE 183
                       S SL R+VTGTL+GH+RGHVTF+VQ + RS+PVLLL+LA ST++LVKE
Sbjct: 125 VSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKE 184

Query: 184 MSSGLVRIALESETPADDGRPRRLFQEPRWTMYCNGRKCGYATSR--ACGEFDWHVLNTV 243
           MSSGLVRIALE E     G   +LFQEP+WTMYCNGRKCGYA SR  AC + DW VLNTV
Sbjct: 185 MSSGLVRIALECEKRHRSG--TKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTV 244

Query: 244 QSVSVGAGVIP---VVDD-GRKGSGTE-GELLYMRARFERVVGSRDSEAFYMMNPDGNGG 277
             V+VGAGVIP    +DD    GSGTE GELLYMR +FERVVGSRDSEAFYMMNPD NGG
Sbjct: 245 SRVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGG 297

BLAST of Sgr017295 vs. ExPASy TrEMBL
Match: A0A6J1EAS0 (protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111432316 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 4.7e-112
Identity = 219/280 (78.21%), Postives = 237/280 (84.64%), Query Frame = 0

Query: 6   LTTLQRTRSCSAAIKSTRKIIPASSSGHHLRSSSSNLPPEYSDAGNLSGKFLLQKKSDHP 65
           +T LQRT+SCSAAI++T KIIP+S S HHLRSS        SDA N SGKFLLQK S H 
Sbjct: 1   MTGLQRTKSCSAAIQTTTKIIPSSISAHHLRSS--------SDADNFSGKFLLQKNSSHR 60

Query: 66  ------AASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSLRRKVTGTLFG 125
                 AAST GKFSSLLHSLLKLLS     IPTCKWLSIPTHLSVSPSL R+VTGTLFG
Sbjct: 61  CHRQHLAASTTGKFSSLLHSLLKLLS-----IPTCKWLSIPTHLSVSPSLGRRVTGTLFG 120

Query: 126 HRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPA--DDGRP-RR 185
            RRGHVTFA+QLDPRS+PVLLLE ATSTSSL+KEMSSGLVRIALES+ P   D  RP R+
Sbjct: 121 RRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRK 180

Query: 186 LFQEPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGSGTEGE 245
           L +EPRWTMYCNGRKCG A SR CGEF+W+VLNTVQSVSVGAGVIP+VDDGRKGSG+EGE
Sbjct: 181 LLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGE 240

Query: 246 LLYMRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
           LLYMRA+FERV+GSRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 LLYMRAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of Sgr017295 vs. ExPASy TrEMBL
Match: A0A6J1HPB2 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466084 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.7e-109
Identity = 218/280 (77.86%), Postives = 233/280 (83.21%), Query Frame = 0

Query: 6   LTTLQRTRSCSAAIKSTRKIIPASSSGHHLRSSSSNLPPEYSDAGNLSGKFLLQKKSDHP 65
           +T LQRT+SCSAAIK+T KIIP+S SGHHLRSS        SDA N S KFLLQK S H 
Sbjct: 1   MTGLQRTKSCSAAIKTTTKIIPSSISGHHLRSS--------SDADNFSAKFLLQKNSSHR 60

Query: 66  ------AASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSLRRKVTGTLFG 125
                 AAST GKFSSLLHSLLKL+S     IPTCKWLSIPTHLSVSPSL R+VTGTLFG
Sbjct: 61  CHRQHLAASTTGKFSSLLHSLLKLIS-----IPTCKWLSIPTHLSVSPSLGRRVTGTLFG 120

Query: 126 HRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESE--TPADDGRP-RR 185
            RRGHVTFAVQLDPRSEPVLLLE ATSTSSL+KEMSSGLVRIALES+  T  D  R  R+
Sbjct: 121 RRRGHVTFAVQLDPRSEPVLLLEFATSTSSLIKEMSSGLVRIALESDALTTYDGNRSIRK 180

Query: 186 LFQEPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGSGTEGE 245
           L +EPRWTMYCNGRKCG A SR CGEF+W+VLNTVQSVSVGAGVIP+VDDG  GS +EGE
Sbjct: 181 LLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGHMGSRSEGE 240

Query: 246 LLYMRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
           LLYMRA+FERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 LLYMRAKFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of Sgr017295 vs. ExPASy TrEMBL
Match: A0A6J1DFH1 (protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111020015 PE=4 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 2.0e-107
Identity = 213/280 (76.07%), Postives = 231/280 (82.50%), Query Frame = 0

Query: 6   LTTLQRTRSCSAAIKSTRKIIPASS-SGH-----HLRSSSSNLPPEYSDAGNLSGKFLLQ 65
           ++TLQRTRSCSAAIK+T KI PA+S SGH     H+RSSSS        +   SG  LLQ
Sbjct: 1   MSTLQRTRSCSAAIKTTTKITPAASLSGHHHRHRHVRSSSS--------SDGFSGNLLLQ 60

Query: 66  KKSDHPAASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSLRRKVTGTLFG 125
           KKS        GKFSSL HSLL+LLS     IPTCKWLSIP HLS +PSLRRKVTGTLFG
Sbjct: 61  KKSGDHHHHPAGKFSSLFHSLLRLLS-----IPTCKWLSIPNHLSATPSLRRKVTGTLFG 120

Query: 126 HRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPADDGRPRRLFQ 185
           HRRGHVTFAVQL+PRSEPV LLELA ST+SLVKEMSSGLVRIALESE PA++   RRLF+
Sbjct: 121 HRRGHVTFAVQLEPRSEPVFLLELAVSTASLVKEMSSGLVRIALESEAPANEDGRRRLFE 180

Query: 186 EPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGS---GTEGE 245
           EPRWTMYCNGRKCG+ATSR CG+F+WHVLNTVQSVSVGAGVIPVVDDGRKG    G++GE
Sbjct: 181 EPRWTMYCNGRKCGHATSRTCGDFEWHVLNTVQSVSVGAGVIPVVDDGRKGGGGCGSDGE 240

Query: 246 LLYMRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
           LLYMRARFERVVGS DSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 LLYMRARFERVVGSHDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of Sgr017295 vs. ExPASy TrEMBL
Match: A0A5A7SPQ9 (Protein MIZU-KUSSEI 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1702G00210 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 2.4e-100
Identity = 206/277 (74.37%), Postives = 226/277 (81.59%), Query Frame = 0

Query: 6   LTTLQRTRSCSAAIKSTRKIIPASSSGHH---LRS-SSSNLPPEYSDAGNLSGKFLLQKK 65
           +T LQRTRS SAAIK+T K+IP+S SGH     RS SSSNLPPEYS+  N SG       
Sbjct: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSG-----HH 60

Query: 66  SDHPAASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSL-RRKVTGTLFGH 125
                  T  KFS LLHSLLKLLS     +PTCKWLSIPTHLSV PSL RR VTGTLFG 
Sbjct: 61  GHRHQTVTGSKFSILLHSLLKLLS-----VPTCKWLSIPTHLSVRPSLGRRVVTGTLFGR 120

Query: 126 RRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPA-DDGRPRRLFQ 185
           RRGHVTFAVQ+ P+SEPVLLLELATSTSSLVKEMSSGLVRIALESE PA +DGRP++LF+
Sbjct: 121 RRGHVTFAVQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFE 180

Query: 186 EPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGSGTEGELLY 245
           EPRWTMYCNGRKCG++  R CGEFD +VLN VQSVSVGAGVIPVV++G K S +EGELLY
Sbjct: 181 EPRWTMYCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLY 240

Query: 246 MRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
           MRA+FERVVGSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 MRAKFERVVGSRDSEAFYMINPDSNGGPELSIFLLRI 267

BLAST of Sgr017295 vs. ExPASy TrEMBL
Match: A0A1S3CNY4 (protein MIZU-KUSSEI 1-like OS=Cucumis melo OX=3656 GN=LOC103502949 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 2.4e-100
Identity = 206/277 (74.37%), Postives = 226/277 (81.59%), Query Frame = 0

Query: 6   LTTLQRTRSCSAAIKSTRKIIPASSSGHH---LRS-SSSNLPPEYSDAGNLSGKFLLQKK 65
           +T LQRTRS SAAIK+T K+IP+S SGH     RS SSSNLPPEYS+  N SG       
Sbjct: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSG-----HH 60

Query: 66  SDHPAASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSVSPSL-RRKVTGTLFGH 125
                  T  KFS LLHSLLKLLS     +PTCKWLSIPTHLSV PSL RR VTGTLFG 
Sbjct: 61  GHRHQTVTGSKFSILLHSLLKLLS-----VPTCKWLSIPTHLSVRPSLGRRVVTGTLFGR 120

Query: 126 RRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPA-DDGRPRRLFQ 185
           RRGHVTFAVQ+ P+SEPVLLLELATSTSSLVKEMSSGLVRIALESE PA +DGRP++LF+
Sbjct: 121 RRGHVTFAVQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFE 180

Query: 186 EPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPVVDDGRKGSGTEGELLY 245
           EPRWTMYCNGRKCG++  R CGEFD +VLN VQSVSVGAGVIPVV++G K S +EGELLY
Sbjct: 181 EPRWTMYCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLY 240

Query: 246 MRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
           MRA+FERVVGSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 MRAKFERVVGSRDSEAFYMINPDSNGGPELSIFLLRI 267

BLAST of Sgr017295 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 255.0 bits (650), Expect = 7.0e-68
Identity = 169/310 (54.52%), Postives = 197/310 (63.55%), Query Frame = 0

Query: 4   QELTTLQRTRSCSAAIKSTRKIIPASSSGH--HLRS-SSSNLPPEYSDAGNLSGKFLL-- 63
           QEL TLQR+ S      ++RKI P +S     H+RS SSS L P   +       FL+  
Sbjct: 5   QEL-TLQRSFS-----YNSRKINPVTSPARSSHVRSPSSSALIPSIPE----HELFLVPC 64

Query: 64  ----------QKKSDHPAASTRGKFSSLLHSLLKLLSFPTLNIPTCKWLSIPTHLSV--- 123
                        + H       KFS LL S + +     +NIP CK LS+P+  S    
Sbjct: 65  RRCSYVPLSSSSSASHNIGKFHLKFSLLLRSFINI-----INIPACKMLSLPSPPSSSSS 124

Query: 124 ------------SPSLRRKVTGTLFGHRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKE 183
                       S SL R+VTGTL+GH+RGHVTF+VQ + RS+PVLLL+LA ST++LVKE
Sbjct: 125 VSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKE 184

Query: 184 MSSGLVRIALESETPADDGRPRRLFQEPRWTMYCNGRKCGYATSR--ACGEFDWHVLNTV 243
           MSSGLVRIALE E     G   +LFQEP+WTMYCNGRKCGYA SR  AC + DW VLNTV
Sbjct: 185 MSSGLVRIALECEKRHRSG--TKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTV 244

Query: 244 QSVSVGAGVIP---VVDD-GRKGSGTE-GELLYMRARFERVVGSRDSEAFYMMNPDGNGG 277
             V+VGAGVIP    +DD    GSGTE GELLYMR +FERVVGSRDSEAFYMMNPD NGG
Sbjct: 245 SRVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGG 297

BLAST of Sgr017295 vs. TAIR 10
Match: AT5G23100.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 203.4 bits (516), Expect = 2.4e-52
Identity = 115/249 (46.18%), Postives = 148/249 (59.44%), Query Frame = 0

Query: 53  SGKFLLQKKSDHPAASTRGKFSSLLHSLLKLLSFPTLNI-PTCKWLSIPTHLSVSPSLRR 112
           S K L    +  P++S+    S+++    KL SF    +      LS     + +  L  
Sbjct: 29  SHKPLPHHNNTKPSSSSSSSSSNVITHKKKLQSFAVSRLRSVIATLSRARPGNQNSGLGS 88

Query: 113 KVTGTLFGHRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALE------- 172
           +V GTLFG RRGHV F++Q DP S P  L+ELAT  S LVKEM+SGLVRIALE       
Sbjct: 89  RVVGTLFGSRRGHVHFSIQKDPNSPPAFLIELATPISGLVKEMASGLVRIALECDKGKEE 148

Query: 173 -----------------SETPADDGRPRRLFQEPRWTMYCNGRKCGYATSRACGEFDWHV 232
                            ++T       RRL +EP W  YCNG+KCG+AT R CGE +  V
Sbjct: 149 EEGEEKNGTLRHGGGDKTKTTTTAAVSRRLVEEPMWRTYCNGKKCGFATRRECGEKEKKV 208

Query: 233 LNTVQSVSVGAGVIPVVDDGRKGSGTEGELLYMRARFERVVGSRDSEAFYMMNPDGNGGP 277
           L  ++ VS+GAGV+P  ++   G G  G+++YMRA+FER+VGSRDSEAFYMMNPD NG P
Sbjct: 209 LKALEMVSMGAGVLPETEEIGGGGGGGGDIMYMRAKFERIVGSRDSEAFYMMNPDSNGAP 268

BLAST of Sgr017295 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 197.6 bits (501), Expect = 1.3e-50
Identity = 100/172 (58.14%), Postives = 127/172 (73.84%), Query Frame = 0

Query: 112 KVTGTLFGHRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPADD 171
           +V GTLFG+RRGHV FAVQ DP   P +L++L T TS LV+EM+SGLVRIALE+     D
Sbjct: 97  RVVGTLFGNRRGHVYFAVQDDPTRLPAVLIQLPTPTSVLVREMASGLVRIALETAAYKTD 156

Query: 172 GRPRRLFQEPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIP-----VVDD 231
            + ++L +E  W  YCNG+KCGYA  + CGE +W VL  V  +++GAGV+P     V ++
Sbjct: 157 SK-KKLLEESTWRTYCNGKKCGYAARKECGEAEWKVLKAVGPITMGAGVLPATTTTVDEE 216

Query: 232 GRKGSGTE-GELLYMRARFERVVGSRDSEAFYMMNPD-GNGGPELSIFLLRI 277
           G    G+E GEL+YMRARFERVVGSRDSEAFYMMNPD  +GGPELS++ LR+
Sbjct: 217 GNGAVGSEKGELMYMRARFERVVGSRDSEAFYMMNPDVSSGGPELSVYFLRV 267

BLAST of Sgr017295 vs. TAIR 10
Match: AT5G06990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 175.6 bits (444), Expect = 5.4e-44
Identity = 90/172 (52.33%), Postives = 114/172 (66.28%), Query Frame = 0

Query: 112 KVTGTLFGHRRGHVTFAVQLDPRSEPVLLLELATSTSSLVKEMSSGLVRIALESETPADD 171
           +VTGTLFG+R+  V  AVQ +PRS P+LLLELA  T  L++++  GLVRIALE E    +
Sbjct: 92  RVTGTLFGYRKTRVNLAVQENPRSLPILLLELAIPTGKLLQDLGVGLVRIALECEKKPSE 151

Query: 172 GRPRRLFQEPRWTMYCNGRKCGYATSRACGEFDWHVLNTVQSVSVGAGVIPV-------V 231
               ++  EP W +YCNG+K GY   R   E D  V+  + +VS+GAGV+PV        
Sbjct: 152 --KTKIIDEPIWALYCNGKKSGYGVKRQPTEEDLVVMQMLHAVSMGAGVLPVSSGAITEQ 211

Query: 232 DDGRKGSGTEGELLYMRARFERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 277
             G  G   EG+L YMRA FERV+GSRDSE +YMMNPDGN GPELSIF +R+
Sbjct: 212 SGGGGGGQQEGDLTYMRAHFERVIGSRDSETYYMMNPDGNSGPELSIFFVRV 261

BLAST of Sgr017295 vs. TAIR 10
Match: AT4G39610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 169.5 bits (428), Expect = 3.8e-42
Identity = 102/254 (40.16%), Postives = 150/254 (59.06%), Query Frame = 0

Query: 37  SSSSNLPPEYSDAGNLSGKF-LLQKKSDHPAASTRGKFSSLLHSLLKLL--SFPTLNIPT 96
           SSSS++PP        S      Q     P +S + K  + +  +L+ +  SFP    P+
Sbjct: 14  SSSSSIPPSSPTPSPTSTTTPPRQHPLLQPPSSKKKKNRTNVFRVLRTVFRSFPIFTTPS 73

Query: 97  --CKWLSIPTHLSVSPSLRR--KVTGTLFGHRRGHVTFAVQLDPRSEPVLLLELATSTSS 156
             CK   I   L +        ++TGTLFG+R+G V+ ++Q +P+  P L++ELA  T++
Sbjct: 74  VACKIPVIHPGLGLPDPHHNTSRITGTLFGYRKGRVSLSIQENPKCLPSLVVELAMQTTT 133

Query: 157 LVKEMSSGLVRIALESETP--ADDGRPRR-----LFQEPRWTMYCNGRKCGYATSRACGE 216
           L KE+S+G+VRIALE+E    AD+   +      + +EP WTMYC G K GY   R   E
Sbjct: 134 LQKELSTGMVRIALETEKQPRADNNNSKTEKKTDILEEPLWTMYCKGEKTGYGVKREATE 193

Query: 217 FDWHVLNTVQSVSVGAGVIPVVDDGRKGSGTEGELLYMRARFERVVGSRDSEAFYMMNPD 276
            D +V+  ++ VS+GAGV+P      +  G +GE+ YMRA FERV+GS+DSE FYM++P+
Sbjct: 194 EDLNVMELLRPVSMGAGVLP---GNSESEGPDGEMAYMRAYFERVIGSKDSETFYMLSPE 253

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022924911.19.6e-11278.21protein MIZU-KUSSEI 1 [Cucurbita moschata][more]
KAG7027634.11.6e-11178.21Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023518638.16.2e-11178.57protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo][more]
XP_038877466.11.5e-10977.03protein MIZU-KUSSEI 1-like [Benincasa hispida][more]
XP_022966431.17.6e-10977.86protein MIZU-KUSSEI 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O222279.8e-6754.52Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EAS04.7e-11278.21protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111432316 PE=4 SV=1[more]
A0A6J1HPB23.7e-10977.86protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466084 PE=4 SV=1[more]
A0A6J1DFH12.0e-10776.07protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111020015 PE=4 SV=1[more]
A0A5A7SPQ92.4e-10074.37Protein MIZU-KUSSEI 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3CNY42.4e-10074.37protein MIZU-KUSSEI 1-like OS=Cucumis melo OX=3656 GN=LOC103502949 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G41660.17.0e-6854.52Protein of unknown function, DUF617 [more]
AT5G23100.12.4e-5246.18Protein of unknown function, DUF617 [more]
AT3G25640.11.3e-5058.14Protein of unknown function, DUF617 [more]
AT5G06990.15.4e-4452.33Protein of unknown function, DUF617 [more]
AT4G39610.13.8e-4240.16Protein of unknown function, DUF617 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006460Protein MIZU-KUSSEI 1-like, plantPFAMPF04759DUF617coord: 113..275
e-value: 3.6E-69
score: 232.0
IPR006460Protein MIZU-KUSSEI 1-like, plantTIGRFAMTIGR01570TIGR01570coord: 113..276
e-value: 1.9E-70
score: 233.9
IPR006460Protein MIZU-KUSSEI 1-like, plantPANTHERPTHR31696PROTEIN MIZU-KUSSEI 1coord: 2..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..46
NoneNo IPR availablePANTHERPTHR31696:SF71PROTEIN MIZU-KUSSEI 1coord: 2..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr017295.1Sgr017295.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010274 hydrotropism
cellular_component GO:0098827 endoplasmic reticulum subcompartment
cellular_component GO:0016020 membrane