Sgr017260 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr017260
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Calcium-binding protein CML36
Location	tig00153037: 108515 .. 109171 (+)
RNA-Seq Expression	Sgr017260
Synteny	Sgr017260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACTCCCCAAGCGATTCACCCCCAAGCGCCTCTTCCGATCCAAGAAGAGCCGCGCCGACGTTTCTCGCTCGGACCCGCCGTCGTTCGGCTCCGGTACGTCGTCTTCTTCTTCTTCTTCTGAGAACTTCTTCAAGCCCGAGACTCTCTCTTGCGTTGGAACTCCGACCAGCGTCTTGCCCGATTCGTCGGCGGACTGGTCCGACCTGTCGGCCGACTCGAACTCCGAGCTCGTCCAGGCGTTTAAGCTGATCGACAGGGATAACGATGGGGTTGTGTCGAGAAAAGAGCTGGAGGCTATTTTGTGCCGGATCGGGGCGGAGCCGCCGACGGAAGAGGAGGTGGCCATGATGCTTAACGAGGTGGACGACGACGGCGATGGGTTTATCTCCCTGGAGGCGCTGATGGCCAGAATCGTCATGTCGGCGGCTTCCGGGCCGGCCGGAGACTCGGAGCTCCTGGAGACGTTTGAGTTTTTCGACTCCGACCACGACGGCAGGATCACGGCGGAGGAGCTGCTCAGTGTGTTCAGTTCCATGGGAGACGAGGGGTGCACGTTAGACGACTGCCGGCGCATGATAGCGGATGTGGATGAGAACGGGGACGGTTTCGTGTGCTTCGCGGAATTCGTGCGTATGATGGGGCTGCAGAGATGA

mRNA sequence

ATGAAACTCCCCAAGCGATTCACCCCCAAGCGCCTCTTCCGATCCAAGAAGAGCCGCGCCGACGTTTCTCGCTCGGACCCGCCGTCGTTCGGCTCCGGTACGTCGTCTTCTTCTTCTTCTTCTGAGAACTTCTTCAAGCCCGAGACTCTCTCTTGCGTTGGAACTCCGACCAGCGTCTTGCCCGATTCGTCGGCGGACTGGTCCGACCTGTCGGCCGACTCGAACTCCGAGCTCGTCCAGGCGTTTAAGCTGATCGACAGGGATAACGATGGGGTTGTGTCGAGAAAAGAGCTGGAGGCTATTTTGTGCCGGATCGGGGCGGAGCCGCCGACGGAAGAGGAGGTGGCCATGATGCTTAACGAGGTGGACGACGACGGCGATGGGTTTATCTCCCTGGAGGCGCTGATGGCCAGAATCGTCATGTCGGCGGCTTCCGGGCCGGCCGGAGACTCGGAGCTCCTGGAGACGTTTGAGTTTTTCGACTCCGACCACGACGGCAGGATCACGGCGGAGGAGCTGCTCAGTGTGTTCAGTTCCATGGGAGACGAGGGGTGCACGTTAGACGACTGCCGGCGCATGATAGCGGATGTGGATGAGAACGGGGACGGTTTCGTGTGCTTCGCGGAATTCGTGCGTATGATGGGGCTGCAGAGATGA

Coding sequence (CDS)

ATGAAACTCCCCAAGCGATTCACCCCCAAGCGCCTCTTCCGATCCAAGAAGAGCCGCGCCGACGTTTCTCGCTCGGACCCGCCGTCGTTCGGCTCCGGTACGTCGTCTTCTTCTTCTTCTTCTGAGAACTTCTTCAAGCCCGAGACTCTCTCTTGCGTTGGAACTCCGACCAGCGTCTTGCCCGATTCGTCGGCGGACTGGTCCGACCTGTCGGCCGACTCGAACTCCGAGCTCGTCCAGGCGTTTAAGCTGATCGACAGGGATAACGATGGGGTTGTGTCGAGAAAAGAGCTGGAGGCTATTTTGTGCCGGATCGGGGCGGAGCCGCCGACGGAAGAGGAGGTGGCCATGATGCTTAACGAGGTGGACGACGACGGCGATGGGTTTATCTCCCTGGAGGCGCTGATGGCCAGAATCGTCATGTCGGCGGCTTCCGGGCCGGCCGGAGACTCGGAGCTCCTGGAGACGTTTGAGTTTTTCGACTCCGACCACGACGGCAGGATCACGGCGGAGGAGCTGCTCAGTGTGTTCAGTTCCATGGGAGACGAGGGGTGCACGTTAGACGACTGCCGGCGCATGATAGCGGATGTGGATGAGAACGGGGACGGTTTCGTGTGCTTCGCGGAATTCGTGCGTATGATGGGGCTGCAGAGATGA

Protein sequence

MKLPKRFTPKRLFRSKKSRADVSRSDPPSFGSGTSSSSSSSENFFKPETLSCVGTPTSVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVAMMLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEELLSVFSSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR

Homology

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BLAST of Sgr017260 vs. NCBI nr
Match: XP_022153870.1 (probable calcium-binding protein CML36 [Momordica charantia])

HSP 1 Score: 277.7 bits (709), Expect = 8.5e-71
Identity = 157/221 (71.04%), Postives = 175/221 (79.19%), Query Frame = 0


Query: 1   MKLPKRFTPKRLFRSKKSRADVS-RSDPPSFGSGTSSSSSSSENFFKPETLSCV---GTP 60
           MK+PKRF+PKRLFRSKK+RADVS RSDPPSFGS TSSSSSSSENFFKP+T +     GTP
Sbjct: 1   MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP 60

Query: 61  TSVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVA 120
           TSVLPDS+ADWS                   +N+GVVSRKELEAIL RIGA+PP+EEEVA
Sbjct: 61  TSVLPDSAADWS-------------------ENNGVVSRKELEAILHRIGADPPSEEEVA 120

Query: 121 MMLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEELLSV 180
           MML E   DGDGFI LEALM R+   +  GPA ++EL E FEFFD+DHDGRITAEEL  V
Sbjct: 121 MMLGEF--DGDGFIRLEALMDRV--GSGFGPACEAELREAFEFFDADHDGRITAEELHGV 180

Query: 181 FSSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQ 218
           FSS+GDE CTL+DCRRMIADVDENGDGFVCF+EFVRMM LQ
Sbjct: 181 FSSIGDERCTLEDCRRMIADVDENGDGFVCFSEFVRMMELQ 198

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BLAST of Sgr017260 vs. NCBI nr
Match: KAF3947310.1 (hypothetical protein CMV_026538 [Castanea mollissima])

HSP 1 Score: 269.2 bits (687), Expect = 3.0e-68
Identity = 145/228 (63.60%), Postives = 181/228 (79.39%), Query Frame = 0


Query: 1   MKLPK--RFTPKRLFRSKKSRADVSRSDPPSFGSGTSSSSSSSE-----NFFKPET---L 60
           MKLPK  + +PKRLF SKK+R DVSR DP SFGS TSS+SSSS      +  KP +   +
Sbjct: 1   MKLPKSLKLSPKRLFGSKKNRPDVSRHDPLSFGSSTSSTSSSSSSDSCTSLHKPGSGTKI 60

Query: 61  SCVGTPTSVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPP 120
           + +GTP SVLP  S DW ++SA+ + ELVQAFKL+DRDNDG+VSRKELEA+L R+GAEP 
Sbjct: 61  ANIGTPKSVLPQESGDWGEISAEKHMELVQAFKLMDRDNDGMVSRKELEALLSRLGAEPL 120

Query: 121 TEEEVAMMLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITA 180
           ++EEV MML++VD +G G IS++AL+ R+   +A GPAGD+EL ETF+FFD+DHDG+ITA
Sbjct: 121 SQEEVTMMLSDVDGEGHGCISVDALLTRV--GSACGPAGDTELRETFDFFDTDHDGKITA 180

Query: 181 EELLSVFSSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR 219
           EELLSVF ++GDE CTL+DCRRMI  VD+NGDGFVCF +F RMM LQR
Sbjct: 181 EELLSVFQTIGDERCTLEDCRRMIDGVDKNGDGFVCFEDFTRMMELQR 226

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BLAST of Sgr017260 vs. NCBI nr
Match: XP_024181636.1 (probable calcium-binding protein CML36 [Rosa chinensis] >PRQ49697.1 putative EF-hand domain pair protein [Rosa chinensis])

HSP 1 Score: 265.8 bits (678), Expect = 3.3e-67
Identity = 149/222 (67.12%), Postives = 174/222 (78.38%), Query Frame = 0


Query: 1   MKLPK--RFTPKRLFRSKKSRADVSRSDPPSFGSGTSSSSSSS--ENFFKPETLSCVGTP 60
           MKL K    +PKRLFRS K R+ VSRSDPPSFGSGT+SSSSS    +  KP     V TP
Sbjct: 1   MKLMKINSLSPKRLFRSMKDRSAVSRSDPPSFGSGTASSSSSDPLPSDHKPGPGGMV-TP 60

Query: 61  TSVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVA 120
           TSVLP+ S DWSD SAD++ ++ QAFKLIDRDNDGVVSRKELEA+L R+GAEPP+ EEVA
Sbjct: 61  TSVLPEISGDWSDFSADTHLDMAQAFKLIDRDNDGVVSRKELEALLKRLGAEPPSHEEVA 120

Query: 121 MMLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEELLSV 180
           MML+EVD DGDG I LEAL+ R     A  PA DSEL E FE FD+DHDG+I+AEEL SV
Sbjct: 121 MMLSEVDRDGDGCIPLEALLHR--FGPACEPAADSELREAFEVFDADHDGKISAEELFSV 180

Query: 181 FSSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR 219
           F+++GD+ CTL+DCRRMIA+VD+NGDGFVCF +F  MM LQR
Sbjct: 181 FTAIGDDRCTLEDCRRMIAEVDKNGDGFVCFEDFALMMELQR 219

[Close]

BLAST of Sgr017260 vs. NCBI nr
Match: XP_030949696.1 (probable calcium-binding protein CML36 [Quercus lobata])

HSP 1 Score: 265.0 bits (676), Expect = 5.7e-67
Identity = 144/225 (64.00%), Postives = 179/225 (79.56%), Query Frame = 0


Query: 1   MKLPK--RFTPKRLFRSKKSRADVSRSDPPSFGSGTSSSSS--SSENFFKPET---LSCV 60
           MKLPK  + +PKRLF SKK+R D+SR DP SFGS TSSSSS  SS +  KP +    + +
Sbjct: 1   MKLPKSLKLSPKRLFGSKKNRPDLSRHDPLSFGSSTSSSSSSDSSASLKKPGSGTEFANI 60

Query: 61  GTPTSVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEE 120
           GTP SVLP  S DW ++SA+ + ELVQAFKL+DRDNDG+VSRKELEA+L R+GAEP ++E
Sbjct: 61  GTPKSVLPQVSGDWGEISAEKHMELVQAFKLMDRDNDGMVSRKELEALLSRLGAEPLSQE 120

Query: 121 EVAMMLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEEL 180
           EV MML++VD +G G IS++AL+ R+   +  GPAGD+EL ETF+FFD+DHDG+ITAEEL
Sbjct: 121 EVTMMLSDVDGEGHGCISVDALLTRV--GSECGPAGDTELRETFDFFDTDHDGKITAEEL 180

Query: 181 LSVFSSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR 219
           LSVF ++GDE CTL+DCRRMI  VD+NGDGFVCF +F RMM LQR
Sbjct: 181 LSVFQTIGDERCTLEDCRRMIDGVDKNGDGFVCFEDFSRMMELQR 223

[Close]

BLAST of Sgr017260 vs. NCBI nr
Match: KAF3963812.1 (hypothetical protein CMV_011844 [Castanea mollissima])

HSP 1 Score: 264.6 bits (675), Expect = 7.4e-67
Identity = 140/221 (63.35%), Postives = 176/221 (79.64%), Query Frame = 0


Query: 6   RFTPKRLFRSKKSRADVSRSDPPSFGSGTSSSSSSSE-----NFFKPET---LSCVGTPT 65
           + +PKRLF SKK+R DVSR DP SFGS TSS+SSSS      +  KP +   ++ +GTP 
Sbjct: 10  KVSPKRLFGSKKNRPDVSRHDPLSFGSSTSSTSSSSSSDSCTSLHKPGSGTKIANIGTPK 69

Query: 66  SVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVAM 125
           SVLP  S DW ++SA+ + ELVQAFKL+DRDNDG+VSRKELEA+L R+GAEP ++EEV M
Sbjct: 70  SVLPQESGDWGEISAEKHMELVQAFKLMDRDNDGMVSRKELEALLSRLGAEPLSQEEVTM 129

Query: 126 MLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEELLSVF 185
           ML++VD +G G IS++AL+ R+   +A GPAGD+EL ETF+FFD+DHDG+ITAEELLSVF
Sbjct: 130 MLSDVDGEGHGCISVDALLTRV--GSACGPAGDTELRETFDFFDTDHDGKITAEELLSVF 189

Query: 186 SSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR 219
            ++GDE CTL+DCRRMI  VD+NGDGFVCF +F RMM LQR
Sbjct: 190 QTIGDERCTLEDCRRMIDGVDKNGDGFVCFEDFTRMMELQR 228

[Close]

BLAST of Sgr017260 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 7.0e-52
Identity = 126/220 (57.27%), Postives = 154/220 (70.00%), Query Frame = 0


Query: 1   MKLPKRFTPKRLFRSKKSRADVSRSDPP-SFGSGTSSSSSSSENFFKPETLSCVGTPTSV 60
           MKL K   PKR F   K R+ VS+S    SFGS +SSS    +N         V TPTS+
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSV-TPTSI 60

Query: 61  LPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVAMML 120
           LP+  + +S +      E++QAFKLIDRDNDG VSR +LE++L R+G +P TEEE+ +ML
Sbjct: 61  LPEVPSPYSYV------EILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVML 120

Query: 121 NEVDDDGDGFISLEALMARIVMSAASGPAGDS-ELLETFEFFDSDHDGRITAEELLSVFS 180
            EVD DGDG I LE L +R+V   +  PA DS EL ETFEFFD+D DG I+A+ELL VFS
Sbjct: 121 KEVDCDGDGTIRLEELASRVV---SLDPARDSTELKETFEFFDADRDGLISADELLRVFS 180

Query: 181 SMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR 219
           ++GDE CTLDDC+RMIADVDE+GDGFVCF EF RMM LQR
Sbjct: 181 TIGDERCTLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Sgr017260 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 6.2e-48
Identity = 112/209 (53.59%), Postives = 143/209 (68.42%), Query Frame = 0


Query: 6   RFTPKRLFRSKKSRADVSRSDPPSFGSGTSSSSSSSENFFKPETLSCVGTPTSVLPDSSA 65
           R +PKRLFR+ KS+A VSRS+P SF S  ++SSSSS+  +         TP SVLP +S 
Sbjct: 9   RLSPKRLFRT-KSKASVSRSEPSSFSS--NASSSSSDGSYGNLKQGPTATPISVLPQNSG 68

Query: 66  DWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVAMMLNEVDDD 125
           D+        +ELVQAFKLIDRD+DGVVSR +L A++ R+  EPP++EEV++ML EVD  
Sbjct: 69  DF-------YTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGG 128

Query: 126 GDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEELLSVFSSMGDEGC 185
             G ISLE L +R+  ++  G     EL E FE FD D +G+I+AEEL  VF  +GDE C
Sbjct: 129 DGGCISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERC 188

Query: 186 TLDDCRRMIADVDENGDGFVCFAEFVRMM 215
           TL++C RMIA VD NGDGFVCF +F RMM
Sbjct: 189 TLEECMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Sgr017260 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.9e-20
Identity = 61/163 (37.42%), Postives = 89/163 (54.60%), Query Frame = 0


Query: 54  GTPTSVLP--DSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPT 113
           G+ +  LP  D ++     +     E+ + F+  D + DG +SR EL A+   +G    T
Sbjct: 15  GSKSPPLPQADPASGGGSPAPTPEEEMERVFRKFDANGDGRISRSELGALFESLG-HAAT 74

Query: 114 EEEVAMMLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAE 173
           ++E+A M+ E D DGDGFISL+   A    ++    A + +L   F  FD+D +G I+A 
Sbjct: 75  DDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAA 134

Query: 174 ELLSVFSSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMM 215
           EL  V   +G E  T+  CRRMI  VD+NGDG + F EF  MM
Sbjct: 135 ELARVLHGLG-EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]

BLAST of Sgr017260 vs. ExPASy Swiss-Prot
Match: Q5ZCK5 (Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 GN=CML16 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.9e-20
Identity = 65/148 (43.92%), Postives = 86/148 (58.11%), Query Frame = 0


Query: 72  ADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTE----EEVAMMLNEVDDDGD 131
           A +++E+ + F   D D DG +S  EL A+  R  A PP+E     EVA M+NE+D D D
Sbjct: 22  APADAEIERVFTRFDADGDGRISPSELAAV-TRAIAPPPSESAGGREVAAMMNELDTDRD 81

Query: 132 GFISLEALMARIVMSAASGPA-GDSELLETFEFFDSDHDGRITAEELLSVFSSMGDEGCT 191
           GF+ L    A        G A  ++EL   F+ +D D DGRITA EL  V   +G EGC+
Sbjct: 82  GFVDLGEFAA--FHGRGRGDAEHEAELRAAFDVYDVDGDGRITAAELGKVLGRIG-EGCS 141

Query: 192 LDDCRRMIADVDENGDGFVCFAEFVRMM 215
            ++C RMIA VD +GDG V F EF +MM
Sbjct: 142 AEECERMIASVDVDGDGCVGFEEFKKMM 165

[Close]

BLAST of Sgr017260 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 7.1e-20
Identity = 59/144 (40.97%), Postives = 89/144 (61.81%), Query Frame = 0


Query: 74  SNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPP--TEEEVAMMLNEVDDDGDGFIS 133
           S  ++ + F+  D++NDG +S  EL+ +   IGA  P  ++EE   M+ E D DG+GFI 
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDV---IGALSPNASQEETKAMMKEFDLDGNGFID 71

Query: 134 LEALMARIVMS-AASGPAGDSELLETFEFFDSDHDGRITAEELLSVFSSMGDEGCTLDDC 193
           L+  +A   +S  +S  +   +L E F+ +D D +GRI+A EL SV  ++G E C++ DC
Sbjct: 72  LDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDC 131

Query: 194 RRMIADVDENGDGFVCFAEFVRMM 215
           +RMI  VD +GDG V F EF +MM
Sbjct: 132 QRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Sgr017260 vs. ExPASy TrEMBL
Match: A0A6J1DI26 (probable calcium-binding protein CML36 OS=Momordica charantia OX=3673 GN=LOC111021283 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 4.1e-71
Identity = 157/221 (71.04%), Postives = 175/221 (79.19%), Query Frame = 0


Query: 1   MKLPKRFTPKRLFRSKKSRADVS-RSDPPSFGSGTSSSSSSSENFFKPETLSCV---GTP 60
           MK+PKRF+PKRLFRSKK+RADVS RSDPPSFGS TSSSSSSSENFFKP+T +     GTP
Sbjct: 1   MKIPKRFSPKRLFRSKKNRADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGTP 60

Query: 61  TSVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVA 120
           TSVLPDS+ADWS                   +N+GVVSRKELEAIL RIGA+PP+EEEVA
Sbjct: 61  TSVLPDSAADWS-------------------ENNGVVSRKELEAILHRIGADPPSEEEVA 120

Query: 121 MMLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEELLSV 180
           MML E   DGDGFI LEALM R+   +  GPA ++EL E FEFFD+DHDGRITAEEL  V
Sbjct: 121 MMLGEF--DGDGFIRLEALMDRV--GSGFGPACEAELREAFEFFDADHDGRITAEELHGV 180

Query: 181 FSSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQ 218
           FSS+GDE CTL+DCRRMIADVDENGDGFVCF+EFVRMM LQ
Sbjct: 181 FSSIGDERCTLEDCRRMIADVDENGDGFVCFSEFVRMMELQ 198

[Close]

BLAST of Sgr017260 vs. ExPASy TrEMBL
Match: A0A2P6RTD5 (Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0124821 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.6e-67
Identity = 149/222 (67.12%), Postives = 174/222 (78.38%), Query Frame = 0


Query: 1   MKLPK--RFTPKRLFRSKKSRADVSRSDPPSFGSGTSSSSSSS--ENFFKPETLSCVGTP 60
           MKL K    +PKRLFRS K R+ VSRSDPPSFGSGT+SSSSS    +  KP     V TP
Sbjct: 1   MKLMKINSLSPKRLFRSMKDRSAVSRSDPPSFGSGTASSSSSDPLPSDHKPGPGGMV-TP 60

Query: 61  TSVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVA 120
           TSVLP+ S DWSD SAD++ ++ QAFKLIDRDNDGVVSRKELEA+L R+GAEPP+ EEVA
Sbjct: 61  TSVLPEISGDWSDFSADTHLDMAQAFKLIDRDNDGVVSRKELEALLKRLGAEPPSHEEVA 120

Query: 121 MMLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEELLSV 180
           MML+EVD DGDG I LEAL+ R     A  PA DSEL E FE FD+DHDG+I+AEEL SV
Sbjct: 121 MMLSEVDRDGDGCIPLEALLHR--FGPACEPAADSELREAFEVFDADHDGKISAEELFSV 180

Query: 181 FSSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR 219
           F+++GD+ CTL+DCRRMIA+VD+NGDGFVCF +F  MM LQR
Sbjct: 181 FTAIGDDRCTLEDCRRMIAEVDKNGDGFVCFEDFALMMELQR 219

[Close]

BLAST of Sgr017260 vs. ExPASy TrEMBL
Match: A0A7N2RFF9 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.8e-67
Identity = 144/225 (64.00%), Postives = 179/225 (79.56%), Query Frame = 0


Query: 1   MKLPK--RFTPKRLFRSKKSRADVSRSDPPSFGSGTSSSSS--SSENFFKPET---LSCV 60
           MKLPK  + +PKRLF SKK+R D+SR DP SFGS TSSSSS  SS +  KP +    + +
Sbjct: 1   MKLPKSLKLSPKRLFGSKKNRPDLSRHDPLSFGSSTSSSSSSDSSASLKKPGSGTEFANI 60

Query: 61  GTPTSVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEE 120
           GTP SVLP  S DW ++SA+ + ELVQAFKL+DRDNDG+VSRKELEA+L R+GAEP ++E
Sbjct: 61  GTPKSVLPQVSGDWGEISAEKHMELVQAFKLMDRDNDGMVSRKELEALLSRLGAEPLSQE 120

Query: 121 EVAMMLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEEL 180
           EV MML++VD +G G IS++AL+ R+   +  GPAGD+EL ETF+FFD+DHDG+ITAEEL
Sbjct: 121 EVTMMLSDVDGEGHGCISVDALLTRV--GSECGPAGDTELRETFDFFDTDHDGKITAEEL 180

Query: 181 LSVFSSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR 219
           LSVF ++GDE CTL+DCRRMI  VD+NGDGFVCF +F RMM LQR
Sbjct: 181 LSVFQTIGDERCTLEDCRRMIDGVDKNGDGFVCFEDFSRMMELQR 223

[Close]

BLAST of Sgr017260 vs. ExPASy TrEMBL
Match: A0A7N2M177 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.7e-67
Identity = 144/225 (64.00%), Postives = 178/225 (79.11%), Query Frame = 0


Query: 1   MKLPK--RFTPKRLFRSKKSRADVSRSDPPSFGSGTSSSSS--SSENFFKPET---LSCV 60
           MKLPK  + +PKRLF SKK+R D+SR DP SFGS TSSSSS  SS +  KP +    + +
Sbjct: 1   MKLPKSLKLSPKRLFGSKKNRPDLSRHDPLSFGSSTSSSSSSDSSASLKKPGSGTEFANI 60

Query: 61  GTPTSVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEE 120
           GTP SVLP  S DW ++SA+   ELVQAFKL+DRDNDG+VSRKELEA+L R+GAEP ++E
Sbjct: 61  GTPKSVLPQVSGDWGEISAEKQLELVQAFKLMDRDNDGMVSRKELEALLSRLGAEPLSQE 120

Query: 121 EVAMMLNEVDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEEL 180
           EV MML++VD +G G IS++AL+ R+   +  GPAGD+EL ETF+FFD+DHDG+ITAEEL
Sbjct: 121 EVTMMLSDVDGEGHGCISVDALLTRV--GSECGPAGDTELRETFDFFDTDHDGKITAEEL 180

Query: 181 LSVFSSMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR 219
           LSVF ++GDE CTL+DCRRMI  VD+NGDGFVCF +F RMM LQR
Sbjct: 181 LSVFQTIGDERCTLEDCRRMIDGVDKNGDGFVCFEDFSRMMELQR 223

[Close]

BLAST of Sgr017260 vs. ExPASy TrEMBL
Match: A0A5N5GX95 (Calcium-binding protein CML36 OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_039425 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 6.2e-67
Identity = 141/217 (64.98%), Postives = 174/217 (80.18%), Query Frame = 0


Query: 4   PKRFTPKRLFRSKKSRADVSRSDPPSFGSGT-SSSSSSSENFFKPET-LSCVGTPTSVLP 63
           PK  +PKRLF SKK R+ VSRS+PPSFGSGT SSSSSSS++  KP      + TPTSVLP
Sbjct: 8   PKNLSPKRLFGSKKDRSSVSRSEPPSFGSGTDSSSSSSSDSIHKPRAGAGGITTPTSVLP 67

Query: 64  DSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVAMMLNE 123
           + S DWSD SAD   ++ QAFKLIDRDNDGVVSRKELEA+LCR+G +PP++EEV +ML+E
Sbjct: 68  EKSGDWSDFSAD--LDMAQAFKLIDRDNDGVVSRKELEALLCRLGNDPPSQEEVTLMLSE 127

Query: 124 VDDDGDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEELLSVFSSMG 183
           VD DG+G I+LEAL+ ++     SGPA DSEL + FE FD+D+DG+I+AEELL VF+++G
Sbjct: 128 VDRDGNGSITLEALLNQV--GPVSGPAADSELRDAFEVFDTDNDGKISAEELLRVFTAIG 187

Query: 184 DEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR 219
           DE CTL++CRRMI  VD++GDGFVCF EFV MM LQR
Sbjct: 188 DERCTLEECRRMIDGVDKDGDGFVCFEEFVHMMELQR 220

[Close]

BLAST of Sgr017260 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 205.3 bits (521), Expect = 5.0e-53
Identity = 126/220 (57.27%), Postives = 154/220 (70.00%), Query Frame = 0


Query: 1   MKLPKRFTPKRLFRSKKSRADVSRSDPP-SFGSGTSSSSSSSENFFKPETLSCVGTPTSV 60
           MKL K   PKR F   K R+ VS+S    SFGS +SSS    +N         V TPTS+
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSV-TPTSI 60

Query: 61  LPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVAMML 120
           LP+  + +S +      E++QAFKLIDRDNDG VSR +LE++L R+G +P TEEE+ +ML
Sbjct: 61  LPEVPSPYSYV------EILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVML 120

Query: 121 NEVDDDGDGFISLEALMARIVMSAASGPAGDS-ELLETFEFFDSDHDGRITAEELLSVFS 180
            EVD DGDG I LE L +R+V   +  PA DS EL ETFEFFD+D DG I+A+ELL VFS
Sbjct: 121 KEVDCDGDGTIRLEELASRVV---SLDPARDSTELKETFEFFDADRDGLISADELLRVFS 180

Query: 181 SMGDEGCTLDDCRRMIADVDENGDGFVCFAEFVRMMGLQR 219
           ++GDE CTLDDC+RMIADVDE+GDGFVCF EF RMM LQR
Sbjct: 181 TIGDERCTLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Sgr017260 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 192.2 bits (487), Expect = 4.4e-49
Identity = 112/209 (53.59%), Postives = 143/209 (68.42%), Query Frame = 0


Query: 6   RFTPKRLFRSKKSRADVSRSDPPSFGSGTSSSSSSSENFFKPETLSCVGTPTSVLPDSSA 65
           R +PKRLFR+ KS+A VSRS+P SF S  ++SSSSS+  +         TP SVLP +S 
Sbjct: 9   RLSPKRLFRT-KSKASVSRSEPSSFSS--NASSSSSDGSYGNLKQGPTATPISVLPQNSG 68

Query: 66  DWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVAMMLNEVDDD 125
           D+        +ELVQAFKLIDRD+DGVVSR +L A++ R+  EPP++EEV++ML EVD  
Sbjct: 69  DF-------YTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGG 128

Query: 126 GDGFISLEALMARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEELLSVFSSMGDEGC 185
             G ISLE L +R+  ++  G     EL E FE FD D +G+I+AEEL  VF  +GDE C
Sbjct: 129 DGGCISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERC 188

Query: 186 TLDDCRRMIADVDENGDGFVCFAEFVRMM 215
           TL++C RMIA VD NGDGFVCF +F RMM
Sbjct: 189 TLEECMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Sgr017260 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 99.0 bits (245), Expect = 5.0e-21
Identity = 59/144 (40.97%), Postives = 89/144 (61.81%), Query Frame = 0


Query: 74  SNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPP--TEEEVAMMLNEVDDDGDGFIS 133
           S  ++ + F+  D++NDG +S  EL+ +   IGA  P  ++EE   M+ E D DG+GFI 
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDV---IGALSPNASQEETKAMMKEFDLDGNGFID 71

Query: 134 LEALMARIVMS-AASGPAGDSELLETFEFFDSDHDGRITAEELLSVFSSMGDEGCTLDDC 193
           L+  +A   +S  +S  +   +L E F+ +D D +GRI+A EL SV  ++G E C++ DC
Sbjct: 72  LDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDC 131

Query: 194 RRMIADVDENGDGFVCFAEFVRMM 215
           +RMI  VD +GDG V F EF +MM
Sbjct: 132 QRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Sgr017260 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 98.2 bits (243), Expect = 8.6e-21
Identity = 58/165 (35.15%), Postives = 101/165 (61.21%), Query Frame = 0


Query: 55  TPTSVLPDSSADWSDLSADSNSELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEE 114
           +P+++LP  S+  +       SEL + F++ D++ DG ++++EL   L  +G   P +++
Sbjct: 43  SPSTMLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIP-DKD 102

Query: 115 VAMMLNEVDDDGDGFISL---EALMARIVMSAAS-GPAGDSELLETFEFFDSDHDGRITA 174
           +  M++++D +GDG + +   E+L + IV    + G   + ++ + F  FD D DG IT 
Sbjct: 103 LTQMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITV 162

Query: 175 EELLSVFSSMG-DEGCTLDDCRRMIADVDENGDGFVCFAEFVRMM 215
           EEL SV +S+G  +G TLD C++MI  VD +GDG V + EF++MM
Sbjct: 163 EELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206

[Close]

BLAST of Sgr017260 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 97.1 bits (240), Expect = 1.9e-20
Identity = 52/140 (37.14%), Postives = 87/140 (62.14%), Query Frame = 0


Query: 76  SELVQAFKLIDRDNDGVVSRKELEAILCRIGAEPPTEEEVAMMLNEVDDDGDGFISLEAL 135
           +EL + F++ DR+ DG ++++EL   L  +G   P ++++  M+ ++D +GDG++ +E  
Sbjct: 4   AELARIFQMFDRNGDGKITKQELNDSLENLGIYIP-DKDLVQMIEKIDLNGDGYVDIEE- 63

Query: 136 MARIVMSAASGPAGDSELLETFEFFDSDHDGRITAEELLSVFSSMG-DEGCTLDDCRRMI 195
              +  +       + ++ E F  FD + DG IT EEL SV +S+G  +G TL+DC+RMI
Sbjct: 64  FGGLYQTIMEERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMI 123

Query: 196 ADVDENGDGFVCFAEFVRMM 215
           + VD +GDG V F EF +MM
Sbjct: 124 SKVDVDGDGMVNFKEFKQMM 141

The following BLAST results are available for this feature:

BLAST of Sgr017260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153870.1	8.5e-71	71.04	probable calcium-binding protein CML36 [Momordica charantia]	[more]
KAF3947310.1	3.0e-68	63.60	hypothetical protein CMV_026538 [Castanea mollissima]	[more]
XP_024181636.1	3.3e-67	67.12	probable calcium-binding protein CML36 [Rosa chinensis] >PRQ49697.1 putative EF-...	[more]
XP_030949696.1	5.7e-67	64.00	probable calcium-binding protein CML36 [Quercus lobata]	[more]
KAF3963812.1	7.4e-67	63.35	hypothetical protein CMV_011844 [Castanea mollissima]	[more]

BLAST of Sgr017260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SS31	7.0e-52	57.27	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
P30188	6.2e-48	53.59	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q8RZB5	1.9e-20	37.42	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q5ZCK5	1.9e-20	43.92	Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9C8Y1	7.1e-20	40.97	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]

BLAST of Sgr017260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DI26	4.1e-71	71.04	probable calcium-binding protein CML36 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A2P6RTD5	1.6e-67	67.12	Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2...	[more]
A0A7N2RFF9	2.8e-67	64.00	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]
A0A7N2M177	4.7e-67	64.00	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]
A0A5N5GX95	6.2e-67	64.98	Calcium-binding protein CML36 OS=Pyrus ussuriensis x Pyrus communis OX=2448454 G...	[more]

BLAST of Sgr017260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10190.1	5.0e-53	57.27	Calcium-binding EF-hand family protein	[more]
AT2G41410.1	4.4e-49	53.59	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	5.0e-21	40.97	calmodulin like 23	[more]
AT2G43290.1	8.6e-21	35.15	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	1.9e-20	37.14	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 77..105 e-value: 0.0024 score: 27.1 coord: 152..180 e-value: 5.6E-5 score: 32.5 coord: 114..142 e-value: 0.13 score: 21.4 coord: 189..217 e-value: 3.6E-5 score: 33.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 77..133 e-value: 9.2E-10 score: 38.8 coord: 151..214 e-value: 2.8E-10 score: 40.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 110..145 score: 9.296966
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 185..218 score: 12.253878
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 148..183 score: 13.871811
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 73..108 score: 13.537066
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 77..139 e-value: 5.54517E-14 score: 62.5653
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 152..214 e-value: 3.96817E-16 score: 67.9581
None	No IPR available	GENE3D	1.10.238.10		coord: 143..218 e-value: 1.2E-19 score: 72.7
None	No IPR available	GENE3D	1.10.238.10		coord: 57..141 e-value: 4.0E-18 score: 67.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 25..47
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..47
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 4..215
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 4..215
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 86..98
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 198..210
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 161..173
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 64..214

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr017260.1	Sgr017260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding