Sgr015218 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr015218
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	O-methyltransferase family protein
Location	tig00003063: 846902 .. 848476 (-)
RNA-Seq Expression	Sgr015218
Synteny	Sgr015218

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACTTAGCCACAGAGAATCTAAATCGGAGAAGGAAGAAGATGGCCATGCCACTGTCCAGATATGGAAATACATATTGGGTTTCTCCGAATTGGCAGTAGCAAAATGTGCCATAGAGCTGAAAATTGCTGACGTTATTGAAAGTCATGGAACTCCCATGACATTGTCACAGTTGTCATCTGCTTTAAACTGCTCTTCGTCGCTCCTACACCGCATTTTGAGATTCCTCATCCATCGCGGAATTTTCAAAGAAGAAACCACAGCCGAAAACCTCACAGGCTATGCTCAAACACCTATGTCTCGCTTGCTTACTAGCAGCGGTGAAAACAGCATGGGTGCCTTTATTATGCTCGAGAATAGCCCGACGATGCTCGCACCATGGCATAACTTGAGTGCACACATCAAGGCCAAGGGCAATGACCCCTTACCATTTGAGGCTGCTCATGGCAAGGATTTGTGGCATTACGCTGCAGACAATCCAAAACACAATGCACTGTTCAACGAGGCCATGGCTTGTTCTGCTAGGATGGTCACTTTGCCTGCAATACTTGAAGGTTGTCGAGAGATTTTTAATGGGGTTGGATGTTTGGTGGATGTGGGAGGAGGAAATGGGACTAGCTTAAGCATGATAGTAAAGGCTTGCCCTTGGATTCAAGGTATCAACTTTGATCTTCAACATGTTGTGTCTGCCTCACCAGAACACAAGGGTGTGCAACATGTGGGTGGCAACATGTTCAACAATGTCCCCAAGGCTGATGTTGCTTTTCTCATGGTTTGTATATGTGTGTTTGTGAGTAAAATTACTACATGACTACTTTATGGTAACCTTCATTTGGTTCCTATATTTGTTAAAGTGCTTTAGGTGTAAAAGGGAATATGTATAAAATCGATAGGGTATGTGTGTTGAAAACATCTTTAAAAATAGTTTTCAAAACAGGAATCACAAAATATTGTGTTGTTTTTATGTATTTTCTTTTATAATTTGTTAAATTTACTATTTGTTAGGTATGACTTAATTCAAATGTATTATATTTTTAAATTAGTATGGAGAATAGATAAATGAGAACAAGGATATCTTTTCAAATAGTTTTTTAAATGAAAAACAATAGAAAATAGTTTTAACAGGTGAATTTATAAAATAATTTTTTTAAAAAATACACCTATATTGTCCTGTTTTACCATATAAGTGTTTCTCGTGCTTACTCTCTTAAATCCCATTACATAAGCTTTCTAGCAATTAATGTACAAATCAATGACTTTTGAAGTTAAATTGTAGTGGGTTCTACACGATTGGAATGATGAAGAATGCATCAAAATTTTGAAGAACTGCAAAGAAGCCACTCCAAATAACATAGGAAAAGTGATAATTATAGAAGCAGTGATTGATGAAAAAGAAGAAAGCAAGTTGTTAGATGTAGGGTTGATGCTCGACATGGTGATGATGGCCCATACCAACAAAGGCAAGGAAAGGACACGTGAAGAATGGGCCTATGTTATCAACACAGCTGGCTTTAGTCGATACACTATCACACCTATTCGAGCTGTCCAATCTGTTATTCAATGCTTCCCTTGA

mRNA sequence

ATGGAACTTAGCCACAGAGAATCTAAATCGGAGAAGGAAGAAGATGGCCATGCCACTGTCCAGATATGGAAATACATATTGGGTTTCTCCGAATTGGCAGTAGCAAAATGTGCCATAGAGCTGAAAATTGCTGACGTTATTGAAAGTCATGGAACTCCCATGACATTGTCACAGTTGTCATCTGCTTTAAACTGCTCTTCGTCGCTCCTACACCGCATTTTGAGATTCCTCATCCATCGCGGAATTTTCAAAGAAGAAACCACAGCCGAAAACCTCACAGGCTATGCTCAAACACCTATGTCTCGCTTGCTTACTAGCAGCGGTGAAAACAGCATGGGTGCCTTTATTATGCTCGAGAATAGCCCGACGATGCTCGCACCATGGCATAACTTGAGTGCACACATCAAGGCCAAGGGCAATGACCCCTTACCATTTGAGGCTGCTCATGGCAAGGATTTGTGGCATTACGCTGCAGACAATCCAAAACACAATGCACTGTTCAACGAGGCCATGGCTTGTTCTGCTAGGATGGTCACTTTGCCTGCAATACTTGAAGGTTGTCGAGAGATTTTTAATGGGGTTGGATGTTTGGTGGATGTGGGAGGAGGAAATGGGACTAGCTTAAGCATGATAGTAAAGGCTTGCCCTTGGATTCAAGGTATCAACTTTGATCTTCAACATGTTGTGTCTGCCTCACCAGAACACAAGGGTGTGCAACATGTGGGTGGCAACATGTTCAACAATGTCCCCAAGGCTGATGTTGCTTTTCTCATGTGGGTTCTACACGATTGGAATGATGAAGAATGCATCAAAATTTTGAAGAACTGCAAAGAAGCCACTCCAAATAACATAGGAAAAGTGATAATTATAGAAGCAGTGATTGATGAAAAAGAAGAAAGCAAGTTGTTAGATGTAGGGTTGATGCTCGACATGGTGATGATGGCCCATACCAACAAAGGCAAGGAAAGGACACGTGAAGAATGGGCCTATGTTATCAACACAGCTGGCTTTAGTCGATACACTATCACACCTATTCGAGCTGTCCAATCTGTTATTCAATGCTTCCCTTGA

Coding sequence (CDS)

ATGGAACTTAGCCACAGAGAATCTAAATCGGAGAAGGAAGAAGATGGCCATGCCACTGTCCAGATATGGAAATACATATTGGGTTTCTCCGAATTGGCAGTAGCAAAATGTGCCATAGAGCTGAAAATTGCTGACGTTATTGAAAGTCATGGAACTCCCATGACATTGTCACAGTTGTCATCTGCTTTAAACTGCTCTTCGTCGCTCCTACACCGCATTTTGAGATTCCTCATCCATCGCGGAATTTTCAAAGAAGAAACCACAGCCGAAAACCTCACAGGCTATGCTCAAACACCTATGTCTCGCTTGCTTACTAGCAGCGGTGAAAACAGCATGGGTGCCTTTATTATGCTCGAGAATAGCCCGACGATGCTCGCACCATGGCATAACTTGAGTGCACACATCAAGGCCAAGGGCAATGACCCCTTACCATTTGAGGCTGCTCATGGCAAGGATTTGTGGCATTACGCTGCAGACAATCCAAAACACAATGCACTGTTCAACGAGGCCATGGCTTGTTCTGCTAGGATGGTCACTTTGCCTGCAATACTTGAAGGTTGTCGAGAGATTTTTAATGGGGTTGGATGTTTGGTGGATGTGGGAGGAGGAAATGGGACTAGCTTAAGCATGATAGTAAAGGCTTGCCCTTGGATTCAAGGTATCAACTTTGATCTTCAACATGTTGTGTCTGCCTCACCAGAACACAAGGGTGTGCAACATGTGGGTGGCAACATGTTCAACAATGTCCCCAAGGCTGATGTTGCTTTTCTCATGTGGGTTCTACACGATTGGAATGATGAAGAATGCATCAAAATTTTGAAGAACTGCAAAGAAGCCACTCCAAATAACATAGGAAAAGTGATAATTATAGAAGCAGTGATTGATGAAAAAGAAGAAAGCAAGTTGTTAGATGTAGGGTTGATGCTCGACATGGTGATGATGGCCCATACCAACAAAGGCAAGGAAAGGACACGTGAAGAATGGGCCTATGTTATCAACACAGCTGGCTTTAGTCGATACACTATCACACCTATTCGAGCTGTCCAATCTGTTATTCAATGCTTCCCTTGA

Protein sequence

MELSHRESKSEKEEDGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQLSSALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLENSPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTLPAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQHVGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEESKLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCFP

Homology

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BLAST of Sgr015218 vs. NCBI nr
Match: XP_022135713.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 548.1 bits (1411), Expect = 5.5e-152
Identity = 266/356 (74.72%), Postives = 306/356 (85.96%), Query Frame = 0


Query: 1   MELSHRESKSEKEED-GHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQL 60
           M+L+ +ES S+KEED  HA VQ+W YI GF+E+A+ KCAIELKIAD IESHG+PMTLS+L
Sbjct: 11  MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70

Query: 61  SSALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLE 120
           SSALNCS  LL+RILRFL HRGIFKEETTA+NLTGYAQTP+SRLL S+  NSM  F++LE
Sbjct: 71  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130

Query: 121 NSPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVT 180
           ++P MLAPWHNLSA IKAK  D  PFEA HGKDLW Y A +P HN +FN+AMAC+AR++T
Sbjct: 131 SNPVMLAPWHNLSALIKAK--DGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVIT 190

Query: 181 LPAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQ 240
           +PAILEGC EIF GVG LVDVGGGNGTSLSMIVKACPWIQGINFDL HVVS + E  GVQ
Sbjct: 191 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQ 250

Query: 241 HVGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEE 300
           HV GNMF+ +PKAD AF+MWVLHDWNDE+CIKILKNC++A P  IGKVII+EAVID  EE
Sbjct: 251 HVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEE 310

Query: 301 SKLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCF 356
           + L DVGL+LDMVMMAHTNKGKERT EEWAYV++ AGF+RYTITP++AVQSVIQ F
Sbjct: 311 NNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 364

[Close]

BLAST of Sgr015218 vs. NCBI nr
Match: KAG6572139.1 (Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 532.3 bits (1370), Expect = 3.1e-147
Identity = 252/356 (70.79%), Postives = 302/356 (84.83%), Query Frame = 0


Query: 1   MELSHRESKSEKEEDGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQLS 60
           M+ + ++S S+KEE  HA +Q+W+YI GF E+A+ KCAIELKI D I+SHG  MT+ +LS
Sbjct: 9   MDFALKQSISKKEEHEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTIPELS 68

Query: 61  SALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLEN 120
           S+LNCSSSLLHRI+RFL+HRGIFK+E T ENLT Y+ TP+SRLLTS  ENSM  F++LE+
Sbjct: 69  SSLNCSSSLLHRIMRFLVHRGIFKQEITGENLTRYSHTPLSRLLTSGSENSMAPFVLLES 128

Query: 121 SPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTL 180
           +P MLAPWH+LSA I  KGN+ +PFEAAHGKDLW YAA +P HN  FN+AMACSAR++++
Sbjct: 129 NPVMLAPWHSLSARI--KGNEAIPFEAAHGKDLWSYAAADPIHNVTFNDAMACSARVMSV 188

Query: 181 PAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQH 240
           PAILE C EIF GV CLVDVGGGNGTSLSMIVKACPWI+ INFDL HV+++ P   GVQH
Sbjct: 189 PAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVIASLPADIGVQH 248

Query: 241 VGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEES 300
           VGGNMF+++PKAD AFLMWVLHDW+DE CIKILKNC+EA P   GKVII+EAV++EKEE+
Sbjct: 249 VGGNMFDSIPKADAAFLMWVLHDWDDETCIKILKNCREAIPKKTGKVIIVEAVLEEKEEN 308

Query: 301 KLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCFP 357
            L DVGLMLDMVMMAHTN GKERT +EWAYV++ AGF+R+TITPIRAVQSVIQ FP
Sbjct: 309 NLSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRAVQSVIQAFP 362

[Close]

BLAST of Sgr015218 vs. NCBI nr
Match: XP_022136086.1 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica charantia])

HSP 1 Score: 530.4 bits (1365), Expect = 1.2e-146
Identity = 262/356 (73.60%), Postives = 299/356 (83.99%), Query Frame = 0


Query: 1   MELSHRESKSEKEED-GHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQL 60
           M+L+ +ES S+KEED   A VQ+W YI GF+++A+ KCAIELKIAD IESHG+PMTLSQL
Sbjct: 1   MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60

Query: 61  SSALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLE 120
           SSALNCS  LL+RILRFL HRGIFKEETTA+NLTGYAQTP+SRLL S+G NSM   ++LE
Sbjct: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120

Query: 121 NSPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVT 180
           +SP MLA WHNLSA IKA  +D  PFEA HGKDLW Y A +P HN +FN+AMAC+AR++T
Sbjct: 121 SSPIMLASWHNLSARIKA--SDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVIT 180

Query: 181 LPAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQ 240
           +PAILEGC EIF GVG LVDVGGGNGTSLSMIVKACPWI+GINFDL HVVS + E  GVQ
Sbjct: 181 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQ 240

Query: 241 HVGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEE 300
           HV GNMF+ +PKAD AFLM VLHDWNDE+CIKILKNCK+A P   GKVII+E VID  EE
Sbjct: 241 HVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEE 300

Query: 301 SKLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCF 356
             L DVGLMLDMVMMA TNKGKERT EEWA+V++ +GFSRYTITPIRA+QSVIQ F
Sbjct: 301 KNLSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAF 354

[Close]

BLAST of Sgr015218 vs. NCBI nr
Match: XP_023554119.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 529.6 bits (1363), Expect = 2.0e-146
Identity = 250/356 (70.22%), Postives = 303/356 (85.11%), Query Frame = 0


Query: 1   MELSHRESKSEKEEDGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQLS 60
           M+ + ++S S+KEE  HA +Q+W+YI GF E+A+ KCAIELKI D I+SHG  MTL +LS
Sbjct: 1   MDFALKQSISKKEEHEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPELS 60

Query: 61  SALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLEN 120
           S+LNCSSSLLHRI+RFL+HRGIFK+E T ENLT Y+ TP+SRLLTS  ENSM  F++LE+
Sbjct: 61  SSLNCSSSLLHRIMRFLVHRGIFKQEITGENLTRYSHTPLSRLLTSGSENSMAPFVLLES 120

Query: 121 SPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTL 180
           +P MLAPWH+LSA I  KGN+ +PFEAAHGKD+W YAA +P HNA+FN+AMACSAR++++
Sbjct: 121 NPVMLAPWHSLSARI--KGNEAIPFEAAHGKDIWSYAAADPIHNAMFNDAMACSARVMSV 180

Query: 181 PAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQH 240
           PAILE C EIF GV CLVDVGGGNGTSLSMIVKACPWI+GINFDL HV+++ P   GVQH
Sbjct: 181 PAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVIASLPADIGVQH 240

Query: 241 VGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEES 300
           VGGNMF+ +PKAD AFLMWVLHDW+DE CIKILKNC++A P   GKVII+EAV++ K+E+
Sbjct: 241 VGGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQEN 300

Query: 301 KLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCFP 357
            L DVGLMLDMVMMAHTN GKERT +EWAYV++ AGF+R+TITPIRA+QSVIQ FP
Sbjct: 301 NLSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAFP 354

[Close]

BLAST of Sgr015218 vs. NCBI nr
Match: XP_022135685.1 (desmethylxanthohumol 6'-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 529.6 bits (1363), Expect = 2.0e-146
Identity = 259/356 (72.75%), Postives = 298/356 (83.71%), Query Frame = 0


Query: 1   MELSHRESKSEKEE-DGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQL 60
           ME SH+ESK +KEE D  AT+QIWK+I GFSE+AV KCAIELKI D IE +G+PMTLSQL
Sbjct: 1   MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 60

Query: 61  SSALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLE 120
           SSALNCSS LL+RILRFLI RGIF+EETT ENLT YAQTP+SRLLT+SGE+SM    +LE
Sbjct: 61  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 120

Query: 121 NSPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVT 180
           N P MLAPWHNLSA+IK   ++ LPFEAAHG DLW YAA NP  NALFNEA+AC  R++T
Sbjct: 121 NGPVMLAPWHNLSANIK-NNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVIT 180

Query: 181 LPAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQ 240
           +PAILE C E+F GVGCLVDVGGGNGTSLSMIVKACPWI+G+NFDL HVVS SPE++ V+
Sbjct: 181 VPAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVR 240

Query: 241 HVGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEE 300
           HVGGNMF+ VPKAD AF MW+LH W+DEECIKILKNCKEA P + GKVII+E VIDEKE+
Sbjct: 241 HVGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEK 300

Query: 301 SKLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCF 356
           SK  D+ LMLDM MMA   KGKERT +EWAY++N AGFSR+TITPI  + S+IQ F
Sbjct: 301 SKTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 355

[Close]

BLAST of Sgr015218 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 3.3e-99
Identity = 179/355 (50.42%), Postives = 250/355 (70.42%), Query Frame = 0


Query: 13  EEDGHATVQIWKYILGFSELAVAKCAIELKIADVIESH--GTPMTLSQLSSALNCSSSLL 72
           +E+  A++ IWKY+ GF+++A AKCAI+LKI + IE+H    P+TL++LSSA++ S S L
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 73  HRILRFLIHRGIFKEETTAENL-TGYAQTPMSR--LLTSSGENSMGAFIMLENSPTMLAP 132
            RI+RFL+H+GIFKE  T + L TGY  TP+SR  ++T     S+  F++ E +P MLAP
Sbjct: 84  RRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAP 143

Query: 133 WHNLSAHIKA--KGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTLPAILE 192
           W  LS+ + +   G+ P PF+A HGKD+W +A DNP  + + NEAMAC AR V +P +  
Sbjct: 144 WLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRV-VPRVAG 203

Query: 193 GCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQHVGGNM 252
            C  +F+GV  +VDVGGG G ++ M+VK  PWI+G NFDL HV+  +    GV++V G+M
Sbjct: 204 ACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEGDM 263

Query: 253 FNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEESKLLD- 312
           F+++P  D  F+ WVLHDW D++CIKILKNCKEA P NIGKV+I+E+VI E +++ ++D 
Sbjct: 264 FDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTMIVDE 323

Query: 313 -------VGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVI 353
                  V LMLDMVMMAHT+ GKERT +EW +V+  AGF+RY +  I  VQS+I
Sbjct: 324 RDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

[Close]

BLAST of Sgr015218 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 6.9e-73
Identity = 158/367 (43.05%), Postives = 224/367 (61.04%), Query Frame = 0


Query: 1   MELSHRESKSEKEEDGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQLS 60
           MEL+ R  ++E    G A V  WK I G ++  V KCA+EL+I D++ SH  P+TL+Q++
Sbjct: 1   MELA-RNDQTEAALRGEANV--WKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIA 60

Query: 61  SALNCSSSL----LHRILRFLIHRGIFKEETTAE-NLTGYAQTPMSRLLTSS----GENS 120
           S++  S SL    L RI+R L+ R IF +  + +     Y  T  SRLL S      + +
Sbjct: 61  SSVPDSPSLNLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVT 120

Query: 121 MGAFIMLENSPTMLAPWHNLSAHIKAKGNDPLPFEAAH-GKDLWHYAADNPKHNALFNEA 180
           +  F+     P + APW  L+  +K  GN    FE  H G+ LW  +  NP+ N +FN+ 
Sbjct: 121 LAPFVAFMTHPYLSAPWSCLARCVKEGGNG---FEMVHGGRQLWDLSPGNPEFNKVFNDG 180

Query: 181 MACSARMVTLPAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVS 240
           MA +AR+ T+ AIL   R++F G+  LVDVGG  G S+S IVK+ P I+GIN+DL HVV+
Sbjct: 181 MASTARITTM-AILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVA 240

Query: 241 ASPEHKG-VQHVGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVII 300
            +P + G V HVGGNMF  +P A   F+ W+LHDW DE+C+KILKNC+ A P   GK+II
Sbjct: 241 TAPTYTGLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIII 300

Query: 301 IEAVIDEKEESKLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQ 357
           ++ V++ +      D  +MLD+ +MA T +GKERT +EW  V+   GF RY I  I A+ 
Sbjct: 301 VDIVLEPEGNGLFDDAAVMLDIALMALT-RGKERTEKEWKRVLEEGGFPRYQILKIPALT 359

[Close]

BLAST of Sgr015218 vs. ExPASy Swiss-Prot
Match: Q6WUC1 ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.6e-72
Identity = 149/347 (42.94%), Postives = 212/347 (61.10%), Query Frame = 0


Query: 15  DGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQLSSALNCSSSL----L 74
           D     +IWK I GF+E  V KCA++L+IA+ + ++  PM+LS+L+S L  +  +    L
Sbjct: 8   DQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNEDRL 67

Query: 75  HRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLENSPTMLAPWHN 134
            RI+R+L+H  +FK + T +    Y+  P ++ L    E SM   I+  N    LAPWH+
Sbjct: 68  FRIMRYLVHMELFKIDATTQK---YSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWHH 127

Query: 135 LSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTLPAILEGCREI 194
           L   +    +    FE A GK +W Y + NP+ N LFN AMAC  R+VT  A+   C+ I
Sbjct: 128 LGDGLTGNCD---AFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVT-SALANECKSI 187

Query: 195 F-NGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQHVGGNMFNNV 254
           F +G+  LVDVGGG GT++  I KA P I+   +DL HV++ SPE   +  + G+MF ++
Sbjct: 188 FSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 247

Query: 255 PKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEESKLLDVGLML 314
           P AD  F+  +LHDWND+ECI+ILK CKEA P   GKVII++ VID         + L L
Sbjct: 248 PSADAIFMKCILHDWNDDECIQILKRCKEALPKG-GKVIIVDVVIDMDSTHPYAKIRLTL 307

Query: 315 DMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCFP 357
           D+ MM +T  GKERT+EEW  + + AGF+ + +T I AVQSVI+ +P
Sbjct: 308 DLDMMLNTG-GKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345

[Close]

BLAST of Sgr015218 vs. ExPASy Swiss-Prot
Match: Q5C9L7 ((RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 8.5e-71
Identity = 141/359 (39.28%), Postives = 217/359 (60.45%), Query Frame = 0


Query: 1   MELSHRESKSEKEEDGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQLS 60
           ME+ ++E+ S +        ++W +I GF++  V K A++L +A++I +HG+PMTLS+LS
Sbjct: 1   MEMINKENLSSQ-------AKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELS 60

Query: 61  SAL---NCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIM 120
             L     +   L+R+LR+L+H  +F + +    L  Y   P ++ L    +  M   I+
Sbjct: 61  LHLPSQPVNQDALYRVLRYLVHMKLFTKSSIDGELR-YGLAPPAKFLVKGWDKCMLGAIL 120

Query: 121 LENSPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARM 180
                  +APWH L   I   G+    FE A G ++W Y A++P+ N LFNE MA   R+
Sbjct: 121 TITDKDFMAPWHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRL 180

Query: 181 VTLPAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKG 240
           + + A+++ C  +F+G+  +VDVGGG GT++  I KA P I+   +DL HV++ SP +  
Sbjct: 181 I-MSALVKECSSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTE 240

Query: 241 VQHVGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEK 300
           +  + G+MF  +P AD   +  +LHDW+D+ECI+ILK CK+A P + GKVIII+ ++D K
Sbjct: 241 INSIQGDMFKYIPNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVK 300

Query: 301 EESKLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCFP 357
            E     + L LD+ MM +T  GKERT EEW  +I+ AG+  Y IT I AVQSVI+ +P
Sbjct: 301 SEHPYTKMRLTLDLDMMLNTG-GKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYP 349

[Close]

BLAST of Sgr015218 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.2e-70
Identity = 143/354 (40.40%), Postives = 206/354 (58.19%), Query Frame = 0


Query: 13  EEDGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQLSSALNCSS----- 72
           EE      +IW+++  F +    KCA+EL I D+I SHG P+T+S++  +L  ++     
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 73  --SLLHRILRFLIHRGIFKEETTAE-NLTGYAQTPMSRLLTSSGENSMGAFIMLENSPTM 132
               L RI+R L+H+ +F  E   E N   Y  T  S+ L    + ++   ++ E +P +
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 133 LAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTLPAIL 192
           L PW  L    + K +   PFE AHG ++W  A  +PK N   N AM CS   +    +L
Sbjct: 125 LKPWQYLGKCAQEKSS---PFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLL 184

Query: 193 EGCREIFNGV-GCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQHVGG 252
           E  ++ F+G+ G LVDVGGG G+ ++ IVKA P IQGINFDL HVV+ + E  GV+HVGG
Sbjct: 185 E-YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGG 244

Query: 253 NMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEA-TPNNIGKVIIIEAVIDEKEESKL 312
           +MF ++P+AD   + W+LHDW+DE+C  ILKNC  A      GKVII++ V+        
Sbjct: 245 DMFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLF 304

Query: 313 LDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCFP 357
             +GL+ D++MMAHT  GKERT  EW  ++N AGF RY +    A   +I+ FP
Sbjct: 305 DKMGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

[Close]

BLAST of Sgr015218 vs. ExPASy TrEMBL
Match: A0A6J1C5N1 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007607 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.7e-152
Identity = 266/356 (74.72%), Postives = 306/356 (85.96%), Query Frame = 0


Query: 1   MELSHRESKSEKEED-GHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQL 60
           M+L+ +ES S+KEED  HA VQ+W YI GF+E+A+ KCAIELKIAD IESHG+PMTLS+L
Sbjct: 11  MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70

Query: 61  SSALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLE 120
           SSALNCS  LL+RILRFL HRGIFKEETTA+NLTGYAQTP+SRLL S+  NSM  F++LE
Sbjct: 71  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130

Query: 121 NSPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVT 180
           ++P MLAPWHNLSA IKAK  D  PFEA HGKDLW Y A +P HN +FN+AMAC+AR++T
Sbjct: 131 SNPVMLAPWHNLSALIKAK--DGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVIT 190

Query: 181 LPAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQ 240
           +PAILEGC EIF GVG LVDVGGGNGTSLSMIVKACPWIQGINFDL HVVS + E  GVQ
Sbjct: 191 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQ 250

Query: 241 HVGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEE 300
           HV GNMF+ +PKAD AF+MWVLHDWNDE+CIKILKNC++A P  IGKVII+EAVID  EE
Sbjct: 251 HVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEE 310

Query: 301 SKLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCF 356
           + L DVGL+LDMVMMAHTNKGKERT EEWAYV++ AGF+RYTITP++AVQSVIQ F
Sbjct: 311 NNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 364

[Close]

BLAST of Sgr015218 vs. ExPASy TrEMBL
Match: A0A6J1C2I8 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111007866 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 5.8e-147
Identity = 262/356 (73.60%), Postives = 299/356 (83.99%), Query Frame = 0


Query: 1   MELSHRESKSEKEED-GHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQL 60
           M+L+ +ES S+KEED   A VQ+W YI GF+++A+ KCAIELKIAD IESHG+PMTLSQL
Sbjct: 1   MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60

Query: 61  SSALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLE 120
           SSALNCS  LL+RILRFL HRGIFKEETTA+NLTGYAQTP+SRLL S+G NSM   ++LE
Sbjct: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120

Query: 121 NSPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVT 180
           +SP MLA WHNLSA IKA  +D  PFEA HGKDLW Y A +P HN +FN+AMAC+AR++T
Sbjct: 121 SSPIMLASWHNLSARIKA--SDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVIT 180

Query: 181 LPAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQ 240
           +PAILEGC EIF GVG LVDVGGGNGTSLSMIVKACPWI+GINFDL HVVS + E  GVQ
Sbjct: 181 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQ 240

Query: 241 HVGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEE 300
           HV GNMF+ +PKAD AFLM VLHDWNDE+CIKILKNCK+A P   GKVII+E VID  EE
Sbjct: 241 HVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEE 300

Query: 301 SKLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCF 356
             L DVGLMLDMVMMA TNKGKERT EEWA+V++ +GFSRYTITPIRA+QSVIQ F
Sbjct: 301 KNLSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAF 354

[Close]

BLAST of Sgr015218 vs. ExPASy TrEMBL
Match: A0A6J1C1Q8 (desmethylxanthohumol 6'-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007583 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 9.8e-147
Identity = 259/356 (72.75%), Postives = 298/356 (83.71%), Query Frame = 0


Query: 1   MELSHRESKSEKEE-DGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQL 60
           ME SH+ESK +KEE D  AT+QIWK+I GFSE+AV KCAIELKI D IE +G+PMTLSQL
Sbjct: 1   MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 60

Query: 61  SSALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLE 120
           SSALNCSS LL+RILRFLI RGIF+EETT ENLT YAQTP+SRLLT+SGE+SM    +LE
Sbjct: 61  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 120

Query: 121 NSPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVT 180
           N P MLAPWHNLSA+IK   ++ LPFEAAHG DLW YAA NP  NALFNEA+AC  R++T
Sbjct: 121 NGPVMLAPWHNLSANIK-NNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVIT 180

Query: 181 LPAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQ 240
           +PAILE C E+F GVGCLVDVGGGNGTSLSMIVKACPWI+G+NFDL HVVS SPE++ V+
Sbjct: 181 VPAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVR 240

Query: 241 HVGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEE 300
           HVGGNMF+ VPKAD AF MW+LH W+DEECIKILKNCKEA P + GKVII+E VIDEKE+
Sbjct: 241 HVGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEK 300

Query: 301 SKLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCF 356
           SK  D+ LMLDM MMA   KGKERT +EWAY++N AGFSR+TITPI  + S+IQ F
Sbjct: 301 SKTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 355

[Close]

BLAST of Sgr015218 vs. ExPASy TrEMBL
Match: A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.3e-146
Identity = 253/356 (71.07%), Postives = 302/356 (84.83%), Query Frame = 0


Query: 1   MELSHRESKSEKEEDGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQLS 60
           M+ + ++S S+KEE  HA +Q+W+YI GF E+A+ KCAIELKI D I+SHG  MTL +LS
Sbjct: 1   MDFALKQSISKKEEHEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPELS 60

Query: 61  SALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLEN 120
           S+LNCSSSLL+RI+RFL+HRGIFKEE T ENLT Y+ TP+SRLL SS ++SM   ++LE+
Sbjct: 61  SSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLES 120

Query: 121 SPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTL 180
           +P MLAPWH LSA I  KGN+ +PFEAAHGKD+W YAA NP HNA+FN+AMACSAR++T+
Sbjct: 121 NPVMLAPWHGLSARI--KGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTV 180

Query: 181 PAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQH 240
           PAILE C EIF GV CLVDVGGGNGTSLSMIVKACPWI+GINFDL HVV++SP + GVQH
Sbjct: 181 PAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQH 240

Query: 241 VGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEES 300
           VGGNMF+ +PKAD AFLMWVLH W+DE CIKILK C+EA P   GKVII+EAV++EKEE+
Sbjct: 241 VGGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEEN 300

Query: 301 KLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCFP 357
            L DVGLMLDMVMMAHTN GKERT EEWAYV++ AGF+R+TITPIRA+QSVIQ FP
Sbjct: 301 NLSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAFP 354

[Close]

BLAST of Sgr015218 vs. ExPASy TrEMBL
Match: A0A6J1C6X0 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007930 PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 4.9e-146
Identity = 260/356 (73.03%), Postives = 299/356 (83.99%), Query Frame = 0


Query: 1   MELSHRESKSEKEE-DGHATVQIWKYILGFSELAVAKCAIELKIADVIESHGTPMTLSQL 60
           ME SH+ESK +KEE D  AT+QIWK+I GFSE+AV KCAIELKI D IE +G+PMTLSQL
Sbjct: 1   MENSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 60

Query: 61  SSALNCSSSLLHRILRFLIHRGIFKEETTAENLTGYAQTPMSRLLTSSGENSMGAFIMLE 120
           SSALNCSS LL+RILRFLI RGIF+EETT ENLT YAQTP+SRLLT+SGE+S+    +LE
Sbjct: 61  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSVAPLFLLE 120

Query: 121 NSPTMLAPWHNLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVT 180
           N P MLAPWHNL A IK   +D LPFEAAHG DLW YAA NP +N LF+EAMAC AR++ 
Sbjct: 121 NGPAMLAPWHNLCAKIKGV-SDVLPFEAAHGSDLWGYAAANPTYNTLFHEAMACGARLIH 180

Query: 181 LPAILEGCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQ 240
           +PAILE C E+F GVGCLVDVGGGNGTSLSMIVKACPWI+GINFDL HVV+ASPE++ VQ
Sbjct: 181 VPAILETCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVAASPEYENVQ 240

Query: 241 HVGGNMFNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEE 300
           HVGG+MFN VPKAD AFL W+LHDW+DEECIKILKNCKEA P + GKVII+EAVIDEKEE
Sbjct: 241 HVGGSMFNYVPKADAAFLKWILHDWDDEECIKILKNCKEAIPKDTGKVIIVEAVIDEKEE 300

Query: 301 SKLLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQCF 356
           S++ DV +M+DMV+MAHT  G+ERT EE A+V+N AGFSR+TI  I  V SVIQ F
Sbjct: 301 SEVSDVRVMVDMVVMAHTKNGRERTAEELAHVLNKAGFSRHTIISIGGVPSVIQAF 355

[Close]

BLAST of Sgr015218 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 363.2 bits (931), Expect = 2.3e-100
Identity = 179/355 (50.42%), Postives = 250/355 (70.42%), Query Frame = 0


Query: 13  EEDGHATVQIWKYILGFSELAVAKCAIELKIADVIESH--GTPMTLSQLSSALNCSSSLL 72
           +E+  A++ IWKY+ GF+++A AKCAI+LKI + IE+H    P+TL++LSSA++ S S L
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 73  HRILRFLIHRGIFKEETTAENL-TGYAQTPMSR--LLTSSGENSMGAFIMLENSPTMLAP 132
            RI+RFL+H+GIFKE  T + L TGY  TP+SR  ++T     S+  F++ E +P MLAP
Sbjct: 84  RRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAP 143

Query: 133 WHNLSAHIKA--KGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTLPAILE 192
           W  LS+ + +   G+ P PF+A HGKD+W +A DNP  + + NEAMAC AR V +P +  
Sbjct: 144 WLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRV-VPRVAG 203

Query: 193 GCREIFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQHVGGNM 252
            C  +F+GV  +VDVGGG G ++ M+VK  PWI+G NFDL HV+  +    GV++V G+M
Sbjct: 204 ACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEGDM 263

Query: 253 FNNVPKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEESKLLD- 312
           F+++P  D  F+ WVLHDW D++CIKILKNCKEA P NIGKV+I+E+VI E +++ ++D 
Sbjct: 264 FDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTMIVDE 323

Query: 313 -------VGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVI 353
                  V LMLDMVMMAHT+ GKERT +EW +V+  AGF+RY +  I  VQS+I
Sbjct: 324 RDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

[Close]

BLAST of Sgr015218 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 305.8 bits (782), Expect = 4.4e-83
Identity = 159/351 (45.30%), Postives = 218/351 (62.11%), Query Frame = 0


Query: 13  EEDGHATVQIWKYILGFSELAVAKCAIELKIADVIESH--GTPMTLSQLSSALNCSSSLL 72
           +E+  A++ IW+Y+ GF+++A AKCAI+LKI + IE+H    P+TLS+LSSA++ S S L
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHL 69

Query: 73  HRILRFLIHRGIFKEETTAENL-TGYAQTPMSRLLTSSGENSMGAFIMLENSPTMLAPWH 132
            RI+RFL+H+G+FKE  T + L TGY  TP+SR +                         
Sbjct: 70  RRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM------------------------ 129

Query: 133 NLSAHIKAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTLPAILEGCRE 192
                              HGKDLW +A DN  H+ L NEAMAC AR V +P +   C+ 
Sbjct: 130 ---------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPRVAGACQG 189

Query: 193 IFNGVGCLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQHVGGNMFNNV 252
           +F+GV  +VDVGGG G ++ ++VK  PWI+G NFDL HV+  +    GV++V G+MF+++
Sbjct: 190 LFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSI 249

Query: 253 PKADVAFLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKE--------ESK 312
           P +D   + WVLHDW D++CIKILKNCKEA   NIGKV+I+E VI EK+        + K
Sbjct: 250 PASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDK 309

Query: 313 LLDVGLMLDMVMMAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVI 353
           L  V L LDMVMM HT+ GKERT +EW +V+  AGF+RY +     VQS+I
Sbjct: 310 LEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 320

[Close]

BLAST of Sgr015218 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 199.1 bits (505), Expect = 5.8e-51
Identity = 112/314 (35.67%), Postives = 180/314 (57.32%), Query Frame = 0


Query: 36  KCAIELKIADVIESHGTPMTLSQLSSALNCSSS----LLHRILRFLIHRGIFKEETTAEN 95
           K A+EL + +++  +G+PM+ ++++S L   +     +L RILR L    +     +   
Sbjct: 37  KSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVL--TCSNRK 96

Query: 96  LTG------YAQTPMSRLLTSSGEN-SMGAFIMLENSPTMLAPWHNLSAHIKAKGNDPLP 155
           L+G      Y   P+ + LT + +  S+ A  ++     ++  W++L   I   G   +P
Sbjct: 97  LSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGG---IP 156

Query: 156 FEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTLPAILEGCREIFNGVGCLVDVGGGN 215
           F  A+G   + Y   +P+ N +FN  M+ +   +T+  ILE  +  F G+  LVDVGGG 
Sbjct: 157 FNKAYGMSAFEYHGTDPRFNKVFNNGMS-NHSTITMKKILETYKG-FEGLTSLVDVGGGI 216

Query: 216 GTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQHVGGNMFNNVPKADVAFLMWVLHDW 275
           G +L MIV   P ++GINFDL HV+  +P H G++HVGG+MF +VPK D  F+ W+ HDW
Sbjct: 217 GATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDW 276

Query: 276 NDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEESKLLDVGLM-LDMVMMAHTNKGKER 335
           +DE C+K LKNC E+ P + GKVI+ E ++ E  +S L    ++ +D +M+AH   GKER
Sbjct: 277 SDEHCVKFLKNCYESLPED-GKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKER 336

Query: 336 TREEWAYVINTAGF 338
           T +E+  +   +GF
Sbjct: 337 TEKEFEALAKASGF 342

[Close]

BLAST of Sgr015218 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 181.8 bits (460), Expect = 9.7e-46
Identity = 123/340 (36.18%), Postives = 188/340 (55.29%), Query Frame = 0


Query: 34  VAKCAIELKIADVI--ESHGTPMTLSQLSSALNCSSS------LLHRILRFLIHRGIFK- 93
           V K A+EL + D I   S+GT ++ S+++ +L    +      LL R+LR L+   I K 
Sbjct: 45  VLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILKC 104

Query: 94  --EETTAENLTG-----YAQTPMSR--LLTSSGENSMGAFIMLENSPTMLAPWHNLSAHI 153
              E+     TG     YA  P+ +  L  S G  S+ + ++L +S  +L  W NL   +
Sbjct: 105 CMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSSLLLLLHSQVILKTWTNLK-DV 164

Query: 154 KAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTLPAILEGCREIFNGVG 213
             +G D   F +AH   L+ Y + + + + LF+ AM+ S+ MV +  +LE  R  F  V 
Sbjct: 165 ILEGKD--AFSSAHDMRLFEYISSDDQFSKLFHRAMSESSTMV-MKKVLEEYRG-FEDVN 224

Query: 214 CLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQHVGGNMFNNVPKADVA 273
            LVDVGGG GT L +I    P I+G+NFDL  V++ +P + GV+HV G+MF  VPK D  
Sbjct: 225 TLVDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQAPFYPGVKHVSGDMFIEVPKGDAI 284

Query: 274 FLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAV--IDEKEESKLLDVGLMLDMVM 333
           F+ W+LHDW DE+CIKILKNC ++ P   GKVII+E +  ++ K      +  L +D++M
Sbjct: 285 FMKWILHDWGDEDCIKILKNCWKSLPEK-GKVIIVEMITPMEPKPNDFSCNTVLGMDLLM 344

Query: 334 MAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQ 354
           +   + GKER+  ++  +   +GF    I  +    SVI+
Sbjct: 345 LTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYSVIE 378

[Close]

BLAST of Sgr015218 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 176.8 bits (447), Expect = 3.1e-44
Identity = 119/340 (35.00%), Postives = 175/340 (51.47%), Query Frame = 0


Query: 34  VAKCAIELKIADVIESHGTPMTLSQLSSALNCSSS--------LLHRILRFLIHRGIFK- 93
           V K A EL + D I + G    LS    A +  +         LL R+L  L+   I K 
Sbjct: 45  VLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVSHSILKC 104

Query: 94  --EETTAENLTG-----YAQTPMSR--LLTSSGENSMGAFIMLENSPTMLAPWHNLSAHI 153
              ET     TG     YA  P+ +  L  S G  S+    ML ++      W NL   +
Sbjct: 105 RMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQVFFKTWTNLK-DV 164

Query: 154 KAKGNDPLPFEAAHGKDLWHYAADNPKHNALFNEAMACSARMVTLPAILEGCREIFNGVG 213
             +G D   F +AHG  ++ Y   +     LFN AM+  + M+ +  +L+  R  F  V 
Sbjct: 165 ILEGRD--AFNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMI-MKKVLDVYRG-FEDVN 224

Query: 214 CLVDVGGGNGTSLSMIVKACPWIQGINFDLQHVVSASPEHKGVQHVGGNMFNNVPKADVA 273
            LVDVGGGNGT L ++    P I+G+NFDL  V++ +P + GV+HV G+MF  VPK D  
Sbjct: 225 TLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDAV 284

Query: 274 FLMWVLHDWNDEECIKILKNCKEATPNNIGKVIIIEAVIDEKEESKLLDVGLM--LDMVM 333
           F+ W+LHDW DE+CIKILKNC ++ P   GK+II+E V  ++ +   L    +  +D++M
Sbjct: 285 FMKWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEFVTPKEPKGGDLSSNTVFAMDLLM 344

Query: 334 MAHTNKGKERTREEWAYVINTAGFSRYTITPIRAVQSVIQ 354
           +   + GKER+  ++  +   +GF R  I  +    SVI+
Sbjct: 345 LTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVIE 378

The following BLAST results are available for this feature:

BLAST of Sgr015218 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135713.1	5.5e-152	74.72	(R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia]	[more]
KAG6572139.1	3.1e-147	70.79	Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022136086.1	1.2e-146	73.60	3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica char...	[more]
XP_023554119.1	2.0e-146	70.22	(RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022135685.1	2.0e-146	72.75	desmethylxanthohumol 6'-O-methyltransferase-like [Momordica charantia]	[more]

BLAST of Sgr015218 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9T003	3.3e-99	50.42	Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1...	[more]
B0ZB56	6.9e-73	43.05	Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1	[more]
Q6WUC1	2.6e-72	42.94	(RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT P...	[more]
Q5C9L7	8.5e-71	39.28	(RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=...	[more]
Q6WUC2	3.2e-70	40.40	(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=...	[more]

BLAST of Sgr015218 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C5N1	2.7e-152	74.72	(R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1C2I8	5.8e-147	73.60	3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica ch...	[more]
A0A6J1C1Q8	9.8e-147	72.75	desmethylxanthohumol 6'-O-methyltransferase-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1GLX2	1.3e-146	71.07	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1C6X0	4.9e-146	73.03	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=...	[more]

BLAST of Sgr015218 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	2.3e-100	50.42	O-methyltransferase family protein	[more]
AT4G35150.1	4.4e-83	45.30	O-methyltransferase family protein	[more]
AT5G54160.1	5.8e-51	35.67	O-methyltransferase 1	[more]
AT1G77530.1	9.7e-46	36.18	O-methyltransferase family protein	[more]
AT1G77520.1	3.1e-44	35.00	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 1..356 e-value: 1.2E-118 score: 394.1
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 10..349
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 23..356 score: 74.447792
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 35..77 e-value: 9.6E-11 score: 41.5
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 4..104 e-value: 1.7E-27 score: 97.0
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 128..338 e-value: 5.5E-54 score: 182.7
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 106..356 e-value: 8.3E-79 score: 266.2
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 34..354 e-value: 2.5E-87 score: 290.5
None	No IPR available	PANTHER	PTHR11746:SF191	3'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKE	coord: 10..349
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 13..107
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 111..353

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr015218.1	Sgr015218.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity