Sgr014431 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr014431
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionAldose reductase
Locationtig00000589: 342753 .. 344458 (-)
RNA-Seq ExpressionSgr014431
SyntenySgr014431
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACAGGCTGTGATGACACCGCAGGATCAGGATGCAGAGCGTTATTTTAGGCTGTTGAGTGGGCACAGAATACCAGCAGTTGGATTAGGCACTTGGAGATCTGGTTCTCGAGCCGATGATTCTGTGTTCACTGCTATTGTTGAGGTATTGTATTCTCATGTCAAAAGTAAAGCTTTCACGTTTGCTGGTTTATAACGTTCTTGCTGATTTCATGGGACTTAACTTTGACGATAGGCTGGTTACAGACACATAGATACTGCAGCCGAATATGGACTATGGAGTTCACGAAGAGGCACTCTCTCTCTCTCTCTCTCTCGATTCTAGACGTTTCATTTTCATTATAATGACAATTAGATTGCAAAACTTTGCTCTTCTGTAAAATTTTTGTAGGTAGGTTTTGGTCTACAGGCGGCAATGAAAGCGGGGATCCGTAGGGAAGATGTTTTGATCACCACTAAGCTCTGGTAAACGAGTTTGCTCATGTAACTAGAATGAATGTTGAGTGTGAATACGACACTCAAACTTAGTTCATATCGGTTCTTGTCTGCTGGCATGTTTAGGTGCTCCGACCTATCCCCAGACAGAGTAAGAGTCGCATTAAACAATGCTCTTGAAGAACTCCAAGTTGTCTACCTTGATCTTTTCTTGGTATTTTTTTAAAAACTAATCCCATTTGGTTGATATCGCCTAAAGTTCATTTTTAATATGAAAAATTAACTACTTGTACATCAGATACACTGGCCTTTCCATCTTAAAGAAGGAGCTAGCAGGCCTCTCAATGAAGGGGAAGTTTTGGAATTGGACATGGAAGGCGTTTGGAGGGAAATGGAGAAGCTTGTGAAGGACAATCTTGTCAGGGACATCGGAATTAGCAATTTTACTTTAAAGAAATCAACAACTTACTTCTTTTTGCCCAAACAATGCTCTTGTCTTGTGTTGTGCCCAGATGGAAATGCATCCAGGATGGAGGAATGATAAAATGCTGGAGGCTTGTAAGAAAAATGGCATCCATGTCACTGTAAGACTTGCATCTTACTTGGAATTATTTGAAAACTATACTCATCAGTTTCCCCAATCATTTTAAACACTTGGCTCGAAAGTATTATGATTCTGTCACGTTTTATAAATATTTTTAACGAATAAATATCATCACGATTTCATTAACTGACAGAATTGTATAATGTTGCTAGTAAAATGGTCTAAATTGCACGAGTGTAATCTGCAATTTATTATATTTTACGCAAGGCCATTAATGAAAATAGAGTAAATTTATTGTATTGTAGGCTTATTCGCCTCTGGGCTCCTCGGAAGGAGGCAGAGATTTAATCCACGATGAGACAGTTGAAAGGATTGCAAAGAAGTTGAGCAAAAGCCCTGGCCAAATTCTTGTGAGGTGGGCTCTTCAGAGGGGAACTAGTGCCATTCCAAAATCAACCCACCCAGAAAGAATTAAAGAGAACATTACGGTGTTCGGATGGGATATTCCTGAGGACTTTCAAGCTCTTTGCAGGATCCCATATCAGGTTAAAAAAAAAAAAATCAAAATTTTTTATTGTTGCAAACTAAATAATAGAAGAATAGGAATTGAAAAGGGGATGTGATTTTGTGGCGTAGAGACGAGTGCTAAATGGAGTAGAGCTATTTGTGGCGTAGAGACGAGTGCTAAATCGAGTGTGGCTGATGTTTGGGATCACGAGGATTGA

mRNA sequence

ATGGCACAGGCTGTGATGACACCGCAGGATCAGGATGCAGAGCGTTATTTTAGGCTGTTGAGTGGGCACAGAATACCAGCAGTTGGATTAGGCACTTGGAGATCTGGTTCTCGAGCCGATGATTCTGTGTTCACTGCTATTGTTGAGGTAGGTTTTGGTCTACAGGCGGCAATGAAAGCGGGGATCCGTAGGGAAGATGTTTTGATCACCACTAAGCTCTGGTGCTCCGACCTATCCCCAGACAGAGTAAGAGTCGCATTAAACAATGCTCTTGAAGAACTCCAAGTTGTCTACCTTGATCTTTTCTTGATACACTGGCCTTTCCATCTTAAAGAAGGAGCTAGCAGGCCTCTCAATGAAGGGGAAGTTTTGGAATTGGACATGGAAGGCGTTTGGAGGGAAATGGAGAAGCTTGTGAAGGACAATCTTATGGAAATGCATCCAGGATGGAGGAATGATAAAATGCTGGAGGCTTGTAAGAAAAATGGCATCCATGTCACTGCTTATTCGCCTCTGGGCTCCTCGGAAGGAGGCAGAGATTTAATCCACGATGAGACAGTTGAAAGGATTGCAAAGAAGTTGAGCAAAAGCCCTGGCCAAATTCTTGTGAGGTGGGCTCTTCAGAGGGGAACTAGTGCCATTCCAAAATCAACCCACCCAGAAAGAATTAAAGAGAACATTACGGTGTTCGGATGGGATATTCCTGAGGACTTTCAAGCTCTTTGCAGGATCCCATATCAGACGAGTGCTAAATGGAGTAGAGCTATTTGTGGCGTAGAGACGAGTGCTAAATCGAGTGTGGCTGATGTTTGGGATCACGAGGATTGA

Coding sequence (CDS)

ATGGCACAGGCTGTGATGACACCGCAGGATCAGGATGCAGAGCGTTATTTTAGGCTGTTGAGTGGGCACAGAATACCAGCAGTTGGATTAGGCACTTGGAGATCTGGTTCTCGAGCCGATGATTCTGTGTTCACTGCTATTGTTGAGGTAGGTTTTGGTCTACAGGCGGCAATGAAAGCGGGGATCCGTAGGGAAGATGTTTTGATCACCACTAAGCTCTGGTGCTCCGACCTATCCCCAGACAGAGTAAGAGTCGCATTAAACAATGCTCTTGAAGAACTCCAAGTTGTCTACCTTGATCTTTTCTTGATACACTGGCCTTTCCATCTTAAAGAAGGAGCTAGCAGGCCTCTCAATGAAGGGGAAGTTTTGGAATTGGACATGGAAGGCGTTTGGAGGGAAATGGAGAAGCTTGTGAAGGACAATCTTATGGAAATGCATCCAGGATGGAGGAATGATAAAATGCTGGAGGCTTGTAAGAAAAATGGCATCCATGTCACTGCTTATTCGCCTCTGGGCTCCTCGGAAGGAGGCAGAGATTTAATCCACGATGAGACAGTTGAAAGGATTGCAAAGAAGTTGAGCAAAAGCCCTGGCCAAATTCTTGTGAGGTGGGCTCTTCAGAGGGGAACTAGTGCCATTCCAAAATCAACCCACCCAGAAAGAATTAAAGAGAACATTACGGTGTTCGGATGGGATATTCCTGAGGACTTTCAAGCTCTTTGCAGGATCCCATATCAGACGAGTGCTAAATGGAGTAGAGCTATTTGTGGCGTAGAGACGAGTGCTAAATCGAGTGTGGCTGATGTTTGGGATCACGAGGATTGA

Protein sequence

MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVEVGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWPFHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNLMEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKLSKSPGQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIPEDFQALCRIPYQTSAKWSRAICGVETSAKSSVADVWDHED
Homology
BLAST of Sgr014431 vs. NCBI nr
Match: KAG6596320.1 (hypothetical protein SDJN03_09500, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 451.4 bits (1160), Expect = 5.4e-123
Identity = 229/306 (74.84%), Postives = 244/306 (79.74%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVEVGFGLQAAMKA 60
           MAQA MT QD+  ERYF L+SGHRIPAVGLGTWRS SRADDSVFTAIVEVGFGLQAA+KA
Sbjct: 485 MAQAAMTVQDE--ERYFTLVSGHRIPAVGLGTWRSDSRADDSVFTAIVEVGFGLQAAIKA 544

Query: 61  GIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWPFHLKEGASRPLNE 120
           GIRRED+ ITTKLWCSDLSPDRVR+ALNNAL+ELQV YLDLFLIHWPFHLKEGASRP  E
Sbjct: 545 GIRREDLFITTKLWCSDLSPDRVRIALNNALQELQVDYLDLFLIHWPFHLKEGASRPPKE 604

Query: 121 GEVLELDMEGVWREMEKLVKDNL-----------------------------MEMHPGWR 180
           GEVLELDMEGVWREMEKLVK+NL                             MEMHPGWR
Sbjct: 605 GEVLELDMEGVWREMEKLVKENLVRDIGISNFTVKKLEKLLRFAQTMPSVCQMEMHPGWR 664

Query: 181 NDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKLSKSPGQILVRWALQRGT 240
           NDKMLEAC+KNGIHVTAYSPLGSSEGGRDLIHDE V+R+AKKL+K+PGQILVRWA+QRGT
Sbjct: 665 NDKMLEACRKNGIHVTAYSPLGSSEGGRDLIHDEAVQRVAKKLNKTPGQILVRWAIQRGT 724

Query: 241 SAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSA-KWSRAICGVETSAKSSVAD 276
           SAIPKSTH ERIKENI VFGWDIP EDF+ALCRIP Q             E     +VAD
Sbjct: 725 SAIPKSTHSERIKENIGVFGWDIPNEDFEALCRIPNQKRVLSGEDLFVNKEAGPLRTVAD 784

BLAST of Sgr014431 vs. NCBI nr
Match: XP_038904846.1 (aldose reductase isoform X1 [Benincasa hispida])

HSP 1 Score: 450.3 bits (1157), Expect = 1.2e-122
Identity = 232/323 (71.83%), Postives = 244/323 (75.54%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIV------------ 60
           MAQAVMTP+ QDAERYF L+SGHRIPAVGLGTWRSGSRADDSVFTAIV            
Sbjct: 4   MAQAVMTPEVQDAERYFTLVSGHRIPAVGLGTWRSGSRADDSVFTAIVEAGYRHIDTAAE 63

Query: 61  -----EVGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFL 120
                EVGFGLQAA+KAGIRRED+ +TTKLWCSDLSPDRVR+ LNNAL+ELQV YLDL L
Sbjct: 64  YGVHEEVGFGLQAAIKAGIRREDIFVTTKLWCSDLSPDRVRIGLNNALQELQVDYLDLLL 123

Query: 121 IHWPFHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL-------------------- 180
           IHWPFHLKEGASRP  EGEVL+LDMEGVWREMEKLVK+NL                    
Sbjct: 124 IHWPFHLKEGASRPPKEGEVLDLDMEGVWREMEKLVKENLVRDIGISNFTLKKLDNLLRF 183

Query: 181 ---------MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKL 240
                    MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSS GGRDLIHDE VERIAKKL
Sbjct: 184 AQTLPSVCQMEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSGGGRDLIHDEEVERIAKKL 243

Query: 241 SKSPGQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSA-KW 276
           +K+PGQILVRWALQRGTSAIPKSTH ERIKENI VFGW+IP EDFQALCR P Q      
Sbjct: 244 NKTPGQILVRWALQRGTSAIPKSTHSERIKENIAVFGWEIPVEDFQALCRFPNQKRVLSG 303

BLAST of Sgr014431 vs. NCBI nr
Match: TYK24925.1 (aldose reductase [Cucumis melo var. makuwa])

HSP 1 Score: 448.4 bits (1152), Expect = 4.6e-122
Identity = 229/319 (71.79%), Postives = 245/319 (76.80%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVE----------- 60
           MAQAVMTPQ QDAERYF L+SGHRIPAVGLGTWRSGSRADDSVF AIVE           
Sbjct: 1   MAQAVMTPQVQDAERYFTLVSGHRIPAVGLGTWRSGSRADDSVFNAIVEAGYRHIDTAAE 60

Query: 61  --VGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWP 120
             VGFGLQAA+KAGIRRED+ +TTKLWCSDLSP+RVR+ LNNAL+ELQV YLDL LIHWP
Sbjct: 61  YGVGFGLQAAIKAGIRREDIFVTTKLWCSDLSPNRVRIGLNNALQELQVDYLDLLLIHWP 120

Query: 121 FHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL------------------------ 180
           FHLKEGASRP  EGEVL+LDMEGVWREMEKLVK+NL                        
Sbjct: 121 FHLKEGASRPPKEGEVLDLDMEGVWREMEKLVKENLVRDIGISNFTLKKLDNLLSFAQTM 180

Query: 181 -----MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKLSKSP 240
                MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSS+GGRDLIHDE VERIAKKL+++P
Sbjct: 181 PSVCQMEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSDGGRDLIHDEAVERIAKKLNRTP 240

Query: 241 GQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSA-KWSRAI 276
           GQILV+WALQRGTSAIPKSTH ERIKENI VFGW+IP EDF+ALCRIP Q          
Sbjct: 241 GQILVKWALQRGTSAIPKSTHLERIKENIAVFGWEIPVEDFEALCRIPTQKRVLSGEELF 300

BLAST of Sgr014431 vs. NCBI nr
Match: XP_008442354.1 (PREDICTED: aldose reductase [Cucumis melo] >XP_008442356.1 PREDICTED: aldose reductase [Cucumis melo])

HSP 1 Score: 446.8 bits (1148), Expect = 1.3e-121
Identity = 229/323 (70.90%), Postives = 245/323 (75.85%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIV------------ 60
           MAQAVMTPQ QDAERYF L+SGHRIPAVGLGTWRSGSRADDSVF AIV            
Sbjct: 24  MAQAVMTPQVQDAERYFTLVSGHRIPAVGLGTWRSGSRADDSVFNAIVEAGYRHIDTAAE 83

Query: 61  -----EVGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFL 120
                EVGFGLQAA+KAGIRRED+ +TTKLWCSDLSP+RVR+ LNNAL+ELQV YLDL L
Sbjct: 84  YGVHEEVGFGLQAAIKAGIRREDIFVTTKLWCSDLSPNRVRIGLNNALQELQVDYLDLLL 143

Query: 121 IHWPFHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL-------------------- 180
           IHWPFHLKEGASRP  EGEVL+LDMEGVWREMEKLVK+NL                    
Sbjct: 144 IHWPFHLKEGASRPPKEGEVLDLDMEGVWREMEKLVKENLVRDIGISNFTLKKLDNLLSF 203

Query: 181 ---------MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKL 240
                    MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSS+GGRDLIHDE VERIAKKL
Sbjct: 204 AQTMPSVCQMEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSDGGRDLIHDEAVERIAKKL 263

Query: 241 SKSPGQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSA-KW 276
           +++PGQILV+WALQRGTSAIPKSTH ERIKENI VFGW+IP EDF+ALCRIP Q      
Sbjct: 264 NRTPGQILVKWALQRGTSAIPKSTHLERIKENIAVFGWEIPVEDFEALCRIPTQKRVLSG 323

BLAST of Sgr014431 vs. NCBI nr
Match: XP_038904847.1 (aldose reductase isoform X2 [Benincasa hispida])

HSP 1 Score: 442.2 bits (1136), Expect = 3.3e-120
Identity = 227/318 (71.38%), Postives = 239/318 (75.16%), Query Frame = 0

Query: 6   MTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIV----------------- 65
           MTP+ QDAERYF L+SGHRIPAVGLGTWRSGSRADDSVFTAIV                 
Sbjct: 1   MTPEVQDAERYFTLVSGHRIPAVGLGTWRSGSRADDSVFTAIVEAGYRHIDTAAEYGVHE 60

Query: 66  EVGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWPF 125
           EVGFGLQAA+KAGIRRED+ +TTKLWCSDLSPDRVR+ LNNAL+ELQV YLDL LIHWPF
Sbjct: 61  EVGFGLQAAIKAGIRREDIFVTTKLWCSDLSPDRVRIGLNNALQELQVDYLDLLLIHWPF 120

Query: 126 HLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL------------------------- 185
           HLKEGASRP  EGEVL+LDMEGVWREMEKLVK+NL                         
Sbjct: 121 HLKEGASRPPKEGEVLDLDMEGVWREMEKLVKENLVRDIGISNFTLKKLDNLLRFAQTLP 180

Query: 186 ----MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKLSKSPG 245
               MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSS GGRDLIHDE VERIAKKL+K+PG
Sbjct: 181 SVCQMEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSGGGRDLIHDEEVERIAKKLNKTPG 240

Query: 246 QILVRWALQRGTSAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSA-KWSRAIC 276
           QILVRWALQRGTSAIPKSTH ERIKENI VFGW+IP EDFQALCR P Q           
Sbjct: 241 QILVRWALQRGTSAIPKSTHSERIKENIAVFGWEIPVEDFQALCRFPNQKRVLSGEELFV 300

BLAST of Sgr014431 vs. ExPASy Swiss-Prot
Match: P23901 (Aldose reductase OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 4.2e-86
Identity = 169/327 (51.68%), Postives = 211/327 (64.53%), Query Frame = 0

Query: 2   AQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVEVGF--------- 61
           A+A M   +QD   +F L SGH +PAVGLGTWR+GS    SV TAI E G+         
Sbjct: 4   AKATMGQGEQD---HFVLKSGHAMPAVGLGTWRAGSDTAHSVRTAITEAGYRHVDTAAEY 63

Query: 62  --------GLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLI 121
                   GL+AAM+AGI R+D+ +T+K+WC++L+P+RVR AL N L++LQ+ Y+DL+ I
Sbjct: 64  GVEKEVGKGLKAAMEAGIDRKDLFVTSKIWCTNLAPERVRPALENTLKDLQLDYIDLYHI 123

Query: 122 HWPFHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL--------------------- 181
           HWPF LK+GA  P   GEVLE DMEGVW+EME LVKD L                     
Sbjct: 124 HWPFRLKDGAHMPPEAGEVLEFDMEGVWKEMENLVKDGLVKDIGVCNYTVTKLNRLLRSA 183

Query: 182 --------MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKLS 241
                   MEMHPGW+NDK+ EACKK+GIHVTAYSPLGSSE  ++L HD  VE++A KL+
Sbjct: 184 KIPPAVCQMEMHPGWKNDKIFEACKKHGIHVTAYSPLGSSE--KNLAHDPVVEKVANKLN 243

Query: 242 KSPGQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSAKWSR 276
           K+PGQ+L++WALQRGTS IPKS+  ERIKENI VFGW+IP EDF+ LC I  +      R
Sbjct: 244 KTPGQVLIKWALQRGTSVIPKSSKDERIKENIQVFGWEIPEEDFKVLCSIKDE-----KR 303

BLAST of Sgr014431 vs. ExPASy Swiss-Prot
Match: Q84TF0 (Aldo-keto reductase family 4 member C10 OS=Arabidopsis thaliana OX=3702 GN=AKR4C10 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 7.3e-46
Identity = 108/313 (34.50%), Postives = 163/313 (52.08%), Query Frame = 0

Query: 15  RYFRLLSGHRIPAVGLGTWRS-----GSRADDSV------------FTAIVEVGFGLQAA 74
           R+F L +G +IP+VGLGTW++     G+  D +V            +    E+G  L+  
Sbjct: 6   RFFELNTGAKIPSVGLGTWQADPGLVGNAVDAAVKIGYRHIDCAQIYGNEKEIGLVLKKL 65

Query: 75  MKAG-IRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWPFHLKEGAS- 134
              G ++RE++ IT+KLWC+   P  V  ALN  L++LQ+ Y+DL+LIHWP  LK+G++ 
Sbjct: 66  FDGGVVKREEMFITSKLWCTYHDPQEVPEALNRTLQDLQLDYVDLYLIHWPVSLKKGSTG 125

Query: 135 -RPLNEGEVLELDMEGVWREMEKLVKD-----------------------------NLME 194
            +P N   +L  D+   W+ ME L                                N +E
Sbjct: 126 FKPEN---ILPTDIPSTWKAMESLFDSGKARAIGVSNFSSKKLADLLVVARVPPAVNQVE 185

Query: 195 MHPGWRNDKMLEACKKNGIHVTAYSPLGSSEG---GRDLIHDETVERIAKKLSKSPGQIL 254
            HP W+ + + + CK  G+H++ YSPLGS        D++ +  +  +A+KL K+P Q+ 
Sbjct: 186 CHPSWQQNVLRDFCKSKGVHLSGYSPLGSPGTTWLTSDVLKNPILGGVAEKLGKTPAQVA 245

Query: 255 VRWALQRGTSAIPKSTHPERIKENITVFGWDIPEDFQALCRIPYQTSAKWSRAICGV-ET 275
           +RW LQ G S +PKSTH +RIK+N  VF W IPED   L +       +  R +  V ET
Sbjct: 246 LRWGLQMGQSVLPKSTHEDRIKQNFDVFNWSIPEDM--LSKFSEIGQGRLVRGMSFVHET 305

BLAST of Sgr014431 vs. ExPASy Swiss-Prot
Match: Q0PGJ6 (NADPH-dependent aldo-keto reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AKR4C9 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.1e-45
Identity = 105/313 (33.55%), Postives = 155/313 (49.52%), Query Frame = 0

Query: 12  DAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVEVGF-------------GLQAAM 71
           +A  +F+L +G + P+VGLGTW++          A V++G+              + A +
Sbjct: 3   NAITFFKLNTGAKFPSVGLGTWQASPGLVGDAVAAAVKIGYRHIDCAQIYGNEKEIGAVL 62

Query: 72  K-----AGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWPFHLKEG 131
           K       ++RED+ IT+KLWC+D  P  V  ALN  L++LQ+ Y+DL+LIHWP  +K+G
Sbjct: 63  KKLFEDRVVKREDLFITSKLWCTDHDPQDVPEALNRTLKDLQLEYVDLYLIHWPARIKKG 122

Query: 132 ASRPLNEGEVLELDMEGVWREMEKLVKD-----------------------------NLM 191
            S  +    +L +D+   W+ ME L                                N +
Sbjct: 123 -SVGIKPENLLPVDIPSTWKAMEALYDSGKARAIGVSNFSTKKLADLLELARVPPAVNQV 182

Query: 192 EMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEG---GRDLIHDETVERIAKKLSKSPGQI 251
           E HP WR  K+ E CK  G+H++AYSPLGS        D++ +  +  +A+KL KSP Q+
Sbjct: 183 ECHPSWRQTKLQEFCKSKGVHLSAYSPLGSPGTTWLKSDVLKNPILNMVAEKLGKSPAQV 242

Query: 252 LVRWALQRGTSAIPKSTHPERIKENITVFGWDIPEDFQALCRIPYQTSAKWSRAICGVET 275
            +RW LQ G S +PKST+  RIKEN  VF W IP+   A      Q        +     
Sbjct: 243 ALRWGLQMGHSVLPKSTNEGRIKENFNVFDWSIPDYMFAKFAEIEQARLVTGSFLVHETL 302

BLAST of Sgr014431 vs. ExPASy Swiss-Prot
Match: Q9M338 (Aldo-keto reductase family 4 member C11 OS=Arabidopsis thaliana OX=3702 GN=AKR4C11 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 8.9e-44
Identity = 103/312 (33.01%), Postives = 158/312 (50.64%), Query Frame = 0

Query: 16  YFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVEVGF-GLQAAMKAG------------- 75
           +F+L +G +IP+VGLGTW++          A V++G+  +  A + G             
Sbjct: 7   FFQLNTGAKIPSVGLGTWQAAPGVVGDAVAAAVKIGYQHIDCASRYGNEIEIGKVLKKLF 66

Query: 76  ----IRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWPFHLKEGA--S 135
               ++RE + IT+K+W +DL P  V+ ALN  L++LQ+ Y+DL+L+HWP  LK+G    
Sbjct: 67  DDGVVKREKLFITSKIWLTDLDPPDVQDALNRTLQDLQLDYVDLYLMHWPVRLKKGTVDF 126

Query: 136 RPLNEGEVLELDMEGVWREMEKLVKD-----------------------------NLMEM 195
           +P N   ++ +D+   W+ ME LV                               N +E 
Sbjct: 127 KPEN---IMPIDIPSTWKAMEALVDSGKARAIGVSNFSTKKLSDLVEAARVPPAVNQVEC 186

Query: 196 HPGWRNDKMLEACKKNGIHVTAYSPLGS---SEGGRDLIHDETVERIAKKLSKSPGQILV 255
           HP W+  K+ E CK  GIH++ YSPLGS   +    D++    +E IAK++ KSP Q  +
Sbjct: 187 HPSWQQHKLHEFCKSKGIHLSGYSPLGSPGTTWVKADVLKSPVIEMIAKEIGKSPAQTAL 246

Query: 256 RWALQRGTSAIPKSTHPERIKENITVFGWDIPED-FQALCRIPYQTSAKWSRAICGVETS 275
           RW LQ G S +PKST+  RI+EN  V GW IP++ F    +I  Q       +      S
Sbjct: 247 RWGLQMGHSILPKSTNEGRIRENFDVLGWSIPKEMFDKFSKIE-QARLVQGTSFVHETLS 306

BLAST of Sgr014431 vs. ExPASy Swiss-Prot
Match: O80944 (Aldo-keto reductase family 4 member C8 OS=Arabidopsis thaliana OX=3702 GN=AKR4C8 PE=1 SV=2)

HSP 1 Score: 165.6 bits (418), Expect = 7.8e-40
Identity = 104/309 (33.66%), Postives = 157/309 (50.81%), Query Frame = 0

Query: 15  RYFRLLSGHRIPAVGLGTW-----------RSGSRADD--SVFTAIVEVGFGLQAAMKAG 74
           R+F L +G ++P VGLGT+           + G R  D  S++    E+G  L+  +  G
Sbjct: 6   RFFELNTGAKLPCVGLGTYAMVATAIEQAIKIGYRHIDCASIYGNEKEIGGVLKKLIGDG 65

Query: 75  -IRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWPFHLKEGASRPLNE 134
            ++RE++ IT+KLW +D  P+ V  AL   L++LQ+ Y+DL+LIHWP  LK+ +  P  E
Sbjct: 66  FVKREELFITSKLWSNDHLPEDVPKALEKTLQDLQIDYVDLYLIHWPASLKKESLMPTPE 125

Query: 135 GEVLELDMEGVWREMEKLVKD-----------------------------NLMEMHPGWR 194
             + + D+   W+ ME L                                N +E HP W+
Sbjct: 126 -MLTKPDITSTWKAMEALYDSGKARAIGVSNFSSKKLTDLLNVARVTPAVNQVECHPVWQ 185

Query: 195 NDKMLEACKKNGIHVTAYSPLGSSEGGR---DLIHDETVERIAKKLSKSPGQILVRWALQ 254
              + E CK  G+H++ YSPLGS   G     ++ +  V  +A+KL K+  Q+ +RW LQ
Sbjct: 186 QQGLHELCKSKGVHLSGYSPLGSQSKGEVRLKVLQNPIVTEVAEKLGKTTAQVALRWGLQ 245

Query: 255 RGTSAIPKSTHPERIKENITVFGWDIPED-FQALCRIPYQTSAKWSRAI-CGVETSA-KS 275
            G S +PKS+   R+KEN+ VF W IPED F     IP +   K+ RA     ET     
Sbjct: 246 TGHSVLPKSSSGARLKENLDVFDWSIPEDLFTKFSNIPQE---KFCRATEFAHETHGFYK 305

BLAST of Sgr014431 vs. ExPASy TrEMBL
Match: A0A5D3DMS9 (Aldose reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00400 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.2e-122
Identity = 229/319 (71.79%), Postives = 245/319 (76.80%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVE----------- 60
           MAQAVMTPQ QDAERYF L+SGHRIPAVGLGTWRSGSRADDSVF AIVE           
Sbjct: 1   MAQAVMTPQVQDAERYFTLVSGHRIPAVGLGTWRSGSRADDSVFNAIVEAGYRHIDTAAE 60

Query: 61  --VGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWP 120
             VGFGLQAA+KAGIRRED+ +TTKLWCSDLSP+RVR+ LNNAL+ELQV YLDL LIHWP
Sbjct: 61  YGVGFGLQAAIKAGIRREDIFVTTKLWCSDLSPNRVRIGLNNALQELQVDYLDLLLIHWP 120

Query: 121 FHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL------------------------ 180
           FHLKEGASRP  EGEVL+LDMEGVWREMEKLVK+NL                        
Sbjct: 121 FHLKEGASRPPKEGEVLDLDMEGVWREMEKLVKENLVRDIGISNFTLKKLDNLLSFAQTM 180

Query: 181 -----MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKLSKSP 240
                MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSS+GGRDLIHDE VERIAKKL+++P
Sbjct: 181 PSVCQMEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSDGGRDLIHDEAVERIAKKLNRTP 240

Query: 241 GQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSA-KWSRAI 276
           GQILV+WALQRGTSAIPKSTH ERIKENI VFGW+IP EDF+ALCRIP Q          
Sbjct: 241 GQILVKWALQRGTSAIPKSTHLERIKENIAVFGWEIPVEDFEALCRIPTQKRVLSGEELF 300

BLAST of Sgr014431 vs. ExPASy TrEMBL
Match: A0A1S3B5H9 (aldose reductase OS=Cucumis melo OX=3656 GN=LOC103486249 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 6.5e-122
Identity = 229/323 (70.90%), Postives = 245/323 (75.85%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIV------------ 60
           MAQAVMTPQ QDAERYF L+SGHRIPAVGLGTWRSGSRADDSVF AIV            
Sbjct: 24  MAQAVMTPQVQDAERYFTLVSGHRIPAVGLGTWRSGSRADDSVFNAIVEAGYRHIDTAAE 83

Query: 61  -----EVGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFL 120
                EVGFGLQAA+KAGIRRED+ +TTKLWCSDLSP+RVR+ LNNAL+ELQV YLDL L
Sbjct: 84  YGVHEEVGFGLQAAIKAGIRREDIFVTTKLWCSDLSPNRVRIGLNNALQELQVDYLDLLL 143

Query: 121 IHWPFHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL-------------------- 180
           IHWPFHLKEGASRP  EGEVL+LDMEGVWREMEKLVK+NL                    
Sbjct: 144 IHWPFHLKEGASRPPKEGEVLDLDMEGVWREMEKLVKENLVRDIGISNFTLKKLDNLLSF 203

Query: 181 ---------MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKL 240
                    MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSS+GGRDLIHDE VERIAKKL
Sbjct: 204 AQTMPSVCQMEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSDGGRDLIHDEAVERIAKKL 263

Query: 241 SKSPGQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSA-KW 276
           +++PGQILV+WALQRGTSAIPKSTH ERIKENI VFGW+IP EDF+ALCRIP Q      
Sbjct: 264 NRTPGQILVKWALQRGTSAIPKSTHLERIKENIAVFGWEIPVEDFEALCRIPTQKRVLSG 323

BLAST of Sgr014431 vs. ExPASy TrEMBL
Match: A0A0A0LDB1 (Aldo_ket_red domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G730760 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 7.9e-120
Identity = 226/323 (69.97%), Postives = 244/323 (75.54%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIV------------ 60
           MAQAV+T Q QDAERYF L+SGHRIPAVGLGTWRSGSRA++SVF A+V            
Sbjct: 1   MAQAVVTSQVQDAERYFTLVSGHRIPAVGLGTWRSGSRANESVFNALVEAGYRHIDTAAE 60

Query: 61  -----EVGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFL 120
                EVGFGLQAA+K GIRRED+ +TTKLWCSDLSP+RVR+ LNNAL+ELQV YLDL L
Sbjct: 61  YGVHEEVGFGLQAAIKTGIRREDIFVTTKLWCSDLSPNRVRIGLNNALQELQVDYLDLLL 120

Query: 121 IHWPFHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL-------------------- 180
           IHWPFHLKEGASRP  EGEVL+LDMEGVWREMEKLVK+NL                    
Sbjct: 121 IHWPFHLKEGASRPPKEGEVLDLDMEGVWREMEKLVKENLVRDIGISNFTLKKLDNLLSF 180

Query: 181 ---------MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKL 240
                    MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIH+ETVERIAKKL
Sbjct: 181 AQTMPSVCQMEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHNETVERIAKKL 240

Query: 241 SKSPGQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSA-KW 276
           +K+PGQILV+WALQRGTSAIPKSTH ERIKENI VFGW+IP EDFQALCRIP Q      
Sbjct: 241 NKTPGQILVKWALQRGTSAIPKSTHKERIKENIGVFGWEIPVEDFQALCRIPTQKRVLSG 300

BLAST of Sgr014431 vs. ExPASy TrEMBL
Match: A0A6J1G6J8 (aldose reductase OS=Cucurbita moschata OX=3662 GN=LOC111451333 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 7.9e-120
Identity = 228/323 (70.59%), Postives = 244/323 (75.54%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVE----------- 60
           MAQA MT QD+  ERYF L+SGHRIPAVGLGTWRSGSR+DDSVFTAIVE           
Sbjct: 1   MAQAAMTVQDE--ERYFTLVSGHRIPAVGLGTWRSGSRSDDSVFTAIVEAGYRHIDTAAE 60

Query: 61  ------VGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFL 120
                 VGFGLQAA+KAGIRRED+ ITTKLWCSDLSPDRVR+ALNNAL+ELQV YLDL L
Sbjct: 61  YGVHEQVGFGLQAAIKAGIRREDLFITTKLWCSDLSPDRVRIALNNALQELQVDYLDLLL 120

Query: 121 IHWPFHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL-------------------- 180
           IHWPFHLKEGASRP  EGEVLELDMEGVWREMEKLVK+NL                    
Sbjct: 121 IHWPFHLKEGASRPPKEGEVLELDMEGVWREMEKLVKENLVRDIGISNFTVKKLEKLLRF 180

Query: 181 ---------MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKL 240
                    MEMHPGWRNDKMLEAC+KNGIHVTAYSPLGSSEGGRDLIHDE V+R+AKKL
Sbjct: 181 AQTMPSVCQMEMHPGWRNDKMLEACRKNGIHVTAYSPLGSSEGGRDLIHDEAVQRVAKKL 240

Query: 241 SKSPGQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSA-KW 276
           +K+PGQILVRWA+QRGTSAIPKSTH ERIKENI VFGWDIP EDF+ALCRIP Q      
Sbjct: 241 NKTPGQILVRWAIQRGTSAIPKSTHSERIKENIGVFGWDIPNEDFEALCRIPNQKRVLSG 300

BLAST of Sgr014431 vs. ExPASy TrEMBL
Match: A0A6J1I904 (aldose reductase OS=Cucurbita maxima OX=3661 GN=LOC111470262 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 3.0e-119
Identity = 228/323 (70.59%), Postives = 241/323 (74.61%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVE----------- 60
           MAQA MT QD+  E YF L+SGHRIPAVGLGTWRSGS  DDSVFTAIVE           
Sbjct: 1   MAQAAMTVQDE--EHYFTLVSGHRIPAVGLGTWRSGSWPDDSVFTAIVEAGYRHIDTAAE 60

Query: 61  ------VGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFL 120
                 VGFGLQAAMKAGIRRED+ ITTKLWCSDLSPDRVR+ALNNAL+ELQV YLDLFL
Sbjct: 61  YGVHEQVGFGLQAAMKAGIRREDLFITTKLWCSDLSPDRVRIALNNALQELQVDYLDLFL 120

Query: 121 IHWPFHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL-------------------- 180
           IHWPFHLKEGASRP  EGEVLELDMEGVWREMEKLVK+NL                    
Sbjct: 121 IHWPFHLKEGASRPPKEGEVLELDMEGVWREMEKLVKENLVRDIGISNFTVKKLDNLLRF 180

Query: 181 ---------MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKL 240
                    MEMHPGWRNDKMLEAC+KNGIHVTAYSPLGSSEGGRDLIHDE +ER+AKKL
Sbjct: 181 AQTMPSVCQMEMHPGWRNDKMLEACRKNGIHVTAYSPLGSSEGGRDLIHDEAIERVAKKL 240

Query: 241 SKSPGQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIP-EDFQALCRIPYQTSA-KW 276
            K+PGQILVRWA+QRGTSAIPKSTH ERIKENI VFGW+IP EDF+ALCRIP Q      
Sbjct: 241 KKTPGQILVRWAIQRGTSAIPKSTHSERIKENIGVFGWEIPNEDFEALCRIPNQKRVLSG 300

BLAST of Sgr014431 vs. TAIR 10
Match: AT5G01670.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 349.0 bits (894), Expect = 3.5e-96
Identity = 187/328 (57.01%), Postives = 219/328 (66.77%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIV------------ 60
           MA A  T + Q+ E  FRLLSGH+IPAVGLGTWRSGS+A  +V TAIV            
Sbjct: 1   MAHATFTSEGQNMES-FRLLSGHKIPAVGLGTWRSGSQAAHAVVTAIVEGGYRHIDTAWE 60

Query: 61  -----EVGFGLQAAMKAGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFL 120
                EVG G++ AM AG+ R D+ +T+KLWC++LSP+RVR AL N L+ELQ+ YLDL+L
Sbjct: 61  YGDQREVGQGIKRAMHAGLERRDLFVTSKLWCTELSPERVRPALQNTLKELQLEYLDLYL 120

Query: 121 IHWPFHLKEGASRPLNEGEVLELDMEGVWREMEKLVKDNL-------------------- 180
           IHWP  L+EGAS+P   G+VL+ DMEGVWREME L KD+L                    
Sbjct: 121 IHWPIRLREGASKPPKAGDVLDFDMEGVWREMENLSKDSLVRNIGVCNFTVTKLNKLLGF 180

Query: 181 ---------MEMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEGGRDLIHDETVERIAKKL 240
                    MEMHPGWRND++LE CKKN IHVTAYSPLGS EGGRDLIHD+TV+RIAKKL
Sbjct: 181 AELIPAVCQMEMHPGWRNDRILEFCKKNEIHVTAYSPLGSQEGGRDLIHDQTVDRIAKKL 240

Query: 241 SKSPGQILVRWALQRGTSAIPKSTHPERIKENITVFGWDIPE-DFQALCRIPYQTSAKWS 276
           +K+PGQILV+W LQRGTS IPKS +PERIKENI VF W IPE DFQAL  I  Q      
Sbjct: 241 NKTPGQILVKWGLQRGTSVIPKSLNPERIKENIKVFDWVIPEQDFQALNSITDQ-----K 300

BLAST of Sgr014431 vs. TAIR 10
Match: AT5G01670.2 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 334.3 bits (856), Expect = 9.0e-92
Identity = 187/355 (52.68%), Postives = 219/355 (61.69%), Query Frame = 0

Query: 1   MAQAVMTPQDQDAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIV------------ 60
           MA A  T + Q+ E  FRLLSGH+IPAVGLGTWRSGS+A  +V TAIV            
Sbjct: 1   MAHATFTSEGQNMES-FRLLSGHKIPAVGLGTWRSGSQAAHAVVTAIVEGGYRHIDTAWE 60

Query: 61  -----EVGFGLQAAMKAGIRREDVLITTKLW---------------------------CS 120
                EVG G++ AM AG+ R D+ +T+KLW                           C+
Sbjct: 61  YGDQREVGQGIKRAMHAGLERRDLFVTSKLWYTLILRKMINLSSPLMNVLVGTCLNKRCT 120

Query: 121 DLSPDRVRVALNNALEELQVVYLDLFLIHWPFHLKEGASRPLNEGEVLELDMEGVWREME 180
           +LSP+RVR AL N L+ELQ+ YLDL+LIHWP  L+EGAS+P   G+VL+ DMEGVWREME
Sbjct: 121 ELSPERVRPALQNTLKELQLEYLDLYLIHWPIRLREGASKPPKAGDVLDFDMEGVWREME 180

Query: 181 KLVKDNL-----------------------------MEMHPGWRNDKMLEACKKNGIHVT 240
            L KD+L                             MEMHPGWRND++LE CKKN IHVT
Sbjct: 181 NLSKDSLVRNIGVCNFTVTKLNKLLGFAELIPAVCQMEMHPGWRNDRILEFCKKNEIHVT 240

Query: 241 AYSPLGSSEGGRDLIHDETVERIAKKLSKSPGQILVRWALQRGTSAIPKSTHPERIKENI 276
           AYSPLGS EGGRDLIHD+TV+RIAKKL+K+PGQILV+W LQRGTS IPKS +PERIKENI
Sbjct: 241 AYSPLGSQEGGRDLIHDQTVDRIAKKLNKTPGQILVKWGLQRGTSVIPKSLNPERIKENI 300

BLAST of Sgr014431 vs. TAIR 10
Match: AT2G37790.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 5.2e-47
Identity = 108/313 (34.50%), Postives = 163/313 (52.08%), Query Frame = 0

Query: 15  RYFRLLSGHRIPAVGLGTWRS-----GSRADDSV------------FTAIVEVGFGLQAA 74
           R+F L +G +IP+VGLGTW++     G+  D +V            +    E+G  L+  
Sbjct: 6   RFFELNTGAKIPSVGLGTWQADPGLVGNAVDAAVKIGYRHIDCAQIYGNEKEIGLVLKKL 65

Query: 75  MKAG-IRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWPFHLKEGAS- 134
              G ++RE++ IT+KLWC+   P  V  ALN  L++LQ+ Y+DL+LIHWP  LK+G++ 
Sbjct: 66  FDGGVVKREEMFITSKLWCTYHDPQEVPEALNRTLQDLQLDYVDLYLIHWPVSLKKGSTG 125

Query: 135 -RPLNEGEVLELDMEGVWREMEKLVKD-----------------------------NLME 194
            +P N   +L  D+   W+ ME L                                N +E
Sbjct: 126 FKPEN---ILPTDIPSTWKAMESLFDSGKARAIGVSNFSSKKLADLLVVARVPPAVNQVE 185

Query: 195 MHPGWRNDKMLEACKKNGIHVTAYSPLGSSEG---GRDLIHDETVERIAKKLSKSPGQIL 254
            HP W+ + + + CK  G+H++ YSPLGS        D++ +  +  +A+KL K+P Q+ 
Sbjct: 186 CHPSWQQNVLRDFCKSKGVHLSGYSPLGSPGTTWLTSDVLKNPILGGVAEKLGKTPAQVA 245

Query: 255 VRWALQRGTSAIPKSTHPERIKENITVFGWDIPEDFQALCRIPYQTSAKWSRAICGV-ET 275
           +RW LQ G S +PKSTH +RIK+N  VF W IPED   L +       +  R +  V ET
Sbjct: 246 LRWGLQMGQSVLPKSTHEDRIKQNFDVFNWSIPEDM--LSKFSEIGQGRLVRGMSFVHET 305

BLAST of Sgr014431 vs. TAIR 10
Match: AT2G37770.2 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.5e-46
Identity = 105/313 (33.55%), Postives = 155/313 (49.52%), Query Frame = 0

Query: 12  DAERYFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVEVGF-------------GLQAAM 71
           +A  +F+L +G + P+VGLGTW++          A V++G+              + A +
Sbjct: 3   NAITFFKLNTGAKFPSVGLGTWQASPGLVGDAVAAAVKIGYRHIDCAQIYGNEKEIGAVL 62

Query: 72  K-----AGIRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWPFHLKEG 131
           K       ++RED+ IT+KLWC+D  P  V  ALN  L++LQ+ Y+DL+LIHWP  +K+G
Sbjct: 63  KKLFEDRVVKREDLFITSKLWCTDHDPQDVPEALNRTLKDLQLEYVDLYLIHWPARIKKG 122

Query: 132 ASRPLNEGEVLELDMEGVWREMEKLVKD-----------------------------NLM 191
            S  +    +L +D+   W+ ME L                                N +
Sbjct: 123 -SVGIKPENLLPVDIPSTWKAMEALYDSGKARAIGVSNFSTKKLADLLELARVPPAVNQV 182

Query: 192 EMHPGWRNDKMLEACKKNGIHVTAYSPLGSSEG---GRDLIHDETVERIAKKLSKSPGQI 251
           E HP WR  K+ E CK  G+H++AYSPLGS        D++ +  +  +A+KL KSP Q+
Sbjct: 183 ECHPSWRQTKLQEFCKSKGVHLSAYSPLGSPGTTWLKSDVLKNPILNMVAEKLGKSPAQV 242

Query: 252 LVRWALQRGTSAIPKSTHPERIKENITVFGWDIPEDFQALCRIPYQTSAKWSRAICGVET 275
            +RW LQ G S +PKST+  RIKEN  VF W IP+   A      Q        +     
Sbjct: 243 ALRWGLQMGHSVLPKSTNEGRIKENFNVFDWSIPDYMFAKFAEIEQARLVTGSFLVHETL 302

BLAST of Sgr014431 vs. TAIR 10
Match: AT3G53880.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 6.3e-45
Identity = 103/312 (33.01%), Postives = 158/312 (50.64%), Query Frame = 0

Query: 16  YFRLLSGHRIPAVGLGTWRSGSRADDSVFTAIVEVGF-GLQAAMKAG------------- 75
           +F+L +G +IP+VGLGTW++          A V++G+  +  A + G             
Sbjct: 7   FFQLNTGAKIPSVGLGTWQAAPGVVGDAVAAAVKIGYQHIDCASRYGNEIEIGKVLKKLF 66

Query: 76  ----IRREDVLITTKLWCSDLSPDRVRVALNNALEELQVVYLDLFLIHWPFHLKEGA--S 135
               ++RE + IT+K+W +DL P  V+ ALN  L++LQ+ Y+DL+L+HWP  LK+G    
Sbjct: 67  DDGVVKREKLFITSKIWLTDLDPPDVQDALNRTLQDLQLDYVDLYLMHWPVRLKKGTVDF 126

Query: 136 RPLNEGEVLELDMEGVWREMEKLVKD-----------------------------NLMEM 195
           +P N   ++ +D+   W+ ME LV                               N +E 
Sbjct: 127 KPEN---IMPIDIPSTWKAMEALVDSGKARAIGVSNFSTKKLSDLVEAARVPPAVNQVEC 186

Query: 196 HPGWRNDKMLEACKKNGIHVTAYSPLGS---SEGGRDLIHDETVERIAKKLSKSPGQILV 255
           HP W+  K+ E CK  GIH++ YSPLGS   +    D++    +E IAK++ KSP Q  +
Sbjct: 187 HPSWQQHKLHEFCKSKGIHLSGYSPLGSPGTTWVKADVLKSPVIEMIAKEIGKSPAQTAL 246

Query: 256 RWALQRGTSAIPKSTHPERIKENITVFGWDIPED-FQALCRIPYQTSAKWSRAICGVETS 275
           RW LQ G S +PKST+  RI+EN  V GW IP++ F    +I  Q       +      S
Sbjct: 247 RWGLQMGHSILPKSTNEGRIRENFDVLGWSIPKEMFDKFSKIE-QARLVQGTSFVHETLS 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6596320.15.4e-12374.84hypothetical protein SDJN03_09500, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_038904846.11.2e-12271.83aldose reductase isoform X1 [Benincasa hispida][more]
TYK24925.14.6e-12271.79aldose reductase [Cucumis melo var. makuwa][more]
XP_008442354.11.3e-12170.90PREDICTED: aldose reductase [Cucumis melo] >XP_008442356.1 PREDICTED: aldose red... [more]
XP_038904847.13.3e-12071.38aldose reductase isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P239014.2e-8651.68Aldose reductase OS=Hordeum vulgare OX=4513 PE=1 SV=1[more]
Q84TF07.3e-4634.50Aldo-keto reductase family 4 member C10 OS=Arabidopsis thaliana OX=3702 GN=AKR4C... [more]
Q0PGJ62.1e-4533.55NADPH-dependent aldo-keto reductase, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
Q9M3388.9e-4433.01Aldo-keto reductase family 4 member C11 OS=Arabidopsis thaliana OX=3702 GN=AKR4C... [more]
O809447.8e-4033.66Aldo-keto reductase family 4 member C8 OS=Arabidopsis thaliana OX=3702 GN=AKR4C8... [more]
Match NameE-valueIdentityDescription
A0A5D3DMS92.2e-12271.79Aldose reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004... [more]
A0A1S3B5H96.5e-12270.90aldose reductase OS=Cucumis melo OX=3656 GN=LOC103486249 PE=4 SV=1[more]
A0A0A0LDB17.9e-12069.97Aldo_ket_red domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G73076... [more]
A0A6J1G6J87.9e-12070.59aldose reductase OS=Cucurbita moschata OX=3662 GN=LOC111451333 PE=3 SV=1[more]
A0A6J1I9043.0e-11970.59aldose reductase OS=Cucurbita maxima OX=3661 GN=LOC111470262 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G01670.13.5e-9657.01NAD(P)-linked oxidoreductase superfamily protein [more]
AT5G01670.29.0e-9252.68NAD(P)-linked oxidoreductase superfamily protein [more]
AT2G37790.15.2e-4734.50NAD(P)-linked oxidoreductase superfamily protein [more]
AT2G37770.21.5e-4633.55NAD(P)-linked oxidoreductase superfamily protein [more]
AT3G53880.16.3e-4533.01NAD(P)-linked oxidoreductase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020471Aldo-keto reductasePRINTSPR00069ALDKETRDTASEcoord: 181..205
score: 35.11
coord: 89..107
score: 54.24
IPR020471Aldo-keto reductasePIRSFPIRSF000097AKRcoord: 141..255
e-value: 6.2E-26
score: 88.9
coord: 10..52
e-value: 7.5E-4
score: 16.3
coord: 42..144
e-value: 3.4E-23
score: 79.9
IPR023210NADP-dependent oxidoreductase domainPFAMPF00248Aldo_ket_redcoord: 57..140
e-value: 3.2E-11
score: 42.9
coord: 153..238
e-value: 1.2E-9
score: 37.8
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3D3.20.20.100coord: 4..142
e-value: 4.9E-35
score: 122.8
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3D3.20.20.100coord: 143..275
e-value: 1.3E-33
score: 118.1
IPR036812NADP-dependent oxidoreductase domain superfamilySUPERFAMILY51430NAD(P)-linked oxidoreductasecoord: 19..240
NoneNo IPR availablePANTHERPTHR11732:SF164NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEINcoord: 14..143
NoneNo IPR availablePANTHERPTHR11732:SF164NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEINcoord: 144..274
NoneNo IPR availablePANTHERPTHR11732ALDO/KETO REDUCTASEcoord: 14..143
NoneNo IPR availablePANTHERPTHR11732ALDO/KETO REDUCTASEcoord: 144..274
IPR018170Aldo/keto reductase, conserved sitePROSITEPS00063ALDOKETO_REDUCTASE_3coord: 214..229

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr014431.1Sgr014431.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
molecular_function GO:0004032 alditol:NADP+ 1-oxidoreductase activity
molecular_function GO:0047834 D-threo-aldose 1-dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity