Sgr011884 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr011884
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	cytochrome b5
Location	tig00153113: 84912 .. 86693 (+)
RNA-Seq Expression	Sgr011884
Synteny	Sgr011884

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCGAACGATAAAGTGTATACTTTCGATGAAGTTGCGAAGCACAACCACCAGAAGGATTGTTGGCTTATAATATCTGGAAATGTATGCTCTCTCTTTCTCTTTCTTTTTTTCTATGAGGGTGTTTCAAAATGCTGATGAGTTAGAGAAATCTAATCCATCTAGCTTAAATCGAGTGAGTTCGGTTTGATTTTGGATTGTAAATTTGTTTGAGTTGGATTAAGTTCAAATAAACGAAACTTTTACGAGTTGAGTTTGGTTTATGGATTTTTATCTCAAATTAGTTTAGGGGTTAACATAAGGCGGATCTAAGAAGTGGAGTAATTGCCGACATAAGATTTTAATGTTTTTCTTGTTCTTTTTTGGTATATGGGTTATATTCGTGCAGGTAGAGATTCAAACTTTTGACCGATGAAAAAGTTACTTATACCTTAAGTTATTGTTTGTTAGATTTTTGCTCCTCTTTAAGAAAATTTCTGATTGGAGGGAAGGTTACCAATGCTTTCGACTTATAAGAAAGGTTATCGATACTTTCGACTTACAAGAGAAGGTTAATGTGATGCCTTAGTTGAGCTATTCACATGTTAAATTAATGTTTTCCTGCTAATTTGTTCTAGGATTGAAAAATTTTATATTTGGGAACTTGGAAAGTCAAATCTAGCCCCCTTTAAAAAATTTCTGGCTCCTGGCTAATTGAAGCAATGAAAGTTAATTGTTAATGAAAAGAACTTTTCTTTTACTTATGATAATATATATTTTAATTTTCCTTTTCCTAACTCATATTGGTTGCATTTGTCAAATTCGAAACGTATATAAAATAATTGGGTTGGATCTAAAATAATACGTTGATCAAACCCGACTTATTAATTATTTTTCTTCACTTCGTTTTTACCATTTTTTTCCCATTATCTTCTTTCCCTAATCTCTGTGTGTTGTTTTTAGGTGTACGATGTGACTTCTTTTTTACCAGACCATCCAGGAGGCGATGAGCTCTTGCTACTCGCAGTTGGTGAGATGAAGATCATGCTGCCATTTTTTCAATAATACAATAATAACTCAAATTAGTAAAATACATGTAAAATTACCATGTTTTGAGCTAATACATTTTTTATTTAGCTCCTGCATTAATATTACCTTTTTTTTTTAGGAGAAGGCATTTCAATCTTAAGTAAATTAGGATAGGCTTAAAAGATATATATTTTTTTGGTTTAAGTCCCTTTTACAATTATAATTTGACTAAGGTGTTTCAAAAGTATTTTGAACAATAAAAAACAAAACAATAAAATCAAATGAAACCTGCCAATCTAGTTTCAATTGGCTATTTAAAATTTCCTTTAAAAATCACTCCACACTTCATAAAAAGAACCTCTTAAATTGATGTGGTTTTCAAGAAATTTTATTAAAAGTGATTATGATTAATTTAAATAAGTCGTTTTTAAATAGAAGGTGCGGATTAAAGCTGTGGTATGACTAATTGAGTTATGTTCGGGTTAACTTTGTTAAGATTTAACCAATAAATTTATTACATTTGAATTTGTAGAGAAAGATGCGACGATGGATTTCAAGAGTGTTGGACACAGCGAGTCAGCCCAAGAGCGGATGAAAAAGTATCGGATAGGAAAGATCGACAAGTCGACTCTTCCTGGGAAGCAAGCATATGTTGACAGTGGACATGTAGCAACTCCATCTCTTTTTTCTTTCTCGCCCTTGCATCTTCTAGTCCCCCTTTTGCTCCTTGCCTTGGCTTTTGCCTTTCCCTACTTTAAAGTTAAGGCCTAA

mRNA sequence

ATGGGTTCGAACGATAAAGTGTATACTTTCGATGAAGTTGCGAAGCACAACCACCAGAAGGATTGTTGGCTTATAATATCTGGAAATGTGTACGATGTGACTTCTTTTTTACCAGACCATCCAGGAGGCGATGAGCTCTTGCTACTCGCAGTTGAGAAAGATGCGACGATGGATTTCAAGAGTGTTGGACACAGCGAGTCAGCCCAAGAGCGGATGAAAAAGTATCGGATAGGAAAGATCGACAAGTCGACTCTTCCTGGGAAGCAAGCATATGTTGACAGTGGACATGTAGCAACTCCATCTCTTTTTTCTTTCTCGCCCTTGCATCTTCTAGTCCCCCTTTTGCTCCTTGCCTTGGCTTTTGCCTTTCCCTACTTTAAAGTTAAGGCCTAA

Coding sequence (CDS)

ATGGGTTCGAACGATAAAGTGTATACTTTCGATGAAGTTGCGAAGCACAACCACCAGAAGGATTGTTGGCTTATAATATCTGGAAATGTGTACGATGTGACTTCTTTTTTACCAGACCATCCAGGAGGCGATGAGCTCTTGCTACTCGCAGTTGAGAAAGATGCGACGATGGATTTCAAGAGTGTTGGACACAGCGAGTCAGCCCAAGAGCGGATGAAAAAGTATCGGATAGGAAAGATCGACAAGTCGACTCTTCCTGGGAAGCAAGCATATGTTGACAGTGGACATGTAGCAACTCCATCTCTTTTTTCTTTCTCGCCCTTGCATCTTCTAGTCCCCCTTTTGCTCCTTGCCTTGGCTTTTGCCTTTCCCTACTTTAAAGTTAAGGCCTAA

Protein sequence

MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFKSVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDSGHVATPSLFSFSPLHLLVPLLLLALAFAFPYFKVKA

Homology

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BLAST of Sgr011884 vs. NCBI nr
Match: XP_008459741.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 219.9 bits (559), Expect = 1.3e-53
Identity = 109/131 (83.21%), Postives = 118/131 (90.08%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M SN KVY+FDEVAKHNHQKDCWLIISG VYDVTSFLPDHPGGDELLLLAVEKDAT DFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDS-GHVATPSLFSFSPLHLLVPLLLLAL 120
           SVGHSE AQE+MK YRIGKID STLP KQ YV+S  +  TPSLFSFSP+H+L+P+LLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPYFKVKA 131
           AFAFPYFK+KA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Sgr011884 vs. NCBI nr
Match: XP_004140502.1 (cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cucumis sativus])

HSP 1 Score: 215.7 bits (548), Expect = 2.4e-52
Identity = 107/131 (81.68%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M SN KVY+FDEVAKHN QKDCWLIISG VYDVTSFLPDHPGGDELLLLAVEKDAT DFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDSG-HVATPSLFSFSPLHLLVPLLLLAL 120
           SVGHSE A E+MK Y+IGKID STLP KQ YV+S  +  TPSLFSFSP+HLL+P+LLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKVKA 131
           AFAFPYFK+KA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Sgr011884 vs. NCBI nr
Match: XP_022964486.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 210.3 bits (534), Expect = 9.9e-51
Identity = 103/131 (78.63%), Postives = 115/131 (87.79%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M SN KVYTFDEVAKHNH+KDCWLIISG VYDVTS+LPDHPGG ELL+LAVEKDAT DFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVD-SGHVATPSLFSFSPLHLLVPLLLLAL 120
           SVGHSESA+ERMKKY+IGKID  TLP +Q +V+ + H  TPSL SFSPLH+L+PLLLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKVKA 131
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of Sgr011884 vs. NCBI nr
Match: XP_038875836.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 209.5 bits (532), Expect = 1.7e-50
Identity = 101/131 (77.10%), Postives = 117/131 (89.31%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M S+ K+Y+FDEV+KHNH +DCWLIISG VYDVT+FLPDHPGG+ELLLLAV +DAT DFK
Sbjct: 3   MDSSSKLYSFDEVSKHNHHRDCWLIISGYVYDVTAFLPDHPGGEELLLLAVGRDATFDFK 62

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVD-SGHVATPSLFSFSPLHLLVPLLLLAL 120
           SVGHSE AQE+MKKYRIGKID STLP KQ YV+ + +  TPSLFSFSP+HLL+P+LLLAL
Sbjct: 63  SVGHSELAQEKMKKYRIGKIDMSTLPRKQQYVETTPYQPTPSLFSFSPIHLLIPILLLAL 122

Query: 121 AFAFPYFKVKA 131
           AFAFPYFK+KA
Sbjct: 123 AFAFPYFKLKA 133

[Close]

BLAST of Sgr011884 vs. NCBI nr
Match: KAG6593603.1 (Cycloartenol synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 207.6 bits (527), Expect = 6.4e-50
Identity = 100/131 (76.34%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M SN K++TFDEVAKHNH+KDCWLIISG+VYDVTS+LPDHPGG ELL+LAVEKDAT DFK
Sbjct: 584 MDSNSKLFTFDEVAKHNHEKDCWLIISGHVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 643

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVD-SGHVATPSLFSFSPLHLLVPLLLLAL 120
           SVGHSE+AQE MKKY+IGKID  TLP +Q +V+ + H  TPSL SFSPLH+L+PLLLL L
Sbjct: 644 SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 703

Query: 121 AFAFPYFKVKA 131
           AFAFPY+K+KA
Sbjct: 704 AFAFPYWKIKA 714

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BLAST of Sgr011884 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 132.1 bits (331), Expect = 4.5e-30
Identity = 69/134 (51.49%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M S+ KV +F+EV+KHN  KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDSGHVA-----TPSLFSFSPLHLLVPLL 120
            VGHS++A++ M KY IG+ID S++P  + YV     A     TP  F    L  LVP+L
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPE-FIIKILQFLVPIL 120

Query: 121 LLALAFAFPYFKVK 130
           +L LA    ++  K
Sbjct: 121 ILGLALVVRHYTKK 133

[Close]

BLAST of Sgr011884 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.0e-29
Identity = 69/134 (51.49%), Postives = 87/134 (64.93%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M S  KV  F+EV++HN  KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDSGHVA-----TPSLFSFSPLHLLVPLL 120
            VGHS++A++ M+KY IG+ID ST+P  + YV     A     TP  F    L  LVP+L
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPE-FMIKILQFLVPIL 120

Query: 121 LLALAFAFPYFKVK 130
           +L LA     +  K
Sbjct: 121 ILGLALVVRQYTKK 133

[Close]

BLAST of Sgr011884 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.1e-28
Identity = 64/130 (49.23%), Postives = 83/130 (63.85%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           MG   KV+T  EV++HN+ KDCWL+ISG VYDVT FL DHPGGDE+LL A  KDAT DF+
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDSG----HVATPSLFSFSPLHLLVPLLL 120
            VGHS SA+  + +Y +G ID +T+P K  Y        +    S F    L  LVPL++
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LALAFAFPYF 127
           L +AF   ++
Sbjct: 121 LGVAFGIRFY 130

[Close]

BLAST of Sgr011884 vs. ExPASy Swiss-Prot
Match: Q9ZWT2 (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.2e-28
Identity = 67/137 (48.91%), Postives = 89/137 (64.96%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           MG + KV+T  EV++H+  KDCW++I G VYDVT FL DHPGGDE++L +  KDAT DF+
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYV---DSGHVAT---PSLFSFSPLHLLVPL 120
            VGHS +A+  + +Y +G ID +T+P K  +V    +  VAT    S F    L  LVPL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LLLALAFAFPYF-KVKA 131
           L+L LAF   Y+ K KA
Sbjct: 121 LILGLAFGIRYYTKTKA 137

[Close]

BLAST of Sgr011884 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 6.1e-27
Identity = 60/124 (48.39%), Postives = 81/124 (65.32%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           MG   K++T  EV++HN   DCW++I+G VY+VT FL DHPGGD++LL +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDSG----HVATPSLFSFSPLHLLVPLLL 120
            VGHSESA+E M++Y +G+ID +T+P K  Y        +    S F    L  LVPL +
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

[Close]

BLAST of Sgr011884 vs. ExPASy TrEMBL
Match: A0A1S3CAE5 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 6.1e-54
Identity = 109/131 (83.21%), Postives = 118/131 (90.08%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M SN KVY+FDEVAKHNHQKDCWLIISG VYDVTSFLPDHPGGDELLLLAVEKDAT DFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDS-GHVATPSLFSFSPLHLLVPLLLLAL 120
           SVGHSE AQE+MK YRIGKID STLP KQ YV+S  +  TPSLFSFSP+H+L+P+LLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPYFKVKA 131
           AFAFPYFK+KA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Sgr011884 vs. ExPASy TrEMBL
Match: A0A0A0KA20 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G107940 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.1e-52
Identity = 107/131 (81.68%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M SN KVY+FDEVAKHN QKDCWLIISG VYDVTSFLPDHPGGDELLLLAVEKDAT DFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDSG-HVATPSLFSFSPLHLLVPLLLLAL 120
           SVGHSE A E+MK Y+IGKID STLP KQ YV+S  +  TPSLFSFSP+HLL+P+LLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKVKA 131
           AFAFPYFK+KA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Sgr011884 vs. ExPASy TrEMBL
Match: A0A6J1HJ10 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 4.8e-51
Identity = 103/131 (78.63%), Postives = 115/131 (87.79%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M SN KVYTFDEVAKHNH+KDCWLIISG VYDVTS+LPDHPGG ELL+LAVEKDAT DFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVD-SGHVATPSLFSFSPLHLLVPLLLLAL 120
           SVGHSESA+ERMKKY+IGKID  TLP +Q +V+ + H  TPSL SFSPLH+L+PLLLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKVKA 131
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of Sgr011884 vs. ExPASy TrEMBL
Match: A0A6J1KHQ2 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 5.9e-49
Identity = 98/131 (74.81%), Postives = 113/131 (86.26%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M SN K+YTF+EVAKHNH+KDCWLIISG VYDVTS+LPDHPGG ELL+L VEKDAT DFK
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVD-SGHVATPSLFSFSPLHLLVPLLLLAL 120
           SVGHSE+A+E MKKY+IGKID  TLP +Q YV+ + +  TPSL SFSPLH+L+PLLLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKVKA 131
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of Sgr011884 vs. ExPASy TrEMBL
Match: A0A5N5F465 (Cytochrome b5-like OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_000788 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.7e-35
Identity = 79/133 (59.40%), Postives = 95/133 (71.43%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           MGSN +VY FDEVAKHNH+KDCW+IISG VYDVT FL +HPGGDE+LLLAVEKDAT DF 
Sbjct: 1   MGSNSEVYLFDEVAKHNHKKDCWIIISGKVYDVTPFLEEHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDSGHVATP----SLFSFSPLHLLVPLLL 120
            VGHS+SA+E+M+KY +GK+D ST+P +  Y      A P    S      L  L+PLL+
Sbjct: 61  DVGHSDSAREQMEKYYVGKVDTSTIPAQPNYKLPPQAAAPAEQSSGLMVKLLQFLLPLLI 120

Query: 121 LALAFAFPYFKVK 130
           L  AFA  Y+  K
Sbjct: 121 LGAAFALQYYNKK 133

[Close]

BLAST of Sgr011884 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 132.1 bits (331), Expect = 3.2e-31
Identity = 69/134 (51.49%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           M S+ KV +F+EV+KHN  KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDSGHVA-----TPSLFSFSPLHLLVPLL 120
            VGHS++A++ M KY IG+ID S++P  + YV     A     TP  F    L  LVP+L
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPE-FIIKILQFLVPIL 120

Query: 121 LLALAFAFPYFKVK 130
           +L LA    ++  K
Sbjct: 121 ILGLALVVRHYTKK 133

[Close]

BLAST of Sgr011884 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 125.6 bits (314), Expect = 3.0e-29
Identity = 67/137 (48.91%), Postives = 89/137 (64.96%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           MG + KV+T  EV++H+  KDCW++I G VYDVT FL DHPGGDE++L +  KDAT DF+
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYV---DSGHVAT---PSLFSFSPLHLLVPL 120
            VGHS +A+  + +Y +G ID +T+P K  +V    +  VAT    S F    L  LVPL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LLLALAFAFPYF-KVKA 131
           L+L LAF   Y+ K KA
Sbjct: 121 LILGLAFGIRYYTKTKA 137

[Close]

BLAST of Sgr011884 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 121.7 bits (304), Expect = 4.3e-28
Identity = 60/124 (48.39%), Postives = 81/124 (65.32%), Query Frame = 0


Query: 1   MGSNDKVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFK 60
           MG   K++T  EV++HN   DCW++I+G VY+VT FL DHPGGD++LL +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSESAQERMKKYRIGKIDKSTLPGKQAYVDSG----HVATPSLFSFSPLHLLVPLLL 120
            VGHSESA+E M++Y +G+ID +T+P K  Y        +    S F    L  LVPL +
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

[Close]

BLAST of Sgr011884 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 110.9 bits (276), Expect = 7.6e-25
Identity = 56/127 (44.09%), Postives = 83/127 (65.35%), Query Frame = 0


Query: 9   TFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFKSVGHSESA 68
           +F +VAKH  + DCW++I G VYD+++F+ +HPGGD +LL    KDA++DF+ V HS+ A
Sbjct: 6   SFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDA 65

Query: 69  QERMKKYRIGKIDKSTLPGKQAYV-----DSGHVATPSLFSFSPLHL-LVPLLLLALAFA 128
           +E MKKY IG +D+ST+P  Q Y+     +S    T    S   L + L+PLL+L +AFA
Sbjct: 66  KELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVAFA 125

Query: 129 FPYFKVK 130
             ++  K
Sbjct: 126 LRFYNNK 132

[Close]

BLAST of Sgr011884 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 108.6 bits (270), Expect = 3.8e-24
Identity = 51/121 (42.15%), Postives = 79/121 (65.29%), Query Frame = 0


Query: 6   KVYTFDEVAKHNHQKDCWLIISGNVYDVTSFLPDHPGGDELLLLAVEKDATMDFKSVGHS 65
           K+Y+ +E A HN Q DCW++I G VYDV+S++ +HPGGD++LL    KDAT DF+  GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  ESAQERMKKYRIGKIDKSTLPG----KQAYVDSGHVATPSLFSFSPLHLLVPLLLLALAF 123
           + A+E M+KY IG++D+S+LP     K    D    +   LF  +  + +VP+ ++ ++ 
Sbjct: 66  KDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSIITISV 125

The following BLAST results are available for this feature:

BLAST of Sgr011884 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459741.1	1.3e-53	83.21	PREDICTED: cytochrome b5 [Cucumis melo]	[more]
XP_004140502.1	2.4e-52	81.68	cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cuc...	[more]
XP_022964486.1	9.9e-51	78.63	cytochrome b5-like [Cucurbita moschata]	[more]
XP_038875836.1	1.7e-50	77.10	cytochrome b5-like [Benincasa hispida]	[more]
KAG6593603.1	6.4e-50	76.34	Cycloartenol synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Sgr011884 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42342	4.5e-30	51.49	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P40934	1.0e-29	51.49	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49098	1.1e-28	49.23	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q9ZWT2	4.2e-28	48.91	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]
O48845	6.1e-27	48.39	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]

BLAST of Sgr011884 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CAE5	6.1e-54	83.21	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1	[more]
A0A0A0KA20	1.1e-52	81.68	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1HJ10	4.8e-51	78.63	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1	[more]
A0A6J1KHQ2	5.9e-49	74.81	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1	[more]
A0A5N5F465	1.7e-35	59.40	Cytochrome b5-like OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_000...	[more]

BLAST of Sgr011884 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53560.1	3.2e-31	51.49	cytochrome B5 isoform E	[more]
AT5G48810.1	3.0e-29	48.91	cytochrome B5 isoform D	[more]
AT2G32720.1	4.3e-28	48.39	cytochrome B5 isoform B	[more]
AT2G46650.1	7.6e-25	44.09	cytochrome B5 isoform C	[more]
AT1G26340.1	3.8e-24	42.15	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 40..54 score: 44.55 coord: 55..62 score: 35.8 coord: 30..40 score: 64.74 coord: 68..80 score: 36.13
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 6.4E-27 score: 105.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 10..80 e-value: 3.7E-23 score: 81.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 27.681459
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..86 e-value: 3.7E-33 score: 115.3
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..85
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..128
None	No IPR available	PANTHER	PTHR19359:SF81	SUBFAMILY NOT NAMED	coord: 1..128
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr011884.1	Sgr011884.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding