Sgr011635 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr011635 vs. NCBI nr
Match: XP_022141405.1 (DIS3-like exonuclease 2 isoform X2 [Momordica charantia])

HSP 1 Score: 1968.0 bits (5097), Expect = 0.0e+00
Identity = 987/1124 (87.81%), Postives = 1042/1124 (92.70%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKKRRSNRRSK--QNASITTSVSCSSVNAIPGEASECMEN 60
            MR AVEQ T ERN+DGEKEK+KKRRSNRRSK  Q ASITT+VSCSSVN IPGE SECMEN
Sbjct: 1    MRAAVEQSTSERNEDGEKEKRKKRRSNRRSKQTQTASITTAVSCSSVNEIPGETSECMEN 60

Query: 61   GRIDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFI 120
            GRID NLTA +NYSS  +  Y SN+P EHGLTRTNKIA SSLPPLHISEQ +  ESQ+ I
Sbjct: 61   GRIDANLTAPTNYSSLTEPAYRSNNPTEHGLTRTNKIAFSSLPPLHISEQAKFSESQNLI 120

Query: 121  NQHLHSSDAGGKFIKSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVN 180
            NQH HSS+AGG+ IKSCPQ+IA GR+PGIS NQ+S PAH TENN QRKYFTS+W MDDVN
Sbjct: 121  NQHFHSSNAGGRIIKSCPQEIASGRVPGISANQNSLPAHVTENNSQRKYFTSHWSMDDVN 180

Query: 181  EGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTS 240
            EGLQKGDIF A FRVNAHN LEAYCKIDGLPVDVLINGIASQNRAVEGD VAIKVDPFTS
Sbjct: 181  EGLQKGDIFIALFRVNAHNGLEAYCKIDGLPVDVLINGIASQNRAVEGDTVAIKVDPFTS 240

Query: 241  WTRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKRCC 300
            WTRMKG +EAHNNMH MEDAN+HAEEN KDGH+C+ KNKVDV VKS++FRSSSLPDKRCC
Sbjct: 241  WTRMKGASEAHNNMHSMEDANIHAEENGKDGHNCEEKNKVDV-VKSNNFRSSSLPDKRCC 300

Query: 301  SDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYP 360
            S++N VLDGTAC D LL NYEQ DVYQS VLDSSQAHYS NQDDVSKAIG+ICAVINL+P
Sbjct: 301  SEENKVLDGTAC-DVLLSNYEQSDVYQSLVLDSSQAHYSCNQDDVSKAIGKICAVINLHP 360

Query: 361  SKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMP 420
            SKRP GRVVAILE S QRE+IVGHL VKKFLSFQEIY+KEMN KSCL SS N GYVQLMP
Sbjct: 361  SKRPTGRVVAILENSLQRESIVGHLIVKKFLSFQEIYMKEMNTKSCLPSSPNHGYVQLMP 420

Query: 421  NDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRG 480
            NDARFP+MMVL  DLP+ IKKRLDNGDVTVESELVAARIHEWV+ESSAP+AHVLHVLG+G
Sbjct: 421  NDARFPMMMVLTEDLPDHIKKRLDNGDVTVESELVAARIHEWVQESSAPRAHVLHVLGQG 480

Query: 481  SGVESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDPSSA 540
            S VESH+DAILF+NAIRTCEFSHDSLSCLPHTPWKIP +ELQ RRDLRNLCIFTIDPS+A
Sbjct: 481  SEVESHVDAILFQNAIRTCEFSHDSLSCLPHTPWKIPPDELQCRRDLRNLCIFTIDPSTA 540

Query: 541  SDLDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPL 600
            SDLDDALSVEKLANGIFRVGIHIADVSHFVLP+TALDKEAQIRST  YLLQRKIPMLPPL
Sbjct: 541  SDLDDALSVEKLANGIFRVGIHIADVSHFVLPETALDKEAQIRSTCFYLLQRKIPMLPPL 600

Query: 601  LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDS 660
            LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGL+DS
Sbjct: 601  LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLLDS 660

Query: 661  ASSKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYDEF 720
              SKI  NHCP LHGQFAWP VISSVKIL+EISKTLKEKRFRDGALRL+NSKRVYLYDE+
Sbjct: 661  DCSKISRNHCPHLHGQFAWPDVISSVKILHEISKTLKEKRFRDGALRLDNSKRVYLYDEY 720

Query: 721  GMPYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELF 780
            G+PYDS FYEHKDSNFLVEEFMLLANTTVAEV+SRTFPDSALLRRHPEP+MRKLREFE F
Sbjct: 721  GIPYDSKFYEHKDSNFLVEEFMLLANTTVAEVVSRTFPDSALLRRHPEPVMRKLREFESF 780

Query: 781  CSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKDGE 840
            CS+HGF LDTSSSVQFQ+SLEQIR KLHDDPLLFDILISYATRPMQLATYFCSGELKDGE
Sbjct: 781  CSKHGFELDTSSSVQFQQSLEQIRKKLHDDPLLFDILISYATRPMQLATYFCSGELKDGE 840

Query: 841  NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF 900
            NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF
Sbjct: 841  NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF 900

Query: 901  TGIHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYM 960
            TGIHFDKDAADSLEGREALS+AAL+HGVPCTKLLSDVALHCNNRKLAS+HVADACDKLYM
Sbjct: 901  TGIHFDKDAADSLEGREALSAAALKHGVPCTKLLSDVALHCNNRKLASKHVADACDKLYM 960

Query: 961  WALLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTLVLSF 1020
            WALLKKKEIL SDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDA+STLVLSF
Sbjct: 961  WALLKKKEILLSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDASSTLVLSF 1020

Query: 1021 FGTRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGAS-TGGAAVEQESN 1080
            FGTRRS++ RGSSKWKALE+VALVISPCDLNVQ+R LGGSPSE GG S T GA VEQESN
Sbjct: 1021 FGTRRSFKSRGSSKWKALEEVALVISPCDLNVQERILGGSPSESGGXSTTEGATVEQESN 1080

Query: 1081 LKSHISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            LKSH SDTGI PAVFPLTVRL S+IPVALHA+GGDDGPIDIGVR
Sbjct: 1081 LKSHTSDTGIVPAVFPLTVRLFSTIPVALHAIGGDDGPIDIGVR 1122

BLAST of Sgr011635 vs. NCBI nr
Match: XP_022141403.1 (DIS3-like exonuclease 2 isoform X1 [Momordica charantia])

HSP 1 Score: 1968.0 bits (5097), Expect = 0.0e+00
Identity = 987/1124 (87.81%), Postives = 1042/1124 (92.70%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKKRRSNRRSK--QNASITTSVSCSSVNAIPGEASECMEN 60
            MR AVEQ T ERN+DGEKEK+KKRRSNRRSK  Q ASITT+VSCSSVN IPGE SECMEN
Sbjct: 1    MRAAVEQSTSERNEDGEKEKRKKRRSNRRSKQTQTASITTAVSCSSVNEIPGETSECMEN 60

Query: 61   GRIDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFI 120
            GRID NLTA +NYSS  +  Y SN+P EHGLTRTNKIA SSLPPLHISEQ +  ESQ+ I
Sbjct: 61   GRIDANLTAPTNYSSLTEPAYRSNNPTEHGLTRTNKIAFSSLPPLHISEQAKFSESQNLI 120

Query: 121  NQHLHSSDAGGKFIKSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVN 180
            NQH HSS+AGG+ IKSCPQ+IA GR+PGIS NQ+S PAH TENN QRKYFTS+W MDDVN
Sbjct: 121  NQHFHSSNAGGRIIKSCPQEIASGRVPGISANQNSLPAHVTENNSQRKYFTSHWSMDDVN 180

Query: 181  EGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTS 240
            EGLQKGDIF A FRVNAHN LEAYCKIDGLPVDVLINGIASQNRAVEGD VAIKVDPFTS
Sbjct: 181  EGLQKGDIFIALFRVNAHNGLEAYCKIDGLPVDVLINGIASQNRAVEGDTVAIKVDPFTS 240

Query: 241  WTRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKRCC 300
            WTRMKG +EAHNNMH MEDAN+HAEEN KDGH+C+ KNKVDV VKS++FRSSSLPDKRCC
Sbjct: 241  WTRMKGASEAHNNMHSMEDANIHAEENGKDGHNCEEKNKVDV-VKSNNFRSSSLPDKRCC 300

Query: 301  SDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYP 360
            S++N VLDGTAC D LL NYEQ DVYQS VLDSSQAHYS NQDDVSKAIG+ICAVINL+P
Sbjct: 301  SEENKVLDGTAC-DVLLSNYEQSDVYQSLVLDSSQAHYSCNQDDVSKAIGKICAVINLHP 360

Query: 361  SKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMP 420
            SKRP GRVVAILE S QRE+IVGHL VKKFLSFQEIY+KEMN KSCL SS N GYVQLMP
Sbjct: 361  SKRPTGRVVAILENSLQRESIVGHLIVKKFLSFQEIYMKEMNTKSCLPSSPNHGYVQLMP 420

Query: 421  NDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRG 480
            NDARFP+MMVL  DLP+ IKKRLDNGDVTVESELVAARIHEWV+ESSAP+AHVLHVLG+G
Sbjct: 421  NDARFPMMMVLTEDLPDHIKKRLDNGDVTVESELVAARIHEWVQESSAPRAHVLHVLGQG 480

Query: 481  SGVESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDPSSA 540
            S VESH+DAILF+NAIRTCEFSHDSLSCLPHTPWKIP +ELQ RRDLRNLCIFTIDPS+A
Sbjct: 481  SEVESHVDAILFQNAIRTCEFSHDSLSCLPHTPWKIPPDELQCRRDLRNLCIFTIDPSTA 540

Query: 541  SDLDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPL 600
            SDLDDALSVEKLANGIFRVGIHIADVSHFVLP+TALDKEAQIRST  YLLQRKIPMLPPL
Sbjct: 541  SDLDDALSVEKLANGIFRVGIHIADVSHFVLPETALDKEAQIRSTCFYLLQRKIPMLPPL 600

Query: 601  LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDS 660
            LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGL+DS
Sbjct: 601  LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLLDS 660

Query: 661  ASSKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYDEF 720
              SKI  NHCP LHGQFAWP VISSVKIL+EISKTLKEKRFRDGALRL+NSKRVYLYDE+
Sbjct: 661  DCSKISRNHCPHLHGQFAWPDVISSVKILHEISKTLKEKRFRDGALRLDNSKRVYLYDEY 720

Query: 721  GMPYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELF 780
            G+PYDS FYEHKDSNFLVEEFMLLANTTVAEV+SRTFPDSALLRRHPEP+MRKLREFE F
Sbjct: 721  GIPYDSKFYEHKDSNFLVEEFMLLANTTVAEVVSRTFPDSALLRRHPEPVMRKLREFESF 780

Query: 781  CSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKDGE 840
            CS+HGF LDTSSSVQFQ+SLEQIR KLHDDPLLFDILISYATRPMQLATYFCSGELKDGE
Sbjct: 781  CSKHGFELDTSSSVQFQQSLEQIRKKLHDDPLLFDILISYATRPMQLATYFCSGELKDGE 840

Query: 841  NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF 900
            NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF
Sbjct: 841  NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF 900

Query: 901  TGIHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYM 960
            TGIHFDKDAADSLEGREALS+AAL+HGVPCTKLLSDVALHCNNRKLAS+HVADACDKLYM
Sbjct: 901  TGIHFDKDAADSLEGREALSAAALKHGVPCTKLLSDVALHCNNRKLASKHVADACDKLYM 960

Query: 961  WALLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTLVLSF 1020
            WALLKKKEIL SDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDA+STLVLSF
Sbjct: 961  WALLKKKEILLSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDASSTLVLSF 1020

Query: 1021 FGTRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGAS-TGGAAVEQESN 1080
            FGTRRS++ RGSSKWKALE+VALVISPCDLNVQ+R LGGSPSE GG S T GA VEQESN
Sbjct: 1021 FGTRRSFKSRGSSKWKALEEVALVISPCDLNVQERILGGSPSESGGXSTTEGATVEQESN 1080

Query: 1081 LKSHISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            LKSH SDTGI PAVFPLTVRL S+IPVALHA+GGDDGPIDIGVR
Sbjct: 1081 LKSHTSDTGIVPAVFPLTVRLFSTIPVALHAIGGDDGPIDIGVR 1122

BLAST of Sgr011635 vs. NCBI nr
Match: XP_038886229.1 (DIS3-like exonuclease 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 1939.5 bits (5023), Expect = 0.0e+00
Identity = 974/1121 (86.89%), Postives = 1033/1121 (92.15%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKKRRSNRRSKQNASITTSVSCSSVNAIPGEASECMENGR 60
            MRGAVEQ TP+RN+DG+KEKKKKRRSNRRSKQNAS++TS SC+SVN I GEASE MENGR
Sbjct: 1    MRGAVEQSTPDRNEDGDKEKKKKRRSNRRSKQNASLSTSASCNSVNGITGEASESMENGR 60

Query: 61   IDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFINQ 120
            ID NLT+ SNYSS  QQ Y SNHP EHGLTR NKIA SSLPPLHISE+ EL ESQ+  NQ
Sbjct: 61   IDANLTSPSNYSSLTQQAYQSNHPIEHGLTRRNKIAFSSLPPLHISEEAELSESQNLKNQ 120

Query: 121  HLHSSDAGGKFIKSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVNEG 180
            +LHS D GG+ IKSCP+QIA GR  GIS+NQHSPPA  TENN QRKYF S+W MDDVNEG
Sbjct: 121  NLHSLDDGGRIIKSCPEQIAFGRNSGISLNQHSPPADVTENNSQRKYFPSHWSMDDVNEG 180

Query: 181  LQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTSWT 240
            LQKGDIFKA FRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTSWT
Sbjct: 181  LQKGDIFKALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTSWT 240

Query: 241  RMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKRCCSD 300
            RMKGT+EAHNNMH MED NL  E  EKD H+CKGKNKVD DVKSDSFRSSSLPDKRCCS+
Sbjct: 241  RMKGTSEAHNNMHSMEDVNLPFEVAEKDCHNCKGKNKVDADVKSDSFRSSSLPDKRCCSE 300

Query: 301  DNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYPSK 360
            D  VLDGTAC DDLL NYEQCDV QSSV+  SQAH+SSNQDDVSKA+GRICAVINLYP+K
Sbjct: 301  DK-VLDGTAC-DDLLSNYEQCDVNQSSVVYPSQAHFSSNQDDVSKAVGRICAVINLYPAK 360

Query: 361  RPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMPND 420
            RP GRVV ILEKSR RE +VGHLNVKKFLSFQEIY+KE  K   LS   N GYVQL+PND
Sbjct: 361  RPTGRVVTILEKSRLRETVVGHLNVKKFLSFQEIYVKENTK--FLSPLQNCGYVQLIPND 420

Query: 421  ARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRGSG 480
            ARFP+MMVLA DLP+CIKKRLDNGD+TVESELVAARIHEWV ESSAP+A VLHVLGRGS 
Sbjct: 421  ARFPIMMVLAEDLPDCIKKRLDNGDLTVESELVAARIHEWVIESSAPRAQVLHVLGRGSE 480

Query: 481  VESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDPSSASD 540
            VESHIDAILFENAIRTCEFSHDSLSC+PHTPWKIPQEELQ RRD+RNLCIFTIDPSSASD
Sbjct: 481  VESHIDAILFENAIRTCEFSHDSLSCIPHTPWKIPQEELQCRRDIRNLCIFTIDPSSASD 540

Query: 541  LDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
            LDDALSV+ LANGIFRVGIH+ADVSHFVLP TALDKEAQIRS SVYLLQRKIPMLPPLLS
Sbjct: 541  LDDALSVQILANGIFRVGIHVADVSHFVLPGTALDKEAQIRSMSVYLLQRKIPMLPPLLS 600

Query: 601  ENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDSAS 660
            ENIGSLNPGVDRLAFSLFLDIN+CGDVK CWIGRTVICSCCKLSYE AQDIIDGLIDS S
Sbjct: 601  ENIGSLNPGVDRLAFSLFLDINNCGDVKECWIGRTVICSCCKLSYEQAQDIIDGLIDSDS 660

Query: 661  SKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYDEFGM 720
            SKIL N+CPQLHGQFAW  VISSVK+L+EISKTLKEKRFRDGALRLENSK ++LYDE G+
Sbjct: 661  SKILRNNCPQLHGQFAWHDVISSVKLLHEISKTLKEKRFRDGALRLENSKIIFLYDECGI 720

Query: 721  PYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELFCS 780
            PYDSTFYE KDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHP+PI+RKLREFE FCS
Sbjct: 721  PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPDPILRKLREFESFCS 780

Query: 781  RHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKDGENG 840
            RHGF LDTSSSVQFQ+SLEQIR+KLHDDPLLFDIL SYATRPMQLATYFCSGELKDGE G
Sbjct: 781  RHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCFTG 900
            SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAE LYLKH+GIIQKVN DEQMRCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEMLYLKHRGIIQKVNSDEQMRCFTG 900

Query: 901  IHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYMWA 960
            I FDKDAADSLEGREALSSAALRHGVPC+KLLSDVA+HCNNRKLAS+HVAD C+KLYMWA
Sbjct: 901  ISFDKDAADSLEGREALSSAALRHGVPCSKLLSDVAVHCNNRKLASKHVADGCEKLYMWA 960

Query: 961  LLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTLVLSFFG 1020
            LLKKK+ILFSDARVLGLGPRFMS+YIQKLAIERRIYYDEVEGLAVEWLD TSTLVLSFFG
Sbjct: 961  LLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLDTTSTLVLSFFG 1020

Query: 1021 TRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGASTGGAAVEQESNLKS 1080
            TRRS+R RGS KWKALEDVAL+ISPCD N++QRTLG SPSELGGA+TGG+AVEQESNLKS
Sbjct: 1021 TRRSHRNRGSIKWKALEDVALIISPCDQNIKQRTLGVSPSELGGATTGGSAVEQESNLKS 1080

Query: 1081 HISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            H+SDTG+DPAVFPLTVRLLS+IPVALHAVGGDDGPIDIGVR
Sbjct: 1081 HVSDTGVDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVR 1117

BLAST of Sgr011635 vs. NCBI nr
Match: KAG7016503.1 (DIS3-like exonuclease 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1932.9 bits (5006), Expect = 0.0e+00
Identity = 967/1121 (86.26%), Postives = 1035/1121 (92.33%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKKRRSNRRSKQNASITTSVSCSSVNAIPGEASECMENGR 60
            MRGAVEQ TPER DDG+KEKKKKRRSNRRSKQNASI+TSVSCSSVN + GEASECMENG+
Sbjct: 1    MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60

Query: 61   IDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFINQ 120
            ID NLTA SN+SS  QQ + SNHP EHG+TR NKIA SSLPPLHISEQ EL ESQ+ IN+
Sbjct: 61   IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120

Query: 121  HLHSSDAGGKFIKSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVNEG 180
            +LH  DAGGK IKSCP+QI CGRMPGIS+NQHSPPA  TENN QRKYF S+W ++DV+EG
Sbjct: 121  NLHPLDAGGKTIKSCPEQIVCGRMPGISINQHSPPADVTENNTQRKYFASHWSVEDVDEG 180

Query: 181  LQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTSWT 240
            LQKGDIF+AFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAI VDP TSW 
Sbjct: 181  LQKGDIFRAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTSWI 240

Query: 241  RMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKRCCSD 300
            RMKGT+E HN+ H MEDANL AE  E DG +CKGKNK+D  VKSDSFRSSS PDKRCCS+
Sbjct: 241  RMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKLDASVKSDSFRSSSSPDKRCCSE 300

Query: 301  DNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYPSK 360
            D I LDGTACDD LL N EQ DVYQSSV+D  +AHYSSNQDDVSKAI RICAVI+ YP K
Sbjct: 301  DKI-LDGTACDDLLLKN-EQRDVYQSSVVDPPEAHYSSNQDDVSKAIQRICAVISSYPGK 360

Query: 361  RPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMPND 420
            RP GRVVAILEKSRQRE+IVGHLNVKKFLSFQEIY+KEMN KSCLS S N G+VQLMPND
Sbjct: 361  RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGHVQLMPND 420

Query: 421  ARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRGSG 480
            ARFP+MMVLAGDLP+ IKKRLDNGDVTVE+ELVA +IHEWV+ESSAPQAHVLHVLGRGS 
Sbjct: 421  ARFPIMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQAHVLHVLGRGSE 480

Query: 481  VESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDPSSASD 540
            V SHIDAILFENAI +CEFS+DSL+CLPH+PWKIP EELQ RRDLRNLCIFTIDPSSASD
Sbjct: 481  VASHIDAILFENAIHSCEFSNDSLACLPHSPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540

Query: 541  LDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
            LDDALSV+KLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS
Sbjct: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDSAS 660
            EN+GSL+PGVDRLAFSLFLDI++CGDVK+ WIGRTVICSCCKLSYEHAQDIIDGLIDS S
Sbjct: 601  ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660

Query: 661  SKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYDEFGM 720
            SK LGN+ PQLHGQFAWP VISSVK+L+EISKTLK+KRFRDGALRLENSK VYLYDE+G+
Sbjct: 661  SKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGV 720

Query: 721  PYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELFCS 780
            PYDSTFYE KDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPI+RKLREFE FCS
Sbjct: 721  PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780

Query: 781  RHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKDGENG 840
            +HGF LDTSSSVQFQ+SLEQIR+KLHDDPLLFDIL SYATRPMQLATYFCSGELKDGE G
Sbjct: 781  KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCFTG 900
            SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAE+LYLKH+GIIQKVN DEQ+RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGIIQKVNSDEQIRCFTG 900

Query: 901  IHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYMWA 960
            ++FDKDAADSLEGREALSSAALRHGVPC KLL+DVALHCNNRKLAS+HVAD CDKLYMWA
Sbjct: 901  MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960

Query: 961  LLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTLVLSFFG 1020
            LLKKK++LFSDARVLGLGPRFMSLYIQKL IERRIYYDE EGLAVEWL+ TSTLVLSFFG
Sbjct: 961  LLKKKKVLFSDARVLGLGPRFMSLYIQKLDIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020

Query: 1021 TRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGASTGGAAVEQESNLKS 1080
            TRRS+R RGS KWKALEDVALV+SPCD NV+QR LG SPSELGG STGGA VEQESNLKS
Sbjct: 1021 TRRSHRSRGSIKWKALEDVALVVSPCDHNVKQRALGVSPSELGGTSTGGAVVEQESNLKS 1080

Query: 1081 HISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            H+SDTGIDPAVFPLTVRLLS+IPVALHAVGGDDGPIDIGVR
Sbjct: 1081 HVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVR 1119

BLAST of Sgr011635 vs. NCBI nr
Match: KAG6578979.1 (DIS3-like exonuclease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1927.9 bits (4993), Expect = 0.0e+00
Identity = 966/1122 (86.10%), Postives = 1034/1122 (92.16%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKKRRSNRRSKQNASITTSVSCSSVNAIPGEASECMENGR 60
            MRGAVEQ TPER DDG+KEKKKKRRSNRRSKQNASI+TSVSCSSVN + GEASECMENG+
Sbjct: 1    MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60

Query: 61   IDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFINQ 120
            ID NLTA SN+SS  QQ + SNHP EHG+TR NKIA SSLPPLHISEQ EL ESQ+ IN+
Sbjct: 61   IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120

Query: 121  HLHSSDAGGKFIKSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVNEG 180
            +LH  DAGGK IKSCP+QI CGRMPGIS+NQHSPPA  TENN QRKYF S+W ++DV+EG
Sbjct: 121  NLHPLDAGGKTIKSCPEQIVCGRMPGISINQHSPPADVTENNTQRKYFASHWSVEDVDEG 180

Query: 181  LQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTSWT 240
            LQKGDIF+AFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAI VDP TSW 
Sbjct: 181  LQKGDIFRAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTSWI 240

Query: 241  RMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKRCCSD 300
            RMKGT+E HN+ H MEDANL AE  E DG +CKGKNK D  VKSDSFRSSS PDKRCCS+
Sbjct: 241  RMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKFDASVKSDSFRSSSSPDKRCCSE 300

Query: 301  DNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYPSK 360
            D I LDGTACDD LL N EQ DVYQSSV+D  +AHYSSNQDDVSKAI RICAVI+ YP K
Sbjct: 301  DKI-LDGTACDDLLLKN-EQRDVYQSSVVDPPEAHYSSNQDDVSKAIQRICAVISSYPGK 360

Query: 361  RPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMPND 420
            RP GRVVAILEKSRQRE+IVGHLNVKKFLSFQEIY+KEMN KSCLS S N G+VQLMPND
Sbjct: 361  RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGHVQLMPND 420

Query: 421  ARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRGSG 480
            ARFP+MMVLAGDLP+ IKKRLDNGDVTVE+ELVA +IHEWV+ESSAPQAHVLHVLGRGS 
Sbjct: 421  ARFPIMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQAHVLHVLGRGSE 480

Query: 481  VESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDPSSASD 540
            V SHIDAILFENAI +CEFS+DSL+CLPH+PWKIP EELQ RRDLRNLCIFTIDPSSASD
Sbjct: 481  VASHIDAILFENAIHSCEFSNDSLACLPHSPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540

Query: 541  LDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
            LDDALSV+KLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS
Sbjct: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDSAS 660
            EN+GSL+PGVDRLAFSLFLDI++CGDVK+ WIGRTVICSCCKLSYEHAQDIIDGLIDS S
Sbjct: 601  ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660

Query: 661  SKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYDEFGM 720
            SK LGN+ PQLHGQFAWP VISSVK+L+EISKTLK+KRFRDGALRLENSK VYLYDE+G+
Sbjct: 661  SKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGV 720

Query: 721  PYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELFCS 780
            PYDSTFYE KDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPI+RKLREFE FCS
Sbjct: 721  PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780

Query: 781  RHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKDGENG 840
            +HGF LDTSSSVQFQ+SLEQIR+KLHDDPLLFDIL SYATRPMQLATYFCSGELKDGE G
Sbjct: 781  KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCFTG 900
            SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAE+LYLKH+GIIQKVN DEQ+RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGIIQKVNSDEQIRCFTG 900

Query: 901  IHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYMWA 960
            ++FDKDAADSLEGREALSSAALRHGVPC KLL+DVALHCNNRKLAS+HVAD CDKLYMWA
Sbjct: 901  MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960

Query: 961  LLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTLVLSFFG 1020
            LLKKK++LFSDARVLGLGPRFMSL IQKLAIERRIYYDE EGLAVEWL+ TSTLVLSFFG
Sbjct: 961  LLKKKKVLFSDARVLGLGPRFMSLDIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020

Query: 1021 TRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGASTGGAAVEQESNLKS 1080
            TRRS+R RGS KWKALEDVALV+SPCD NV+QR LG SPSELGG STGGA VEQESNLKS
Sbjct: 1021 TRRSHRSRGSIKWKALEDVALVVSPCDHNVKQRALGVSPSELGGTSTGGAVVEQESNLKS 1080

Query: 1081 HISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVRS 1123
            H+SDTGIDPAVFPLTVRLLS+IPVALHAVGGDDGPIDIG+ S
Sbjct: 1081 HVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGMCS 1120

BLAST of Sgr011635 vs. ExPASy Swiss-Prot
Match: P0DM58 (DIS3-like exonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=SOV PE=1 SV=1)

HSP 1 Score: 991.5 bits (2562), Expect = 1.0e-287
Identity = 565/1127 (50.13%), Postives = 738/1127 (65.48%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKK-RRSNRRSKQNASITTSVSCSSVNAIPGEASECMENG 60
            M+ A  + + ER ++G K+K+ + ++ NRRSKQ          SSV        E ++ G
Sbjct: 1    MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQ----------SSVPIEDAHVEESLD-G 60

Query: 61   RIDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFIN 120
            R  +   A  + SSS QQ     + +E    R + +A +S+PP+  +E G    S S + 
Sbjct: 61   RDSSRSKAKDSTSSSKQQR---PNTDELEAMRASNVAFNSMPPMR-AESGYPRRSASPL- 120

Query: 121  QHLHSSDAGGKFI-KSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVN 180
              L S +   + + KSCP   AC + PG+    +     + E + QRK F+S+W +D V 
Sbjct: 121  --LSSPEVSKQLLSKSCPDPRACEQSPGM----NGELFQQIEGSSQRKIFSSHWSLDAVT 180

Query: 181  EGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTS 240
            E L+KG+ FKA FRVNAHNR EAYCKIDG+P D+LING   Q+RAVEGD V IK+DP + 
Sbjct: 181  EALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKLDPLSL 240

Query: 241  WTRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVD-VDVKSDSFRSSSLPDKRC 300
            W +MKG      +    E  N      EKD    + KN +D V+   D F          
Sbjct: 241  WPKMKGFVT--ESAAKPEGTN---SPPEKDDKKARQKNGIDVVEGFEDGF---------- 300

Query: 301  CSDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLY 360
             S +   + G    + + P+           LDS    +   + + S A+ ++C +++ +
Sbjct: 301  -SKNKSSVIGKGAKNGVTPS-------SPPSLDSCLGSFCEQKGNCS-AVDKLCGILSSF 360

Query: 361  PSKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLS--SSSNQGYVQ 420
            P KRP G+VVA++EKS  R++IVG L+VK +     I+ KE + K C S  S S+  YVQ
Sbjct: 361  PHKRPTGQVVAVVEKSLVRDSIVGLLDVKGW-----IHYKESDPKRCKSPLSLSDDEYVQ 420

Query: 421  LMPNDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVL 480
            LMP D RFP ++V    LP  I+ RL+N D  +E+ELVAA+I +W E S  P A + H+ 
Sbjct: 421  LMPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLF 480

Query: 481  GRGSGVESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDP 540
            GRGS +E  I+AIL++N++   +FS  SL+ LP  PW++P+EE+Q R+DLR+LC+ TIDP
Sbjct: 481  GRGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDP 540

Query: 541  SSASDLDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPML 600
            S+A+DLDDALSV+ L  G FRVG+HIADVS+FVLP+TALD EA+ RSTSVYL+QRKI ML
Sbjct: 541  STATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISML 600

Query: 601  PPLLSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGL 660
            PPLLSEN+GSL+PG DRLAFS+  D+N  GDV + WIGRT+I SCCKLSY+HAQDIIDG 
Sbjct: 601  PPLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDGK 660

Query: 661  IDSASSKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLY 720
             D A      N  P LHG F W  V  SVK L EIS TL++KRFR+GAL+LENSK V+L+
Sbjct: 661  SDVAE-----NGWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLF 720

Query: 721  DEFGMPYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREF 780
            DE G+PYD      K SNFLVEEFMLLAN T AEVIS+ +P S+LLRRHPEP  RKL+EF
Sbjct: 721  DEHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYPASSLLRRHPEPNTRKLKEF 780

Query: 781  ELFCSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELK 840
            E FCS+HG  LD SSS Q Q SLE+I   L DD +  DIL +YA +PMQLA+YFC+G LK
Sbjct: 781  EGFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLK 840

Query: 841  DG-ENGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQ 900
            D      HYALAVPLYTHFTSPLRRYPDIVVHR LAAA+EAEELY K     ++   DE 
Sbjct: 841  DSVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQ----KQTAIDEG 900

Query: 901  MRCFTGIHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACD 960
              CFTGIHF+KDAA+S+EG+EALS AAL+HGVP T++LSDVA +CN RKLA+R V DACD
Sbjct: 901  RSCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACD 960

Query: 961  KLYMWALLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTL 1020
            KLY W +LK+KEI   +ARV+ LG RFM++YI KL IERRIYYD++EGL  +WL+ATSTL
Sbjct: 961  KLYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTL 1020

Query: 1021 VLSFFGTRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGASTGGAAVEQ 1080
            ++    ++R  RG     +K +++   ++SPC++ V + +               A    
Sbjct: 1021 IVDKLYSKRGGRG----FFKPMKEAVYLVSPCEVCVAKCS---------------ALSVH 1048

Query: 1081 ESNLKSHISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            ++     +S   + PAVFPLT++L S+IPV LHAVGGDDGP+DIG R
Sbjct: 1081 DTESPEAVSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGAR 1048

BLAST of Sgr011635 vs. ExPASy Swiss-Prot
Match: Q0WPN0 (Inactive exonuclease DIS3L2 OS=Arabidopsis thaliana OX=3702 GN=SOV PE=2 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.1e-286
Identity = 564/1127 (50.04%), Postives = 737/1127 (65.39%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKK-RRSNRRSKQNASITTSVSCSSVNAIPGEASECMENG 60
            M+ A  + + ER ++G K+K+ + ++ NRRSKQ          SSV        E ++ G
Sbjct: 1    MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQ----------SSVPIEDAHVEESLD-G 60

Query: 61   RIDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFIN 120
            R  +   A  + SSS QQ     + +E    R + +A +S+PP+  +E G    S S + 
Sbjct: 61   RDSSRSKAKDSTSSSKQQR---PNTDELEAMRASNVAFNSMPPMR-AESGYPRRSASPL- 120

Query: 121  QHLHSSDAGGKFI-KSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVN 180
              L S +   + + KSCP   AC + PG+    +     + E + QRK F+S+W +D V 
Sbjct: 121  --LSSPEVSKQLLSKSCPDPRACEQSPGM----NGELFQQIEGSSQRKIFSSHWSLDAVT 180

Query: 181  EGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTS 240
            E L+KG+ FKA FRVNAHNR EAYCKIDG+P D+LING   Q+RAVEGD V IK+DP + 
Sbjct: 181  EALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKLDPLSL 240

Query: 241  WTRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVD-VDVKSDSFRSSSLPDKRC 300
            W +MKG      +    E  N      EKD    + KN +D V+   D F          
Sbjct: 241  WPKMKGFVT--ESAAKPEGTN---SPPEKDDKKARQKNGIDVVEGFEDGF---------- 300

Query: 301  CSDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLY 360
             S +   + G    + + P+           LDS    +   + + S A+ ++C +++ +
Sbjct: 301  -SKNKSSVIGKGAKNGVTPS-------SPPSLDSCLGSFCEQKGNCS-AVDKLCGILSSF 360

Query: 361  PSKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLS--SSSNQGYVQ 420
            P KRP G+VVA++EKS  R++IVG L+VK +     I+ KE + K C S  S S+  YVQ
Sbjct: 361  PHKRPTGQVVAVVEKSLVRDSIVGLLDVKGW-----IHYKESDPKRCKSPLSLSDDEYVQ 420

Query: 421  LMPNDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVL 480
            LMP D RFP ++V    LP  I+ RL+N D  +E+ELVAA+I +W E S  P A + H+ 
Sbjct: 421  LMPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLF 480

Query: 481  GRGSGVESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDP 540
            GRGS +E  I+AIL++N++   +FS  SL+ LP  PW++P+EE+Q R+DLR+LC+ TIDP
Sbjct: 481  GRGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDP 540

Query: 541  SSASDLDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPML 600
            S+A+DLDDALSV+ L  G FRVG+HIADVS+FVLP+TALD EA+ RSTSVYL+QRKI ML
Sbjct: 541  STATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISML 600

Query: 601  PPLLSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGL 660
            PPLLSEN+GSL+PG DRLAFS+  D+N  GDV + WIGRT+I SCCKLSY+HAQDIIDG 
Sbjct: 601  PPLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDGK 660

Query: 661  IDSASSKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLY 720
             D A      N  P LHG F W  V  SVK L EIS TL++KRFR+GAL+LENSK V+L+
Sbjct: 661  SDVAE-----NGWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLF 720

Query: 721  DEFGMPYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREF 780
            DE G+PYD      K SNFLVEEFMLLAN T AEVIS+ +  S+LLRRHPEP  RKL+EF
Sbjct: 721  DEHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYRASSLLRRHPEPNTRKLKEF 780

Query: 781  ELFCSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELK 840
            E FCS+HG  LD SSS Q Q SLE+I   L DD +  DIL +YA +PMQLA+YFC+G LK
Sbjct: 781  EGFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLK 840

Query: 841  DG-ENGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQ 900
            D      HYALAVPLYTHFTSPLRRYPDIVVHR LAAA+EAEELY K     ++   DE 
Sbjct: 841  DSVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQ----KQTAIDEG 900

Query: 901  MRCFTGIHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACD 960
              CFTGIHF+KDAA+S+EG+EALS AAL+HGVP T++LSDVA +CN RKLA+R V DACD
Sbjct: 901  RSCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACD 960

Query: 961  KLYMWALLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTL 1020
            KLY W +LK+KEI   +ARV+ LG RFM++YI KL IERRIYYD++EGL  +WL+ATSTL
Sbjct: 961  KLYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTL 1020

Query: 1021 VLSFFGTRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGASTGGAAVEQ 1080
            ++    ++R  RG     +K +++   ++SPC++ V + +               A    
Sbjct: 1021 IVDKLYSKRGGRG----FFKPMKEAVYLVSPCEVCVAKCS---------------ALSVH 1048

Query: 1081 ESNLKSHISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            ++     +S   + PAVFPLT++L S+IPV LHAVGGDDGP+DIG R
Sbjct: 1081 DTESPEAVSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGAR 1048

BLAST of Sgr011635 vs. ExPASy Swiss-Prot
Match: Q8IYB7 (DIS3-like exonuclease 2 OS=Homo sapiens OX=9606 GN=DIS3L2 PE=1 SV=4)

HSP 1 Score: 424.9 bits (1091), Expect = 3.8e-117
Identity = 295/844 (34.95%), Postives = 422/844 (50.00%), Query Frame = 0

Query: 164 QRKYFTSYWFMDDVNEGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRA 223
           ++  F +Y   +DV+EGL++G + +   R+N     EA+        D+ I+G+ ++NRA
Sbjct: 46  KKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRA 105

Query: 224 VEGDIVAIKVDPFTSW------TRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNK 283
           + GD+V +K+ P   W      +  K T  A+ +  P E    H  +      S K  N 
Sbjct: 106 LNGDLVVVKLLPEEHWKVVKPESNDKETEAAYESDIPEELCGHHLPQ-----QSLKSYND 165

Query: 284 VDVDVKSDSFRSSSLPDKRCCSDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYS 343
               +    F  S   D    +  N+++DG               V + SV  S +    
Sbjct: 166 SPDVIVEAQFDGSDSEDGHGIT-QNVLVDG---------------VKKLSVCVSEKGRED 225

Query: 344 SNQDDVSKAIGRICAVINLYPSKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIK 403
            +            A +    +   +    A+ EKS QR A V ++  KK       ++K
Sbjct: 226 GD------------APVTKDETTCISQDTRALSEKSLQRSAKVVYILEKKHSRAATGFLK 285

Query: 404 EMNKKSCLSSSSNQGYVQLMPNDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARI 463
            +  K   +S   + Y    P+D R P + V   D P     R  +      + L   RI
Sbjct: 286 LLADK---NSELFRKYALFSPSDHRVPRIYVPLKDCPQDFVARPKD----YANTLFICRI 345

Query: 464 HEWVEESSAPQAHVLHVLGRGSGVESHIDAILFENAIRTCEFSHDSLSCLPH-TPWKIPQ 523
            +W E+ +     +   LG+   +E   + IL E  +   +FS + L CLP   PW IP 
Sbjct: 346 VDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQGLPWTIPP 405

Query: 524 EELQYRRDLRNLCIFTIDPSSASDLDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDK 583
           EE   RRDLR  CIFTIDPS+A DLDDALS + LA+G F+VG+HIADVS+FV   + LDK
Sbjct: 406 EEFSKRRDLRKDCIFTIDPSTARDLDDALSCKPLADGNFKVGVHIADVSYFVPEGSDLDK 465

Query: 584 EAQIRSTSVYLLQRKIPMLPPLLSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTV 643
            A  R+TSVYL+Q+ +PMLP LL E + SLNP  D+L FS+   +   G + + W GRT+
Sbjct: 466 VAAERATSVYLVQKVVPMLPRLLCEELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRTI 525

Query: 644 ICSCCKLSYEHAQDIIDGLIDSASSKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKE 703
           I SC KLSYEHAQ     +I+S + KI     P +  + +   V  +V  L+ I+K L++
Sbjct: 526 IRSCTKLSYEHAQ----SMIESPTEKIPAKELPPISPEHSSEEVHQAVLNLHGIAKQLRQ 585

Query: 704 KRFRDGALRLENSKRVYLYD-EFGMPYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTF 763
           +RF DGALRL+  K  +  D E G+P     YE+++SN LVEEFMLLAN  VA  I R F
Sbjct: 586 QRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRESNKLVEEFMLLANMAVAHKIHRAF 645

Query: 764 PDSALLRRHPEPIMRKLREFELFCSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLF--- 823
           P+ ALLRRHP P  R L +   FC + G  +D SS+    +SL Q      DD       
Sbjct: 646 PEQALLRRHPPPQTRMLSDLVEFCDQMGLPVDFSSAGALNKSLTQ---TFGDDKYSLARK 705

Query: 824 DILISYATRPMQLATYFCSGELKDGENGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAA 883
           ++L +  +RPMQ+A YFCSG L+D     HYAL VPLYTHFTSP+RR+ D++VHR LAAA
Sbjct: 706 EVLTNMCSRPMQMALYFCSGLLQDPAQFRHYALNVPLYTHFTSPIRRFADVLVHRLLAAA 765

Query: 884 IEAEELYLKHQGIIQKVNGDEQMRCFTGIHFDKDAADSLEGREALSSAALRHGVPCTKLL 943
                                                 L  RE L  A      P T  L
Sbjct: 766 --------------------------------------LGYRERLDMA------PDT--L 796

Query: 944 SDVALHCNNRKLASRHVADACDKLYMWALLKKKEILFSDARVLGLGPRFMSLYIQKLAIE 997
              A HCN+R++AS+ V +    L+   L+K+   L S+A V+G+  +   + + +  ++
Sbjct: 826 QKQADHCNDRRMASKRVQELSTSLFFAVLVKESGPLESEAMVMGILKQAFDVLVLRYGVQ 796

BLAST of Sgr011635 vs. ExPASy Swiss-Prot
Match: Q8CI75 (DIS3-like exonuclease 2 OS=Mus musculus OX=10090 GN=Dis3l2 PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 4.7e-115
Identity = 283/864 (32.75%), Postives = 426/864 (49.31%), Query Frame = 0

Query: 140 ACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVNEGLQKGDIFKAFFRVNAHNRL 199
           A G  PG   ++     +++    ++  F +Y   +DV+EGL++G + +   R+N     
Sbjct: 27  AVGASPGDKKSK-----NKSMRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFH 86

Query: 200 EAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTSWTRMKGTTEAHNNMHPMEDAN 259
           EA+        D+ I+G+ ++NRA+ GD+V +K+ P   W  +K                
Sbjct: 87  EAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEDQWKAVK---------------- 146

Query: 260 LHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKR-CCSDDNIVLDGTACDDDLLPNY 319
              E N+K+  +       + D+  +      L   R   S  +++++    D D    +
Sbjct: 147 --PESNDKEIEA-----TYEADIPEEGCGHHPLQQSRKGWSGPDVIIEAQFDDSDSEDRH 206

Query: 320 EQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYPSKRPAGR-VVAILEKSRQRE 379
                    V   S +     ++D S  +        +     P  +    + EKS Q+ 
Sbjct: 207 GNTSGLVDGVKKLSISTPDRGKEDSSTPV--------MKDENTPIPQDTRGLSEKSLQKS 266

Query: 380 AIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMPNDARFPLMMVLAGDLPNCI 439
           A V ++  KK        +K +  K   +S   + Y    P+D R P + V   D P   
Sbjct: 267 AKVVYILEKKHSRAATGILKLLADK---NSDLFKKYALFSPSDHRVPRIYVPLKDCPQDF 326

Query: 440 KKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRGSGVESHIDAILFENAIRTC 499
             R  +      + L   RI +W E+ +     +   LG+   +E   + IL E  +   
Sbjct: 327 MTRPKD----FANTLFICRIIDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFS 386

Query: 500 EFSHDSLSCLPHT-PWKIPQEELQYRRDLRNLCIFTIDPSSASDLDDALSVEKLANGIFR 559
           +FS + L CLP + PW IP +E+  RRDLR  CIFTIDPS+A DLDDAL+  +L +G F 
Sbjct: 387 DFSSEVLECLPQSLPWTIPPDEVGKRRDLRKDCIFTIDPSTARDLDDALACRRLTDGTFE 446

Query: 560 VGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSENIGSLNPGVDRLAFS 619
           VG+HIADVS+FV   ++LDK A  R+TSVYL+Q+ +PMLP LL E + SLNP  D+L FS
Sbjct: 447 VGVHIADVSYFVPEGSSLDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMTDKLTFS 506

Query: 620 LFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDSASSKILGNHCPQLHGQFA 679
           +   +   G +   W GRT+I SC KLSY+HAQ     +I++ + KI     P +  + +
Sbjct: 507 VIWKLTPEGKILEEWFGRTIIRSCTKLSYDHAQ----SMIENPTEKIPEEELPPISPEHS 566

Query: 680 WPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYD-EFGMPYDSTFYEHKDSNFL 739
              V  +V  L+ I+K L+ +RF DGALRL+  K  +  D E G+P     YE++DSN L
Sbjct: 567 VEEVHQAVLNLHSIAKQLRRQRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRDSNKL 626

Query: 740 VEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELFCSRHGFVLDTSSSVQFQ 799
           VEEFMLLAN  VA  I RTFP+ ALLRRHP P  + L +   FC + G  +D SS+    
Sbjct: 627 VEEFMLLANMAVAHKIFRTFPEQALLRRHPPPQTKMLSDLVEFCDQMGLPMDVSSAGALN 686

Query: 800 RSLEQIRLKLHDDPLLF---DILISYATRPMQLATYFCSGELKDGENGSHYALAVPLYTH 859
           +SL +      DD       ++L +  +RPMQ+A YFCSG L+D E   HYAL VPLYTH
Sbjct: 687 KSLTK---TFGDDKYSLARKEVLTNMYSRPMQMALYFCSGMLQDQEQFRHYALNVPLYTH 746

Query: 860 FTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCFTGIHFDKDAADSLE 919
           FTSP+RR+ D++VHR LAAA+   E        +QK                        
Sbjct: 747 FTSPIRRFADVIVHRLLAAALGYSEQPDVEPDTLQK------------------------ 794

Query: 920 GREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYMWALLKKKEILFSDA 979
                                  A HCN+R++AS+ V +    L+   L+K+   L S+A
Sbjct: 807 ----------------------QADHCNDRRMASKRVQELSIGLFFAVLVKESGPLESEA 794

Query: 980 RVLGLGPRFMSLYIQKLAIERRIY 997
            V+G+  +   + + +  +++RIY
Sbjct: 867 MVMGVLNQAFDVLVLRFGVQKRIY 794

BLAST of Sgr011635 vs. ExPASy Swiss-Prot
Match: Q0V9R3 (DIS3-like exonuclease 2 OS=Xenopus tropicalis OX=8364 GN=dis3l2 PE=2 SV=2)

HSP 1 Score: 415.2 bits (1066), Expect = 3.0e-114
Identity = 282/835 (33.77%), Postives = 407/835 (48.74%), Query Frame = 0

Query: 164 QRKYFTSYWFMDDVNEGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRA 223
           ++  F +Y   ++V+ GL++G++ +   R+N     EAY        D+ I+G+  +NRA
Sbjct: 33  KKSVFEAYMTKEEVSAGLKRGELIQGPLRINPKKFHEAYLPSPDGVRDLFIDGVVPRNRA 92

Query: 224 VEGDIVAIKVDPFTSWTRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVK 283
           + GD+V +K+ P   W  +K               N   E+++  GHS   K        
Sbjct: 93  LNGDVVVVKLLPQEQWKVLK---------------NDVCEDDDTPGHSTGNKQ----HAL 152

Query: 284 SDSFRSSSLPDKRCCSDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDV 343
           S     SS  +     +  +        +  L    Q ++     L + +   S   D  
Sbjct: 153 SPHLMKSSAKNPDLIIEAKVDSSAEDGHESALIGCLQKEIKDQDKLGAIEEKTSKQGD-- 212

Query: 344 SKAIGRICAVINLYPSKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKS 403
            K     C         +   +VV ILEK   R A                +IK ++ K 
Sbjct: 213 PKTFSDDCF--------QKTAKVVYILEKKHSRAATG--------------FIKPLSDK- 272

Query: 404 CLSSSSNQGYVQLMPNDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEE 463
             SS   +      P D R P + V  GD P+      +    T  + L    I  W ++
Sbjct: 273 --SSDLARKRALFSPVDHRLPRIYVPLGDCPHDFAIHPE----TYANTLFICSITAWRDD 332

Query: 464 SSAPQAHVLHVLGRGSGVESHIDAILFENAIRTCEFSHDSLSCLPH-TPWKIPQEELQYR 523
           S+  +  ++  LG+   +E   + IL E  +   +F    L CLP   PW IPQEE Q R
Sbjct: 333 SNFAEGKLMKSLGQAGEIEPETEGILVEYGVDFSDFPDKVLQCLPQDLPWTIPQEEFQKR 392

Query: 524 RDLRNLCIFTIDPSSASDLDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRS 583
           +DLRN CIFTIDP++A DLDDALS + L +G F VG+HIADVS+FV   +ALD  A  R+
Sbjct: 393 KDLRNECIFTIDPATARDLDDALSCKPLPDGNFEVGVHIADVSYFVAEGSALDIMASERA 452

Query: 584 TSVYLLQRKIPMLPPLLSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCK 643
           TSVYL+Q+ IPMLP LL E + SLNP  DRL FS+   I   G++ + W GR+VICSC K
Sbjct: 453 TSVYLVQKVIPMLPRLLCEELCSLNPMTDRLTFSVIWKITPQGEILDEWFGRSVICSCVK 512

Query: 644 LSYEHAQDIIDGLIDSASSKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDG 703
           LSY+HAQ+    +I+    KI  +  P +  Q     +  +V  L+ I++ L+++RF DG
Sbjct: 513 LSYDHAQN----MINHPDKKIEQHELPPVSPQHTINEIHQAVLNLHLIAQNLRKQRFDDG 572

Query: 704 ALRLENSKRVYLYD-EFGMPYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALL 763
           ALRL+  K  +  D E G+P     Y+++DSN LVEEFMLLAN  VA  I R FP+ ALL
Sbjct: 573 ALRLDQLKLTFTLDKESGLPQGCYIYQYRDSNKLVEEFMLLANMAVAHHIYRRFPEEALL 632

Query: 764 RRHPEPIMRKLREFELFCSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATR 823
           RRHP P  + L +   FC + G  LD SSS    +SL              ++L +  +R
Sbjct: 633 RRHPPPQTKMLNDLIEFCDQMGLQLDFSSSGTLHKSLNDQFETDEYSAARKEVLTNMCSR 692

Query: 824 PMQLATYFCSGELKDGENGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLK 883
           PMQ+A YFC+G LKD     HYAL VPLYTHFTSP+RR+ D++VHR LAA++        
Sbjct: 693 PMQMAVYFCTGALKDETLFHHYALNVPLYTHFTSPIRRFADVIVHRLLAASLGCGPPLKM 752

Query: 884 HQGIIQKVNGDEQMRCFTGIHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNN 943
            + +IQK                                               A HCN+
Sbjct: 753 PKEVIQK----------------------------------------------QADHCND 767

Query: 944 RKLASRHVADACDKLYMWALLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIY 997
           RK AS+ V +   +L+    +K+   L S+A V+G+      + + +  +++RIY
Sbjct: 813 RKTASKRVQELSAELFFSVFVKECGPLESEAMVMGVLNEAFDVIVLRFGVQKRIY 767

BLAST of Sgr011635 vs. ExPASy TrEMBL
Match: A0A6J1CKE7 (DIS3-like exonuclease 2 OS=Momordica charantia OX=3673 GN=LOC111011815 PE=3 SV=1)

HSP 1 Score: 1968.0 bits (5097), Expect = 0.0e+00
Identity = 987/1124 (87.81%), Postives = 1042/1124 (92.70%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKKRRSNRRSK--QNASITTSVSCSSVNAIPGEASECMEN 60
            MR AVEQ T ERN+DGEKEK+KKRRSNRRSK  Q ASITT+VSCSSVN IPGE SECMEN
Sbjct: 1    MRAAVEQSTSERNEDGEKEKRKKRRSNRRSKQTQTASITTAVSCSSVNEIPGETSECMEN 60

Query: 61   GRIDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFI 120
            GRID NLTA +NYSS  +  Y SN+P EHGLTRTNKIA SSLPPLHISEQ +  ESQ+ I
Sbjct: 61   GRIDANLTAPTNYSSLTEPAYRSNNPTEHGLTRTNKIAFSSLPPLHISEQAKFSESQNLI 120

Query: 121  NQHLHSSDAGGKFIKSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVN 180
            NQH HSS+AGG+ IKSCPQ+IA GR+PGIS NQ+S PAH TENN QRKYFTS+W MDDVN
Sbjct: 121  NQHFHSSNAGGRIIKSCPQEIASGRVPGISANQNSLPAHVTENNSQRKYFTSHWSMDDVN 180

Query: 181  EGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTS 240
            EGLQKGDIF A FRVNAHN LEAYCKIDGLPVDVLINGIASQNRAVEGD VAIKVDPFTS
Sbjct: 181  EGLQKGDIFIALFRVNAHNGLEAYCKIDGLPVDVLINGIASQNRAVEGDTVAIKVDPFTS 240

Query: 241  WTRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKRCC 300
            WTRMKG +EAHNNMH MEDAN+HAEEN KDGH+C+ KNKVDV VKS++FRSSSLPDKRCC
Sbjct: 241  WTRMKGASEAHNNMHSMEDANIHAEENGKDGHNCEEKNKVDV-VKSNNFRSSSLPDKRCC 300

Query: 301  SDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYP 360
            S++N VLDGTAC D LL NYEQ DVYQS VLDSSQAHYS NQDDVSKAIG+ICAVINL+P
Sbjct: 301  SEENKVLDGTAC-DVLLSNYEQSDVYQSLVLDSSQAHYSCNQDDVSKAIGKICAVINLHP 360

Query: 361  SKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMP 420
            SKRP GRVVAILE S QRE+IVGHL VKKFLSFQEIY+KEMN KSCL SS N GYVQLMP
Sbjct: 361  SKRPTGRVVAILENSLQRESIVGHLIVKKFLSFQEIYMKEMNTKSCLPSSPNHGYVQLMP 420

Query: 421  NDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRG 480
            NDARFP+MMVL  DLP+ IKKRLDNGDVTVESELVAARIHEWV+ESSAP+AHVLHVLG+G
Sbjct: 421  NDARFPMMMVLTEDLPDHIKKRLDNGDVTVESELVAARIHEWVQESSAPRAHVLHVLGQG 480

Query: 481  SGVESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDPSSA 540
            S VESH+DAILF+NAIRTCEFSHDSLSCLPHTPWKIP +ELQ RRDLRNLCIFTIDPS+A
Sbjct: 481  SEVESHVDAILFQNAIRTCEFSHDSLSCLPHTPWKIPPDELQCRRDLRNLCIFTIDPSTA 540

Query: 541  SDLDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPL 600
            SDLDDALSVEKLANGIFRVGIHIADVSHFVLP+TALDKEAQIRST  YLLQRKIPMLPPL
Sbjct: 541  SDLDDALSVEKLANGIFRVGIHIADVSHFVLPETALDKEAQIRSTCFYLLQRKIPMLPPL 600

Query: 601  LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDS 660
            LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGL+DS
Sbjct: 601  LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLLDS 660

Query: 661  ASSKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYDEF 720
              SKI  NHCP LHGQFAWP VISSVKIL+EISKTLKEKRFRDGALRL+NSKRVYLYDE+
Sbjct: 661  DCSKISRNHCPHLHGQFAWPDVISSVKILHEISKTLKEKRFRDGALRLDNSKRVYLYDEY 720

Query: 721  GMPYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELF 780
            G+PYDS FYEHKDSNFLVEEFMLLANTTVAEV+SRTFPDSALLRRHPEP+MRKLREFE F
Sbjct: 721  GIPYDSKFYEHKDSNFLVEEFMLLANTTVAEVVSRTFPDSALLRRHPEPVMRKLREFESF 780

Query: 781  CSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKDGE 840
            CS+HGF LDTSSSVQFQ+SLEQIR KLHDDPLLFDILISYATRPMQLATYFCSGELKDGE
Sbjct: 781  CSKHGFELDTSSSVQFQQSLEQIRKKLHDDPLLFDILISYATRPMQLATYFCSGELKDGE 840

Query: 841  NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF 900
            NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF
Sbjct: 841  NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF 900

Query: 901  TGIHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYM 960
            TGIHFDKDAADSLEGREALS+AAL+HGVPCTKLLSDVALHCNNRKLAS+HVADACDKLYM
Sbjct: 901  TGIHFDKDAADSLEGREALSAAALKHGVPCTKLLSDVALHCNNRKLASKHVADACDKLYM 960

Query: 961  WALLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTLVLSF 1020
            WALLKKKEIL SDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDA+STLVLSF
Sbjct: 961  WALLKKKEILLSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDASSTLVLSF 1020

Query: 1021 FGTRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGAS-TGGAAVEQESN 1080
            FGTRRS++ RGSSKWKALE+VALVISPCDLNVQ+R LGGSPSE GG S T GA VEQESN
Sbjct: 1021 FGTRRSFKSRGSSKWKALEEVALVISPCDLNVQERILGGSPSESGGXSTTEGATVEQESN 1080

Query: 1081 LKSHISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            LKSH SDTGI PAVFPLTVRL S+IPVALHA+GGDDGPIDIGVR
Sbjct: 1081 LKSHTSDTGIVPAVFPLTVRLFSTIPVALHAIGGDDGPIDIGVR 1122

BLAST of Sgr011635 vs. ExPASy TrEMBL
Match: A0A6J1CHZ9 (DIS3-like exonuclease 2 OS=Momordica charantia OX=3673 GN=LOC111011815 PE=3 SV=1)

HSP 1 Score: 1968.0 bits (5097), Expect = 0.0e+00
Identity = 987/1124 (87.81%), Postives = 1042/1124 (92.70%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKKRRSNRRSK--QNASITTSVSCSSVNAIPGEASECMEN 60
            MR AVEQ T ERN+DGEKEK+KKRRSNRRSK  Q ASITT+VSCSSVN IPGE SECMEN
Sbjct: 1    MRAAVEQSTSERNEDGEKEKRKKRRSNRRSKQTQTASITTAVSCSSVNEIPGETSECMEN 60

Query: 61   GRIDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFI 120
            GRID NLTA +NYSS  +  Y SN+P EHGLTRTNKIA SSLPPLHISEQ +  ESQ+ I
Sbjct: 61   GRIDANLTAPTNYSSLTEPAYRSNNPTEHGLTRTNKIAFSSLPPLHISEQAKFSESQNLI 120

Query: 121  NQHLHSSDAGGKFIKSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVN 180
            NQH HSS+AGG+ IKSCPQ+IA GR+PGIS NQ+S PAH TENN QRKYFTS+W MDDVN
Sbjct: 121  NQHFHSSNAGGRIIKSCPQEIASGRVPGISANQNSLPAHVTENNSQRKYFTSHWSMDDVN 180

Query: 181  EGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTS 240
            EGLQKGDIF A FRVNAHN LEAYCKIDGLPVDVLINGIASQNRAVEGD VAIKVDPFTS
Sbjct: 181  EGLQKGDIFIALFRVNAHNGLEAYCKIDGLPVDVLINGIASQNRAVEGDTVAIKVDPFTS 240

Query: 241  WTRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKRCC 300
            WTRMKG +EAHNNMH MEDAN+HAEEN KDGH+C+ KNKVDV VKS++FRSSSLPDKRCC
Sbjct: 241  WTRMKGASEAHNNMHSMEDANIHAEENGKDGHNCEEKNKVDV-VKSNNFRSSSLPDKRCC 300

Query: 301  SDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYP 360
            S++N VLDGTAC D LL NYEQ DVYQS VLDSSQAHYS NQDDVSKAIG+ICAVINL+P
Sbjct: 301  SEENKVLDGTAC-DVLLSNYEQSDVYQSLVLDSSQAHYSCNQDDVSKAIGKICAVINLHP 360

Query: 361  SKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMP 420
            SKRP GRVVAILE S QRE+IVGHL VKKFLSFQEIY+KEMN KSCL SS N GYVQLMP
Sbjct: 361  SKRPTGRVVAILENSLQRESIVGHLIVKKFLSFQEIYMKEMNTKSCLPSSPNHGYVQLMP 420

Query: 421  NDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRG 480
            NDARFP+MMVL  DLP+ IKKRLDNGDVTVESELVAARIHEWV+ESSAP+AHVLHVLG+G
Sbjct: 421  NDARFPMMMVLTEDLPDHIKKRLDNGDVTVESELVAARIHEWVQESSAPRAHVLHVLGQG 480

Query: 481  SGVESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDPSSA 540
            S VESH+DAILF+NAIRTCEFSHDSLSCLPHTPWKIP +ELQ RRDLRNLCIFTIDPS+A
Sbjct: 481  SEVESHVDAILFQNAIRTCEFSHDSLSCLPHTPWKIPPDELQCRRDLRNLCIFTIDPSTA 540

Query: 541  SDLDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPL 600
            SDLDDALSVEKLANGIFRVGIHIADVSHFVLP+TALDKEAQIRST  YLLQRKIPMLPPL
Sbjct: 541  SDLDDALSVEKLANGIFRVGIHIADVSHFVLPETALDKEAQIRSTCFYLLQRKIPMLPPL 600

Query: 601  LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDS 660
            LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGL+DS
Sbjct: 601  LSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLLDS 660

Query: 661  ASSKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYDEF 720
              SKI  NHCP LHGQFAWP VISSVKIL+EISKTLKEKRFRDGALRL+NSKRVYLYDE+
Sbjct: 661  DCSKISRNHCPHLHGQFAWPDVISSVKILHEISKTLKEKRFRDGALRLDNSKRVYLYDEY 720

Query: 721  GMPYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELF 780
            G+PYDS FYEHKDSNFLVEEFMLLANTTVAEV+SRTFPDSALLRRHPEP+MRKLREFE F
Sbjct: 721  GIPYDSKFYEHKDSNFLVEEFMLLANTTVAEVVSRTFPDSALLRRHPEPVMRKLREFESF 780

Query: 781  CSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKDGE 840
            CS+HGF LDTSSSVQFQ+SLEQIR KLHDDPLLFDILISYATRPMQLATYFCSGELKDGE
Sbjct: 781  CSKHGFELDTSSSVQFQQSLEQIRKKLHDDPLLFDILISYATRPMQLATYFCSGELKDGE 840

Query: 841  NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF 900
            NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF
Sbjct: 841  NGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCF 900

Query: 901  TGIHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYM 960
            TGIHFDKDAADSLEGREALS+AAL+HGVPCTKLLSDVALHCNNRKLAS+HVADACDKLYM
Sbjct: 901  TGIHFDKDAADSLEGREALSAAALKHGVPCTKLLSDVALHCNNRKLASKHVADACDKLYM 960

Query: 961  WALLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTLVLSF 1020
            WALLKKKEIL SDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDA+STLVLSF
Sbjct: 961  WALLKKKEILLSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDASSTLVLSF 1020

Query: 1021 FGTRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGAS-TGGAAVEQESN 1080
            FGTRRS++ RGSSKWKALE+VALVISPCDLNVQ+R LGGSPSE GG S T GA VEQESN
Sbjct: 1021 FGTRRSFKSRGSSKWKALEEVALVISPCDLNVQERILGGSPSESGGXSTTEGATVEQESN 1080

Query: 1081 LKSHISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            LKSH SDTGI PAVFPLTVRL S+IPVALHA+GGDDGPIDIGVR
Sbjct: 1081 LKSHTSDTGIVPAVFPLTVRLFSTIPVALHAIGGDDGPIDIGVR 1122

BLAST of Sgr011635 vs. ExPASy TrEMBL
Match: A0A6J1JX50 (DIS3-like exonuclease 2 OS=Cucurbita maxima OX=3661 GN=LOC111489125 PE=3 SV=1)

HSP 1 Score: 1922.5 bits (4979), Expect = 0.0e+00
Identity = 961/1121 (85.73%), Postives = 1029/1121 (91.79%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKKRRSNRRSKQNASITTSVSCSSVNAIPGEASECMENGR 60
            MRGAVEQ TPER DDG+KEKKKKRRSNRRSKQNASI+TSVSCSSVN + GEASECMENG+
Sbjct: 1    MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60

Query: 61   IDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFINQ 120
            ID NLTA SN+SS  QQ + SNHP EHG+TR NKIA SSLPPLHISEQ EL ESQ+ IN+
Sbjct: 61   IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120

Query: 121  HLHSSDAGGKFIKSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVNEG 180
            +LH  DAGGK IKSCP+QI CGRMPGIS NQHS PA  TENN QRKYF S+W ++DV+EG
Sbjct: 121  NLHPLDAGGKTIKSCPEQIVCGRMPGISTNQHSSPADVTENNSQRKYFASHWSVEDVDEG 180

Query: 181  LQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTSWT 240
            LQKGDIF+AFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAI VDP TSWT
Sbjct: 181  LQKGDIFRAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTSWT 240

Query: 241  RMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKRCCSD 300
            RMKGT+E HN+ H MEDANL AE  E DG +CKGKNKVD  VKSDSFRSSS PDKRCCS+
Sbjct: 241  RMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKVDASVKSDSFRSSSSPDKRCCSE 300

Query: 301  DNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYPSK 360
            D I LDGTACDD LL N EQ DVYQS V+D  +AHYSSNQDDVSKAI RICAVI+ YP K
Sbjct: 301  DKI-LDGTACDDLLLKN-EQRDVYQSLVVDLPEAHYSSNQDDVSKAIQRICAVISSYPGK 360

Query: 361  RPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMPND 420
            RP GRVVAILEKSRQRE+IVGHLNVKKFLSFQEIY+KEMN KSCLS S N GYVQLMPND
Sbjct: 361  RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420

Query: 421  ARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRGSG 480
            ARFP+M+VLAGDLP+ IKKRLDNGDVTVE+ELVA +IHEWV+ESSAPQAHVLHVLGRGS 
Sbjct: 421  ARFPIMVVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQAHVLHVLGRGSE 480

Query: 481  VESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDPSSASD 540
            V SHIDAILFENAI +CEFS+DSL+CLPHTPWKIP EELQ RRDLRNLCIFTIDPSSASD
Sbjct: 481  VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540

Query: 541  LDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
            LDDALSV+KLANGIFRVG+HIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS
Sbjct: 541  LDDALSVQKLANGIFRVGVHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDSAS 660
            EN+GSL+PGVDRLAFSLFLDI++CGDVK+ WIGRTVICSCCKLSYEHAQDIIDGLIDS S
Sbjct: 601  ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660

Query: 661  SKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYDEFGM 720
            SK LGN+ PQLHGQF W  VISSVKIL+EISKTLK+KRFRDGALRLENSK VYLYDE+G+
Sbjct: 661  SKNLGNNYPQLHGQFEWLDVISSVKILHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720

Query: 721  PYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELFCS 780
            PYDS FYE KDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPI+RKLREFE FCS
Sbjct: 721  PYDSAFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780

Query: 781  RHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKDGENG 840
            +HGF LDTSSSVQFQ+SLEQIR+KLHDDPLLFDIL SYATRPMQLATYFCSGELKDGE G
Sbjct: 781  KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCFTG 900
            SHYALAVPLYTHFTSPLRRYPDI+VHRTLAAAIEAE+LYLKH+GI QKVN DEQ+RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIIVHRTLAAAIEAEKLYLKHRGITQKVNSDEQIRCFTG 900

Query: 901  IHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYMWA 960
            ++FDKDAADSLEG+EALSSAALRHGVPC KLL+DVALHCNNRKLAS+HVAD CDKLYMWA
Sbjct: 901  MYFDKDAADSLEGKEALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960

Query: 961  LLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTLVLSFFG 1020
            LLKKK++LFSDARVLGLGPRFMSLYIQKLAIERRIYYDE EGLAVEWL+ TSTLVLSFFG
Sbjct: 961  LLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020

Query: 1021 TRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGASTGGAAVEQESNLKS 1080
            TRRS+R RGS KWKALEDVALV+SPCD NV+QR LG SPSELGG  TGGA VEQESNLKS
Sbjct: 1021 TRRSHRSRGSIKWKALEDVALVVSPCDQNVKQRALGASPSELGGTGTGGAVVEQESNLKS 1080

Query: 1081 HISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            H+SDTGIDPAVFPLTVRLLS++PVALHAVGGDDGPIDIGVR
Sbjct: 1081 HVSDTGIDPAVFPLTVRLLSTLPVALHAVGGDDGPIDIGVR 1119

BLAST of Sgr011635 vs. ExPASy TrEMBL
Match: A0A6J1FJM2 (DIS3-like exonuclease 2 OS=Cucurbita moschata OX=3662 GN=LOC111444646 PE=3 SV=1)

HSP 1 Score: 1919.1 bits (4970), Expect = 0.0e+00
Identity = 960/1121 (85.64%), Postives = 1032/1121 (92.06%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKKRRSNRRSKQNASITTSVSCSSVNAIPGEASECMENGR 60
            MRGAVEQ TPER DDG+KEKKKKRRSNRRSKQNASI+TSVSCSSVN +PGEASEC ENG+
Sbjct: 1    MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMPGEASECRENGK 60

Query: 61   IDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFINQ 120
            I+ NLTA SN+SS  QQ + SNHP EHG+TR NKIA SSLPPLHISEQ EL ESQ+ IN+
Sbjct: 61   INANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120

Query: 121  HLHSSDAGGKFIKSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVNEG 180
            +LH  DAGGK IKSCP+QI CGRMPGIS+NQHSPPA  TENN QRKYF S+W ++DV+EG
Sbjct: 121  NLHPLDAGGKTIKSCPEQIVCGRMPGISINQHSPPADVTENNTQRKYFASHWSVEDVDEG 180

Query: 181  LQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTSWT 240
            LQKGDIF+AFFRVN+HNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAI VDP TSWT
Sbjct: 181  LQKGDIFRAFFRVNSHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTSWT 240

Query: 241  RMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKRCCSD 300
            RMKGT+E HN+ H MEDANL AE  E DG +CKGKNK+D  VKSDSFRSSS PDKRCCS+
Sbjct: 241  RMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKLDASVKSDSFRSSSSPDKRCCSE 300

Query: 301  DNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYPSK 360
            D I LDGTACDD LL N EQ DVYQSSV+D  +AHYS NQDDVSKAI RICAVI+ YP K
Sbjct: 301  DKI-LDGTACDDLLLKN-EQRDVYQSSVVDPPEAHYSRNQDDVSKAIQRICAVISSYPGK 360

Query: 361  RPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMPND 420
            RP GRVVAILEKSRQRE+IVGHLNVKKFLSFQEIY+KEMN KSCLS S N GYVQLMPND
Sbjct: 361  RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420

Query: 421  ARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRGSG 480
            ARFP+MMVLAGDLP+ IKKRLDNGDVTVE+ELVA +IHEWV+ESSAPQA VLHVLGRGS 
Sbjct: 421  ARFPIMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQALVLHVLGRGSE 480

Query: 481  VESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDPSSASD 540
            V SHIDAILFENAI +CEFS+DSL+CLPHTPWKIP EELQ RRDLRNLCIFTIDPSSASD
Sbjct: 481  VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540

Query: 541  LDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
            LDDALSV+KLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS
Sbjct: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDSAS 660
            +N+GSL+PGVDRLAFSLFLDI++ GDVK+ WIGRTVICSCCKLSYEHAQDIIDGLIDS +
Sbjct: 601  KNVGSLSPGVDRLAFSLFLDIDNSGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDN 660

Query: 661  SKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYDEFGM 720
             K LGN+ PQLHGQFAWP VISSVK+L+EISKTLK+KRFRDGALRLENSK VYLYDE+G+
Sbjct: 661  LKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720

Query: 721  PYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELFCS 780
            PYDSTFYE KDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPI+RKLREFE FCS
Sbjct: 721  PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780

Query: 781  RHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKDGENG 840
            +HGF LDTSSSVQFQ+SLEQIR+KLHDDPLLFDIL SYATRPMQLATYFCSGELKDGE G
Sbjct: 781  KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCFTG 900
            SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAE+LYLKH+GIIQKVN DEQ+RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGIIQKVNSDEQIRCFTG 900

Query: 901  IHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYMWA 960
            ++FDKDAADSLEGREALSSAALRHGVPC KLL+DVALHCN+RKLAS+HVAD CDKLYMWA
Sbjct: 901  MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNDRKLASKHVADGCDKLYMWA 960

Query: 961  LLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTLVLSFFG 1020
            +LKKK++LFSDARVLGLGPRFMSLYIQKLAIERRIYYDE EGLAVEWL+ TSTLVLSFFG
Sbjct: 961  VLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020

Query: 1021 TRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGASTGGAAVEQESNLKS 1080
            TRRS+R RGS KWKALEDVALV+SPCD NV+QR LG SPSELGG  TGGA VEQESNLKS
Sbjct: 1021 TRRSHRSRGSIKWKALEDVALVVSPCDHNVKQRALGVSPSELGGTGTGGAVVEQESNLKS 1080

Query: 1081 HISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            H+SDTGIDPAVFPLTVRLLS+IPVALHAVGGDDGPIDIGVR
Sbjct: 1081 HVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVR 1119

BLAST of Sgr011635 vs. ExPASy TrEMBL
Match: A0A5D3CLM1 (DIS3-like exonuclease 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00340 PE=3 SV=1)

HSP 1 Score: 1851.6 bits (4795), Expect = 0.0e+00
Identity = 934/1120 (83.39%), Postives = 1010/1120 (90.18%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKKRRSNRRSKQNASITTSVSCSSVNAIPGEASECMENGR 60
            MR A EQ TPERN D +KEKKKKRRSNRRSK N S+TTS S +SVN I GEASECMENGR
Sbjct: 1    MRAAFEQSTPERNGDCDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60

Query: 61   IDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFINQ 120
            ID NLT+ SNYSS  QQE  SNHP EHGLT  NKIA SSLP LHI++Q EL  SQ+ INQ
Sbjct: 61   IDANLTSPSNYSSLTQQENHSNHPIEHGLTGGNKIAFSSLPSLHINDQAELSASQNLINQ 120

Query: 121  HLHSSDAGGKFIKSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVNEG 180
            + HSSDAGG+ IKSCP+QIA GR  GIS NQ SPPA  TENN QRKYF S+W +DDVNEG
Sbjct: 121  NHHSSDAGGRIIKSCPEQIASGRNSGISSNQLSPPADLTENNTQRKYFPSHWSIDDVNEG 180

Query: 181  LQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTSWT 240
            LQKGDIFKA FRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFT WT
Sbjct: 181  LQKGDIFKALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTVWT 240

Query: 241  RMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVDVDVKSDSFRSSSLPDKRCCSD 300
            +MKGT+EAH+N+  M+DANL AE  EK+ H+CKGKNK D D KSDSFRSSSLPDKRCCS+
Sbjct: 241  KMKGTSEAHDNIKSMDDANLPAEPTEKNSHNCKGKNKFDADGKSDSFRSSSLPDKRCCSE 300

Query: 301  DNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLYPSK 360
            D  VLDG +C DDLL NYEQCD+ Q SV+D SQAH+SSNQ DVSK IGRICA+INLYP+K
Sbjct: 301  DK-VLDGISC-DDLLSNYEQCDINQLSVVDPSQAHHSSNQYDVSKIIGRICALINLYPAK 360

Query: 361  RPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLSSSSNQGYVQLMPND 420
            RP GRVV ILEKSR R+ +VGHLNVKKFLSFQE Y+KE N KSCLS S N GYVQLMPND
Sbjct: 361  RPTGRVVTILEKSRLRDNVVGHLNVKKFLSFQEFYVKE-NTKSCLSPSQNGGYVQLMPND 420

Query: 421  ARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVLGRGSG 480
            ARFP+MMVLAGDLP+CIKKRLDNGDVTVE+ELVAARI++WV+ESS+P+AHVLHVLGRGS 
Sbjct: 421  ARFPIMMVLAGDLPDCIKKRLDNGDVTVENELVAARIYDWVKESSSPRAHVLHVLGRGSE 480

Query: 481  VESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDPSSASD 540
            VESHIDAILFENAIRTCEFSHDSLSC+PHTPWKIP EEL+ RRD+RNLCIFTIDPSSASD
Sbjct: 481  VESHIDAILFENAIRTCEFSHDSLSCIPHTPWKIPHEELRCRRDIRNLCIFTIDPSSASD 540

Query: 541  LDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
            LDDALSV+KLAN IFRVGIHIADVS+FVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS
Sbjct: 541  LDDALSVQKLANDIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGLIDSAS 660
            ENIGSLNPGVDRLAFSLFLDIN CGDVK+ WI RTVIC CCKLSYE+AQDIIDGLIDS S
Sbjct: 601  ENIGSLNPGVDRLAFSLFLDINGCGDVKDYWIERTVICCCCKLSYEYAQDIIDGLIDSDS 660

Query: 661  SKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYDEFGM 720
             +I GN+CPQLHGQF W  VISSVK+L+EISKTLKEKRFRDGALRLENSK +YLYDE+G+
Sbjct: 661  PEIFGNNCPQLHGQFTWHDVISSVKLLHEISKTLKEKRFRDGALRLENSKLIYLYDEYGI 720

Query: 721  PYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFELFCS 780
            PYDS FYE KDSNFLVEEFMLLAN TVAEVISRTFPDSALLRRHPEP++RKLREFE FCS
Sbjct: 721  PYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFESFCS 780

Query: 781  RHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKDGENG 840
            +HGF LDTSSSV FQ+SLEQIR KLHDDPLLFDILISYATRPMQLATYFCSGELKDGE  
Sbjct: 781  KHGFELDTSSSVHFQQSLEQIRTKLHDDPLLFDILISYATRPMQLATYFCSGELKDGEKR 840

Query: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQMRCFTG 900
            +HYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAE++YLKHQGIIQKVN D++MRCFTG
Sbjct: 841  NHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKVYLKHQGIIQKVNSDKEMRCFTG 900

Query: 901  IHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACDKLYMWA 960
            I+FDKDAADSLEGREALS AAL+HGVPC+KLLSDVALHCN+RKLAS+H+AD C+KLYMWA
Sbjct: 901  IYFDKDAADSLEGREALSFAALKHGVPCSKLLSDVALHCNDRKLASKHIADGCEKLYMWA 960

Query: 961  LLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTLVLSFFG 1020
            LLKKK ILFSDARVLGLGPRFMS+YIQKLAIERRIYYDEVEGLAVEWLD TSTLVLSFF 
Sbjct: 961  LLKKKRILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLDTTSTLVLSFFC 1020

Query: 1021 TRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGASTGG-AAVEQESNLK 1080
            +RRS+R RGS KWKALEDVALVISPCD NV +RTLG  P+  GGAS GG AAVEQ+SNLK
Sbjct: 1021 SRRSHRSRGSVKWKALEDVALVISPCDQNVNKRTLGVCPN--GGASKGGSAAVEQDSNLK 1080

Query: 1081 SHISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIG 1120
            SH+SD G+DPAVFPLTVRLLS+IPVALHAVGGDDGPIDIG
Sbjct: 1081 SHVSDIGVDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIG 1115

BLAST of Sgr011635 vs. TAIR 10
Match: AT1G77680.1 (Ribonuclease II/R family protein )

HSP 1 Score: 988.0 bits (2553), Expect = 8.0e-288
Identity = 564/1127 (50.04%), Postives = 737/1127 (65.39%), Query Frame = 0

Query: 1    MRGAVEQFTPERNDDGEKEKKKK-RRSNRRSKQNASITTSVSCSSVNAIPGEASECMENG 60
            M+ A  + + ER ++G K+K+ + ++ NRRSKQ          SSV        E ++ G
Sbjct: 1    MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQ----------SSVPIEDAHVEESLD-G 60

Query: 61   RIDTNLTALSNYSSSMQQEYGSNHPNEHGLTRTNKIASSSLPPLHISEQGELLESQSFIN 120
            R  +   A  + SSS QQ     + +E    R + +A +S+PP+  +E G    S S + 
Sbjct: 61   RDSSRSKAKDSTSSSKQQR---PNTDELEAMRASNVAFNSMPPMR-AESGYPRRSASPL- 120

Query: 121  QHLHSSDAGGKFI-KSCPQQIACGRMPGISMNQHSPPAHETENNPQRKYFTSYWFMDDVN 180
              L S +   + + KSCP   AC + PG+    +     + E + QRK F+S+W +D V 
Sbjct: 121  --LSSPEVSKQLLSKSCPDPRACEQSPGM----NGELFQQIEGSSQRKIFSSHWSLDAVT 180

Query: 181  EGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTS 240
            E L+KG+ FKA FRVNAHNR EAYCKIDG+P D+LING   Q+RAVEGD V IK+DP + 
Sbjct: 181  EALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKLDPLSL 240

Query: 241  WTRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGKNKVD-VDVKSDSFRSSSLPDKRC 300
            W +MKG      +    E  N      EKD    + KN +D V+   D F          
Sbjct: 241  WPKMKGFVT--ESAAKPEGTN---SPPEKDDKKARQKNGIDVVEGFEDGF---------- 300

Query: 301  CSDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAHYSSNQDDVSKAIGRICAVINLY 360
             S +   + G    + + P+           LDS    +   + + S A+ ++C +++ +
Sbjct: 301  -SKNKSSVIGKGAKNGVTPS-------SPPSLDSCLGSFCEQKGNCS-AVDKLCGILSSF 360

Query: 361  PSKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIYIKEMNKKSCLS--SSSNQGYVQ 420
            P KRP G+VVA++EKS  R++IVG L+VK +     I+ KE + K C S  S S+  YVQ
Sbjct: 361  PHKRPTGQVVAVVEKSLVRDSIVGLLDVKGW-----IHYKESDPKRCKSPLSLSDDEYVQ 420

Query: 421  LMPNDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAARIHEWVEESSAPQAHVLHVL 480
            LMP D RFP ++V    LP  I+ RL+N D  +E+ELVAA+I +W E S  P A + H+ 
Sbjct: 421  LMPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLF 480

Query: 481  GRGSGVESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIPQEELQYRRDLRNLCIFTIDP 540
            GRGS +E  I+AIL++N++   +FS  SL+ LP  PW++P+EE+Q R+DLR+LC+ TIDP
Sbjct: 481  GRGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDP 540

Query: 541  SSASDLDDALSVEKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPML 600
            S+A+DLDDALSV+ L  G FRVG+HIADVS+FVLP+TALD EA+ RSTSVYL+QRKI ML
Sbjct: 541  STATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISML 600

Query: 601  PPLLSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDGL 660
            PPLLSEN+GSL+PG DRLAFS+  D+N  GDV + WIGRT+I SCCKLSY+HAQDIIDG 
Sbjct: 601  PPLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDGK 660

Query: 661  IDSASSKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLY 720
             D A      N  P LHG F W  V  SVK L EIS TL++KRFR+GAL+LENSK V+L+
Sbjct: 661  SDVAE-----NGWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLF 720

Query: 721  DEFGMPYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREF 780
            DE G+PYD      K SNFLVEEFMLLAN T AEVIS+ +  S+LLRRHPEP  RKL+EF
Sbjct: 721  DEHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYRASSLLRRHPEPNTRKLKEF 780

Query: 781  ELFCSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELK 840
            E FCS+HG  LD SSS Q Q SLE+I   L DD +  DIL +YA +PMQLA+YFC+G LK
Sbjct: 781  EGFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLK 840

Query: 841  DG-ENGSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELYLKHQGIIQKVNGDEQ 900
            D      HYALAVPLYTHFTSPLRRYPDIVVHR LAAA+EAEELY K     ++   DE 
Sbjct: 841  DSVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQ----KQTAIDEG 900

Query: 901  MRCFTGIHFDKDAADSLEGREALSSAALRHGVPCTKLLSDVALHCNNRKLASRHVADACD 960
              CFTGIHF+KDAA+S+EG+EALS AAL+HGVP T++LSDVA +CN RKLA+R V DACD
Sbjct: 901  RSCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACD 960

Query: 961  KLYMWALLKKKEILFSDARVLGLGPRFMSLYIQKLAIERRIYYDEVEGLAVEWLDATSTL 1020
            KLY W +LK+KEI   +ARV+ LG RFM++YI KL IERRIYYD++EGL  +WL+ATSTL
Sbjct: 961  KLYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTL 1020

Query: 1021 VLSFFGTRRSYRGRGSSKWKALEDVALVISPCDLNVQQRTLGGSPSELGGASTGGAAVEQ 1080
            ++    ++R  RG     +K +++   ++SPC++ V + +               A    
Sbjct: 1021 IVDKLYSKRGGRG----FFKPMKEAVYLVSPCEVCVAKCS---------------ALSVH 1048

Query: 1081 ESNLKSHISDTGIDPAVFPLTVRLLSSIPVALHAVGGDDGPIDIGVR 1122
            ++     +S   + PAVFPLT++L S+IPV LHAVGGDDGP+DIG R
Sbjct: 1081 DTESPEAVSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGAR 1048

BLAST of Sgr011635 vs. TAIR 10
Match: AT2G17510.1 (ribonuclease II family protein )

HSP 1 Score: 296.6 bits (758), Expect = 1.1e-79
Identity = 211/723 (29.18%), Postives = 333/723 (46.06%), Query Frame = 0

Query: 156 AHETENNPQRKYFTSYWFMDDVNEGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLIN 215
           A ++  + ++  +  +  M ++  GL +G   +   RVN  N  EAY   + +  +++I 
Sbjct: 204 ADDSRPSKRKLIYQEHKPMSEITAGLHRGIYHQGKLRVNRFNPYEAYVGSESIGEEIIIY 263

Query: 216 GIASQNRAVEGDIVAIKVDPFTSWTRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGK 275
           G ++ NRA +GDIVA+++ P   W   K  + A              E++E+D       
Sbjct: 264 GRSNMNRAFDGDIVAVELLPRDQWQDEKALSIAE-------------EDDEEDDTVHLAP 323

Query: 276 NKVDVDVKSDSFRSSSLPDKRCCSDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAH 335
           + VD     D+ R+S+L                                         +H
Sbjct: 324 DNVD-----DAPRTSNL-----------------------------------------SH 383

Query: 336 YSSNQDDVSKAIGRICAVINLYPSKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIY 395
            +S   + +                RP+GRVV ++ ++                     Y
Sbjct: 384 ETSGDKNAAPV--------------RPSGRVVGVIRRN------------------WHSY 443

Query: 396 IKEMNKKSCLSSSSNQGYVQLMPNDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAA 455
              +   S  + S    +   +  D R P + +    L N +  R            +  
Sbjct: 444 CGSLEPMSLPAGSGGTAHALFVSKDRRIPKIRINTRQLQNLLDMR------------IVV 503

Query: 456 RIHEWVEESSAPQAHVLHVLGRGSGVESHIDAILFENAIRTCEFSHDSLSCLPHTPWKIP 515
            +  W  +S  P  H +  +G+    E+  + +L EN +    FS   L+CLP  PW + 
Sbjct: 504 AVDSWDRQSRYPSGHYVRPIGKIGDKETETEVVLIENDVDYSPFSSQVLACLPPLPWSVS 563

Query: 516 QEELQ--YRRDLRNLCIFTIDPSSASDLDDALSVEKLANGIFRVGIHIADVSHFVLPDTA 575
            E++    R+DLR+L +F++DP    D+DDAL    L NG F +G+HIADV++FV P T 
Sbjct: 564 SEDVSNPVRQDLRHLLVFSVDPPGCKDIDDALHCTSLPNGNFELGVHIADVTNFVHPGTP 623

Query: 576 LDKEAQIRSTSVYLLQRKIPMLPPLLSENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIG 635
           LD EA  R TSVYL++R+I MLP  L+E+I SL   V+RLAFS+  +++   ++ +    
Sbjct: 624 LDDEASKRGTSVYLVERRIDMLPKPLTEDICSLRADVERLAFSVIWEMSPDAEIISTRFT 683

Query: 636 RTVICSCCKLSYEHAQDIIDG--LIDSASSKILGNHCPQLHGQFAWPGVISSVKILYEIS 695
           +++I S   LSY  AQ  +D   L DS                     + + ++ +  ++
Sbjct: 684 KSIIKSSAALSYIEAQARMDDSRLTDS---------------------LTTDLRNMNTLA 743

Query: 696 KTLKEKRFRDGALRLENSKRVYLYD-EFGMPYDSTFYEHKDSNFLVEEFMLLANTTVAEV 755
           K ++++R   GAL L +++  +  D E   P +   Y+  ++N +VEEFML AN +VA  
Sbjct: 744 KIMRQRRIDRGALTLASAEVKFDIDPENHDPLNIGMYQILEANQMVEEFMLAANVSVAGQ 798

Query: 756 ISRTFPDSALLRRHPEPIMRKLREFELFCSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPL 815
           I + FP  +LLRRHP P    L       +  G  LD SSS     SL++    + +DP 
Sbjct: 804 ILKLFPSCSLLRRHPTPTREMLEPLLRTAAAIGLTLDVSSSKALADSLDR---AVGEDPY 798

Query: 816 LFDILISYATRPMQLATYFCSGELKDGENGSHYALAVPLYTHFTSPLRRYPDIVVHRTLA 874
              ++   ATR M  A YFCSG+L   E   HY LA PLYTHFTSP+RRY D+ VHR LA
Sbjct: 864 FNKLIRILATRCMTQAVYFCSGDLSPPEY-HHYGLAAPLYTHFTSPIRRYADVFVHRLLA 798

BLAST of Sgr011635 vs. TAIR 10
Match: AT2G17510.2 (ribonuclease II family protein )

HSP 1 Score: 273.9 bits (699), Expect = 7.7e-73
Identity = 212/757 (28.01%), Postives = 335/757 (44.25%), Query Frame = 0

Query: 156 AHETENNPQRKYFTSYWFMDDVNEGLQKGDIFKAFFRVNAHNRLEAYCKIDGLPVDVLIN 215
           A ++  + ++  +  +  M ++  GL +G   +   RVN  N  EAY   + +  +++I 
Sbjct: 249 ADDSRPSKRKLIYQEHKPMSEITAGLHRGIYHQGKLRVNRFNPYEAYVGSESIGEEIIIY 308

Query: 216 GIASQNRAVEGDIVAIKVDPFTSWTRMKGTTEAHNNMHPMEDANLHAEENEKDGHSCKGK 275
           G ++ NRA +GDIVA+++ P   W   K  + A              E++E+D       
Sbjct: 309 GRSNMNRAFDGDIVAVELLPRDQWQDEKALSIAE-------------EDDEEDDTVHLAP 368

Query: 276 NKVDVDVKSDSFRSSSLPDKRCCSDDNIVLDGTACDDDLLPNYEQCDVYQSSVLDSSQAH 335
           + VD     D+ R+S+L                                         +H
Sbjct: 369 DNVD-----DAPRTSNL-----------------------------------------SH 428

Query: 336 YSSNQDDVSKAIGRICAVINLYPSKRPAGRVVAILEKSRQREAIVGHLNVKKFLSFQEIY 395
            +S   + +                RP+GRVV ++ ++                     Y
Sbjct: 429 ETSGDKNAAPV--------------RPSGRVVGVIRRN------------------WHSY 488

Query: 396 IKEMNKKSCLSSSSNQGYVQLMPNDARFPLMMVLAGDLPNCIKKRLDNGDVTVESELVAA 455
              +   S  + S    +   +  D R P + +    L N +  R            +  
Sbjct: 489 CGSLEPMSLPAGSGGTAHALFVSKDRRIPKIRINTRQLQNLLDMR------------IVV 548

Query: 456 RIHEWVEESSAPQAHVLHVLGR------GSGVESHID----------------AILFENA 515
            +  W  +S  P  H +  +G+       + V  HI+                 +L EN 
Sbjct: 549 AVDSWDRQSRYPSGHYVRPIGKIGDKETETEVRDHINLFDSILVGVRWARVGKVVLIEND 608

Query: 516 IRTCEFSHDSLSCLPHTPWKIPQEELQ--YRRDLRNLCIFTIDPSSASDLDDALSVEKLA 575
           +    FS   L+CLP  PW +  E++    R+DLR+L +F++DP    D+DDAL    L 
Sbjct: 609 VDYSPFSSQVLACLPPLPWSVSSEDVSNPVRQDLRHLLVFSVDPPGCKDIDDALHCTSLP 668

Query: 576 NGIFRVGI------------HIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLL 635
           NG F +G+            +IADV++FV P T LD EA  R TSVYL++R+I MLP  L
Sbjct: 669 NGNFELGVRILESSDSHKYDYIADVTNFVHPGTPLDDEASKRGTSVYLVERRIDMLPKPL 728

Query: 636 SENIGSLNPGVDRLAFSLFLDINHCGDVKNCWIGRTVICSCCKLSYEHAQDIIDG--LID 695
           +E+I SL   V+RLAFS+  +++   ++ +    +++I S   LSY  AQ  +D   L D
Sbjct: 729 TEDICSLRADVERLAFSVIWEMSPDAEIISTRFTKSIIKSSAALSYIEAQARMDDSRLTD 788

Query: 696 SASSKILGNHCPQLHGQFAWPGVISSVKILYEISKTLKEKRFRDGALRLENSKRVYLYD- 755
           S                     + + ++ +  ++K ++++R   GAL L +++  +  D 
Sbjct: 789 S---------------------LTTDLRNMNTLAKIMRQRRIDRGALTLASAEVKFDIDP 848

Query: 756 EFGMPYDSTFYEHKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPIMRKLREFE 815
           E   P +   Y+  ++N +VEEFML AN +VA  I + FP  +LLRRHP P    L    
Sbjct: 849 ENHDPLNIGMYQILEANQMVEEFMLAANVSVAGQILKLFPSCSLLRRHPTPTREMLEPLL 877

Query: 816 LFCSRHGFVLDTSSSVQFQRSLEQIRLKLHDDPLLFDILISYATRPMQLATYFCSGELKD 874
              +  G  LD SSS     SL++    + +DP    ++   ATR M  A YFCSG+L  
Sbjct: 909 RTAAAIGLTLDVSSSKALADSLDR---AVGEDPYFNKLIRILATRCMTQAVYFCSGDLSP 877

BLAST of Sgr011635 vs. TAIR 10
Match: AT4G08240.2 (unknown protein; Has 33 Blast hits to 33 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 33; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 124.0 bits (310), Expect = 9.9e-28
Identity = 68/133 (51.13%), Postives = 90/133 (67.67%), Query Frame = 0

Query: 1152 MRGTGGPLLCIGDLLCDVGEEDGGEGKGSHETPKSLPSSSSSSTASISSLPDVSEPPDLA 1211
            MRG GGPLL IGDLL D+G+E  G    +H  P+    S S  T        +S P DL 
Sbjct: 1    MRGVGGPLLSIGDLLADLGDET-GHSPQNHPNPEVSSKSYSDDT--------ISGPLDLT 60

Query: 1212 RLFQENYDQLNKSFDDNDHSWTALTLKMCSALETANKLVESTNSNSRFLLEKIVELEQNL 1271
            RLFQENYD+LN +F  +DHSWT+LTL++C++LETANKLV +T +N+R L EK+ ELE+ +
Sbjct: 61   RLFQENYDKLNDAFAGSDHSWTSLTLELCTSLETANKLVHATTTNARLLSEKVEELEKIV 120

Query: 1272 EKGDSTREAAMAI 1285
            ++GDS   AA  +
Sbjct: 121  KRGDSAVAAARTV 124

BLAST of Sgr011635 vs. TAIR 10
Match: AT4G08240.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 124.0 bits (310), Expect = 9.9e-28
Identity = 68/133 (51.13%), Postives = 90/133 (67.67%), Query Frame = 0

Query: 1152 MRGTGGPLLCIGDLLCDVGEEDGGEGKGSHETPKSLPSSSSSSTASISSLPDVSEPPDLA 1211
            MRG GGPLL IGDLL D+G+E  G    +H  P+    S S  T        +S P DL 
Sbjct: 1    MRGVGGPLLSIGDLLADLGDET-GHSPQNHPNPEVSSKSYSDDT--------ISGPLDLT 60

Query: 1212 RLFQENYDQLNKSFDDNDHSWTALTLKMCSALETANKLVESTNSNSRFLLEKIVELEQNL 1271
            RLFQENYD+LN +F  +DHSWT+LTL++C++LETANKLV +T +N+R L EK+ ELE+ +
Sbjct: 61   RLFQENYDKLNDAFAGSDHSWTSLTLELCTSLETANKLVHATTTNARLLSEKVEELEKIV 120

Query: 1272 EKGDSTREAAMAI 1285
            ++GDS   AA  +
Sbjct: 121  KRGDSAVAAARTV 124