Sgr004546 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr004546
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	alpha-glucosidase 2
Location	tig00003038: 50753 .. 52811 (+)
RNA-Seq Expression	Sgr004546
Synteny	Sgr004546

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGAAGAAGCATCAGTGTTGAGTGGGCTAGTGGGTGGAGGTGGAGCATGGGGATATATTCCTCGGCTTAATCGGACTCCAAATTTTAAATACGACATTTGTAGAAGAAGAATTTCGGGTGCTCCTGCTGGGGATTCGAAGAAGCTTGATCTTGTGGCTTCTCTCTTTTGCTTTTCCAGGTTTTTGCTAAATATTTTTACCTTATCTCTGGCCCCGAAGATGGAGACTAGTTCTCTTTAGTTTGGATTTCGATAGCTTTCATTTTCAATTCTGAAGATTAAAGGCTGTCTTGTTGATTGGAGTCATTAAACAAAGGGAATTTGTTCTCCGATGATATCTAAATTCGTTGATTGAACAACTAACTCAATGTTTCGCAATTTTAGAGCATAGAGGAAGTGTTTTTTCTTCCCAAGAAAAGTTTTATCCCAATGTAGTCGATAAGTCACTGATAATGGAAAATCCTCGTCCCCCTCTGAGGAAGGAGAACTTCTAATAATCTGTCAGATTTCAAAGTTCAGCTCGTAGAGTTTAATGACCTTGAAAATCAGTCTCTCGAAGACCAGAACGATTTGTTTGCAAAAATTTAAGGAATGGAAGGACCTCAATGTGAGAAAGTCCCCATCTATCCCTGATTAATTTTCTTGTTGAAGTCCCCATCTATCCCTGATTAATTTTCTTGTTGTATGATGTGCCAAATCTTGGTCTGCAGAATTTTAGCTGTTTCTTTCGATTTACCTGCCTATAAGTTTTGTTTTCTTTATATCTGTACTACCCAAAGAAAGCTTCTGAAAACTGTGCACCGAATCATGAGCAATGAAGGCATTAATATAACCGAAGAAGTCCGAGACACTATATACTCAACATGAATTGCCATGGTGGTATACTGATTCCAAATTGTGGCTATTTGATCAAGGGGGCTTAACTCTCTGAATGGTTTTTTTCTTTTTTTTTTCCCTCTTGATTTTTTGGGGTACAGGAGAAAGAGGATCAGCAAGAAGTTGATTTCTGAAAGGTTTATATGTAAAATGGTGGATACCAAAGAGAAAGGAGCCACAACAGATGCTATCTCAGGGAATATGATTTTTGAGCTTATACTGGAGGATGGGGTTTTTCGATTTGATTGTTCTTCACTTGATAGAGCTGCAGCTCATCCAAGTTTTTCTTTCTTAAAATCCAAGGACAGAGACACACCAATTTCTAGCCAGAAGCTTCCTACATATATTCCTGTGTTTGAGTGTCGTCTTGGCCAGCAGATTGTTAAACTGGAGGTCCGTTTTTGTATAAATATTATCGTTAGTTTTTGGGCATCTGGCGGTTATGATTTCTGGTTGTGAAAGGTTCCTGCATCATTTTTATAGTTGTTATGTTTATATCTTCTTCTAGTATACTTGACAAGTTTTGAAATCCATTTCAAGTAATTTGACAAACTTGACTTCAACAGCTTCCTGCTGGTACCTCCTTTTATGGAACTGGGGAAGTTAGTGGACAGCTTGAGCGAACCGGGAAAAGAGTAAGTTAATAATTTGGTATACTTGATATTAGTTGGTTAATGCTTCTTGATTTTAGAAGTTCCCTCCCTTGTAGATTTTCACTTGGAATACAGATGCATATGGATATGGTTCCGGAACTACATCCTTGTACCAAACACATCCATGGGTGTTAGCCATTCTTCCAAATGGGGAGGCACTAGGTGTTCTTGCTGACACAACCCTGCGTTGTGAGGTGCTTCAACAATCTTGTTGTATATTCTTTTTCGAGAATATTACATTACAAATATGGAATGGGAATTTGAACTTATAACTTTTTAGAGGAAGTAGAGATGCCTTAACCACTGAGCTATGCTTTGGTTGGCATTGGTCTTGTTGTATATGGAAGTTACAATTTTTGGATATCTCACTTGTTGCTATTATAACAGTTAAAAATTCTGTATTCTTGCAGATTGATCTGAGAAAAGATTCAATAATACAGTTTGTTGCTCCTTCCTCATATCCTGTCATTACGTTCGGTCCATTTTCCTCACCAACTGCAGTTTTAAAGTCCTTCTGTAGTGCAGTTG

mRNA sequence

ATGATGGAAGAAGCATCAGTGTTGAGTGGGCTAGTGGGTGGAGGTGGAGCATGGGGATATATTCCTCGGCTTAATCGGACTCCAAATTTTAAATACGACATTTGTAGAAGAAGAATTTCGGGTGCTCCTGCTGGGGATTCGAAGAAGCTTGATCTTGTGGCTTCTCTCTTTTGCTTTTCCAGGAGAAAGAGGATCAGCAAGAAGTTGATTTCTGAAAGGTTTATATGTAAAATGGTGGATACCAAAGAGAAAGGAGCCACAACAGATGCTATCTCAGGGAATATGATTTTTGAGCTTATACTGGAGGATGGGGTTTTTCGATTTGATTGTTCTTCACTTGATAGAGCTGCAGCTCATCCAAGTTTTTCTTTCTTAAAATCCAAGGACAGAGACACACCAATTTCTAGCCAGAAGCTTCCTACATATATTCCTGTGTTTGAGTGTCGTCTTGGCCAGCAGATTGTTAAACTGGAGCTTCCTGCTGGTACCTCCTTTTATGGAACTGGGGAAGTTAGTGGACAGCTTGAGCGAACCGGGAAAAGAATTTTCACTTGGAATACAGATGCATATGGATATGGTTCCGGAACTACATCCTTGTACCAAACACATCCATGGGTGTTAGCCATTCTTCCAAATGGGGAGGCACTAGGTGTTCTTGCTGACACAACCCTGCGTTGTGAGATTGATCTGAGAAAAGATTCAATAATACAGTTTGTTGCTCCTTCCTCATATCCTGTCATTACGTTCGGTCCATTTTCCTCACCAACTGCAGTTTTAAAGTCCTTCTGTAGTGCAGTTG

Coding sequence (CDS)

ATGATGGAAGAAGCATCAGTGTTGAGTGGGCTAGTGGGTGGAGGTGGAGCATGGGGATATATTCCTCGGCTTAATCGGACTCCAAATTTTAAATACGACATTTGTAGAAGAAGAATTTCGGGTGCTCCTGCTGGGGATTCGAAGAAGCTTGATCTTGTGGCTTCTCTCTTTTGCTTTTCCAGGAGAAAGAGGATCAGCAAGAAGTTGATTTCTGAAAGGTTTATATGTAAAATGGTGGATACCAAAGAGAAAGGAGCCACAACAGATGCTATCTCAGGGAATATGATTTTTGAGCTTATACTGGAGGATGGGGTTTTTCGATTTGATTGTTCTTCACTTGATAGAGCTGCAGCTCATCCAAGTTTTTCTTTCTTAAAATCCAAGGACAGAGACACACCAATTTCTAGCCAGAAGCTTCCTACATATATTCCTGTGTTTGAGTGTCGTCTTGGCCAGCAGATTGTTAAACTGGAGCTTCCTGCTGGTACCTCCTTTTATGGAACTGGGGAAGTTAGTGGACAGCTTGAGCGAACCGGGAAAAGAATTTTCACTTGGAATACAGATGCATATGGATATGGTTCCGGAACTACATCCTTGTACCAAACACATCCATGGGTGTTAGCCATTCTTCCAAATGGGGAGGCACTAGGTGTTCTTGCTGACACAACCCTGCGTTGTGAGATTGATCTGAGAAAAGATTCAATAATACAGTTTGTTGCTCCTTCCTCATATCCTGTCATTACGTTCGGTCCATTTTCCTCACCAACTGCAGTTTTAAAGTCCTTCTGTAGTGCAGTTG

Protein sequence

MMEEASVLSGLVGGGGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCFSRRKRISKKLISERFICKMVDTKEKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHPSFSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGKRIFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVAPSSYPVITFGPFSSPTAVLKSFCSAVX

Homology

[Close]

BLAST of Sgr004546 vs. NCBI nr
Match: XP_022145307.1 (uncharacterized protein LOC111014792 [Momordica charantia])

HSP 1 Score: 427.9 bits (1099), Expect = 6.2e-116
Identity = 216/265 (81.51%), Postives = 230/265 (86.79%), Query Frame = 0


Query: 2   MEEASVLSGLVGGGGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCFSR 61
           MEEASV SGLV  GGAWGYIPRLNRTPNF  +     +SG P  D KKL L        R
Sbjct: 1   MEEASVSSGLV--GGAWGYIPRLNRTPNFLSNFSATTVSGVPPRDLKKLHLP-----LPR 60

Query: 62  RKRISKKLISERFICKMVDTKEKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHPS 121
           RK+I++KLIS  F CKM D KE+G TTD ISGNMIFE ILEDGVFRFDCSS DRAAA+PS
Sbjct: 61  RKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYPS 120

Query: 122 FSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGKR 181
           FSFL+S+DRDTPISSQKLPTY+PVFEC LGQQIVKLELPAGTSFYGTGEVSGQLERTGKR
Sbjct: 121 FSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGKR 180

Query: 182 IFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVAP 241
           IFTWNTDAYGYG+GTTSLYQ HPWVLAILPNG+ALGVLADT+LRCEIDLRKDSIIQFVAP
Sbjct: 181 IFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVAP 240

Query: 242 SSYPVITFGPFSSPTAVLKSFCSAV 267
           SSYPVITFGPFSSPTAVLK+F SAV
Sbjct: 241 SSYPVITFGPFSSPTAVLKAFSSAV 258

[Close]

BLAST of Sgr004546 vs. NCBI nr
Match: XP_038876569.1 (alpha-glucosidase 2 [Benincasa hispida])

HSP 1 Score: 414.1 bits (1063), Expect = 9.3e-112
Identity = 215/268 (80.22%), Postives = 229/268 (85.45%), Query Frame = 0


Query: 1   MMEEASVLSGL-VGGGGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCF 60
           M+EEASVLSGL V  GG+ GYIP L+RTPN  + I    ISGA + DSKKLD       F
Sbjct: 1   MIEEASVLSGLVVPSGGSSGYIPWLSRTPNLIFRISSTTISGACSWDSKKLD-------F 60

Query: 61  SRRKRISKKLISERFICKMVDTKEKGATTD-AISGNMIFELILEDGVFRFDCSSLDRAAA 120
            RRKR +KKLISE F CKM + KEKG T D  ISGNMIFE ILEDGVFRFDCS+ DRAAA
Sbjct: 61  PRRKRTNKKLISENFTCKMGNVKEKGTTKDTTISGNMIFEPILEDGVFRFDCSANDRAAA 120

Query: 121 HPSFSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERT 180
           +PSFSFLKSKDRDTPISSQKLPTYIPVFEC LGQQIVKLELPAGTS YGTGEVSGQLERT
Sbjct: 121 YPSFSFLKSKDRDTPISSQKLPTYIPVFECLLGQQIVKLELPAGTSLYGTGEVSGQLERT 180

Query: 181 GKRIFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQF 240
           GKRIFTWNTDAYGYGSGTTSLYQ+HPWVLAILPNGEALGVLADT+LRCEIDLR+DS+IQF
Sbjct: 181 GKRIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGVLADTSLRCEIDLREDSVIQF 240

Query: 241 VAPSSYPVITFGPFSSPTAVLKSFCSAV 267
           +APSSYPVITFGPFSSP A LKSF  AV
Sbjct: 241 IAPSSYPVITFGPFSSPAAALKSFSRAV 261

[Close]

BLAST of Sgr004546 vs. NCBI nr
Match: XP_022923248.1 (uncharacterized protein LOC111430993 isoform X2 [Cucurbita moschata])

HSP 1 Score: 413.7 bits (1062), Expect = 1.2e-111
Identity = 211/266 (79.32%), Postives = 230/266 (86.47%), Query Frame = 0


Query: 2   MEEASVLSGLVGG-GGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCFS 61
           MEE+SVLSGLV   G + GYIP LNRTPN  + IC  RISG+ + DS KL        FS
Sbjct: 1   MEESSVLSGLVARCGRSSGYIPWLNRTPNSIHRICTTRISGSRSCDSNKLH-------FS 60

Query: 62  RRKRISKKLISERFICKMVDTKEKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHP 121
           RRK  +KKLIS +F CKM +TKEKG T D +SGNMIFE ILEDGVFRFDCS+ DRAAA+P
Sbjct: 61  RRKWTNKKLISGKFACKMGNTKEKGITIDTVSGNMIFEPILEDGVFRFDCSANDRAAAYP 120

Query: 122 SFSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 181
           SFSF+KSKDRDTPISSQKL TY+PVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 121 SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 180

Query: 182 RIFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVA 241
           RIFTWNTDAYGYGSGTTSLYQ+HPWVLAILPNGEALGVLADT+LRCEIDLR+DSIIQF+A
Sbjct: 181 RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGVLADTSLRCEIDLREDSIIQFIA 240

Query: 242 PSSYPVITFGPFSSPTAVLKSFCSAV 267
           PSSYPVITFGPFSSP+AVLKSF  AV
Sbjct: 241 PSSYPVITFGPFSSPSAVLKSFSKAV 259

[Close]

BLAST of Sgr004546 vs. NCBI nr
Match: KAG6577761.1 (hypothetical protein SDJN03_25335, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 413.7 bits (1062), Expect = 1.2e-111
Identity = 211/266 (79.32%), Postives = 230/266 (86.47%), Query Frame = 0


Query: 2   MEEASVLSGLVGG-GGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCFS 61
           MEE+SVLSGLV   G + GYIP LNRTPN  + IC  RISG+ + DS KL        FS
Sbjct: 1   MEESSVLSGLVARCGRSSGYIPWLNRTPNSIHRICTTRISGSRSCDSNKLH-------FS 60

Query: 62  RRKRISKKLISERFICKMVDTKEKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHP 121
           RRK  +KKLIS +F CKM +TKEKG T D +SGNMIFE ILEDGVFRFDCS+ DRAAA+P
Sbjct: 61  RRKWTNKKLISGKFACKMGNTKEKGITIDTVSGNMIFEPILEDGVFRFDCSANDRAAAYP 120

Query: 122 SFSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 181
           SFSF+KSKDRDTPISSQKL TY+PVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 121 SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 180

Query: 182 RIFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVA 241
           RIFTWNTDAYGYGSGTTSLYQ+HPWVLAILPNGEALGVLADT+LRCEIDLR+DSIIQF+A
Sbjct: 181 RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGVLADTSLRCEIDLREDSIIQFIA 240

Query: 242 PSSYPVITFGPFSSPTAVLKSFCSAV 267
           PSSYPVITFGPFSSP+AVLKSF  AV
Sbjct: 241 PSSYPVITFGPFSSPSAVLKSFSKAV 259

[Close]

BLAST of Sgr004546 vs. NCBI nr
Match: XP_022923247.1 (uncharacterized protein LOC111430993 isoform X1 [Cucurbita moschata])

HSP 1 Score: 413.7 bits (1062), Expect = 1.2e-111
Identity = 211/266 (79.32%), Postives = 230/266 (86.47%), Query Frame = 0


Query: 2   MEEASVLSGLVGG-GGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCFS 61
           MEE+SVLSGLV   G + GYIP LNRTPN  + IC  RISG+ + DS KL        FS
Sbjct: 1   MEESSVLSGLVARCGRSSGYIPWLNRTPNSIHRICTTRISGSRSCDSNKLH-------FS 60

Query: 62  RRKRISKKLISERFICKMVDTKEKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHP 121
           RRK  +KKLIS +F CKM +TKEKG T D +SGNMIFE ILEDGVFRFDCS+ DRAAA+P
Sbjct: 61  RRKWTNKKLISGKFACKMGNTKEKGITIDTVSGNMIFEPILEDGVFRFDCSANDRAAAYP 120

Query: 122 SFSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 181
           SFSF+KSKDRDTPISSQKL TY+PVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 121 SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 180

Query: 182 RIFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVA 241
           RIFTWNTDAYGYGSGTTSLYQ+HPWVLAILPNGEALGVLADT+LRCEIDLR+DSIIQF+A
Sbjct: 181 RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGVLADTSLRCEIDLREDSIIQFIA 240

Query: 242 PSSYPVITFGPFSSPTAVLKSFCSAV 267
           PSSYPVITFGPFSSP+AVLKSF  AV
Sbjct: 241 PSSYPVITFGPFSSPSAVLKSFSKAV 259

[Close]

BLAST of Sgr004546 vs. ExPASy Swiss-Prot
Match: Q9F234 (Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.2e-06
Identity = 30/93 (32.26%), Postives = 49/93 (52.69%), Query Frame = 0


Query: 165 FYGTGEVSGQLERTGKRIFTWNTDAYG-YGSGTTSLYQTHPWVLAILPNGEALGVLADTT 224
           FYG GE +G L++ G+ +  WNTD Y  +   T  LYQ+HP+ + +  NG A G+  D T
Sbjct: 149 FYGFGEKTGFLDKRGETMTMWNTDVYAPHNPETDPLYQSHPYFMTV-RNGSAHGIFFDNT 208

Query: 225 LRCEIDLRKDSIIQFVAPSSYPVITFGPFSSPT 257
            +   D  + +  ++   +    I +  F+ PT
Sbjct: 209 YKTTFDF-QTATDEYCFSAEGGAIDYYVFAGPT 239

[Close]

BLAST of Sgr004546 vs. ExPASy TrEMBL
Match: A0A6J1CW78 (uncharacterized protein LOC111014792 OS=Momordica charantia OX=3673 GN=LOC111014792 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 3.0e-116
Identity = 216/265 (81.51%), Postives = 230/265 (86.79%), Query Frame = 0


Query: 2   MEEASVLSGLVGGGGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCFSR 61
           MEEASV SGLV  GGAWGYIPRLNRTPNF  +     +SG P  D KKL L        R
Sbjct: 1   MEEASVSSGLV--GGAWGYIPRLNRTPNFLSNFSATTVSGVPPRDLKKLHLP-----LPR 60

Query: 62  RKRISKKLISERFICKMVDTKEKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHPS 121
           RK+I++KLIS  F CKM D KE+G TTD ISGNMIFE ILEDGVFRFDCSS DRAAA+PS
Sbjct: 61  RKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYPS 120

Query: 122 FSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGKR 181
           FSFL+S+DRDTPISSQKLPTY+PVFEC LGQQIVKLELPAGTSFYGTGEVSGQLERTGKR
Sbjct: 121 FSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGKR 180

Query: 182 IFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVAP 241
           IFTWNTDAYGYG+GTTSLYQ HPWVLAILPNG+ALGVLADT+LRCEIDLRKDSIIQFVAP
Sbjct: 181 IFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVAP 240

Query: 242 SSYPVITFGPFSSPTAVLKSFCSAV 267
           SSYPVITFGPFSSPTAVLK+F SAV
Sbjct: 241 SSYPVITFGPFSSPTAVLKAFSSAV 258

[Close]

BLAST of Sgr004546 vs. ExPASy TrEMBL
Match: A0A6J1E5M6 (uncharacterized protein LOC111430993 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430993 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 5.9e-112
Identity = 211/266 (79.32%), Postives = 230/266 (86.47%), Query Frame = 0


Query: 2   MEEASVLSGLVGG-GGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCFS 61
           MEE+SVLSGLV   G + GYIP LNRTPN  + IC  RISG+ + DS KL        FS
Sbjct: 1   MEESSVLSGLVARCGRSSGYIPWLNRTPNSIHRICTTRISGSRSCDSNKLH-------FS 60

Query: 62  RRKRISKKLISERFICKMVDTKEKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHP 121
           RRK  +KKLIS +F CKM +TKEKG T D +SGNMIFE ILEDGVFRFDCS+ DRAAA+P
Sbjct: 61  RRKWTNKKLISGKFACKMGNTKEKGITIDTVSGNMIFEPILEDGVFRFDCSANDRAAAYP 120

Query: 122 SFSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 181
           SFSF+KSKDRDTPISSQKL TY+PVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 121 SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 180

Query: 182 RIFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVA 241
           RIFTWNTDAYGYGSGTTSLYQ+HPWVLAILPNGEALGVLADT+LRCEIDLR+DSIIQF+A
Sbjct: 181 RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGVLADTSLRCEIDLREDSIIQFIA 240

Query: 242 PSSYPVITFGPFSSPTAVLKSFCSAV 267
           PSSYPVITFGPFSSP+AVLKSF  AV
Sbjct: 241 PSSYPVITFGPFSSPSAVLKSFSKAV 259

[Close]

BLAST of Sgr004546 vs. ExPASy TrEMBL
Match: A0A6J1E909 (uncharacterized protein LOC111430993 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430993 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 5.9e-112
Identity = 211/266 (79.32%), Postives = 230/266 (86.47%), Query Frame = 0


Query: 2   MEEASVLSGLVGG-GGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCFS 61
           MEE+SVLSGLV   G + GYIP LNRTPN  + IC  RISG+ + DS KL        FS
Sbjct: 1   MEESSVLSGLVARCGRSSGYIPWLNRTPNSIHRICTTRISGSRSCDSNKLH-------FS 60

Query: 62  RRKRISKKLISERFICKMVDTKEKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHP 121
           RRK  +KKLIS +F CKM +TKEKG T D +SGNMIFE ILEDGVFRFDCS+ DRAAA+P
Sbjct: 61  RRKWTNKKLISGKFACKMGNTKEKGITIDTVSGNMIFEPILEDGVFRFDCSANDRAAAYP 120

Query: 122 SFSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 181
           SFSF+KSKDRDTPISSQKL TY+PVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 121 SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 180

Query: 182 RIFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVA 241
           RIFTWNTDAYGYGSGTTSLYQ+HPWVLAILPNGEALGVLADT+LRCEIDLR+DSIIQF+A
Sbjct: 181 RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGVLADTSLRCEIDLREDSIIQFIA 240

Query: 242 PSSYPVITFGPFSSPTAVLKSFCSAV 267
           PSSYPVITFGPFSSP+AVLKSF  AV
Sbjct: 241 PSSYPVITFGPFSSPSAVLKSFSKAV 259

[Close]

BLAST of Sgr004546 vs. ExPASy TrEMBL
Match: A0A6J1HNP7 (uncharacterized protein LOC111465248 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465248 PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 7.7e-112
Identity = 212/266 (79.70%), Postives = 229/266 (86.09%), Query Frame = 0


Query: 2   MEEASVLSGLVGG-GGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCFS 61
           MEE+SVLSGLV   G + GYIP LNRTP+F   IC  R SGA + DSKKL        FS
Sbjct: 1   MEESSVLSGLVARCGRSSGYIPWLNRTPDFISRICTTRTSGARSCDSKKLH-------FS 60

Query: 62  RRKRISKKLISERFICKMVDTKEKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHP 121
           RRK  +KKLIS +F CKM +TKEKG T D ISGNMIFE ILEDGVFRFDCS+ DRAAA+P
Sbjct: 61  RRKWTNKKLISGKFTCKMGNTKEKGITIDTISGNMIFEPILEDGVFRFDCSANDRAAAYP 120

Query: 122 SFSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 181
           SFSF+KSKDRDTPISSQKL TY+PVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 121 SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 180

Query: 182 RIFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVA 241
           RIFTWNTDAYGYGSGTTSLYQ+HPWVLAILPNGEALG+LADT LRCEIDLR+DSIIQF+A
Sbjct: 181 RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGILADTFLRCEIDLREDSIIQFIA 240

Query: 242 PSSYPVITFGPFSSPTAVLKSFCSAV 267
           PSSYPVITFGPFSSP+AVLKSF  AV
Sbjct: 241 PSSYPVITFGPFSSPSAVLKSFSKAV 259

[Close]

BLAST of Sgr004546 vs. ExPASy TrEMBL
Match: A0A6J1HQT7 (uncharacterized protein LOC111465248 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465248 PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 7.7e-112
Identity = 212/266 (79.70%), Postives = 229/266 (86.09%), Query Frame = 0


Query: 2   MEEASVLSGLVGG-GGAWGYIPRLNRTPNFKYDICRRRISGAPAGDSKKLDLVASLFCFS 61
           MEE+SVLSGLV   G + GYIP LNRTP+F   IC  R SGA + DSKKL        FS
Sbjct: 1   MEESSVLSGLVARCGRSSGYIPWLNRTPDFISRICTTRTSGARSCDSKKLH-------FS 60

Query: 62  RRKRISKKLISERFICKMVDTKEKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHP 121
           RRK  +KKLIS +F CKM +TKEKG T D ISGNMIFE ILEDGVFRFDCS+ DRAAA+P
Sbjct: 61  RRKWTNKKLISGKFTCKMGNTKEKGITIDTISGNMIFEPILEDGVFRFDCSANDRAAAYP 120

Query: 122 SFSFLKSKDRDTPISSQKLPTYIPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 181
           SFSF+KSKDRDTPISSQKL TY+PVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 121 SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 180

Query: 182 RIFTWNTDAYGYGSGTTSLYQTHPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVA 241
           RIFTWNTDAYGYGSGTTSLYQ+HPWVLAILPNGEALG+LADT LRCEIDLR+DSIIQF+A
Sbjct: 181 RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGILADTFLRCEIDLREDSIIQFIA 240

Query: 242 PSSYPVITFGPFSSPTAVLKSFCSAV 267
           PSSYPVITFGPFSSP+AVLKSF  AV
Sbjct: 241 PSSYPVITFGPFSSPSAVLKSFSKAV 259

[Close]

BLAST of Sgr004546 vs. TAIR 10
Match: AT3G23640.1 (heteroglycan glucosidase 1 )

HSP 1 Score: 256.9 bits (655), Expect = 1.8e-68
Identity = 123/184 (66.85%), Postives = 142/184 (77.17%), Query Frame = 0


Query: 83  EKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHPSFSFLKSKDRDTPISSQKLPTY 142
           +   T +  S +MIFE ILE GVFRFDCS   R AA PS SF  SKDR+ PI S  +P Y
Sbjct: 6   DSSETVEMTSTDMIFEPILEHGVFRFDCSVDHRKAAFPSVSFKNSKDREVPIVSHIVPAY 65

Query: 143 IPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGKRIFTWNTDAYGYGSGTTSLYQT 202
           IP   C   QQ+V  E   GTSFYGTGEVSGQLERTGKR+FTWNTDA+GYGSGTTSLYQ+
Sbjct: 66  IPTCGCLQDQQVVTFEFSPGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGSGTTSLYQS 125

Query: 203 HPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVAPSSYPVITFGPFSSPTAVLKSF 262
           HPWVL +LP GE LGVLADTT +CEIDLRK+ II+ ++P+SYP+ITFGPFSSPTAVL+S 
Sbjct: 126 HPWVLVVLPTGETLGVLADTTRKCEIDLRKEGIIRIISPASYPIITFGPFSSPTAVLESL 185

Query: 263 CSAV 267
             A+
Sbjct: 186 SHAI 189

[Close]

BLAST of Sgr004546 vs. TAIR 10
Match: AT3G23640.2 (heteroglycan glucosidase 1 )

HSP 1 Score: 256.9 bits (655), Expect = 1.8e-68
Identity = 123/184 (66.85%), Postives = 142/184 (77.17%), Query Frame = 0


Query: 83  EKGATTDAISGNMIFELILEDGVFRFDCSSLDRAAAHPSFSFLKSKDRDTPISSQKLPTY 142
           +   T +  S +MIFE ILE GVFRFDCS   R AA PS SF  SKDR+ PI S  +P Y
Sbjct: 6   DSSETVEMTSTDMIFEPILEHGVFRFDCSVDHRKAAFPSVSFKNSKDREVPIVSHIVPAY 65

Query: 143 IPVFECRLGQQIVKLELPAGTSFYGTGEVSGQLERTGKRIFTWNTDAYGYGSGTTSLYQT 202
           IP   C   QQ+V  E   GTSFYGTGEVSGQLERTGKR+FTWNTDA+GYGSGTTSLYQ+
Sbjct: 66  IPTCGCLQDQQVVTFEFSPGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGSGTTSLYQS 125

Query: 203 HPWVLAILPNGEALGVLADTTLRCEIDLRKDSIIQFVAPSSYPVITFGPFSSPTAVLKSF 262
           HPWVL +LP GE LGVLADTT +CEIDLRK+ II+ ++P+SYP+ITFGPFSSPTAVL+S 
Sbjct: 126 HPWVLVVLPTGETLGVLADTTRKCEIDLRKEGIIRIISPASYPIITFGPFSSPTAVLESL 185

Query: 263 CSAV 267
             A+
Sbjct: 186 SHAI 189

The following BLAST results are available for this feature:

BLAST of Sgr004546 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145307.1	6.2e-116	81.51	uncharacterized protein LOC111014792 [Momordica charantia]	[more]
XP_038876569.1	9.3e-112	80.22	alpha-glucosidase 2 [Benincasa hispida]	[more]
XP_022923248.1	1.2e-111	79.32	uncharacterized protein LOC111430993 isoform X2 [Cucurbita moschata]	[more]
KAG6577761.1	1.2e-111	79.32	hypothetical protein SDJN03_25335, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022923247.1	1.2e-111	79.32	uncharacterized protein LOC111430993 isoform X1 [Cucurbita moschata]	[more]

BLAST of Sgr004546 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9F234	3.2e-06	32.26	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1	[more]

BLAST of Sgr004546 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CW78	3.0e-116	81.51	uncharacterized protein LOC111014792 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A6J1E5M6	5.9e-112	79.32	uncharacterized protein LOC111430993 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1E909	5.9e-112	79.32	uncharacterized protein LOC111430993 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1HNP7	7.7e-112	79.70	uncharacterized protein LOC111465248 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1HQT7	7.7e-112	79.70	uncharacterized protein LOC111465248 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of Sgr004546 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23640.1	1.8e-68	66.85	heteroglycan glucosidase 1	[more]
AT3G23640.2	1.8e-68	66.85	heteroglycan glucosidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025887	Glycoside hydrolase family 31, N-terminal domain	PFAM	PF13802	Gal_mutarotas_2	coord: 163..229 e-value: 1.8E-13 score: 50.6
None	No IPR available	GENE3D	2.60.40.1760	glycosyl hydrolase (family 31)	coord: 115..250 e-value: 1.3E-21 score: 78.9
None	No IPR available	PANTHER	PTHR22762	ALPHA-GLUCOSIDASE	coord: 77..265
None	No IPR available	PANTHER	PTHR22762:SF149	BNAA01G23630D PROTEIN	coord: 77..265
None	No IPR available	CDD	cd14752	GH31_N	coord: 145..262 e-value: 2.3722E-16 score: 71.448
IPR011013	Galactose mutarotase-like domain superfamily	SUPERFAMILY	74650	Galactose mutarotase-like	coord: 132..242

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr004546.1	Sgr004546.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0003824	catalytic activity