Sgr000896 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr000896
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	tig00000592: 44072 .. 45346 (+)
RNA-Seq Expression	Sgr000896
Synteny	Sgr000896

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGATGGAGACAAAAGGAAGGGAAGGGCTTTATGTGGGCCTGGGGGCAACCGAAACGCCACTATAAAGGAAGCTGCCTTGAAGCACCCAACACAACACACACCAACTTTTCAGAAATTCTCTGCCCTATAAAATCCCTTCTCTCTTTCTCTCCATACTTCGTGCTACTCTCTCTCTCTCTCTCTCTCTCATCGGCCATGACTGCCCAAGTGATGCCTGAGCAAGCCCTTCAGAGTATTCACCTCTTGGGAAGGGATAATGCCCCTCTGTTGCATAAAAGGTAATATTTTCATCATTTTAACTACACCCTTCATCTCTGTATCTTCCGGCGAAGCTGTTCTGCTTTCTAATGGAAGATACTGAGAATACGTGGCAGAAGTGTATTTTTGTTTTATTTTACTGTAATTTTTCTCAAGCGGTTTTGTGTTTGTTGTTGCTGGTTTAGGTTGGAGGGAAAAGTTGCAGTCGTAACGGGCGGCGCGAAGGGCATCGGAGAAGCGACGGTGAGGCTTTTCGCGAAGCACGGAGCGAGAGTAGTGATCGCCGACGTGGAGGATCTGCTCGGCGCCGCTTTGGCCGAAACGCTGTCGCCTTGTCCGGTTACTTTCGTTCACTGCGACGTTAGCTCCGAAGAAGACGTGGAGAATTTAATCAACTCGACGATTTGTCGTTACGGCCAGGTCGACATAATATTCAACAACGCCGGAGTTCTGGGGAACCAATCGAAGCGCAAGAGCATCGTCGACTTTGACCCGGACGAGTTCGACCGCGTAATGCGCGTGAACGTCAAAGGCGTGGCGTTGGGGATAAAGCACGCGGCGCGCGTCATGATTCCGAGGGGCAGCGGCTGCATCATCTCGACGGCGAGCGTGGCCGGGGTTCTGGGGGGGCTGGGGCCGCATGCGTACACGGCCTCGAAGCACGCCATTGTGGGGCTGACGAAGAACACTGCGTGCGAGCTCGGGCGCTATGGGATTCGGGTGAACTGCATTTCGCCGTTCGGAGTGGCGACGTCGATGCTGGTAAACGCCTGGAGGAGCGACGGAGACGAAGAAGACGAGTGCATGAATTTCGGGATTCCATCGGCGCAGGAAGTGGAGAAAATGGAGGAATTTGTTCGAGGTTTGGCCAATCTTAAGGGTCCGACATTGAGGCCGAAAGACATAGCAGAGGCGGCCCTCTATCTCGCCAGCGACGAATCCAAATACATCAGCGGCCACAACCTCGTCGTCGACGGTGGAATCACCACCTCAAGAAATTGTGTTGGCTTGTAA

mRNA sequence

ATGAGATGGAGACAAAAGGAAGGGAAGGGCTTTATGTGGGCCTGGGGGCAACCGAAACGCCACTATAAAGGAAGCTGCCTTGAAGCACCCAACACAACACACACCAACTTTTCAGAAATTCTCTGCCCTATAAAATCCCTTCTCTCTTTCTCTCCATACTTCGTGCTACTCTCTCTCTCTCTCTCTCTCTCATCGGCCATGACTGCCCAAGTGATGCCTGAGCAAGCCCTTCAGAGTATTCACCTCTTGGGAAGGGATAATGCCCCTCTGTTGCATAAAAGGTTGGAGGGAAAAGTTGCAGTCGTAACGGGCGGCGCGAAGGGCATCGGAGAAGCGACGGTGAGGCTTTTCGCGAAGCACGGAGCGAGAGTAGTGATCGCCGACGTGGAGGATCTGCTCGGCGCCGCTTTGGCCGAAACGCTGTCGCCTTGTCCGGTTACTTTCGTTCACTGCGACGTTAGCTCCGAAGAAGACGTGGAGAATTTAATCAACTCGACGATTTGTCGTTACGGCCAGGTCGACATAATATTCAACAACGCCGGAGTTCTGGGGAACCAATCGAAGCGCAAGAGCATCGTCGACTTTGACCCGGACGAGTTCGACCGCGTAATGCGCGTGAACGTCAAAGGCGTGGCGTTGGGGATAAAGCACGCGGCGCGCGTCATGATTCCGAGGGGCAGCGGCTGCATCATCTCGACGGCGAGCGTGGCCGGGGTTCTGGGGGGGCTGGGGCCGCATGCGTACACGGCCTCGAAGCACGCCATTGTGGGGCTGACGAAGAACACTGCGTGCGAGCTCGGGCGCTATGGGATTCGGGTGAACTGCATTTCGCCGTTCGGAGTGGCGACGTCGATGCTGGTAAACGCCTGGAGGAGCGACGGAGACGAAGAAGACGAGTGCATGAATTTCGGGATTCCATCGGCGCAGGAAGTGGAGAAAATGGAGGAATTTGTTCGAGGTTTGGCCAATCTTAAGGGTCCGACATTGAGGCCGAAAGACATAGCAGAGGCGGCCCTCTATCTCGCCAGCGACGAATCCAAATACATCAGCGGCCACAACCTCGTCGTCGACGGTGGAATCACCACCTCAAGAAATTGTGTTGGCTTGTAA

Coding sequence (CDS)

ATGAGATGGAGACAAAAGGAAGGGAAGGGCTTTATGTGGGCCTGGGGGCAACCGAAACGCCACTATAAAGGAAGCTGCCTTGAAGCACCCAACACAACACACACCAACTTTTCAGAAATTCTCTGCCCTATAAAATCCCTTCTCTCTTTCTCTCCATACTTCGTGCTACTCTCTCTCTCTCTCTCTCTCTCATCGGCCATGACTGCCCAAGTGATGCCTGAGCAAGCCCTTCAGAGTATTCACCTCTTGGGAAGGGATAATGCCCCTCTGTTGCATAAAAGGTTGGAGGGAAAAGTTGCAGTCGTAACGGGCGGCGCGAAGGGCATCGGAGAAGCGACGGTGAGGCTTTTCGCGAAGCACGGAGCGAGAGTAGTGATCGCCGACGTGGAGGATCTGCTCGGCGCCGCTTTGGCCGAAACGCTGTCGCCTTGTCCGGTTACTTTCGTTCACTGCGACGTTAGCTCCGAAGAAGACGTGGAGAATTTAATCAACTCGACGATTTGTCGTTACGGCCAGGTCGACATAATATTCAACAACGCCGGAGTTCTGGGGAACCAATCGAAGCGCAAGAGCATCGTCGACTTTGACCCGGACGAGTTCGACCGCGTAATGCGCGTGAACGTCAAAGGCGTGGCGTTGGGGATAAAGCACGCGGCGCGCGTCATGATTCCGAGGGGCAGCGGCTGCATCATCTCGACGGCGAGCGTGGCCGGGGTTCTGGGGGGGCTGGGGCCGCATGCGTACACGGCCTCGAAGCACGCCATTGTGGGGCTGACGAAGAACACTGCGTGCGAGCTCGGGCGCTATGGGATTCGGGTGAACTGCATTTCGCCGTTCGGAGTGGCGACGTCGATGCTGGTAAACGCCTGGAGGAGCGACGGAGACGAAGAAGACGAGTGCATGAATTTCGGGATTCCATCGGCGCAGGAAGTGGAGAAAATGGAGGAATTTGTTCGAGGTTTGGCCAATCTTAAGGGTCCGACATTGAGGCCGAAAGACATAGCAGAGGCGGCCCTCTATCTCGCCAGCGACGAATCCAAATACATCAGCGGCCACAACCTCGTCGTCGACGGTGGAATCACCACCTCAAGAAATTGTGTTGGCTTGTAA

Protein sequence

MRWRQKEGKGFMWAWGQPKRHYKGSCLEAPNTTHTNFSEILCPIKSLLSFSPYFVLLSLSLSLSSAMTAQVMPEQALQSIHLLGRDNAPLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALAETLSPCPVTFVHCDVSSEEDVENLINSTICRYGQVDIIFNNAGVLGNQSKRKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSDGDEEDECMNFGIPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVGL

Homology

[Close]

BLAST of Sgr000896 vs. NCBI nr
Match: XP_023528551.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562.4 bits (1448), Expect = 2.9e-156
Identity = 295/342 (86.26%), Postives = 313/342 (91.52%), Query Frame = 0


Query: 34  HTNFSEILCPIKSLLSFSPYFV-LLSLSLSLS---SAMTAQVMPEQALQS-IHLLGRDNA 93
           H   S+I  PI       P+FV  LSLSLS S    AMTAQV+PEQALQS IHLLGRDN+
Sbjct: 56  HPPTSQIPLPINPPSISLPHFVQTLSLSLSPSLSLPAMTAQVLPEQALQSNIHLLGRDNS 115

Query: 94  PLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALAETLSPCPVTF 153
           P LHKRLEGKVA+VTGGAKGIGEATVRLFAKHGA+VVIADVEDLLG ALA+TLSP PV+F
Sbjct: 116 PSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGQALADTLSPSPVSF 175

Query: 154 VHCDVSSEEDVENLINSTICRYGQVDIIFNNAGVLGNQSK-RKSIVDFDPDEFDRVMRVN 213
           VHCDVSSEEDVENLI+ST+CR+GQVDIIFNNAGVLGNQSK  KSIVDFDP+EF+RVMRVN
Sbjct: 176 VHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGNQSKTHKSIVDFDPNEFERVMRVN 235

Query: 214 VKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELG 273
           VKGVALGIKHAARVMIPR +GCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELG
Sbjct: 236 VKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELG 295

Query: 274 RYGIRVNCISPFGVATSMLVNAWRSDGDEEDECMNFGIPSAQEVEKMEEFVRGLANLKGP 333
           RYGIRVNCISPFGVATSMLVNAWR+DGDEEDECMN GIPS  EV+KMEEFVRGL+NLKGP
Sbjct: 296 RYGIRVNCISPFGVATSMLVNAWRADGDEEDECMNLGIPSVGEVDKMEEFVRGLSNLKGP 355

Query: 334 TLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVGL 370
           TLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSRNC+GL
Sbjct: 356 TLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCIGL 397

[Close]

BLAST of Sgr000896 vs. NCBI nr
Match: XP_022934318.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 555.4 bits (1430), Expect = 3.6e-154
Identity = 279/305 (91.48%), Postives = 295/305 (96.72%), Query Frame = 0


Query: 67  MTAQVMPEQALQS-IHLLGRDNAPLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGARVV 126
           MTAQV+PEQALQS IHLLGRDN+P LHKRLEGKVA+VTGGAKGIGEATVRLFAKHGA+VV
Sbjct: 1   MTAQVLPEQALQSNIHLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 127 IADVEDLLGAALAETLSPCPVTFVHCDVSSEEDVENLINSTICRYGQVDIIFNNAGVLGN 186
           IADVEDLLG ALA+TLSP PV+FVHCDVSSEEDVENLI+ST+CR+GQVDIIFNNAGVLGN
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 187 QSK-RKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLG 246
           QSK  KSIVDFDP+EF+RVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLGGLG
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 247 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSDGDEEDECMNFG 306
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+DGDEEDECMN G
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMNLG 240

Query: 307 IPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 366
           IPS  EV+KMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR
Sbjct: 241 IPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 300

Query: 367 NCVGL 370
           NC+GL
Sbjct: 301 NCIGL 305

[Close]

BLAST of Sgr000896 vs. NCBI nr
Match: XP_022983300.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita maxima])

HSP 1 Score: 550.1 bits (1416), Expect = 1.5e-152
Identity = 277/305 (90.82%), Postives = 294/305 (96.39%), Query Frame = 0


Query: 67  MTAQVMPEQALQ-SIHLLGRDNAPLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGARVV 126
           MTAQV+PEQALQ +I LLGRDN+P LHKRLEGKVA+VTGGAKGIGEATVRLFAKHGA+VV
Sbjct: 1   MTAQVLPEQALQTNILLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 127 IADVEDLLGAALAETLSPCPVTFVHCDVSSEEDVENLINSTICRYGQVDIIFNNAGVLGN 186
           IADVEDLLG ALA+TLSP PV+FVHCDVSSEEDVENLI+ST+CR+GQVDIIFNNAGVLGN
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 187 QSK-RKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLG 246
           QSK  KSIVDFDP+EF+RVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLGGLG
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 247 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSDGDEEDECMNFG 306
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+DGDEEDECMN G
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMNLG 240

Query: 307 IPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 366
           IPS  EV+KMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR
Sbjct: 241 IPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 300

Query: 367 NCVGL 370
           NC+GL
Sbjct: 301 NCIGL 305

[Close]

BLAST of Sgr000896 vs. NCBI nr
Match: XP_038905282.1 (short-chain dehydrogenase reductase 2a [Benincasa hispida])

HSP 1 Score: 540.4 bits (1391), Expect = 1.2e-149
Identity = 271/306 (88.56%), Postives = 293/306 (95.75%), Query Frame = 0


Query: 67  MTAQVMPEQALQ-SIHLLGRD-NAPLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGARV 126
           MTAQ++PEQ LQ +IHLLGRD N+P  HKRLEGKVA+VTGGAKGIGEATVRLFAKHGA+V
Sbjct: 1   MTAQLLPEQPLQTNIHLLGRDNNSPSFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKV 60

Query: 127 VIADVEDLLGAALAETLSPCPVTFVHCDVSSEEDVENLINSTICRYGQVDIIFNNAGVLG 186
           VIADVED+LG  LAETLSP PV+FVHCDVSSEEDVENL+++T+CR+GQVDIIFNNAGVLG
Sbjct: 61  VIADVEDVLGQTLAETLSPFPVSFVHCDVSSEEDVENLVSTTVCRHGQVDIIFNNAGVLG 120

Query: 187 NQSK-RKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGL 246
           +QSK RKSI+DFDPDEF+RVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLGGL
Sbjct: 121 SQSKARKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGL 180

Query: 247 GPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSDGDEEDECMNF 306
           GPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+D DEED+CMNF
Sbjct: 181 GPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVDEEDQCMNF 240

Query: 307 GIPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 366
           G+PSA EV+KMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS
Sbjct: 241 GVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300

Query: 367 RNCVGL 370
            NC+GL
Sbjct: 301 NNCIGL 306

[Close]

BLAST of Sgr000896 vs. NCBI nr
Match: XP_004137687.1 (short-chain dehydrogenase reductase 2a [Cucumis sativus] >KGN58711.1 hypothetical protein Csa_002296 [Cucumis sativus])

HSP 1 Score: 529.6 bits (1363), Expect = 2.1e-146
Identity = 265/308 (86.04%), Postives = 291/308 (94.48%), Query Frame = 0


Query: 67  MTAQVMPEQALQS-IHLLGRDNA---PLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGA 126
           MTAQV+PEQ+LQS IHLL RDN+   P  HKRLEGKVA+VTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQSLQSNIHLLVRDNSSSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 127 RVVIADVEDLLGAALAETLSPCPVTFVHCDVSSEEDVENLINSTICRYGQVDIIFNNAGV 186
           +VVIADVED+LG ALA TLSP PV+FVHCDVSSE+DVENL+++T+C +GQVDIIFNNAGV
Sbjct: 61  KVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGV 120

Query: 187 LGNQS-KRKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 246
           LGNQS   KSI+DFDPDEF+RVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 121 LGNQSNSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 247 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSDGDEEDECM 306
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+D +EE+ECM
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEEEECM 240

Query: 307 NFGIPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 366
           N+G+PSA EV+KMEEFVRGLANLKGPTL+PKDIA+AALYLASDESKYISGHNLVVDGG+T
Sbjct: 241 NYGVPSAAEVDKMEEFVRGLANLKGPTLKPKDIAQAALYLASDESKYISGHNLVVDGGVT 300

Query: 367 TSRNCVGL 370
           TSRNC+GL
Sbjct: 301 TSRNCIGL 308

[Close]

BLAST of Sgr000896 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.1e-113
Identity = 217/313 (69.33%), Postives = 252/313 (80.51%), Query Frame = 0


Query: 67  MTAQVMPEQ-ALQSIH--LLGRDNAPLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGAR 126
           M AQV+ EQ    S+H  ++   N  L  KRLEGKVA++TGGA GIG+ATV LFA+HGA 
Sbjct: 1   MPAQVIAEQTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGAT 60

Query: 127 VVIADVEDLLGAALAETLSPCP----VTFVHCDVSSEEDVENLINSTICRYGQVDIIFNN 186
           VVIADV+++ G++LA++LS       V F+ CDVS E DVENL+N T+ RYG++DI+FNN
Sbjct: 61  VVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNN 120

Query: 187 AGVLGNQSKRKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRG-SGCIISTASVAG 246
           AGVLG+Q K KSI+DFD DEFD VMRVNV+GV LG+KH AR MI RG  GCIISTASVAG
Sbjct: 121 AGVLGDQKKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVAG 180

Query: 247 VLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR--SDGDE 306
           V+GG+GPHAYTASKHAIVGLTKN ACELG+YGIRVNCISPFGVATSMLVNAWR  S GD 
Sbjct: 181 VMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGDV 240

Query: 307 EDECMNFGIPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVV 366
           ED+          +VE+MEEFVR LANLKG TLR  DIAEAALYLASDESKY++GHNLVV
Sbjct: 241 EDD----------DVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVV 300

Query: 367 DGGITTSRNCVGL 370
           DGG+TT+RNCVGL
Sbjct: 301 DGGVTTARNCVGL 303

[Close]

BLAST of Sgr000896 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.8e-101
Identity = 192/287 (66.90%), Postives = 229/287 (79.79%), Query Frame = 0


Query: 93  KRLEGKVAVVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALAETLSPCPVTFVHCD 152
           KRL+GKVA+VTGGA+GIGEA VRLFAKHGARVVIAD++D  G ALA  L P  V+FV CD
Sbjct: 51  KRLDGKVAIVTGGARGIGEAIVRLFAKHGARVVIADIDDAAGEALASALGP-QVSFVRCD 110

Query: 153 VSSEEDVENLINSTICRY-GQVDIIFNNAGVLGNQSK-RKSIVDFDPDEFDRVMRVNVKG 212
           VS E+DV   ++  + R+ G++D+  NNAGVLG Q++  +SI+ FD  EFDRV+RVN  G
Sbjct: 111 VSVEDDVRRAVDWALSRHGGRLDVYCNNAGVLGRQTRAARSILSFDAAEFDRVLRVNALG 170

Query: 213 VALGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYG 272
            ALG+KHAAR M PR +G I+S ASVA VLGGLGPHAYTASKHAIVGLTKN ACEL  +G
Sbjct: 171 AALGMKHAARAMAPRRAGSIVSVASVAAVLGGLGPHAYTASKHAIVGLTKNAACELRAHG 230

Query: 273 IRVNCISPFGVATSMLVNAWR-------SDGDEE-DECMNFGIPSAQEVEKMEEFVRGLA 332
           +RVNC+SPFGVAT ML+NAWR       +D D + D  ++  +PS QEVEKMEE VRGLA
Sbjct: 231 VRVNCVSPFGVATPMLINAWRQGHDDATADADRDLDLDLDVTVPSDQEVEKMEEVVRGLA 290

Query: 333 NLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVGL 370
            LKGPTLRP+DIAEA L+LASDE++YISGHNLVVDGG+TTSRN +GL
Sbjct: 291 TLKGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTTSRNLIGL 336

[Close]

BLAST of Sgr000896 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.3e-70
Identity = 136/275 (49.45%), Postives = 192/275 (69.82%), Query Frame = 0


Query: 93  KRLEGKVAVVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALAETLSPCPVTFVHCD 152
           +RLEGKVA++TGGA GIGE T +LF++HGA+V IADV+D LG ++ E +     T++HCD
Sbjct: 13  RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCD 72

Query: 153 VSSEEDVENLINSTICRYGQVDIIFNNAGVLGNQSKRKSIVDFDPDEFDRVMRVNVKGVA 212
           V++E+ V+N +++T+  YG++DI+F+NAG+  +   R  I+D +  +F+RV  VNV GV 
Sbjct: 73  VTNEDGVKNAVDNTVSTYGKLDIMFSNAGI--SDPNRPRIIDNEKADFERVFSVNVTGVF 132

Query: 213 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 272
           L +KHAARVMIP  SG IISTAS++  +GG   HAY  SKHA++GLT+N A ELG++GIR
Sbjct: 133 LCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIR 192

Query: 273 VNCISPFGVATSMLVNAWRSDGDEEDECMNFGIPSAQEVEKMEEFVRGLANLKGPTLRPK 332
           VNC+SPFG+ T++        G +       GI + +E E +  F     NLKGP    +
Sbjct: 193 VNCLSPFGLPTAL--------GKKFS-----GIKNEEEFENVINFA---GNLKGPKFNVE 252

Query: 333 DIAEAALYLASDESKYISGHNLVVDGGITTSRNCV 368
           D+A AALYLASDE+KY+SGHNL +DGG +   + +
Sbjct: 253 DVANAALYLASDEAKYVSGHNLFIDGGFSVCNSVI 269

[Close]

BLAST of Sgr000896 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 4.1e-68
Identity = 137/273 (50.18%), Postives = 182/273 (66.67%), Query Frame = 0


Query: 94  RLEGKVAVVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALAETLSPCP-VTFVHCD 153
           RLEGKVA+VTGGA GIGE+  RLF +HGA++ I DV+D LG  +++ L   P   + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 154 VSSEEDVENLINSTICRYGQVDIIFNNAGVLGNQSKRKSIVDFDPDEFDRVMRVNVKGVA 213
           V+ E+DV   ++ T  +YG +DI+ NNAG+ G+  K   I D D +EF +V  +NV GV 
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGD--KVIDIRDADFNEFKKVFDINVNGVF 121

Query: 214 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 273
           LG+KHAAR+MIP+  G I+S ASV+ V+ G GPH YT +KHA+VGLTK+ A ELGR+GIR
Sbjct: 122 LGMKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIR 181

Query: 274 VNCISPFGVATSMLVNAWRSDGDEEDECMNFGIPSAQEVEKMEEFVRGLANLKGPTLRPK 333
           VNC+SP+ V T + +        +ED    F             FVR  ANLKG  L P 
Sbjct: 182 VNCVSPYAVPTRLSMPYLPESEMQEDALRGF-----------LTFVRSNANLKGVDLMPN 241

Query: 334 DIAEAALYLASDESKYISGHNLVVDGGITTSRN 366
           D+AEA LYLA++ESKY+SG NLV+DGG + + +
Sbjct: 242 DVAEAVLYLATEESKYVSGLNLVIDGGFSIANH 261

[Close]

BLAST of Sgr000896 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.2e-65
Identity = 136/277 (49.10%), Postives = 179/277 (64.62%), Query Frame = 0


Query: 93  KRLEGKVAVVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALAETLSPCPVTFVHCD 152
           ++L GKVAV+TGGA GIG  T RLF KHGARVV+AD++D LGA+L   L P   ++VHCD
Sbjct: 13  RKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCD 72

Query: 153 VSSEEDVENLINSTICRYGQVDIIFNNAGVLGNQSKRKSIVDFDPDEFDRVMRVNVKGVA 212
           V++E DV   ++  + R+G++D++FNNAGV G    R S  +   ++F+RV+ VN+ G  
Sbjct: 73  VTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMS--ECTKEDFERVLAVNLVGPF 132

Query: 213 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 272
           LG KHAARVM P   G IISTAS++  + G   HAYT SKHA+VG T+N A ELGR+GIR
Sbjct: 133 LGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIR 192

Query: 273 VNCISPFGVATSMLVNAWRSDGDEEDECMNFGIPSAQEVEKMEEFVRGLANLKGP-TLRP 332
           VNC+SP GVAT +   A   D                  E +E  +   ANLKG   L+ 
Sbjct: 193 VNCVSPAGVATPLARAAMGMDD-----------------EAIEAIMANSANLKGAGALKA 252

Query: 333 KDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVG 369
            DIA AAL+LASD+ +Y+SG NL VDGG++   +  G
Sbjct: 253 DDIAAAALFLASDDGRYVSGQNLRVDGGLSVVNSSFG 270

[Close]

BLAST of Sgr000896 vs. ExPASy TrEMBL
Match: A0A6J1F7B6 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC111441518 PE=4 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.7e-154
Identity = 279/305 (91.48%), Postives = 295/305 (96.72%), Query Frame = 0


Query: 67  MTAQVMPEQALQS-IHLLGRDNAPLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGARVV 126
           MTAQV+PEQALQS IHLLGRDN+P LHKRLEGKVA+VTGGAKGIGEATVRLFAKHGA+VV
Sbjct: 1   MTAQVLPEQALQSNIHLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 127 IADVEDLLGAALAETLSPCPVTFVHCDVSSEEDVENLINSTICRYGQVDIIFNNAGVLGN 186
           IADVEDLLG ALA+TLSP PV+FVHCDVSSEEDVENLI+ST+CR+GQVDIIFNNAGVLGN
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 187 QSK-RKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLG 246
           QSK  KSIVDFDP+EF+RVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLGGLG
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 247 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSDGDEEDECMNFG 306
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+DGDEEDECMN G
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMNLG 240

Query: 307 IPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 366
           IPS  EV+KMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR
Sbjct: 241 IPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 300

Query: 367 NCVGL 370
           NC+GL
Sbjct: 301 NCIGL 305

[Close]

BLAST of Sgr000896 vs. ExPASy TrEMBL
Match: A0A6J1J5H2 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111481921 PE=4 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 7.3e-153
Identity = 277/305 (90.82%), Postives = 294/305 (96.39%), Query Frame = 0


Query: 67  MTAQVMPEQALQ-SIHLLGRDNAPLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGARVV 126
           MTAQV+PEQALQ +I LLGRDN+P LHKRLEGKVA+VTGGAKGIGEATVRLFAKHGA+VV
Sbjct: 1   MTAQVLPEQALQTNILLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 127 IADVEDLLGAALAETLSPCPVTFVHCDVSSEEDVENLINSTICRYGQVDIIFNNAGVLGN 186
           IADVEDLLG ALA+TLSP PV+FVHCDVSSEEDVENLI+ST+CR+GQVDIIFNNAGVLGN
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 187 QSK-RKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLG 246
           QSK  KSIVDFDP+EF+RVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLGGLG
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 247 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSDGDEEDECMNFG 306
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+DGDEEDECMN G
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMNLG 240

Query: 307 IPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 366
           IPS  EV+KMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR
Sbjct: 241 IPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 300

Query: 367 NCVGL 370
           NC+GL
Sbjct: 301 NCIGL 305

[Close]

BLAST of Sgr000896 vs. ExPASy TrEMBL
Match: A0A0A0LF57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 1.0e-146
Identity = 265/308 (86.04%), Postives = 291/308 (94.48%), Query Frame = 0


Query: 67  MTAQVMPEQALQS-IHLLGRDNA---PLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGA 126
           MTAQV+PEQ+LQS IHLL RDN+   P  HKRLEGKVA+VTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQSLQSNIHLLVRDNSSSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 127 RVVIADVEDLLGAALAETLSPCPVTFVHCDVSSEEDVENLINSTICRYGQVDIIFNNAGV 186
           +VVIADVED+LG ALA TLSP PV+FVHCDVSSE+DVENL+++T+C +GQVDIIFNNAGV
Sbjct: 61  KVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGV 120

Query: 187 LGNQS-KRKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 246
           LGNQS   KSI+DFDPDEF+RVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 121 LGNQSNSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 247 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSDGDEEDECM 306
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+D +EE+ECM
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEEEECM 240

Query: 307 NFGIPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 366
           N+G+PSA EV+KMEEFVRGLANLKGPTL+PKDIA+AALYLASDESKYISGHNLVVDGG+T
Sbjct: 241 NYGVPSAAEVDKMEEFVRGLANLKGPTLKPKDIAQAALYLASDESKYISGHNLVVDGGVT 300

Query: 367 TSRNCVGL 370
           TSRNC+GL
Sbjct: 301 TSRNCIGL 308

[Close]

BLAST of Sgr000896 vs. ExPASy TrEMBL
Match: A0A6J1I659 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111470263 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 3.0e-146
Identity = 270/305 (88.52%), Postives = 286/305 (93.77%), Query Frame = 0


Query: 67  MTAQVMPEQALQS-IHLLGRDNAPLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGARVV 126
           MTAQV+PEQALQS IHLL RD+    +KRLEGKVA+VTGGAKGIGEATVRLFAKHGA+VV
Sbjct: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 127 IADVEDLLGAALAETLSPCPVTFVHCDVSSEEDVENLINSTICRYGQVDIIFNNAGVLGN 186
           IADVEDLLG ALA+TLSP PVTFVHCDVS EED+ENLINST+CR+GQVDI+FNNAGVLGN
Sbjct: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 187 QSK-RKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLG 246
           QSK RKSIVDFDPDEF+RVM VNVKGVALGIKHAARVMIPR +GCIISTASVAGV GG+G
Sbjct: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 247 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSDGDEEDECMNFG 306
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR DG   DECMNFG
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDG---DECMNFG 240

Query: 307 IPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 366
           IPS  EV+KMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKY+SGHNLVVDGGITTSR
Sbjct: 241 IPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSR 300

Query: 367 NCVGL 370
           NC+GL
Sbjct: 301 NCIGL 302

[Close]

BLAST of Sgr000896 vs. ExPASy TrEMBL
Match: A0A6J1G6Z3 (short-chain dehydrogenase reductase 2a OS=Cucurbita moschata OX=3662 GN=LOC111451343 PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 1.1e-145
Identity = 269/305 (88.20%), Postives = 285/305 (93.44%), Query Frame = 0


Query: 67  MTAQVMPEQALQS-IHLLGRDNAPLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGARVV 126
           MTAQV+PEQALQS IHLL RD     +KRLEGKVA+VTGGAKGIGEATVRLFAKHGA+VV
Sbjct: 1   MTAQVLPEQALQSNIHLLQRDTPAPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 127 IADVEDLLGAALAETLSPCPVTFVHCDVSSEEDVENLINSTICRYGQVDIIFNNAGVLGN 186
           IADVEDLLG ALA+TLSP PV+FVHCDVS EED+ENLINST+CR+GQVDI+FNNAGVLGN
Sbjct: 61  IADVEDLLGEALADTLSPNPVSFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 187 QSK-RKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLG 246
           QSK RKSIVDFDPDEF+RVM VNVKGVALGIKHAARVMIPR +GCIISTASVAGV GG+G
Sbjct: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 247 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSDGDEEDECMNFG 306
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR DG   DECMNFG
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDG---DECMNFG 240

Query: 307 IPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 366
           IPS  EV+KMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKY+SGHNLVVDGGITTSR
Sbjct: 241 IPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSR 300

Query: 367 NCVGL 370
           NC+GL
Sbjct: 301 NCIGL 302

[Close]

BLAST of Sgr000896 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 411.4 bits (1056), Expect = 7.8e-115
Identity = 217/313 (69.33%), Postives = 252/313 (80.51%), Query Frame = 0


Query: 67  MTAQVMPEQ-ALQSIH--LLGRDNAPLLHKRLEGKVAVVTGGAKGIGEATVRLFAKHGAR 126
           M AQV+ EQ    S+H  ++   N  L  KRLEGKVA++TGGA GIG+ATV LFA+HGA 
Sbjct: 1   MPAQVIAEQTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGAT 60

Query: 127 VVIADVEDLLGAALAETLSPCP----VTFVHCDVSSEEDVENLINSTICRYGQVDIIFNN 186
           VVIADV+++ G++LA++LS       V F+ CDVS E DVENL+N T+ RYG++DI+FNN
Sbjct: 61  VVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNN 120

Query: 187 AGVLGNQSKRKSIVDFDPDEFDRVMRVNVKGVALGIKHAARVMIPRG-SGCIISTASVAG 246
           AGVLG+Q K KSI+DFD DEFD VMRVNV+GV LG+KH AR MI RG  GCIISTASVAG
Sbjct: 121 AGVLGDQKKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVAG 180

Query: 247 VLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR--SDGDE 306
           V+GG+GPHAYTASKHAIVGLTKN ACELG+YGIRVNCISPFGVATSMLVNAWR  S GD 
Sbjct: 181 VMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGDV 240

Query: 307 EDECMNFGIPSAQEVEKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVV 366
           ED+          +VE+MEEFVR LANLKG TLR  DIAEAALYLASDESKY++GHNLVV
Sbjct: 241 EDD----------DVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVV 300

Query: 367 DGGITTSRNCVGL 370
           DGG+TT+RNCVGL
Sbjct: 301 DGGVTTARNCVGL 303

[Close]

BLAST of Sgr000896 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 250.0 bits (637), Expect = 3.0e-66
Identity = 132/269 (49.07%), Postives = 178/269 (66.17%), Query Frame = 0


Query: 93  KRLEGKVAVVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALAETLSPCPVTFVHCD 152
           K+LEGKVA++TGGA G+G+AT   F +HGARVVIAD++   G   A+ L      FV CD
Sbjct: 39  KKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELG-SEAEFVRCD 98

Query: 153 VSSEEDVENLINSTICRYGQVDIIFNNAGVLGNQSKRKSIVDFDPDEFDRVMRVNVKGVA 212
           V+ E D+   +  T+ RYG++D+++NNAG++G  +   SI   D  EF+RVMR+NV GV 
Sbjct: 99  VTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTP-ASISQLDMTEFERVMRINVFGVV 158

Query: 213 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 272
            GIKHAA+ MIP  SGCI+ T+SVAGV GGL PH+YT SK    G+ K+ A EL  +G+R
Sbjct: 159 SGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVR 218

Query: 273 VNCISPFGVATSMLVNAWRSDGDEEDECMNFGIPSAQEVEKMEEFVRGLANLKGPTLRPK 332
           +NCISP  VAT + ++            +    P   E EK+ E V+G+  LKG      
Sbjct: 219 INCISPGTVATPLTLS-----------YLQKVFPKVSE-EKLRETVKGMGELKGAECEEA 278

Query: 333 DIAEAALYLASDESKYISGHNLVVDGGIT 362
           D+A+AALYLAS++ KY++GHNLVVDGG+T
Sbjct: 279 DVAKAALYLASNDGKYVTGHNLVVDGGMT 293

[Close]

BLAST of Sgr000896 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.6e-64
Identity = 129/269 (47.96%), Postives = 180/269 (66.91%), Query Frame = 0


Query: 94  RLEGKVAVVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALAETLSPCPVTFVHCDV 153
           RL+GK+A++TGGA GIG   VRLF  HGA+VVI D+++ LG  LA ++     +F  C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 154 SSEEDVENLINSTICRYGQVDIIFNNAGVLGNQSKRKSIVDFDPDEFDRVMRVNVKGVAL 213
           + E DVEN +  T+ ++G++D++F+NAGVL       S++D D + FDR M VNV+G A 
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVL---EAFGSVLDLDLEAFDRTMAVNVRGAAA 124

Query: 214 GIKHAARVMIPRGS-GCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 273
            IKHAAR M+  G+ G I+ T S+A  +GG GPH+YTASKHA++GL ++    LG+YGIR
Sbjct: 125 FIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIR 184

Query: 274 VNCISPFGVATSMLVNAWRSDGDEEDECMNFGIPSAQEVEKMEEFVRGLANLKGPTLRPK 333
           VN ++P+GVAT M  +A+  +                 V+ +EE+   L NLKG  L+ +
Sbjct: 185 VNGVAPYGVATGM-TSAYNEEA----------------VKMLEEYGEALGNLKGVVLKAR 244

Query: 334 DIAEAALYLASDESKYISGHNLVVDGGIT 362
            IAEAAL+LASD+S YISG NLVVDGG +
Sbjct: 245 HIAEAALFLASDDSVYISGQNLVVDGGFS 253

[Close]

BLAST of Sgr000896 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 243.0 bits (619), Expect = 3.7e-64
Identity = 129/272 (47.43%), Postives = 181/272 (66.54%), Query Frame = 0


Query: 91  LHKRLEGKVAVVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALAETLSPCPVTFVH 150
           L + L+GK+A++TGGA GIG   VRLF  HGA+VVI D+++ LG  LA ++     +F  
Sbjct: 40  LRQVLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYR 99

Query: 151 CDVSSEEDVENLINSTICRYGQVDIIFNNAGVLGNQSKRKSIVDFDPDEFDRVMRVNVKG 210
           C+V+ E DVEN +  T+ ++G++D++F+NAGVL       S++D D + FDR M VNV+G
Sbjct: 100 CNVTDETDVENAVKFTVEKHGKLDVLFSNAGVL---EAFGSVLDLDLEAFDRTMAVNVRG 159

Query: 211 VALGIKHAARVMIPRGS-GCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRY 270
            A  IKHAAR M+  G+ G I+ T S+A  +GG GPH+YTASKHA++GL ++    LG+Y
Sbjct: 160 AAAFIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQY 219

Query: 271 GIRVNCISPFGVATSMLVNAWRSDGDEEDECMNFGIPSAQEVEKMEEFVRGLANLKGPTL 330
           GIRVN ++P+GVAT M  +A+  +                 V+ +EE+   L NLKG  L
Sbjct: 220 GIRVNGVAPYGVATGM-TSAYNEEA----------------VKMLEEYGEALGNLKGVVL 279

Query: 331 RPKDIAEAALYLASDESKYISGHNLVVDGGIT 362
           + + IAEAAL+LASD+S YISG NLVVDGG +
Sbjct: 280 KARHIAEAALFLASDDSVYISGQNLVVDGGFS 291

[Close]

BLAST of Sgr000896 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 239.6 bits (610), Expect = 4.0e-63
Identity = 123/268 (45.90%), Postives = 176/268 (65.67%), Query Frame = 0


Query: 93  KRLEGKVAVVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALAETLSPCPVTFVHCD 152
           KRL+GK+ ++TGGA GIG  +VRLF +HGARVVI DV+D LG  +A ++     ++ HCD
Sbjct: 4   KRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCD 63

Query: 153 VSSEEDVENLINSTICRYGQVDIIFNNAGVLGNQSKRKSIVDFDPDEFDRVMRVNVKGVA 212
           V++E +VEN +  T+ +YG++D++F+NAGV+       SI+D + +E DR + +N++G A
Sbjct: 64  VTNETEVENAVKFTVEKYGKLDVLFSNAGVI---EPFVSILDLNLNELDRTIAINLRGTA 123

Query: 213 LGIKHAARVMIPRG-SGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGI 272
             IKHAAR M+ +G  G I+ T SVA  + G  PH YT SKH ++GL K+ +  LG+YGI
Sbjct: 124 AFIKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGI 183

Query: 273 RVNCISPFGVATSMLVNAWRSDGDEEDECMNFGIPSAQEVEKMEEFVRGLANLKGPTLRP 332
           RVN ++PFGVAT ++ N ++                  E   +E+     ANLKG  L+ 
Sbjct: 184 RVNGVAPFGVATPLVCNGFK-----------------MEPNVVEQNTSASANLKGIVLKA 243

Query: 333 KDIAEAALYLASDESKYISGHNLVVDGG 360
           + +AEAAL+LASDES Y+SG NL VDGG
Sbjct: 244 RHVAEAALFLASDESAYVSGQNLAVDGG 251

The following BLAST results are available for this feature:

BLAST of Sgr000896 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023528551.1	2.9e-156	86.26	short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo]	[more]
XP_022934318.1	3.6e-154	91.48	short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 S...	[more]
XP_022983300.1	1.5e-152	90.82	short-chain dehydrogenase reductase 2a-like [Cucurbita maxima]	[more]
XP_038905282.1	1.2e-149	88.56	short-chain dehydrogenase reductase 2a [Benincasa hispida]	[more]
XP_004137687.1	2.1e-146	86.04	short-chain dehydrogenase reductase 2a [Cucumis sativus] >KGN58711.1 hypothetica...	[more]

BLAST of Sgr000896 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCU0	1.1e-113	69.33	Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ...	[more]
P50160	2.8e-101	66.90	Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1	[more]
Q94KL7	3.3e-70	49.45	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
F1SWA0	4.1e-68	50.18	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]
Q7FAE1	3.2e-65	49.10	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]

BLAST of Sgr000896 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F7B6	1.7e-154	91.48	short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1J5H2	7.3e-153	90.82	short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A0A0LF57	1.0e-146	86.04	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1	[more]
A0A6J1I659	3.0e-146	88.52	short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1G6Z3	1.1e-145	88.20	short-chain dehydrogenase reductase 2a OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]

BLAST of Sgr000896 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51680.1	7.8e-115	69.33	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	3.0e-66	49.07	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.2	1.6e-64	47.96	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.1	3.7e-64	47.43	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	4.0e-63	45.90	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 99..116 score: 50.79 coord: 324..344 score: 32.49 coord: 248..267 score: 44.24 coord: 269..286 score: 41.2 coord: 171..182 score: 51.1 coord: 222..238 score: 35.38
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 171..182 score: 47.86 coord: 228..236 score: 45.22 coord: 248..267 score: 38.49
None	No IPR available	GENE3D	3.40.50.720		coord: 88..367 e-value: 5.2E-81 score: 273.6
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 104..361 e-value: 4.6E-61 score: 206.4
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 94..279 e-value: 6.7E-9 score: 33.1
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 102..297 e-value: 2.0E-17 score: 61.2
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 67..369
None	No IPR available	PANTHER	PTHR43180:SF58	SHORT-CHAIN DEHYDROGENASE REDUCTASE 2A-LIKE	coord: 67..369
None	No IPR available	CDD	cd05326	secoisolariciresinol-DH_like_SDR_c	coord: 94..361 e-value: 4.89873E-132 score: 376.026
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 96..361

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr000896.1	Sgr000896.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004316	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
molecular_function	GO:0016491	oxidoreductase activity