Sed0027527 (gene) Chayote v1

BLAST of Sed0027527 vs. NCBI nr
Match: XP_023517669.1 (aspartic proteinase-like protein 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 917.9 bits (2371), Expect = 3.9e-263
Identity = 461/530 (86.98%), Postives = 497/530 (93.77%), Query Frame = 0

Query: 2   SLLLLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEY 61
           +L+L+L+MM  +S   A S I  +SR+ HRFS+EMKALRVS+S N+S+RVSWPEKGSMEY
Sbjct: 5   NLVLVLLMM--ISAHQAMS-IAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSMEY 64

Query: 62  FQELLSRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALD 121
           +QEL+S D  R+ +KLGS+FQLLFPS+GS TIALGNDFGWLHY WIDIGTPSVSFLVALD
Sbjct: 65  YQELVSGDFQRQKMKLGSQFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALD 124

Query: 122 AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSP 181
           AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPS SS+SKHISCSHNLC+SGQSCQSP
Sbjct: 125 AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSSSSTSKHISCSHNLCDSGQSCQSP 184

Query: 182 KQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGV 241
           KQSCPYVIDY TENTSSSGLLIQD+LHLSSGCE SSNCTIQAPV+LGCGMKQSGGYLSGV
Sbjct: 185 KQSCPYVIDYLTENTSSSGLLIQDVLHLSSGCENSSNCTIQAPVVLGCGMKQSGGYLSGV 244

Query: 242 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLD 301
           APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFV LD
Sbjct: 245 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVLLD 304

Query: 302 GKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSV 361
           GKYEAYIVGVEACCIGNSCL+QTSFKALIDSGTSFTYLPEEVYEN+ MEF+++LNTTS+V
Sbjct: 305 GKYEAYIVGVEACCIGNSCLEQTSFKALIDSGTSFTYLPEEVYENVVMEFDKRLNTTSTV 364

Query: 362 TFKGYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPAD 421
           TFKGYPWKYCYKIS+DAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILP D
Sbjct: 365 TFKGYPWKYCYKISADAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPTD 424

Query: 422 GDIGILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLAPAKETPPNPLPASEQQ 481
           GDIGILGQNYMTGYRMVFDRENLKL WSRA+C+DLSN+K+MPLAP+KETPPNPLPA+EQQ
Sbjct: 425 GDIGILGQNYMTGYRMVFDRENLKLSWSRANCQDLSNEKKMPLAPSKETPPNPLPANEQQ 484

Query: 482 RVPGGRAVAPAVAGRAPAKPSAATPS-IPSSF-TTRLPHLLLLVLYLVSS 528
            V GG AVAPAVAGRAP+KPSAATP  IPS F T RL HLLLLVLYLVS+
Sbjct: 485 SVSGGHAVAPAVAGRAPSKPSAATPRFIPSCFYTVRLLHLLLLVLYLVST 531

BLAST of Sed0027527 vs. NCBI nr
Match: XP_023003199.1 (aspartic proteinase-like protein 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 916.8 bits (2368), Expect = 8.8e-263
Identity = 461/532 (86.65%), Postives = 496/532 (93.23%), Query Frame = 0

Query: 2   SLLLLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEY 61
           +L+L+L+MM  +S   A S I  +SR+ HRFS+EMKALRVS+S N+S+RVSWPEKGSMEY
Sbjct: 5   NLVLVLLMM--ISAHQALS-IAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSMEY 64

Query: 62  FQELLSRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALD 121
           +QEL+S D  R+ +KLGSRFQLLFPS+GS TIALGNDFGWLHY WIDIGTPSVSFLVALD
Sbjct: 65  YQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALD 124

Query: 122 AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSP 181
           AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPS SS+SKH+SCSHNLCESGQSCQSP
Sbjct: 125 AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSSSSTSKHLSCSHNLCESGQSCQSP 184

Query: 182 KQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGV 241
           KQSCPYVIDY TENTSSSGLLIQD+LHLSSGCE SSNCTIQAPV+LGCGMKQSGGYLSGV
Sbjct: 185 KQSCPYVIDYLTENTSSSGLLIQDVLHLSSGCENSSNCTIQAPVVLGCGMKQSGGYLSGV 244

Query: 242 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLD 301
           APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFV LD
Sbjct: 245 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVLLD 304

Query: 302 GKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSV 361
           GKYEAYIVGVEACCIGNSCL+QTSFKALIDSGTSFTYLPEEVYEN+ MEF+++LNTTS+V
Sbjct: 305 GKYEAYIVGVEACCIGNSCLEQTSFKALIDSGTSFTYLPEEVYENVVMEFDKRLNTTSTV 364

Query: 362 TFKGYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPAD 421
           TFKGYPWKYCYKIS+DAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILP D
Sbjct: 365 TFKGYPWKYCYKISADAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPTD 424

Query: 422 GDIGILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLAPAKETPPNPLPASEQQ 481
           GDIGILGQNYMTGYRMVFDRENLKL WSRA+C+DLSN+K+MPLAP+KETPPN LPA+EQQ
Sbjct: 425 GDIGILGQNYMTGYRMVFDRENLKLSWSRANCQDLSNEKKMPLAPSKETPPNSLPANEQQ 484

Query: 482 RVPGGRAVAPAVAGRAPAKPSAATPS-IPSSF-TTRLPHLLLLVLYLVSSSC 530
            V GG AVAPAVAGRAP+KPSAATP  IPS F T RL HLLLLVLYL S+ C
Sbjct: 485 SVSGGHAVAPAVAGRAPSKPSAATPCFIPSCFYTVRLLHLLLLVLYLGSTCC 533

BLAST of Sed0027527 vs. NCBI nr
Match: XP_022963037.1 (aspartic proteinase-like protein 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 915.2 bits (2364), Expect = 2.6e-262
Identity = 459/530 (86.60%), Postives = 495/530 (93.40%), Query Frame = 0

Query: 2   SLLLLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEY 61
           +L+L+L+MM  +S   A S I  +SR+ HRFS+EMKALRVS+S N+S+RVSWPEKGSMEY
Sbjct: 5   NLVLVLLMM--ISAHQAMS-IAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSMEY 64

Query: 62  FQELLSRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALD 121
           +QEL+S D  R+ +KLGSRFQLLFPS+GS TIALGNDFGWLHY WIDIGTPSVSFLVALD
Sbjct: 65  YQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALD 124

Query: 122 AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSP 181
           AGSDLLW+PCDCIQCAPLSASYYGSLDKDLNEYRPS SS+SKHISCSHNLCESGQSCQSP
Sbjct: 125 AGSDLLWIPCDCIQCAPLSASYYGSLDKDLNEYRPSSSSTSKHISCSHNLCESGQSCQSP 184

Query: 182 KQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGV 241
           KQSCPYVIDY TENTSSSGLLIQD+LHLSSGCE SSNCTIQAPV+LGCGMKQSGGYLSGV
Sbjct: 185 KQSCPYVIDYLTENTSSSGLLIQDVLHLSSGCENSSNCTIQAPVVLGCGMKQSGGYLSGV 244

Query: 242 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLD 301
           APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFV LD
Sbjct: 245 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVLLD 304

Query: 302 GKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSV 361
           GKYEAYIVGVEACCIGNSCL+QTSFKALIDSGTSFTYLPEEVYEN+ MEF+++LNTTS+V
Sbjct: 305 GKYEAYIVGVEACCIGNSCLEQTSFKALIDSGTSFTYLPEEVYENVVMEFDKRLNTTSTV 364

Query: 362 TFKGYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPAD 421
           TFKGYPWKYCYKIS+DAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFA+LP D
Sbjct: 365 TFKGYPWKYCYKISADAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAVLPTD 424

Query: 422 GDIGILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLAPAKETPPNPLPASEQQ 481
           GDIGILGQNYMTGYRMVFDRENLKL WSRA+C+DLSN+K+MPLAP+KETPPNPLPA+EQQ
Sbjct: 425 GDIGILGQNYMTGYRMVFDRENLKLSWSRANCQDLSNEKKMPLAPSKETPPNPLPANEQQ 484

Query: 482 RVPGGRAVAPAVAGRAPAKPSAATPS-IPSSF-TTRLPHLLLLVLYLVSS 528
            V  G AVAPAVAGRAP+KPSAATP  IPS F T RL HLLLLV YLVS+
Sbjct: 485 SVSEGHAVAPAVAGRAPSKPSAATPCFIPSCFYTVRLLHLLLLVFYLVST 531

BLAST of Sed0027527 vs. NCBI nr
Match: KAG6594861.1 (Aspartic proteinase-like protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 908.7 bits (2347), Expect = 2.4e-260
Identity = 456/523 (87.19%), Postives = 489/523 (93.50%), Query Frame = 0

Query: 2   SLLLLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEY 61
           +L+L+L+MM  +S   A S I  +SR+ HRFS+EMKALRVS+S N+S+RVSWPEKGSMEY
Sbjct: 5   NLVLVLLMM--ISAHQAMS-IAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSMEY 64

Query: 62  FQELLSRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALD 121
           +QEL+S D  R+ +KLGSRFQLLFPS+GS TIALGNDFGWLHY WIDIGTPSVSFLVALD
Sbjct: 65  YQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALD 124

Query: 122 AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSP 181
           AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPS SS+SKHISCSHNLCESGQSCQSP
Sbjct: 125 AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSSSSTSKHISCSHNLCESGQSCQSP 184

Query: 182 KQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGV 241
           KQSCPYVIDY TENTSSSGLLIQD+LHLSSGCE SSNCTIQAPV+LGCGMKQSGGYLSGV
Sbjct: 185 KQSCPYVIDYLTENTSSSGLLIQDVLHLSSGCENSSNCTIQAPVVLGCGMKQSGGYLSGV 244

Query: 242 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLD 301
           APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFV LD
Sbjct: 245 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVLLD 304

Query: 302 GKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSV 361
           GKYEAYIVGVEACCIGNSCL+QTSFKALIDSGTSFTYLPEEVYEN+ MEF+++LNTTS+V
Sbjct: 305 GKYEAYIVGVEACCIGNSCLEQTSFKALIDSGTSFTYLPEEVYENVVMEFDKRLNTTSTV 364

Query: 362 TFKGYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPAD 421
           TFKGYPWKYCYKIS+DAMPKVPSV LLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILP D
Sbjct: 365 TFKGYPWKYCYKISADAMPKVPSVKLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPTD 424

Query: 422 GDIGILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLAPAKETPPNPLPASEQQ 481
           GDIGILGQNYMTGYRMVFDRENLKL WSRA+C+DLSN+K+MPLAP+KETPPNPLPA+EQQ
Sbjct: 425 GDIGILGQNYMTGYRMVFDRENLKLSWSRANCQDLSNEKKMPLAPSKETPPNPLPANEQQ 484

Query: 482 RVPGGRAVAPAVAGRAPAKPSAATPS-IPSSF-TTRLPHLLLL 521
            V GG AVAPAVAGRAP+KPSAATP  IPS F T RL HLLLL
Sbjct: 485 SVSGGHAVAPAVAGRAPSKPSAATPCFIPSCFYTVRLLHLLLL 524

BLAST of Sed0027527 vs. NCBI nr
Match: XP_022950779.1 (aspartic proteinase-like protein 1 isoform X1 [Cucurbita moschata] >XP_022950781.1 aspartic proteinase-like protein 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 903.7 bits (2334), Expect = 7.7e-259
Identity = 452/527 (85.77%), Postives = 489/527 (92.79%), Query Frame = 0

Query: 5   LLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEYFQE 64
           L+L+++  ++V  A S IT +SRL HRFS++MKALRVS S  + +R SWPEKGSMEY+QE
Sbjct: 6   LILLLLMVIAVHQAVS-ITFTSRLLHRFSEDMKALRVSGS-TTGVRASWPEKGSMEYYQE 65

Query: 65  LLSRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALDAGS 124
           L+S D  R+ +KLGSRFQ LFPS+GS TI LGNDFGWLHYTWIDIGTPSVSFLVALDAGS
Sbjct: 66  LVSGDFQRQKMKLGSRFQWLFPSEGSKTIELGNDFGWLHYTWIDIGTPSVSFLVALDAGS 125

Query: 125 DLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSPKQS 184
           DLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPS+SS+SKHISCSHNLC+SGQSCQSPKQS
Sbjct: 126 DLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSKSSTSKHISCSHNLCDSGQSCQSPKQS 185

Query: 185 CPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGVAPD 244
           CPYVIDYTTENTSSSGLLIQD+LHLSSGCE SSNCTIQAPVILGCGMKQSGGYLSGVAPD
Sbjct: 186 CPYVIDYTTENTSSSGLLIQDVLHLSSGCENSSNCTIQAPVILGCGMKQSGGYLSGVAPD 245

Query: 245 GLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLDGKY 304
           GLFGLGLGEISVLSSLAKEGLV NSFSLCFNEDGSGRIFFGDEGPASQQMTSFV LD KY
Sbjct: 246 GLFGLGLGEISVLSSLAKEGLVPNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVPLDEKY 305

Query: 305 EAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSVTFK 364
           EAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEE YENI MEF+++LN TS+V+FK
Sbjct: 306 EAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEAYENIVMEFDKRLNVTSTVSFK 365

Query: 365 GYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPADGDI 424
           GYPWKYCYKIS+DAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCF+ILPADGDI
Sbjct: 366 GYPWKYCYKISTDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFSILPADGDI 425

Query: 425 GILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLAPAKETPPNPLPASEQQRVP 484
           GILGQNYMTGYRMVFDR+NLKL WSRA+C+DLSN KEMP+APAKETPPNPLPA+EQQ  P
Sbjct: 426 GILGQNYMTGYRMVFDRDNLKLGWSRANCQDLSNDKEMPIAPAKETPPNPLPANEQQSAP 485

Query: 485 GGRAVAPAVAGRAPAKPSAATPSI-PSSF-TTRLPHLLLLVLYLVSS 528
           GG AVAPA+AGRAP+KPSAA P I PSSF + RLPHL+LLVL LVS+
Sbjct: 486 GGHAVAPAIAGRAPSKPSAAAPCIMPSSFYSIRLPHLILLVLCLVST 530

BLAST of Sed0027527 vs. ExPASy Swiss-Prot
Match: Q9LX20 (Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 PE=2 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 3.1e-154
Identity = 293/529 (55.39%), Postives = 374/529 (70.70%), Query Frame = 0

Query: 9   MMFTVSVRPAESTIT--LSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEYFQELL 68
           ++F V     E T+    SSRL HRFSDE +A   + S++ SL    P K S+EY++ L 
Sbjct: 8   LLFCVLFLATEETLASLFSSRLIHRFSDEGRASIKTPSSSDSL----PNKQSLEYYRLLA 67

Query: 69  SRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALDAGSDL 128
             D  R+ + LG++ Q L PS+GS TI+ GNDFGWLHYTWIDIGTPSVSFLVALD GS+L
Sbjct: 68  ESDFRRQRMNLGAKVQSLVPSEGSKTISSGNDFGWLHYTWIDIGTPSVSFLVALDTGSNL 127

Query: 129 LWVPCDCIQCAPLSASYYGSL-DKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSPKQSC 188
           LW+PC+C+QCAPL+++YY SL  KDLNEY PS SS+SK   CSH LC+S   C+SPK+ C
Sbjct: 128 LWIPCNCVQCAPLTSTYYSSLATKDLNEYNPSSSSTSKVFLCSHKLCDSASDCESPKEQC 187

Query: 189 PYVIDYTTENTSSSGLLIQDILHLSSGCEK---SSNCTIQAPVILGCGMKQSGGYLSGVA 248
           PY ++Y + NTSSSGLL++DILHL+        + + +++A V++GCG KQSG YL GVA
Sbjct: 188 PYTVNYLSGNTSSSGLLVEDILHLTYNTNNRLMNGSSSVKARVVIGCGKKQSGDYLDGVA 247

Query: 249 PDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLD- 308
           PDGL GLG  EISV S L+K GL++NSFSLCF+E+ SGRI+FGD GP+ QQ T F+ LD 
Sbjct: 248 PDGLMGLGPAEISVPSFLSKAGLMRNSFSLCFDEEDSGRIYFGDMGPSIQQSTPFLQLDN 307

Query: 309 GKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSV 368
            KY  YIVGVEACCIGNSCLKQTSF   IDSG SFTYLPEE+Y  +A+E +R +N TS  
Sbjct: 308 NKYSGYIVGVEACCIGNSCLKQTSFTTFIDSGQSFTYLPEEIYRKVALEIDRHINATSK- 367

Query: 369 TFKGYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPAD 428
            F+G  W+YCY+  S A PKVP++ L F  NN+FV+H P+F     QGL  FC  I P+ 
Sbjct: 368 NFEGVSWEYCYE--SSAEPKVPAIKLKFSHNNTFVIHKPLFVFQQSQGLVQFCLPISPSG 427

Query: 429 GD-IGILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLA-PAKETPPNPLPASE 488
            + IG +GQNYM GYRMVFDREN+KL WS + C++  ++ E P A P   + PNPLP  E
Sbjct: 428 QEGIGSIGQNYMRGYRMVFDRENMKLGWSPSKCQE--DKIEPPQASPGSTSSPNPLPTDE 487

Query: 489 QQRVPGGRAVAPAVAGRAPAKPSAATPSIPSSFTTRLPHLLLLVLYLVS 527
           QQ   GG AV+PA+AG+ P+K  +++ S   S   RL + LLL+ +L S
Sbjct: 488 QQS-RGGHAVSPAIAGKTPSKTPSSSSSYSFSSIMRLFNSLLLLHWLAS 526

BLAST of Sed0027527 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.2e-73
Identity = 185/503 (36.78%), Postives = 262/503 (52.09%), Query Frame = 0

Query: 30  HRFSDEMKALRVSKSANSSLRVSWPEKGSMEYFQELLSRDR--KNLKLGSRFQLLFPSQG 89
           HRFSD++  +              P + S +Y++ +  RDR  +  +L +  Q L     
Sbjct: 39  HRFSDQVVGVLPGDGL--------PNRDSSKYYRVMAHRDRLIRGRRLANEDQSLVTFSD 98

Query: 90  SNTIALGNDFGWLHYTWIDIGTPSVSFLVALDAGSDLLWVPCDCIQCA-PLSASYYGSLD 149
            N     +  G+LHY  + +GTPS  F+VALD GSDL W+PCDC  C   L A   G   
Sbjct: 99  GNETVRVDALGFLHYANVTVGTPSDWFMVALDTGSDLFWLPCDCTNCVRELKAP--GGSS 158

Query: 150 KDLNEYRPSRSSSSKHISCSHNLCESGQSCQSPKQSCPYVIDYTTENTSSSGLLIQDILH 209
            DLN Y P+ SS+S  + C+  LC  G  C SP+  CPY I Y +  TSS+G+L++D+LH
Sbjct: 159 LDLNIYSPNASSTSTKVPCNSTLCTRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLH 218

Query: 210 LSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGVAPDGLFGLGLGEISVLSSLAKEGLVQN 269
           L S  +KSS   I A V  GCG  Q+G +  G AP+GLFGLGL +ISV S LAKEG+  N
Sbjct: 219 LVSN-DKSSK-AIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAAN 278

Query: 270 SFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLDGKYEAYIVGVEACCIGNSCLKQTSFKA 329
           SFS+CF  DG+GRI FGD+G   Q+ T  +++   +  Y + V    +G +      F A
Sbjct: 279 SFSMCFGNDGAGRISFGDKGSVDQRETP-LNIRQPHPTYNITVTKISVGGN-TGDLEFDA 338

Query: 330 LIDSGTSFTYLPEEVYENIAMEFNR-KLNTTSSVTFKGYPWKYCYKISSDAMP-KVPSVT 389
           + DSGTSFTYL +  Y  I+  FN   L+     T    P++YCY +S +    + P+V 
Sbjct: 339 VFDSGTSFTYLTDAAYTLISESFNSLALDKRYQTTDSELPFEYCYALSPNKDSFQYPAVN 398

Query: 390 LLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPADGDIGILGQNYMTGYRMVFDRENLKL 449
           L     +S+ V+ P+  +   +    +C AI+  + DI I+GQN+MTGYR+VFDRE L L
Sbjct: 399 LTMKGGSSYPVYHPLV-VIPMKDTDVYCLAIMKIE-DISIIGQNFMTGYRVVFDREKLIL 458

Query: 450 RWSRASCEDLSNQKEMPLAPAKETPPNPLPA--SEQQRVPGGRAVAPAVAGRAPAKPSAA 509
            W  + C               ET    LP+  S     P   +  P        +P+ +
Sbjct: 459 GWKESDCY------------TGETSARTLPSNRSSSSARPPASSFDPEATNIPSQRPNTS 513

Query: 510 TPSIPSSFTTRLPHLLLLVLYLV 526
           T S   S +  L      +L ++
Sbjct: 519 TTSAAYSLSISLSLFFFSILAIL 513

BLAST of Sed0027527 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 3.0e-24
Identity = 102/381 (26.77%), Postives = 167/381 (43.83%), Query Frame = 0

Query: 100 LHYTWIDIGTPSVSFLVALDAGSDLLWVPC-DCIQCAPLSASYYGSLDKDLNEYRPSRSS 159
           L++T I +G+P   + V +D GSD+LWV C  C +C P+       L   L+ Y    SS
Sbjct: 77  LYFTKIKLGSPPKEYYVQVDTGSDILWVNCAPCPKC-PVKT----DLGIPLSLYDSKTSS 136

Query: 160 SSKHISCSHNLCE---SGQSCQSPKQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSS 219
           +SK++ C  + C      ++C   K+ C Y + Y  + ++S G  I+D + L        
Sbjct: 137 TSKNVGCEDDFCSFIMQSETC-GAKKPCSYHVVY-GDGSTSDGDFIKDNITLEQVTGNLR 196

Query: 220 NCTIQAPVILGCGMKQSGGY-LSGVAPDGLFGLGLGEISVLSSLAKEGLVQNSFSLCF-N 279
              +   V+ GCG  QSG    +  A DG+ G G    S++S LA  G  +  FS C  N
Sbjct: 197 TAPLAQEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDN 256

Query: 280 EDGSGRIFFGDEGPASQQMTSFVSLDGKYEAYIVGVEACCIGNSCLKQTSFKA------- 339
            +G G    G+      + T  V     Y   + G++    G+      S  +       
Sbjct: 257 MNGGGIFAVGEVESPVVKTTPIVPNQVHYNVILKGMDV--DGDPIDLPPSLASTNGDGGT 316

Query: 340 LIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSVTFKGYPWKYCYKISSDAMPKVPSVTLL 399
           +IDSGT+  YLP+ +Y ++  +   K      +  + +    C+  +S+     P V L 
Sbjct: 317 IIDSGTTLAYLPQNLYNSLIEKITAKQQVKLHMVQETFA---CFSFTSNTDKAFPVVNLH 376

Query: 400 F--PLNNSFVVHDPVFPIYGDQGLAGFCF-----AILPADG-DIGILGQNYMTGYRMVFD 459
           F   L  S   HD +F +  D     +CF      +   DG D+ +LG   ++   +V+D
Sbjct: 377 FEDSLKLSVYPHDYLFSLREDM----YCFGWQSGGMTTQDGADVILLGDLVLSNKLVVYD 436

BLAST of Sed0027527 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 2.6e-23
Identity = 97/366 (26.50%), Postives = 164/366 (44.81%), Query Frame = 0

Query: 100 LHYTWIDIGTPSVSFLVALDAGSDLLWVPC-DCIQCAPLSASYYGSLDKDLNEYRPSRSS 159
           L++T I +G+P   + V +D GSD+LW+ C  C +C   +     +L+  L+ +  + SS
Sbjct: 73  LYFTKIKLGSPPKEYHVQVDTGSDILWINCKPCPKCPTKT-----NLNFRLSLFDMNASS 132

Query: 160 SSKHISCSHNLC---ESGQSCQSPKQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSS 219
           +SK + C  + C       SCQ P   C Y I Y  E+T S G  I+D+L L        
Sbjct: 133 TSKKVGCDDDFCSFISQSDSCQ-PALGCSYHIVYADEST-SDGKFIRDMLTLEQVTGDLK 192

Query: 220 NCTIQAPVILGCGMKQSGGYLSG-VAPDGLFGLGLGEISVLSSLAKEGLVQNSFSLCF-N 279
              +   V+ GCG  QSG   +G  A DG+ G G    SVLS LA  G  +  FS C  N
Sbjct: 193 TGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAATGDAKRVFSHCLDN 252

Query: 280 EDGSGRIFFGDEGPASQQMTSFVSLDGKYEAYIVGVE----ACCIGNSCLKQTSFKALID 339
             G G    G       + T  V     Y   ++G++    +  +  S ++      ++D
Sbjct: 253 VKGGGIFAVGVVDSPKVKTTPMVPNQMHYNVMLMGMDVDGTSLDLPRSIVRNGG--TIVD 312

Query: 340 SGTSFTYLPEEVYENIAMEFNRKLNTTSSVTFKGYPWKYCYKISSDAMPKVPSVTLLFPL 399
           SGT+  Y P+ +Y+++      +      +  + +    C+  S++     P V+  F  
Sbjct: 313 SGTTLAYFPKVLYDSLIETILARQPVKLHIVEETF---QCFSFSTNVDEAFPPVSFEFED 372

Query: 400 NNSFVV--HDPVFPIYGDQGLAGFCFAILPAD--GDIGILGQNYMTGYRMVFDRENLKLR 452
           +    V  HD +F +  +    G+    L  D   ++ +LG   ++   +V+D +N  + 
Sbjct: 373 SVKLTVYPHDYLFTLEEELYCFGWQAGGLTTDERSEVILLGDLVLSNKLVVYDLDNEVIG 426

BLAST of Sed0027527 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 6.3e-22
Identity = 106/364 (29.12%), Postives = 157/364 (43.13%), Query Frame = 0

Query: 84  PSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALDAGSDLLWVPCDCIQCAPLSASYYG 143
           P++   T+  GN     +   I IGTP     +  D GSDL W      QC P   S Y 
Sbjct: 120 PAKSGITLGSGN-----YIVTIGIGTPKHDLSLVFDTGSDLTWT-----QCEPCLGSCYS 179

Query: 144 SLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSPKQSCPYVIDYTTENTSSSGLLIQD 203
             +   N   PS SS+ +++SCS  +CE  +SC +   +C Y I Y  + + + G L ++
Sbjct: 180 QKEPKFN---PSSSSTYQNVSCSSPMCEDAESCSA--SNCVYSIVY-GDKSFTQGFLAKE 239

Query: 204 ILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGVAPDGLFGLGLGEISVLSSLAKEGL 263
              L       +N  +   V  GCG + + G   GVA  GL GLG G++S+ +       
Sbjct: 240 KFTL-------TNSDVLEDVYFGCG-ENNQGLFDGVA--GLLGLGPGKLSLPAQTTT--T 299

Query: 264 VQNSFSLC---FNEDGSGRIFFGDEG-PASQQMTSFVSLDGKYEAYIVGVEACCIGNSCL 323
             N FS C   F  + +G + FG  G   S + T   S    +  Y + +    +G+  L
Sbjct: 300 YNNIFSYCLPSFTSNSTGHLTFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKEL 359

Query: 324 KQT--SFK---ALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSVTFKGYPWKYCYKISS 383
             T  SF    A+IDSGT FT LP +VY  +   F  K+++  S +  G  +  CY  + 
Sbjct: 360 AITPNSFSTEGAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGL-FDTCYDFT- 419

Query: 384 DAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPADGDIGILGQNYMTGYR 439
             +  V   T+ F    S VV      I     ++  C A    D    I G    T   
Sbjct: 420 -GLDTVTYPTIAFSFAGSTVVELDGSGISLPIKISQVCLAFAGNDDLPAIFGNVQQTTLD 451

BLAST of Sed0027527 vs. ExPASy TrEMBL
Match: A0A6J1KSL9 (aspartic proteinase-like protein 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496886 PE=3 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 4.3e-263
Identity = 461/532 (86.65%), Postives = 496/532 (93.23%), Query Frame = 0

Query: 2   SLLLLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEY 61
           +L+L+L+MM  +S   A S I  +SR+ HRFS+EMKALRVS+S N+S+RVSWPEKGSMEY
Sbjct: 5   NLVLVLLMM--ISAHQALS-IAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSMEY 64

Query: 62  FQELLSRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALD 121
           +QEL+S D  R+ +KLGSRFQLLFPS+GS TIALGNDFGWLHY WIDIGTPSVSFLVALD
Sbjct: 65  YQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALD 124

Query: 122 AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSP 181
           AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPS SS+SKH+SCSHNLCESGQSCQSP
Sbjct: 125 AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSSSSTSKHLSCSHNLCESGQSCQSP 184

Query: 182 KQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGV 241
           KQSCPYVIDY TENTSSSGLLIQD+LHLSSGCE SSNCTIQAPV+LGCGMKQSGGYLSGV
Sbjct: 185 KQSCPYVIDYLTENTSSSGLLIQDVLHLSSGCENSSNCTIQAPVVLGCGMKQSGGYLSGV 244

Query: 242 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLD 301
           APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFV LD
Sbjct: 245 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVLLD 304

Query: 302 GKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSV 361
           GKYEAYIVGVEACCIGNSCL+QTSFKALIDSGTSFTYLPEEVYEN+ MEF+++LNTTS+V
Sbjct: 305 GKYEAYIVGVEACCIGNSCLEQTSFKALIDSGTSFTYLPEEVYENVVMEFDKRLNTTSTV 364

Query: 362 TFKGYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPAD 421
           TFKGYPWKYCYKIS+DAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILP D
Sbjct: 365 TFKGYPWKYCYKISADAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPTD 424

Query: 422 GDIGILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLAPAKETPPNPLPASEQQ 481
           GDIGILGQNYMTGYRMVFDRENLKL WSRA+C+DLSN+K+MPLAP+KETPPN LPA+EQQ
Sbjct: 425 GDIGILGQNYMTGYRMVFDRENLKLSWSRANCQDLSNEKKMPLAPSKETPPNSLPANEQQ 484

Query: 482 RVPGGRAVAPAVAGRAPAKPSAATPS-IPSSF-TTRLPHLLLLVLYLVSSSC 530
            V GG AVAPAVAGRAP+KPSAATP  IPS F T RL HLLLLVLYL S+ C
Sbjct: 485 SVSGGHAVAPAVAGRAPSKPSAATPCFIPSCFYTVRLLHLLLLVLYLGSTCC 533

BLAST of Sed0027527 vs. ExPASy TrEMBL
Match: A0A6J1HE55 (aspartic proteinase-like protein 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463362 PE=3 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 1.2e-262
Identity = 459/530 (86.60%), Postives = 495/530 (93.40%), Query Frame = 0

Query: 2   SLLLLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEY 61
           +L+L+L+MM  +S   A S I  +SR+ HRFS+EMKALRVS+S N+S+RVSWPEKGSMEY
Sbjct: 5   NLVLVLLMM--ISAHQAMS-IAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSMEY 64

Query: 62  FQELLSRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALD 121
           +QEL+S D  R+ +KLGSRFQLLFPS+GS TIALGNDFGWLHY WIDIGTPSVSFLVALD
Sbjct: 65  YQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALD 124

Query: 122 AGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSP 181
           AGSDLLW+PCDCIQCAPLSASYYGSLDKDLNEYRPS SS+SKHISCSHNLCESGQSCQSP
Sbjct: 125 AGSDLLWIPCDCIQCAPLSASYYGSLDKDLNEYRPSSSSTSKHISCSHNLCESGQSCQSP 184

Query: 182 KQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGV 241
           KQSCPYVIDY TENTSSSGLLIQD+LHLSSGCE SSNCTIQAPV+LGCGMKQSGGYLSGV
Sbjct: 185 KQSCPYVIDYLTENTSSSGLLIQDVLHLSSGCENSSNCTIQAPVVLGCGMKQSGGYLSGV 244

Query: 242 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLD 301
           APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFV LD
Sbjct: 245 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVLLD 304

Query: 302 GKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSV 361
           GKYEAYIVGVEACCIGNSCL+QTSFKALIDSGTSFTYLPEEVYEN+ MEF+++LNTTS+V
Sbjct: 305 GKYEAYIVGVEACCIGNSCLEQTSFKALIDSGTSFTYLPEEVYENVVMEFDKRLNTTSTV 364

Query: 362 TFKGYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPAD 421
           TFKGYPWKYCYKIS+DAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFA+LP D
Sbjct: 365 TFKGYPWKYCYKISADAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAVLPTD 424

Query: 422 GDIGILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLAPAKETPPNPLPASEQQ 481
           GDIGILGQNYMTGYRMVFDRENLKL WSRA+C+DLSN+K+MPLAP+KETPPNPLPA+EQQ
Sbjct: 425 GDIGILGQNYMTGYRMVFDRENLKLSWSRANCQDLSNEKKMPLAPSKETPPNPLPANEQQ 484

Query: 482 RVPGGRAVAPAVAGRAPAKPSAATPS-IPSSF-TTRLPHLLLLVLYLVSS 528
            V  G AVAPAVAGRAP+KPSAATP  IPS F T RL HLLLLV YLVS+
Sbjct: 485 SVSEGHAVAPAVAGRAPSKPSAATPCFIPSCFYTVRLLHLLLLVFYLVST 531

BLAST of Sed0027527 vs. ExPASy TrEMBL
Match: A0A6J1GFS3 (aspartic proteinase-like protein 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453783 PE=3 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.7e-259
Identity = 452/527 (85.77%), Postives = 489/527 (92.79%), Query Frame = 0

Query: 5   LLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEYFQE 64
           L+L+++  ++V  A S IT +SRL HRFS++MKALRVS S  + +R SWPEKGSMEY+QE
Sbjct: 6   LILLLLMVIAVHQAVS-ITFTSRLLHRFSEDMKALRVSGS-TTGVRASWPEKGSMEYYQE 65

Query: 65  LLSRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALDAGS 124
           L+S D  R+ +KLGSRFQ LFPS+GS TI LGNDFGWLHYTWIDIGTPSVSFLVALDAGS
Sbjct: 66  LVSGDFQRQKMKLGSRFQWLFPSEGSKTIELGNDFGWLHYTWIDIGTPSVSFLVALDAGS 125

Query: 125 DLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSPKQS 184
           DLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPS+SS+SKHISCSHNLC+SGQSCQSPKQS
Sbjct: 126 DLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSKSSTSKHISCSHNLCDSGQSCQSPKQS 185

Query: 185 CPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGVAPD 244
           CPYVIDYTTENTSSSGLLIQD+LHLSSGCE SSNCTIQAPVILGCGMKQSGGYLSGVAPD
Sbjct: 186 CPYVIDYTTENTSSSGLLIQDVLHLSSGCENSSNCTIQAPVILGCGMKQSGGYLSGVAPD 245

Query: 245 GLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLDGKY 304
           GLFGLGLGEISVLSSLAKEGLV NSFSLCFNEDGSGRIFFGDEGPASQQMTSFV LD KY
Sbjct: 246 GLFGLGLGEISVLSSLAKEGLVPNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVPLDEKY 305

Query: 305 EAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSVTFK 364
           EAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEE YENI MEF+++LN TS+V+FK
Sbjct: 306 EAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEAYENIVMEFDKRLNVTSTVSFK 365

Query: 365 GYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPADGDI 424
           GYPWKYCYKIS+DAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCF+ILPADGDI
Sbjct: 366 GYPWKYCYKISTDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFSILPADGDI 425

Query: 425 GILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLAPAKETPPNPLPASEQQRVP 484
           GILGQNYMTGYRMVFDR+NLKL WSRA+C+DLSN KEMP+APAKETPPNPLPA+EQQ  P
Sbjct: 426 GILGQNYMTGYRMVFDRDNLKLGWSRANCQDLSNDKEMPIAPAKETPPNPLPANEQQSAP 485

Query: 485 GGRAVAPAVAGRAPAKPSAATPSI-PSSF-TTRLPHLLLLVLYLVSS 528
           GG AVAPA+AGRAP+KPSAA P I PSSF + RLPHL+LLVL LVS+
Sbjct: 486 GGHAVAPAIAGRAPSKPSAAAPCIMPSSFYSIRLPHLILLVLCLVST 530

BLAST of Sed0027527 vs. ExPASy TrEMBL
Match: A0A6J1IU36 (aspartic proteinase-like protein 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478432 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 7.0e-258
Identity = 450/527 (85.39%), Postives = 486/527 (92.22%), Query Frame = 0

Query: 5   LLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEYFQE 64
           L+L+++  ++V    S IT +SRL HRFS +MKA RVS S  + +R SWPEKGSMEY+QE
Sbjct: 6   LILLLLMVIAVHQVVS-ITFTSRLLHRFSKDMKAFRVSGS-TTGVRASWPEKGSMEYYQE 65

Query: 65  LLSRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALDAGS 124
           L+S D  R+ +KLGSRFQ LFPS+GS TI LGNDFGWLHYTWIDIGTPSVSFLVALDAGS
Sbjct: 66  LVSGDFQRQKMKLGSRFQWLFPSEGSKTIELGNDFGWLHYTWIDIGTPSVSFLVALDAGS 125

Query: 125 DLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSPKQS 184
           DLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPS+SS+SKHISCSHNLC+SGQSCQSPKQS
Sbjct: 126 DLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSKSSTSKHISCSHNLCDSGQSCQSPKQS 185

Query: 185 CPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGVAPD 244
           CPYVIDYTTENTSSSGLLIQD+LHLSSGCE SSNCTIQAPVILGCGMKQSGGYLSGVAPD
Sbjct: 186 CPYVIDYTTENTSSSGLLIQDVLHLSSGCENSSNCTIQAPVILGCGMKQSGGYLSGVAPD 245

Query: 245 GLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLDGKY 304
           GLFGLGLGEISVLSSLAKEGLV NSFSLCFNEDGSGRIFFGDEGPASQQMTSFV LD KY
Sbjct: 246 GLFGLGLGEISVLSSLAKEGLVPNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVPLDEKY 305

Query: 305 EAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSVTFK 364
           EAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEE YENI MEF+++LN TS+V+FK
Sbjct: 306 EAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEAYENIVMEFDKRLNVTSTVSFK 365

Query: 365 GYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPADGDI 424
           GYPWKYCYKIS+DAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCF+ILPADGDI
Sbjct: 366 GYPWKYCYKISTDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFSILPADGDI 425

Query: 425 GILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLAPAKETPPNPLPASEQQRVP 484
           GILGQNYMTGYRMVFDR+NLKL WSRA+C+DLSN KEMP+APAKETPPNPLPA+EQQ  P
Sbjct: 426 GILGQNYMTGYRMVFDRDNLKLGWSRANCQDLSNDKEMPIAPAKETPPNPLPANEQQSAP 485

Query: 485 GGRAVAPAVAGRAPAKPSAATPS-IPSSF-TTRLPHLLLLVLYLVSS 528
           GG AVAPA+AGRAP+KPSAA P  IPSSF + RLPHL+LLVL LVS+
Sbjct: 486 GGHAVAPAIAGRAPSKPSAAAPCIIPSSFYSIRLPHLILLVLCLVST 530

BLAST of Sed0027527 vs. ExPASy TrEMBL
Match: A0A5D3CLH5 (Aspartic proteinase-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002590 PE=3 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 1.6e-257
Identity = 454/532 (85.34%), Postives = 488/532 (91.73%), Query Frame = 0

Query: 1   MSLLLLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSME 60
           MSL  L+M++  V       +IT +SR+ HRFS+EMKALRVS S N+S+RVSWPEKGSME
Sbjct: 1   MSLRNLVMLLLMVIFVHQAVSITFTSRILHRFSEEMKALRVSVSTNTSVRVSWPEKGSME 60

Query: 61  YFQELLSRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVAL 120
           Y+QEL+S D  R+ +KLGSRFQLLFPS+GS TIALGNDFGWLHYTWIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFRRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYTWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQS 180
           DAGSDLLW+PC+CIQCAPLSASYYGSLDKDLNEYRPS SS+SKHISCSHNLC+SGQSCQS
Sbjct: 121 DAGSDLLWIPCNCIQCAPLSASYYGSLDKDLNEYRPSSSSTSKHISCSHNLCDSGQSCQS 180

Query: 181 PKQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSG 240
           PKQSCPYVIDY TENTSSSGLLIQD+LHLSSGCE SSNCTIQAPVILGCGMKQSGGYLSG
Sbjct: 181 PKQSCPYVIDYITENTSSSGLLIQDVLHLSSGCENSSNCTIQAPVILGCGMKQSGGYLSG 240

Query: 241 VAPDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSL 300
           VAPDGLFGLGLGEISVLSSLAKE LVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFV L
Sbjct: 241 VAPDGLFGLGLGEISVLSSLAKEELVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVPL 300

Query: 301 DGKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSS 360
           DGKYE YIVGVEACCI NSCLKQTSFKALIDSGTSFTYLPEE YENI MEF+++LNTTS+
Sbjct: 301 DGKYETYIVGVEACCIENSCLKQTSFKALIDSGTSFTYLPEEAYENIVMEFDKRLNTTSA 360

Query: 361 VTFKGYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPA 420
           V+FKGYPWKYCYKIS+DAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPA
Sbjct: 361 VSFKGYPWKYCYKISADAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPA 420

Query: 421 DGDIGILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLAPAKETPPNPLPASEQ 480
           DGDIGILGQNYMTGYRMVFDR+NLKL WS A+C+DLSN+K+MPL PAKETPPNPLPA+EQ
Sbjct: 421 DGDIGILGQNYMTGYRMVFDRDNLKLGWSHANCQDLSNEKKMPLTPAKETPPNPLPANEQ 480

Query: 481 QRVPGGRAVAPAVAGRAPAKPS-AATPS-IPSSF-TTRLPHLLLLVLYLVSS 528
           Q   GG AVAPAVAGRAP+KPS AATP  IPS F + RLP+LLLL L LVSS
Sbjct: 481 QSASGGHAVAPAVAGRAPSKPSAAATPCFIPSKFYSIRLPYLLLLALCLVSS 532

BLAST of Sed0027527 vs. TAIR 10
Match: AT5G10080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 546.6 bits (1407), Expect = 2.2e-155
Identity = 293/529 (55.39%), Postives = 374/529 (70.70%), Query Frame = 0

Query: 9   MMFTVSVRPAESTIT--LSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEYFQELL 68
           ++F V     E T+    SSRL HRFSDE +A   + S++ SL    P K S+EY++ L 
Sbjct: 8   LLFCVLFLATEETLASLFSSRLIHRFSDEGRASIKTPSSSDSL----PNKQSLEYYRLLA 67

Query: 69  SRD--RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALDAGSDL 128
             D  R+ + LG++ Q L PS+GS TI+ GNDFGWLHYTWIDIGTPSVSFLVALD GS+L
Sbjct: 68  ESDFRRQRMNLGAKVQSLVPSEGSKTISSGNDFGWLHYTWIDIGTPSVSFLVALDTGSNL 127

Query: 129 LWVPCDCIQCAPLSASYYGSL-DKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSPKQSC 188
           LW+PC+C+QCAPL+++YY SL  KDLNEY PS SS+SK   CSH LC+S   C+SPK+ C
Sbjct: 128 LWIPCNCVQCAPLTSTYYSSLATKDLNEYNPSSSSTSKVFLCSHKLCDSASDCESPKEQC 187

Query: 189 PYVIDYTTENTSSSGLLIQDILHLSSGCEK---SSNCTIQAPVILGCGMKQSGGYLSGVA 248
           PY ++Y + NTSSSGLL++DILHL+        + + +++A V++GCG KQSG YL GVA
Sbjct: 188 PYTVNYLSGNTSSSGLLVEDILHLTYNTNNRLMNGSSSVKARVVIGCGKKQSGDYLDGVA 247

Query: 249 PDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLD- 308
           PDGL GLG  EISV S L+K GL++NSFSLCF+E+ SGRI+FGD GP+ QQ T F+ LD 
Sbjct: 248 PDGLMGLGPAEISVPSFLSKAGLMRNSFSLCFDEEDSGRIYFGDMGPSIQQSTPFLQLDN 307

Query: 309 GKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTSSV 368
            KY  YIVGVEACCIGNSCLKQTSF   IDSG SFTYLPEE+Y  +A+E +R +N TS  
Sbjct: 308 NKYSGYIVGVEACCIGNSCLKQTSFTTFIDSGQSFTYLPEEIYRKVALEIDRHINATSK- 367

Query: 369 TFKGYPWKYCYKISSDAMPKVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPAD 428
            F+G  W+YCY+  S A PKVP++ L F  NN+FV+H P+F     QGL  FC  I P+ 
Sbjct: 368 NFEGVSWEYCYE--SSAEPKVPAIKLKFSHNNTFVIHKPLFVFQQSQGLVQFCLPISPSG 427

Query: 429 GD-IGILGQNYMTGYRMVFDRENLKLRWSRASCEDLSNQKEMPLA-PAKETPPNPLPASE 488
            + IG +GQNYM GYRMVFDREN+KL WS + C++  ++ E P A P   + PNPLP  E
Sbjct: 428 QEGIGSIGQNYMRGYRMVFDRENMKLGWSPSKCQE--DKIEPPQASPGSTSSPNPLPTDE 487

Query: 489 QQRVPGGRAVAPAVAGRAPAKPSAATPSIPSSFTTRLPHLLLLVLYLVS 527
           QQ   GG AV+PA+AG+ P+K  +++ S   S   RL + LLL+ +L S
Sbjct: 488 QQS-RGGHAVSPAIAGKTPSKTPSSSSSYSFSSIMRLFNSLLLLHWLAS 526

BLAST of Sed0027527 vs. TAIR 10
Match: AT4G35880.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 310.1 bits (793), Expect = 3.5e-84
Identity = 177/465 (38.06%), Postives = 272/465 (58.49%), Query Frame = 0

Query: 1   MSLLLLLMMMFTVSVRPAESTITLSSRLF-----HRFSDEMKALRVSKSANSSLRVSWPE 60
           MS       +F + +    S  + + R+F     HRFSDE+K      S ++     +P 
Sbjct: 1   MSCCFFKTTLFLIPILMLLSFGSCNGRIFTFEMHHRFSDEVK----QWSDSTGRFAKFPP 60

Query: 61  KGSMEYFQELLSRD-----RKNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTP 120
           KGS EYF  L+ RD     R+  +  S  +        N+ +  +  G+LHYT + +GTP
Sbjct: 61  KGSFEYFNALVLRDWLIRGRRLSESESESESSLTFSDGNSTSRISSLGFLHYTTVKLGTP 120

Query: 121 SVSFLVALDAGSDLLWVPCDCIQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLC 180
            + F+VALD GSDL WVPCDC +CAP   + Y S + +L+ Y P  S+++K ++C+++LC
Sbjct: 121 GMRFMVALDTGSDLFWVPCDCGKCAPTEGATYAS-EFELSIYNPKVSTTNKKVTCNNSLC 180

Query: 181 ESGQSCQSPKQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMK 240
                C     +CPY++ Y +  TS+SG+L++D++HL++  E  +   ++A V  GCG  
Sbjct: 181 AQRNQCLGTFSTCPYMVSYVSAQTSTSGILMEDVMHLTT--EDKNPERVEAYVTFGCGQV 240

Query: 241 QSGGYLSGVAPDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNEDGSGRIFFGDEGPASQ 300
           QSG +L   AP+GLFGLG+ +ISV S LA+EGLV +SFS+CF  DG GRI FGD+G + Q
Sbjct: 241 QSGSFLDIAAPNGLFGLGMEKISVPSVLAREGLVADSFSMCFGHDGVGRISFGDKGSSDQ 300

Query: 301 QMTSFVSLDGKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFN 360
           + T F +L+  +  Y + V    +G + L    F AL D+GTSFTYL + +Y  ++  F+
Sbjct: 301 EETPF-NLNPSHPNYNITVTRVRVGTT-LIDDEFTALFDTGTSFTYLVDPMYTTVSESFH 360

Query: 361 RKLNTTSSVTFKGYPWKYCYKISSDAMPK-VPSVTLLFPLNNSFVVHDPVFPIYGDQGLA 420
            +            P++YCY +S+DA    +PS++L    N+ F ++DP+  +   +G  
Sbjct: 361 SQAQDKRHSPDSRIPFEYCYDMSNDANASLIPSLSLTMKGNSHFTINDPII-VISTEGEL 420

Query: 421 GFCFAILPADGDIGILGQNYMTGYRMVFDRENLKLRWSRASCEDL 455
            +C AI+ +  ++ I+GQNYMTGYR+VFDRE L L W +  C D+
Sbjct: 421 VYCLAIVKS-SELNIIGQNYMTGYRVVFDREKLVLAWKKFDCYDI 454

BLAST of Sed0027527 vs. TAIR 10
Match: AT2G17760.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 278.9 bits (712), Expect = 8.6e-75
Identity = 185/503 (36.78%), Postives = 262/503 (52.09%), Query Frame = 0

Query: 30  HRFSDEMKALRVSKSANSSLRVSWPEKGSMEYFQELLSRDR--KNLKLGSRFQLLFPSQG 89
           HRFSD++  +              P + S +Y++ +  RDR  +  +L +  Q L     
Sbjct: 39  HRFSDQVVGVLPGDGL--------PNRDSSKYYRVMAHRDRLIRGRRLANEDQSLVTFSD 98

Query: 90  SNTIALGNDFGWLHYTWIDIGTPSVSFLVALDAGSDLLWVPCDCIQCA-PLSASYYGSLD 149
            N     +  G+LHY  + +GTPS  F+VALD GSDL W+PCDC  C   L A   G   
Sbjct: 99  GNETVRVDALGFLHYANVTVGTPSDWFMVALDTGSDLFWLPCDCTNCVRELKAP--GGSS 158

Query: 150 KDLNEYRPSRSSSSKHISCSHNLCESGQSCQSPKQSCPYVIDYTTENTSSSGLLIQDILH 209
            DLN Y P+ SS+S  + C+  LC  G  C SP+  CPY I Y +  TSS+G+L++D+LH
Sbjct: 159 LDLNIYSPNASSTSTKVPCNSTLCTRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLH 218

Query: 210 LSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGVAPDGLFGLGLGEISVLSSLAKEGLVQN 269
           L S  +KSS   I A V  GCG  Q+G +  G AP+GLFGLGL +ISV S LAKEG+  N
Sbjct: 219 LVSN-DKSSK-AIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAAN 278

Query: 270 SFSLCFNEDGSGRIFFGDEGPASQQMTSFVSLDGKYEAYIVGVEACCIGNSCLKQTSFKA 329
           SFS+CF  DG+GRI FGD+G   Q+ T  +++   +  Y + V    +G +      F A
Sbjct: 279 SFSMCFGNDGAGRISFGDKGSVDQRETP-LNIRQPHPTYNITVTKISVGGN-TGDLEFDA 338

Query: 330 LIDSGTSFTYLPEEVYENIAMEFNR-KLNTTSSVTFKGYPWKYCYKISSDAMP-KVPSVT 389
           + DSGTSFTYL +  Y  I+  FN   L+     T    P++YCY +S +    + P+V 
Sbjct: 339 VFDSGTSFTYLTDAAYTLISESFNSLALDKRYQTTDSELPFEYCYALSPNKDSFQYPAVN 398

Query: 390 LLFPLNNSFVVHDPVFPIYGDQGLAGFCFAILPADGDIGILGQNYMTGYRMVFDRENLKL 449
           L     +S+ V+ P+  +   +    +C AI+  + DI I+GQN+MTGYR+VFDRE L L
Sbjct: 399 LTMKGGSSYPVYHPLV-VIPMKDTDVYCLAIMKIE-DISIIGQNFMTGYRVVFDREKLIL 458

Query: 450 RWSRASCEDLSNQKEMPLAPAKETPPNPLPA--SEQQRVPGGRAVAPAVAGRAPAKPSAA 509
            W  + C               ET    LP+  S     P   +  P        +P+ +
Sbjct: 459 GWKESDCY------------TGETSARTLPSNRSSSSARPPASSFDPEATNIPSQRPNTS 513

Query: 510 TPSIPSSFTTRLPHLLLLVLYLV 526
           T S   S +  L      +L ++
Sbjct: 519 TTSAAYSLSISLSLFFFSILAIL 513

BLAST of Sed0027527 vs. TAIR 10
Match: AT3G51330.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 255.8 bits (652), Expect = 7.8e-68
Identity = 173/486 (35.60%), Postives = 252/486 (51.85%), Query Frame = 0

Query: 4   LLLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEYFQ 63
           +LL +++    +   E++   S  + H FSD +K        +  L    PEKGS+EYF+
Sbjct: 9   VLLSLLVVCWGLERCEASGKFSFEVHHMFSDRVK-------QSLGLDDLVPEKGSLEYFK 68

Query: 64  ELLSRDR----KNLKLGSRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALD 123
            L  RDR    + L   +    +   +G+ TI++ +  G+LHY  + +GTP+  FLVALD
Sbjct: 69  VLAQRDRLIRGRGLASNNEETPITFMRGNRTISI-DLLGFLHYANVSVGTPATWFLVALD 128

Query: 124 AGSDLLWVPCDCIQCAPLSASYYG-SLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQS 183
            GSDL W+PC+C           G S  + LN Y P+ SS+S  I CS + C     C S
Sbjct: 129 TGSDLFWLPCNCGSTCIRDLKEVGLSQSRPLNLYSPNTSSTSSSIRCSDDRCFGSSRCSS 188

Query: 184 PKQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSG 243
           P  SCPY I Y +++T ++G L +D+LHL +  E      ++A + LGCG  Q+G   S 
Sbjct: 189 PASSCPYQIQYLSKDTFTTGTLFEDVLHLVT--EDEGLEPVKANITLGCGKNQTGFLQSS 248

Query: 244 VAPDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNE--DGSGRIFFGDEGPASQQMTSFV 303
            A +GL GLGL + SV S LAK  +  NSFS+CF    D  GRI FGD+G   Q  T  +
Sbjct: 249 AAVNGLLGLGLKDYSVPSILAKAKITANSFSMCFGNIIDVVGRISFGDKGYTDQMETPLL 308

Query: 304 SLDGKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTT 363
             +     Y V V    +G   +      AL D+GTSFT+L E  Y  I   F+  +   
Sbjct: 309 PTEPS-PTYAVSVTEVSVGGDAV-GVQLLALFDTGTSFTHLLEPEYGLITKAFDDHVTDK 368

Query: 364 SSVTFKGYPWKYCYKISSDAMPKV-PSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAI 423
                   P+++CY +S +    + P V + F   +   + +P+F ++ +   A +C  I
Sbjct: 369 RRPIDPELPFEFCYDLSPNKTTILFPRVAMTFEGGSQMFLRNPLFIVWNEDNSAMYCLGI 428

Query: 424 LPA-DGDIGILGQNYMTGYRMVFDRENLKLRWSRASC-EDLSNQKEMPLAPAKETP---- 476
           L + D  I I+GQN+M+GYR+VFDRE + L W R+ C ED S +   P  P  E P    
Sbjct: 429 LKSVDFKINIIGQNFMSGYRIVFDRERMILGWKRSDCFEDESLESTTPPPPETEAPSPSA 482

BLAST of Sed0027527 vs. TAIR 10
Match: AT3G51350.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 229.2 bits (583), Expect = 7.9e-60
Identity = 165/502 (32.87%), Postives = 246/502 (49.00%), Query Frame = 0

Query: 4   LLLLMMMFTVSVRPAESTITLSSRLFHRFSDEMKALRVSKSANSSLRVSWPEKGSMEYFQ 63
           +LL +++        E+T      + H FSD +K        +  L    PE+GS+EYF+
Sbjct: 9   VLLSVLVVCWGFERCEATGKFGFEVHHIFSDSVK-------QSLGLGDLVPEQGSLEYFK 68

Query: 64  ELLSRDRKNLKLG---SRFQLLFPSQGSNTIALGNDFGWLHYTWIDIGTPSVSFLVALDA 123
            L  RDR     G   +  +      G N        G L+Y  + +GTP  SFLVALD 
Sbjct: 69  VLAHRDRLIRGRGLASNNDETPITFDGGNLTVSVKLLGSLYYANVSVGTPPSSFLVALDT 128

Query: 124 GSDLLWVPCDC-IQCAPLSASYYGSLDKDLNEYRPSRSSSSKHISCSHNLCESGQSCQSP 183
           GSDL W+PC+C   C              LN Y P+ S++S  I CS   C   + C SP
Sbjct: 129 GSDLFWLPCNCGTTCIRDLEDIGVPQSVPLNLYTPNASTTSSSIRCSDKRCFGSKKCSSP 188

Query: 184 KQSCPYVIDYTTENTSSSGLLIQDILHLSSGCEKSSNCTIQAPVILGCGMKQSGGYLSGV 243
              CPY I Y + +T + G L+QD+LHL++  E  +   ++A V LGCG KQ+G +    
Sbjct: 189 SSICPYQISY-SNSTGTKGTLLQDVLHLAT--EDENLTPVKANVTLGCGQKQTGLFQRNN 248

Query: 244 APDGLFGLGLGEISVLSSLAKEGLVQNSFSLCFNE--DGSGRIFFGDEGPASQQMTSFVS 303
           + +G+ GLG+   SV S LAK  +  NSFS+CF       GRI FGD G   Q+ T F+S
Sbjct: 249 SVNGVLGLGIKGYSVPSLLAKANITANSFSMCFGRVIGNVGRISFGDRGYTDQEETPFIS 308

Query: 304 LDGKYEAYIVGVEACCIGNSCLKQTSFKALIDSGTSFTYLPEEVYENIAMEFNRKLNTTS 363
           +     AY V +    +    +    F A  D+G+SFT+L E  Y  +   F+  +    
Sbjct: 309 V-APSTAYGVNISGVSVAGDPVDIRLF-AKFDTGSSFTHLREPAYGVLTKSFDELVEDRR 368

Query: 364 SVTFKGYPWKYCYKISSDAMP-KVPSVTLLFPLNNSFVVHDPVFPIYGDQGLAGFCFAIL 423
                  P+++CY +S +A   + P V + F   +  ++++P F     +G   +C  +L
Sbjct: 369 RPVDPELPFEFCYDLSPNATTIQFPLVEMTFIGGSKIILNNPFFTARTQEGNVMYCLGVL 428

Query: 424 PADG-DIGILGQNYMTGYRMVFDRENLKLRWSRASC-EDLSNQKEMPLAPAKETPPNPLP 483
            + G  I ++GQN++ GYR+VFDRE + L W ++ C ED S +   P  P  E P   + 
Sbjct: 429 KSVGLKINVIGQNFVAGYRIVFDRERMILGWKQSLCFEDESLESTTPPPPEVEAPAPSVS 488

Query: 484 ASEQQRVPGGRAVAPAVAGRAP 497
           A      P  R++ P V+   P
Sbjct: 489 A------PPPRSLPPTVSATPP 492