Sed0026391 (gene) Chayote v1

BLAST of Sed0026391 vs. NCBI nr
Match: XP_038887593.1 (mediator of RNA polymerase II transcription subunit 33B isoform X1 [Benincasa hispida])

HSP 1 Score: 2332.0 bits (6042), Expect = 0.0e+00
Identity = 1195/1336 (89.45%), Postives = 1262/1336 (94.46%), Query Frame = 0

Query: 23   MAVSDPPPGQLPVVSGLWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAH 82
            MAVS  PPGQL  +SGLWD VLELTKSAQ KN DPLLWA+QLSSTL+SA VSLPS+ELA 
Sbjct: 1    MAVSTQPPGQLQEISGLWDSVLELTKSAQDKNCDPLLWAVQLSSTLNSAGVSLPSVELAQ 60

Query: 83   VLVSHICWDNHVPITWKFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLN 142
            +LVSHICWDNHVPI WKFL++AMTA IVPPLLVIALLSTRAIPYRKLR AAYRLYLE+L+
Sbjct: 61   LLVSHICWDNHVPIMWKFLEKAMTARIVPPLLVIALLSTRAIPYRKLRPAAYRLYLEVLS 120

Query: 143  RHIFSLTLQINGPNYQRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLD 202
            RHIFSLT QINGPNYQRIMQTIDDVLHL+QIFG+QTCEPG+LMVELFFSIVWQLLDASLD
Sbjct: 121  RHIFSLTSQINGPNYQRIMQTIDDVLHLTQIFGVQTCEPGVLMVELFFSIVWQLLDASLD 180

Query: 203  DEELLVLPAEERSAWLIRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQ 262
            DE LL LP EE+S WLIRPQ HDM+LDVH+S  EKRTEN E LLK+N  KAIE+IGQFLQ
Sbjct: 181  DEGLLALPGEEKSVWLIRPQLHDMELDVHDSFGEKRTENSESLLKVNTAKAIEIIGQFLQ 240

Query: 263  NKKTARILCLAQQNMPLHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSR 322
            NKKTARILCLA +NMPLHWAGFA+RL+LLAANSVVL+NTKLIT EVLLHWTSDK +LLS+
Sbjct: 241  NKKTARILCLALRNMPLHWAGFAQRLKLLAANSVVLRNTKLITPEVLLHWTSDKSKLLSQ 300

Query: 323  EGETTSQLVFRDVIMASGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVER 382
            EG+ T QL FRDV MASGSLFSSAGQSHGVN SALWLPIDLFLEDAMDGSQVLATSAVER
Sbjct: 301  EGK-TCQLEFRDV-MASGSLFSSAGQSHGVNWSALWLPIDLFLEDAMDGSQVLATSAVER 360

Query: 383  LICLTKSLRAVNDTSWHHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAV 442
            LICL KSLRAVND+SWH+TFLGLWIAALRLIQRER+PSEGPVPRLDTCLCMLLSI+TLAV
Sbjct: 361  LICLIKSLRAVNDSSWHNTFLGLWIAALRLIQRERDPSEGPVPRLDTCLCMLLSISTLAV 420

Query: 443  TIIIEEEECELKEEECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVAN 502
            TIIIEEEE E+KE+ECS SKSRDE+QSSG CRKGLITSLQMLGEYESLL PPQSVIAVAN
Sbjct: 421  TIIIEEEEGEVKEDECSASKSRDEKQSSGMCRKGLITSLQMLGEYESLLIPPQSVIAVAN 480

Query: 503  QAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPGY 562
            QAAAKAVMFI+GVAVGNEY+DCVSM+DTPI CSGNMRHLIVEACISRNLLDTSAYFWPGY
Sbjct: 481  QAAAKAVMFISGVAVGNEYHDCVSMSDTPINCSGNMRHLIVEACISRNLLDTSAYFWPGY 540

Query: 563  VNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSVD 622
             NAR SQVP S S QVVGWSSFMKGSSLTPSM+NALVATPASSLAEIEKIYEIA NGS D
Sbjct: 541  ANARSSQVPRSASSQVVGWSSFMKGSSLTPSMVNALVATPASSLAEIEKIYEIAINGSGD 600

Query: 623  EKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLVG 682
            EKISAAS+LCGASLVRGWN+QEH  LFI+RLLSPPIP DYSG++SYLIDYA FLN LLVG
Sbjct: 601  EKISAASILCGASLVRGWNLQEHTALFISRLLSPPIPTDYSGSESYLIDYAPFLNVLLVG 660

Query: 683  ISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTCRAVFSLAFTLLL 742
            ISSVDCVQIFSLHGMVPLLAGQLMPICEAFGS  PKSWILTSGEELTC AVFSLAFTLLL
Sbjct: 661  ISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSCSPKSWILTSGEELTCHAVFSLAFTLLL 720

Query: 743  RLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLKFS 802
            RLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRN+QLASFG+SPKDRL+A+RLSKLLKFS
Sbjct: 721  RLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNSQLASFGKSPKDRLKARRLSKLLKFS 780

Query: 803  VKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLAS 862
            ++P++MD+FPKLKGWYRQHQECIASIL GL+PGAPVHQIVDAL+ MMF+KINR GQSL S
Sbjct: 781  LEPIFMDSFPKLKGWYRQHQECIASILSGLIPGAPVHQIVDALLTMMFRKINRAGQSLTS 840

Query: 863  TTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDLA 922
            TTSGSSN+SGSA+EEASIKLKVPAWDIL+ATPFVLDAALTACAHGRLS RDLATGLKDLA
Sbjct: 841  TTSGSSNSSGSANEEASIKLKVPAWDILEATPFVLDAALTACAHGRLSPRDLATGLKDLA 900

Query: 923  DFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVPS 982
            DFLPA+FATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIK ILAATGVDVPS
Sbjct: 901  DFLPASFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKKILAATGVDVPS 960

Query: 983  LAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIASL 1042
            LAVGG+SPA LPLPLAALISLTITYKLDKASERLLALVGPALNS+AASCSWPCTPIIASL
Sbjct: 961  LAVGGSSPAMLPLPLAALISLTITYKLDKASERLLALVGPALNSLAASCSWPCTPIIASL 1020

Query: 1043 WSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGHG 1102
            W+QKVKRWNDFLVFSASRTVFHH  DAVVQLLKSCFTSTLGLGNSNV SSGGVG+LLGHG
Sbjct: 1021 WAQKVKRWNDFLVFSASRTVFHHTSDAVVQLLKSCFTSTLGLGNSNVNSSGGVGTLLGHG 1080

Query: 1103 FGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKLK 1162
            FGS+VLGGMSPVAPGILYLRVHRS+RD LF+VEEIVS+LMLSVRDIAVSGLPKEKAEKLK
Sbjct: 1081 FGSHVLGGMSPVAPGILYLRVHRSVRDVLFLVEEIVSLLMLSVRDIAVSGLPKEKAEKLK 1140

Query: 1163 KAKYGMRYEQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVER 1222
            K KYGMRYEQVSFASAMARVKLAASLGASLVWI GGSGLVQSLFKETLPSWFLSV SVER
Sbjct: 1141 KTKYGMRYEQVSFASAMARVKLAASLGASLVWISGGSGLVQSLFKETLPSWFLSVQSVER 1200

Query: 1223 EGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLASALDGKF 1282
            EGVE GGMVAVL GYALAFFSVLCGTFSWGIDS+SSASKRRAKILDS LEFLASALDGKF
Sbjct: 1201 EGVEYGGMVAVLRGYALAFFSVLCGTFSWGIDSSSSASKRRAKILDSFLEFLASALDGKF 1260

Query: 1283 SIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESGG 1342
            SIGCDW TWRAYVSGFVSLMVRCAPRWLLEVD+NLLKRLS GLRQLNEE+LALALL SGG
Sbjct: 1261 SIGCDWATWRAYVSGFVSLMVRCAPRWLLEVDLNLLKRLSNGLRQLNEEQLALALLGSGG 1320

Query: 1343 VNAMGAAAELIIEGGF 1359
            V AMGAAAELIIEGGF
Sbjct: 1321 VTAMGAAAELIIEGGF 1334

BLAST of Sed0026391 vs. NCBI nr
Match: XP_022155567.1 (mediator of RNA polymerase II transcription subunit 33B-like [Momordica charantia])

HSP 1 Score: 2330.1 bits (6037), Expect = 0.0e+00
Identity = 1190/1337 (89.01%), Postives = 1261/1337 (94.32%), Query Frame = 0

Query: 23   MAVSDPPPGQLPVVSGLWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAH 82
            M VS  PP QL  ++GLWD VLELTKSAQ KN DPLLWA+QLSS+L+SA VSLPS+ELA 
Sbjct: 1    MVVSVQPPSQLQGMAGLWDSVLELTKSAQDKNCDPLLWAVQLSSSLNSAGVSLPSIELAQ 60

Query: 83   VLVSHICWDNHVPITWKFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLN 142
            +LVSHICWDNHVPI WKFL++AMTA IVPPLLV+ALLSTRAIPYRKLR AAYRLYLELL+
Sbjct: 61   LLVSHICWDNHVPIMWKFLEKAMTAKIVPPLLVVALLSTRAIPYRKLRPAAYRLYLELLS 120

Query: 143  RHIFSLTLQINGPNYQRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLD 202
            RH+FS T QINGPNYQRIMQTIDDVLHLSQIF LQ CEPGLLMVELFFSIVWQLLDASLD
Sbjct: 121  RHVFSSTSQINGPNYQRIMQTIDDVLHLSQIFSLQACEPGLLMVELFFSIVWQLLDASLD 180

Query: 203  DEELLVLPAEERSAWLIRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQ 262
            DE LLVLPAEERSAWLIRPQPHDM+LDVH+S SEKRTEN E LLK+N  KAIE+IGQFLQ
Sbjct: 181  DEGLLVLPAEERSAWLIRPQPHDMELDVHDSFSEKRTENSESLLKVNTAKAIEIIGQFLQ 240

Query: 263  NKKTARILCLAQQNMPLHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSR 322
            NKKTARIL LA +NMPLHWAGFA+RLQLLAANS VL+NTKLIT EVLLHWTSDKHRLLSR
Sbjct: 241  NKKTARILYLAHRNMPLHWAGFAQRLQLLAANSAVLRNTKLITPEVLLHWTSDKHRLLSR 300

Query: 323  EGETTSQLVFRDVIMASGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVER 382
            EG+ TSQ  FR+V MASGSLFSSAGQSHGVN S LWLPIDLFLEDAMDGSQVLATSAVER
Sbjct: 301  EGK-TSQQEFRNV-MASGSLFSSAGQSHGVNWSTLWLPIDLFLEDAMDGSQVLATSAVER 360

Query: 383  LICLTKSLRAVNDTSWHHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAV 442
            LICL KSL+AVNDTSWH+TF+GLWIAALRLIQRER+PSEGPVPRLDTCLCMLLSITTLAV
Sbjct: 361  LICLIKSLQAVNDTSWHNTFMGLWIAALRLIQRERDPSEGPVPRLDTCLCMLLSITTLAV 420

Query: 443  TIIIEEEECELKEE-ECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVA 502
            TIIIEE+E ELKEE ECSPSK RDE++ SGKCRKGLITSLQMLGEYE LL PPQSV A+A
Sbjct: 421  TIIIEEDEGELKEEDECSPSKGRDEKKCSGKCRKGLITSLQMLGEYEGLLTPPQSVTAIA 480

Query: 503  NQAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPG 562
            NQAAAKAVMFI+GVAVGNEYYDCVSMNDTP+ CSGNMRHLIVEACISRNLLDTS YFWPG
Sbjct: 481  NQAAAKAVMFISGVAVGNEYYDCVSMNDTPVNCSGNMRHLIVEACISRNLLDTSVYFWPG 540

Query: 563  YVNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSV 622
            YVNAR SQVP S SGQVVGWSSFMKGSSLT SM++ALVATPASSLAEIEKIYEIA NGS 
Sbjct: 541  YVNARSSQVPRSASGQVVGWSSFMKGSSLTLSMVDALVATPASSLAEIEKIYEIAVNGSG 600

Query: 623  DEKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLV 682
            DEKISAAS+LCG SLVRGWN+QEH VLFIARLLSPPIPADYSG+DSYLIDYA FLN LLV
Sbjct: 601  DEKISAASILCGESLVRGWNLQEHTVLFIARLLSPPIPADYSGSDSYLIDYAPFLNVLLV 660

Query: 683  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTCRAVFSLAFTLL 742
            GISSVDCVQIFSLHGMVPLLAGQLMPICEAFG SPPKSW+LTSGEELTC AVFSLAFTLL
Sbjct: 661  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGLSPPKSWVLTSGEELTCHAVFSLAFTLL 720

Query: 743  LRLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLKF 802
            LRLWRFHHPPVENVK DARPVGSQLTPEYLLLVRN+QLASFG+SPKDR + +RLSKLLKF
Sbjct: 721  LRLWRFHHPPVENVKRDARPVGSQLTPEYLLLVRNSQLASFGKSPKDRFKVRRLSKLLKF 780

Query: 803  SVKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLA 862
            S++P++MD+FPKLKGWYRQHQECIASIL GLVPGAPVHQIVDAL+ MMF+KINRGG SL 
Sbjct: 781  SLEPIFMDSFPKLKGWYRQHQECIASILSGLVPGAPVHQIVDALLTMMFRKINRGGHSLT 840

Query: 863  STTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDL 922
            STTSGSSN+SGSA+EEASIKLKVPAWDIL+ATPFVLDAALTACAHGRLS RDLATGLKDL
Sbjct: 841  STTSGSSNSSGSANEEASIKLKVPAWDILEATPFVLDAALTACAHGRLSPRDLATGLKDL 900

Query: 923  ADFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVP 982
            ADFLPA+FATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIK ILAATGVDVP
Sbjct: 901  ADFLPASFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKKILAATGVDVP 960

Query: 983  SLAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIAS 1042
            SLAVGG SPA LPLPLAALISLTITYKLDKASERLLALVGPALN++AASCSWPCTPIIAS
Sbjct: 961  SLAVGGNSPAMLPLPLAALISLTITYKLDKASERLLALVGPALNTLAASCSWPCTPIIAS 1020

Query: 1043 LWSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGH 1102
            LW+QKVKRWNDFLVFSASRTVFHHN DAVVQLLKSCFTSTLGLGNSN+ S+GGVG+LLGH
Sbjct: 1021 LWAQKVKRWNDFLVFSASRTVFHHNSDAVVQLLKSCFTSTLGLGNSNLNSNGGVGTLLGH 1080

Query: 1103 GFGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKL 1162
            GFGS+VLGGMSPVAPGILYLRVHRS+RD+LFMVEEIVS+LMLSVRDIAVSGLP+EKAEKL
Sbjct: 1081 GFGSHVLGGMSPVAPGILYLRVHRSVRDALFMVEEIVSLLMLSVRDIAVSGLPREKAEKL 1140

Query: 1163 KKAKYGMRYEQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVE 1222
            KK K+GMRYEQVSFASAM+RVKLAASLGASLVWI GGSGLVQSLFKETLPSWFLSVHS+E
Sbjct: 1141 KKTKHGMRYEQVSFASAMSRVKLAASLGASLVWISGGSGLVQSLFKETLPSWFLSVHSLE 1200

Query: 1223 REGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLASALDGK 1282
            REGVE GGMVAVLGGYALAFFSVLCGTFSWGIDS SSASKRRAKILDSHLEFLASALDGK
Sbjct: 1201 REGVEYGGMVAVLGGYALAFFSVLCGTFSWGIDSVSSASKRRAKILDSHLEFLASALDGK 1260

Query: 1283 FSIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESG 1342
            FSIGCDW TWRAYVSGFVSLMVRCAP+W++EVDVN+LKRLS GLRQL+EEELALALLESG
Sbjct: 1261 FSIGCDWATWRAYVSGFVSLMVRCAPKWVVEVDVNILKRLSNGLRQLSEEELALALLESG 1320

Query: 1343 GVNAMGAAAELIIEGGF 1359
            GV AMGAAAELIIEGGF
Sbjct: 1321 GVTAMGAAAELIIEGGF 1335

BLAST of Sed0026391 vs. NCBI nr
Match: XP_023554812.1 (mediator of RNA polymerase II transcription subunit 33B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2328.1 bits (6032), Expect = 0.0e+00
Identity = 1185/1337 (88.63%), Postives = 1257/1337 (94.02%), Query Frame = 0

Query: 23   MAVSDPPPGQLPVVSGLWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAH 82
            MAVS  PPGQL  ++G+WD VLELTKSAQ KN DPLLWA+QLSS+L+SA VSLPS+ELAH
Sbjct: 1    MAVSAQPPGQLQGIAGVWDTVLELTKSAQEKNSDPLLWAVQLSSSLNSASVSLPSVELAH 60

Query: 83   VLVSHICWDNHVPITWKFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLN 142
            +LVSHICWDNHVPI WKFL++AMTA IVPPLLVIALLSTRAIPYRKLR AAYRLYLELL+
Sbjct: 61   LLVSHICWDNHVPIMWKFLEKAMTARIVPPLLVIALLSTRAIPYRKLRPAAYRLYLELLS 120

Query: 143  RHIFSLTLQINGPNYQRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLD 202
            RH+FS TL++NGPNY RIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVW LLDASLD
Sbjct: 121  RHVFSSTLEVNGPNYPRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWHLLDASLD 180

Query: 203  DEELLVLPAEERSAWLIRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQ 262
            DE LL LPAEERS WLIRPQPHDM+LDVH+S  EK+TEN E LLK+N  KAIE+IGQFLQ
Sbjct: 181  DEGLLELPAEERSVWLIRPQPHDMELDVHDSFGEKKTENSENLLKVNTAKAIEIIGQFLQ 240

Query: 263  NKKTARILCLAQQNMPLHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSR 322
            NKKTARILCLA QNMPLHWAGFA+RLQLLAANSVVL+NTKLIT EVLLHWTSDKHR LS+
Sbjct: 241  NKKTARILCLAHQNMPLHWAGFAQRLQLLAANSVVLRNTKLITPEVLLHWTSDKHRFLSQ 300

Query: 323  EGETTSQLVFRDVIMASGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVER 382
            EG+TTSQL F DV MASGSLFSSAGQSHGVN SALWLPIDLFLEDAMDGSQVLATSAVER
Sbjct: 301  EGKTTSQLEFHDV-MASGSLFSSAGQSHGVNWSALWLPIDLFLEDAMDGSQVLATSAVER 360

Query: 383  LICLTKSLRAVNDTSWHHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAV 442
            LICL KSLRAVND SWH+TFLGLWIAALRLIQRER+PSEGPVPRLDTCLCMLLSITTLAV
Sbjct: 361  LICLIKSLRAVNDASWHNTFLGLWIAALRLIQRERDPSEGPVPRLDTCLCMLLSITTLAV 420

Query: 443  TIIIEEEECELKEE-ECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVA 502
            TIIIEEEE ELKEE ECSPSKSRDE+QSSGK R+GLITSLQMLGEYESLL PPQSVI VA
Sbjct: 421  TIIIEEEEGELKEEDECSPSKSRDEKQSSGKRRQGLITSLQMLGEYESLLTPPQSVIVVA 480

Query: 503  NQAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPG 562
            NQAAAKAVMFI+GVAVGNEYYDCVSMNDTPI CSGNMRHLIVEACISRNLLDTSAYFWPG
Sbjct: 481  NQAAAKAVMFISGVAVGNEYYDCVSMNDTPINCSGNMRHLIVEACISRNLLDTSAYFWPG 540

Query: 563  YVNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSV 622
            YVN R SQVP S S QVVGWSSFMKGSSLTPSM+NAL ATPASSLAEIEKIYEIA NGS 
Sbjct: 541  YVNTRSSQVPRSASSQVVGWSSFMKGSSLTPSMVNALEATPASSLAEIEKIYEIAINGSG 600

Query: 623  DEKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLV 682
            DEKISAAS+LCGASLVRGWN+QEH VLFI+RLLSPPIPADY G+DSYLIDYA FLN LLV
Sbjct: 601  DEKISAASILCGASLVRGWNLQEHTVLFISRLLSPPIPADYPGSDSYLIDYAPFLNVLLV 660

Query: 683  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTCRAVFSLAFTLL 742
            GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSS PKSWILTSGEELTC AVFSLAFTLL
Sbjct: 661  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSTPKSWILTSGEELTCHAVFSLAFTLL 720

Query: 743  LRLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLKF 802
            LRLWRFHHPP+ENVKGDARPVGSQLTPEYLLLVRN+QLASFG+SPKDRL+ +RLSKLLKF
Sbjct: 721  LRLWRFHHPPIENVKGDARPVGSQLTPEYLLLVRNSQLASFGKSPKDRLKVRRLSKLLKF 780

Query: 803  SVKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLA 862
            S++P +MD+FPKLKGWYRQHQECIASI PGLVPGAPVHQ VDAL+ MMFKKINRGGQSL 
Sbjct: 781  SLEPTFMDSFPKLKGWYRQHQECIASIPPGLVPGAPVHQTVDALLTMMFKKINRGGQSLT 840

Query: 863  STTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDL 922
            STTS SSN+SGSA+EEASIKLKVPAWDIL+ATPFVLDAALTACAHGRLS RDLATGLKDL
Sbjct: 841  STTSASSNSSGSANEEASIKLKVPAWDILEATPFVLDAALTACAHGRLSPRDLATGLKDL 900

Query: 923  ADFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVP 982
            ADFLPA+FATIV YFSAEVTRGIWKPAFMNGTDWPSPAATLS+VEQQIK ILAATGVDVP
Sbjct: 901  ADFLPASFATIVCYFSAEVTRGIWKPAFMNGTDWPSPAATLSVVEQQIKKILAATGVDVP 960

Query: 983  SLAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIAS 1042
            SLA+GG+ PA LPLPLAALISLTITYKLDKASERLLALVGPALNS+ A CSWPCTPIIAS
Sbjct: 961  SLALGGSFPAMLPLPLAALISLTITYKLDKASERLLALVGPALNSLVAGCSWPCTPIIAS 1020

Query: 1043 LWSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGH 1102
            LW+QKVKRWNDFLVFSASRTVFHHN DAVVQLLKSCFTSTLGLGNSNV S GGVG+LLGH
Sbjct: 1021 LWAQKVKRWNDFLVFSASRTVFHHNSDAVVQLLKSCFTSTLGLGNSNVNSGGGVGALLGH 1080

Query: 1103 GFGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKL 1162
            GFGS+VLGGMSPVAPGILYLRVHR +RD+LF+VEEIVS+LMLSV+DIAV+GLPKEKAEKL
Sbjct: 1081 GFGSHVLGGMSPVAPGILYLRVHRCVRDALFLVEEIVSLLMLSVKDIAVTGLPKEKAEKL 1140

Query: 1163 KKAKYGMRYEQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVE 1222
            KK+K+GMR EQVSFASAMARVKLAASLGASLVWI GGSGLVQSL+KETLPSWFLSVHSV+
Sbjct: 1141 KKSKHGMRCEQVSFASAMARVKLAASLGASLVWISGGSGLVQSLYKETLPSWFLSVHSVD 1200

Query: 1223 REGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLASALDGK 1282
            REGVE GGMV VL GYALAFFSVLCGTFSWGIDS SSASKRRAK+LD+HLEFLASALDGK
Sbjct: 1201 REGVEYGGMVPVLRGYALAFFSVLCGTFSWGIDSISSASKRRAKLLDAHLEFLASALDGK 1260

Query: 1283 FSIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESG 1342
            FSIGCDW TWRAYVSGFVSL+VRCAP+WLLEVD+ +L+RL KGLRQLNEEELALALLESG
Sbjct: 1261 FSIGCDWATWRAYVSGFVSLLVRCAPKWLLEVDLKVLERLGKGLRQLNEEELALALLESG 1320

Query: 1343 GVNAMGAAAELIIEGGF 1359
            G+ AMGAAAELIIEGGF
Sbjct: 1321 GLTAMGAAAELIIEGGF 1336

BLAST of Sed0026391 vs. NCBI nr
Match: KAG6571443.1 (Mediator of RNA polymerase II transcription subunit 33A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011209.1 Mediator of RNA polymerase II transcription subunit 33A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2324.7 bits (6023), Expect = 0.0e+00
Identity = 1185/1337 (88.63%), Postives = 1254/1337 (93.79%), Query Frame = 0

Query: 23   MAVSDPPPGQLPVVSGLWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAH 82
            MAVS  PPGQL  ++G+WD VLELTKSAQ KN DPLLWA+QLSS+L+SA VSLPSLELAH
Sbjct: 1    MAVSAQPPGQLQGIAGVWDTVLELTKSAQEKNSDPLLWAVQLSSSLNSASVSLPSLELAH 60

Query: 83   VLVSHICWDNHVPITWKFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLN 142
            +LVSHICWDNHVPI WKFL++AMTA IVPPLLVIALLSTRAIPYRKLR AAYRLYLELL+
Sbjct: 61   LLVSHICWDNHVPIMWKFLEKAMTARIVPPLLVIALLSTRAIPYRKLRPAAYRLYLELLS 120

Query: 143  RHIFSLTLQINGPNYQRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLD 202
            RH+FS TL++NGPNY RIMQTIDDVLHLSQIF LQTCEPGLLMVELFFSIVW LLDASLD
Sbjct: 121  RHVFSSTLEVNGPNYPRIMQTIDDVLHLSQIFDLQTCEPGLLMVELFFSIVWHLLDASLD 180

Query: 203  DEELLVLPAEERSAWLIRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQ 262
            DE LL LPAEERS WLIRPQPHDM+LDVH+S  EK+TEN E LLK+N  KAIE+IGQFLQ
Sbjct: 181  DEGLLELPAEERSVWLIRPQPHDMELDVHDSFGEKKTENSENLLKVNTAKAIEIIGQFLQ 240

Query: 263  NKKTARILCLAQQNMPLHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSR 322
            NKKTARILCLA QNMPLHWAGFA+RLQLLAANSVVL+NTKLIT EVLL WTSDKHR LS+
Sbjct: 241  NKKTARILCLAHQNMPLHWAGFAQRLQLLAANSVVLRNTKLITPEVLLQWTSDKHRFLSQ 300

Query: 323  EGETTSQLVFRDVIMASGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVER 382
            EG+T SQL F DV MASGSLFSSAGQSHGVN SALWLPIDLFLEDAMDGSQVLATSAVER
Sbjct: 301  EGKTKSQLEFHDV-MASGSLFSSAGQSHGVNWSALWLPIDLFLEDAMDGSQVLATSAVER 360

Query: 383  LICLTKSLRAVNDTSWHHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAV 442
            LICL KSLRAVND SWH+TFLGLWIAALRLIQRER+PSEGPVPRLDTCLCMLLSITTLAV
Sbjct: 361  LICLIKSLRAVNDASWHNTFLGLWIAALRLIQRERDPSEGPVPRLDTCLCMLLSITTLAV 420

Query: 443  TIIIEEEECELKEE-ECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVA 502
            TIIIEEEE ELKEE ECSPSKSRDE+QSSGK R+GLITSLQMLGEYESLL PPQSVI VA
Sbjct: 421  TIIIEEEEGELKEEDECSPSKSRDEKQSSGKRRQGLITSLQMLGEYESLLTPPQSVIVVA 480

Query: 503  NQAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPG 562
            NQAAAKAVMFI+GVAVGNEYYDCVSMNDTPI CSGNMRHLIVEACISRNLLDTSAYFWPG
Sbjct: 481  NQAAAKAVMFISGVAVGNEYYDCVSMNDTPINCSGNMRHLIVEACISRNLLDTSAYFWPG 540

Query: 563  YVNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSV 622
            YVN R SQVP S S QVVGWSSFMKGSSLTPSM+NALVATPASSLAEIEKIYEIA NGS 
Sbjct: 541  YVNTRSSQVPRSASSQVVGWSSFMKGSSLTPSMVNALVATPASSLAEIEKIYEIAINGSG 600

Query: 623  DEKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLV 682
            DEKISAAS+LCGASLVRGWN+QEH VLFI+RLLSPPIPADY G+DSYLIDYA FLN LLV
Sbjct: 601  DEKISAASILCGASLVRGWNLQEHTVLFISRLLSPPIPADYPGSDSYLIDYAPFLNVLLV 660

Query: 683  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTCRAVFSLAFTLL 742
            GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSS PKSWILTSGEELTC AVFSLAFTLL
Sbjct: 661  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSTPKSWILTSGEELTCHAVFSLAFTLL 720

Query: 743  LRLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLKF 802
            LRLWRFHHPP+ENVKGDARPVGSQLTPEYLLLVRN+QLASFG+SPKDRL+ +RLSKLLKF
Sbjct: 721  LRLWRFHHPPIENVKGDARPVGSQLTPEYLLLVRNSQLASFGKSPKDRLKVRRLSKLLKF 780

Query: 803  SVKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLA 862
            S++P +MD+FPKLKGWYRQHQECIASI PGLVPGAPVHQ VDAL+ MMFKKINRGGQSL 
Sbjct: 781  SLEPTFMDSFPKLKGWYRQHQECIASIPPGLVPGAPVHQTVDALLTMMFKKINRGGQSLT 840

Query: 863  STTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDL 922
            STTS SSN+SGSA+EEASIKLKVPAWDIL+ATPFVLDAALTACAHGRLS RDLATGLKDL
Sbjct: 841  STTSASSNSSGSANEEASIKLKVPAWDILEATPFVLDAALTACAHGRLSPRDLATGLKDL 900

Query: 923  ADFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVP 982
            ADFLPA+FATIV YFSAEVTRGIWKPAFMNGTDWPSPAATLS+VEQQIK ILAATGVDVP
Sbjct: 901  ADFLPASFATIVCYFSAEVTRGIWKPAFMNGTDWPSPAATLSVVEQQIKKILAATGVDVP 960

Query: 983  SLAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIAS 1042
            SLA+GG+ PA LPLPLAALISLTITYKLDKASERLLALVGPALNS+ A CSWPCTPIIAS
Sbjct: 961  SLALGGSFPAMLPLPLAALISLTITYKLDKASERLLALVGPALNSLVAGCSWPCTPIIAS 1020

Query: 1043 LWSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGH 1102
            LW+QKVKRWNDFLVFSASRTVFHHN DAVVQLLKSCFTSTLGLGNSNV S GGVG+LLGH
Sbjct: 1021 LWAQKVKRWNDFLVFSASRTVFHHNSDAVVQLLKSCFTSTLGLGNSNVNSGGGVGALLGH 1080

Query: 1103 GFGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKL 1162
            GFGS+VLGGMSP APGILYLRVHR +RD+LF+VEEIVS+LMLSV+DIAV+GLPKEKAEKL
Sbjct: 1081 GFGSHVLGGMSPAAPGILYLRVHRCVRDALFLVEEIVSLLMLSVKDIAVTGLPKEKAEKL 1140

Query: 1163 KKAKYGMRYEQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVE 1222
            KK+K+GMR EQVSFASAMARVKLAASLGASLVWI GGSGLVQSL+KETLPSWFLSVHSV+
Sbjct: 1141 KKSKHGMRCEQVSFASAMARVKLAASLGASLVWISGGSGLVQSLYKETLPSWFLSVHSVD 1200

Query: 1223 REGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLASALDGK 1282
            REGVE GGMV VL GYALAFFSVLCGTFSWGIDS SSASKRRAK+LDSHLEFLASALDGK
Sbjct: 1201 REGVEYGGMVPVLRGYALAFFSVLCGTFSWGIDSISSASKRRAKLLDSHLEFLASALDGK 1260

Query: 1283 FSIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESG 1342
            FSIGCDW TWRAYVSGFVSL+VRCAP+WLLEVD+ +LKRL KGLRQLNEEELALALLESG
Sbjct: 1261 FSIGCDWATWRAYVSGFVSLLVRCAPKWLLEVDLKVLKRLGKGLRQLNEEELALALLESG 1320

Query: 1343 GVNAMGAAAELIIEGGF 1359
            G+ AMGAAAELIIEGGF
Sbjct: 1321 GLTAMGAAAELIIEGGF 1336

BLAST of Sed0026391 vs. NCBI nr
Match: XP_022963527.1 (mediator of RNA polymerase II transcription subunit 33B-like [Cucurbita moschata])

HSP 1 Score: 2323.5 bits (6020), Expect = 0.0e+00
Identity = 1184/1337 (88.56%), Postives = 1254/1337 (93.79%), Query Frame = 0

Query: 23   MAVSDPPPGQLPVVSGLWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAH 82
            MAVS  PPGQL  ++G+WD VLELTKSAQ KN DPLLWA+QLSS+L+SA VSLPS+ELAH
Sbjct: 1    MAVSAQPPGQLQGIAGVWDTVLELTKSAQEKNSDPLLWAVQLSSSLNSASVSLPSVELAH 60

Query: 83   VLVSHICWDNHVPITWKFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLN 142
            +LVSHICWDNHVPI WKFL++AMTA IVPPLLVIALLSTRAIPYRKLR AAYRLYLELL+
Sbjct: 61   LLVSHICWDNHVPIMWKFLEKAMTARIVPPLLVIALLSTRAIPYRKLRPAAYRLYLELLS 120

Query: 143  RHIFSLTLQINGPNYQRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLD 202
            RH+FS TL++NGPNY RIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVW LLDASLD
Sbjct: 121  RHVFSSTLEVNGPNYPRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWHLLDASLD 180

Query: 203  DEELLVLPAEERSAWLIRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQ 262
            DE LL LPAEERS WLIRPQPHDM+LDVH+S  EK+TEN E LLK+N  KAIE+IGQFLQ
Sbjct: 181  DEGLLELPAEERSVWLIRPQPHDMELDVHDSFGEKKTENSENLLKVNTAKAIEIIGQFLQ 240

Query: 263  NKKTARILCLAQQNMPLHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSR 322
            NKKTARILCLA QNMPLHWAGFA+RLQLLAANSVVL+NTKLIT EVLL WTSDKHR LS+
Sbjct: 241  NKKTARILCLAHQNMPLHWAGFAQRLQLLAANSVVLRNTKLITPEVLLQWTSDKHRFLSQ 300

Query: 323  EGETTSQLVFRDVIMASGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVER 382
            EG+T SQL F DV MASGSLFSSAGQSHGVN SALWLPIDLFLEDAMDGSQVLATSAVER
Sbjct: 301  EGKTKSQLEFHDV-MASGSLFSSAGQSHGVNWSALWLPIDLFLEDAMDGSQVLATSAVER 360

Query: 383  LICLTKSLRAVNDTSWHHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAV 442
            LICL KSLRAVND SWH+TFLGLWIAALRLIQRER+PSEGPVPRLDTCLCMLLSITTLAV
Sbjct: 361  LICLIKSLRAVNDASWHNTFLGLWIAALRLIQRERDPSEGPVPRLDTCLCMLLSITTLAV 420

Query: 443  TIIIEEEECELKEE-ECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVA 502
            TIIIEEEE ELKEE ECSPSKSRDE+QSSGK R+GLITSLQMLGEYESLL PPQSVI VA
Sbjct: 421  TIIIEEEEGELKEEDECSPSKSRDEKQSSGKRRQGLITSLQMLGEYESLLTPPQSVIVVA 480

Query: 503  NQAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPG 562
            NQAAAKAVMFI+GVAVGNEYYDCVSMNDTPI CSGNMRHLIVEACISRNLLDTSAYFWPG
Sbjct: 481  NQAAAKAVMFISGVAVGNEYYDCVSMNDTPINCSGNMRHLIVEACISRNLLDTSAYFWPG 540

Query: 563  YVNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSV 622
            YVN R SQVP S S QVVGWSSFMKGSSLTPSM+NALVATPASSLAEIEKIYEIA NGS 
Sbjct: 541  YVNTRSSQVPRSASSQVVGWSSFMKGSSLTPSMVNALVATPASSLAEIEKIYEIAINGSG 600

Query: 623  DEKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLV 682
            DEKISAAS+LCGASLVRGWN+QEH VLFI+RLLSPPIPADY G+DSYLIDYA FLN LLV
Sbjct: 601  DEKISAASILCGASLVRGWNLQEHTVLFISRLLSPPIPADYPGSDSYLIDYAPFLNVLLV 660

Query: 683  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTCRAVFSLAFTLL 742
            GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSS PKSWILTSGEELTC AVFSLAFTLL
Sbjct: 661  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSTPKSWILTSGEELTCHAVFSLAFTLL 720

Query: 743  LRLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLKF 802
            LRLWRFHHPP+ENVKGDARPVGSQLTPEYLLLVRN+QLASFG+SPKDRL+ +RLSKLLKF
Sbjct: 721  LRLWRFHHPPIENVKGDARPVGSQLTPEYLLLVRNSQLASFGKSPKDRLKVRRLSKLLKF 780

Query: 803  SVKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLA 862
            S++P +MD+FPKLKGWYRQHQECIASI PGLVPGAPVHQ VDAL+ MMFKKINRGGQSL 
Sbjct: 781  SLEPTFMDSFPKLKGWYRQHQECIASIPPGLVPGAPVHQTVDALLTMMFKKINRGGQSLT 840

Query: 863  STTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDL 922
            STTS SSN+SGSA+EEASIKLKVPAWDIL+ATPFVLDAALTACAHGRLS RDLATGLKDL
Sbjct: 841  STTSASSNSSGSANEEASIKLKVPAWDILEATPFVLDAALTACAHGRLSPRDLATGLKDL 900

Query: 923  ADFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVP 982
            ADFLPA+FATIV YFSAEVTRGIWKPAFMNGTDWPSPAATLS+VEQQIK ILAATGVDVP
Sbjct: 901  ADFLPASFATIVCYFSAEVTRGIWKPAFMNGTDWPSPAATLSVVEQQIKKILAATGVDVP 960

Query: 983  SLAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIAS 1042
            SLA+GG+ PA LPLPLAALISLTITYKLDKASERLLALVGPALNS+ A CSWPCTPIIAS
Sbjct: 961  SLALGGSFPAMLPLPLAALISLTITYKLDKASERLLALVGPALNSLVAGCSWPCTPIIAS 1020

Query: 1043 LWSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGH 1102
            LW+QKVKRWNDFLVFSASRTVFHHN DAVVQLLKSCFTSTLGLGNSNV S GGVG+LLGH
Sbjct: 1021 LWAQKVKRWNDFLVFSASRTVFHHNSDAVVQLLKSCFTSTLGLGNSNVNSGGGVGALLGH 1080

Query: 1103 GFGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKL 1162
            GFGS+VLGGMSP APGILYLRVHR +RD+LF+VEEIVS+LMLSV+DIAV+GLPKEKAEKL
Sbjct: 1081 GFGSHVLGGMSPAAPGILYLRVHRCVRDALFLVEEIVSLLMLSVKDIAVTGLPKEKAEKL 1140

Query: 1163 KKAKYGMRYEQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVE 1222
            KK+K+GMR EQVSFASAMARVKLAASLGASLVWI GGSGLVQSL+KETLPSWFLSVHSV+
Sbjct: 1141 KKSKHGMRCEQVSFASAMARVKLAASLGASLVWISGGSGLVQSLYKETLPSWFLSVHSVD 1200

Query: 1223 REGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLASALDGK 1282
            REGVE GGMV VL GYALAFFSVLCGTFSWGIDS SSASKRRAK+LDSHLEFLASALDGK
Sbjct: 1201 REGVEYGGMVPVLRGYALAFFSVLCGTFSWGIDSISSASKRRAKLLDSHLEFLASALDGK 1260

Query: 1283 FSIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESG 1342
            FSIGCDW TWRAYVSGFVSL+VRCAP+WLLEVD+ +LKRL KGLRQLNEEELALALLESG
Sbjct: 1261 FSIGCDWATWRAYVSGFVSLLVRCAPKWLLEVDLKVLKRLGKGLRQLNEEELALALLESG 1320

Query: 1343 GVNAMGAAAELIIEGGF 1359
            G+ AMGAAAELII GGF
Sbjct: 1321 GLTAMGAAAELIIGGGF 1336

BLAST of Sed0026391 vs. ExPASy Swiss-Prot
Match: Q9LUG9 (Mediator of RNA polymerase II transcription subunit 33A OS=Arabidopsis thaliana OX=3702 GN=MED33A PE=1 SV=1)

HSP 1 Score: 1457.2 bits (3771), Expect = 0.0e+00
Identity = 767/1319 (58.15%), Postives = 973/1319 (73.77%), Query Frame = 0

Query: 39   LWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAHVLVSHICWDNHVPITW 98
            +WD V+ELTK AQ    DP LWA QLSS L    V LPS ELA V+VS+ICWDN+VPI W
Sbjct: 9    VWDCVIELTKMAQENCVDPRLWASQLSSNLKFFAVELPSTELAEVIVSYICWDNNVPIVW 68

Query: 99   KFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLNRHIFSLTLQINGPNYQ 158
            KFL++AM   +V PL+V+ALL+ R +P R  + AAYR+YLELL R++F++   I+GP+YQ
Sbjct: 69   KFLERAMALKLVSPLVVLALLADRVVPTRSTQQAAYRIYLELLKRNMFTIKDHISGPHYQ 128

Query: 159  RIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLDDEELLVLPAEERSAWL 218
            ++M ++ ++L LS++F L T +PG+L+VE  F +V QLLDA+L DE LL L  +  S WL
Sbjct: 129  KVMISVSNILRLSELFDLDTSKPGVLLVEFVFKMVSQLLDAALSDEGLLELSQDSSSQWL 188

Query: 219  IRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQNKKTARILCLAQQNMP 278
            ++ Q  DM++D  E  +EK T + E+L  +N   AIELI +FL+N   AR+L L   N  
Sbjct: 189  VKSQ--DMEIDAPERYNEK-TGSLEKLQSLNTIMAIELIAEFLRNTVIARLLYLVSSNRA 248

Query: 279  LHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSREGETTSQLVFRDVIMA 338
              W  F +++QLL  NS  LK++K++ S  LL   S++    S + + TS     + I+ 
Sbjct: 249  SKWHEFVQKVQLLGENSSALKHSKVLNSGDLLQLISNRRFGYSYDSKVTSARK-SNAIVD 308

Query: 339  SGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVERLICLTKSLRAVNDTSW 398
             GSL S AG  HG +LS+LWLP+DL  EDAMDG QV  TSA+E +  L K+L+ +N ++W
Sbjct: 309  FGSLSSYAGLCHGASLSSLWLPLDLVFEDAMDGYQVNPTSAIEIITGLAKTLKEINGSTW 368

Query: 399  HHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAVTIIIEEEECELKEEEC 458
            H TFLGLWIAALRL+QRER+P EGP+PRLDT LCM L I  L V  +IEE + E   E  
Sbjct: 369  HDTFLGLWIAALRLVQRERDPIEGPIPRLDTRLCMSLCIVPLVVANLIEEGKYESVME-- 428

Query: 459  SPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVANQAAAKAVMFIAGVAVG 518
                         K R  L+TSLQ+LG++  LLAPP+ V++ AN+AA KA++F++G  VG
Sbjct: 429  -------------KLRDDLVTSLQVLGDFPGLLAPPKCVVSAANKAATKAILFLSGGNVG 488

Query: 519  NEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPGYVNARKSQVPSSTSGQV 578
               +D ++M D P+ CSGNMRHLIVEACI+RN+LD SAY WPGYVN R +Q+P S   +V
Sbjct: 489  KSCFDVINMKDMPVNCSGNMRHLIVEACIARNILDMSAYSWPGYVNGRINQIPQSLPNEV 548

Query: 579  VGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSVDEKISAASVLCGASLVR 638
              WSSF+KG+ L  +M+N LV+ PASSLAE+EK++E+A  GS DEKISAA+VLCGASL R
Sbjct: 549  PCWSSFVKGAPLNAAMVNTLVSVPASSLAELEKLFEVAVKGSDDEKISAATVLCGASLTR 608

Query: 639  GWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLVGISSVDCVQIFSLHGMV 698
            GWNIQEH V ++ RLLSPP+PADYS  +++LI YA  LN ++VGI SVD +QIFSLHGMV
Sbjct: 609  GWNIQEHTVEYLTRLLSPPVPADYSRAENHLIGYACMLNVVIVGIGSVDSIQIFSLHGMV 668

Query: 699  PLLAGQLMPICEAFGS-SPPKSWILTSGEELTCRAVFSLAFTLLLRLWRFHHPPVENVKG 758
            P LA  LMPICE FGS +P  SW L SGE ++  +VFS AFTLLL+LWRF+HPP+E+  G
Sbjct: 669  PQLACSLMPICEEFGSYTPSVSWTLPSGEAISAYSVFSNAFTLLLKLWRFNHPPIEHGVG 728

Query: 759  DARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLK-FSVKPVYMDAFPKLKG 818
            D   VGSQLTPE+LL VRN+ L S     +DR R KRLS++ +  S +PV++D+FPKLK 
Sbjct: 729  DVPTVGSQLTPEHLLSVRNSYLVSSEILDRDRNR-KRLSEVARAASCQPVFVDSFPKLKV 788

Query: 819  WYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLASTTSGSSNTSGSASE 878
            WYRQHQ CIA+ L GL  G+PVHQ V+AL+ M F K+ RG Q+L    SG+S++SG+ASE
Sbjct: 789  WYRQHQRCIAATLSGLTHGSPVHQTVEALLNMTFGKV-RGSQTLNPVNSGTSSSSGAASE 848

Query: 879  EASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDLADFLPATFATIVSYF 938
            +++I+ + PAWDILKA P+V+DAALTAC HGRLS R LATGLKDLADFLPA+ ATIVSYF
Sbjct: 849  DSNIRPEFPAWDILKAVPYVVDAALTACTHGRLSPRQLATGLKDLADFLPASLATIVSYF 908

Query: 939  SAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVPSLAVGGTSPATLPLP 998
            SAEV+RG+WKP FMNG DWPSPA  LS VE+ I  ILA TGVD+PSLA GG+SPATLPLP
Sbjct: 909  SAEVSRGVWKPVFMNGVDWPSPATNLSTVEEYITKILATTGVDIPSLAPGGSSPATLPLP 968

Query: 999  LAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIASLWSQKVKRWNDFLVF 1058
            LAA +SLTITYK+DKASER L L GPAL  +AA C WPC PI+ASLW+QK KRW DFLVF
Sbjct: 969  LAAFVSLTITYKIDKASERFLNLAGPALECLAAGCPWPCMPIVASLWTQKAKRWFDFLVF 1028

Query: 1059 SASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGHGFGSNVLGGMSPVAP 1118
            SASRTVF HN DAV+QLL++CF++TLGL  + + + GGVG+LLGHGFGS+  GG+SPVAP
Sbjct: 1029 SASRTVFLHNQDAVIQLLRNCFSATLGLNAAPMSNDGGVGALLGHGFGSHFYGGISPVAP 1088

Query: 1119 GILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKLKKAKYGMRYEQVSFA 1178
            GILYLR++R++RD++ + EEI+S+L+ SV DIA + L KEK EKLK  K G RY Q S A
Sbjct: 1089 GILYLRMYRALRDTVSVSEEILSLLIHSVEDIAQNRLSKEKLEKLKTVKNGSRYGQSSLA 1148

Query: 1179 SAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVEREGVECGGMVAVLGG 1238
            +AM +VKLAASL ASLVW+ GG G+V  L KET+PSWFLS    +RE      +VA L G
Sbjct: 1149 TAMTQVKLAASLSASLVWLTGGLGVVHVLIKETIPSWFLSTDKSDREQGP-SDLVAELRG 1208

Query: 1239 YALAFFSVLCGTFSWGIDSASSASKRRAK-ILDSHLEFLASALDGKFSIGCDWGTWRAYV 1298
            +ALA+F VLCG  +WG+DS SSASKRR + IL SHLEF+ASALDGK S+GC+  TWR Y+
Sbjct: 1209 HALAYFVVLCGALTWGVDSRSSASKRRRQAILGSHLEFIASALDGKISVGCETATWRTYI 1268

Query: 1299 SGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESGGVNAMGAAAELII 1355
            SG VSLMV C P W+ E+D  +LK LS GLR+  ++ELA+ LL  GG+  M  AA+ II
Sbjct: 1269 SGLVSLMVSCLPLWVTEIDTEVLKSLSNGLRKWGKDELAIVLLSLGGLKTMDYAADFII 1305

BLAST of Sed0026391 vs. ExPASy Swiss-Prot
Match: F4IN69 (Mediator of RNA polymerase II transcription subunit 33B OS=Arabidopsis thaliana OX=3702 GN=MED33B PE=1 SV=1)

HSP 1 Score: 1446.4 bits (3743), Expect = 0.0e+00
Identity = 790/1340 (58.96%), Postives = 953/1340 (71.12%), Query Frame = 0

Query: 39   LWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAHVLVSHICWDNHVPITW 98
            LW+ V  L +SAQ KN DPL WALQL  TL SA +SLPS +LA  LV+HI W+NH P++W
Sbjct: 10   LWESVTSLIRSAQEKNVDPLHWALQLRLTLASAGISLPSPDLAQFLVTHIFWENHSPLSW 69

Query: 99   KFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLNRHIFSLTLQINGPNYQ 158
            K L++A++ NIVPPLLV+ALLS R IP RKL  AAYRLY+ELL RH FS    I  P Y 
Sbjct: 70   KLLEKAISVNIVPPLLVLALLSPRVIPNRKLHPAAYRLYMELLKRHAFSFMPLIRAPGYH 129

Query: 159  RIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLDDEELLVLPAEERSAWL 218
            + M +IDD+LHLS+ FG+Q  EPG +++   FSIVW+LLDASLD+E LL L + +RS W 
Sbjct: 130  KTMNSIDDILHLSETFGVQDQEPGSILLAFVFSIVWELLDASLDEEGLLELTSNKRSKW- 189

Query: 219  IRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQNKKTARILCLAQQNMP 278
                PHDM LD  E+ S KR EN + L K N E AIELI +FLQNK T+RIL LA QNM 
Sbjct: 190  -PSSPHDMDLDGLEN-SVKRNENHDALEKANTEMAIELIQEFLQNKVTSRILHLASQNM- 249

Query: 279  LHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSREGETTSQLVFRDVIMA 338
                                                        E +T  +  F   I++
Sbjct: 250  --------------------------------------------ESKTIPRGEFH-AIVS 309

Query: 339  SGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVERLICLTKSLRAVNDTSW 398
            SGS  +          SALWLPIDLF ED MDG+Q  A SAVE L  L K+L+A N TSW
Sbjct: 310  SGSKLALTSD------SALWLPIDLFFEDIMDGTQAAAASAVENLTGLVKALQAANSTSW 369

Query: 399  HHTFLGLWIAALRLIQR-------------------ERNPSEGPVPRLDTCLCMLLSITT 458
            H  FL LW+AALRL+QR                   ER+P EGPVPR DT LC+LLS+T 
Sbjct: 370  HDAFLALWLAALRLVQRENLCLRYCFFMHMLEILSEERDPIEGPVPRTDTFLCVLLSVTP 429

Query: 459  LAVTIIIEEEECELKEEECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIA 518
            LAV  IIEEEE +   ++ S S S   ++  GKCR+GLI SLQ LG+YESLL PP+SV +
Sbjct: 430  LAVANIIEEEESQW-IDQTSSSPSNQWKEKKGKCRQGLINSLQQLGDYESLLTPPRSVQS 489

Query: 519  VANQAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFW 578
            VANQAAAKA+MFI+G+   N  Y+  SM+++   C           C  R  L T   F 
Sbjct: 490  VANQAAAKAIMFISGITNSNGSYENTSMSESASGC-----------CKVRFSLFTLKMFV 549

Query: 579  PGYVNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENG 638
               V    +         +  WS  MKGS LTPS+ N+L+ TPASSLAEIEK+YE+A  G
Sbjct: 550  VMGVYLLCN---------ISCWSLVMKGSPLTPSLTNSLITTPASSLAEIEKMYEVATTG 609

Query: 639  SVDEKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNAL 698
            S DEKI+ AS+LCGASL RGW+IQEH+++FI  LLSPP PAD SG+ S+LI+ A FLN L
Sbjct: 610  SEDEKIAVASILCGASLFRGWSIQEHVIIFIVTLLSPPAPADLSGSYSHLINSAPFLNVL 669

Query: 699  LVGISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPK-SWILTSGEELTCRAVFSLAF 758
            LVGIS +DCV IFSLHG+VPLLAG LMPICEAFGS  P  +W L +GE ++  AVFS AF
Sbjct: 670  LVGISPIDCVHIFSLHGVVPLLAGALMPICEAFGSGVPNITWTLPTGELISSHAVFSTAF 729

Query: 759  TLLLRLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKL 818
            TLLLRLWRF HPP++ V GD  PVG Q +PEYLLLVRN +L  FG+SPKDR+  +R SK+
Sbjct: 730  TLLLRLWRFDHPPLDYVLGDVPPVGPQPSPEYLLLVRNCRLECFGKSPKDRMARRRFSKV 789

Query: 819  LKFSVKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQ 878
            +  SV P++MD+FP+LK WYRQHQEC+ASIL  L  G+PVH IVD+L+ MMFKK N+GG 
Sbjct: 790  IDISVDPIFMDSFPRLKQWYRQHQECMASILSELKTGSPVHHIVDSLLSMMFKKANKGGS 849

Query: 879  SLASTTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGL 938
               + +SGSS+ S S  +++S +LK+PAWDIL+A PFVLDAALTACAHG LS R+LATGL
Sbjct: 850  QSLTPSSGSSSLSTSGGDDSSDQLKLPAWDILEAAPFVLDAALTACAHGSLSPRELATGL 909

Query: 939  KDLADFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGV 998
            K LADFLPAT  T+VSYFS+EVTRG+WKP  MNGTDWPSPAA L+ VEQQI+ ILAATGV
Sbjct: 910  KILADFLPATLGTMVSYFSSEVTRGLWKPVSMNGTDWPSPAANLASVEQQIEKILAATGV 969

Query: 999  DVPSLAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPI 1058
            DVP L   G S ATLPLPLAAL+SLTITYKLDKA+ER L LVGPAL+S+AA+C WPC PI
Sbjct: 970  DVPRLPADGISAATLPLPLAALVSLTITYKLDKATERFLVLVGPALDSLAAACPWPCMPI 1029

Query: 1059 IASLWSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGL-GNSNVFSSGGVGS 1118
            + SLW+QKVKRW+DFL+FSASRTVFHHN DAV+QLL+SCFT TLGL   S + S GGVG+
Sbjct: 1030 VTSLWTQKVKRWSDFLIFSASRTVFHHNRDAVIQLLRSCFTCTLGLTPTSQLCSYGGVGA 1089

Query: 1119 LLGHGFGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEK 1178
            LLGHGFGS   GG+S  APGILY++VHRS+RD +F+ EEI+S+LM SV+ IA   LP  +
Sbjct: 1090 LLGHGFGSRYSGGISTAAPGILYIKVHRSIRDVMFLTEEILSLLMFSVKSIATRELPAGQ 1149

Query: 1179 AEKLKKAKYGMRY--EQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFL 1238
            AEKLKK K G RY   QVS + AM RVKLAASLGASLVWI GG  LVQ+L KETLPSWF+
Sbjct: 1150 AEKLKKTKDGSRYGIGQVSLSLAMRRVKLAASLGASLVWISGGLNLVQALIKETLPSWFI 1209

Query: 1239 SVHSVEREGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLA 1298
            SVH  E    E GGMV +L GYALA+F++L   F+WG+DS+  ASKRR ++L  HLEF+ 
Sbjct: 1210 SVHGEED---ELGGMVPMLRGYALAYFAILSSAFAWGVDSSYPASKRRPRVLWLHLEFMV 1269

Query: 1299 SALDGKFSIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELAL 1356
            SAL+GK S+GCDW TW+AYV+GFVSLMV+C P W+LEVDV ++KRLSK LRQ NE++LAL
Sbjct: 1270 SALEGKISLGCDWATWQAYVTGFVSLMVQCTPAWVLEVDVEVIKRLSKSLRQWNEQDLAL 1270

BLAST of Sed0026391 vs. ExPASy TrEMBL
Match: A0A6J1DPP9 (mediator of RNA polymerase II transcription subunit 33B-like OS=Momordica charantia OX=3673 GN=LOC111022676 PE=4 SV=1)

HSP 1 Score: 2330.1 bits (6037), Expect = 0.0e+00
Identity = 1190/1337 (89.01%), Postives = 1261/1337 (94.32%), Query Frame = 0

Query: 23   MAVSDPPPGQLPVVSGLWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAH 82
            M VS  PP QL  ++GLWD VLELTKSAQ KN DPLLWA+QLSS+L+SA VSLPS+ELA 
Sbjct: 1    MVVSVQPPSQLQGMAGLWDSVLELTKSAQDKNCDPLLWAVQLSSSLNSAGVSLPSIELAQ 60

Query: 83   VLVSHICWDNHVPITWKFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLN 142
            +LVSHICWDNHVPI WKFL++AMTA IVPPLLV+ALLSTRAIPYRKLR AAYRLYLELL+
Sbjct: 61   LLVSHICWDNHVPIMWKFLEKAMTAKIVPPLLVVALLSTRAIPYRKLRPAAYRLYLELLS 120

Query: 143  RHIFSLTLQINGPNYQRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLD 202
            RH+FS T QINGPNYQRIMQTIDDVLHLSQIF LQ CEPGLLMVELFFSIVWQLLDASLD
Sbjct: 121  RHVFSSTSQINGPNYQRIMQTIDDVLHLSQIFSLQACEPGLLMVELFFSIVWQLLDASLD 180

Query: 203  DEELLVLPAEERSAWLIRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQ 262
            DE LLVLPAEERSAWLIRPQPHDM+LDVH+S SEKRTEN E LLK+N  KAIE+IGQFLQ
Sbjct: 181  DEGLLVLPAEERSAWLIRPQPHDMELDVHDSFSEKRTENSESLLKVNTAKAIEIIGQFLQ 240

Query: 263  NKKTARILCLAQQNMPLHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSR 322
            NKKTARIL LA +NMPLHWAGFA+RLQLLAANS VL+NTKLIT EVLLHWTSDKHRLLSR
Sbjct: 241  NKKTARILYLAHRNMPLHWAGFAQRLQLLAANSAVLRNTKLITPEVLLHWTSDKHRLLSR 300

Query: 323  EGETTSQLVFRDVIMASGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVER 382
            EG+ TSQ  FR+V MASGSLFSSAGQSHGVN S LWLPIDLFLEDAMDGSQVLATSAVER
Sbjct: 301  EGK-TSQQEFRNV-MASGSLFSSAGQSHGVNWSTLWLPIDLFLEDAMDGSQVLATSAVER 360

Query: 383  LICLTKSLRAVNDTSWHHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAV 442
            LICL KSL+AVNDTSWH+TF+GLWIAALRLIQRER+PSEGPVPRLDTCLCMLLSITTLAV
Sbjct: 361  LICLIKSLQAVNDTSWHNTFMGLWIAALRLIQRERDPSEGPVPRLDTCLCMLLSITTLAV 420

Query: 443  TIIIEEEECELKEE-ECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVA 502
            TIIIEE+E ELKEE ECSPSK RDE++ SGKCRKGLITSLQMLGEYE LL PPQSV A+A
Sbjct: 421  TIIIEEDEGELKEEDECSPSKGRDEKKCSGKCRKGLITSLQMLGEYEGLLTPPQSVTAIA 480

Query: 503  NQAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPG 562
            NQAAAKAVMFI+GVAVGNEYYDCVSMNDTP+ CSGNMRHLIVEACISRNLLDTS YFWPG
Sbjct: 481  NQAAAKAVMFISGVAVGNEYYDCVSMNDTPVNCSGNMRHLIVEACISRNLLDTSVYFWPG 540

Query: 563  YVNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSV 622
            YVNAR SQVP S SGQVVGWSSFMKGSSLT SM++ALVATPASSLAEIEKIYEIA NGS 
Sbjct: 541  YVNARSSQVPRSASGQVVGWSSFMKGSSLTLSMVDALVATPASSLAEIEKIYEIAVNGSG 600

Query: 623  DEKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLV 682
            DEKISAAS+LCG SLVRGWN+QEH VLFIARLLSPPIPADYSG+DSYLIDYA FLN LLV
Sbjct: 601  DEKISAASILCGESLVRGWNLQEHTVLFIARLLSPPIPADYSGSDSYLIDYAPFLNVLLV 660

Query: 683  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTCRAVFSLAFTLL 742
            GISSVDCVQIFSLHGMVPLLAGQLMPICEAFG SPPKSW+LTSGEELTC AVFSLAFTLL
Sbjct: 661  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGLSPPKSWVLTSGEELTCHAVFSLAFTLL 720

Query: 743  LRLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLKF 802
            LRLWRFHHPPVENVK DARPVGSQLTPEYLLLVRN+QLASFG+SPKDR + +RLSKLLKF
Sbjct: 721  LRLWRFHHPPVENVKRDARPVGSQLTPEYLLLVRNSQLASFGKSPKDRFKVRRLSKLLKF 780

Query: 803  SVKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLA 862
            S++P++MD+FPKLKGWYRQHQECIASIL GLVPGAPVHQIVDAL+ MMF+KINRGG SL 
Sbjct: 781  SLEPIFMDSFPKLKGWYRQHQECIASILSGLVPGAPVHQIVDALLTMMFRKINRGGHSLT 840

Query: 863  STTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDL 922
            STTSGSSN+SGSA+EEASIKLKVPAWDIL+ATPFVLDAALTACAHGRLS RDLATGLKDL
Sbjct: 841  STTSGSSNSSGSANEEASIKLKVPAWDILEATPFVLDAALTACAHGRLSPRDLATGLKDL 900

Query: 923  ADFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVP 982
            ADFLPA+FATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIK ILAATGVDVP
Sbjct: 901  ADFLPASFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKKILAATGVDVP 960

Query: 983  SLAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIAS 1042
            SLAVGG SPA LPLPLAALISLTITYKLDKASERLLALVGPALN++AASCSWPCTPIIAS
Sbjct: 961  SLAVGGNSPAMLPLPLAALISLTITYKLDKASERLLALVGPALNTLAASCSWPCTPIIAS 1020

Query: 1043 LWSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGH 1102
            LW+QKVKRWNDFLVFSASRTVFHHN DAVVQLLKSCFTSTLGLGNSN+ S+GGVG+LLGH
Sbjct: 1021 LWAQKVKRWNDFLVFSASRTVFHHNSDAVVQLLKSCFTSTLGLGNSNLNSNGGVGTLLGH 1080

Query: 1103 GFGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKL 1162
            GFGS+VLGGMSPVAPGILYLRVHRS+RD+LFMVEEIVS+LMLSVRDIAVSGLP+EKAEKL
Sbjct: 1081 GFGSHVLGGMSPVAPGILYLRVHRSVRDALFMVEEIVSLLMLSVRDIAVSGLPREKAEKL 1140

Query: 1163 KKAKYGMRYEQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVE 1222
            KK K+GMRYEQVSFASAM+RVKLAASLGASLVWI GGSGLVQSLFKETLPSWFLSVHS+E
Sbjct: 1141 KKTKHGMRYEQVSFASAMSRVKLAASLGASLVWISGGSGLVQSLFKETLPSWFLSVHSLE 1200

Query: 1223 REGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLASALDGK 1282
            REGVE GGMVAVLGGYALAFFSVLCGTFSWGIDS SSASKRRAKILDSHLEFLASALDGK
Sbjct: 1201 REGVEYGGMVAVLGGYALAFFSVLCGTFSWGIDSVSSASKRRAKILDSHLEFLASALDGK 1260

Query: 1283 FSIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESG 1342
            FSIGCDW TWRAYVSGFVSLMVRCAP+W++EVDVN+LKRLS GLRQL+EEELALALLESG
Sbjct: 1261 FSIGCDWATWRAYVSGFVSLMVRCAPKWVVEVDVNILKRLSNGLRQLSEEELALALLESG 1320

Query: 1343 GVNAMGAAAELIIEGGF 1359
            GV AMGAAAELIIEGGF
Sbjct: 1321 GVTAMGAAAELIIEGGF 1335

BLAST of Sed0026391 vs. ExPASy TrEMBL
Match: A0A6J1HFH4 (mediator of RNA polymerase II transcription subunit 33B-like OS=Cucurbita moschata OX=3662 GN=LOC111463830 PE=4 SV=1)

HSP 1 Score: 2323.5 bits (6020), Expect = 0.0e+00
Identity = 1184/1337 (88.56%), Postives = 1254/1337 (93.79%), Query Frame = 0

Query: 23   MAVSDPPPGQLPVVSGLWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAH 82
            MAVS  PPGQL  ++G+WD VLELTKSAQ KN DPLLWA+QLSS+L+SA VSLPS+ELAH
Sbjct: 1    MAVSAQPPGQLQGIAGVWDTVLELTKSAQEKNSDPLLWAVQLSSSLNSASVSLPSVELAH 60

Query: 83   VLVSHICWDNHVPITWKFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLN 142
            +LVSHICWDNHVPI WKFL++AMTA IVPPLLVIALLSTRAIPYRKLR AAYRLYLELL+
Sbjct: 61   LLVSHICWDNHVPIMWKFLEKAMTARIVPPLLVIALLSTRAIPYRKLRPAAYRLYLELLS 120

Query: 143  RHIFSLTLQINGPNYQRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLD 202
            RH+FS TL++NGPNY RIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVW LLDASLD
Sbjct: 121  RHVFSSTLEVNGPNYPRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWHLLDASLD 180

Query: 203  DEELLVLPAEERSAWLIRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQ 262
            DE LL LPAEERS WLIRPQPHDM+LDVH+S  EK+TEN E LLK+N  KAIE+IGQFLQ
Sbjct: 181  DEGLLELPAEERSVWLIRPQPHDMELDVHDSFGEKKTENSENLLKVNTAKAIEIIGQFLQ 240

Query: 263  NKKTARILCLAQQNMPLHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSR 322
            NKKTARILCLA QNMPLHWAGFA+RLQLLAANSVVL+NTKLIT EVLL WTSDKHR LS+
Sbjct: 241  NKKTARILCLAHQNMPLHWAGFAQRLQLLAANSVVLRNTKLITPEVLLQWTSDKHRFLSQ 300

Query: 323  EGETTSQLVFRDVIMASGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVER 382
            EG+T SQL F DV MASGSLFSSAGQSHGVN SALWLPIDLFLEDAMDGSQVLATSAVER
Sbjct: 301  EGKTKSQLEFHDV-MASGSLFSSAGQSHGVNWSALWLPIDLFLEDAMDGSQVLATSAVER 360

Query: 383  LICLTKSLRAVNDTSWHHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAV 442
            LICL KSLRAVND SWH+TFLGLWIAALRLIQRER+PSEGPVPRLDTCLCMLLSITTLAV
Sbjct: 361  LICLIKSLRAVNDASWHNTFLGLWIAALRLIQRERDPSEGPVPRLDTCLCMLLSITTLAV 420

Query: 443  TIIIEEEECELKEE-ECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVA 502
            TIIIEEEE ELKEE ECSPSKSRDE+QSSGK R+GLITSLQMLGEYESLL PPQSVI VA
Sbjct: 421  TIIIEEEEGELKEEDECSPSKSRDEKQSSGKRRQGLITSLQMLGEYESLLTPPQSVIVVA 480

Query: 503  NQAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPG 562
            NQAAAKAVMFI+GVAVGNEYYDCVSMNDTPI CSGNMRHLIVEACISRNLLDTSAYFWPG
Sbjct: 481  NQAAAKAVMFISGVAVGNEYYDCVSMNDTPINCSGNMRHLIVEACISRNLLDTSAYFWPG 540

Query: 563  YVNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSV 622
            YVN R SQVP S S QVVGWSSFMKGSSLTPSM+NALVATPASSLAEIEKIYEIA NGS 
Sbjct: 541  YVNTRSSQVPRSASSQVVGWSSFMKGSSLTPSMVNALVATPASSLAEIEKIYEIAINGSG 600

Query: 623  DEKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLV 682
            DEKISAAS+LCGASLVRGWN+QEH VLFI+RLLSPPIPADY G+DSYLIDYA FLN LLV
Sbjct: 601  DEKISAASILCGASLVRGWNLQEHTVLFISRLLSPPIPADYPGSDSYLIDYAPFLNVLLV 660

Query: 683  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTCRAVFSLAFTLL 742
            GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSS PKSWILTSGEELTC AVFSLAFTLL
Sbjct: 661  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSTPKSWILTSGEELTCHAVFSLAFTLL 720

Query: 743  LRLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLKF 802
            LRLWRFHHPP+ENVKGDARPVGSQLTPEYLLLVRN+QLASFG+SPKDRL+ +RLSKLLKF
Sbjct: 721  LRLWRFHHPPIENVKGDARPVGSQLTPEYLLLVRNSQLASFGKSPKDRLKVRRLSKLLKF 780

Query: 803  SVKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLA 862
            S++P +MD+FPKLKGWYRQHQECIASI PGLVPGAPVHQ VDAL+ MMFKKINRGGQSL 
Sbjct: 781  SLEPTFMDSFPKLKGWYRQHQECIASIPPGLVPGAPVHQTVDALLTMMFKKINRGGQSLT 840

Query: 863  STTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDL 922
            STTS SSN+SGSA+EEASIKLKVPAWDIL+ATPFVLDAALTACAHGRLS RDLATGLKDL
Sbjct: 841  STTSASSNSSGSANEEASIKLKVPAWDILEATPFVLDAALTACAHGRLSPRDLATGLKDL 900

Query: 923  ADFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVP 982
            ADFLPA+FATIV YFSAEVTRGIWKPAFMNGTDWPSPAATLS+VEQQIK ILAATGVDVP
Sbjct: 901  ADFLPASFATIVCYFSAEVTRGIWKPAFMNGTDWPSPAATLSVVEQQIKKILAATGVDVP 960

Query: 983  SLAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIAS 1042
            SLA+GG+ PA LPLPLAALISLTITYKLDKASERLLALVGPALNS+ A CSWPCTPIIAS
Sbjct: 961  SLALGGSFPAMLPLPLAALISLTITYKLDKASERLLALVGPALNSLVAGCSWPCTPIIAS 1020

Query: 1043 LWSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGH 1102
            LW+QKVKRWNDFLVFSASRTVFHHN DAVVQLLKSCFTSTLGLGNSNV S GGVG+LLGH
Sbjct: 1021 LWAQKVKRWNDFLVFSASRTVFHHNSDAVVQLLKSCFTSTLGLGNSNVNSGGGVGALLGH 1080

Query: 1103 GFGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKL 1162
            GFGS+VLGGMSP APGILYLRVHR +RD+LF+VEEIVS+LMLSV+DIAV+GLPKEKAEKL
Sbjct: 1081 GFGSHVLGGMSPAAPGILYLRVHRCVRDALFLVEEIVSLLMLSVKDIAVTGLPKEKAEKL 1140

Query: 1163 KKAKYGMRYEQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVE 1222
            KK+K+GMR EQVSFASAMARVKLAASLGASLVWI GGSGLVQSL+KETLPSWFLSVHSV+
Sbjct: 1141 KKSKHGMRCEQVSFASAMARVKLAASLGASLVWISGGSGLVQSLYKETLPSWFLSVHSVD 1200

Query: 1223 REGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLASALDGK 1282
            REGVE GGMV VL GYALAFFSVLCGTFSWGIDS SSASKRRAK+LDSHLEFLASALDGK
Sbjct: 1201 REGVEYGGMVPVLRGYALAFFSVLCGTFSWGIDSISSASKRRAKLLDSHLEFLASALDGK 1260

Query: 1283 FSIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESG 1342
            FSIGCDW TWRAYVSGFVSL+VRCAP+WLLEVD+ +LKRL KGLRQLNEEELALALLESG
Sbjct: 1261 FSIGCDWATWRAYVSGFVSLLVRCAPKWLLEVDLKVLKRLGKGLRQLNEEELALALLESG 1320

Query: 1343 GVNAMGAAAELIIEGGF 1359
            G+ AMGAAAELII GGF
Sbjct: 1321 GLTAMGAAAELIIGGGF 1336

BLAST of Sed0026391 vs. ExPASy TrEMBL
Match: A0A6J1HUY1 (mediator of RNA polymerase II transcription subunit 33B-like OS=Cucurbita maxima OX=3661 GN=LOC111466930 PE=4 SV=1)

HSP 1 Score: 2312.3 bits (5991), Expect = 0.0e+00
Identity = 1178/1337 (88.11%), Postives = 1251/1337 (93.57%), Query Frame = 0

Query: 23   MAVSDPPPGQLPVVSGLWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAH 82
            MAVS  PPGQL  ++G+WD VLELTKSAQ KN DPLLWA+ LSS+L+SA VSLPS+ELA 
Sbjct: 1    MAVSAQPPGQLQGIAGVWDTVLELTKSAQEKNSDPLLWAVHLSSSLNSASVSLPSVELAQ 60

Query: 83   VLVSHICWDNHVPITWKFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLN 142
            +LVSHICWDNHVPI WKFL++AMTA IVPPLLVIALLSTRAIPYRKLR AAYRLYLELL+
Sbjct: 61   LLVSHICWDNHVPIMWKFLEKAMTARIVPPLLVIALLSTRAIPYRKLRPAAYRLYLELLS 120

Query: 143  RHIFSLTLQINGPNYQRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLD 202
            RH+FS TL++NGPNY RIMQTIDDVLHLSQIFGLQTCEPGLL+VELFFSIVW LLDASLD
Sbjct: 121  RHVFSSTLEVNGPNYPRIMQTIDDVLHLSQIFGLQTCEPGLLVVELFFSIVWHLLDASLD 180

Query: 203  DEELLVLPAEERSAWLIRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQ 262
            DE LL LPAEERS WLIRPQPH+M+LDVH S  EK+TEN E LLK+N  KAIE+IGQFLQ
Sbjct: 181  DEGLLELPAEERSVWLIRPQPHNMELDVHNSFGEKKTENSENLLKVNTAKAIEIIGQFLQ 240

Query: 263  NKKTARILCLAQQNMPLHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSR 322
            NKKTARILCLA QNMPLHWAGFA+RLQLLAANSVVL+NTKLIT EVLLHWTSDKHR LS+
Sbjct: 241  NKKTARILCLAHQNMPLHWAGFAQRLQLLAANSVVLRNTKLITPEVLLHWTSDKHRFLSQ 300

Query: 323  EGETTSQLVFRDVIMASGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVER 382
            EG+T SQL F DV MASGSLFSSAGQSHGVN SALWLPIDLFLEDAMDGSQVLATSAVER
Sbjct: 301  EGKTASQLEFHDV-MASGSLFSSAGQSHGVNWSALWLPIDLFLEDAMDGSQVLATSAVER 360

Query: 383  LICLTKSLRAVNDTSWHHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAV 442
            LICL KSLRAVND SWH+TFLGLWIAALRLIQRER+PSEGPVPRLDTCLCMLLSITTLAV
Sbjct: 361  LICLIKSLRAVNDASWHNTFLGLWIAALRLIQRERDPSEGPVPRLDTCLCMLLSITTLAV 420

Query: 443  TIIIEEEECELKEE-ECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVA 502
            TIIIEEEE ELKEE ECSPSKSRDE+QSSGK R+GLIT LQMLGEYESLL PPQSVI VA
Sbjct: 421  TIIIEEEEGELKEEDECSPSKSRDEKQSSGKRRQGLITCLQMLGEYESLLTPPQSVIEVA 480

Query: 503  NQAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPG 562
            NQAAAKAVMFI+GVAVGNE YDCVSMNDTPI CSGNMRHLIVEACISRNLLDTSAYFWPG
Sbjct: 481  NQAAAKAVMFISGVAVGNECYDCVSMNDTPINCSGNMRHLIVEACISRNLLDTSAYFWPG 540

Query: 563  YVNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSV 622
            YVN R SQVP S S Q+VGWSSFMKGSSLTPSM+NALVATPASSLAEIEKIYEIA NGS 
Sbjct: 541  YVNTRSSQVPRSASSQIVGWSSFMKGSSLTPSMVNALVATPASSLAEIEKIYEIAINGSG 600

Query: 623  DEKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLV 682
            DEKISAAS+LCGASLVRGWN+QEH VLFI+RLLSPPIPADY G+DSYLIDYA FLN LLV
Sbjct: 601  DEKISAASILCGASLVRGWNLQEHTVLFISRLLSPPIPADYPGSDSYLIDYAPFLNILLV 660

Query: 683  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTCRAVFSLAFTLL 742
            GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSS PKSWIL SGEELTC AVFSLAFTLL
Sbjct: 661  GISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSTPKSWILASGEELTCHAVFSLAFTLL 720

Query: 743  LRLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLKF 802
            LRLWRFHHPP+ENVKGDARPVGSQLTPEYLLLVRN+QLASFG+SPKDRL+ +RLSKLLKF
Sbjct: 721  LRLWRFHHPPIENVKGDARPVGSQLTPEYLLLVRNSQLASFGKSPKDRLKVRRLSKLLKF 780

Query: 803  SVKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLA 862
            S++P +MD+FPKLKGWYRQHQECIASI PGLVPGAPVHQ VDAL+ MMFKKINRGGQSL 
Sbjct: 781  SLEPTFMDSFPKLKGWYRQHQECIASIPPGLVPGAPVHQTVDALLTMMFKKINRGGQSLT 840

Query: 863  STTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDL 922
            STTSGSSN+SGSA+EEASIKLKVP+WDIL+ATPFVLDAALTACAHGRLS RDLATGLKDL
Sbjct: 841  STTSGSSNSSGSANEEASIKLKVPSWDILEATPFVLDAALTACAHGRLSPRDLATGLKDL 900

Query: 923  ADFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVP 982
            ADFLPA+FATIV YFSAEVTRGIWKPAFMNGTDWPSPAATLS+VEQQIK ILAATGVDVP
Sbjct: 901  ADFLPASFATIVCYFSAEVTRGIWKPAFMNGTDWPSPAATLSVVEQQIKKILAATGVDVP 960

Query: 983  SLAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIAS 1042
            SLA+GG+ PA LPLPLAALISLTITYKLDKASERLLALVGPALNS+ A CSWPCTPIIAS
Sbjct: 961  SLALGGSFPAMLPLPLAALISLTITYKLDKASERLLALVGPALNSLVAGCSWPCTPIIAS 1020

Query: 1043 LWSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGH 1102
            LW+QKVKRWNDFLVFSASRTVFHHN DAVVQLLKSCFTSTLGLGNSNV S GGVG+LLGH
Sbjct: 1021 LWAQKVKRWNDFLVFSASRTVFHHNSDAVVQLLKSCFTSTLGLGNSNVNSGGGVGALLGH 1080

Query: 1103 GFGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKL 1162
            GFGS+VLGGMSPVAPGILYLRVHR +RD+LF+VEEIVS+LMLSV+DIAV+G+PKEKAEKL
Sbjct: 1081 GFGSHVLGGMSPVAPGILYLRVHRCVRDALFLVEEIVSLLMLSVKDIAVTGVPKEKAEKL 1140

Query: 1163 KKAKYGMRYEQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVE 1222
            KK+K+GMR EQVSFASAMARVKLAASLGASLVWI GGSGLVQSL+KETLPSWFLSVHSV+
Sbjct: 1141 KKSKHGMRCEQVSFASAMARVKLAASLGASLVWISGGSGLVQSLYKETLPSWFLSVHSVD 1200

Query: 1223 REGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLASALDGK 1282
            REGVE GGMV VL GYALAFFSVLCG FSWGIDS SSASKRRAKILDSHLEFLASALDGK
Sbjct: 1201 REGVEYGGMVPVLRGYALAFFSVLCGMFSWGIDSTSSASKRRAKILDSHLEFLASALDGK 1260

Query: 1283 FSIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESG 1342
            FSIGCDW TWRAYVSGFVSL+VRCAP+WLLEVD+ +LKRL KGLRQLNEEELALALLESG
Sbjct: 1261 FSIGCDWATWRAYVSGFVSLLVRCAPKWLLEVDLKVLKRLGKGLRQLNEEELALALLESG 1320

Query: 1343 GVNAMGAAAELIIEGGF 1359
            G+ AMGAAAELIIEGGF
Sbjct: 1321 GLTAMGAAAELIIEGGF 1336

BLAST of Sed0026391 vs. ExPASy TrEMBL
Match: A0A1S3BMJ1 (mediator of RNA polymerase II transcription subunit 33B-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491278 PE=4 SV=1)

HSP 1 Score: 2312.0 bits (5990), Expect = 0.0e+00
Identity = 1183/1336 (88.55%), Postives = 1254/1336 (93.86%), Query Frame = 0

Query: 23   MAVSDPPPGQLPVVSGLWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAH 82
            MAVS  PPGQL  ++GLWD VLE+TKSAQ KN DPLLWA+QLSSTL+SA VSLPS+ELA 
Sbjct: 1    MAVSAQPPGQLQGIAGLWDTVLEVTKSAQDKNCDPLLWAVQLSSTLNSAGVSLPSVELAQ 60

Query: 83   VLVSHICWDNHVPITWKFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLN 142
            +LVSHICWDNHVPI WKFL++AMTA IVPPLLVIALLSTRAIPYRKL+ AAYRLYLELL+
Sbjct: 61   LLVSHICWDNHVPIMWKFLEKAMTARIVPPLLVIALLSTRAIPYRKLQPAAYRLYLELLS 120

Query: 143  RHIFSLTLQINGPNYQRIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLD 202
            RH+FS T QI GPNYQRIMQTIDDVLHL+QIFGLQTCEPG+LMVELFFSIVWQLLDASLD
Sbjct: 121  RHVFSSTSQIYGPNYQRIMQTIDDVLHLTQIFGLQTCEPGVLMVELFFSIVWQLLDASLD 180

Query: 203  DEELLVLPAEERSAWLIRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQ 262
            DE LL LP EE+SAWLIRPQ HDM+LDVH+S  EK+TEN E LLK+N  KAIE+IGQFLQ
Sbjct: 181  DEGLLALPGEEKSAWLIRPQLHDMELDVHDSFGEKKTENSESLLKVNTAKAIEIIGQFLQ 240

Query: 263  NKKTARILCLAQQNMPLHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSR 322
            NKKT RILCLA +NMPL WAGFA+RLQLL ANSVVL N KLIT EVLLHWTSDK++LLS+
Sbjct: 241  NKKTERILCLALRNMPLQWAGFAQRLQLLGANSVVLGNAKLITPEVLLHWTSDKNKLLSQ 300

Query: 323  EGETTSQLVFRDVIMASGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVER 382
            +G+ TSQL FRDV M+SGSLFSSAGQSHGVN SALWLPIDLFLEDAMDGSQVLATSAVER
Sbjct: 301  KGK-TSQLEFRDV-MSSGSLFSSAGQSHGVNWSALWLPIDLFLEDAMDGSQVLATSAVER 360

Query: 383  LICLTKSLRAVNDTSWHHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAV 442
            LICL KSLRAVNDTSWH+TFLGLWIAALRLIQRER+PSEGPVPRLDTCLCMLLSI+TLAV
Sbjct: 361  LICLIKSLRAVNDTSWHNTFLGLWIAALRLIQRERDPSEGPVPRLDTCLCMLLSISTLAV 420

Query: 443  TIIIEEEECELKEEECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVAN 502
            TIIIEEEE E KE++CSPSKSRDE+QSSG CRKGLITSLQMLGEYESLL PPQS+IAVAN
Sbjct: 421  TIIIEEEEVEPKEDDCSPSKSRDEKQSSGMCRKGLITSLQMLGEYESLLTPPQSIIAVAN 480

Query: 503  QAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPGY 562
            QAAAKAVMFI+GVAVGNEYYDC SMND PI CSGNMRHLIVEACISRNLLDTSAYFWPGY
Sbjct: 481  QAAAKAVMFISGVAVGNEYYDCASMNDAPINCSGNMRHLIVEACISRNLLDTSAYFWPGY 540

Query: 563  VNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSVD 622
            VNA  SQVP S S QVVGWSSFMKGS LTPSM+NALVATPASSLAEIEKIYEIA NGS D
Sbjct: 541  VNALSSQVPRSASNQVVGWSSFMKGSPLTPSMVNALVATPASSLAEIEKIYEIAINGSGD 600

Query: 623  EKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLVG 682
            EKISAAS+LCGASLVRGW +QEH  LFI+RLL PPIP DYSG+DSYLIDYA FLN LLVG
Sbjct: 601  EKISAASILCGASLVRGWYLQEHTALFISRLLLPPIPTDYSGSDSYLIDYAPFLNVLLVG 660

Query: 683  ISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTCRAVFSLAFTLLL 742
            ISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTC AVFSLAFTLLL
Sbjct: 661  ISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPKSWILTSGEELTCHAVFSLAFTLLL 720

Query: 743  RLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLKFS 802
            RLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRN+QLASFG+SP DRL+A+RLSKLLKFS
Sbjct: 721  RLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNSQLASFGKSPNDRLKARRLSKLLKFS 780

Query: 803  VKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLAS 862
            ++P++MD+FPKLKGWYRQHQECIASIL GLVPGAPVHQIVDAL+ MMF+KINRGGQSL S
Sbjct: 781  LQPIFMDSFPKLKGWYRQHQECIASILSGLVPGAPVHQIVDALLTMMFRKINRGGQSLTS 840

Query: 863  TTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDLA 922
            TTSGSSN+SGSA+EEASIKLKVPAWDIL+ATPFVLDAALTACAHGRLS RDLATGLKDLA
Sbjct: 841  TTSGSSNSSGSANEEASIKLKVPAWDILEATPFVLDAALTACAHGRLSPRDLATGLKDLA 900

Query: 923  DFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVPS 982
            DFLPA+FATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIK ILAATGVDVP 
Sbjct: 901  DFLPASFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKKILAATGVDVPC 960

Query: 983  LAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIASL 1042
            LAVGG+SPA LPLPLAALISLTITYKLDKASERLLALVGPALNS+AASCSWPCTPIIASL
Sbjct: 961  LAVGGSSPAMLPLPLAALISLTITYKLDKASERLLALVGPALNSLAASCSWPCTPIIASL 1020

Query: 1043 WSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGHG 1102
            W+QKVKRWNDFLVFSASRTVFHHN DAVVQLLKSCFTSTLGLGNSN  +SGGVG+LLGHG
Sbjct: 1021 WAQKVKRWNDFLVFSASRTVFHHNSDAVVQLLKSCFTSTLGLGNSNGNNSGGVGTLLGHG 1080

Query: 1103 FGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKLK 1162
            FGS+VLGGMSPVAPGILYLRVHRS+RD LF+VEEIVS+LMLSVRDIAVSGLPKEKAEKLK
Sbjct: 1081 FGSHVLGGMSPVAPGILYLRVHRSVRDVLFVVEEIVSLLMLSVRDIAVSGLPKEKAEKLK 1140

Query: 1163 KAKYGMRYEQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVER 1222
            K KYGMRYEQVSFASAMARVKLAASLGASLVWI GGSGLVQSLFKETLPSWFLSVHSVER
Sbjct: 1141 KTKYGMRYEQVSFASAMARVKLAASLGASLVWISGGSGLVQSLFKETLPSWFLSVHSVER 1200

Query: 1223 EGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLASALDGKF 1282
            EGV  GGMVAVL G+ALAFFSVLCGTFSWGIDS+SSASKRRAKILDS+LEFLASALDGKF
Sbjct: 1201 EGVNYGGMVAVLRGHALAFFSVLCGTFSWGIDSSSSASKRRAKILDSYLEFLASALDGKF 1260

Query: 1283 SIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESGG 1342
            SIGCDW TWRAYVSGFVSL+VRCAPRWLLEVD+N+L RLS GLRQLNEEEL L LLESGG
Sbjct: 1261 SIGCDWATWRAYVSGFVSLIVRCAPRWLLEVDLNVLTRLSNGLRQLNEEELGLELLESGG 1320

Query: 1343 VNAMGAAAELIIEGGF 1359
            VNAMGAAAELIIEGGF
Sbjct: 1321 VNAMGAAAELIIEGGF 1334

BLAST of Sed0026391 vs. ExPASy TrEMBL
Match: A0A1S4DXP9 (mediator of RNA polymerase II transcription subunit 33A-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491278 PE=4 SV=1)

HSP 1 Score: 2094.7 bits (5426), Expect = 0.0e+00
Identity = 1072/1198 (89.48%), Postives = 1131/1198 (94.41%), Query Frame = 0

Query: 161  MQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLDDEELLVLPAEERSAWLIR 220
            MQTIDDVLHL+QIFGLQTCEPG+LMVELFFSIVWQLLDASLDDE LL LP EE+SAWLIR
Sbjct: 1    MQTIDDVLHLTQIFGLQTCEPGVLMVELFFSIVWQLLDASLDDEGLLALPGEEKSAWLIR 60

Query: 221  PQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQNKKTARILCLAQQNMPLH 280
            PQ HDM+LDVH+S  EK+TEN E LLK+N  KAIE+IGQFLQNKKT RILCLA +NMPL 
Sbjct: 61   PQLHDMELDVHDSFGEKKTENSESLLKVNTAKAIEIIGQFLQNKKTERILCLALRNMPLQ 120

Query: 281  WAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSREGETTSQLVFRDVIMASG 340
            WAGFA+RLQLL ANSVVL N KLIT EVLLHWTSDK++LLS++G+ TSQL FRDV M+SG
Sbjct: 121  WAGFAQRLQLLGANSVVLGNAKLITPEVLLHWTSDKNKLLSQKGK-TSQLEFRDV-MSSG 180

Query: 341  SLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVERLICLTKSLRAVNDTSWHH 400
            SLFSSAGQSHGVN SALWLPIDLFLEDAMDGSQVLATSAVERLICL KSLRAVNDTSWH+
Sbjct: 181  SLFSSAGQSHGVNWSALWLPIDLFLEDAMDGSQVLATSAVERLICLIKSLRAVNDTSWHN 240

Query: 401  TFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAVTIIIEEEECELKEEECSP 460
            TFLGLWIAALRLIQRER+PSEGPVPRLDTCLCMLLSI+TLAVTIIIEEEE E KE++CSP
Sbjct: 241  TFLGLWIAALRLIQRERDPSEGPVPRLDTCLCMLLSISTLAVTIIIEEEEVEPKEDDCSP 300

Query: 461  SKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVANQAAAKAVMFIAGVAVGNE 520
            SKSRDE+QSSG CRKGLITSLQMLGEYESLL PPQS+IAVANQAAAKAVMFI+GVAVGNE
Sbjct: 301  SKSRDEKQSSGMCRKGLITSLQMLGEYESLLTPPQSIIAVANQAAAKAVMFISGVAVGNE 360

Query: 521  YYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPGYVNARKSQVPSSTSGQVVG 580
            YYDC SMND PI CSGNMRHLIVEACISRNLLDTSAYFWPGYVNA  SQVP S S QVVG
Sbjct: 361  YYDCASMNDAPINCSGNMRHLIVEACISRNLLDTSAYFWPGYVNALSSQVPRSASNQVVG 420

Query: 581  WSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSVDEKISAASVLCGASLVRGW 640
            WSSFMKGS LTPSM+NALVATPASSLAEIEKIYEIA NGS DEKISAAS+LCGASLVRGW
Sbjct: 421  WSSFMKGSPLTPSMVNALVATPASSLAEIEKIYEIAINGSGDEKISAASILCGASLVRGW 480

Query: 641  NIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLVGISSVDCVQIFSLHGMVPL 700
             +QEH  LFI+RLL PPIP DYSG+DSYLIDYA FLN LLVGISSVDCVQIFSLHGMVPL
Sbjct: 481  YLQEHTALFISRLLLPPIPTDYSGSDSYLIDYAPFLNVLLVGISSVDCVQIFSLHGMVPL 540

Query: 701  LAGQLMPICEAFGSSPPKSWILTSGEELTCRAVFSLAFTLLLRLWRFHHPPVENVKGDAR 760
            LAGQLMPICEAFGSSPPKSWILTSGEELTC AVFSLAFTLLLRLWRFHHPPVENVKGDAR
Sbjct: 541  LAGQLMPICEAFGSSPPKSWILTSGEELTCHAVFSLAFTLLLRLWRFHHPPVENVKGDAR 600

Query: 761  PVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLKFSVKPVYMDAFPKLKGWYRQ 820
            PVGSQLTPEYLLLVRN+QLASFG+SP DRL+A+RLSKLLKFS++P++MD+FPKLKGWYRQ
Sbjct: 601  PVGSQLTPEYLLLVRNSQLASFGKSPNDRLKARRLSKLLKFSLQPIFMDSFPKLKGWYRQ 660

Query: 821  HQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLASTTSGSSNTSGSASEEASI 880
            HQECIASIL GLVPGAPVHQIVDAL+ MMF+KINRGGQSL STTSGSSN+SGSA+EEASI
Sbjct: 661  HQECIASILSGLVPGAPVHQIVDALLTMMFRKINRGGQSLTSTTSGSSNSSGSANEEASI 720

Query: 881  KLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDLADFLPATFATIVSYFSAEV 940
            KLKVPAWDIL+ATPFVLDAALTACAHGRLS RDLATGLKDLADFLPA+FATIVSYFSAEV
Sbjct: 721  KLKVPAWDILEATPFVLDAALTACAHGRLSPRDLATGLKDLADFLPASFATIVSYFSAEV 780

Query: 941  TRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVPSLAVGGTSPATLPLPLAAL 1000
            TRGIWKPAFMNGTDWPSPAATLSIVEQQIK ILAATGVDVP LAVGG+SPA LPLPLAAL
Sbjct: 781  TRGIWKPAFMNGTDWPSPAATLSIVEQQIKKILAATGVDVPCLAVGGSSPAMLPLPLAAL 840

Query: 1001 ISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIASLWSQKVKRWNDFLVFSASR 1060
            ISLTITYKLDKASERLLALVGPALNS+AASCSWPCTPIIASLW+QKVKRWNDFLVFSASR
Sbjct: 841  ISLTITYKLDKASERLLALVGPALNSLAASCSWPCTPIIASLWAQKVKRWNDFLVFSASR 900

Query: 1061 TVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGHGFGSNVLGGMSPVAPGILY 1120
            TVFHHN DAVVQLLKSCFTSTLGLGNSN  +SGGVG+LLGHGFGS+VLGGMSPVAPGILY
Sbjct: 901  TVFHHNSDAVVQLLKSCFTSTLGLGNSNGNNSGGVGTLLGHGFGSHVLGGMSPVAPGILY 960

Query: 1121 LRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKLKKAKYGMRYEQVSFASAMA 1180
            LRVHRS+RD LF+VEEIVS+LMLSVRDIAVSGLPKEKAEKLKK KYGMRYEQVSFASAMA
Sbjct: 961  LRVHRSVRDVLFVVEEIVSLLMLSVRDIAVSGLPKEKAEKLKKTKYGMRYEQVSFASAMA 1020

Query: 1181 RVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVEREGVECGGMVAVLGGYALA 1240
            RVKLAASLGASLVWI GGSGLVQSLFKETLPSWFLSVHSVEREGV  GGMVAVL G+ALA
Sbjct: 1021 RVKLAASLGASLVWISGGSGLVQSLFKETLPSWFLSVHSVEREGVNYGGMVAVLRGHALA 1080

Query: 1241 FFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLASALDGKFSIGCDWGTWRAYVSGFVS 1300
            FFSVLCGTFSWGIDS+SSASKRRAKILDS+LEFLASALDGKFSIGCDW TWRAYVSGFVS
Sbjct: 1081 FFSVLCGTFSWGIDSSSSASKRRAKILDSYLEFLASALDGKFSIGCDWATWRAYVSGFVS 1140

Query: 1301 LMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESGGVNAMGAAAELIIEGGF 1359
            L+VRCAPRWLLEVD+N+L RLS GLRQLNEEEL L LLESGGVNAMGAAAELIIEGGF
Sbjct: 1141 LIVRCAPRWLLEVDLNVLTRLSNGLRQLNEEELGLELLESGGVNAMGAAAELIIEGGF 1196

BLAST of Sed0026391 vs. TAIR 10
Match: AT3G23590.1 (REF4-related 1 )

HSP 1 Score: 1457.2 bits (3771), Expect = 0.0e+00
Identity = 767/1319 (58.15%), Postives = 973/1319 (73.77%), Query Frame = 0

Query: 39   LWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAHVLVSHICWDNHVPITW 98
            +WD V+ELTK AQ    DP LWA QLSS L    V LPS ELA V+VS+ICWDN+VPI W
Sbjct: 9    VWDCVIELTKMAQENCVDPRLWASQLSSNLKFFAVELPSTELAEVIVSYICWDNNVPIVW 68

Query: 99   KFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLNRHIFSLTLQINGPNYQ 158
            KFL++AM   +V PL+V+ALL+ R +P R  + AAYR+YLELL R++F++   I+GP+YQ
Sbjct: 69   KFLERAMALKLVSPLVVLALLADRVVPTRSTQQAAYRIYLELLKRNMFTIKDHISGPHYQ 128

Query: 159  RIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLDDEELLVLPAEERSAWL 218
            ++M ++ ++L LS++F L T +PG+L+VE  F +V QLLDA+L DE LL L  +  S WL
Sbjct: 129  KVMISVSNILRLSELFDLDTSKPGVLLVEFVFKMVSQLLDAALSDEGLLELSQDSSSQWL 188

Query: 219  IRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQNKKTARILCLAQQNMP 278
            ++ Q  DM++D  E  +EK T + E+L  +N   AIELI +FL+N   AR+L L   N  
Sbjct: 189  VKSQ--DMEIDAPERYNEK-TGSLEKLQSLNTIMAIELIAEFLRNTVIARLLYLVSSNRA 248

Query: 279  LHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSREGETTSQLVFRDVIMA 338
              W  F +++QLL  NS  LK++K++ S  LL   S++    S + + TS     + I+ 
Sbjct: 249  SKWHEFVQKVQLLGENSSALKHSKVLNSGDLLQLISNRRFGYSYDSKVTSARK-SNAIVD 308

Query: 339  SGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVERLICLTKSLRAVNDTSW 398
             GSL S AG  HG +LS+LWLP+DL  EDAMDG QV  TSA+E +  L K+L+ +N ++W
Sbjct: 309  FGSLSSYAGLCHGASLSSLWLPLDLVFEDAMDGYQVNPTSAIEIITGLAKTLKEINGSTW 368

Query: 399  HHTFLGLWIAALRLIQRERNPSEGPVPRLDTCLCMLLSITTLAVTIIIEEEECELKEEEC 458
            H TFLGLWIAALRL+QRER+P EGP+PRLDT LCM L I  L V  +IEE + E   E  
Sbjct: 369  HDTFLGLWIAALRLVQRERDPIEGPIPRLDTRLCMSLCIVPLVVANLIEEGKYESVME-- 428

Query: 459  SPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIAVANQAAAKAVMFIAGVAVG 518
                         K R  L+TSLQ+LG++  LLAPP+ V++ AN+AA KA++F++G  VG
Sbjct: 429  -------------KLRDDLVTSLQVLGDFPGLLAPPKCVVSAANKAATKAILFLSGGNVG 488

Query: 519  NEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFWPGYVNARKSQVPSSTSGQV 578
               +D ++M D P+ CSGNMRHLIVEACI+RN+LD SAY WPGYVN R +Q+P S   +V
Sbjct: 489  KSCFDVINMKDMPVNCSGNMRHLIVEACIARNILDMSAYSWPGYVNGRINQIPQSLPNEV 548

Query: 579  VGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENGSVDEKISAASVLCGASLVR 638
              WSSF+KG+ L  +M+N LV+ PASSLAE+EK++E+A  GS DEKISAA+VLCGASL R
Sbjct: 549  PCWSSFVKGAPLNAAMVNTLVSVPASSLAELEKLFEVAVKGSDDEKISAATVLCGASLTR 608

Query: 639  GWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNALLVGISSVDCVQIFSLHGMV 698
            GWNIQEH V ++ RLLSPP+PADYS  +++LI YA  LN ++VGI SVD +QIFSLHGMV
Sbjct: 609  GWNIQEHTVEYLTRLLSPPVPADYSRAENHLIGYACMLNVVIVGIGSVDSIQIFSLHGMV 668

Query: 699  PLLAGQLMPICEAFGS-SPPKSWILTSGEELTCRAVFSLAFTLLLRLWRFHHPPVENVKG 758
            P LA  LMPICE FGS +P  SW L SGE ++  +VFS AFTLLL+LWRF+HPP+E+  G
Sbjct: 669  PQLACSLMPICEEFGSYTPSVSWTLPSGEAISAYSVFSNAFTLLLKLWRFNHPPIEHGVG 728

Query: 759  DARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKLLK-FSVKPVYMDAFPKLKG 818
            D   VGSQLTPE+LL VRN+ L S     +DR R KRLS++ +  S +PV++D+FPKLK 
Sbjct: 729  DVPTVGSQLTPEHLLSVRNSYLVSSEILDRDRNR-KRLSEVARAASCQPVFVDSFPKLKV 788

Query: 819  WYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQSLASTTSGSSNTSGSASE 878
            WYRQHQ CIA+ L GL  G+PVHQ V+AL+ M F K+ RG Q+L    SG+S++SG+ASE
Sbjct: 789  WYRQHQRCIAATLSGLTHGSPVHQTVEALLNMTFGKV-RGSQTLNPVNSGTSSSSGAASE 848

Query: 879  EASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGLKDLADFLPATFATIVSYF 938
            +++I+ + PAWDILKA P+V+DAALTAC HGRLS R LATGLKDLADFLPA+ ATIVSYF
Sbjct: 849  DSNIRPEFPAWDILKAVPYVVDAALTACTHGRLSPRQLATGLKDLADFLPASLATIVSYF 908

Query: 939  SAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGVDVPSLAVGGTSPATLPLP 998
            SAEV+RG+WKP FMNG DWPSPA  LS VE+ I  ILA TGVD+PSLA GG+SPATLPLP
Sbjct: 909  SAEVSRGVWKPVFMNGVDWPSPATNLSTVEEYITKILATTGVDIPSLAPGGSSPATLPLP 968

Query: 999  LAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPIIASLWSQKVKRWNDFLVF 1058
            LAA +SLTITYK+DKASER L L GPAL  +AA C WPC PI+ASLW+QK KRW DFLVF
Sbjct: 969  LAAFVSLTITYKIDKASERFLNLAGPALECLAAGCPWPCMPIVASLWTQKAKRWFDFLVF 1028

Query: 1059 SASRTVFHHNMDAVVQLLKSCFTSTLGLGNSNVFSSGGVGSLLGHGFGSNVLGGMSPVAP 1118
            SASRTVF HN DAV+QLL++CF++TLGL  + + + GGVG+LLGHGFGS+  GG+SPVAP
Sbjct: 1029 SASRTVFLHNQDAVIQLLRNCFSATLGLNAAPMSNDGGVGALLGHGFGSHFYGGISPVAP 1088

Query: 1119 GILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEKAEKLKKAKYGMRYEQVSFA 1178
            GILYLR++R++RD++ + EEI+S+L+ SV DIA + L KEK EKLK  K G RY Q S A
Sbjct: 1089 GILYLRMYRALRDTVSVSEEILSLLIHSVEDIAQNRLSKEKLEKLKTVKNGSRYGQSSLA 1148

Query: 1179 SAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFLSVHSVEREGVECGGMVAVLGG 1238
            +AM +VKLAASL ASLVW+ GG G+V  L KET+PSWFLS    +RE      +VA L G
Sbjct: 1149 TAMTQVKLAASLSASLVWLTGGLGVVHVLIKETIPSWFLSTDKSDREQGP-SDLVAELRG 1208

Query: 1239 YALAFFSVLCGTFSWGIDSASSASKRRAK-ILDSHLEFLASALDGKFSIGCDWGTWRAYV 1298
            +ALA+F VLCG  +WG+DS SSASKRR + IL SHLEF+ASALDGK S+GC+  TWR Y+
Sbjct: 1209 HALAYFVVLCGALTWGVDSRSSASKRRRQAILGSHLEFIASALDGKISVGCETATWRTYI 1268

Query: 1299 SGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELALALLESGGVNAMGAAAELII 1355
            SG VSLMV C P W+ E+D  +LK LS GLR+  ++ELA+ LL  GG+  M  AA+ II
Sbjct: 1269 SGLVSLMVSCLPLWVTEIDTEVLKSLSNGLRKWGKDELAIVLLSLGGLKTMDYAADFII 1305

BLAST of Sed0026391 vs. TAIR 10
Match: AT2G48110.1 (reduced epidermal fluorescence 4 )

HSP 1 Score: 1446.4 bits (3743), Expect = 0.0e+00
Identity = 790/1340 (58.96%), Postives = 953/1340 (71.12%), Query Frame = 0

Query: 39   LWDRVLELTKSAQAKNRDPLLWALQLSSTLDSARVSLPSLELAHVLVSHICWDNHVPITW 98
            LW+ V  L +SAQ KN DPL WALQL  TL SA +SLPS +LA  LV+HI W+NH P++W
Sbjct: 10   LWESVTSLIRSAQEKNVDPLHWALQLRLTLASAGISLPSPDLAQFLVTHIFWENHSPLSW 69

Query: 99   KFLDQAMTANIVPPLLVIALLSTRAIPYRKLRTAAYRLYLELLNRHIFSLTLQINGPNYQ 158
            K L++A++ NIVPPLLV+ALLS R IP RKL  AAYRLY+ELL RH FS    I  P Y 
Sbjct: 70   KLLEKAISVNIVPPLLVLALLSPRVIPNRKLHPAAYRLYMELLKRHAFSFMPLIRAPGYH 129

Query: 159  RIMQTIDDVLHLSQIFGLQTCEPGLLMVELFFSIVWQLLDASLDDEELLVLPAEERSAWL 218
            + M +IDD+LHLS+ FG+Q  EPG +++   FSIVW+LLDASLD+E LL L + +RS W 
Sbjct: 130  KTMNSIDDILHLSETFGVQDQEPGSILLAFVFSIVWELLDASLDEEGLLELTSNKRSKW- 189

Query: 219  IRPQPHDMKLDVHESLSEKRTENGERLLKINNEKAIELIGQFLQNKKTARILCLAQQNMP 278
                PHDM LD  E+ S KR EN + L K N E AIELI +FLQNK T+RIL LA QNM 
Sbjct: 190  -PSSPHDMDLDGLEN-SVKRNENHDALEKANTEMAIELIQEFLQNKVTSRILHLASQNM- 249

Query: 279  LHWAGFARRLQLLAANSVVLKNTKLITSEVLLHWTSDKHRLLSREGETTSQLVFRDVIMA 338
                                                        E +T  +  F   I++
Sbjct: 250  --------------------------------------------ESKTIPRGEFH-AIVS 309

Query: 339  SGSLFSSAGQSHGVNLSALWLPIDLFLEDAMDGSQVLATSAVERLICLTKSLRAVNDTSW 398
            SGS  +          SALWLPIDLF ED MDG+Q  A SAVE L  L K+L+A N TSW
Sbjct: 310  SGSKLALTSD------SALWLPIDLFFEDIMDGTQAAAASAVENLTGLVKALQAANSTSW 369

Query: 399  HHTFLGLWIAALRLIQR-------------------ERNPSEGPVPRLDTCLCMLLSITT 458
            H  FL LW+AALRL+QR                   ER+P EGPVPR DT LC+LLS+T 
Sbjct: 370  HDAFLALWLAALRLVQRENLCLRYCFFMHMLEILSEERDPIEGPVPRTDTFLCVLLSVTP 429

Query: 459  LAVTIIIEEEECELKEEECSPSKSRDERQSSGKCRKGLITSLQMLGEYESLLAPPQSVIA 518
            LAV  IIEEEE +   ++ S S S   ++  GKCR+GLI SLQ LG+YESLL PP+SV +
Sbjct: 430  LAVANIIEEEESQW-IDQTSSSPSNQWKEKKGKCRQGLINSLQQLGDYESLLTPPRSVQS 489

Query: 519  VANQAAAKAVMFIAGVAVGNEYYDCVSMNDTPIKCSGNMRHLIVEACISRNLLDTSAYFW 578
            VANQAAAKA+MFI+G+   N  Y+  SM+++   C           C  R  L T   F 
Sbjct: 490  VANQAAAKAIMFISGITNSNGSYENTSMSESASGC-----------CKVRFSLFTLKMFV 549

Query: 579  PGYVNARKSQVPSSTSGQVVGWSSFMKGSSLTPSMINALVATPASSLAEIEKIYEIAENG 638
               V    +         +  WS  MKGS LTPS+ N+L+ TPASSLAEIEK+YE+A  G
Sbjct: 550  VMGVYLLCN---------ISCWSLVMKGSPLTPSLTNSLITTPASSLAEIEKMYEVATTG 609

Query: 639  SVDEKISAASVLCGASLVRGWNIQEHIVLFIARLLSPPIPADYSGNDSYLIDYASFLNAL 698
            S DEKI+ AS+LCGASL RGW+IQEH+++FI  LLSPP PAD SG+ S+LI+ A FLN L
Sbjct: 610  SEDEKIAVASILCGASLFRGWSIQEHVIIFIVTLLSPPAPADLSGSYSHLINSAPFLNVL 669

Query: 699  LVGISSVDCVQIFSLHGMVPLLAGQLMPICEAFGSSPPK-SWILTSGEELTCRAVFSLAF 758
            LVGIS +DCV IFSLHG+VPLLAG LMPICEAFGS  P  +W L +GE ++  AVFS AF
Sbjct: 670  LVGISPIDCVHIFSLHGVVPLLAGALMPICEAFGSGVPNITWTLPTGELISSHAVFSTAF 729

Query: 759  TLLLRLWRFHHPPVENVKGDARPVGSQLTPEYLLLVRNTQLASFGQSPKDRLRAKRLSKL 818
            TLLLRLWRF HPP++ V GD  PVG Q +PEYLLLVRN +L  FG+SPKDR+  +R SK+
Sbjct: 730  TLLLRLWRFDHPPLDYVLGDVPPVGPQPSPEYLLLVRNCRLECFGKSPKDRMARRRFSKV 789

Query: 819  LKFSVKPVYMDAFPKLKGWYRQHQECIASILPGLVPGAPVHQIVDALMIMMFKKINRGGQ 878
            +  SV P++MD+FP+LK WYRQHQEC+ASIL  L  G+PVH IVD+L+ MMFKK N+GG 
Sbjct: 790  IDISVDPIFMDSFPRLKQWYRQHQECMASILSELKTGSPVHHIVDSLLSMMFKKANKGGS 849

Query: 879  SLASTTSGSSNTSGSASEEASIKLKVPAWDILKATPFVLDAALTACAHGRLSTRDLATGL 938
               + +SGSS+ S S  +++S +LK+PAWDIL+A PFVLDAALTACAHG LS R+LATGL
Sbjct: 850  QSLTPSSGSSSLSTSGGDDSSDQLKLPAWDILEAAPFVLDAALTACAHGSLSPRELATGL 909

Query: 939  KDLADFLPATFATIVSYFSAEVTRGIWKPAFMNGTDWPSPAATLSIVEQQIKSILAATGV 998
            K LADFLPAT  T+VSYFS+EVTRG+WKP  MNGTDWPSPAA L+ VEQQI+ ILAATGV
Sbjct: 910  KILADFLPATLGTMVSYFSSEVTRGLWKPVSMNGTDWPSPAANLASVEQQIEKILAATGV 969

Query: 999  DVPSLAVGGTSPATLPLPLAALISLTITYKLDKASERLLALVGPALNSIAASCSWPCTPI 1058
            DVP L   G S ATLPLPLAAL+SLTITYKLDKA+ER L LVGPAL+S+AA+C WPC PI
Sbjct: 970  DVPRLPADGISAATLPLPLAALVSLTITYKLDKATERFLVLVGPALDSLAAACPWPCMPI 1029

Query: 1059 IASLWSQKVKRWNDFLVFSASRTVFHHNMDAVVQLLKSCFTSTLGL-GNSNVFSSGGVGS 1118
            + SLW+QKVKRW+DFL+FSASRTVFHHN DAV+QLL+SCFT TLGL   S + S GGVG+
Sbjct: 1030 VTSLWTQKVKRWSDFLIFSASRTVFHHNRDAVIQLLRSCFTCTLGLTPTSQLCSYGGVGA 1089

Query: 1119 LLGHGFGSNVLGGMSPVAPGILYLRVHRSMRDSLFMVEEIVSILMLSVRDIAVSGLPKEK 1178
            LLGHGFGS   GG+S  APGILY++VHRS+RD +F+ EEI+S+LM SV+ IA   LP  +
Sbjct: 1090 LLGHGFGSRYSGGISTAAPGILYIKVHRSIRDVMFLTEEILSLLMFSVKSIATRELPAGQ 1149

Query: 1179 AEKLKKAKYGMRY--EQVSFASAMARVKLAASLGASLVWIFGGSGLVQSLFKETLPSWFL 1238
            AEKLKK K G RY   QVS + AM RVKLAASLGASLVWI GG  LVQ+L KETLPSWF+
Sbjct: 1150 AEKLKKTKDGSRYGIGQVSLSLAMRRVKLAASLGASLVWISGGLNLVQALIKETLPSWFI 1209

Query: 1239 SVHSVEREGVECGGMVAVLGGYALAFFSVLCGTFSWGIDSASSASKRRAKILDSHLEFLA 1298
            SVH  E    E GGMV +L GYALA+F++L   F+WG+DS+  ASKRR ++L  HLEF+ 
Sbjct: 1210 SVHGEED---ELGGMVPMLRGYALAYFAILSSAFAWGVDSSYPASKRRPRVLWLHLEFMV 1269

Query: 1299 SALDGKFSIGCDWGTWRAYVSGFVSLMVRCAPRWLLEVDVNLLKRLSKGLRQLNEEELAL 1356
            SAL+GK S+GCDW TW+AYV+GFVSLMV+C P W+LEVDV ++KRLSK LRQ NE++LAL
Sbjct: 1270 SALEGKISLGCDWATWQAYVTGFVSLMVQCTPAWVLEVDVEVIKRLSKSLRQWNEQDLAL 1270