Sed0026314 (gene) Chayote v1

Overview
NameSed0026314
Typegene
OrganismSechium edule (Chayote v1)
DescriptionAspartic proteinase
LocationLG10: 19671621 .. 19675767 (-)
RNA-Seq ExpressionSed0026314
SyntenySed0026314
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAGAATCTTCAATCATCGACGCACGTCGTAGATACACGTGTATGGTAGATATTTGAAGACGAAGCTTCCTTTTTTTTTTTAATTAGAAAAAACCATAAGAGAAATTGCAGTTCATCCAGTATCTACCGTTGAACTTCTTTTCCGAAATCTCTCTTGATTCTTGTACTGAAGGTGAATACTCTCATCTCTCTGGTATTTATATACTTTTTCGATCTTCTGTTCTCTCTTTATAAATCTATTCTGGCGGCGATCGGGTTCTGTTTCGGTGCTGTTGGATTTTGTATTGAGGTTCGAACTAGGGAACTTGATTGTTTACATGTTTTTTTTGTTGGAAATAGATATTTGAAGTATTTGAATCTTAGATTCGATTACTTTAGTAGTTTTTGAGTGTTGCTAGGTCACTTTTAACATTTATGTTAAAAGAATTATGAATTGTTGCTGTAGCAATTTCTTGTTAGAAATGGGAACTTTGGTTTGCGGTTATTTATTAGTGTCCCCATGATTACTTTTGGATTTTAATTGAGCGTGTTTTCAATTCATAGTTAGCAACATGGGCACCGGACTCAAACTTTTTACAGCCGTGCTCTTCATTTGCTTCCTTACATTTCCACTGGTTTTTTCTGCATCCAATGATGGGAAGGTTAGAATTGGACTTAAAAGGAGGAATTTTGACCAGAACAACCGAGTTGCTGGCAAGATTGCAACCAAGGAATCAGTTTCCTTGAAAGGCTCTGAGAAATATCAGGCTAGTGGAAATTTAGGAGATTCGGCTGATACTGATATTGTAGCATTGAAGAATTACTTGAATGCTCAGTATTTTGGGGAGATTGGTATTGGCACTCCCCCTCAGAAGTTTACTGTTGTTTTTGACACTGGTAGTTCTAATTTATGGGTTCCATCATCTAAGTGCCTCTCTGTAAGTTCCAATCATCTTAATTGTGATAAGATTTAGTCGACTTTTTGTACAACTGCAGGCGTTGACTTTCAACTCTCTGCAGGTTGCATGTCTTCTCCATTCCAAGTACCGGTCGAGGCATTCAAGCACTTACAAGAAGAATGGTTAGTAGTACTCTTCCTACATAATGCTACGGTTTTGTAACATCATTTATATTATTTTGAGTTGCTCTTGGCTCTGAGCATTAATTTTTTGCTCTTTTTTTTGTTTTTCTACTGTAGGAAAACGTGCCTCGATTAAATATGGTACTGGTGCTATTTCTGGCTACTTTAGTCAAGACCATGTTAAAGTTGGCAACCTAGTAGTTCAGAAACAGGTATTTCTTTTTTCTTATTTATGTCCTATTCGTGTATTTATTATTGGTTATCTTATTTACTTCAATAATGTTATTCATTACAGGATTTTATCGAGGCAACTAGGGAGCCCAGCCTCACATTTGTACTGGCCCAATTTGATGGCATTTTTGGACTTGGCTTCAAAGAGATTTCAGTTGGAGATGCAGTGCCTGTATGGTATGTTTATCGTAGAAATGACTGCCCAGCTACTTGGATGTGTTATGCAAGAACTATTAAGTGTTCATGTTAGTGGTATATATTGCTAAGTGTTTAGTAGTAGTACAGGTACAACATGGTTGACCAAAATCTTGTAAAGGAACTTGTTTTCTCTTTTTGGTTTAATCGCAATGTGGATGCGGATGAGGAACAAGGTGGTGAAATTGTATTTGGTGGTGTGGACCCTTATCACTACAAGGGGGAGCATACATATGTTCCAGTTACTAAAAAAGGATATTGGCAGGTTCGTTATGTGTGCTGTTTGATAAACTGGTTTGTATGTAATTTATCATTATCTCGTTACTAGTTAGATATAAGGTATTAATTTGCATATGATTTTCTATGTTGGCAGTTTGATATGGGCGACGTTCTGATCAATGGCAGTACAACCGGTATGTTAAAATTTGCACGGCTGTGATATTCAAATATGGTTTTCATATCCTCTTTATTAACCTTTACATGAACAATCCCTATCACAGAATTTTGTTCAAATGGTTGTTCAGCCATTGCTGATTCAGGAACTTCTTTATTGGCTGGTCCAACGGTATGTTACTCTTCATGTGCAGTTGTTGAGCTAAACATATTTATTTTTTGTAGTATATAAATTTTCCTATGCTGTAGGATGCCTTTAATTTAGCTTCATTCATATTAAACCTTTCAGTTTCTCCTTCACATTGATTTCATTTCAACTTGTGGTTGTGAACGGCATGATGAATACTAAAACGTTATGGGATGAATATGGAAGATGTCTATTGTTAAATAAATATTACCTATTTATAATCATCTTGATTTGACGAGGGTCCCTTGAATGTTTGAACCTGTGTTTGATAATGTTCAAATATATTACCATTGCAAATTACAAAGATCATGGATAGTTTCATTTTCTGTTTTCGATCTTGTGTTATTTTCAACTTAGAGTTGGTTTCTTGTCTGCATTATTCTTAGCGTTTTATAAATGGCTTGAGTTTTGCCTCCCCTGATTTCAGACTATTATCACTCAAATTAATCATGCTATTGGAGCCTCCGGGGTTGTAAGTGAGGAATGCAAGACTGTTGTGGCCGAATATGGTGAAACCATAATTAAAATGCTATTAGCAAAGGTATTTTATGTCTACTTTACGTTATTATTCCTTTAACGTAAGAATCTCATTGTTTAATCCTCTTAAATTTTTTATTTTACTTTGGTAGGATCAACCAAAGAAAATCTGCTCTACACTTGGTTTGTGTACTTTTGACGGGACTCGAGGAGTGAGGTTTGAATTTTGTTTTTTCTTAGTAGAAGCTGATGTTTAGATCATCTTGTTATTACTTTTTTGTTTGGCTTCTTTTCTGGGGTGCGTAGTATTTTCATTCCTTTTCCACTTCTTGTGAAATTGGTATCAGTATGGGCATCAAGAGCGTTGTTGATAACACCACTCAGAAATCTTCGAATGGTTTGCGTGATATTCTGTGCAACACATGTGAGATGGCAGTTGTATGGGCACAAAGCCAGCTAAAAGATGAACAAACACAAGACCAGATTCTCAATTATATGGATCGGGTAAAGTAACTTATTACCCTCAGAATTTTCTTCCAAAACTCACAGCTCATGTGGTTGTCAATTGTGATTGCAGCTGTGCGAGAAGTTGCCTAGTCCAATGGGAGAATCAGTAATAGATTGCGATTCCTTGTCTACTTTGCCTAATGTTTCATTTACAATTGGTGGAAAGGTCTTTGAGCTAAAGCCTGAGCAGGTAAAATAGGCTGGTGGCTGGCTATCTGTATTGGTTTGTTGATTGCATCATGCTGCATTCTTTGGTATCTTCCATTTTTATCTTGAGCATTTCTTCTAGAGCTGCAAATTTGGGCATCTTCTGAGAAGCATGTAGTATATTTATCGTACTAGACTGAATCTGTATGTATTTCTTTTACTGCAACCAGTACGTCCTCAAAGTCACACAGGGACCTGTAACCGAATGCATCAGTGGATTTGCAGCATTGGATGTGCCGCCACCACGTGGACCTCTCTGGTTAGTCTTACTTTATCATTGACTCTATTGTATGCATCCCGAATCCAAATTTAATTAACGGGGCCTCGTTGGTGATGTAGGATATTGGGGGATGTATTCATGGGTCCATACCACACGGTGTTTGACTATGGAAACTTGAGAGTTGGGTTTGCAGAATCTGCTTAAATGCTTAAAATCTCAACTTGCTCTGTTTCTTCAAGTTGTTACCAGTTTCCACGACTCCTATGTCAATAAGCTTATAGATATGTAGCTGTGCATGTAAATCTTTATTCAGGAACCTTTGCTGTGGAAATGGACTGTATTACATTTGCGTACTTATCAGAAATACTTCTATTCTGTATTTTACAAAGACACTCTTTGGTTGTTGATCGACCATTCTCCTCGCTACGAGCAAATGGCCTCTTACACGACCACAAGGTTTGGTTCAAAATGCAGGAGTTAGATTTTGGAATGCTGATTCCTCTTATTGAGCAGCTCAGTTTGCCTCTTGGTTATGAAGTTACTAACCAAAGTTCTCTGCATTTACACATTCATTTTTAGAGATGCAAATGGATTGGGTTGGGTCCTTTTCTAAAAACTCAGCTTACCCAATTCAATTTCCTTTCCTAAGGAAGTTTTCCTTTTTCAGG

mRNA sequence

GAAAAGAATCTTCAATCATCGACGCACGTCGTAGATACACGTGTATGGTAGATATTTGAAGACGAAGCTTCCTTTTTTTTTTTAATTAGAAAAAACCATAAGAGAAATTGCAGTTCATCCAGTATCTACCGTTGAACTTCTTTTCCGAAATCTCTCTTGATTCTTGTACTGAAGTTAGCAACATGGGCACCGGACTCAAACTTTTTACAGCCGTGCTCTTCATTTGCTTCCTTACATTTCCACTGGTTTTTTCTGCATCCAATGATGGGAAGGTTAGAATTGGACTTAAAAGGAGGAATTTTGACCAGAACAACCGAGTTGCTGGCAAGATTGCAACCAAGGAATCAGTTTCCTTGAAAGGCTCTGAGAAATATCAGGCTAGTGGAAATTTAGGAGATTCGGCTGATACTGATATTGTAGCATTGAAGAATTACTTGAATGCTCAGTATTTTGGGGAGATTGGTATTGGCACTCCCCCTCAGAAGTTTACTGTTGTTTTTGACACTGGTAGTTCTAATTTATGGGTTCCATCATCTAAGTGCCTCTCTGTTGCATGTCTTCTCCATTCCAAGTACCGGTCGAGGCATTCAAGCACTTACAAGAAGAATGGAAAACGTGCCTCGATTAAATATGGTACTGGTGCTATTTCTGGCTACTTTAGTCAAGACCATGTTAAAGTTGGCAACCTAGTAGTTCAGAAACAGGATTTTATCGAGGCAACTAGGGAGCCCAGCCTCACATTTGTACTGGCCCAATTTGATGGCATTTTTGGACTTGGCTTCAAAGAGATTTCAGTTGGAGATGCAGTGCCTGTATGGTACAACATGGTTGACCAAAATCTTGTAAAGGAACTTGTTTTCTCTTTTTGGTTTAATCGCAATGTGGATGCGGATGAGGAACAAGGTGGTGAAATTGTATTTGGTGGTGTGGACCCTTATCACTACAAGGGGGAGCATACATATGTTCCAGTTACTAAAAAAGGATATTGGCAGTTTGATATGGGCGACGTTCTGATCAATGGCAGTACAACCGAATTTTGTTCAAATGGTTGTTCAGCCATTGCTGATTCAGGAACTTCTTTATTGGCTGGTCCAACGACTATTATCACTCAAATTAATCATGCTATTGGAGCCTCCGGGGTTGTAAGTGAGGAATGCAAGACTGTTGTGGCCGAATATGGTGAAACCATAATTAAAATGCTATTAGCAAAGGATCAACCAAAGAAAATCTGCTCTACACTTGGTTTGTGTACTTTTGACGGGACTCGAGGAGTGAGTATGGGCATCAAGAGCGTTGTTGATAACACCACTCAGAAATCTTCGAATGGTTTGCGTGATATTCTGTGCAACACATGTGAGATGGCAGTTGTATGGGCACAAAGCCAGCTAAAAGATGAACAAACACAAGACCAGATTCTCAATTATATGGATCGGCTGTGCGAGAAGTTGCCTAGTCCAATGGGAGAATCAGTAATAGATTGCGATTCCTTGTCTACTTTGCCTAATGTTTCATTTACAATTGGTGGAAAGGTCTTTGAGCTAAAGCCTGAGCAGTACGTCCTCAAAGTCACACAGGGACCTGTAACCGAATGCATCAGTGGATTTGCAGCATTGGATGTGCCGCCACCACGTGGACCTCTCTGGATATTGGGGGATGTATTCATGGGTCCATACCACACGGTGTTTGACTATGGAAACTTGAGAGTTGGGTTTGCAGAATCTGCTTAAATGCTTAAAATCTCAACTTGCTCTGTTTCTTCAAGTTGTTACCAGTTTCCACGACTCCTATGTCAATAAGCTTATAGATATGTAGCTGTGCATGTAAATCTTTATTCAGGAACCTTTGCTGTGGAAATGGACTGTATTACATTTGCGTACTTATCAGAAATACTTCTATTCTGTATTTTACAAAGACACTCTTTGGTTGTTGATCGACCATTCTCCTCGCTACGAGCAAATGGCCTCTTACACGACCACAAGGTTTGGTTCAAAATGCAGGAGTTAGATTTTGGAATGCTGATTCCTCTTATTGAGCAGCTCAGTTTGCCTCTTGGTTATGAAGTTACTAACCAAAGTTCTCTGCATTTACACATTCATTTTTAGAGATGCAAATGGATTGGGTTGGGTCCTTTTCTAAAAACTCAGCTTACCCAATTCAATTTCCTTTCCTAAGGAAGTTTTCCTTTTTCAGG

Coding sequence (CDS)

ATGGGCACCGGACTCAAACTTTTTACAGCCGTGCTCTTCATTTGCTTCCTTACATTTCCACTGGTTTTTTCTGCATCCAATGATGGGAAGGTTAGAATTGGACTTAAAAGGAGGAATTTTGACCAGAACAACCGAGTTGCTGGCAAGATTGCAACCAAGGAATCAGTTTCCTTGAAAGGCTCTGAGAAATATCAGGCTAGTGGAAATTTAGGAGATTCGGCTGATACTGATATTGTAGCATTGAAGAATTACTTGAATGCTCAGTATTTTGGGGAGATTGGTATTGGCACTCCCCCTCAGAAGTTTACTGTTGTTTTTGACACTGGTAGTTCTAATTTATGGGTTCCATCATCTAAGTGCCTCTCTGTTGCATGTCTTCTCCATTCCAAGTACCGGTCGAGGCATTCAAGCACTTACAAGAAGAATGGAAAACGTGCCTCGATTAAATATGGTACTGGTGCTATTTCTGGCTACTTTAGTCAAGACCATGTTAAAGTTGGCAACCTAGTAGTTCAGAAACAGGATTTTATCGAGGCAACTAGGGAGCCCAGCCTCACATTTGTACTGGCCCAATTTGATGGCATTTTTGGACTTGGCTTCAAAGAGATTTCAGTTGGAGATGCAGTGCCTGTATGGTACAACATGGTTGACCAAAATCTTGTAAAGGAACTTGTTTTCTCTTTTTGGTTTAATCGCAATGTGGATGCGGATGAGGAACAAGGTGGTGAAATTGTATTTGGTGGTGTGGACCCTTATCACTACAAGGGGGAGCATACATATGTTCCAGTTACTAAAAAAGGATATTGGCAGTTTGATATGGGCGACGTTCTGATCAATGGCAGTACAACCGAATTTTGTTCAAATGGTTGTTCAGCCATTGCTGATTCAGGAACTTCTTTATTGGCTGGTCCAACGACTATTATCACTCAAATTAATCATGCTATTGGAGCCTCCGGGGTTGTAAGTGAGGAATGCAAGACTGTTGTGGCCGAATATGGTGAAACCATAATTAAAATGCTATTAGCAAAGGATCAACCAAAGAAAATCTGCTCTACACTTGGTTTGTGTACTTTTGACGGGACTCGAGGAGTGAGTATGGGCATCAAGAGCGTTGTTGATAACACCACTCAGAAATCTTCGAATGGTTTGCGTGATATTCTGTGCAACACATGTGAGATGGCAGTTGTATGGGCACAAAGCCAGCTAAAAGATGAACAAACACAAGACCAGATTCTCAATTATATGGATCGGCTGTGCGAGAAGTTGCCTAGTCCAATGGGAGAATCAGTAATAGATTGCGATTCCTTGTCTACTTTGCCTAATGTTTCATTTACAATTGGTGGAAAGGTCTTTGAGCTAAAGCCTGAGCAGTACGTCCTCAAAGTCACACAGGGACCTGTAACCGAATGCATCAGTGGATTTGCAGCATTGGATGTGCCGCCACCACGTGGACCTCTCTGGATATTGGGGGATGTATTCATGGGTCCATACCACACGGTGTTTGACTATGGAAACTTGAGAGTTGGGTTTGCAGAATCTGCTTAA

Protein sequence

MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKGSEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCLSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEATREPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQGGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSLLAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDGTRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCEKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVPPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA
Homology
BLAST of Sed0026314 vs. NCBI nr
Match: XP_023553350.1 (aspartic proteinase A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 936.0 bits (2418), Expect = 1.4e-268
Identity = 461/514 (89.69%), Postives = 485/514 (94.36%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MGT LKLFTA+LFICFL FP+VFSASNDGKVRIGLKRR F QN RVA KIATKE VSLK 
Sbjct: 1   MGTRLKLFTALLFICFLMFPMVFSASNDGKVRIGLKRRKFGQNIRVASKIATKEGVSLKN 60

Query: 61  SEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC 120
           S +      LGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTV+FDTGSSNLWVPSSKC
Sbjct: 61  SVE-----KLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 120

Query: 121 LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEAT 180
            SVACLLHSKY+S+ SSTY+KNGK ASI+YGTGAISGYFS+DHVKVG+L+V+KQ+FIEAT
Sbjct: 121 FSVACLLHSKYKSKRSSTYEKNGKHASIQYGTGAISGYFSEDHVKVGDLIVKKQEFIEAT 180

Query: 181 REPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQ 240
           +EPSLTFVLAQFDGI GLGFKEISVGDAVPVWYNMVDQNLVKE VFSFWFNRN   DEEQ
Sbjct: 181 KEPSLTFVLAQFDGILGLGFKEISVGDAVPVWYNMVDQNLVKEPVFSFWFNRN--EDEEQ 240

Query: 241 GGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSL 300
           GGEIVFGGVDP HYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCS GCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPNHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSGGCSAIADSGTSL 300

Query: 301 LAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 360
           LAGPT+IITQ+NHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG
Sbjct: 301 LAGPTSIITQVNHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 360

Query: 361 TRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCE 420
           TRGVSMGI+SVVDNTTQKSSNGL D++CN CEMAVVWAQSQLKDE+TQDQILNY+D LCE
Sbjct: 361 TRGVSMGIESVVDNTTQKSSNGLHDVMCNACEMAVVWAQSQLKDEKTQDQILNYIDGLCE 420

Query: 421 KLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVP 480
           KLPSPMGESVIDCDSLSTLP+VSFTIGGKVF+LKPEQYVLKVTQGPVTECISGFAALDVP
Sbjct: 421 KLPSPMGESVIDCDSLSTLPSVSFTIGGKVFDLKPEQYVLKVTQGPVTECISGFAALDVP 480

Query: 481 PPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           PPRGPLWILGDVFMG YHTVFDYGNLRVGFAE+A
Sbjct: 481 PPRGPLWILGDVFMGTYHTVFDYGNLRVGFAEAA 507

BLAST of Sed0026314 vs. NCBI nr
Match: KAG7011412.1 (Aspartic proteinase A1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 935.3 bits (2416), Expect = 2.3e-268
Identity = 461/514 (89.69%), Postives = 484/514 (94.16%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MGT LKLFTAVLFICFL FP+VFSA NDGKVRIGLKRR F QN RVA KIATKE VSLK 
Sbjct: 1   MGTRLKLFTAVLFICFLMFPMVFSAPNDGKVRIGLKRRKFGQNIRVASKIATKEGVSLKN 60

Query: 61  SEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC 120
           S +      LGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTV+FDTGSSNLWVPSSKC
Sbjct: 61  SVE-----KLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 120

Query: 121 LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEAT 180
            SVACLLHSKY+S+ SSTY+KNGK ASI+YGTGAISGYFS+DHVKVG+L+V+KQ+FIEAT
Sbjct: 121 FSVACLLHSKYKSKRSSTYEKNGKHASIQYGTGAISGYFSEDHVKVGDLIVKKQEFIEAT 180

Query: 181 REPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQ 240
           +EPSLTFVLAQFDGI GLGFKEISVGDAVPVWYNMVDQNLVKE VFSFWFNRN   DEEQ
Sbjct: 181 KEPSLTFVLAQFDGILGLGFKEISVGDAVPVWYNMVDQNLVKEPVFSFWFNRN--EDEEQ 240

Query: 241 GGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSL 300
           GGEIVFGGVDP HYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCS GCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPNHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSGGCSAIADSGTSL 300

Query: 301 LAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 360
           LAGPT+IITQ+NHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG
Sbjct: 301 LAGPTSIITQVNHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 360

Query: 361 TRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCE 420
           TRGVSMGI+SVVDNTTQKSSNGL D++CN CEMAVVWAQSQLKDE+TQDQILNY+D LCE
Sbjct: 361 TRGVSMGIESVVDNTTQKSSNGLHDVMCNACEMAVVWAQSQLKDEKTQDQILNYIDGLCE 420

Query: 421 KLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVP 480
           KLPSPMGESVIDCDSLSTLP+VSFTIGGKVF+LKPEQYVLKVTQGPVTECISGFAALDVP
Sbjct: 421 KLPSPMGESVIDCDSLSTLPSVSFTIGGKVFDLKPEQYVLKVTQGPVTECISGFAALDVP 480

Query: 481 PPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           PPRGPLWILGDVFMG YHTVFDYGNLRVGFAE+A
Sbjct: 481 PPRGPLWILGDVFMGTYHTVFDYGNLRVGFAEAA 507

BLAST of Sed0026314 vs. NCBI nr
Match: XP_004151126.1 (aspartic proteinase A1 [Cucumis sativus] >XP_011650512.1 aspartic proteinase A1 [Cucumis sativus] >KAE8650121.1 hypothetical protein Csa_009507 [Cucumis sativus])

HSP 1 Score: 934.5 bits (2414), Expect = 3.9e-268
Identity = 460/515 (89.32%), Postives = 481/515 (93.40%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MGT LKLF AVLFICF  FP+VF ASNDGKVRIGLKRR F QNNRVA KIATKE +SLK 
Sbjct: 1   MGTRLKLFIAVLFICFFMFPMVFCASNDGKVRIGLKRRKFGQNNRVASKIATKEGISLKN 60

Query: 61  S-EKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSK 120
           S EKYQ S NLGDS D DIV LKNYLNAQYFGEIGIGTPPQKF V+FDTGSSNLWVPSSK
Sbjct: 61  SVEKYQPSANLGDSDDFDIVGLKNYLNAQYFGEIGIGTPPQKFAVIFDTGSSNLWVPSSK 120

Query: 121 CLSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEA 180
           C SVACLLHSKY+S+ SSTYKKNGK ASIKYGTGAISGYFS+D+VKVG+L+V+KQDFIEA
Sbjct: 121 CFSVACLLHSKYKSKRSSTYKKNGKSASIKYGTGAISGYFSEDNVKVGDLIVKKQDFIEA 180

Query: 181 TREPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEE 240
           TREPSLTFVLAQFDGI GLGFKEISVGDAVPVWYNMVDQNLVKE VFSFWFNRN  ADEE
Sbjct: 181 TREPSLTFVLAQFDGILGLGFKEISVGDAVPVWYNMVDQNLVKEPVFSFWFNRN--ADEE 240

Query: 241 QGGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTS 300
           QGGEIVFGGVDP HYKGEHTYVPVTKKGYWQFDMGDVLINGSTT FCS GCSAIADSGTS
Sbjct: 241 QGGEIVFGGVDPDHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTGFCSGGCSAIADSGTS 300

Query: 301 LLAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFD 360
           LLAGPTTIITQ+NHAIGASGVVSEECK VVAEYGETIIKMLLAKDQPKKICSTLGLC FD
Sbjct: 301 LLAGPTTIITQVNHAIGASGVVSEECKAVVAEYGETIIKMLLAKDQPKKICSTLGLCAFD 360

Query: 361 GTRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLC 420
           G RGVSMGI+SVVDNTTQKSSNGLRD++CN CEMAVVWAQSQLK+E+TQDQILNY+D LC
Sbjct: 361 GERGVSMGIESVVDNTTQKSSNGLRDVMCNACEMAVVWAQSQLKEEKTQDQILNYIDGLC 420

Query: 421 EKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDV 480
           EKLPSPMGESVIDCDSLSTLP++SFTIGGKVFELKPEQYVLKVT+GPVTECISGFAALDV
Sbjct: 421 EKLPSPMGESVIDCDSLSTLPSISFTIGGKVFELKPEQYVLKVTEGPVTECISGFAALDV 480

Query: 481 PPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           PPPRGPLWILGDVFMG YHTVFDYGN RVGFAE+A
Sbjct: 481 PPPRGPLWILGDVFMGSYHTVFDYGNSRVGFAEAA 513

BLAST of Sed0026314 vs. NCBI nr
Match: XP_022932233.1 (aspartic proteinase A1-like [Cucurbita moschata])

HSP 1 Score: 934.1 bits (2413), Expect = 5.2e-268
Identity = 460/514 (89.49%), Postives = 484/514 (94.16%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MGT LKLFTA+LFICFL FP+VFSA NDGKVRIGLKRR F QN RVA KIATKE VSLK 
Sbjct: 1   MGTRLKLFTALLFICFLMFPMVFSAPNDGKVRIGLKRRKFGQNVRVASKIATKEEVSLKN 60

Query: 61  SEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC 120
           S +      LGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTV+FDTGSSNLWVPSSKC
Sbjct: 61  SVE-----KLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 120

Query: 121 LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEAT 180
            SVACLLHSKY+S+ SSTY+KNGK ASI+YGTGAISGYFS+DHVKVG+L+V+KQ+FIEAT
Sbjct: 121 FSVACLLHSKYKSKRSSTYEKNGKHASIQYGTGAISGYFSEDHVKVGDLIVKKQEFIEAT 180

Query: 181 REPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQ 240
           +EPSLTFVLAQFDGI GLGFKEISVGDAVPVWYNMVDQNLVKE VFSFWFNRN   DEEQ
Sbjct: 181 KEPSLTFVLAQFDGILGLGFKEISVGDAVPVWYNMVDQNLVKEPVFSFWFNRN--EDEEQ 240

Query: 241 GGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSL 300
           GGEIVFGGVDP HYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCS GCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPNHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSGGCSAIADSGTSL 300

Query: 301 LAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 360
           LAGPT+IITQ+NHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG
Sbjct: 301 LAGPTSIITQVNHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 360

Query: 361 TRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCE 420
           TRGVSMGI+SVVDNTTQKSSNGL D++CN CEMAVVWAQSQLKDE+TQDQILNY+D LCE
Sbjct: 361 TRGVSMGIESVVDNTTQKSSNGLHDVMCNACEMAVVWAQSQLKDEKTQDQILNYIDGLCE 420

Query: 421 KLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVP 480
           KLPSPMGESVIDCDSLSTLP+VSFTIGGKVF+LKPEQYVLKVTQGPVTECISGFAALDVP
Sbjct: 421 KLPSPMGESVIDCDSLSTLPSVSFTIGGKVFDLKPEQYVLKVTQGPVTECISGFAALDVP 480

Query: 481 PPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           PPRGPLWILGDVFMG YHTVFDYGNLRVGFAE+A
Sbjct: 481 PPRGPLWILGDVFMGTYHTVFDYGNLRVGFAEAA 507

BLAST of Sed0026314 vs. NCBI nr
Match: XP_023007789.1 (aspartic proteinase A1-like [Cucurbita maxima])

HSP 1 Score: 933.3 bits (2411), Expect = 8.8e-268
Identity = 459/514 (89.30%), Postives = 484/514 (94.16%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MGT +KLFTA+LFICFL FP+VFSASNDGKVRIGLKRR F QN RVA KIATKE VSLK 
Sbjct: 1   MGTRIKLFTALLFICFLMFPMVFSASNDGKVRIGLKRRKFGQNIRVASKIATKEGVSLKN 60

Query: 61  SEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC 120
           S +      LGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTV+FDTGSSNLWVPSSKC
Sbjct: 61  SVE-----KLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 120

Query: 121 LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEAT 180
            SVACLLHSKY+S+ SSTY+KNGK ASI+YGTGAISGYFS+DHVKVG+L+V+KQ+FIEAT
Sbjct: 121 FSVACLLHSKYKSKRSSTYEKNGKHASIQYGTGAISGYFSEDHVKVGDLIVKKQEFIEAT 180

Query: 181 REPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQ 240
           +EPSLTFVLAQFDGI GLGFKEISVGDAVPVWYNMVDQNLVKE VFSFWFNRN   DEEQ
Sbjct: 181 KEPSLTFVLAQFDGILGLGFKEISVGDAVPVWYNMVDQNLVKEPVFSFWFNRN--EDEEQ 240

Query: 241 GGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSL 300
           GGEIVFGGVDP HYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCS GCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPNHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSGGCSAIADSGTSL 300

Query: 301 LAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 360
           LAGPT+IITQ+NHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLC FDG
Sbjct: 301 LAGPTSIITQVNHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCAFDG 360

Query: 361 TRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCE 420
           TRGVSMGI+SVVDNTTQKSSNGL D++CN CEMAVVWAQSQLKDE+TQDQILNY+D LCE
Sbjct: 361 TRGVSMGIESVVDNTTQKSSNGLHDVMCNACEMAVVWAQSQLKDEKTQDQILNYIDGLCE 420

Query: 421 KLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVP 480
           KLPSPMGESVIDCDSLSTLP+VSFTIGGKVF+LKPEQYVLKVTQGPVTECISGFAALDVP
Sbjct: 421 KLPSPMGESVIDCDSLSTLPSVSFTIGGKVFDLKPEQYVLKVTQGPVTECISGFAALDVP 480

Query: 481 PPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           PPRGPLWILGDVFMG YHTVFDYGNLRVGFAE+A
Sbjct: 481 PPRGPLWILGDVFMGTYHTVFDYGNLRVGFAEAA 507

BLAST of Sed0026314 vs. ExPASy Swiss-Prot
Match: O04057 (Aspartic proteinase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 8.1e-208
Identity = 341/506 (67.39%), Postives = 423/506 (83.60%), Query Frame = 0

Query: 13  FIC---FLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKGS-EKYQASG 72
           F+C    ++F +V SASNDG +R+GLK+   D  NR+A ++ +K++  LK +  KY   G
Sbjct: 10  FLCLFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPKG 69

Query: 73  NLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCLSVACLLH 132
           NLG+S+DTDIVALKNYL+AQY+GEI IGTPPQKFTV+FDTGSSNLWV      SVAC  H
Sbjct: 70  NLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVLCECLFSVACHFH 129

Query: 133 SKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEATREPSLTFV 192
           ++Y+S  SS+YKKNG  ASI+YGTGA+SG+FS D+VKVG+LVV++Q FIEATREPSLTF+
Sbjct: 130 ARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATREPSLTFL 189

Query: 193 LAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQGGEIVFGG 252
           +A+FDG+ GLGF+EI+VG+AVPVWYNMV+Q LVKE VFSFW NRNV  +EE+GGEIVFGG
Sbjct: 190 VAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNV--EEEEGGEIVFGG 249

Query: 253 VDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSLLAGPTTII 312
           VDP HY+G+HTYVPVT+KGYWQFDMGDVLI+G  T FC  GCSAIADSGTSLLAGPT +I
Sbjct: 250 VDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAGPTPVI 309

Query: 313 TQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDGTRGVSMGI 372
           T INHAIGA GVVS++CK VVA+YG+TI+ +LL++  PKKICS + LCTFDGTRGVSMGI
Sbjct: 310 TMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGI 369

Query: 373 KSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCEKLPSPMGE 432
           +SVVD    KSS+ L D +C+ CEM VVW Q+QL+  QT+++I+NY++ LC+++PSPMG+
Sbjct: 370 ESVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQ 429

Query: 433 SVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVPPPRGPLWI 492
           S +DC  LS++P VSFTIGGK+F+L PE+Y+LKV +GPV +CISGF A D+PPPRGPLWI
Sbjct: 430 SAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWI 489

Query: 493 LGDVFMGPYHTVFDYGNLRVGFAESA 515
           LGDVFMG YHTVFD+G LRVG AE+A
Sbjct: 490 LGDVFMGRYHTVFDFGKLRVGSAEAA 513

BLAST of Sed0026314 vs. ExPASy Swiss-Prot
Match: O65390 (Aspartic proteinase A1 OS=Arabidopsis thaliana OX=3702 GN=APA1 PE=1 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 1.4e-207
Identity = 349/504 (69.25%), Postives = 413/504 (81.94%), Query Frame = 0

Query: 12  LFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKGSEKYQASGNLG 71
           L + FL     F+  NDG  R+GLK+   D  NR+A ++ +K+       EK   +  LG
Sbjct: 12  LIVSFLLCFSAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQ-------EKPLRAYRLG 71

Query: 72  DSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC-LSVACLLHSK 131
           DS D D+V LKNYL+AQY+GEI IGTPPQKFTVVFDTGSSNLWVPSSKC  S+ACLLH K
Sbjct: 72  DSGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSLACLLHPK 131

Query: 132 YRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEATREPSLTFVLA 191
           Y+S  SSTY+KNGK A+I YGTGAI+G+FS D V VG+LVV+ Q+FIEAT+EP +TFV+A
Sbjct: 132 YKSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEPGITFVVA 191

Query: 192 QFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQGGEIVFGGVD 251
           +FDGI GLGF+EISVG A PVWYNM+ Q L+KE VFSFW NRN  ADEE+GGE+VFGGVD
Sbjct: 192 KFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRN--ADEEEGGELVFGGVD 251

Query: 252 PYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSLLAGPTTIITQ 311
           P H+KG+HTYVPVT+KGYWQFDMGDVLI G+ T FC +GCSAIADSGTSLLAGPTTIIT 
Sbjct: 252 PNHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPTTIITM 311

Query: 312 INHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDGTRGVSMGIKS 371
           INHAIGA+GVVS++CKTVV +YG+TI+ +LL++ QPKKICS +GLCTFDGTRGVSMGI+S
Sbjct: 312 INHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVSMGIES 371

Query: 372 VVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCEKLPSPMGESV 431
           VVD    K SNG+ D  C+ CEMAVVW QSQL+   TQ++ILNY++ LCE+LPSPMGES 
Sbjct: 372 VVDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMGESA 431

Query: 432 IDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVPPPRGPLWILG 491
           +DC  LST+P VS TIGGKVF+L PE+YVLKV +GPV +CISGF ALDV PPRGPLWILG
Sbjct: 432 VDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLWILG 491

Query: 492 DVFMGPYHTVFDYGNLRVGFAESA 515
           DVFMG YHTVFD+GN +VGFAE+A
Sbjct: 492 DVFMGKYHTVFDFGNEQVGFAEAA 506

BLAST of Sed0026314 vs. ExPASy Swiss-Prot
Match: Q8VYL3 (Aspartic proteinase A2 OS=Arabidopsis thaliana OX=3702 GN=APA2 PE=2 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 3.1e-199
Identity = 327/514 (63.62%), Postives = 409/514 (79.57%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MG   +     +F+ FL F   +S  NDG  R+GLK+   D NNR+A +  +K+  +L+ 
Sbjct: 1   MGVYSRAVAFSVFVSFLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRS 60

Query: 61  SEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC 120
           S +   +   GDS D DIV LKNYL+AQY+GEI IGTPPQKFTV+FDTGSSNLWVPS KC
Sbjct: 61  SLRSYNNNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKC 120

Query: 121 -LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEA 180
             S++C  H+KY+S  SSTYKK+GKRA+I YG+G+ISG+FS D V VG+LVV+ Q+FIE 
Sbjct: 121 FFSLSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIET 180

Query: 181 TREPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEE 240
           T EP LTF++A+FDG+ GLGF+EI+VG+A PVWYNM+ Q L+K  VFSFW NR  D   E
Sbjct: 181 TSEPGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNR--DPKSE 240

Query: 241 QGGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTS 300
           +GGEIVFGGVDP H++GEHT+VPVT++GYWQFDMG+VLI G +T +C +GCSAIADSGTS
Sbjct: 241 EGGEIVFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTS 300

Query: 301 LLAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFD 360
           LLAGPT ++  IN AIGASGVVS++CKTVV +YG+TI+ +LLA+ QPKKICS +GLC +D
Sbjct: 301 LLAGPTAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYD 360

Query: 361 GTRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLC 420
           GT GVSMGI+SVVD    +SS+GLRD  C  CEMAVVW QSQL+   TQ++I+NY++ +C
Sbjct: 361 GTHGVSMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEIC 420

Query: 421 EKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDV 480
           E++PSP GES +DC  LS +P VSFTIGGKVF+L PE+YVLK+ +GPV +CISGF ALD+
Sbjct: 421 ERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDI 480

Query: 481 PPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAES 514
           PPPRGPLWILGDVFMG YHTVFD+GN +VGFAE+
Sbjct: 481 PPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

BLAST of Sed0026314 vs. ExPASy Swiss-Prot
Match: Q42456 (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 694.1 bits (1790), Expect = 1.2e-198
Identity = 333/512 (65.04%), Postives = 413/512 (80.66%), Query Frame = 0

Query: 4   GLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKGSEK 63
           G +    VL    L   L+ +++ +G VRI LK+R  D+N+RVA +++ +E     G   
Sbjct: 2   GTRSVALVLLAAVLLQALLPASAAEGLVRIALKKRPIDENSRVAARLSGEEGARRLGLRG 61

Query: 64  YQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC-LS 123
             + G  G   + DIVALKNY+NAQYFGEIG+GTPPQKFTV+FDTGSSNLWVPS+KC  S
Sbjct: 62  ANSLG--GGGGEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFS 121

Query: 124 VACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEATRE 183
           +AC  HS+Y+S  SSTY+KNGK A+I+YGTG+I+G+FS+D V VG+LVV+ Q+FIEAT+E
Sbjct: 122 IACFFHSRYKSGQSSTYQKNGKPAAIQYGTGSIAGFFSEDSVTVGDLVVKDQEFIEATKE 181

Query: 184 PSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQGG 243
           P LTF++A+FDGI GLGF+EISVGDAVPVWY MV+Q LV E VFSFWFNR+  +DE +GG
Sbjct: 182 PGLTFMVAKFDGILGLGFQEISVGDAVPVWYKMVEQGLVSEPVFSFWFNRH--SDEGEGG 241

Query: 244 EIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSLLA 303
           EIVFGG+DP HYKG HTYVPV++KGYWQF+MGDVLI G TT FC++GCSAIADSGTSLLA
Sbjct: 242 EIVFGGMDPSHYKGNHTYVPVSQKGYWQFEMGDVLIGGKTTGFCASGCSAIADSGTSLLA 301

Query: 304 GPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDGTR 363
           GPT IIT+IN  IGA+GVVS+ECKTVV++YG+ I+ +LLA+ QP KICS +GLCTFDG  
Sbjct: 302 GPTAIITEINEKIGATGVVSQECKTVVSQYGQQILDLLLAETQPSKICSQVGLCTFDGKH 361

Query: 364 GVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCEKL 423
           GVS GIKSVVD+   +S+      +CN CEMAVVW Q+QL   +TQD ILNY+++LC+KL
Sbjct: 362 GVSAGIKSVVDDEAGESNGLQSGPMCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKL 421

Query: 424 PSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVPPP 483
           PSPMGES +DC SL+++P +SFTIGGK F LKPE+Y+LKV +G   +CISGF A+D+PPP
Sbjct: 422 PSPMGESSVDCGSLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPP 481

Query: 484 RGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           RGPLWILGDVFMG YHTVFDYG +RVGFA+SA
Sbjct: 482 RGPLWILGDVFMGAYHTVFDYGKMRVGFAKSA 509

BLAST of Sed0026314 vs. ExPASy Swiss-Prot
Match: P42210 (Phytepsin OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 2.9e-197
Identity = 335/515 (65.05%), Postives = 414/515 (80.39%), Query Frame = 0

Query: 2   GTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKGS 61
           G  L L  AVL    L   L  ++  +G VRI LK+R  D+N+RVA  ++  E   L   
Sbjct: 5   GLALALLAAVL---LLQTVLPAASEAEGLVRIALKKRPIDRNSRVATGLSGGEEQPL--- 64

Query: 62  EKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC- 121
                +  L    + DIVALKNY+NAQYFGEIG+GTPPQKFTV+FDTGSSNLWVPS+KC 
Sbjct: 65  --LSGANPLRSEEEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCY 124

Query: 122 LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEAT 181
            S+AC LHS+Y++  SSTYKKNGK A+I+YGTG+I+GYFS+D V VG+LVV+ Q+FIEAT
Sbjct: 125 FSIACYLHSRYKAGASSTYKKNGKPAAIQYGTGSIAGYFSEDSVTVGDLVVKDQEFIEAT 184

Query: 182 REPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQ 241
           +EP +TF++A+FDGI GLGFKEISVG AVPVWY M++Q LV + VFSFW NR+V  DE +
Sbjct: 185 KEPGITFLVAKFDGILGLGFKEISVGKAVPVWYKMIEQGLVSDPVFSFWLNRHV--DEGE 244

Query: 242 GGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSL 301
           GGEI+FGG+DP HY GEHTYVPVT+KGYWQFDMGDVL+ G +T FC+ GC+AIADSGTSL
Sbjct: 245 GGEIIFGGMDPKHYVGEHTYVPVTQKGYWQFDMGDVLVGGKSTGFCAGGCAAIADSGTSL 304

Query: 302 LAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 361
           LAGPT IIT+IN  IGA+GVVS+ECKT+V++YG+ I+ +LLA+ QPKKICS +GLCTFDG
Sbjct: 305 LAGPTAIITEINEKIGAAGVVSQECKTIVSQYGQQILDLLLAETQPKKICSQVGLCTFDG 364

Query: 362 TRGVSMGIKSVVDNTTQKSSNGLR-DILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLC 421
           TRGVS GI+SVVD+   K SNGLR D +C+ CEMAVVW Q+QL   +TQD IL+Y+++LC
Sbjct: 365 TRGVSAGIRSVVDDEPVK-SNGLRADPMCSACEMAVVWMQNQLAQNKTQDLILDYVNQLC 424

Query: 422 EKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDV 481
            +LPSPMGES +DC SL ++P++ FTIGGK F LKPE+Y+LKV +G   +CISGF A+D+
Sbjct: 425 NRLPSPMGESAVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDI 484

Query: 482 PPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           PPPRGPLWILGDVFMGPYHTVFDYG LR+GFA++A
Sbjct: 485 PPPRGPLWILGDVFMGPYHTVFDYGKLRIGFAKAA 508

BLAST of Sed0026314 vs. ExPASy TrEMBL
Match: A0A6J1F137 (aspartic proteinase A1-like OS=Cucurbita moschata OX=3662 GN=LOC111438595 PE=3 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 2.5e-268
Identity = 460/514 (89.49%), Postives = 484/514 (94.16%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MGT LKLFTA+LFICFL FP+VFSA NDGKVRIGLKRR F QN RVA KIATKE VSLK 
Sbjct: 1   MGTRLKLFTALLFICFLMFPMVFSAPNDGKVRIGLKRRKFGQNVRVASKIATKEEVSLKN 60

Query: 61  SEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC 120
           S +      LGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTV+FDTGSSNLWVPSSKC
Sbjct: 61  SVE-----KLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 120

Query: 121 LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEAT 180
            SVACLLHSKY+S+ SSTY+KNGK ASI+YGTGAISGYFS+DHVKVG+L+V+KQ+FIEAT
Sbjct: 121 FSVACLLHSKYKSKRSSTYEKNGKHASIQYGTGAISGYFSEDHVKVGDLIVKKQEFIEAT 180

Query: 181 REPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQ 240
           +EPSLTFVLAQFDGI GLGFKEISVGDAVPVWYNMVDQNLVKE VFSFWFNRN   DEEQ
Sbjct: 181 KEPSLTFVLAQFDGILGLGFKEISVGDAVPVWYNMVDQNLVKEPVFSFWFNRN--EDEEQ 240

Query: 241 GGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSL 300
           GGEIVFGGVDP HYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCS GCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPNHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSGGCSAIADSGTSL 300

Query: 301 LAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 360
           LAGPT+IITQ+NHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG
Sbjct: 301 LAGPTSIITQVNHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 360

Query: 361 TRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCE 420
           TRGVSMGI+SVVDNTTQKSSNGL D++CN CEMAVVWAQSQLKDE+TQDQILNY+D LCE
Sbjct: 361 TRGVSMGIESVVDNTTQKSSNGLHDVMCNACEMAVVWAQSQLKDEKTQDQILNYIDGLCE 420

Query: 421 KLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVP 480
           KLPSPMGESVIDCDSLSTLP+VSFTIGGKVF+LKPEQYVLKVTQGPVTECISGFAALDVP
Sbjct: 421 KLPSPMGESVIDCDSLSTLPSVSFTIGGKVFDLKPEQYVLKVTQGPVTECISGFAALDVP 480

Query: 481 PPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           PPRGPLWILGDVFMG YHTVFDYGNLRVGFAE+A
Sbjct: 481 PPRGPLWILGDVFMGTYHTVFDYGNLRVGFAEAA 507

BLAST of Sed0026314 vs. ExPASy TrEMBL
Match: A0A6J1L5Y5 (aspartic proteinase A1-like OS=Cucurbita maxima OX=3661 GN=LOC111500293 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.3e-268
Identity = 459/514 (89.30%), Postives = 484/514 (94.16%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MGT +KLFTA+LFICFL FP+VFSASNDGKVRIGLKRR F QN RVA KIATKE VSLK 
Sbjct: 1   MGTRIKLFTALLFICFLMFPMVFSASNDGKVRIGLKRRKFGQNIRVASKIATKEGVSLKN 60

Query: 61  SEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC 120
           S +      LGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTV+FDTGSSNLWVPSSKC
Sbjct: 61  SVE-----KLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 120

Query: 121 LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEAT 180
            SVACLLHSKY+S+ SSTY+KNGK ASI+YGTGAISGYFS+DHVKVG+L+V+KQ+FIEAT
Sbjct: 121 FSVACLLHSKYKSKRSSTYEKNGKHASIQYGTGAISGYFSEDHVKVGDLIVKKQEFIEAT 180

Query: 181 REPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQ 240
           +EPSLTFVLAQFDGI GLGFKEISVGDAVPVWYNMVDQNLVKE VFSFWFNRN   DEEQ
Sbjct: 181 KEPSLTFVLAQFDGILGLGFKEISVGDAVPVWYNMVDQNLVKEPVFSFWFNRN--EDEEQ 240

Query: 241 GGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSL 300
           GGEIVFGGVDP HYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCS GCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPNHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSGGCSAIADSGTSL 300

Query: 301 LAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDG 360
           LAGPT+IITQ+NHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLC FDG
Sbjct: 301 LAGPTSIITQVNHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCAFDG 360

Query: 361 TRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCE 420
           TRGVSMGI+SVVDNTTQKSSNGL D++CN CEMAVVWAQSQLKDE+TQDQILNY+D LCE
Sbjct: 361 TRGVSMGIESVVDNTTQKSSNGLHDVMCNACEMAVVWAQSQLKDEKTQDQILNYIDGLCE 420

Query: 421 KLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVP 480
           KLPSPMGESVIDCDSLSTLP+VSFTIGGKVF+LKPEQYVLKVTQGPVTECISGFAALDVP
Sbjct: 421 KLPSPMGESVIDCDSLSTLPSVSFTIGGKVFDLKPEQYVLKVTQGPVTECISGFAALDVP 480

Query: 481 PPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           PPRGPLWILGDVFMG YHTVFDYGNLRVGFAE+A
Sbjct: 481 PPRGPLWILGDVFMGTYHTVFDYGNLRVGFAEAA 507

BLAST of Sed0026314 vs. ExPASy TrEMBL
Match: A0A5D3DKC0 (Aspartic proteinase A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00070 PE=3 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 5.2e-266
Identity = 460/518 (88.80%), Postives = 481/518 (92.86%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           M T LKLF AVLFICFL FP+VF ASNDGKVRIGLKRR F QN+RVA KIATKE +SLK 
Sbjct: 1   MSTRLKLFIAVLFICFLMFPMVFCASNDGKVRIGLKRRKFGQNHRVASKIATKEGISLKN 60

Query: 61  -SEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSK 120
             EKYQ S NLGDS D DIV LKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSK
Sbjct: 61  YVEKYQPSANLGDSDDFDIVGLKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSK 120

Query: 121 CLSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEA 180
           C SVACLLHSKY+S+ SSTYKKNGK ASIKYGTGAISGYFS+D+VKVG+L+V+KQDFIEA
Sbjct: 121 CFSVACLLHSKYKSKRSSTYKKNGKPASIKYGTGAISGYFSEDNVKVGDLIVKKQDFIEA 180

Query: 181 TREPSLTFVLAQFDGIFGLGFKEISVGDAVPVW---YNMVDQNLVKELVFSFWFNRNVDA 240
           TREPSLTFVLAQFDGI GLGFKEISVGDAVPVW   Y+MVDQNLVKE VFSFWFNRN  A
Sbjct: 181 TREPSLTFVLAQFDGILGLGFKEISVGDAVPVWYYRYSMVDQNLVKEPVFSFWFNRN--A 240

Query: 241 DEEQGGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADS 300
           DEEQGGEIVFGGVDP HYKGEHTYVPVTKKGYWQFDMGDVLINGSTT FCS GCSAIADS
Sbjct: 241 DEEQGGEIVFGGVDPDHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTGFCSGGCSAIADS 300

Query: 301 GTSLLAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLC 360
           GTSLLAGPTTIITQ+NHAIGASGVVSEECK VVAEYGETIIKMLLAKDQPKKICSTLGLC
Sbjct: 301 GTSLLAGPTTIITQVNHAIGASGVVSEECKAVVAEYGETIIKMLLAKDQPKKICSTLGLC 360

Query: 361 TFDGTRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMD 420
            FDG RGVSMGI+SVVDNTTQKSSNGLRD++CN CEMAVVWAQSQLKDE+TQDQILNY+D
Sbjct: 361 AFDGERGVSMGIESVVDNTTQKSSNGLRDVMCNACEMAVVWAQSQLKDEKTQDQILNYID 420

Query: 421 RLCEKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAA 480
            LCEKLPSPMGESVIDCDSLSTLPN++FTIGGKVFELKPEQYVLKVT+GPVTECISGFAA
Sbjct: 421 GLCEKLPSPMGESVIDCDSLSTLPNIAFTIGGKVFELKPEQYVLKVTEGPVTECISGFAA 480

Query: 481 LDVPPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           LDVPPPRGPLWILGDVFMG YHTVFDYGN RVGFAE+A
Sbjct: 481 LDVPPPRGPLWILGDVFMGSYHTVFDYGNSRVGFAEAA 516

BLAST of Sed0026314 vs. ExPASy TrEMBL
Match: A0A6J1DCK4 (aspartic proteinase A1-like OS=Momordica charantia OX=3673 GN=LOC111019253 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.5e-262
Identity = 446/516 (86.43%), Postives = 480/516 (93.02%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MGT LKL TAVLFICFL  P+V SASND KVRIGLKRRNFDQ+NRVA KIATKE VSL+ 
Sbjct: 1   MGTRLKLLTAVLFICFLMLPMVISASNDDKVRIGLKRRNFDQHNRVASKIATKEGVSLRD 60

Query: 61  S-EKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSK 120
           S EKYQ S NLGDSADTDIVALKNY+NAQYFGEIGIGTPPQKFTV+FDTGSSNLWVPSSK
Sbjct: 61  SVEKYQPSENLGDSADTDIVALKNYMNAQYFGEIGIGTPPQKFTVIFDTGSSNLWVPSSK 120

Query: 121 CLSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEA 180
           CLSVACLLHSKY+SR SSTYKKNGKRASIKYGTGAISGYFS+DHVKVG+LVV+ QDFIEA
Sbjct: 121 CLSVACLLHSKYKSRRSSTYKKNGKRASIKYGTGAISGYFSRDHVKVGDLVVKNQDFIEA 180

Query: 181 TREPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEE 240
           TREPSLTF+LA FDGI GLGFKEISVGDAVPVWYNMVDQ LVKE VFSFWFNRN   DEE
Sbjct: 181 TREPSLTFLLAHFDGILGLGFKEISVGDAVPVWYNMVDQGLVKEPVFSFWFNRN--GDEE 240

Query: 241 QGGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTS 300
           +GGEIVFGGVDP HYKGEHTYVPVTKKGYWQFDMGDV ING TT FCSNGCSAIADSGTS
Sbjct: 241 EGGEIVFGGVDPNHYKGEHTYVPVTKKGYWQFDMGDVRINGKTTGFCSNGCSAIADSGTS 300

Query: 301 LLAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFD 360
           LLAGPT+IITQ+NHAIGASG+VSEECKT+V+EYGETIIK+LL K++PKKICSTLGLCTFD
Sbjct: 301 LLAGPTSIITQVNHAIGASGIVSEECKTLVSEYGETIIKLLLEKEEPKKICSTLGLCTFD 360

Query: 361 GTRGVSMGIKSVVDNTTQKSSNGLRD-ILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRL 420
           GTRGVS GI SV+DNTTQ +SNGL D ++C  CEM VVW QSQL DEQTQD+IL+Y++RL
Sbjct: 361 GTRGVSTGIASVLDNTTQTTSNGLHDSLMCTACEMVVVWIQSQLADEQTQDRILDYINRL 420

Query: 421 CEKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALD 480
           CEKLPSPMGESVIDCDSLSTLPN+SFTIGGKVF+LKPE+YV+KVTQGP+T+CISGFAALD
Sbjct: 421 CEKLPSPMGESVIDCDSLSTLPNISFTIGGKVFDLKPEEYVIKVTQGPITQCISGFAALD 480

Query: 481 VPPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           VPPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAE+A
Sbjct: 481 VPPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAEAA 514

BLAST of Sed0026314 vs. ExPASy TrEMBL
Match: A0A0A0L2W7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G066750 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 4.0e-242
Identity = 424/515 (82.33%), Postives = 444/515 (86.21%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MGT LKLF AVLFICF  FP+VF ASNDGKVRIGLKRR F QNNRVA KIATKE +SLK 
Sbjct: 61  MGTRLKLFIAVLFICFFMFPMVFCASNDGKVRIGLKRRKFGQNNRVASKIATKEGISLKN 120

Query: 61  S-EKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSK 120
           S EKYQ S NLGDS D DIV LKNYLNAQYFGEIGIGTPPQKF V+FDTGSSNLWVPSSK
Sbjct: 121 SVEKYQPSANLGDSDDFDIVGLKNYLNAQYFGEIGIGTPPQKFAVIFDTGSSNLWVPSSK 180

Query: 121 CLSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEA 180
           C SVACLLHSKY+S+ SSTYKKNGK ASIKYGTGAISGYFS+D+VKVG+L+V+KQDFIEA
Sbjct: 181 CFSVACLLHSKYKSKRSSTYKKNGKSASIKYGTGAISGYFSEDNVKVGDLIVKKQDFIEA 240

Query: 181 TREPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEE 240
           TREPSLTFVLAQFDGI GLGFKEISVGDAVPVWYNMVDQNLVKE VFSFWFNRN  ADEE
Sbjct: 241 TREPSLTFVLAQFDGILGLGFKEISVGDAVPVWYNMVDQNLVKEPVFSFWFNRN--ADEE 300

Query: 241 QGGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTS 300
           QGGEIVFGGVDP HYKGEHTYVPVTKKGYWQFDMGDVLINGSTT FCS GCSAIADSGTS
Sbjct: 301 QGGEIVFGGVDPDHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTGFCSGGCSAIADSGTS 360

Query: 301 LLAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFD 360
           LLAGPT                                      DQPKKICSTLGLC FD
Sbjct: 361 LLAGPT--------------------------------------DQPKKICSTLGLCAFD 420

Query: 361 GTRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLC 420
           G RGVSMGI+SVVDNTTQKSSNGLRD++CN CEMAVVWAQSQLK+E+TQDQILNY+D LC
Sbjct: 421 GERGVSMGIESVVDNTTQKSSNGLRDVMCNACEMAVVWAQSQLKEEKTQDQILNYIDGLC 480

Query: 421 EKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDV 480
           EKLPSPMGESVIDCDSLSTLP++SFTIGGKVFELKPEQYVLKVT+GPVTECISGFAALDV
Sbjct: 481 EKLPSPMGESVIDCDSLSTLPSISFTIGGKVFELKPEQYVLKVTEGPVTECISGFAALDV 535

Query: 481 PPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           PPPRGPLWILGDVFMG YHTVFDYGN RVGFAE+A
Sbjct: 541 PPPRGPLWILGDVFMGSYHTVFDYGNSRVGFAEAA 535

BLAST of Sed0026314 vs. TAIR 10
Match: AT1G11910.1 (aspartic proteinase A1 )

HSP 1 Score: 723.8 bits (1867), Expect = 9.9e-209
Identity = 349/504 (69.25%), Postives = 413/504 (81.94%), Query Frame = 0

Query: 12  LFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKGSEKYQASGNLG 71
           L + FL     F+  NDG  R+GLK+   D  NR+A ++ +K+       EK   +  LG
Sbjct: 12  LIVSFLLCFSAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQ-------EKPLRAYRLG 71

Query: 72  DSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC-LSVACLLHSK 131
           DS D D+V LKNYL+AQY+GEI IGTPPQKFTVVFDTGSSNLWVPSSKC  S+ACLLH K
Sbjct: 72  DSGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSLACLLHPK 131

Query: 132 YRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEATREPSLTFVLA 191
           Y+S  SSTY+KNGK A+I YGTGAI+G+FS D V VG+LVV+ Q+FIEAT+EP +TFV+A
Sbjct: 132 YKSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEPGITFVVA 191

Query: 192 QFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEEQGGEIVFGGVD 251
           +FDGI GLGF+EISVG A PVWYNM+ Q L+KE VFSFW NRN  ADEE+GGE+VFGGVD
Sbjct: 192 KFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRN--ADEEEGGELVFGGVD 251

Query: 252 PYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTSLLAGPTTIITQ 311
           P H+KG+HTYVPVT+KGYWQFDMGDVLI G+ T FC +GCSAIADSGTSLLAGPTTIIT 
Sbjct: 252 PNHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPTTIITM 311

Query: 312 INHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFDGTRGVSMGIKS 371
           INHAIGA+GVVS++CKTVV +YG+TI+ +LL++ QPKKICS +GLCTFDGTRGVSMGI+S
Sbjct: 312 INHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVSMGIES 371

Query: 372 VVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLCEKLPSPMGESV 431
           VVD    K SNG+ D  C+ CEMAVVW QSQL+   TQ++ILNY++ LCE+LPSPMGES 
Sbjct: 372 VVDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMGESA 431

Query: 432 IDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDVPPPRGPLWILG 491
           +DC  LST+P VS TIGGKVF+L PE+YVLKV +GPV +CISGF ALDV PPRGPLWILG
Sbjct: 432 VDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLWILG 491

Query: 492 DVFMGPYHTVFDYGNLRVGFAESA 515
           DVFMG YHTVFD+GN +VGFAE+A
Sbjct: 492 DVFMGKYHTVFDFGNEQVGFAEAA 506

BLAST of Sed0026314 vs. TAIR 10
Match: AT1G62290.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 696.0 bits (1795), Expect = 2.2e-200
Identity = 327/514 (63.62%), Postives = 409/514 (79.57%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MG   +     +F+ FL F   +S  NDG  R+GLK+   D NNR+A +  +K+  +L+ 
Sbjct: 1   MGVYSRAVAFSVFVSFLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRS 60

Query: 61  SEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC 120
           S +   +   GDS D DIV LKNYL+AQY+GEI IGTPPQKFTV+FDTGSSNLWVPS KC
Sbjct: 61  SLRSYNNNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKC 120

Query: 121 -LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEA 180
             S++C  H+KY+S  SSTYKK+GKRA+I YG+G+ISG+FS D V VG+LVV+ Q+FIE 
Sbjct: 121 FFSLSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIET 180

Query: 181 TREPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEE 240
           T EP LTF++A+FDG+ GLGF+EI+VG+A PVWYNM+ Q L+K  VFSFW NR  D   E
Sbjct: 181 TSEPGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNR--DPKSE 240

Query: 241 QGGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTS 300
           +GGEIVFGGVDP H++GEHT+VPVT++GYWQFDMG+VLI G +T +C +GCSAIADSGTS
Sbjct: 241 EGGEIVFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTS 300

Query: 301 LLAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFD 360
           LLAGPT ++  IN AIGASGVVS++CKTVV +YG+TI+ +LLA+ QPKKICS +GLC +D
Sbjct: 301 LLAGPTAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYD 360

Query: 361 GTRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLC 420
           GT GVSMGI+SVVD    +SS+GLRD  C  CEMAVVW QSQL+   TQ++I+NY++ +C
Sbjct: 361 GTHGVSMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEIC 420

Query: 421 EKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDV 480
           E++PSP GES +DC  LS +P VSFTIGGKVF+L PE+YVLK+ +GPV +CISGF ALD+
Sbjct: 421 ERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDI 480

Query: 481 PPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAES 514
           PPPRGPLWILGDVFMG YHTVFD+GN +VGFAE+
Sbjct: 481 PPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

BLAST of Sed0026314 vs. TAIR 10
Match: AT1G62290.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 696.0 bits (1795), Expect = 2.2e-200
Identity = 327/514 (63.62%), Postives = 409/514 (79.57%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSASNDGKVRIGLKRRNFDQNNRVAGKIATKESVSLKG 60
           MG   +     +F+ FL F   +S  NDG  R+GLK+   D NNR+A +  +K+  +L+ 
Sbjct: 1   MGVYSRAVAFSVFVSFLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRS 60

Query: 61  SEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVPSSKC 120
           S +   +   GDS D DIV LKNYL+AQY+GEI IGTPPQKFTV+FDTGSSNLWVPS KC
Sbjct: 61  SLRSYNNNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKC 120

Query: 121 -LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQDFIEA 180
             S++C  H+KY+S  SSTYKK+GKRA+I YG+G+ISG+FS D V VG+LVV+ Q+FIE 
Sbjct: 121 FFSLSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIET 180

Query: 181 TREPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVDADEE 240
           T EP LTF++A+FDG+ GLGF+EI+VG+A PVWYNM+ Q L+K  VFSFW NR  D   E
Sbjct: 181 TSEPGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNR--DPKSE 240

Query: 241 QGGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIADSGTS 300
           +GGEIVFGGVDP H++GEHT+VPVT++GYWQFDMG+VLI G +T +C +GCSAIADSGTS
Sbjct: 241 EGGEIVFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTS 300

Query: 301 LLAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGLCTFD 360
           LLAGPT ++  IN AIGASGVVS++CKTVV +YG+TI+ +LLA+ QPKKICS +GLC +D
Sbjct: 301 LLAGPTAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYD 360

Query: 361 GTRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYMDRLC 420
           GT GVSMGI+SVVD    +SS+GLRD  C  CEMAVVW QSQL+   TQ++I+NY++ +C
Sbjct: 361 GTHGVSMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEIC 420

Query: 421 EKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFAALDV 480
           E++PSP GES +DC  LS +P VSFTIGGKVF+L PE+YVLK+ +GPV +CISGF ALD+
Sbjct: 421 ERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDI 480

Query: 481 PPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAES 514
           PPPRGPLWILGDVFMG YHTVFD+GN +VGFAE+
Sbjct: 481 PPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

BLAST of Sed0026314 vs. TAIR 10
Match: AT4G04460.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 633.3 bits (1632), Expect = 1.8e-181
Identity = 307/519 (59.15%), Postives = 397/519 (76.49%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSAS----NDGKVRIGLKRRNFDQNNRVAGKIATKESV 60
           MGT  + F  V  +  L   L+ +AS     DG +RIGLK+R  D++NR+A ++  K   
Sbjct: 1   MGTRFQSFLLVFLLSCLI--LISTASCERNGDGTIRIGLKKRKLDRSNRLASQLFLKNRG 60

Query: 61  SLKGSEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           S    + Y     L D  + D+V LKNYL+AQY+G+I IGTPPQKFTV+FDTGSSNLW+P
Sbjct: 61  SHWSPKHY---FRLNDE-NADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIP 120

Query: 121 SSKC-LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQD 180
           S+KC LSVAC  HSKY++  SS+Y+KNGK ASI+YGTGAISGYFS D VKVG++VV++Q+
Sbjct: 121 STKCYLSVACYFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQE 180

Query: 181 FIEATREPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVD 240
           FIEAT EP +TF+LA+FDGI GLGFKEISVG++ PVWYNMV++ LVKE +FSFW NRN  
Sbjct: 181 FIEATSEPGITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRN-- 240

Query: 241 ADEEQGGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIAD 300
             + +GGEIVFGGVDP H+KGEHT+VPVT KGYWQFDMGD+ I G  T +C+ GCSAIAD
Sbjct: 241 PKDPEGGEIVFGGVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIAD 300

Query: 301 SGTSLLAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGL 360
           SGTSLL GP+T+IT INHAIGA G+VS ECK VV +YG+T++  LLA++ PKK+CS +G+
Sbjct: 301 SGTSLLTGPSTVITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLAQEDPKKVCSQIGV 360

Query: 361 CTFDGTRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYM 420
           C +DGT+ VSMGI+SVVD+ T   S  L   +C+ CEMA VW +S+L   QTQ++IL Y 
Sbjct: 361 CAYDGTQSVSMGIQSVVDDGT---SGLLNQAMCSACEMAAVWMESELTQNQTQERILAYA 420

Query: 421 DRLCEKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFA 480
             LC+ +P+   +S +DC  +S++P V+F+IGG+ F+L P+ Y+ K+ +G  ++C SGF 
Sbjct: 421 AELCDHIPTQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFT 480

Query: 481 ALDVPPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           A+D+ PPRGPLWILGD+FMGPYHTVFDYG  RVGFA++A
Sbjct: 481 AMDIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 508

BLAST of Sed0026314 vs. TAIR 10
Match: AT4G04460.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 621.7 bits (1602), Expect = 5.3e-178
Identity = 305/519 (58.77%), Postives = 394/519 (75.92%), Query Frame = 0

Query: 1   MGTGLKLFTAVLFICFLTFPLVFSAS----NDGKVRIGLKRRNFDQNNRVAGKIATKESV 60
           MGT  + F  V  +  L   L+ +AS     DG +RIGLK+R  D++NR+A ++  K   
Sbjct: 1   MGTRFQSFLLVFLLSCLI--LISTASCERNGDGTIRIGLKKRKLDRSNRLASQLFLKNRG 60

Query: 61  SLKGSEKYQASGNLGDSADTDIVALKNYLNAQYFGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           S    + Y     L D  + D+V LKNYL+AQY+G+I IGTPPQKFTV+FDTGSSNLW+P
Sbjct: 61  SHWSPKHY---FRLNDE-NADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIP 120

Query: 121 SSKC-LSVACLLHSKYRSRHSSTYKKNGKRASIKYGTGAISGYFSQDHVKVGNLVVQKQD 180
           S+KC LSVAC  HSKY++  SS+Y+KNGK ASI+YGTGAISGYFS D VKVG++VV++Q+
Sbjct: 121 STKCYLSVACYFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQE 180

Query: 181 FIEATREPSLTFVLAQFDGIFGLGFKEISVGDAVPVWYNMVDQNLVKELVFSFWFNRNVD 240
           FIEAT EP +TF+LA+FDGI GLGFKEISVG++ PVWYNMV++ LVKE +FSFW NRN  
Sbjct: 181 FIEATSEPGITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRN-- 240

Query: 241 ADEEQGGEIVFGGVDPYHYKGEHTYVPVTKKGYWQFDMGDVLINGSTTEFCSNGCSAIAD 300
             + +GGEIVFGGVDP H+KGEHT+VPVT KGYWQFDMGD+ I G  T +C+ GCSAIAD
Sbjct: 241 PKDPEGGEIVFGGVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIAD 300

Query: 301 SGTSLLAGPTTIITQINHAIGASGVVSEECKTVVAEYGETIIKMLLAKDQPKKICSTLGL 360
           SGTSLL GP+T+IT INHAIGA G+VS ECK VV +YG+T++  LLA    +K+CS +G+
Sbjct: 301 SGTSLLTGPSTVITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLA----QKVCSQIGV 360

Query: 361 CTFDGTRGVSMGIKSVVDNTTQKSSNGLRDILCNTCEMAVVWAQSQLKDEQTQDQILNYM 420
           C +DGT+ VSMGI+SVVD+ T   S  L   +C+ CEMA VW +S+L   QTQ++IL Y 
Sbjct: 361 CAYDGTQSVSMGIQSVVDDGT---SGLLNQAMCSACEMAAVWMESELTQNQTQERILAYA 420

Query: 421 DRLCEKLPSPMGESVIDCDSLSTLPNVSFTIGGKVFELKPEQYVLKVTQGPVTECISGFA 480
             LC+ +P+   +S +DC  +S++P V+F+IGG+ F+L P+ Y+ K+ +G  ++C SGF 
Sbjct: 421 AELCDHIPTQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFT 480

Query: 481 ALDVPPPRGPLWILGDVFMGPYHTVFDYGNLRVGFAESA 515
           A+D+ PPRGPLWILGD+FMGPYHTVFDYG  RVGFA++A
Sbjct: 481 AMDIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023553350.11.4e-26889.69aspartic proteinase A1-like [Cucurbita pepo subsp. pepo][more]
KAG7011412.12.3e-26889.69Aspartic proteinase A1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_004151126.13.9e-26889.32aspartic proteinase A1 [Cucumis sativus] >XP_011650512.1 aspartic proteinase A1 ... [more]
XP_022932233.15.2e-26889.49aspartic proteinase A1-like [Cucurbita moschata][more]
XP_023007789.18.8e-26889.30aspartic proteinase A1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O040578.1e-20867.39Aspartic proteinase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
O653901.4e-20769.25Aspartic proteinase A1 OS=Arabidopsis thaliana OX=3702 GN=APA1 PE=1 SV=1[more]
Q8VYL33.1e-19963.62Aspartic proteinase A2 OS=Arabidopsis thaliana OX=3702 GN=APA2 PE=2 SV=1[more]
Q424561.2e-19865.04Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g... [more]
P422102.9e-19765.05Phytepsin OS=Hordeum vulgare OX=4513 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F1372.5e-26889.49aspartic proteinase A1-like OS=Cucurbita moschata OX=3662 GN=LOC111438595 PE=3 S... [more]
A0A6J1L5Y54.3e-26889.30aspartic proteinase A1-like OS=Cucurbita maxima OX=3661 GN=LOC111500293 PE=3 SV=... [more]
A0A5D3DKC05.2e-26688.80Aspartic proteinase A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A6J1DCK43.5e-26286.43aspartic proteinase A1-like OS=Momordica charantia OX=3673 GN=LOC111019253 PE=3 ... [more]
A0A0A0L2W74.0e-24282.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G066750 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G11910.19.9e-20969.25aspartic proteinase A1 [more]
AT1G62290.12.2e-20063.62Saposin-like aspartyl protease family protein [more]
AT1G62290.22.2e-20063.62Saposin-like aspartyl protease family protein [more]
AT4G04460.11.8e-18159.15Saposin-like aspartyl protease family protein [more]
AT4G04460.25.3e-17858.77Saposin-like aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 487..502
score: 58.48
coord: 292..303
score: 58.07
coord: 242..255
score: 46.31
coord: 95..115
score: 72.91
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47966BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATEDcoord: 8..514
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 31..248
e-value: 2.1E-74
score: 251.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 249..514
e-value: 9.7E-121
score: 403.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 11..512
NoneNo IPR availableGENE3D1.10.225.10coord: 320..423
e-value: 9.7E-121
score: 403.5
NoneNo IPR availablePANTHERPTHR47966:SF39ASPARTIC PROTEINASE A1-LIKEcoord: 8..514
IPR007856Saposin-like type B, region 1PFAMPF05184SapB_1coord: 387..423
e-value: 7.9E-13
score: 48.2
IPR008138Saposin B type, region 2PFAMPF03489SapB_2coord: 324..356
e-value: 4.0E-12
score: 46.1
IPR033121Peptidase family A1 domainPFAMPF00026Aspcoord: 88..512
e-value: 1.9E-132
score: 441.8
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 89..511
score: 72.008598
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 104..115
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 292..303
IPR008139Saposin B type domainPROSITEPS50015SAP_Bcoord: 384..425
score: 11.982399
IPR008139Saposin B type domainPROSITEPS50015SAP_Bcoord: 320..360
score: 12.7624
IPR033869PhytepsinCDDcd06098phytepsincoord: 79..512
e-value: 0.0
score: 616.307
IPR011001Saposin-likeSUPERFAMILY47862Saposincoord: 321..423

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0026314.1Sed0026314.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity