Sed0025910 (gene) Chayote v1

Overview
NameSed0025910
Typegene
OrganismSechium edule (Chayote v1)
DescriptionRNase H domain-containing protein
LocationLG12: 24475600 .. 24476256 (-)
RNA-Seq ExpressionSed0025910
SyntenySed0025910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTAATAATAATGAAACGATCAATTTGCTATTTGATTGGTCCTACTGCGTTAATTATATTCAAAAGTTTCGAGAAGCAAATAGTTCTACCATTCTACGCTCGACAAAGCTTTGTACAAATAATGTAAAAAGAGAAATTTTTTGGATTCCTCCAACTAATAACTCTTATAAACTAAATTGTGATGCATCAATTAGAAAGGATTTCTTGGCAGGTGGTGTTGGAGCTATTTTACGAAATAATAAAAGGGAATCGATGCTGACCTTGGCGACACCCTTTCAAGGGATAACATACCCTCTAGTGCTTGAAGCAATAACTGTTCGAGAAGGCCTTTTGATGGTGGCTGACATGGGATTTCAACACCTGGTGGTGGAATCAGACTCGGCTGGATTAATCGCAGGCTTAAATAAGCAACTTCTGAATCGTTCAGAATTAGGTAGAATCCTTGAAGAAATTGCAGGTCTCACAAGGAAGTATTCTTCTGTTACTTTTCAATGGTGCAATCGAAAGGCCAGCGACGTTGCTTATGCACTGGCGAACTTGGCGATTATACTTCAAGATGAAATGTTTTGGATGGAGGAGGTGCCGATATCTGTGGCACCTTTGTATTGCTATGAAAAGATGGCATGTGGCCATTCTATTTCTTTGTCCTTTTAA

mRNA sequence

ATGAGTAATAATAATGAAACGATCAATTTGCTATTTGATTGGTCCTACTGCGTTAATTATATTCAAAAGTTTCGAGAAGCAAATAGTTCTACCATTCTACGCTCGACAAAGCTTTGTACAAATAATGTAAAAAGAGAAATTTTTTGGATTCCTCCAACTAATAACTCTTATAAACTAAATTGTGATGCATCAATTAGAAAGGATTTCTTGGCAGGTGGTGTTGGAGCTATTTTACGAAATAATAAAAGGGAATCGATGCTGACCTTGGCGACACCCTTTCAAGGGATAACATACCCTCTAGTGCTTGAAGCAATAACTGTTCGAGAAGGCCTTTTGATGGTGGCTGACATGGGATTTCAACACCTGGTGGTGGAATCAGACTCGGCTGGATTAATCGCAGGCTTAAATAAGCAACTTCTGAATCGTTCAGAATTAGGTAGAATCCTTGAAGAAATTGCAGGTCTCACAAGGAAGTATTCTTCTGTTACTTTTCAATGGTGCAATCGAAAGGCCAGCGACGTTGCTTATGCACTGGCGAACTTGGCGATTATACTTCAAGATGAAATGTTTTGGATGGAGGAGGTGCCGATATCTGTGGCACCTTTGTATTGCTATGAAAAGATGGCATGTGGCCATTCTATTTCTTTGTCCTTTTAA

Coding sequence (CDS)

ATGAGTAATAATAATGAAACGATCAATTTGCTATTTGATTGGTCCTACTGCGTTAATTATATTCAAAAGTTTCGAGAAGCAAATAGTTCTACCATTCTACGCTCGACAAAGCTTTGTACAAATAATGTAAAAAGAGAAATTTTTTGGATTCCTCCAACTAATAACTCTTATAAACTAAATTGTGATGCATCAATTAGAAAGGATTTCTTGGCAGGTGGTGTTGGAGCTATTTTACGAAATAATAAAAGGGAATCGATGCTGACCTTGGCGACACCCTTTCAAGGGATAACATACCCTCTAGTGCTTGAAGCAATAACTGTTCGAGAAGGCCTTTTGATGGTGGCTGACATGGGATTTCAACACCTGGTGGTGGAATCAGACTCGGCTGGATTAATCGCAGGCTTAAATAAGCAACTTCTGAATCGTTCAGAATTAGGTAGAATCCTTGAAGAAATTGCAGGTCTCACAAGGAAGTATTCTTCTGTTACTTTTCAATGGTGCAATCGAAAGGCCAGCGACGTTGCTTATGCACTGGCGAACTTGGCGATTATACTTCAAGATGAAATGTTTTGGATGGAGGAGGTGCCGATATCTGTGGCACCTTTGTATTGCTATGAAAAGATGGCATGTGGCCATTCTATTTCTTTGTCCTTTTAA

Protein sequence

MSNNNETINLLFDWSYCVNYIQKFREANSSTILRSTKLCTNNVKREIFWIPPTNNSYKLNCDASIRKDFLAGGVGAILRNNKRESMLTLATPFQGITYPLVLEAITVREGLLMVADMGFQHLVVESDSAGLIAGLNKQLLNRSELGRILEEIAGLTRKYSSVTFQWCNRKASDVAYALANLAIILQDEMFWMEEVPISVAPLYCYEKMACGHSISLSF
Homology
BLAST of Sed0025910 vs. NCBI nr
Match: XP_021763631.1 (uncharacterized protein LOC110728264 [Chenopodium quinoa])

HSP 1 Score: 113.6 bits (283), Expect = 2.1e-21
Identity = 70/206 (33.98%), Postives = 111/206 (53.88%), Query Frame = 0

Query: 8   INLLFDWSYCVNYIQKFREAN----SSTILRSTKLCTNNVKREIFWIPPTNNSYKLNCDA 67
           ++ L   S  +N+  ++  AN    +++I RS+++          WIPPT++ YKLN DA
Sbjct: 89  VDPLIACSKTLNFFDEYVAANLREPTTSIERSSEVTG-------LWIPPTSSRYKLNTDA 148

Query: 68  SIRKDFLAGGVGAILRNNKRESMLTLATPFQGITYPLVLEAITVREGLLMVADMGFQHLV 127
           SI KD   GG+G ++R+N  + M+T      G      +EA+ +  G+  V D GF+ L 
Sbjct: 149 SISKD-RRGGLGMVVRDNIGDVMMTWGKSLNGTGIISEIEAVALLFGMRAVFDAGFRKLE 208

Query: 128 VESDSAGLIAGLNKQLLNRSELGRILEEIAGLTRKYSSVTFQWCNRKASDVAYALANLAI 187
           V SD   +I  LN      S    I+ +I      +  V F +C R  + VA+++ANL++
Sbjct: 209 VSSDCLQVIELLNGSKHENSPTQLIVTDILSFAYSFDFVCFSFCPRLCNKVAHSMANLSL 268

Query: 188 ILQDEMFWMEEVPISVAPLYCYEKMA 210
            L +E FWME+ P SV PL  ++K+A
Sbjct: 269 SLSEERFWMEDCPASVYPLVDFDKVA 286

BLAST of Sed0025910 vs. NCBI nr
Match: XP_030930636.1 (uncharacterized protein LOC115956377 [Quercus lobata])

HSP 1 Score: 103.6 bits (257), Expect = 2.2e-18
Identity = 53/158 (33.54%), Postives = 84/158 (53.16%), Query Frame = 0

Query: 49  WIPPTNNSYKLNCDASIRKDFLAGGVGAILRNNKRESMLTLATPFQGITYPLVLEAITVR 108
           W PP ++++KLN DA++  +    GVGA++RN K E M  ++     +    V E I  R
Sbjct: 37  WHPPPDSAFKLNFDAAVFSELSCSGVGAMIRNEKGEVMAAMSAKGPHVVDSAVAEVIACR 96

Query: 109 EGLLMVADMGFQHLVVESDSAGLIAGLNKQLLNRSELGRILEEIAGLTRKYSSVTFQWCN 168
             L    + GF  LVVE D+  ++  L     + S LG I+++I  LTR +  V F +  
Sbjct: 97  RALEFACEAGFTDLVVEGDNLSVMKSLAASKTDHSWLGHIIQDIKWLTRSFRKVLFSYVR 156

Query: 169 RKASDVAYALANLAIILQDEMFWMEEVPISVAPLYCYE 207
           R A+ VA+ LA  A  + ++M+W+E+ P  V     Y+
Sbjct: 157 RAANSVAHGLARYAKYVHEDMYWIEDNPPPVLEALYYD 194

BLAST of Sed0025910 vs. NCBI nr
Match: XP_023928118.1 (uncharacterized protein LOC112039474 [Quercus suber])

HSP 1 Score: 103.2 bits (256), Expect = 2.9e-18
Identity = 54/158 (34.18%), Postives = 82/158 (51.90%), Query Frame = 0

Query: 49  WIPPTNNSYKLNCDASIRKDFLAGGVGAILRNNKRESMLTLATPFQGITYPLVLEAITVR 108
           W PP ++++KLN DA +       GVGA++RN   E M T+      +   +V E I  R
Sbjct: 154 WRPPPDSTFKLNFDAVVFSKLSCSGVGAMIRNENGEVMATMLARVPHVVDSVVAEVIACR 213

Query: 109 EGLLMVADMGFQHLVVESDSAGLIAGLNKQLLNRSELGRILEEIAGLTRKYSSVTFQWCN 168
             +      GF  LVVE D+  ++  L     + S LG I+++I  LTR +  V+F +  
Sbjct: 214 RAMKFACKAGFTDLVVEGDNLSVMKSLTTSETDLSWLGHIIQDIKWLTRSFRRVSFSYVR 273

Query: 169 RKASDVAYALANLAIILQDEMFWMEEVPISVAPLYCYE 207
           R A+ VAY LA  A  + ++M+WME+ P  V     Y+
Sbjct: 274 RAANSVAYGLARYAKDIHEDMYWMEDNPPPVLEALYYD 311

BLAST of Sed0025910 vs. NCBI nr
Match: XP_030968337.1 (uncharacterized protein LOC115988853 [Quercus lobata])

HSP 1 Score: 102.8 bits (255), Expect = 3.7e-18
Identity = 59/178 (33.15%), Postives = 90/178 (50.56%), Query Frame = 0

Query: 19   NYIQKFREANSSTILRSTKLCTNNVKREIFWIPPTNNSYKLNCDASIRKDFLAGGVGAIL 78
            +++++FREA    + R        V RE+ W P   N +KLN DA+I  +  A G GA++
Sbjct: 999  DFLKEFREAQQHLVTR------RQVAREVQWRPSPRNGFKLNFDAAIFDELNATGFGAVI 1058

Query: 79   RNNKRESMLTLATPFQGITYPLVLEAITVREGLLMVADMGFQHLVVESDSAGLIAGLNKQ 138
            RN + E M  +A     ++     E +  R  L    D GF  L+VE D+  ++  + + 
Sbjct: 1059 RNGEGEVMAAMAAKGPPVSGSEEAEVLACRNALEFAVDAGFTELLVEGDNVSVMQRILRS 1118

Query: 139  LLNRSELGRILEEIAGLTRKYSSVTFQWCNRKASDVAYALANLAIILQDEMFWMEEVP 197
              N S LG + E+I  L      V+ +W +R  ++VAY LA  A  L DE  W+EE P
Sbjct: 1119 QPNGSWLGHLYEDIKCLCAGLCVVSVEWVSRTTNEVAYCLAKHARGLVDESVWLEESP 1170

BLAST of Sed0025910 vs. NCBI nr
Match: XP_023920779.1 (uncharacterized protein LOC112032247 [Quercus suber])

HSP 1 Score: 100.5 bits (249), Expect = 1.9e-17
Identity = 58/188 (30.85%), Postives = 96/188 (51.06%), Query Frame = 0

Query: 20  YIQKFREANSSTILRSTKLCTNNVKREIFWIPPTNNSYKLNCDASIRKDFLAGGVGAILR 79
           ++ +FR+A     LR    C      E  W PP+   +KLN DA++  +    G+GA++R
Sbjct: 82  FLDEFRQAQGQ--LRIHVSCPG----ESVWRPPSVFPFKLNFDAAVFSELSCSGIGAVIR 141

Query: 80  NNKRESMLTLATPFQGITYPLVLEAITVREGLLMVADMGFQHLVVESDSAGLIAGLNKQL 139
           N+  E M  ++     +    V E +  R  +    + GF  LVVE D+  ++  L    
Sbjct: 142 NDNGEVMAAMSARGPLVADSAVAEVLACRRAMEFAREAGFTDLVVEGDNLCVMKALAASE 201

Query: 140 LNRSELGRILEEIAGLTRKYSSVTFQWCNRKASDVAYALANLAIILQDEMFWMEE-VPIS 199
           L+RS LG I+++I  L R +  ++F +  R A+ VA+ LA  A  + ++M+WME+  P  
Sbjct: 202 LDRSWLGHIIQDIKWLARNFRRISFSYVRRAANTVAHGLARYAKYIHEDMYWMEDNHPPP 261

Query: 200 VAPLYCYE 207
           V    CY+
Sbjct: 262 VLEALCYD 263

BLAST of Sed0025910 vs. ExPASy TrEMBL
Match: A0A803L9N8 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 2.3e-21
Identity = 69/205 (33.66%), Postives = 110/205 (53.66%), Query Frame = 0

Query: 8   INLLFDWSYCVNYIQKFREAN----SSTILRSTKLCTNNVKREIFWIPPTNNSYKLNCDA 67
           ++ L   S  +N+  ++  AN    +++I RS+++          WIPPT++ YKLN DA
Sbjct: 89  VDPLIACSKTLNFFDEYVAANLREPTTSIERSSEVTG-------LWIPPTSSRYKLNTDA 148

Query: 68  SIRKDFLAGGVGAILRNNKRESMLTLATPFQGITYPLVLEAITVREGLLMVADMGFQHLV 127
           SI KD   GG+G ++R+N  + M+T      G      +EA+ +  G+  V D GF+ L 
Sbjct: 149 SISKD-RRGGLGMVVRDNIGDVMMTWGKSLNGTGIISEIEAVALLFGMRAVFDAGFRKLE 208

Query: 128 VESDSAGLIAGLNKQLLNRSELGRILEEIAGLTRKYSSVTFQWCNRKASDVAYALANLAI 187
           V SD   +I  LN      S    I+ +I      +  V F +C R  + VA+++ANL++
Sbjct: 209 VSSDCLQVIELLNGSKHENSPTQLIVTDILSFAYSFDFVCFSFCPRLCNKVAHSMANLSL 268

Query: 188 ILQDEMFWMEEVPISVAPLYCYEKM 209
            L +E FWME+ P SV PL  ++K+
Sbjct: 269 SLSEERFWMEDCPASVYPLVDFDKL 285

BLAST of Sed0025910 vs. ExPASy TrEMBL
Match: A0A7N2LST3 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 5.8e-17
Identity = 52/148 (35.14%), Postives = 79/148 (53.38%), Query Frame = 0

Query: 49  WIPPTNNSYKLNCDASIRKDFLAGGVGAILRNNKRESMLTLATPFQGITYPLVLEAITVR 108
           W PPT  SYK+N DA++  +  A G G ++RN + E M +LA     +      + +  R
Sbjct: 9   WEPPTGYSYKINVDAAVFAEINASGFGVVVRNERAEVMASLAAKGPPVQDSEEAKLLACR 68

Query: 109 EGLLMVADMGFQHLVVESDSAGLIAGLNKQLLNRSELGRILEEIAGLTRKYSSVTFQWCN 168
           + L    D GF  +V+E DS  ++  ++    NRS LG I E+I  L   + S +  +  
Sbjct: 69  KALEFAVDAGFMDVVLEGDSLNVMRAISTGKANRSRLGHIYEDIQCLAAGFRSYSVSFVK 128

Query: 169 RKASDVAYALANLAIILQDEMFWMEEVP 197
           R A+ VA+ALA  A  + DE+ W+EE P
Sbjct: 129 RSANGVAHALACFAKNVGDELVWLEESP 156

BLAST of Sed0025910 vs. ExPASy TrEMBL
Match: A0A5B6V4L7 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_008285 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.3e-16
Identity = 53/171 (30.99%), Postives = 88/171 (51.46%), Query Frame = 0

Query: 37  KLCTNNVKREIFWIPPTNNSYKLNCDASIRKDFLAGGVGAILRNNKRESMLTLATPFQGI 96
           +L T N      W PP N+  K+N DAS  +       G I RN +  +M     P++ I
Sbjct: 318 RLRTTNESTAPVWKPPANDIIKINFDASFNRLTRCSNSGIIARNKEGLTMAACTFPWENI 377

Query: 97  TYPLVLEAITVREGLLMVADMGFQHLVVESDSAGLIAGLNKQLLNRSELGRILEEIAGLT 156
             P++ EA    + ++M  +MGFQ + +E D+  +I  L     +RS +  +++EI G  
Sbjct: 378 PNPVMAEARACLQAIIMAEEMGFQDVCIEGDALSVIRKLKAAEEDRSCISNLIKEIKGKV 437

Query: 157 RKYSSVTFQWCNRKASDVAYALANLAIILQDEMFWMEEVPISVAPLYCYEK 208
             + SV+F++  R+A+ VA+A+A      +   +W+EE P +V  L   EK
Sbjct: 438 VNFRSVSFKYVPREANKVAHAMAQEGARYEQPRYWIEEAPHAVEVLVNQEK 488

BLAST of Sed0025910 vs. ExPASy TrEMBL
Match: A0A7J8YG77 (RNase H domain-containing protein OS=Gossypium aridum OX=34290 GN=Goari_000289 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.9e-16
Identity = 58/192 (30.21%), Postives = 95/192 (49.48%), Query Frame = 0

Query: 18  VNYIQKF-REANSSTILRSTKLCTNNVKREIFWIPPTNNSYKLNCDASIRKDFLAGGVGA 77
           VN+I+ + RE         T+L      RE+FW P   +  K N DA+  +  +    G 
Sbjct: 99  VNFIRAYLRELGQVKSSIKTRLS----PREVFWRPLKEDQVKANFDAAYSRQDMTATAGV 158

Query: 78  ILRNNKRESMLTLATPFQGITYPLVLEAITVREGLLMVADMGFQHLVVESDSAGLIAGLN 137
           I+RN++   M     P   I  P   EA      ++   +MGF+ LVVE DS  +I  L 
Sbjct: 159 IIRNHEGFVMGACPYPLGRIGDPTTTEAKACLHAVIFGEEMGFRDLVVEGDSLTVIKKLK 218

Query: 138 KQLLNRSELGRILEEIAGLTRKYSSVTFQWCNRKASDVAYALANLAIILQDEMFWMEEVP 197
              ++RS +G I+ EI      + +++F++  RK S+ A+ALA     L +  +W+EEVP
Sbjct: 219 SDSVDRSVIGNIISEIQRKKLSFGTLSFEYTPRKTSEEAHALATREYNLDNPSYWIEEVP 278

Query: 198 ISVAPLYCYEKM 209
             + P+   +++
Sbjct: 279 TKIEPIIANDQI 286

BLAST of Sed0025910 vs. ExPASy TrEMBL
Match: A0A2P5EYY2 (Ribonuclease H-like domain containing protein OS=Trema orientale OX=63057 GN=TorRG33x02_133770 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.9e-16
Identity = 55/186 (29.57%), Postives = 95/186 (51.08%), Query Frame = 0

Query: 14  WSYCVNYIQKFREANSSTILRSTKLCTNNVKREIFWIPPTNNSYKLNCDASIRKDFLAGG 73
           +++C+NY+++F+ A      R++   T +  R I W PP+ + YKLN DA+I +     G
Sbjct: 4   FTWCLNYLREFQTAQQQ---RASVSSTQSAPRPIKWQPPSTHGYKLNVDAAISESQRCVG 63

Query: 74  VGAILRNNKRESMLTLATPFQGITYPLVLEAITVREGLLMVADMGFQHLVVESDSAGLIA 133
            GAI+R+     +  +A  F     P   E   +  G+     +      VE+D+  +++
Sbjct: 64  FGAIIRDTTGFVLAAVAKKFHNPLKPKTAEVAALSFGINWATKVFPSFEAVETDAQAVVS 123

Query: 134 GLNKQLLNRSELGRILEEIAGLTRKYSSVTFQWCNRKASDVAYALANLAIILQDEMFWME 193
            L      RSELG  L+++  L      V+  +  R+ ++  Y LA LA+ L DE+FWME
Sbjct: 124 ALKTAHSYRSELGCFLKDVTSLLTTPPGVSVSYVPRECNNTTYGLAKLALRL-DELFWME 183

Query: 194 EVPISV 200
           ++  S+
Sbjct: 184 KISFSI 185

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_021763631.12.1e-2133.98uncharacterized protein LOC110728264 [Chenopodium quinoa][more]
XP_030930636.12.2e-1833.54uncharacterized protein LOC115956377 [Quercus lobata][more]
XP_023928118.12.9e-1834.18uncharacterized protein LOC112039474 [Quercus suber][more]
XP_030968337.13.7e-1833.15uncharacterized protein LOC115988853 [Quercus lobata][more]
XP_023920779.11.9e-1730.85uncharacterized protein LOC112032247 [Quercus suber][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A803L9N82.3e-2133.66Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1[more]
A0A7N2LST35.8e-1735.14RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A5B6V4L71.3e-1630.99Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_008285 PE=4 SV=1[more]
A0A7J8YG772.9e-1630.21RNase H domain-containing protein OS=Gossypium aridum OX=34290 GN=Goari_000289 P... [more]
A0A2P5EYY22.9e-1629.57Ribonuclease H-like domain containing protein OS=Trema orientale OX=63057 GN=Tor... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 60..182
e-value: 5.6E-19
score: 68.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 55..186
e-value: 5.8E-14
score: 54.2
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 42..192
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 59..180
e-value: 3.06488E-17
score: 72.732
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 56..186

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0025910.1Sed0025910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity