Sed0024861 (gene) Chayote v1

BLAST of Sed0024861 vs. NCBI nr
Match: XP_022989707.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022989715.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 620/767 (80.83%), Postives = 693/767 (90.35%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           MG  ELW+LL  IMLA S AA+D+QTYIIHMD TKMATTNPEQWYTS+I S+NQLSS++D
Sbjct: 1   MGITELWLLLLSIMLATSAAAVDQQTYIIHMDATKMATTNPEQWYTSIIHSINQLSSIND 60

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           D+ EASNAAEILY+YKTA SGF+AKLST+KL+SLSK PGFLAATP+KLLQLHTTH+P+FL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQFL 120

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL++ HGLWN+SNLASD+IIGV+DTGIWPEHISFQDKGLPPVP KWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSRSN 180

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CNKKL+GA+ +IKGYE++LGRLN TGTFRSARDS GHGTHTASTAAGN+VYKASL+NQ M
Sbjct: 181 CNKKLVGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G ATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAV+DGVDVLS+SLGGGDGFFY++E A
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IA FGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTFP +VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIFEG 360

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSL+ G +  ++PLVYNKTAG G+EA++CT GSLVPS+VKGKIV+CERG NSR EKGE V
Sbjct: 361 SSLHSGNSIGELPLVYNKTAG-GEEASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQV 420

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           KLAGG GMILINTQLEGEELF D HVLPA  LG S GKA++NYIASSKH   ASI F GT
Sbjct: 421 KLAGGVGMILINTQLEGEELFGDSHVLPAINLGASAGKAIINYIASSKHPPKASILFEGT 480

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
           +YGSRAPR+AAFSSRGP+FF PYVIKPDITAPGVNILAAWP +VSP+ELKSDKRRVLFNI
Sbjct: 481 RYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNI 540

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 600
           ISGTSMSCPH+SGIAAL+KSAHKNWSPAAIKSALMTTAYV DNK+SLISDVG  S   AD
Sbjct: 541 ISGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGPAD 600

Query: 601 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSVK 660
           P+AFGSGHV+PE+AVDPGLVYDI P+DYLNYLCSL YTSKQVGLVSRGNF+CPSKR  ++
Sbjct: 601 PYAFGSGHVDPEKAVDPGLVYDIVPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTVLQ 660

Query: 661 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFG 720
           PGDLNYPSFSV MK KAKN  V FKRTVTNVG P SDYTVKINNP GI ++VKP KLSF 
Sbjct: 661 PGDLNYPSFSVSMKNKAKN--VRFKRTVTNVGTPSSDYTVKINNPSGIRVSVKPEKLSFR 720

Query: 721 KLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
           + G+KLSY+VSFVAL + E   +FSFGS+VW+SGKY+VRSPIAV W+
Sbjct: 721 RSGQKLSYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Sed0024861 vs. NCBI nr
Match: KAG6599889.1 (Subtilisin-like protease 1.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1249.6 bits (3232), Expect = 0.0e+00
Identity = 613/767 (79.92%), Postives = 690/767 (89.96%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           MG  ELW+LLSI++  ++ AA D+Q+YIIHMD TKMATTNP+QWYTS+ID++NQLSS++D
Sbjct: 1   MGITELWLLLSIMLATSAAAAADQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIND 60

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           D+ EASNAAEILY+YKTA SGF+AKLST+KL+SLSK PGFL+ATP+KLLQLHTTH+P+FL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKFL 120

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL++ HGLWN+SNLASD+IIGV+DTGIWPEHISFQDKGLP VP KWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRSN 180

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CNKKLIGA  +IKGYE++LGRLN TGTFRSARDS GHGTHTASTAAGN+VYKASL+NQ M
Sbjct: 181 CNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G ATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAV+DGVDVLS+SLGGGDGFFY++E A
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IA FGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTF  +VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFEG 360

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSL+ G +  Q+PLVYNKTAG G+EAN+CT GSLVPS+VKGKIV+CERG NSR EKGE V
Sbjct: 361 SSLHSGNSIGQLPLVYNKTAG-GEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQV 420

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           KLAGG GMILINTQLEGEELFAD HVLPA  LG S GKA++NYIASSK    ASI F GT
Sbjct: 421 KLAGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAIINYIASSKQPPKASILFEGT 480

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
           +YGSRAPR+AAFSSRGP+FF PYVIKPDITAPGVNILAAWP +VSP+ELKSDKRRVLFNI
Sbjct: 481 RYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNI 540

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 600
           ISGTSMSCPH+SGIAAL+KSAH NWSPAAIKSALMTTAYV DNK+S+ISDVG  S   AD
Sbjct: 541 ISGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPAD 600

Query: 601 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSVK 660
           P+AFGSGHV+PE+AVDPGLVYDI P+DYLNYLCSL YTSKQVGLVSRGNF+CPSKR  V+
Sbjct: 601 PYAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRNLVQ 660

Query: 661 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFG 720
           PGDLNYPSFSV MK +AKN  VTFKRTVTNVG P SDYTVKINNP GI ++VKP KLSF 
Sbjct: 661 PGDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSFR 720

Query: 721 KLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
           + G+KL Y+VSFVAL + E   +FSFGS+VW+SGKY+VRSPIAV W+
Sbjct: 721 RSGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Sed0024861 vs. NCBI nr
Match: XP_022943077.1 (subtilisin-like protease SBT1.1 [Cucurbita moschata] >XP_022943079.1 subtilisin-like protease SBT1.1 [Cucurbita moschata])

HSP 1 Score: 1247.6 bits (3227), Expect = 0.0e+00
Identity = 612/767 (79.79%), Postives = 689/767 (89.83%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           MG  ELW+LLSI++  ++ AA D+Q+YIIHMD TKMATTNP+QWYTS+ID++NQLSS++D
Sbjct: 1   MGITELWLLLSIMLATSAAAAADQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIND 60

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           D+ EASNAAEILY+YKTA SGF+AKLST+KL+SLSK PGFL+ATP+KLLQLHTTH+P+FL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKFL 120

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL++ HGLWN+SNLASD+IIGV+DTGIWPEHISFQDKGLP VP KWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRSN 180

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CNKKLIGA  +IKGYE++LGRLN TGTFRSARDS GHGTHTASTAAGN+VYKASL+NQ M
Sbjct: 181 CNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G ATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAV+DGVDVLS+SLGGGDGFFY++E A
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IA FGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTF  +VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFEG 360

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSL+ G +  Q+PLVYNKTAG G+EAN+CT GSLVPS+VKGKIV+CERG NSR EKGE V
Sbjct: 361 SSLHSGNSIGQLPLVYNKTAG-GEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQV 420

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           KLAGG GMILINTQLEGEELFAD HVLPA  LG S GKA++NYIASSK    ASI F GT
Sbjct: 421 KLAGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAIINYIASSKQPPKASILFEGT 480

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
           +YGSRAPR+AAFSSRGP+FF PYVIKPDITAPGVNILAAWP +VSP+ELKSDKRRVLFNI
Sbjct: 481 RYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNI 540

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 600
           ISGTSMSCPH+SGIAAL+KSAH NWSPAAIKSALMTTAYV DNK+S+ISDVG  S   AD
Sbjct: 541 ISGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPAD 600

Query: 601 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSVK 660
           P+AFGSGHV+PE+AVDPGLVYDI P+DYLNYLCSL YTSKQVGLVSRGNF+CPS R  V+
Sbjct: 601 PYAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSNRNLVQ 660

Query: 661 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFG 720
           PGDLNYPSFSV MK +AKN  VTFKRTVTNVG P SDYTVKINNP GI ++VKP KLSF 
Sbjct: 661 PGDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSFR 720

Query: 721 KLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
           + G+KL Y+VSFVAL + E   +FSFGS+VW+SGKY+VRSPIAV W+
Sbjct: 721 RSGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Sed0024861 vs. NCBI nr
Match: XP_023544752.1 (subtilisin-like protease SBT1.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1242.6 bits (3214), Expect = 0.0e+00
Identity = 615/767 (80.18%), Postives = 690/767 (89.96%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           MG  ELW+LLS IMLA S AA+D+Q+YIIHMDTTKMATTNP+QWYTS+ID++NQLSS++D
Sbjct: 1   MGITELWLLLS-IMLATSAAAVDQQSYIIHMDTTKMATTNPQQWYTSIIDTVNQLSSIND 60

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           D+ EASNAAEILY+YKTA SGF+AKLST+KL SLSK PGFL+ATP+KLLQLHTTH+P+FL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLLSLSKLPGFLSATPDKLLQLHTTHTPKFL 120

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL++ HGLWN+S LASD+IIGV+DTGIWPEHISFQDKGL PVP KWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASYLASDIIIGVIDTGIWPEHISFQDKGLSPVPKKWKGTCQAGPKFSRSN 180

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CN KLIGA+ +IKGYE++LGRLN TGTFRSARDS GHGTHTASTAAGN+VYKASL+NQ M
Sbjct: 181 CNNKLIGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G ATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAV+DGVDVLS+SLGGGDGFFY++E A
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IA FGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTF  +VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFEG 360

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSL+ G +  Q+PLVYNKTAG G+EAN+CT GSLVPS+VKGKIV+CERG NSR EKGE V
Sbjct: 361 SSLHSGNSIGQLPLVYNKTAG-GEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQV 420

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           KLAGG GMILINTQLEGEELFAD HVLPA  LG S GKA+ NYIASSK    ASI F GT
Sbjct: 421 KLAGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAITNYIASSKQPPKASILFEGT 480

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
           +YGSRAPR+AAFSSRGP+FF PYVIKPDITAPGVNILAAWP +VSP+ELKSD+RRVLFNI
Sbjct: 481 RYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDERRVLFNI 540

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 600
           ISGTSMSCPH+SGIAAL+KSAHKNWSPAAIKSALMTTAYVTDNK+S+ISDVG  S   AD
Sbjct: 541 ISGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVTDNKRSVISDVGRPSGGPAD 600

Query: 601 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSVK 660
           P+AFGSGHV+PE+AVDPGLVYDIAP+DYLNYLCSL YTSKQVGLVSRGNF+CPSK   ++
Sbjct: 601 PYAFGSGHVDPEKAVDPGLVYDIAPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKGTVLQ 660

Query: 661 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFG 720
           PGDLNYPSFSV MK +AKN  VT KRTVTNVG P SDYTVKINNP GI ++VKP KLSF 
Sbjct: 661 PGDLNYPSFSVSMKNRAKN--VTLKRTVTNVGTPTSDYTVKINNPSGIAVSVKPKKLSFR 720

Query: 721 KLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
           + G+KLSY+VSFVA  + E   +FSFGS+VW+SGKY+VRSPIAV W+
Sbjct: 721 RSGQKLSYQVSFVASGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 763

BLAST of Sed0024861 vs. NCBI nr
Match: XP_038893069.1 (subtilisin-like protease SBT1.1 [Benincasa hispida] >XP_038893070.1 subtilisin-like protease SBT1.1 [Benincasa hispida])

HSP 1 Score: 1239.6 bits (3206), Expect = 0.0e+00
Identity = 617/768 (80.34%), Postives = 682/768 (88.80%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           MGFRE+W+ LS IMLA + AA+D+QTYIIHMDTTKM TTNPEQWYT+MIDSLN+L SLDD
Sbjct: 1   MGFREVWMFLS-IMLAIATAAVDQQTYIIHMDTTKMTTTNPEQWYTAMIDSLNELPSLDD 60

Query: 61  D-EEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEF 120
           + +EEAS+ AEILYVYKTA SGFAAKLS KKL+SLSK PGFLAATPN+LLQLHTTHSP+F
Sbjct: 61  ENKEEASDTAEILYVYKTALSGFAAKLSRKKLDSLSKIPGFLAATPNELLQLHTTHSPQF 120

Query: 121 LGLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRS 180
           LGL++DHGLWNSSNLASD+IIG+LDTGIWPEHISFQDKGL PVP+KWKGICQAGPKFS S
Sbjct: 121 LGLERDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPSKWKGICQAGPKFSPS 180

Query: 181 NCNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQA 240
           NCNKKLIGA  +IKGYE+++G LN TGTFRS RDS GHGTHTASTAAG++V KAS FNQ 
Sbjct: 181 NCNKKLIGASAYIKGYEAIVGTLNETGTFRSPRDSDGHGTHTASTAAGSMVNKASFFNQG 240

Query: 241 MGEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEET 300
           MG ATGM +TSRIAAYKVCWP GCA+ DILAA+D AV DGVDVLS+SLGGG G FY++  
Sbjct: 241 MGVATGMMYTSRIAAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNI 300

Query: 301 AIATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFE 360
           AIA FGA++NGVFVSCSAGNSGP+ISTV N APWIMTVAASYTDRTFPTTVKLGNG+VFE
Sbjct: 301 AIAAFGAIQNGVFVSCSAGNSGPFISTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFE 360

Query: 361 GSSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEV 420
           GSSLYYGKN N +PLVYN TAG G E N CT GSL P++VKGKIV+CERG NSRT+KGE 
Sbjct: 361 GSSLYYGKNINYLPLVYNNTAGDGQETNFCTAGSLDPTMVKGKIVLCERGSNSRTDKGEQ 420

Query: 421 VKLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGG 480
           VKLAGGAGMILINTQ EGEELFAD HVLPATTLG S GKA+L+YIASSK Q+ AS+AF G
Sbjct: 421 VKLAGGAGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKSQAKASMAFEG 480

Query: 481 TKYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFN 540
           TKYGSRAPRVAAFSSRGP+F  P V+KPD+TAPGVNILAAWP IVSP+EL SDKRRVLFN
Sbjct: 481 TKYGSRAPRVAAFSSRGPSFVGPDVMKPDVTAPGVNILAAWPPIVSPSELDSDKRRVLFN 540

Query: 541 IISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNA 600
           IISGTSMSCPH+SG+AAL+KSAHK+WSPAAIKSALMTTAYVTDNK +L+SDVG  S   A
Sbjct: 541 IISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKMNLVSDVGHPSGGPA 600

Query: 601 DPFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSV 660
           DPFAFGSGHV+PE+A DPGLVYDI P+DY+NYLCSLKY S Q+ LVSRGNFTC SKR  +
Sbjct: 601 DPFAFGSGHVDPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTLL 660

Query: 661 KPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSF 720
           +P DLNYPSFSVFMKKKAKNVS+T KRTVTNVGIP SDYTVKINNPKGI ITVKP KLSF
Sbjct: 661 QPRDLNYPSFSVFMKKKAKNVSITSKRTVTNVGIPRSDYTVKINNPKGIRITVKPEKLSF 720

Query: 721 GKLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
           G LGEKLS++VSFVAL   E   +FSFG +VWLSGKY VRSPIAVTWQ
Sbjct: 721 GSLGEKLSFQVSFVALGGKEALSKFSFGDLVWLSGKYAVRSPIAVTWQ 767

BLAST of Sed0024861 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 2.9e-241
Identity = 432/768 (56.25%), Postives = 557/768 (72.53%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           M F   +++   ++  ASN +  +QTY+IH       TT+ +   TS+ +SL Q  +++D
Sbjct: 18  MMFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLFNSL-QTENIND 77

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           D+    +  EI Y+Y+ A SGF+A L+  +L+++    GF++A P++LL LHTT+S EFL
Sbjct: 78  DD---FSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFL 137

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL+   GLWN ++L+SDVIIG++DTGI PEH+SF+D  + PVP++W+G C  G  FS S 
Sbjct: 138 GLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSE 197

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CNKK+IGA  F KGYES++G++N T  FRS RD+ GHGTHTASTAAG++V KA+ F QA 
Sbjct: 198 CNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAK 257

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G A+GMRFTSRIAAYK CW  GCASTD++AAIDRA++DGVDV+S+SLGG    FY +  A
Sbjct: 258 GLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIA 317

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IA FGA++  +FVSCSAGNSGP  STV+N APW+MTVAASYTDRTFP  V++GN +   G
Sbjct: 318 IAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVG 377

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSLY GK+   +PL +N+TAG    A  C   SL   LV+GKIVIC RG + RT KGE V
Sbjct: 378 SSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEV 437

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           K +GGA M+L++T+ EGEEL AD HVLPA +LGFSDGK LLNY+A + + +TAS+ F GT
Sbjct: 438 KRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAAN-ATASVRFRGT 497

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
            YG+ AP VAAFSSRGP+   P + KPDI APG+NILA W    SP+ L+SD RRV FNI
Sbjct: 498 AYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNI 557

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKN-A 600
           ISGTSM+CPHISGIAALIKS H +WSPA IKSA+MTTA +TDN+   I D G++  ++ A
Sbjct: 558 ISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAA 617

Query: 601 DPFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSV 660
             FAFG+G+V+P RAVDPGLVYD +  DYLNYLCSL YTS+++ L S  N+TC S    +
Sbjct: 618 TAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVL 677

Query: 661 KPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSF 720
            PGDLNYPSF+V +   A   +V +KRTVTNVG P  +Y V +  PKG+++ V+P  L F
Sbjct: 678 SPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKF 737

Query: 721 GKLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
            K  E+LSY V++ A   S +    SFG +VW+  KYNVRSPIAVTW+
Sbjct: 738 QKARERLSYTVTYDA-EASRNSSSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Sed0024861 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 2.1e-196
Identity = 375/776 (48.32%), Postives = 502/776 (64.69%), Query Frame = 0

Query: 6   LWVLLSI--IMLAASNAA--LDEQTYIIHMDTTKMAT--TNPEQWYTSMIDSLNQLSSLD 65
           L+++LSI  I L A        ++TY+IHMD + M    TN  QWY+S I+S+ Q  S  
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS-- 71

Query: 66  DDEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEF 125
             +EE  N   ILY Y+TAF G AA+L+ ++   L +  G +A  P    +LHTT SP F
Sbjct: 72  --QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTF 131

Query: 126 LGLKKDHG--LWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFS 185
           LGL++     +W       DV++GVLDTGIWPE  SF D G+ PVP  W+G C+ G +F 
Sbjct: 132 LGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFL 191

Query: 186 RSNCNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFN 245
           + NCN+K++GA+VF +GYE+  G+++    ++S RD  GHGTHTA+T AG+ V  A+LF 
Sbjct: 192 KRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 251

Query: 246 QAMGEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKE 305
            A G A GM   +R+AAYKVCW  GC S+DIL+A+D+AV DGV VLSISLGGG   + ++
Sbjct: 252 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 311

Query: 306 ETAIATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEV 365
             +IATFGA+  GVFVSCSAGN GP   ++TNV+PWI TV AS  DR FP TVK+G    
Sbjct: 312 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 371

Query: 366 FEGSSLYYGK----NTNQIPLVY-NKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNS 425
           F+G SLY G+       Q PLVY  + A + D  + C  G+L    V GKIVIC+RG+  
Sbjct: 372 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 431

Query: 426 RTEKGEVVKLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQST 485
           R +KG+VVK AGG GM+L NT   GEEL AD H+LPA  +G  +GK +  Y  +SK ++T
Sbjct: 432 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KAT 491

Query: 486 ASIAFGGTKYGSR-APRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKS 545
           AS+   GT+ G + +P VAAFSSRGP F    ++KPD+ APGVNILAAW   ++P+ L S
Sbjct: 492 ASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 551

Query: 546 DKRRVLFNIISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDV 605
           D RRV FNI+SGTSMSCPH+SG+AALIKS H +WSPAAIKSALMTTAYV DN    ++D 
Sbjct: 552 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD- 611

Query: 606 GSSSNKNADPFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSR-GNF 665
            +S    + P+  G+GH++P RA DPGLVYDI P++Y  +LC+   +  Q+ + ++  N 
Sbjct: 612 -ASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 671

Query: 666 TCPSKRRSVKPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEI 725
           TC     +  PG+LNYP+ S    +     ++T +RTVTNVG  +S Y V ++  KG  +
Sbjct: 672 TC-KHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASV 731

Query: 726 TVKPMKLSFGKLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTW 767
           TV+P  L+F    +KLSY V+F      +      FG +VW S  + VRSP+ +TW
Sbjct: 732 TVQPKTLNFTSKHQKLSYTVTFRTRFRMK---RPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Sed0024861 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 2.1e-196
Identity = 378/784 (48.21%), Postives = 497/784 (63.39%), Query Frame = 0

Query: 3   FRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPE--QWYTSMIDSLNQLSSLDD 62
           F   + LL++   ++S ++ +  TYI+H+D     +  P    WYTS + SL        
Sbjct: 4   FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL-------- 63

Query: 63  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 122
                S+   I++ Y T F GF+A+L+++  + L  +P  ++  P ++  LHTT SPEFL
Sbjct: 64  ----TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFL 123

Query: 123 GLKKDH--GLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSR 182
           GL+     GL   S+  SD++IGV+DTG+WPE  SF D+GL PVP KWKG C A   F  
Sbjct: 124 GLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPE 183

Query: 183 SNCNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQ 242
           S CN+KL+GA+ F  GYE+  G++N T  FRS RDS GHGTHTAS +AG  V+ AS    
Sbjct: 184 SACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGY 243

Query: 243 AMGEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEE 302
           A G A GM   +R+AAYKVCW  GC  +DILAA D AV DGVDV+S+S+GG    +Y + 
Sbjct: 244 AHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDA 303

Query: 303 TAIATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVF 362
            AI  FGA+  G+FVS SAGN GP   TVTNVAPW+ TV A   DR FP  VKLGNG++ 
Sbjct: 304 IAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMI 363

Query: 363 EGSSLYYGKNTN---QIPLVYNKT--AGTGDEANLCTPGSLVPSLVKGKIVICERGLNSR 422
            G S+Y G   +     PLVY  +   G G  ++LC  GSL P+LVKGKIV+C+RG+NSR
Sbjct: 364 SGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSR 423

Query: 423 TEKGEVVKLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIA------SS 482
             KGE+V+  GG GMI+ N   +GE L AD HVLPAT++G S G  +  YI+      SS
Sbjct: 424 ATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSS 483

Query: 483 KHQSTASIAFGGTKYGSR-APRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSP 542
           KH  TA+I F GT+ G R AP VA+FS+RGP    P ++KPD+ APG+NILAAWP  + P
Sbjct: 484 KH-PTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGP 543

Query: 543 TELKSDKRRVLFNIISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKS 602
           + + SD RR  FNI+SGTSM+CPH+SG+AAL+K+AH +WSPAAI+SAL+TTAY  DN   
Sbjct: 544 SGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGE 603

Query: 603 LISDVGSSSNKNADPFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVS 662
            + D   S+   +    +GSGHV+P +A+DPGLVYDI   DY+N+LC+  YT   +  ++
Sbjct: 604 PMMD--ESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTIT 663

Query: 663 RGNFTCPSKRRSVKPGDLNYPSFS-VFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNP 722
           R    C   RR+   G+LNYPSFS VF +     +S  F RTVTNVG   S Y +KI  P
Sbjct: 664 RRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPP 723

Query: 723 KGIEITVKPMKLSFGKLGEKLSY--KVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIA 768
           +G  +TV+P KLSF ++G+KLS+  +V    +  S        G IVW  GK NV SP+ 
Sbjct: 724 RGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLV 772

BLAST of Sed0024861 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 3.7e-196
Identity = 373/768 (48.57%), Postives = 504/768 (65.62%), Query Frame = 0

Query: 7   WVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNP--EQWYTSMIDSLNQLSSLDDDEEE 66
           ++LL +     S+++ D+ TYI+HM  ++M ++      WY S + S+            
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI------------ 71

Query: 67  ASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFLGLKK 126
            S++AE+LY Y+ A  GF+ +L+ ++ +SL   PG ++  P    +LHTT +P FLGL +
Sbjct: 72  -SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE 131

Query: 127 DHG-LWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSNCNK 186
               L+  +   SDV++GVLDTG+WPE  S+ D+G  P+P+ WKG C+AG  F+ S CN+
Sbjct: 132 HTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNR 191

Query: 187 KLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAMGEA 246
           KLIGA+ F +GYES +G ++ +   RS RD  GHGTHT+STAAG+VV  ASL   A G A
Sbjct: 192 KLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTA 251

Query: 247 TGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETAIAT 306
            GM   +R+A YKVCW  GC S+DILAAID+A+ D V+VLS+SLGGG   +Y++  AI  
Sbjct: 252 RGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGA 311

Query: 307 FGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEGSSL 366
           F A+  G+ VSCSAGN+GP  S+++NVAPWI TV A   DR FP    LGNG+ F G SL
Sbjct: 312 FAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSL 371

Query: 367 YYGK--NTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVVK 426
           + G+      +P +Y   A      NLC  G+L+P  VKGKIV+C+RG+N+R +KG+VVK
Sbjct: 372 FKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVK 431

Query: 427 LAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGTK 486
            AGG GMIL NT   GEEL AD H+LPATT+G   G  + +Y+ +  +  TASI+  GT 
Sbjct: 432 AAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPN-PTASISILGTV 491

Query: 487 YGSR-APRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 546
            G + +P VAAFSSRGP    P ++KPD+ APGVNILAAW     PT L SD RRV FNI
Sbjct: 492 VGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNI 551

Query: 547 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 606
           ISGTSMSCPH+SG+AAL+KS H  WSPAAI+SALMTTAY T      + D+  ++ K + 
Sbjct: 552 ISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPST 611

Query: 607 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTC-PSKRRSV 666
           PF  G+GHV+P  A +PGL+YD+  EDYL +LC+L YTS Q+  VSR N+TC PSK  SV
Sbjct: 612 PFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSV 671

Query: 667 KPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKI-NNPKGIEITVKPMKLS 726
              DLNYPSF+V +       +  + RTVT+VG     Y+VK+ +   G++I+V+P  L+
Sbjct: 672 --ADLNYPSFAVNVDGVG---AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLN 731

Query: 727 FGKLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTW 767
           F +  EK SY V+F  ++ S+  G  SFGSI W  GK+ V SP+A++W
Sbjct: 732 FKEANEKKSYTVTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Sed0024861 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 5.1e-190
Identity = 369/752 (49.07%), Postives = 474/752 (63.03%), Query Frame = 0

Query: 24  EQTYII---HMDTTKMATTNPEQWYTSMIDSLNQLSSLDDDEEEASNAAEILYVYKTAFS 83
           ++TYII   H D  +   T+   WYTS ++S                 + +LY Y T+F 
Sbjct: 27  KKTYIIRVNHSDKPESFLTH-HDWYTSQLNS----------------ESSLLYTYTTSFH 86

Query: 84  GFAAKLSTKKLNS-LSKNPGFLAATPNKLLQLHTTHSPEFLGLKKDHGLWNSSNLASDVI 143
           GF+A L + + +S LS +   L    + L  LHTT +PEFLGL  + G+ +  + ++ VI
Sbjct: 87  GFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVI 146

Query: 144 IGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSNCNKKLIGAQVFIKGYESVL 203
           IGVLDTG+WPE  SF D  +P +P+KWKG C++G  F    CNKKLIGA+ F KG++   
Sbjct: 147 IGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMAS 206

Query: 204 -GRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAMGEATGMRFTSRIAAYKVC 263
            G  ++     S RD  GHGTHT++TAAG+ V  AS    A G A GM   +R+A YKVC
Sbjct: 207 GGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVC 266

Query: 264 WPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETAIATFGAVRNGVFVSCSAG 323
           W  GC  +DILAA+DRA++DGVDVLS+SLGGG   +Y++  AI  F A+  GVFVSCSAG
Sbjct: 267 WSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAG 326

Query: 324 NSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEGSSLY--YGKNTNQIPLVY 383
           NSGP  ++V NVAPW+MTV A   DR FP    LGNG+   G SLY   G  T  + LVY
Sbjct: 327 NSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY 386

Query: 384 NKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVVKLAGGAGMILINTQLE 443
           NK  G    +NLC PGSL  S+V+GKIV+C+RG+N+R EKG VV+ AGG GMI+ NT   
Sbjct: 387 NK--GNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAAS 446

Query: 444 GEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGTKYGSR-APRVAAFSSR 503
           GEEL AD H+LPA  +G   G  L  Y+ S   + TA + F GT    + +P VAAFSSR
Sbjct: 447 GEELVADSHLLPAIAVGKKTGDLLREYVKSDS-KPTALLVFKGTVLDVKPSPVVAAFSSR 506

Query: 504 GPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNIISGTSMSCPHISGIA 563
           GP    P ++KPD+  PGVNILA W   + PT L  D RR  FNI+SGTSMSCPHISG+A
Sbjct: 507 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 566

Query: 564 ALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNADPFAFGSGHVNPERAV 623
            L+K+AH  WSP+AIKSALMTTAYV DN  + + D   +S  N  P+A GSGHV+P++A+
Sbjct: 567 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSN--PYAHGSGHVDPQKAL 626

Query: 624 DPGLVYDIAPEDYLNYLCSLKYTSKQ-VGLVSRGNFTCPSKRRSVKPGDLNYPSFSVFMK 683
            PGLVYDI+ E+Y+ +LCSL YT    V +V R +  C SK+ S  PG LNYPSFSV   
Sbjct: 627 SPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNC-SKKFS-DPGQLNYPSFSVLFG 686

Query: 684 KKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFGKLGEKLSYKVSFVA 743
            K     V + R VTNVG   S Y V +N    + I+VKP KLSF  +GEK  Y V+FV+
Sbjct: 687 GKR---VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 746

Query: 744 LNESEDFGEFSFGSIVWLSGKYNVRSPIAVTW 767
                   +  FGSI W + ++ VRSP+A +W
Sbjct: 747 KKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of Sed0024861 vs. ExPASy TrEMBL
Match: A0A6J1JN56 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111486706 PE=3 SV=1)

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 620/767 (80.83%), Postives = 693/767 (90.35%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           MG  ELW+LL  IMLA S AA+D+QTYIIHMD TKMATTNPEQWYTS+I S+NQLSS++D
Sbjct: 1   MGITELWLLLLSIMLATSAAAVDQQTYIIHMDATKMATTNPEQWYTSIIHSINQLSSIND 60

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           D+ EASNAAEILY+YKTA SGF+AKLST+KL+SLSK PGFLAATP+KLLQLHTTH+P+FL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQFL 120

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL++ HGLWN+SNLASD+IIGV+DTGIWPEHISFQDKGLPPVP KWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSRSN 180

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CNKKL+GA+ +IKGYE++LGRLN TGTFRSARDS GHGTHTASTAAGN+VYKASL+NQ M
Sbjct: 181 CNKKLVGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G ATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAV+DGVDVLS+SLGGGDGFFY++E A
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IA FGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTFP +VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIFEG 360

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSL+ G +  ++PLVYNKTAG G+EA++CT GSLVPS+VKGKIV+CERG NSR EKGE V
Sbjct: 361 SSLHSGNSIGELPLVYNKTAG-GEEASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQV 420

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           KLAGG GMILINTQLEGEELF D HVLPA  LG S GKA++NYIASSKH   ASI F GT
Sbjct: 421 KLAGGVGMILINTQLEGEELFGDSHVLPAINLGASAGKAIINYIASSKHPPKASILFEGT 480

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
           +YGSRAPR+AAFSSRGP+FF PYVIKPDITAPGVNILAAWP +VSP+ELKSDKRRVLFNI
Sbjct: 481 RYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNI 540

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 600
           ISGTSMSCPH+SGIAAL+KSAHKNWSPAAIKSALMTTAYV DNK+SLISDVG  S   AD
Sbjct: 541 ISGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGPAD 600

Query: 601 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSVK 660
           P+AFGSGHV+PE+AVDPGLVYDI P+DYLNYLCSL YTSKQVGLVSRGNF+CPSKR  ++
Sbjct: 601 PYAFGSGHVDPEKAVDPGLVYDIVPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTVLQ 660

Query: 661 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFG 720
           PGDLNYPSFSV MK KAKN  V FKRTVTNVG P SDYTVKINNP GI ++VKP KLSF 
Sbjct: 661 PGDLNYPSFSVSMKNKAKN--VRFKRTVTNVGTPSSDYTVKINNPSGIRVSVKPEKLSFR 720

Query: 721 KLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
           + G+KLSY+VSFVAL + E   +FSFGS+VW+SGKY+VRSPIAV W+
Sbjct: 721 RSGQKLSYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Sed0024861 vs. ExPASy TrEMBL
Match: A0A6J1FT86 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111447921 PE=3 SV=1)

HSP 1 Score: 1247.6 bits (3227), Expect = 0.0e+00
Identity = 612/767 (79.79%), Postives = 689/767 (89.83%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           MG  ELW+LLSI++  ++ AA D+Q+YIIHMD TKMATTNP+QWYTS+ID++NQLSS++D
Sbjct: 1   MGITELWLLLSIMLATSAAAAADQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIND 60

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           D+ EASNAAEILY+YKTA SGF+AKLST+KL+SLSK PGFL+ATP+KLLQLHTTH+P+FL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKFL 120

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL++ HGLWN+SNLASD+IIGV+DTGIWPEHISFQDKGLP VP KWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRSN 180

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CNKKLIGA  +IKGYE++LGRLN TGTFRSARDS GHGTHTASTAAGN+VYKASL+NQ M
Sbjct: 181 CNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G ATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAV+DGVDVLS+SLGGGDGFFY++E A
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IA FGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTF  +VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFEG 360

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSL+ G +  Q+PLVYNKTAG G+EAN+CT GSLVPS+VKGKIV+CERG NSR EKGE V
Sbjct: 361 SSLHSGNSIGQLPLVYNKTAG-GEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQV 420

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           KLAGG GMILINTQLEGEELFAD HVLPA  LG S GKA++NYIASSK    ASI F GT
Sbjct: 421 KLAGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAIINYIASSKQPPKASILFEGT 480

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
           +YGSRAPR+AAFSSRGP+FF PYVIKPDITAPGVNILAAWP +VSP+ELKSDKRRVLFNI
Sbjct: 481 RYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNI 540

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 600
           ISGTSMSCPH+SGIAAL+KSAH NWSPAAIKSALMTTAYV DNK+S+ISDVG  S   AD
Sbjct: 541 ISGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPAD 600

Query: 601 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSVK 660
           P+AFGSGHV+PE+AVDPGLVYDI P+DYLNYLCSL YTSKQVGLVSRGNF+CPS R  V+
Sbjct: 601 PYAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSNRNLVQ 660

Query: 661 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFG 720
           PGDLNYPSFSV MK +AKN  VTFKRTVTNVG P SDYTVKINNP GI ++VKP KLSF 
Sbjct: 661 PGDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSFR 720

Query: 721 KLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
           + G+KL Y+VSFVAL + E   +FSFGS+VW+SGKY+VRSPIAV W+
Sbjct: 721 RSGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Sed0024861 vs. ExPASy TrEMBL
Match: A0A6J1JPC3 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111487676 PE=3 SV=1)

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 606/767 (79.01%), Postives = 678/767 (88.40%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           MGFRE+ + LS I LA S AA+D+Q+YIIHMDTTKMA  +PEQWYT++IDS+N++SSL D
Sbjct: 1   MGFREVCLFLS-IFLATSAAAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSL-D 60

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           D+EEAS+AA+ILYVYKTA SGFAAKLSTKKL+SLSK PGFLAATPN+LLQLHTTHSP+FL
Sbjct: 61  DQEEASSAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFL 120

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL+++HGLWNSSNLASD++IG+LDTGIWPEHISFQDKGLPPVP KWKG CQAG KFS SN
Sbjct: 121 GLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSN 180

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CN+KLIGA  +IKGYE+++GRLNATGTFRS RDS GHGTHTASTAAGN+V KAS FNQAM
Sbjct: 181 CNRKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAM 240

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G ATGMRFTSRIAAYKVCW EGCA+ DILAAIDRAV DGVDVLS+SLGG    FYK++ A
Sbjct: 241 GVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIA 300

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IATFGAVRNGVFVSCSAGNSGP  STV+N+APWIMTVAASYTDRTFP TVKLGNG+VFEG
Sbjct: 301 IATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEG 360

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSLY G +  Q+PLVYN TAG G+EAN+CT GSLVPSLVKGKIV+CERG NSRT KGE V
Sbjct: 361 SSLYSGNSIGQLPLVYNNTAG-GEEANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQV 420

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           KLAGGAGMILINTQLEGEELFAD HVLPA  LG S G+A++ YI+SSKHQ  A IAF GT
Sbjct: 421 KLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGT 480

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
           K+G+RAPRVAAFSSRGP+   P VIKPD+TAPGVNILAAWP I SP+EL+SDKRRVLFNI
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNI 540

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 600
           ISGTSMSCPH+SG+AAL+KSAHK+WSPAAIKSALMTTAY  DN+ S ISDVGS S K A+
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPAN 600

Query: 601 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSVK 660
           PFAFGSGHV+PE+A DPGL+YDI P+DYLNY CSL Y S Q+GLVSRGNFTCPSKRR  +
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 661 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFG 720
           PG+LNYPSFSVFMKKKAKNVSVT KRTVTNVG P SDYTVKI NPKGI I V+P KLSF 
Sbjct: 661 PGNLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGIGIRVEPEKLSFR 720

Query: 721 KLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
           + G+KLSY+VSFVAL + E  G FSFGS+VW+SGKY VRSPIAVTW+
Sbjct: 721 RYGQKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of Sed0024861 vs. ExPASy TrEMBL
Match: A0A6J1FMR4 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111446877 PE=3 SV=1)

HSP 1 Score: 1204.1 bits (3114), Expect = 0.0e+00
Identity = 600/767 (78.23%), Postives = 676/767 (88.14%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           MGFRE+ + LS I LA S AA+D+Q+YIIHMDTTKMA   PEQWYT++IDS+N++SSL +
Sbjct: 1   MGFREVCLFLS-IFLATSTAAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSL-E 60

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           D+EEASNAA+ILYVYKTA SGFAAKLSTKKL+SLSK PGFLAATPN+LLQLHTTHSP+FL
Sbjct: 61  DQEEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFL 120

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL+++HGLWNSSNLASD++IG+LDTGIWPEHISFQDKGLPPVP KWKG CQAG KFS SN
Sbjct: 121 GLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSN 180

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CN+KLIGA  +IKGYE+++GRLNATGTFRS RDS GHGTHTASTAAGN+V KAS FNQAM
Sbjct: 181 CNRKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAM 240

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G ATGMRFTSRIAAYKVCW EGCA+ DILAAIDRAV DGVDVLS+SLGG    FYK++ A
Sbjct: 241 GVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIA 300

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IATFGAVRNGVFVSCSAGNSGP  STV+N+APWIMTVAASYTDR+FP TVKLGNG+VFEG
Sbjct: 301 IATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFEG 360

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSLY G N  Q+PLVYN TAG G++AN+CT GSLVPS+VKGKIV+CERG NSRT KGE V
Sbjct: 361 SSLYSGNNIGQLPLVYNNTAG-GEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQV 420

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           KLAGGAGMILINTQLEGEELFAD HVLPA  LG S G+A++ YI+SSKHQ  A IAF GT
Sbjct: 421 KLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGT 480

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
           K+G+RAPRVAAFSSRGP+   P VIKPD+TAPGVNILAAWP I SP+E++SDKRRVLFN+
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNV 540

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 600
           ISGTSMSCPH+SG+AAL+KSAHK+WSPAAIKSALMTTAY  DN+ S ISDVGS+S K A+
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPAN 600

Query: 601 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSVK 660
           PFAFGSGHV+PE+A DPGL+YDI P+DYLNYLCSL Y S Q+GLVSRGNFTCPSKRR  +
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 661 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFG 720
            G LNYPSFSVFMKKKAKNVSVT KRTVTNVG P SDY+VKI NPKGI I+VKP KLSF 
Sbjct: 661 AGKLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSFR 720

Query: 721 KLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
           + G+KLSY+VSFVAL + E    FSFGS+VW+SG Y VRSPIAVTW+
Sbjct: 721 RYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of Sed0024861 vs. ExPASy TrEMBL
Match: A0A1S3C0J8 (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495084 PE=3 SV=1)

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 597/767 (77.84%), Postives = 665/767 (86.70%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           MGFRE+WVL   IMLA S+A +D+QTYIIHMDTTKM T NPEQWYT +IDS+N+LSSLDD
Sbjct: 1   MGFREVWVL--SIMLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDD 60

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           + EEASNAAEILYVYKTA SGFAAKL++KKL+SLSK PGFLAATPN+LLQLHTTHSP+FL
Sbjct: 61  N-EEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFL 120

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL++DHGLWN SNLASD+IIG+LDTGIWPEHISFQDKGL  VP KWKGICQ GP+FS SN
Sbjct: 121 GLQRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSN 180

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CNKKLIGA  +IKGYE+++GRLN TGTFRS RDS GHGTHTASTAAG++V  AS +NQ M
Sbjct: 181 CNKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGM 240

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G A+GMRFTSRI AYKVCWP GCA+ DILAA+D AV DGVDVLS+SLGGG   FYK+  A
Sbjct: 241 GVASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIA 300

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IA FGA++ GVFVSCSAGNSGP  STV N APWIMTVAASYTDRTFPTTVKLGNG+VFEG
Sbjct: 301 IAAFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEG 360

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSLYYGK+ N++PLVYN TAG G E N+C  GSL PS+VKGKIVICERG  SRTEKGE V
Sbjct: 361 SSLYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQV 420

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           KLAGG GMILINTQ EGEELFAD HVLPATTLG S GKA+L+YIASSK Q+ ASI F GT
Sbjct: 421 KLAGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGT 480

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
           KYGS+APRVAAFSSRGP+   P VIKPD+TAPGVNILAAWP IVSP+EL SD RRV+FNI
Sbjct: 481 KYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNI 540

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 600
           ISGTSMSCPH+SG+AAL+KSAH +WSPAAIKSALMTTAYVTD+K SLISDVG ++ + A 
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPAT 600

Query: 601 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSVK 660
           PF FGSGHV+PE+A DPGL+YDI P+DY+NYLCSLKY S Q+ LVSRGN TC SKR  VK
Sbjct: 601 PFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVK 660

Query: 661 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFG 720
           PGDLNYPSFSVFMKKKAK VS+T KRTVTNVGI  SDYTVKINNPKG+ + VKP KLSFG
Sbjct: 661 PGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFG 720

Query: 721 KLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
            LGE+LSYKVSFV+L   E   +FSFGS+VW+SGKY VRSPI VTWQ
Sbjct: 721 SLGEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of Sed0024861 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 836.3 bits (2159), Expect = 2.0e-242
Identity = 432/768 (56.25%), Postives = 557/768 (72.53%), Query Frame = 0

Query: 1   MGFRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPEQWYTSMIDSLNQLSSLDD 60
           M F   +++   ++  ASN +  +QTY+IH       TT+ +   TS+ +SL Q  +++D
Sbjct: 18  MMFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLFNSL-QTENIND 77

Query: 61  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 120
           D+    +  EI Y+Y+ A SGF+A L+  +L+++    GF++A P++LL LHTT+S EFL
Sbjct: 78  DD---FSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFL 137

Query: 121 GLKKDHGLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSN 180
           GL+   GLWN ++L+SDVIIG++DTGI PEH+SF+D  + PVP++W+G C  G  FS S 
Sbjct: 138 GLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSE 197

Query: 181 CNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAM 240
           CNKK+IGA  F KGYES++G++N T  FRS RD+ GHGTHTASTAAG++V KA+ F QA 
Sbjct: 198 CNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAK 257

Query: 241 GEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETA 300
           G A+GMRFTSRIAAYK CW  GCASTD++AAIDRA++DGVDV+S+SLGG    FY +  A
Sbjct: 258 GLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIA 317

Query: 301 IATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEG 360
           IA FGA++  +FVSCSAGNSGP  STV+N APW+MTVAASYTDRTFP  V++GN +   G
Sbjct: 318 IAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVG 377

Query: 361 SSLYYGKNTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVV 420
           SSLY GK+   +PL +N+TAG    A  C   SL   LV+GKIVIC RG + RT KGE V
Sbjct: 378 SSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEV 437

Query: 421 KLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGT 480
           K +GGA M+L++T+ EGEEL AD HVLPA +LGFSDGK LLNY+A + + +TAS+ F GT
Sbjct: 438 KRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAAN-ATASVRFRGT 497

Query: 481 KYGSRAPRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 540
            YG+ AP VAAFSSRGP+   P + KPDI APG+NILA W    SP+ L+SD RRV FNI
Sbjct: 498 AYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNI 557

Query: 541 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKN-A 600
           ISGTSM+CPHISGIAALIKS H +WSPA IKSA+MTTA +TDN+   I D G++  ++ A
Sbjct: 558 ISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAA 617

Query: 601 DPFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTCPSKRRSV 660
             FAFG+G+V+P RAVDPGLVYD +  DYLNYLCSL YTS+++ L S  N+TC S    +
Sbjct: 618 TAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVL 677

Query: 661 KPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSF 720
            PGDLNYPSF+V +   A   +V +KRTVTNVG P  +Y V +  PKG+++ V+P  L F
Sbjct: 678 SPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKF 737

Query: 721 GKLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTWQ 768
            K  E+LSY V++ A   S +    SFG +VW+  KYNVRSPIAVTW+
Sbjct: 738 QKARERLSYTVTYDA-EASRNSSSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Sed0024861 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 687.2 bits (1772), Expect = 1.5e-197
Identity = 378/784 (48.21%), Postives = 497/784 (63.39%), Query Frame = 0

Query: 3   FRELWVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNPE--QWYTSMIDSLNQLSSLDD 62
           F   + LL++   ++S ++ +  TYI+H+D     +  P    WYTS + SL        
Sbjct: 4   FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL-------- 63

Query: 63  DEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFL 122
                S+   I++ Y T F GF+A+L+++  + L  +P  ++  P ++  LHTT SPEFL
Sbjct: 64  ----TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFL 123

Query: 123 GLKKDH--GLWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSR 182
           GL+     GL   S+  SD++IGV+DTG+WPE  SF D+GL PVP KWKG C A   F  
Sbjct: 124 GLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPE 183

Query: 183 SNCNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQ 242
           S CN+KL+GA+ F  GYE+  G++N T  FRS RDS GHGTHTAS +AG  V+ AS    
Sbjct: 184 SACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGY 243

Query: 243 AMGEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEE 302
           A G A GM   +R+AAYKVCW  GC  +DILAA D AV DGVDV+S+S+GG    +Y + 
Sbjct: 244 AHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDA 303

Query: 303 TAIATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVF 362
            AI  FGA+  G+FVS SAGN GP   TVTNVAPW+ TV A   DR FP  VKLGNG++ 
Sbjct: 304 IAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMI 363

Query: 363 EGSSLYYGKNTN---QIPLVYNKT--AGTGDEANLCTPGSLVPSLVKGKIVICERGLNSR 422
            G S+Y G   +     PLVY  +   G G  ++LC  GSL P+LVKGKIV+C+RG+NSR
Sbjct: 364 SGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSR 423

Query: 423 TEKGEVVKLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIA------SS 482
             KGE+V+  GG GMI+ N   +GE L AD HVLPAT++G S G  +  YI+      SS
Sbjct: 424 ATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSS 483

Query: 483 KHQSTASIAFGGTKYGSR-APRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSP 542
           KH  TA+I F GT+ G R AP VA+FS+RGP    P ++KPD+ APG+NILAAWP  + P
Sbjct: 484 KH-PTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGP 543

Query: 543 TELKSDKRRVLFNIISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKS 602
           + + SD RR  FNI+SGTSM+CPH+SG+AAL+K+AH +WSPAAI+SAL+TTAY  DN   
Sbjct: 544 SGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGE 603

Query: 603 LISDVGSSSNKNADPFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVS 662
            + D   S+   +    +GSGHV+P +A+DPGLVYDI   DY+N+LC+  YT   +  ++
Sbjct: 604 PMMD--ESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTIT 663

Query: 663 RGNFTCPSKRRSVKPGDLNYPSFS-VFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNP 722
           R    C   RR+   G+LNYPSFS VF +     +S  F RTVTNVG   S Y +KI  P
Sbjct: 664 RRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPP 723

Query: 723 KGIEITVKPMKLSFGKLGEKLSY--KVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIA 768
           +G  +TV+P KLSF ++G+KLS+  +V    +  S        G IVW  GK NV SP+ 
Sbjct: 724 RGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLV 772

BLAST of Sed0024861 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 687.2 bits (1772), Expect = 1.5e-197
Identity = 375/776 (48.32%), Postives = 502/776 (64.69%), Query Frame = 0

Query: 6   LWVLLSI--IMLAASNAA--LDEQTYIIHMDTTKMAT--TNPEQWYTSMIDSLNQLSSLD 65
           L+++LSI  I L A        ++TY+IHMD + M    TN  QWY+S I+S+ Q  S  
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS-- 71

Query: 66  DDEEEASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEF 125
             +EE  N   ILY Y+TAF G AA+L+ ++   L +  G +A  P    +LHTT SP F
Sbjct: 72  --QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTF 131

Query: 126 LGLKKDHG--LWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFS 185
           LGL++     +W       DV++GVLDTGIWPE  SF D G+ PVP  W+G C+ G +F 
Sbjct: 132 LGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFL 191

Query: 186 RSNCNKKLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFN 245
           + NCN+K++GA+VF +GYE+  G+++    ++S RD  GHGTHTA+T AG+ V  A+LF 
Sbjct: 192 KRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 251

Query: 246 QAMGEATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKE 305
            A G A GM   +R+AAYKVCW  GC S+DIL+A+D+AV DGV VLSISLGGG   + ++
Sbjct: 252 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 311

Query: 306 ETAIATFGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEV 365
             +IATFGA+  GVFVSCSAGN GP   ++TNV+PWI TV AS  DR FP TVK+G    
Sbjct: 312 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 371

Query: 366 FEGSSLYYGK----NTNQIPLVY-NKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNS 425
           F+G SLY G+       Q PLVY  + A + D  + C  G+L    V GKIVIC+RG+  
Sbjct: 372 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 431

Query: 426 RTEKGEVVKLAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQST 485
           R +KG+VVK AGG GM+L NT   GEEL AD H+LPA  +G  +GK +  Y  +SK ++T
Sbjct: 432 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KAT 491

Query: 486 ASIAFGGTKYGSR-APRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKS 545
           AS+   GT+ G + +P VAAFSSRGP F    ++KPD+ APGVNILAAW   ++P+ L S
Sbjct: 492 ASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 551

Query: 546 DKRRVLFNIISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDV 605
           D RRV FNI+SGTSMSCPH+SG+AALIKS H +WSPAAIKSALMTTAYV DN    ++D 
Sbjct: 552 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD- 611

Query: 606 GSSSNKNADPFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSR-GNF 665
            +S    + P+  G+GH++P RA DPGLVYDI P++Y  +LC+   +  Q+ + ++  N 
Sbjct: 612 -ASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 671

Query: 666 TCPSKRRSVKPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEI 725
           TC     +  PG+LNYP+ S    +     ++T +RTVTNVG  +S Y V ++  KG  +
Sbjct: 672 TC-KHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASV 731

Query: 726 TVKPMKLSFGKLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTW 767
           TV+P  L+F    +KLSY V+F      +      FG +VW S  + VRSP+ +TW
Sbjct: 732 TVQPKTLNFTSKHQKLSYTVTFRTRFRMK---RPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Sed0024861 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 686.4 bits (1770), Expect = 2.6e-197
Identity = 373/768 (48.57%), Postives = 504/768 (65.62%), Query Frame = 0

Query: 7   WVLLSIIMLAASNAALDEQTYIIHMDTTKMATTNP--EQWYTSMIDSLNQLSSLDDDEEE 66
           ++LL +     S+++ D+ TYI+HM  ++M ++      WY S + S+            
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI------------ 71

Query: 67  ASNAAEILYVYKTAFSGFAAKLSTKKLNSLSKNPGFLAATPNKLLQLHTTHSPEFLGLKK 126
            S++AE+LY Y+ A  GF+ +L+ ++ +SL   PG ++  P    +LHTT +P FLGL +
Sbjct: 72  -SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE 131

Query: 127 DHG-LWNSSNLASDVIIGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSNCNK 186
               L+  +   SDV++GVLDTG+WPE  S+ D+G  P+P+ WKG C+AG  F+ S CN+
Sbjct: 132 HTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNR 191

Query: 187 KLIGAQVFIKGYESVLGRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAMGEA 246
           KLIGA+ F +GYES +G ++ +   RS RD  GHGTHT+STAAG+VV  ASL   A G A
Sbjct: 192 KLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTA 251

Query: 247 TGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETAIAT 306
            GM   +R+A YKVCW  GC S+DILAAID+A+ D V+VLS+SLGGG   +Y++  AI  
Sbjct: 252 RGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGA 311

Query: 307 FGAVRNGVFVSCSAGNSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEGSSL 366
           F A+  G+ VSCSAGN+GP  S+++NVAPWI TV A   DR FP    LGNG+ F G SL
Sbjct: 312 FAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSL 371

Query: 367 YYGK--NTNQIPLVYNKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVVK 426
           + G+      +P +Y   A      NLC  G+L+P  VKGKIV+C+RG+N+R +KG+VVK
Sbjct: 372 FKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVK 431

Query: 427 LAGGAGMILINTQLEGEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGTK 486
            AGG GMIL NT   GEEL AD H+LPATT+G   G  + +Y+ +  +  TASI+  GT 
Sbjct: 432 AAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPN-PTASISILGTV 491

Query: 487 YGSR-APRVAAFSSRGPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNI 546
            G + +P VAAFSSRGP    P ++KPD+ APGVNILAAW     PT L SD RRV FNI
Sbjct: 492 VGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNI 551

Query: 547 ISGTSMSCPHISGIAALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNAD 606
           ISGTSMSCPH+SG+AAL+KS H  WSPAAI+SALMTTAY T      + D+  ++ K + 
Sbjct: 552 ISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPST 611

Query: 607 PFAFGSGHVNPERAVDPGLVYDIAPEDYLNYLCSLKYTSKQVGLVSRGNFTC-PSKRRSV 666
           PF  G+GHV+P  A +PGL+YD+  EDYL +LC+L YTS Q+  VSR N+TC PSK  SV
Sbjct: 612 PFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSV 671

Query: 667 KPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKI-NNPKGIEITVKPMKLS 726
              DLNYPSF+V +       +  + RTVT+VG     Y+VK+ +   G++I+V+P  L+
Sbjct: 672 --ADLNYPSFAVNVDGVG---AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLN 731

Query: 727 FGKLGEKLSYKVSFVALNESEDFGEFSFGSIVWLSGKYNVRSPIAVTW 767
           F +  EK SY V+F  ++ S+  G  SFGSI W  GK+ V SP+A++W
Sbjct: 732 FKEANEKKSYTVTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Sed0024861 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 666.0 bits (1717), Expect = 3.6e-191
Identity = 369/752 (49.07%), Postives = 474/752 (63.03%), Query Frame = 0

Query: 24  EQTYII---HMDTTKMATTNPEQWYTSMIDSLNQLSSLDDDEEEASNAAEILYVYKTAFS 83
           ++TYII   H D  +   T+   WYTS ++S                 + +LY Y T+F 
Sbjct: 27  KKTYIIRVNHSDKPESFLTH-HDWYTSQLNS----------------ESSLLYTYTTSFH 86

Query: 84  GFAAKLSTKKLNS-LSKNPGFLAATPNKLLQLHTTHSPEFLGLKKDHGLWNSSNLASDVI 143
           GF+A L + + +S LS +   L    + L  LHTT +PEFLGL  + G+ +  + ++ VI
Sbjct: 87  GFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVI 146

Query: 144 IGVLDTGIWPEHISFQDKGLPPVPTKWKGICQAGPKFSRSNCNKKLIGAQVFIKGYESVL 203
           IGVLDTG+WPE  SF D  +P +P+KWKG C++G  F    CNKKLIGA+ F KG++   
Sbjct: 147 IGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMAS 206

Query: 204 -GRLNATGTFRSARDSHGHGTHTASTAAGNVVYKASLFNQAMGEATGMRFTSRIAAYKVC 263
            G  ++     S RD  GHGTHT++TAAG+ V  AS    A G A GM   +R+A YKVC
Sbjct: 207 GGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVC 266

Query: 264 WPEGCASTDILAAIDRAVMDGVDVLSISLGGGDGFFYKEETAIATFGAVRNGVFVSCSAG 323
           W  GC  +DILAA+DRA++DGVDVLS+SLGGG   +Y++  AI  F A+  GVFVSCSAG
Sbjct: 267 WSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAG 326

Query: 324 NSGPYISTVTNVAPWIMTVAASYTDRTFPTTVKLGNGEVFEGSSLY--YGKNTNQIPLVY 383
           NSGP  ++V NVAPW+MTV A   DR FP    LGNG+   G SLY   G  T  + LVY
Sbjct: 327 NSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY 386

Query: 384 NKTAGTGDEANLCTPGSLVPSLVKGKIVICERGLNSRTEKGEVVKLAGGAGMILINTQLE 443
           NK  G    +NLC PGSL  S+V+GKIV+C+RG+N+R EKG VV+ AGG GMI+ NT   
Sbjct: 387 NK--GNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAAS 446

Query: 444 GEELFADLHVLPATTLGFSDGKALLNYIASSKHQSTASIAFGGTKYGSR-APRVAAFSSR 503
           GEEL AD H+LPA  +G   G  L  Y+ S   + TA + F GT    + +P VAAFSSR
Sbjct: 447 GEELVADSHLLPAIAVGKKTGDLLREYVKSDS-KPTALLVFKGTVLDVKPSPVVAAFSSR 506

Query: 504 GPTFFHPYVIKPDITAPGVNILAAWPHIVSPTELKSDKRRVLFNIISGTSMSCPHISGIA 563
           GP    P ++KPD+  PGVNILA W   + PT L  D RR  FNI+SGTSMSCPHISG+A
Sbjct: 507 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 566

Query: 564 ALIKSAHKNWSPAAIKSALMTTAYVTDNKKSLISDVGSSSNKNADPFAFGSGHVNPERAV 623
            L+K+AH  WSP+AIKSALMTTAYV DN  + + D   +S  N  P+A GSGHV+P++A+
Sbjct: 567 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSN--PYAHGSGHVDPQKAL 626

Query: 624 DPGLVYDIAPEDYLNYLCSLKYTSKQ-VGLVSRGNFTCPSKRRSVKPGDLNYPSFSVFMK 683
            PGLVYDI+ E+Y+ +LCSL YT    V +V R +  C SK+ S  PG LNYPSFSV   
Sbjct: 627 SPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNC-SKKFS-DPGQLNYPSFSVLFG 686

Query: 684 KKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLSFGKLGEKLSYKVSFVA 743
            K     V + R VTNVG   S Y V +N    + I+VKP KLSF  +GEK  Y V+FV+
Sbjct: 687 GKR---VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 746

Query: 744 LNESEDFGEFSFGSIVWLSGKYNVRSPIAVTW 767
                   +  FGSI W + ++ VRSP+A +W
Sbjct: 747 KKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751