Sed0024414 (gene) Chayote v1

Overview
NameSed0024414
Typegene
OrganismSechium edule (Chayote v1)
Description(1->3)-beta-glucan endohydrolase
LocationLG12: 25418052 .. 25419769 (-)
RNA-Seq ExpressionSed0024414
SyntenySed0024414
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GACCAATTCAATTTTCCATTCTCTACAACTGCTTTCTTGGCTCTGTTTCTCATGATCCATCCATGAGGTACTTTTCCTTTCAGGCCTCGCCTATGGCTTCTTCCATCCTTTCCCTTCTCTTTCTTGGAGCTTTGATTTCTCCAGGTTTCATCCACACCATGGAGATGGAAATTGAAGATGTAGATCTGTCTCTTTTACCATTTGGATCTGCTTCTGCTATTCGAACCGGAAGCTTCCTTCGTCTTCCTCAATGCGCTGACCCTCGCCAAACAGGTAATCAAATAGTGGTTGTATGCTTAAATTTCAAAAACCAAAAATAAAAAACAAAATGGTTATCAAACAACACCTAAATTGTTATTTTATTGACAACTAAAGCTTCCACACGGCTCGATTATTGTCACTGGACATACCATAATCTTCACCCATAGAACTTTGCAAACTAAGAAGAGAATAACCATTATGGGTTGCATTAGTGGGAGTACTTAGAGGATATGGATTCAATCCATGGTATCATTTATCTAAAACTTAATTTCCTAGAGTTACTTTGACAACCGAATATACTAGGGTCAAGCTGTATCTCCGTGAGTGTTGTTAAACTGTATATAAGTTGACCCAAAAACTCAGCTGATATTAAAAAACTTGGAGAGAATAACTTAAATCAGAGTTTACATCCAGAACTAAATGATTTTTTGCTGCTTGAATATGTGTTTTCTGCCTTGCGAACTAATCAACATTACTTTTATATGAAAATTCATATGGCTGCAATACCAGAAGCCATTGGTGTTTGCTATTCAAGCTTTGGCAACAATTTACCTGATCCCAGAGAAGTAGTACAACTATACCAAAGCAACAACATTAACAAGATGAGGATCTATCATCCTAACATAGCTATATTAAATGCTCTTCGAGGATCGAACATCGAAGTCATTGTAGACATCCCGAATTCTGACCTCGAACATATGGTTAATCTTTCTTCTGCAACAAACTGGGTCAAAAGGAACATTTTAGCCTACGTGCCACACGTCAAATTTCGATACATTGCCATTGGGTACGTAGTGAAGCCATCCGATCCTGCTGCTCAGTACGTCTTACCAGCCATGAGCAGCTTTTACAGTGCAATTTCCGCAGCCAATTTGCAGGATCAAATAAAGGTTTCCACAGTAATATCCATCAGCTTGTTGTCGAACTCCTCATTTCCTCCATCTAATAGCTCATTTAGCTCAGAGGCTGATGGATTCATAGAACCAATTGTTAGCTTTCTAGTTAAGAATGGTTCACCACTTCTTGCTAATGTGTATCCCTACTTCATTTATACTGATATCACTCGAACGATCAGCCTCGATTATGCTTTGTTTATGCAATCTCCATTTCTTGCGGTACATGATGGAAATTTTGAGTACAACAGCCTTTTTGAATCGATGGTGGATGCTCTTTACGTAGCGCTTGAGAAGTCAGGTGGAGCCGGAGTGTCGATCGTGATATCGGAGAGCGGTTGGCCATCAGGTGGAGGCACGGCTGCTACACTCGAGAATGCAGGTATGTACTATAGAAATTTGATTAGCTTTGTTCGAGGTGGGACGCCGAGAAGGCCTAGACGAGCTATTGAAACGTATTTGTTTGCTATGTTTGATGAAAATTTGAAATCTCTTGAAATGGAAAAACATTTTGGTTTGTTCACATCTGATAAGAAACAAAAGTACCAACTTAGTTTTAGTTAA

mRNA sequence

GACCAATTCAATTTTCCATTCTCTACAACTGCTTTCTTGGCTCTGTTTCTCATGATCCATCCATGAGGTACTTTTCCTTTCAGGCCTCGCCTATGGCTTCTTCCATCCTTTCCCTTCTCTTTCTTGGAGCTTTGATTTCTCCAGGTTTCATCCACACCATGGAGATGGAAATTGAAGATGTAGATCTGTCTCTTTTACCATTTGGATCTGCTTCTGCTATTCGAACCGGAAGCTTCCTTCGTCTTCCTCAATGCGCTGACCCTCGCCAAACAGAAGCCATTGGTGTTTGCTATTCAAGCTTTGGCAACAATTTACCTGATCCCAGAGAAGTAGTACAACTATACCAAAGCAACAACATTAACAAGATGAGGATCTATCATCCTAACATAGCTATATTAAATGCTCTTCGAGGATCGAACATCGAAGTCATTGTAGACATCCCGAATTCTGACCTCGAACATATGGTTAATCTTTCTTCTGCAACAAACTGGGTCAAAAGGAACATTTTAGCCTACGTGCCACACGTCAAATTTCGATACATTGCCATTGGGTACGTAGTGAAGCCATCCGATCCTGCTGCTCAGTACGTCTTACCAGCCATGAGCAGCTTTTACAGTGCAATTTCCGCAGCCAATTTGCAGGATCAAATAAAGGTTTCCACAGTAATATCCATCAGCTTGTTGTCGAACTCCTCATTTCCTCCATCTAATAGCTCATTTAGCTCAGAGGCTGATGGATTCATAGAACCAATTGTTAGCTTTCTAGTTAAGAATGGTTCACCACTTCTTGCTAATGTGTATCCCTACTTCATTTATACTGATATCACTCGAACGATCAGCCTCGATTATGCTTTGTTTATGCAATCTCCATTTCTTGCGGTACATGATGGAAATTTTGAGTACAACAGCCTTTTTGAATCGATGGTGGATGCTCTTTACGTAGCGCTTGAGAAGTCAGGTGGAGCCGGAGTGTCGATCGTGATATCGGAGAGCGGTTGGCCATCAGGTGGAGGCACGGCTGCTACACTCGAGAATGCAGGTATGTACTATAGAAATTTGATTAGCTTTGTTCGAGGTGGGACGCCGAGAAGGCCTAGACGAGCTATTGAAACGTATTTGTTTGCTATGTTTGATGAAAATTTGAAATCTCTTGAAATGGAAAAACATTTTGGTTTGTTCACATCTGATAAGAAACAAAAGTACCAACTTAGTTTTAGTTAA

Coding sequence (CDS)

ATGAGGTACTTTTCCTTTCAGGCCTCGCCTATGGCTTCTTCCATCCTTTCCCTTCTCTTTCTTGGAGCTTTGATTTCTCCAGGTTTCATCCACACCATGGAGATGGAAATTGAAGATGTAGATCTGTCTCTTTTACCATTTGGATCTGCTTCTGCTATTCGAACCGGAAGCTTCCTTCGTCTTCCTCAATGCGCTGACCCTCGCCAAACAGAAGCCATTGGTGTTTGCTATTCAAGCTTTGGCAACAATTTACCTGATCCCAGAGAAGTAGTACAACTATACCAAAGCAACAACATTAACAAGATGAGGATCTATCATCCTAACATAGCTATATTAAATGCTCTTCGAGGATCGAACATCGAAGTCATTGTAGACATCCCGAATTCTGACCTCGAACATATGGTTAATCTTTCTTCTGCAACAAACTGGGTCAAAAGGAACATTTTAGCCTACGTGCCACACGTCAAATTTCGATACATTGCCATTGGGTACGTAGTGAAGCCATCCGATCCTGCTGCTCAGTACGTCTTACCAGCCATGAGCAGCTTTTACAGTGCAATTTCCGCAGCCAATTTGCAGGATCAAATAAAGGTTTCCACAGTAATATCCATCAGCTTGTTGTCGAACTCCTCATTTCCTCCATCTAATAGCTCATTTAGCTCAGAGGCTGATGGATTCATAGAACCAATTGTTAGCTTTCTAGTTAAGAATGGTTCACCACTTCTTGCTAATGTGTATCCCTACTTCATTTATACTGATATCACTCGAACGATCAGCCTCGATTATGCTTTGTTTATGCAATCTCCATTTCTTGCGGTACATGATGGAAATTTTGAGTACAACAGCCTTTTTGAATCGATGGTGGATGCTCTTTACGTAGCGCTTGAGAAGTCAGGTGGAGCCGGAGTGTCGATCGTGATATCGGAGAGCGGTTGGCCATCAGGTGGAGGCACGGCTGCTACACTCGAGAATGCAGGTATGTACTATAGAAATTTGATTAGCTTTGTTCGAGGTGGGACGCCGAGAAGGCCTAGACGAGCTATTGAAACGTATTTGTTTGCTATGTTTGATGAAAATTTGAAATCTCTTGAAATGGAAAAACATTTTGGTTTGTTCACATCTGATAAGAAACAAAAGTACCAACTTAGTTTTAGTTAA

Protein sequence

MRYFSFQASPMASSILSLLFLGALISPGFIHTMEMEIEDVDLSLLPFGSASAIRTGSFLRLPQCADPRQTEAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSDLEHMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYSAISAANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYFIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISESGWPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTSDKKQKYQLSFS
Homology
BLAST of Sed0024414 vs. NCBI nr
Match: XP_004139045.1 (glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KAE8653516.1 hypothetical protein Csa_006933 [Cucumis sativus])

HSP 1 Score: 559.7 bits (1441), Expect = 2.0e-155
Identity = 293/382 (76.70%), Postives = 321/382 (84.03%), Query Frame = 0

Query: 14  SILSLLFLGALISPGFIH---------TMEMEIEDVD-LSLLPFGSASAIRTGSFLRLPQ 73
           S + +L LG LISPGF            M M IED D ++LLPFG  SA   G F+RLPQ
Sbjct: 12  SFVLVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFG-GSASSVGRFIRLPQ 71

Query: 74  CADPRQTEAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVI 133
           C  PR+TE+IGVCYSS GNNLPD REVVQLY++N+I KMRIY+P+  ILNALRGSNIEVI
Sbjct: 72  CGGPRRTESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVI 131

Query: 134 VDIPNSDLEHMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSF 193
           V IPN+ +EH+VNLSSA NWV++NI AYVP+V FRYIA+G  VKP D  AQYVLPAMSS 
Sbjct: 132 VGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSI 191

Query: 194 YSAISAANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLA 253
           YSAISAANLQDQIKVSTVIS+S LSNSSFPPSN SFSSEA GF+EPI+ FL KNGSPLLA
Sbjct: 192 YSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLA 251

Query: 254 NVYPYFIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGV 313
           NVYPYF+YT+I +TISLDYALFMQSP L + DGNF+YNSLFESMVDALYVALEK GGA V
Sbjct: 252 NVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKV 311

Query: 314 SIVISESGWPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKSL 373
           SIVISESGWPSGGG  AT+ENAG YYRNLISFVR GTPRRPRRAIETYLFAMFDENLKSL
Sbjct: 312 SIVISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSL 371

Query: 374 EMEKHFGLFTSDKKQKYQLSFS 386
           EMEKHFGLFT DKK KYQLSFS
Sbjct: 372 EMEKHFGLFTPDKKSKYQLSFS 392

BLAST of Sed0024414 vs. NCBI nr
Match: XP_038880622.1 (glucan endo-1,3-beta-glucosidase-like [Benincasa hispida])

HSP 1 Score: 555.4 bits (1430), Expect = 3.7e-154
Identity = 291/370 (78.65%), Postives = 320/370 (86.49%), Query Frame = 0

Query: 18  LLFLGALISP-GFIHTMEMEIEDVD-LSLLPFGSASAIRTGSFLRLPQCADPRQTEAIGV 77
           +L LGA I P      M M IED D ++LLPFGSAS    GSF+RL QC  PR+TE+IGV
Sbjct: 16  VLLLGAFIPPESKSGNMVMAIEDEDSMALLPFGSASG--AGSFIRLSQCGGPRRTESIGV 75

Query: 78  CYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSDLEHMV 137
           CYSS GNNLPD +EVVQLY+SN+I KMRIY+P+ AILNALRGSNIE+ V IPN+D+EH+V
Sbjct: 76  CYSSAGNNLPDAKEVVQLYKSNDIKKMRIYNPDAAILNALRGSNIELTVGIPNTDIEHIV 135

Query: 138 NLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYSAISAANLQDQ 197
           NLSSA NWV++NILAYVP VKFRY+AIG  V+PSDP ++YVLPAMSS +SAISAANLQDQ
Sbjct: 136 NLSSAANWVEKNILAYVPLVKFRYVAIGNEVQPSDPISRYVLPAMSSIHSAISAANLQDQ 195

Query: 198 IKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYFIYTDIT 257
           IK+STVIS S LSNSSFPPSN SFSSEA GF+EPIV FLVKNGSPLLANVYPYF+YT+IT
Sbjct: 196 IKISTVISTSFLSNSSFPPSNGSFSSEAGGFMEPIVDFLVKNGSPLLANVYPYFVYTEIT 255

Query: 258 RTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISESGWPSG 317
           RTISLDYALFMQSP L + DGNF YNSLFESMVDALYVALEK GG  VSIVISESGWPSG
Sbjct: 256 RTISLDYALFMQSPSLVIRDGNFFYNSLFESMVDALYVALEKLGGDKVSIVISESGWPSG 315

Query: 318 GGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTSD 377
           GG  AT+ENAG YYRNLISFVR GTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFT D
Sbjct: 316 GGKGATVENAGTYYRNLISFVRTGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTPD 375

Query: 378 KKQKYQLSFS 386
           KK K+QLSFS
Sbjct: 376 KKSKFQLSFS 383

BLAST of Sed0024414 vs. NCBI nr
Match: KAG6590190.1 (Lichenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 548.1 bits (1411), Expect = 6.0e-152
Identity = 284/354 (80.23%), Postives = 312/354 (88.14%), Query Frame = 0

Query: 33  MEMEIEDVD-LSLLPFGSASAIRTGSFLRLPQCADPRQTEAIGVCYSSFGNNLPDPREVV 92
           M M IEDVD ++LLPFGSAS  R GSF RLPQC  PR+TE+IGVCY++ GNNLPD REVV
Sbjct: 13  MVMAIEDVDSMALLPFGSAS--RIGSFFRLPQCGGPRRTESIGVCYTNLGNNLPDAREVV 72

Query: 93  QLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSDLEHMVNLSSATNWVKRNILAY 152
           QLY+ + I KMRIY+P+ AILNALRG NIEVIV IPN+DLE +VNLSSA+NWV+RNI AY
Sbjct: 73  QLYKIHGIEKMRIYNPDTAILNALRGLNIEVIVGIPNTDLERIVNLSSASNWVQRNIQAY 132

Query: 153 VPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYSAISAANLQDQIKVSTVISISLLSNSS 212
           VPH+KFRY+A+G  V+PSD  A+YVLPA+SS  SAISAANLQDQIKVSTVISISLLSNSS
Sbjct: 133 VPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAANLQDQIKVSTVISISLLSNSS 192

Query: 213 FPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYFIYTDITRTISLDYALFMQSPFL 272
           FPPS SSFSSEA GFIEPIV FL KNGSPLLANVYPYF YTDI+RTISLDYALFMQSP L
Sbjct: 193 FPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFTYTDISRTISLDYALFMQSPTL 252

Query: 273 AVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISESGWPSGGGTAATLENAGMYYRN 332
            + DGNFEYNSLFESMVDALYVALEKSGGA VSIVI+ESGWPS G  AAT+ENAG YYRN
Sbjct: 253 VIRDGNFEYNSLFESMVDALYVALEKSGGAEVSIVIAESGWPSNGSAAATVENAGTYYRN 312

Query: 333 LISFVRGGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTSDKKQKYQLSFS 386
           L++FV  GTPRRPRRAIETYLFAMFDENLK +EMEKHFGLFT+DKK KYQLSFS
Sbjct: 313 LMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFGLFTADKKSKYQLSFS 364

BLAST of Sed0024414 vs. NCBI nr
Match: XP_022961205.1 (glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 548.1 bits (1411), Expect = 6.0e-152
Identity = 291/376 (77.39%), Postives = 321/376 (85.37%), Query Frame = 0

Query: 15  ILSLLFLGALISPGFI----HTMEMEIEDVD-LSLLPFGSASAIRTGSFLRLPQCADPRQ 74
           +L L  L +L+   FI      M M IEDVD ++LLPFGSAS  R GSF RLPQC  PR+
Sbjct: 7   VLLLASLISLVFHRFIGLKSGNMVMAIEDVDSMALLPFGSAS--RIGSFFRLPQCGGPRR 66

Query: 75  TEAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNS 134
           TE+IGVCY++ GNNLPD REVVQLY+ + I KMRIY+P+ AILNALRGSNIEVIV IPN+
Sbjct: 67  TESIGVCYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVGIPNT 126

Query: 135 DLEHMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYSAISA 194
           DLE +VNLSSA+NWV+RNI AYVPH+KFRY+A+G  V+PSD  A+YVLPA+SS  SAISA
Sbjct: 127 DLERIVNLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISA 186

Query: 195 ANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYF 254
           ANLQDQIKVSTVISISLLSNSSFPPS SSFSSEA GFIEPIV FL KNGSPLLANVYPYF
Sbjct: 187 ANLQDQIKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYF 246

Query: 255 IYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISE 314
            YTDITRTISLDYALFMQSP L + D NFEYNSLFESMVDALYVALEKSGGA VSIVI+E
Sbjct: 247 TYTDITRTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEVSIVIAE 306

Query: 315 SGWPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKSLEMEKHF 374
           SGWPS G  AAT+ENAG YYRNL++FV  GTPRRPRRAIETYLFAMFDENLK +EMEKHF
Sbjct: 307 SGWPSNGSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHF 366

Query: 375 GLFTSDKKQKYQLSFS 386
           GLFT+DKK KYQLSFS
Sbjct: 367 GLFTADKKSKYQLSFS 380

BLAST of Sed0024414 vs. NCBI nr
Match: KAG7023843.1 (Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 545.4 bits (1404), Expect = 3.9e-151
Identity = 283/353 (80.17%), Postives = 310/353 (87.82%), Query Frame = 0

Query: 33  MEMEIEDVD-LSLLPFGSASAIRTGSFLRLPQCADPRQTEAIGVCYSSFGNNLPDPREVV 92
           M M IEDVD ++LLPFGSAS  R GSF RLPQC  PR+TE+IGVCY++ GNNLPD REVV
Sbjct: 356 MVMAIEDVDSMALLPFGSAS--RIGSFFRLPQCGGPRRTESIGVCYTNLGNNLPDAREVV 415

Query: 93  QLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSDLEHMVNLSSATNWVKRNILAY 152
           QLY+ + I KMRIY+P+ AILNALRGSNIEVIV IPN+DLE +VNLSSA+NWV+RNI AY
Sbjct: 416 QLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVGIPNTDLERIVNLSSASNWVQRNIQAY 475

Query: 153 VPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYSAISAANLQDQIKVSTVISISLLSNSS 212
           VPH+KFRY+A+G  V+PSD  A+YVLPA+SS  SAISAANLQDQIKVSTVISISLLSNSS
Sbjct: 476 VPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAANLQDQIKVSTVISISLLSNSS 535

Query: 213 FPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYFIYTDITRTISLDYALFMQSPFL 272
           FPPS SSFSSEA GFIEPIV FL KNGSPLLANVYPYF YTDITRTISLDYALFMQSP L
Sbjct: 536 FPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFTYTDITRTISLDYALFMQSPTL 595

Query: 273 AVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISESGWPSGGGTAATLENAGMYYRN 332
            + D NFEYNSLFESMVDALYVALEKSGGA VSIVI+ESGWPS G  AAT+ENAG YYRN
Sbjct: 596 VIRDDNFEYNSLFESMVDALYVALEKSGGAEVSIVIAESGWPSNGSAAATVENAGTYYRN 655

Query: 333 LISFVRGGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTSDKKQKYQLSF 385
           L++FV  GTPRRPRRAIETYLFAMFDENLK +EMEKHFGLFT+DKK KYQL F
Sbjct: 656 LMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFGLFTADKKSKYQLIF 706

BLAST of Sed0024414 vs. ExPASy Swiss-Prot
Match: A7PQW3 (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)

HSP 1 Score: 350.5 bits (898), Expect = 2.4e-95
Identity = 185/320 (57.81%), Postives = 238/320 (74.38%), Query Frame = 0

Query: 71  EAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSD 130
           ++IGVCY   GNNLP   +V+ LY+SN I  MRIY PN   L ALRGS+IE+I+D+PN+D
Sbjct: 28  QSIGVCYGRNGNNLPSASQVINLYKSNGIGSMRIYDPNSDTLQALRGSDIELILDVPNTD 87

Query: 131 LEHMV-NLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSD---PAAQYVLPAMSSFYSA 190
           L+ +  + S+A  WV+ N++ Y   VKFRYIA+G  V P+      AQYVLPAM +  SA
Sbjct: 88  LQSLASDASAAATWVQNNVVNYASEVKFRYIAVGNEVLPTGSNAQYAQYVLPAMKNVQSA 147

Query: 191 ISAANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVY 250
           I++A LQDQIKVST  + S +   S+PPS  SFS +   FI PI+SFL +NGSPLLAN+Y
Sbjct: 148 ITSAGLQDQIKVSTA-TFSAVLGKSYPPSEGSFSDDVSSFINPIISFLAENGSPLLANIY 207

Query: 251 PYFIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIV 310
           PYF YT  T+ I LDYALF  S  + V DG+++Y +LF++++DALY ALEK+GG+ + IV
Sbjct: 208 PYFSYTGDTQNIRLDYALFTASG-VVVQDGSYQYQNLFDALLDALYAALEKAGGSNLKIV 267

Query: 311 ISESGWPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKS-LEM 370
           +SESGWPS GGTAAT++NA  YY+NLI+ V+GGTPR+   AIETYLFAMFDEN K+ LE 
Sbjct: 268 VSESGWPSEGGTAATVDNARTYYKNLINHVKGGTPRK-SGAIETYLFAMFDENQKTGLET 327

Query: 371 EKHFGLFTSDKKQKYQLSFS 386
           EKHFGLFT  ++ KYQ+SFS
Sbjct: 328 EKHFGLFTPGQESKYQISFS 344

BLAST of Sed0024414 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 335.1 bits (858), Expect = 1.0e-90
Identity = 173/322 (53.73%), Postives = 231/322 (71.74%), Query Frame = 0

Query: 65  ADPRQTEAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIV 124
           A P + ++IGVCY   GNNLP P EVV LY+SNNI +MR+Y PN A L ALR SNI+V++
Sbjct: 21  AVPTRVQSIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLL 80

Query: 125 DIPNSDLEHMV-NLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSF 184
           D+P SD++ +  N S+A +W++RN++AY P V FRYIA+G  + P    AQY+LPAM + 
Sbjct: 81  DVPRSDVQSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNI 140

Query: 185 YSAISAANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLA 244
           Y+A+S+A LQ+QIKVST +   +L  +S+PPS  +FSS A  ++ PIV FL  NG+PLL 
Sbjct: 141 YNALSSAGLQNQIKVSTAVDTGVL-GTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLV 200

Query: 245 NVYPYFIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGV 304
           NVYPYF YT     ISL YALF  S  + V DG F Y +LF+++VDA++ ALE+ GGA V
Sbjct: 201 NVYPYFSYTGNPGQISLPYALFTASG-VVVQDGRFSYQNLFDAIVDAVFAALERVGGANV 260

Query: 305 SIVISESGWPS-GGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKS 364
           ++V+SESGWPS GGG  A+  NA  Y +NLI  V GGTPRRP + IE Y+F MF+EN K+
Sbjct: 261 AVVVSESGWPSAGGGAEASTSNARTYNQNLIRHVGGGTPRRPGKEIEAYIFEMFNENQKA 320

Query: 365 LEMEKHFGLFTSDKKQKYQLSF 385
             +E++FGLF  +K+  YQ+SF
Sbjct: 321 GGIEQNFGLFYPNKQPVYQISF 340

BLAST of Sed0024414 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 335.1 bits (858), Expect = 1.0e-90
Identity = 172/318 (54.09%), Postives = 229/318 (72.01%), Query Frame = 0

Query: 71  EAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSD 130
           +++GVCY   GNNLP   +VVQLY+S NI +MR+Y PN A L ALRGSNIEV++ +PNSD
Sbjct: 31  QSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQALRGSNIEVMLGVPNSD 90

Query: 131 LEHM-VNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKP---SDPAAQYVLPAMSSFYSA 190
           L+++  N S+A NWV+RN+  + P VKFRYIA+G  V P   +    +Y+LPAM +  +A
Sbjct: 91  LQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGTSSLTRYLLPAMRNIRNA 150

Query: 191 ISAANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVY 250
           IS+A LQ+ IKVS+ + ++L+ N SFPPS  SF ++   FI+PI+ F+ +  SPLL N+Y
Sbjct: 151 ISSAGLQNNIKVSSSVDMTLIGN-SFPPSQGSFRNDVRSFIDPIIGFVRRINSPLLVNIY 210

Query: 251 PYFIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIV 310
           PYF Y    R ISL YALF  +P + V DG+  Y +LF++M DA+Y AL ++GG  + IV
Sbjct: 211 PYFSYAGNPRDISLPYALF-TAPNVVVQDGSLGYRNLFDAMSDAVYAALSRAGGGSIEIV 270

Query: 311 ISESGWPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKSLEME 370
           +SESGWPS G  AAT  NA  YY+NLI  V+ G+PRRP + IETYLFAMFDEN K+ E+E
Sbjct: 271 VSESGWPSAGAFAATTNNAATYYKNLIQHVKRGSPRRPNKVIETYLFAMFDENNKNPELE 330

Query: 371 KHFGLFTSDKKQKYQLSF 385
           KHFGLF+ +K+ KY LSF
Sbjct: 331 KHFGLFSPNKQPKYPLSF 346

BLAST of Sed0024414 vs. ExPASy Swiss-Prot
Match: P52407 (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis OX=3981 GN=HGN1 PE=1 SV=2)

HSP 1 Score: 328.2 bits (840), Expect = 1.3e-88
Identity = 171/317 (53.94%), Postives = 222/317 (70.03%), Query Frame = 0

Query: 73  IGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSDLE 132
           +GVCY   GNNLP   EV+ LY+ +NI +MRIY PN A+L ALRGSNIE+I+ +PNSDL+
Sbjct: 38  VGVCYGMQGNNLPPVSEVIALYKKSNITRMRIYDPNRAVLEALRGSNIELILGVPNSDLQ 97

Query: 133 HMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPA----AQYVLPAMSSFYSAIS 192
            + N S+A +WV++N+  +   V FRYIA+G  + P +      AQ+VLPAM + + AI 
Sbjct: 98  SLTNPSNAKSWVQKNVRGFWSSVLFRYIAVGNEISPVNRGTAWLAQFVLPAMRNIHDAIR 157

Query: 193 AANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPY 252
           +A LQDQIKVST I ++L+ N S+PPS  +F  +   +++PI+ FL    SPLLAN+YPY
Sbjct: 158 SAGLQDQIKVSTAIDLTLVGN-SYPPSAGAFRDDVRSYLDPIIGFLSSIRSPLLANIYPY 217

Query: 253 FIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVIS 312
           F Y    R ISL YALF  SP + V DG   Y +LF++ +DALY ALE++ G  + +V+S
Sbjct: 218 FTYAYNPRDISLPYALF-TSPSVVVWDGQRGYKNLFDATLDALYSALERASGGSLEVVVS 277

Query: 313 ESGWPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKSLEMEKH 372
           ESGWPS G  AAT +N   Y  NLI  V+GGTP+RP RAIETYLFAMFDEN K  E+EKH
Sbjct: 278 ESGWPSAGAFAATFDNGRTYLSNLIQHVKGGTPKRPNRAIETYLFAMFDENKKQPEVEKH 337

Query: 373 FGLFTSDKKQKYQLSFS 386
           FGLF  +K QKY L+FS
Sbjct: 338 FGLFFPNKWQKYNLNFS 352

BLAST of Sed0024414 vs. ExPASy Swiss-Prot
Match: Q8VZJ2 (Probable glucan endo-1,3-beta-glucosidase At4g16260 OS=Arabidopsis thaliana OX=3702 GN=At4g16260 PE=1 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 6.3e-88
Identity = 165/322 (51.24%), Postives = 225/322 (69.88%), Query Frame = 0

Query: 66  DPRQTEAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVD 125
           +P   E++GVCY   GNNLP   + + L++ NNI ++R+Y PN A LNALR + IEVI+ 
Sbjct: 17  NPTSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIG 76

Query: 126 IPNSDLEHMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYS 185
           +PN+DL  + N SSA +W++ N+L Y P V F+YIA+G  V PS+     VLPAM + Y 
Sbjct: 77  VPNTDLRSLTNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSN-GGDVVLPAMRNVYD 136

Query: 186 AISAANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANV 245
           A+  ANLQD+IKVST I ++L+ N SFPPS+  F  +   +I+P++ FL    S LLAN+
Sbjct: 137 ALRGANLQDRIKVSTAIDMTLIGN-SFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANI 196

Query: 246 YPYFIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSI 305
           YPYF Y D  R ISL YALF  SP + V DG+  Y +LF++++D +Y A+E+SGG  + +
Sbjct: 197 YPYFSYVDNPRDISLSYALF-TSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPV 256

Query: 306 VISESGWPSGGGTAATLENAGMYYRNLISFVR--GGTPRRPRRAIETYLFAMFDENLKSL 365
           V+SESGWPS GG AA+ +NA  +Y NL S VR   GTP+RP R +ETYLFAMFDEN KS 
Sbjct: 257 VVSESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKSP 316

Query: 366 EMEKHFGLFTSDKKQKYQLSFS 386
           E+EK+FGLF  +K+ K+ ++FS
Sbjct: 317 EIEKNFGLFFPNKQPKFPITFS 335

BLAST of Sed0024414 vs. ExPASy TrEMBL
Match: A0A6J1HBJ9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.9e-152
Identity = 291/376 (77.39%), Postives = 321/376 (85.37%), Query Frame = 0

Query: 15  ILSLLFLGALISPGFI----HTMEMEIEDVD-LSLLPFGSASAIRTGSFLRLPQCADPRQ 74
           +L L  L +L+   FI      M M IEDVD ++LLPFGSAS  R GSF RLPQC  PR+
Sbjct: 7   VLLLASLISLVFHRFIGLKSGNMVMAIEDVDSMALLPFGSAS--RIGSFFRLPQCGGPRR 66

Query: 75  TEAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNS 134
           TE+IGVCY++ GNNLPD REVVQLY+ + I KMRIY+P+ AILNALRGSNIEVIV IPN+
Sbjct: 67  TESIGVCYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVGIPNT 126

Query: 135 DLEHMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYSAISA 194
           DLE +VNLSSA+NWV+RNI AYVPH+KFRY+A+G  V+PSD  A+YVLPA+SS  SAISA
Sbjct: 127 DLERIVNLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISA 186

Query: 195 ANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYF 254
           ANLQDQIKVSTVISISLLSNSSFPPS SSFSSEA GFIEPIV FL KNGSPLLANVYPYF
Sbjct: 187 ANLQDQIKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYF 246

Query: 255 IYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISE 314
            YTDITRTISLDYALFMQSP L + D NFEYNSLFESMVDALYVALEKSGGA VSIVI+E
Sbjct: 247 TYTDITRTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEVSIVIAE 306

Query: 315 SGWPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKSLEMEKHF 374
           SGWPS G  AAT+ENAG YYRNL++FV  GTPRRPRRAIETYLFAMFDENLK +EMEKHF
Sbjct: 307 SGWPSNGSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHF 366

Query: 375 GLFTSDKKQKYQLSFS 386
           GLFT+DKK KYQLSFS
Sbjct: 367 GLFTADKKSKYQLSFS 380

BLAST of Sed0024414 vs. ExPASy TrEMBL
Match: A0A6J1DLI3 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021653 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 8.1e-147
Identity = 291/392 (74.23%), Postives = 321/392 (81.89%), Query Frame = 0

Query: 11  MASSI------LSLLFLGALISPGF--------IHTMEMEIEDVD-LSLLPFGSASAIRT 70
           MASSI      +SLL LGALIS GF           M + IE+VD ++LLPFGSAS I  
Sbjct: 1   MASSIFSKFCSVSLLLLGALISAGFRIFGLKSGNMAMALAIEEVDSMALLPFGSASGI-- 60

Query: 71  GSFLRLPQCADPRQT--EAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILN 130
            SF+RLPQC  PR T  E++GVCY+S G+NLP   EVVQLY++N+I KMRIY+P+ AILN
Sbjct: 61  DSFVRLPQCGGPRPTVAESVGVCYASSGSNLPSATEVVQLYKNNSIGKMRIYNPSEAILN 120

Query: 131 ALRGSNIEVIVDIPNSDLEHMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAA 190
           AL+GSNIEV+V IPN+DLEH+VNLSSA NWV+RNI AY+ HVKFRYIAIG  V PSD  A
Sbjct: 121 ALQGSNIEVVVGIPNTDLEHVVNLSSAANWVQRNIQAYISHVKFRYIAIGNDVHPSDSFA 180

Query: 191 QYVLPAMSSFYSAISAANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSF 250
            YVLPA+ S Y AISAANLQDQIKVSTVISISLLSNSSFPPS  SFSSEA GFIEPIVSF
Sbjct: 181 GYVLPAIRSIYGAISAANLQDQIKVSTVISISLLSNSSFPPSIGSFSSEARGFIEPIVSF 240

Query: 251 LVKNGSPLLANVYPYFIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYV 310
           L +NGSPLLANVYPYF YTDI RTISLDYALFMQSP LA+ DGNF YNSLFESMVDALY 
Sbjct: 241 LAENGSPLLANVYPYFTYTDIARTISLDYALFMQSPALAIRDGNFMYNSLFESMVDALYA 300

Query: 311 ALEKSGGAGVSIVISESGWPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLF 370
           ALEKSGG  VSIVISESGWPS  GTAAT+ENAG YYRNLI+FV+ GTPRRP RAIETYLF
Sbjct: 301 ALEKSGGNEVSIVISESGWPSSEGTAATIENAGTYYRNLINFVQSGTPRRPGRAIETYLF 360

Query: 371 AMFDENLKSLEMEKHFGLFTSDKKQKYQLSFS 386
           +MFDENLK  E+EKHFGLFT +KK KYQLSFS
Sbjct: 361 SMFDENLKPPEIEKHFGLFTPNKKPKYQLSFS 390

BLAST of Sed0024414 vs. ExPASy TrEMBL
Match: A0A6J1H9Q0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 8.1e-139
Identity = 258/315 (81.90%), Postives = 282/315 (89.52%), Query Frame = 0

Query: 71  EAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSD 130
           E+IGVCY++ GNNLPD REVVQLY+ + I KMRIY+P+ AILNALRGSNIEVIV IPN+D
Sbjct: 6   ESIGVCYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVGIPNTD 65

Query: 131 LEHMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYSAISAA 190
           LE +VNLSSA+NWV+RNI AYVPH+KFRY+A+G  V+PSD  A+YVLPA+SS  SAISAA
Sbjct: 66  LERIVNLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAA 125

Query: 191 NLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYFI 250
           NLQDQIKVSTVISISLLSNSSFPPS SSFSSEA GFIEPIV FL KNGSPLLANVYPYF 
Sbjct: 126 NLQDQIKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFT 185

Query: 251 YTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISES 310
           YTDITRTISLDYALFMQSP L + D NFEYNSLFESMVDALYVALEKSGGA VSIVI+ES
Sbjct: 186 YTDITRTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEVSIVIAES 245

Query: 311 GWPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKSLEMEKHFG 370
           GWPS G  AAT+ENAG YYRNL++FV  GTPRRPRRAIETYLFAMFDENLK +EMEKHFG
Sbjct: 246 GWPSNGSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFG 305

Query: 371 LFTSDKKQKYQLSFS 386
           LFT+DKK KYQLSFS
Sbjct: 306 LFTADKKSKYQLSFS 320

BLAST of Sed0024414 vs. ExPASy TrEMBL
Match: A0A6J1DNI9 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021658 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 2.2e-123
Identity = 255/398 (64.07%), Postives = 293/398 (73.62%), Query Frame = 0

Query: 11  MASSI------LSLLFLGALISPGF----------IHTMEMEIEDVD-LSLLPFGSASAI 70
           MASS+      LSLL LGALIS GF             M M I++VD ++LLP GS S I
Sbjct: 1   MASSLFSTFCSLSLLLLGALISAGFRIIGSKSGNMAMAMAMAIDEVDSMALLPLGSVSGI 60

Query: 71  RTGSFLRLPQCADPRQT----EAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNI 130
             GSF+RLPQC  PR T    +A+GVCYSS G+NLP+P EVVQLY+S  I +MRIY  N 
Sbjct: 61  --GSFIRLPQCGGPRPTVETVQAVGVCYSSMGDNLPEPGEVVQLYKSYGIKRMRIYDTNK 120

Query: 131 AILNALRGSNIEVIVDIPNSDLEHMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPS 190
           AILNALRGSNIEV+V IPN+DL+ +VNLSSA +WV+RNI AY+PHV FRY+ IG  V+PS
Sbjct: 121 AILNALRGSNIEVVVGIPNNDLQRVVNLSSAESWVQRNIQAYIPHVNFRYVTIGNEVRPS 180

Query: 191 DPAAQYVLPAMSSFYSAISAANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEP 250
           D  A YVLPAM + YSAISAANLQDQ+KVSTVIS  LL  +S+PPS  SF S+A  FIEP
Sbjct: 181 DSFAGYVLPAMRNIYSAISAANLQDQMKVSTVISTVLL-GASYPPSAGSFRSDAREFIEP 240

Query: 251 IVSFLVKNGSPLLANVYPYFIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVD 310
           IVSFL KNGSPLLAN+YPY+ Y    R I+LDYAL     F  V DGN  Y++L ++ VD
Sbjct: 241 IVSFLAKNGSPLLANLYPYYSYISNNRDITLDYALLYDYNFPTVVDGNLVYHNLLDATVD 300

Query: 311 ALYVALEKSGGAGVSIVISESGWPSGGGTAATLEN--AGMYYRNLISFVRGGTPRRPRRA 370
           ALY ALEKSGGA  SIVISESGWPSG GT   +EN  AG YYRNLI  V+ G+PRRP RA
Sbjct: 301 ALYAALEKSGGANFSIVISESGWPSGAGTNTVVENEVAGTYYRNLIRHVQSGSPRRPGRA 360

Query: 371 IETYLFAMFDENLKSLEMEKHFGLFTSDKKQKYQLSFS 386
           IETYLF+MFDENL+  E EKHFGLFT D KQKYQLS S
Sbjct: 361 IETYLFSMFDENLRPSETEKHFGLFTHDMKQKYQLSVS 395

BLAST of Sed0024414 vs. ExPASy TrEMBL
Match: A0A6J1DNH1 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 4.6e-118
Identity = 243/389 (62.47%), Postives = 291/389 (74.81%), Query Frame = 0

Query: 5   SFQASPMASSILSLLFLGALISPGF-IHTMEMEIEDVD-LSLLPFGSASAIRTGSFLRLP 64
           S  AS +  S   LL LGA ISPG     + + IE+VD ++LLPFGSAS I  GSF+RLP
Sbjct: 6   SLMASSLFCSASLLLLLGAFISPGSKSGNVALAIEEVDSMALLPFGSASGI--GSFVRLP 65

Query: 65  QCADPRQT----EAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGS 124
           QC  PR T    +AIGVCYS+ G+NLP+P EVV+LY+S  I +MRIY  +  ILNALRGS
Sbjct: 66  QCGGPRPTVETVQAIGVCYSTKGDNLPEPGEVVRLYKSLGIKRMRIYDTDQTILNALRGS 125

Query: 125 NIEVIVDIPNSDLEHMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLP 184
           NIEV+V IPN+DLE + N+SSA NWV+ NI A+VPHVKFRYI +G  V+PSD  A YVLP
Sbjct: 126 NIEVVVGIPNTDLERVANVSSAANWVQMNIQAFVPHVKFRYITVGNEVQPSDSFAGYVLP 185

Query: 185 AMSSFYSAISAANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNG 244
           AM + YSAISAANLQDQ+K+STVIS +LL  +S PPS  SF S+A  FIEPIVSFL K G
Sbjct: 186 AMRNIYSAISAANLQDQMKISTVISTALL-GTSHPPSAGSFGSDAREFIEPIVSFLAKKG 245

Query: 245 SPLLANVYPYFIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKS 304
           SPLLAN+Y Y+ Y  ++R ++LDYAL  +S  + + DGN EY++  ESM+DALY ALE S
Sbjct: 246 SPLLANLYTYYSYIGLSRQMNLDYALLKESQGV-IADGNLEYHNYVESMIDALYAALENS 305

Query: 305 GGAGVSIVISESGWPSGGGTAATLEN--AGMYYRNLISFVRGGTPRRPRRAIETYLFAMF 364
           GGA VSIVISESGWPSG GT   +E+  A  YY NLI  V+ GTPRRP RAIETYLF++F
Sbjct: 306 GGANVSIVISESGWPSGAGTNTIIESVVAETYYVNLIRCVQSGTPRRPGRAIETYLFSIF 365

Query: 365 DENLKSLEMEKHFGLFTSDKKQKYQLSFS 386
           DENL+  EMEKHFGLFT D + KYQ SFS
Sbjct: 366 DENLRPSEMEKHFGLFTHDMEPKYQFSFS 390

BLAST of Sed0024414 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 325.9 bits (834), Expect = 4.5e-89
Identity = 165/322 (51.24%), Postives = 225/322 (69.88%), Query Frame = 0

Query: 66  DPRQTEAIGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVD 125
           +P   E++GVCY   GNNLP   + + L++ NNI ++R+Y PN A LNALR + IEVI+ 
Sbjct: 17  NPTSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIG 76

Query: 126 IPNSDLEHMVNLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYS 185
           +PN+DL  + N SSA +W++ N+L Y P V F+YIA+G  V PS+     VLPAM + Y 
Sbjct: 77  VPNTDLRSLTNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSN-GGDVVLPAMRNVYD 136

Query: 186 AISAANLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANV 245
           A+  ANLQD+IKVST I ++L+ N SFPPS+  F  +   +I+P++ FL    S LLAN+
Sbjct: 137 ALRGANLQDRIKVSTAIDMTLIGN-SFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANI 196

Query: 246 YPYFIYTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSI 305
           YPYF Y D  R ISL YALF  SP + V DG+  Y +LF++++D +Y A+E+SGG  + +
Sbjct: 197 YPYFSYVDNPRDISLSYALF-TSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPV 256

Query: 306 VISESGWPSGGGTAATLENAGMYYRNLISFVR--GGTPRRPRRAIETYLFAMFDENLKSL 365
           V+SESGWPS GG AA+ +NA  +Y NL S VR   GTP+RP R +ETYLFAMFDEN KS 
Sbjct: 257 VVSESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKSP 316

Query: 366 EMEKHFGLFTSDKKQKYQLSFS 386
           E+EK+FGLF  +K+ K+ ++FS
Sbjct: 317 EIEKNFGLFFPNKQPKFPITFS 335

BLAST of Sed0024414 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 285.0 bits (728), Expect = 8.8e-77
Identity = 157/315 (49.84%), Postives = 207/315 (65.71%), Query Frame = 0

Query: 73  IGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSDLE 132
           IGVCY   GNNL    EVV LYQ  NI +MR+Y PN   LNALRGSNIE+++D+PN DL+
Sbjct: 35  IGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNALRGSNIELVLDVPNPDLQ 94

Query: 133 HMV-NLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYSAISAAN 192
            +  + + A  WV+ N+  Y  +V FRYI++G  V+PSD AA +VLPAM +   A+S+  
Sbjct: 95  RLASSQAEADTWVRNNVRNYA-NVTFRYISVGNEVQPSDQAASFVLPAMQNIERAVSSLG 154

Query: 193 LQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYFIY 252
               IKVST I    +  S FPPS+ +F+ E   FI P++SFL    SPLL N YPYF Y
Sbjct: 155 ----IKVSTAIDTRGI--SGFPPSSGTFTPEFRSFIAPVISFLSSKQSPLLVNNYPYFSY 214

Query: 253 TDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISESG 312
           T   R I LDY LF  +P   V+DG  +Y +LF +++D +Y +LEK+GG  + IV+SESG
Sbjct: 215 TGNMRDIRLDYTLF-TAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGGSLEIVVSESG 274

Query: 313 WPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLK-SLEMEKHFG 372
           WP+ GG A  ++NA  Y  NLI  V+ G+PRRP RA ETY+FAMFDEN K   E EK +G
Sbjct: 275 WPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIFAMFDENSKQGPETEKFWG 334

Query: 373 LFTSDKKQKYQLSFS 386
           LF  + + KY ++F+
Sbjct: 335 LFLPNLQPKYVVNFN 341

BLAST of Sed0024414 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 278.5 bits (711), Expect = 8.2e-75
Identity = 150/315 (47.62%), Postives = 208/315 (66.03%), Query Frame = 0

Query: 73  IGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSDLE 132
           +GVCY   GNNLP P E + L++  NI ++R+Y P+  +L ALRGSNIEV + +PNS L+
Sbjct: 27  VGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYLQ 86

Query: 133 HMVNLSSATN-WVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYSAISAAN 192
            + +  S  N WV+  ++ Y   V+FRYI++G  VK SD  AQ+++PAM +   A+ AA 
Sbjct: 87  SVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAAG 146

Query: 193 LQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYFIY 252
           L  +IKVST + + +L   S+PPS  SF  +    +EPI+ FLV   SPLL N+Y YF Y
Sbjct: 147 LGGRIKVSTSVDMGVL-RESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 206

Query: 253 TDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISESG 312
                 I LDYALF  +P   V D    Y +LF++M+DA+Y ALEKSGGA + IV++E+G
Sbjct: 207 AGNVGQIRLDYALF-TAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETG 266

Query: 313 WPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKSLE--MEKHF 372
           WP+GGGT   +ENA +Y  NLI  V+ GTP+RP + IETYLFA++DEN K     +EK +
Sbjct: 267 WPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKFW 326

Query: 373 GLFTSDKKQKYQLSF 385
           GLF  +K+ KY ++F
Sbjct: 327 GLFYPNKQPKYDINF 339

BLAST of Sed0024414 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 275.4 bits (703), Expect = 6.9e-74
Identity = 146/314 (46.50%), Postives = 206/314 (65.61%), Query Frame = 0

Query: 73  IGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSDLE 132
           IGVCY   G+ LP P +VV LY+  NI +MR+Y P+   L ALRGS+IE+I+D+P+SDLE
Sbjct: 32  IGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGALAALRGSDIELILDVPSSDLE 91

Query: 133 HMV-NLSSATNWVKRNILAYVPHVKFRYIAIGYVVKPSDPAAQYVLPAMSSFYSAISAAN 192
            +  + + A  WV+ N+ +Y   V+FRYI +G  VKPS     ++L AM +  +A+S A 
Sbjct: 92  RLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKPS--VGGFLLQAMQNIENAVSGAG 151

Query: 193 LQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYFIY 252
           L  ++KVST I+    +++S PPS   F  E   F+EP++ FL    SPLL N+YPYF Y
Sbjct: 152 L--EVKVSTAIATDTTTDTS-PPSQGRFRDEYKSFLEPVIGFLASKQSPLLVNLYPYFSY 211

Query: 253 TDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISESG 312
              T  I LDYALF     +  +D  + Y +LF++ +D++Y ALEKSGG  + IV+SE+G
Sbjct: 212 MGDTANIHLDYALFTAQSTVD-NDPGYSYQNLFDANLDSVYAALEKSGGGSLEIVVSETG 271

Query: 313 WPSGGGTAATLENAGMYYRNLISFVRGGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGL 372
           WP+ G    ++ENA  Y  NLI  V+ G+PRRP +AIETY+FAMFDEN K    EK +GL
Sbjct: 272 WPTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETYIFAMFDENKKEPTYEKFWGL 331

Query: 373 FTSDKKQKYQLSFS 386
           F  D++ KY+++F+
Sbjct: 332 FHPDRQSKYEVNFN 339

BLAST of Sed0024414 vs. TAIR 10
Match: AT4G29360.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 221.9 bits (564), Expect = 9.1e-58
Identity = 127/321 (39.56%), Postives = 193/321 (60.12%), Query Frame = 0

Query: 73  IGVCYSSFGNNLPDPREVVQLYQSNNINKMRIYHPNIAILNALRGSNIEVIVDIPNSDLE 132
           IG+CY    +NLP P  V +L Q  NI  +RIY  NI +L A   + IE+++ +PN+DL 
Sbjct: 27  IGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADLL 86

Query: 133 HMVNL-SSATNWVKRNILAYVPHVKFRYIAIGY-VVKPSDPAAQYVLPAMSSFYSAISAA 192
                 S+   W+  NIL Y P  K   I++G  V +  D A   VLPAM + ++A+  +
Sbjct: 87  AFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTALKKS 146

Query: 193 NLQDQIKVSTVISISLLSNSSFPPSNSSFSSEADGFIEPIVSFLVKNGSPLLANVYPYFI 252
            L  +IK+S+  S+++LS  SFPPS++SFS +   F++P++ FLV+N SP + ++YPY+ 
Sbjct: 147 GLDKKIKISSSHSLAILSR-SFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLYPYYA 206

Query: 253 YTDITRTISLDYALFMQSPFLAVHDGNFEYNSLFESMVDALYVALEKSGGAGVSIVISES 312
           Y D T  + L+YALF  S  +        Y+++F++ +DA+Y AL       V ++++ES
Sbjct: 207 YRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVTES 266

Query: 313 GWPSGGG---TAATLENAGMYYRNLISFVRG--GTPRRPRRAIETYLFAMFDENLK-SLE 372
           GWPS G    TAAT ENA  Y  NLI  V G  GTP +P   I+ YLF++F+EN K  +E
Sbjct: 267 GWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPGIE 326

Query: 373 MEKHFGLFTSDKKQKYQLSFS 386
            E+++G+F ++    Y L F+
Sbjct: 327 SERNWGMFYANGTNVYALDFT 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004139045.12.0e-15576.70glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KAE8653516.1 hypothetical pr... [more]
XP_038880622.13.7e-15478.65glucan endo-1,3-beta-glucosidase-like [Benincasa hispida][more]
KAG6590190.16.0e-15280.23Lichenase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022961205.16.0e-15277.39glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata][more]
KAG7023843.13.9e-15180.17Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma... [more]
Match NameE-valueIdentityDescription
A7PQW32.4e-9557.81Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120... [more]
O223171.0e-9053.73Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2[more]
P079791.0e-9054.09Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3[more]
P524071.3e-8853.94Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis O... [more]
Q8VZJ26.3e-8851.24Probable glucan endo-1,3-beta-glucosidase At4g16260 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1HBJ92.9e-15277.39(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 P... [more]
A0A6J1DLI38.1e-14774.23(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021653 ... [more]
A0A6J1H9Q08.1e-13981.90(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 P... [more]
A0A6J1DNI92.2e-12364.07(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021658 ... [more]
A0A6J1DNH14.6e-11862.47(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 ... [more]
Match NameE-valueIdentityDescription
AT4G16260.14.5e-8951.24Glycosyl hydrolase superfamily protein [more]
AT3G57240.18.8e-7749.84beta-1,3-glucanase 3 [more]
AT3G57270.18.2e-7547.62beta-1,3-glucanase 1 [more]
AT3G57260.16.9e-7446.50beta-1,3-glucanase 2 [more]
AT4G29360.29.1e-5839.56O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 73..384
e-value: 7.8E-106
score: 354.2
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 303..316
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 71..385
e-value: 1.0E-119
score: 401.2
NoneNo IPR availablePANTHERPTHR32227:SF267GLUCAN ENDO-1,3-BETA-GLUCOSIDASEcoord: 65..382
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 65..382
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 73..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0024414.1Sed0024414.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006749 glutathione metabolic process
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds