Sed0023675 (gene) Chayote v1

Overview
NameSed0023675
Typegene
OrganismSechium edule (Chayote v1)
DescriptionProlyl-tRNA synthetase
LocationLG03: 6343054 .. 6348581 (-)
RNA-Seq ExpressionSed0023675
SyntenySed0023675
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATGGCTTTGTTGTCAAACTAATCCATCCCTGTCCTGTGCTGCATCTCACAACCCTCAAATTTCTCCTCGTAATTCAACACGGTTGGCGCCTCCTCGCATTTAGGCAGCGCCGCCGCAGCGTCGCAGTTCGCCGTTCATCGGAATCGCAGAGCTCGATCGATCCATTGTAAGCTTCACTCTCTCTTTGTCACTTCAATTGCACTGCTCCCTCTTATCTCTTTATACATTCTCATCATGCTCTCATTCAGCGTGTCTGAAGTTTGTTGAGTATCTTCGTAATTCTTCAATGTTCATAGTGTTGTTCCTTTAGATTTTCTTAATCTGAACCATATTTTCTCCAATTTGGCTGTTTGTTGTTGGAATCATATAAAAAACTGAATGCTTTTTGTTATATTTTCTCCAACCTATATCGCCCTGGCGGCGCGGTTAATTAGGCGCACACCTTTTAGCGCCTTACATGAAGCCCTGGGCGCGCGCCTTGTGGGCTTTTGTGTTTCCTTATGGTTTTTTAGGGTTTATGCCTCAATCAGCGAGACCTCGGCTCATTTTTACATGATAAATGTTGTTAGAATTTCTACGAATGTGTACTTGATTATTATTGAGTTGTATTTTTGTTGTTGCTACATTTGAACTTTTTACGATGGTTCTCCTGCAGAAGTTAAGCTGAAAATATGGCTGGTCCTAAGCCTGGTAGCTCTGCTACTAATCCAAAAGCTGGTAAGTTTTGTTTCTTTATTACGGCAGCTGTTTGTGCAGTTGTTTCTTATTGCCTTGTTAGGTTGTGTTTACCAATGCTTTGTTGTTTACTGGGCAGGCGGGAAAAAGAAGGAGGTGAAGAAAGAGACTGGCCTAGGTCTTACTAACAAGAAGGATGATAACTTTGGAGAGTGGTATTCTGAGGTGCTGTTTCTTGCTTGATTTTTGTTGTCTTCATTGCAAAATGAATTTTTGTTTGGTAAACTTGATCACATATGAGTTTGACCTTGATGTTAGACATCCTTTGTACTGAAGGTTGATTGTGTAACTCACTCCTTTGCTGTCAAAGTACATATCTCAGATCATGTGAAGTTATTCATATGAGTTTAGTTTTTGTTCTCATTATAATTTAAATTGACAAAGCTTGAACAGTTTTCCCTTCAACTTTGGAGAGATGTGTTTTATTTTTGTTCACAAGATTTTATTGATTCGTTTAACTTTTTCTAATTTTTTGTTGTTGTTATAAAATGTCATTTATATTGGAATCATTGATAATGTCTAATAAATAAGAAGGAAAAAAATTAACAAATCAATAAAGATATTGTTTTTTTTGGAGGAAGTTCCCCAGTAGTACTTTGAACAACTTCTTCTCGGTGACTTGAATAATTATTTTTCTAGTTTTTCTAGGTGGTTGTCAGTGGAGAAATGATCGAGTATTATGATATCTCTGGCTGTTATATACTGAGGCCGTGGGCAATGTCTATCTGGGAGACTATGCAAGTAAGGATCCCACTCTTTCTATTGCCAATTTTACAATCTTTTCTTGTTTTGATGTTCAGAGTTTTAATTTGTCACCGGCACAGGTATTTTTTGATGCTGAAATTAAGAAGATGAAAATCAAGAACTGCTATTTTCCACTTTTTGTATCTCCGGGCGTCTTACAAAGAGAGAAAGATCATATTGAAGGTTTTGCTCCTGAGGTGACATTTCATTATTCAAATGCAACAATAGAGAAACTTCTCCATCTGAATTTGAAATGCTTATTTATTATGTTACCATTCCTAATGTTCTCTCTGTTAAATTTTATATTTCAAGTTTATAAAGGTGCAATTGTGGTCTAGTATTGACAATTTTGGTGTTTGATATCATTTTGAAGGTTGCCTGGGTGACAAAGTCTGGGGAATCTGACTTGGAAGTGCCTATTGCAATTCGCCCTACAAGTGAAACGGTGATGTATCCATACTATTCGAAATGGATCAGGGGCCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAGTTCAGCAATCCCACACCATTTATTAGGCAAGTACTCTTGCATCCGCTTTTTTCCTTTTTTTTCTTTTCCTTTCACTATGGTTATTGACTGCATTGGACCTATTAACAGTTGGTTGTTTTTATAATTTTGTTCAATGTTTTTGGTTAAGTGTGTCTTATTAGCATATGTCGTATCTTCTTGAAGTTTTTTAAGAAAGTTCTGAGAACTTTATTGTTTTGAAATTGTTTTAAACCATTTGGAGAAAAAGACTAATTTTTATTTACTACTGACTGCAGGAGTCGTGAGTTCCTTTGGCAGGAAGGGCATACTGCTTTTGCTACCAAGGATGAAGCAGATACAGAGGTGATACTCTAGAACTTTAAACAGGTGTAAACGAAATTGTTATGTCCTATTAAGTAAATTACGACTTAGAAAAATGCTTTTGTCATGGGTTGCTTGGTTCTATAGTTTTGCTACCAACTTGTCTTTTTCTCAGTGTATAGTGATGAAAATGACCCAAACAGTTTTGGGTTAGTGTGGAAATTTCACAATCTTTAAATGGGAAGGGTAGAGGTCTGAGGAATTGAATTTCTCTTTGTTATTTGGTCTCCTTTGGTTAACTGTTTTGTGTAAAAATAATGAAATTAGTAGATTTTACCATCCTGCCTTTAGATTTTGAGATGCACGATCACCGGTGGTTCATGCATTTTAGGATGCTTCTTTATGAAGAAAAAGTGAAAAAGGATGTGTTAATGATTTTGAGAAAAAAAAAGTGCATGCATTCGAAAAGAAGGACAAAGATCCTTGAAATGCTAGGATGCTGCTATGGGAAAAAATGGAGTTGTTGGTTTTTAAGGGTTTTTAGGACTTACCGAGTAGGACTCGGTAGAGGCTAGGTACAGCTGCTACTTGGTTTGTGACCGGGGGTTTCACTTTTTTTTTCCCCTCAATTTTTAGGGCACAGTTTCTCACATTTAATAACATACTTGGATTCGAGGTATTGAAAATTCTAAACGTTTACACATATATGAAAAATAAATTTAAATATCAAGTCCCCAACATGTCCTCGTCCTACTTTTATAGAAATTGACGTGTTGTTGTGTTTGTGTCGTGTCATAGTGTGTAACTGTGATTGCTATTTAAAGATTACTTTTATTTCAGGTTCTTGAGATATTGGAACTGTATAGACGAGTATATGAAGAGTACCTTGCCATTCCTGTTATCAAGGGCAAGAAGAGTGAGATGGAAAAGTTCGCTGGTGGCCTTTACACCACAAGTGTTGAGGTATAATTTGAAGAAATGTTATAATTCTTATGACTTTGGGGAATTGATATCCGTTGCTGGTTTAAGTTGTATAAAACCATTTCTTCACATCTGATATGCAGGCATTTATCCCAAACACTGGCCGTGGAATTCAGGGTGCAACTTCCCATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAAATTAACTTTGAAAATGAAAAGGGAGAGAAAGCTATGGTCTGGCAAAATTCATGGGCCTATAGTACTAGAACGGTAATAATTTGATTCTTCCATCTGTCTTTGATAACTTGTCAGAGCGAATTTCTGCATTTGTTAAACAACTAGTTATGGGGAGGCCAAATATGACTTTTTGTGAACATTCTGTTCTTTTATCTTTTTATCCAGTCTTTCTCTTTCCAAACTGTTTGCAGATTGGTGTGATGGTTATGGTTCATGGGGATGACAAGGGATTGGTGCTGCCTCCCAAAGTTGCATCAGTTCAAGTTGTTATAGTTCCTGTTCCTTACAAAGATGCGGATACTCAGGGAATTTTCGATGCTTGTTCTGCCACTTTGGATACGTTGTCCGAAGCAGGAATTCGTGCAGAGGTAGACTCTAGAGATAACTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAAGGTGTTCCACTCAGGATTGAAATAGGACCCAAGGACTTGGCAAACAATCAGGTTACATATTGTCCATTAGTAGTATTTTTTGGTCAATACTGAATACTGTTTCTCCACCGCTATTAATTAGTTGGATCATGTTGATACGTAGTGCTCATATATAAGTATGTACTTTATTTCTAGGTACGTGCTGTTCGTCGTGATAATTTGACAAAGAAGGACATACCTAGAGATTCATTGGTTGAACAAGTGAAAGAGTTACTTGAAAGTATTCAACAAAGCCTGTTTGATGCGGCAAAAGTAAAACGAGACGCATGCATTCAAGTTATTAATACTTGGGAAGAGTTTACTGAAGCACTCAGTCTGAAGAAAATGATATTAGCTCCATGGTGCGATGAAGAGGTATAGCTTCTCAGTGTTCTTATTGATGTTTTTATTCTGCTTGTGAAGGATTGGCTGCTTTTTGGTTCTGACTTTTCATCAGGCCACCATAATTTTATTTGATTAGTTGAAGGGATTCTTACGGTTATGGTTTTCATATATTACTCAATGCGCTTTCATATGCTGGGATTTGTTAGATGTTTGAATTCTAATGAAGTCAAATAAGAGCAATAGTTTGTCCCCAAGGGGTGGCGCAGTGGTTGAAGACTTGGGGGCTTTGAGGGTATGCTTTCCTCATGGTCCCATGTTCAAGACTCACCTGTGACATTACTCTTTCGATGTCTCCAGTGTCTGACCTAGGGACTGGTTACCCTTGTTTAAAAAAAAAGAACAATAGTTCAGTAACATAAGTGTGTACTATGAGCTAGAGGTCATGGGTTCGAATCCTCCCACTTGAGGGTTGTTGAACTTGAAAAAAAAATATAATGAAGTTTGTGTTAAGCTTCAGAAATGTGAAATTTATATAGTATTTGTTATATGCCAATCTTTTTCATCTGCTGGTGTACTTTTTTTTTTTTTTTTTTTCTCAAGGATGTTTTCATTGTTATGATTCTTCAGGAGGTTGAGAAGGACGTGAAAACAAGGACCAAGGGTGAGATGGGAGCAGCTAAAACCTTATGTTCTCCATTCGAGCAGCCCGCACTCCTCGAAGGTACTAAATTTATGTCTGAATCCGTATTCCTTCTTTTGAATGGAATGTATATCATCTTGTGACATCTTGGAAAGTTTATTGACAACTTTTTTTGGTTCCTTGAATTACCATGATGATGATCTGGATTCAGGTACCAAATGTTTCGCATCTGGAAAGCCTGCAAAAAAATGGAGCTATTGGGGCCGAAGCTACTAAGTTATCAGCTCGTGTATATCTCCTATGCAATGCGATGCAATGCGATGCTTGGATACGTGTTCTTGAGTATGGGGGCATTCAATCCAGTTAATTTCGAGTTCAATTGTTAAAAAAGCATTCACAAAGAAAGTTACTAGACAACATTTTTTTTACTCTTTGCATACTTGGACCTTTTTTCATATACAATATTTGGGTTTTGCCTTACAATCTTTGTTTTAAGTTCTGTTAAATTAATCCAACTCTTGGAATTTTCAATTTTCAATTTTGTTTGTTTTGTTAATGTACATGAATCTGGGGAAATCACACGGGAAACCTGTCGTCAC

mRNA sequence

AAAATGGCTTTGTTGTCAAACTAATCCATCCCTGTCCTGTGCTGCATCTCACAACCCTCAAATTTCTCCTCGTAATTCAACACGGTTGGCGCCTCCTCGCATTTAGGCAGCGCCGCCGCAGCGTCGCAGTTCGCCGTTCATCGGAATCGCAGAGCTCGATCGATCCATTAAGTTAAGCTGAAAATATGGCTGGTCCTAAGCCTGGTAGCTCTGCTACTAATCCAAAAGCTGGCGGGAAAAAGAAGGAGGTGAAGAAAGAGACTGGCCTAGGTCTTACTAACAAGAAGGATGATAACTTTGGAGAGTGGTATTCTGAGGTGGTTGTCAGTGGAGAAATGATCGAGTATTATGATATCTCTGGCTGTTATATACTGAGGCCGTGGGCAATGTCTATCTGGGAGACTATGCAAGTATTTTTTGATGCTGAAATTAAGAAGATGAAAATCAAGAACTGCTATTTTCCACTTTTTGTATCTCCGGGCGTCTTACAAAGAGAGAAAGATCATATTGAAGGTTTTGCTCCTGAGGTTGCCTGGGTGACAAAGTCTGGGGAATCTGACTTGGAAGTGCCTATTGCAATTCGCCCTACAAGTGAAACGGTGATGTATCCATACTATTCGAAATGGATCAGGGGCCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAGTTCAGCAATCCCACACCATTTATTAGGAGTCGTGAGTTCCTTTGGCAGGAAGGGCATACTGCTTTTGCTACCAAGGATGAAGCAGATACAGAGGTTCTTGAGATATTGGAACTGTATAGACGAGTATATGAAGAGTACCTTGCCATTCCTGTTATCAAGGGCAAGAAGAGTGAGATGGAAAAGTTCGCTGGTGGCCTTTACACCACAAGTGTTGAGGCATTTATCCCAAACACTGGCCGTGGAATTCAGGGTGCAACTTCCCATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAAATTAACTTTGAAAATGAAAAGGGAGAGAAAGCTATGGTCTGGCAAAATTCATGGGCCTATAGTACTAGAACGATTGGTGTGATGGTTATGGTTCATGGGGATGACAAGGGATTGGTGCTGCCTCCCAAAGTTGCATCAGTTCAAGTTGTTATAGTTCCTGTTCCTTACAAAGATGCGGATACTCAGGGAATTTTCGATGCTTGTTCTGCCACTTTGGATACGTTGTCCGAAGCAGGAATTCGTGCAGAGGTAGACTCTAGAGATAACTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAAGGTGTTCCACTCAGGATTGAAATAGGACCCAAGGACTTGGCAAACAATCAGGTACGTGCTGTTCGTCGTGATAATTTGACAAAGAAGGACATACCTAGAGATTCATTGGTTGAACAAGTGAAAGAGTTACTTGAAAGTATTCAACAAAGCCTGTTTGATGCGGCAAAAGTAAAACGAGACGCATGCATTCAAGTTATTAATACTTGGGAAGAGTTTACTGAAGCACTCAGTCTGAAGAAAATGATATTAGCTCCATGGTGCGATGAAGAGGAGGTTGAGAAGGACGTGAAAACAAGGACCAAGGGTGAGATGGGAGCAGCTAAAACCTTATGTTCTCCATTCGAGCAGCCCGCACTCCTCGAAGGTACCAAATGTTTCGCATCTGGAAAGCCTGCAAAAAAATGGAGCTATTGGGGCCGAAGCTACTAAGTTATCAGCTCGTGTATATCTCCTATGCAATGCGATGCAATGCGATGCTTGGATACGTGTTCTTGAGTATGGGGGCATTCAATCCAGTTAATTTCGAGTTCAATTGTTAAAAAAGCATTCACAAAGAAAGTTACTAGACAACATTTTTTTTACTCTTTGCATACTTGGACCTTTTTTCATATACAATATTTGGGTTTTGCCTTACAATCTTTGTTTTAAGTTCTGTTAAATTAATCCAACTCTTGGAATTTTCAATTTTCAATTTTGTTTGTTTTGTTAATGTACATGAATCTGGGGAAATCACACGGGAAACCTGTCGTCAC

Coding sequence (CDS)

ATGGCTGGTCCTAAGCCTGGTAGCTCTGCTACTAATCCAAAAGCTGGCGGGAAAAAGAAGGAGGTGAAGAAAGAGACTGGCCTAGGTCTTACTAACAAGAAGGATGATAACTTTGGAGAGTGGTATTCTGAGGTGGTTGTCAGTGGAGAAATGATCGAGTATTATGATATCTCTGGCTGTTATATACTGAGGCCGTGGGCAATGTCTATCTGGGAGACTATGCAAGTATTTTTTGATGCTGAAATTAAGAAGATGAAAATCAAGAACTGCTATTTTCCACTTTTTGTATCTCCGGGCGTCTTACAAAGAGAGAAAGATCATATTGAAGGTTTTGCTCCTGAGGTTGCCTGGGTGACAAAGTCTGGGGAATCTGACTTGGAAGTGCCTATTGCAATTCGCCCTACAAGTGAAACGGTGATGTATCCATACTATTCGAAATGGATCAGGGGCCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAGTTCAGCAATCCCACACCATTTATTAGGAGTCGTGAGTTCCTTTGGCAGGAAGGGCATACTGCTTTTGCTACCAAGGATGAAGCAGATACAGAGGTTCTTGAGATATTGGAACTGTATAGACGAGTATATGAAGAGTACCTTGCCATTCCTGTTATCAAGGGCAAGAAGAGTGAGATGGAAAAGTTCGCTGGTGGCCTTTACACCACAAGTGTTGAGGCATTTATCCCAAACACTGGCCGTGGAATTCAGGGTGCAACTTCCCATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAAATTAACTTTGAAAATGAAAAGGGAGAGAAAGCTATGGTCTGGCAAAATTCATGGGCCTATAGTACTAGAACGATTGGTGTGATGGTTATGGTTCATGGGGATGACAAGGGATTGGTGCTGCCTCCCAAAGTTGCATCAGTTCAAGTTGTTATAGTTCCTGTTCCTTACAAAGATGCGGATACTCAGGGAATTTTCGATGCTTGTTCTGCCACTTTGGATACGTTGTCCGAAGCAGGAATTCGTGCAGAGGTAGACTCTAGAGATAACTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAAGGTGTTCCACTCAGGATTGAAATAGGACCCAAGGACTTGGCAAACAATCAGGTACGTGCTGTTCGTCGTGATAATTTGACAAAGAAGGACATACCTAGAGATTCATTGGTTGAACAAGTGAAAGAGTTACTTGAAAGTATTCAACAAAGCCTGTTTGATGCGGCAAAAGTAAAACGAGACGCATGCATTCAAGTTATTAATACTTGGGAAGAGTTTACTGAAGCACTCAGTCTGAAGAAAATGATATTAGCTCCATGGTGCGATGAAGAGGAGGTTGAGAAGGACGTGAAAACAAGGACCAAGGGTGAGATGGGAGCAGCTAAAACCTTATGTTCTCCATTCGAGCAGCCCGCACTCCTCGAAGGTACCAAATGTTTCGCATCTGGAAAGCCTGCAAAAAAATGGAGCTATTGGGGCCGAAGCTACTAA

Protein sequence

MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSLFDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALLEGTKCFASGKPAKKWSYWGRSY
Homology
BLAST of Sed0023675 vs. NCBI nr
Match: XP_038907011.1 (proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907020.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907029.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1029.6 bits (2661), Expect = 9.0e-297
Identity = 499/511 (97.65%), Postives = 503/511 (98.43%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYR +YEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRCIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDN  KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAK KRD CIQVINTWEEFTEAL  KKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKEKRDKCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
           SPFEQPAL EGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Sed0023675 vs. NCBI nr
Match: XP_008453976.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >TYK16984.1 proline--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1028.5 bits (2658), Expect = 2.0e-296
Identity = 496/511 (97.06%), Postives = 505/511 (98.83%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATN KAGGKKKEVKKETGLGLTNKKD+NFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNQKAGGKKKEVKKETGLGLTNKKDENFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRR+YEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFEN+KGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACSATLDTL++AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTDAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDN  KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKVKRD CIQVINTWEEFTEALS KKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
           SPF+QPAL EGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Sed0023675 vs. NCBI nr
Match: XP_023543704.1 (proline--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1027.3 bits (2655), Expect = 4.4e-296
Identity = 498/511 (97.46%), Postives = 501/511 (98.04%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSA  PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRR+YEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACS TLDTLSEAGIRAEVD+RDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDN  KKDIPR SLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLESIQQSL 420

Query: 421 FDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKVKRD CIQVINTWEEFTEALS KKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
           SPFEQP L EGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Sed0023675 vs. NCBI nr
Match: XP_004152099.2 (proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis sativus] >KGN53085.1 hypothetical protein Csa_014802 [Cucumis sativus])

HSP 1 Score: 1026.2 bits (2652), Expect = 9.9e-296
Identity = 496/511 (97.06%), Postives = 503/511 (98.43%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATN KAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNKKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIK+MKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKQMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRR+YEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACSATLDTL+ AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTAAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDN  KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKVKRD CIQVINTWEEFTEAL  KKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
           SPF+QPAL EGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Sed0023675 vs. NCBI nr
Match: KAG6601922.1 (Proline--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1026.2 bits (2652), Expect = 9.9e-296
Identity = 497/511 (97.26%), Postives = 501/511 (98.04%), Query Frame = 0

Query: 1    MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
            MAGPKPGSSA  PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 616  MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 675

Query: 61   YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
            YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 676  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 735

Query: 121  SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSRE 180
            SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSRE
Sbjct: 736  SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 795

Query: 181  FLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
            FLWQEGHTAFATKDEADTEVLEILELYRR+YEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 796  FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 855

Query: 241  AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
            AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 856  AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 915

Query: 301  DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360
            DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACS TLDTLSEAGIRAEVD+RDNYSPG
Sbjct: 916  DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 975

Query: 361  WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSL 420
            WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDN  KKDIPR SLVEQVKELL+SIQQSL
Sbjct: 976  WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLDSIQQSL 1035

Query: 421  FDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
            FDAAKVKRD CIQVINTWEEFTEALS KKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 1036 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 1095

Query: 481  SPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
            SPFEQP L EGTKCFASGKPAKKWSYWGRSY
Sbjct: 1096 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 1126

BLAST of Sed0023675 vs. ExPASy Swiss-Prot
Match: Q9M1R2 (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 3.2e-257
Identity = 417/497 (83.90%), Postives = 457/497 (91.95%), Query Frame = 0

Query: 15  AGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETM 74
           +G KKK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE M
Sbjct: 35  SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIM 94

Query: 75  QVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRP 134
           Q+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRP
Sbjct: 95  QIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRP 154

Query: 135 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKD 194
           TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATK 
Sbjct: 155 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKA 214

Query: 195 EADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGAT 254
           EAD EVL+ILELYRR+YEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGAT
Sbjct: 215 EADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGAT 274

Query: 255 SHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASV 314
           SHCLGQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASV
Sbjct: 275 SHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASV 334

Query: 315 QVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLR 374
           QVV++PVPYKDA+TQGI+DAC+AT   L EAGIRAE D RDNYSPGWKYS WEMKGVPLR
Sbjct: 335 QVVVIPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLR 394

Query: 375 IEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSLFDAAKVKRDACIQV 434
           IEIGP+DL N+QVR VRRDN  K+DIPR SLVE VKELLE IQQ++++ AK KR+AC+Q 
Sbjct: 395 IEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQE 454

Query: 435 INTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALLEGTKC 494
           + TW+EF +AL+ KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP L EGT C
Sbjct: 455 VKTWDEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLC 514

Query: 495 FASGKPAKKWSYWGRSY 512
           FASGKPAKKW+YWGRSY
Sbjct: 515 FASGKPAKKWTYWGRSY 530

BLAST of Sed0023675 vs. ExPASy Swiss-Prot
Match: O60155 (Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prs1 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 3.4e-166
Identity = 297/546 (54.40%), Postives = 371/546 (67.95%), Query Frame = 0

Query: 2   AGPKPGSSATNPKAGGKKKEVKKETG-------LGLTNKKDDNFGEWYSEVVVSGEMIEY 61
           + P   ++ + P A  KK E  K          +G+T +KD +F  WY +V+   +MIEY
Sbjct: 172 SAPGSATAPSKPAAQKKKAEPSKNDAAIENAALIGITVRKDADFPNWYQQVLTKSDMIEY 231

Query: 62  YDISGCYILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE 121
           YDISGCYIL+PW+ SIWE +Q +FD EIKK+ ++N YFPLFVS  VL++EKDH+EGFAPE
Sbjct: 232 YDISGCYILKPWSYSIWEAIQGWFDKEIKKLGVRNGYFPLFVSSKVLEKEKDHVEGFAPE 291

Query: 122 VAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTP 181
           VAWVT++G S+L+ PIAIRPTSETVMYPYY+KWIR HRDLPLKLNQW +VVRWEF NP P
Sbjct: 292 VAWVTRAGTSELDEPIAIRPTSETVMYPYYAKWIRSHRDLPLKLNQWNSVVRWEFKNPQP 351

Query: 182 FIRSREFLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGL 241
           F+R+REFLWQEGHTA  T + A  EV +IL+LY R+Y + LA+PVIKG KSE EKFAGG+
Sbjct: 352 FLRTREFLWQEGHTAHMTLEGATEEVHQILDLYARIYTDLLAVPVIKGVKSENEKFAGGM 411

Query: 242 YTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGE--------KAMVWQNSWA 301
           +TT+VE +IP TGRGIQGATSHCLGQNF+KMF I  E+   E        K  VWQNSW 
Sbjct: 412 FTTTVEGYIPTTGRGIQGATSHCLGQNFSKMFNIVVEDPNAEIGPTGERPKLFVWQNSWG 471

Query: 302 YSTRTIGVMVMVHGDDKGLVLPPKVASVQVVIVPV----PYKDADTQGIFDACSATLDTL 361
            STRTIGV VMVHGDDKGL LPP +A VQ V+VP        D +   I   CS   D L
Sbjct: 472 LSTRTIGVAVMVHGDDKGLKLPPAIALVQSVVVPCGITNKTTDQERNEIEGFCSKLADRL 531

Query: 362 SEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPR 421
           + A IR E D R  Y+PG+K+SHWEMKGVPLR+E GP D   NQV AVRRD   K  +P 
Sbjct: 532 NAADIRTEADLR-AYTPGYKFSHWEMKGVPLRLEYGPNDAKKNQVTAVRRDTFEKIPVPL 591

Query: 422 DSLVEQVKELLESIQQSLFDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVE 481
           ++L + V +LL  IQ ++++ AK +RDA +  +  W +F  AL+ K +++ PWC+  E E
Sbjct: 592 NNLEKGVSDLLAKIQTNMYETAKAERDAHVVKVKEWADFVPALNKKNIVMIPWCNTTECE 651

Query: 482 KDVKTRT------------KGEMGAAKTLCSPFEQP----ALLEG-TKCFASGKPAKKWS 512
           K++K  +            K     AK+LC P EQP    A++EG TKC   G  AK W 
Sbjct: 652 KEIKKNSARQVNGDEPEDEKAPSMGAKSLCIPLEQPSGEDAIIEGTTKCAGCGNLAKVWG 711

BLAST of Sed0023675 vs. ExPASy Swiss-Prot
Match: P28668 (Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 GN=GluProRS PE=1 SV=2)

HSP 1 Score: 572.8 bits (1475), Expect = 3.9e-162
Identity = 288/527 (54.65%), Postives = 371/527 (70.40%), Query Frame = 0

Query: 2    AGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 61
            A PKP        A      VKK+T LGL   K+DN  +WYS+V+  GEMIEYYD+SGCY
Sbjct: 1189 AQPKPAKPVKKEPAADASGAVKKQTRLGLEATKEDNLPDWYSQVITKGEMIEYYDVSGCY 1248

Query: 62   ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 121
            ILR W+ +IW+ ++ +FDAEI +M +K CYFP+FVS  VL++EK HI  FAPEVAWVTKS
Sbjct: 1249 ILRQWSFAIWKAIKTWFDAEITRMGVKECYFPIFVSKAVLEKEKTHIADFAPEVAWVTKS 1308

Query: 122  GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREF 181
            G+SDL  PIA+RPTSETVMYP Y+KW++ +RDLP++LNQW NVVRWEF  PTPF+R+REF
Sbjct: 1309 GDSDLAEPIAVRPTSETVMYPAYAKWVQSYRDLPIRLNQWNNVVRWEFKQPTPFLRTREF 1368

Query: 182  LWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 241
            LWQEGHTAFA K+EA  EVL+IL+LY  VY   LAIPV+KG+K+E EKFAGG YTT+VEA
Sbjct: 1369 LWQEGHTAFADKEEAAKEVLDILDLYALVYTHLLAIPVVKGRKTEKEKFAGGDYTTTVEA 1428

Query: 242  FIPNTGRGIQGATSHCLGQNFAKMFEINFEN-EKGEKAMVWQNSWAYSTRTIGVMVMVHG 301
            FI  +GR IQGATSH LGQNF+KMFEI +E+ E  +K  V+QNSW  +TRTIGVM+MVH 
Sbjct: 1429 FISASGRAIQGATSHHLGQNFSKMFEIVYEDPETQQKKYVYQNSWGITTRTIGVMIMVHA 1488

Query: 302  DDKGLVLPPKVASVQVVIVP----VPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDN 361
            D++GLVLPP VA +Q ++VP    V  KD +   + DAC A    L   G+R E D RDN
Sbjct: 1489 DNQGLVLPPHVACIQAIVVPCGITVNTKDDERAQLLDACKALEKRLVGGGVRCEGDYRDN 1548

Query: 362  YSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESI 421
            YSPGWK++HWE+KGVPLR+E+GPKDL   Q+ AVRRD + K  IP   + +++  LLE+I
Sbjct: 1549 YSPGWKFNHWELKGVPLRLEVGPKDLKAQQLVAVRRDTVEKITIPLADVEKKIPALLETI 1608

Query: 422  QQSLFDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRT----KGE 481
             +S+ + A+    +  + +  W +F   L  K ++LAP+C E   E  +K  +    + E
Sbjct: 1609 HESMLNKAQEDMTSHTKKVTNWTDFCGFLEQKNILLAPFCGEISCEDKIKADSARGEEAE 1668

Query: 482  MGA----AKTLCSPFEQPA-LLEGTKCF---ASGKPAKKWSYWGRSY 512
             GA    AK+LC PF+QPA +    KC     + KP K ++ +GRSY
Sbjct: 1669 PGAPAMGAKSLCIPFDQPAPIAASDKCINPSCTNKP-KFYTLFGRSY 1714

BLAST of Sed0023675 vs. ExPASy Swiss-Prot
Match: Q8CGC7 (Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE=1 SV=4)

HSP 1 Score: 571.6 bits (1472), Expect = 8.8e-162
Identity = 289/522 (55.36%), Postives = 370/522 (70.88%), Query Frame = 0

Query: 5    KPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILR 64
            K   S  +    G+ +  KK+T LGL  KK++N  EWYS+V+   EMIEYYD+SGCYILR
Sbjct: 991  KSQGSGLSSGGAGEGQGPKKQTRLGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILR 1050

Query: 65   PWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGES 124
            PW+ SIWE+++ FFDAEIKK+ ++NCYFP+FVS   L++EK+HIE FAPEVAWVT+SG++
Sbjct: 1051 PWSYSIWESIKDFFDAEIKKLGVENCYFPIFVSQAALEKEKNHIEDFAPEVAWVTRSGKT 1110

Query: 125  DLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQ 184
            +L  PIAIRPTSETVMYP Y+KW++ HRDLP++LNQWCNVVRWEF +P PF+R+REFLWQ
Sbjct: 1111 ELAEPIAIRPTSETVMYPAYAKWVQSHRDLPVRLNQWCNVVRWEFKHPQPFLRTREFLWQ 1170

Query: 185  EGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIP 244
            EGH+AFAT +EA  EVL+ILELY RVYEE LAIPV++G+K+E EKFAGG YTT++EAFI 
Sbjct: 1171 EGHSAFATFEEAADEVLQILELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTIEAFIS 1230

Query: 245  NTGRGIQGATSHCLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDD 304
             +GR IQGATSH LGQNF+KM EI FE+ K  GEK   +Q SW  +TRTIGVMVMVHGD+
Sbjct: 1231 ASGRAIQGATSHHLGQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIGVMVMVHGDN 1290

Query: 305  KGLVLPPKVASVQVVIVPVPYKDA----DTQGIFDACSATLDTLSEAGIRAEVDSRDNYS 364
             GLVLPP+VASVQVV++P    +A    D + +   C+     L  A IR  VD RDNYS
Sbjct: 1291 MGLVLPPRVASVQVVVIPCGITNALSEEDREALMAKCNEYRRRLLGANIRVRVDLRDNYS 1350

Query: 365  PGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQ 424
            PGWK++HWE+KGVP+R+E+GP+D+ + Q  AVRRD   K  I       +++++LE IQ 
Sbjct: 1351 PGWKFNHWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAEAKLEKVLEDIQL 1410

Query: 425  SLFDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKG----EMG 484
            +LF  A       + V NT E+F + L   K+   P+C E + E  +K  T      E G
Sbjct: 1411 NLFTRASEDLKTHMVVSNTLEDFQKVLDAGKVAQIPFCGEIDCEDWIKKMTARDQDVEPG 1470

Query: 485  A----AKTLCSPFEQPALLE-GTKCFASGKPAKKWSYWGRSY 512
            A    AK+LC PF     L+ G  C     PAK ++ +GRSY
Sbjct: 1471 APSMGAKSLCIPFNPLCELQPGAMCVCGKNPAKFYTLFGRSY 1512

BLAST of Sed0023675 vs. ExPASy Swiss-Prot
Match: P07814 (Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=1 SV=5)

HSP 1 Score: 571.2 bits (1471), Expect = 1.1e-161
Identity = 282/510 (55.29%), Postives = 364/510 (71.37%), Query Frame = 0

Query: 17   GKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQV 76
            G+ +  KK+T LGL  KK++N  +WYS+V+   EMIEY+DISGCYILRPWA +IWE ++ 
Sbjct: 1003 GEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKD 1062

Query: 77   FFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTS 136
            FFDAEIKK+ ++NCYFP+FVS   L++EK H+  FAPEVAWVT+SG+++L  PIAIRPTS
Sbjct: 1063 FFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTS 1122

Query: 137  ETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKDEA 196
            ETVMYP Y+KW++ HRDLP+KLNQWCNVVRWEF +P PF+R+REFLWQEGH+AFAT +EA
Sbjct: 1123 ETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEA 1182

Query: 197  DTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSH 256
              EVL+IL+LY +VYEE LAIPV+KG+K+E EKFAGG YTT++EAFI  +GR IQG TSH
Sbjct: 1183 AEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSH 1242

Query: 257  CLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASV 316
             LGQNF+KMFEI FE+ K  GEK   +QNSW  +TRTIGVM MVHGD+ GLVLPP+VA V
Sbjct: 1243 HLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACV 1302

Query: 317  QVVIVPVPYKDA----DTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKG 376
            QVVI+P    +A    D + +   C+     L    IR   D RDNYSPGWK++HWE+KG
Sbjct: 1303 QVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKG 1362

Query: 377  VPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSLFDAAKVKRDA 436
            VP+R+E+GP+D+ + Q  AVRRD   K  +  +    +++ +LE IQ +LF  A      
Sbjct: 1363 VPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKT 1422

Query: 437  CIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKG----EMGA----AKTLCSP 496
             + V NT E+F + L   K++  P+C E + E  +K  T      E GA    AK+LC P
Sbjct: 1423 HMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIP 1482

Query: 497  FEQPALLE-GTKCFASGKPAKKWSYWGRSY 512
            F+    L+ G KC     PAK ++ +GRSY
Sbjct: 1483 FKPLCELQPGAKCVCGKNPAKYYTLFGRSY 1512

BLAST of Sed0023675 vs. ExPASy TrEMBL
Match: A0A5D3D175 (Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001250 PE=3 SV=1)

HSP 1 Score: 1028.5 bits (2658), Expect = 9.7e-297
Identity = 496/511 (97.06%), Postives = 505/511 (98.83%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATN KAGGKKKEVKKETGLGLTNKKD+NFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNQKAGGKKKEVKKETGLGLTNKKDENFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRR+YEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFEN+KGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACSATLDTL++AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTDAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDN  KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKVKRD CIQVINTWEEFTEALS KKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
           SPF+QPAL EGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Sed0023675 vs. ExPASy TrEMBL
Match: A0A1S3BXN6 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103494536 PE=3 SV=1)

HSP 1 Score: 1028.5 bits (2658), Expect = 9.7e-297
Identity = 496/511 (97.06%), Postives = 505/511 (98.83%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATN KAGGKKKEVKKETGLGLTNKKD+NFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNQKAGGKKKEVKKETGLGLTNKKDENFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRR+YEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFEN+KGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACSATLDTL++AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTDAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDN  KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKVKRD CIQVINTWEEFTEALS KKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
           SPF+QPAL EGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Sed0023675 vs. ExPASy TrEMBL
Match: A0A0A0KTZ1 (Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 4.8e-296
Identity = 496/511 (97.06%), Postives = 503/511 (98.43%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATN KAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNKKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIK+MKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKQMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRR+YEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACSATLDTL+ AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTAAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDN  KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKVKRD CIQVINTWEEFTEAL  KKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
           SPF+QPAL EGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Sed0023675 vs. ExPASy TrEMBL
Match: A0A6J1GYJ1 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 4.8e-296
Identity = 497/511 (97.26%), Postives = 501/511 (98.04%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSA  PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRR+YEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACS TLDTLSEAGIRAEVD+RDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDN  KKDIPR SLVEQVKELL+SIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLDSIQQSL 420

Query: 421 FDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKVKRD CIQVINTWEEFTEALS KKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
           SPFEQP L EGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Sed0023675 vs. ExPASy TrEMBL
Match: A0A6J1JLY7 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111487085 PE=3 SV=1)

HSP 1 Score: 1024.6 bits (2648), Expect = 1.4e-295
Identity = 496/511 (97.06%), Postives = 500/511 (97.85%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSA  PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRR+YEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACS TLDTLSEAGIRAEVD+RDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDN  KKDIPR SLVEQVKELL+SIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLDSIQQSL 420

Query: 421 FDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKVKRD CIQVINTWEEFTEAL  KKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
           SPFEQP L EGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Sed0023675 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 888.6 bits (2295), Expect = 2.3e-258
Identity = 417/497 (83.90%), Postives = 457/497 (91.95%), Query Frame = 0

Query: 15  AGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETM 74
           +G KKK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE M
Sbjct: 35  SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIM 94

Query: 75  QVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRP 134
           Q+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRP
Sbjct: 95  QIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRP 154

Query: 135 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKD 194
           TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATK 
Sbjct: 155 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKA 214

Query: 195 EADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGAT 254
           EAD EVL+ILELYRR+YEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGAT
Sbjct: 215 EADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGAT 274

Query: 255 SHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASV 314
           SHCLGQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASV
Sbjct: 275 SHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASV 334

Query: 315 QVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLR 374
           QVV++PVPYKDA+TQGI+DAC+AT   L EAGIRAE D RDNYSPGWKYS WEMKGVPLR
Sbjct: 335 QVVVIPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLR 394

Query: 375 IEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSLFDAAKVKRDACIQV 434
           IEIGP+DL N+QVR VRRDN  K+DIPR SLVE VKELLE IQQ++++ AK KR+AC+Q 
Sbjct: 395 IEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQE 454

Query: 435 INTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALLEGTKC 494
           + TW+EF +AL+ KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP L EGT C
Sbjct: 455 VKTWDEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLC 514

Query: 495 FASGKPAKKWSYWGRSY 512
           FASGKPAKKW+YWGRSY
Sbjct: 515 FASGKPAKKWTYWGRSY 530

BLAST of Sed0023675 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 888.6 bits (2295), Expect = 2.3e-258
Identity = 417/497 (83.90%), Postives = 457/497 (91.95%), Query Frame = 0

Query: 15  AGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETM 74
           +G KKK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE M
Sbjct: 35  SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIM 94

Query: 75  QVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRP 134
           Q+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRP
Sbjct: 95  QIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRP 154

Query: 135 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKD 194
           TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATK 
Sbjct: 155 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKA 214

Query: 195 EADTEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGAT 254
           EAD EVL+ILELYRR+YEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGAT
Sbjct: 215 EADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGAT 274

Query: 255 SHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASV 314
           SHCLGQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASV
Sbjct: 275 SHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASV 334

Query: 315 QVVIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLR 374
           QVV++PVPYKDA+TQGI+DAC+AT   L EAGIRAE D RDNYSPGWKYS WEMKGVPLR
Sbjct: 335 QVVVIPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLR 394

Query: 375 IEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLESIQQSLFDAAKVKRDACIQV 434
           IEIGP+DL N+QVR VRRDN  K+DIPR SLVE VKELLE IQQ++++ AK KR+AC+Q 
Sbjct: 395 IEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQE 454

Query: 435 INTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALLEGTKC 494
           + TW+EF +AL+ KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP L EGT C
Sbjct: 455 VKTWDEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLC 514

Query: 495 FASGKPAKKWSYWGRSY 512
           FASGKPAKKW+YWGRSY
Sbjct: 515 FASGKPAKKWTYWGRSY 530

BLAST of Sed0023675 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 417.5 bits (1072), Expect = 1.5e-116
Identity = 212/501 (42.32%), Postives = 307/501 (61.28%), Query Frame = 0

Query: 18  KKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVF 77
           K  EV +         +  +F  WY +V+ S E+ +Y  + G  ++RP+  +IWE +Q +
Sbjct: 53  KSSEVDRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTMVIRPYGYAIWEAIQDY 112

Query: 78  FDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSE 137
            + + K+    N YFP F+    +++E  H+EGF+PE+A VT  G  +LE  + +RPTSE
Sbjct: 113 LNVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELEEKLVVRPTSE 172

Query: 138 TVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKDEAD 197
           T++   +++WI  +RDLPL +NQW NV RWE     PFIR+ EFLWQEGHTA AT +EA+
Sbjct: 173 TIVNHMFTQWIHSYRDLPLMINQWANVTRWEM-RTKPFIRTLEFLWQEGHTAHATPEEAE 232

Query: 198 TEVLEILELYRRVYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHC 257
            E  +++E+Y R   E  AIPVI G+KS++E FAG   T ++EA + +  + +Q  TSH 
Sbjct: 233 KEAKQMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEAMMGDR-KALQAGTSHN 292

Query: 258 LGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVV 317
           LGQNF++ F   F +E GE+  VWQ SWA STR +G ++M HGDD GL+LPPK+A +QVV
Sbjct: 293 LGQNFSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGDDTGLMLPPKIAPIQVV 352

Query: 318 IVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEI 377
           IVP+  KD +  G+  A S+  + L  AG+R ++D  D  +PGWK++ WEMKG+PLRIEI
Sbjct: 353 IVPIWKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGWKFNFWEMKGIPLRIEI 412

Query: 378 GPKDLANNQVRAVRRDNLTKK------DIPRDSLVEQVKELLESIQQSLFDAAKVKRDAC 437
           GP+D+++N V   RRD   K        +   +LV  VKE L+ IQ SL + A   RD+ 
Sbjct: 413 GPRDVSSNSVVVSRRDVPGKAGKVFGISMEPSTLVAYVKEKLDEIQTSLLEKALSFRDSN 472

Query: 438 IQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALLEG 497
           I  +N++ E  +A+S  K    PW   +  E+ VK  T      A   C PFEQ    +G
Sbjct: 473 IVDVNSYAELKDAISSGKWARGPWSASDADEQRVKEET-----GATIRCFPFEQ---TQG 532

Query: 498 TK-CFASGKPAKKWSYWGRSY 512
           TK C  +G PA++ + + +SY
Sbjct: 533 TKTCLMTGNPAEEVAIFAKSY 543

BLAST of Sed0023675 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 286.6 bits (732), Expect = 4.0e-77
Identity = 145/221 (65.61%), Postives = 165/221 (74.66%), Query Frame = 0

Query: 297 MVHGDDKGLVLPPKVASVQVVIVPVPYKD-ADTQGIFDACSATLDTLSEAGIRAEVDSRD 356
           M HGDDKGLV PPKVA VQVV++ VP K  AD Q + DAC A   TL  AGIRAE D RD
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 357 NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNLTKKDIPRDSLVEQVKELLES 416
           NYS GWKY+  E+ GVPLRIE GP+DLAN+QVR V RDN  K D+ R  L+EQVK+LLE 
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVKRGDLIEQVKDLLEK 208

Query: 417 IQQSLFDAAKVKRDACIQVINTWEEFTEALSLKKMILAPWCDEEEVEKDVKTRTKGEM-- 476
           IQ +L+D AK K + C Q + TW+EF EALS KK+ILAPWCD+ EVEKDVK RT+G+   
Sbjct: 209 IQSNLYDVAKRKVEECTQKVETWDEFVEALSQKKLILAPWCDKVEVEKDVKRRTRGDETG 268

Query: 477 ---GAAKTLCSPFEQPALLEGTKCFASGKPAKKWSYWGRSY 512
              G AKTLC+P EQP L E T CFASGKPAKKWSYWGRSY
Sbjct: 269 GGGGGAKTLCTPLEQPELGEETLCFASGKPAKKWSYWGRSY 309


HSP 2 Score: 62.0 bits (149), Expect = 1.6e-09
Identity = 27/47 (57.45%), Postives = 37/47 (78.72%), Query Frame = 0

Query: 24 KETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYD-ISGCYILRPWAMS 70
          KET LG+T KKD++FG+WYSE    GE++EYY+ + GCYIL+P  +S
Sbjct: 3  KETRLGITAKKDEDFGKWYSEACRFGELVEYYESVKGCYILKPSGIS 49

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038907011.19.0e-29797.65proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907... [more]
XP_008453976.12.0e-29697.06PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >TYK16984... [more]
XP_023543704.14.4e-29697.46proline--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo][more]
XP_004152099.29.9e-29697.06proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis sativus] >KGN53085.1 hypot... [more]
KAG6601922.19.9e-29697.26Proline--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q9M1R23.2e-25783.90Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P... [more]
O601553.4e-16654.40Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 ... [more]
P286683.9e-16254.65Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 G... [more]
Q8CGC78.8e-16255.36Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE... [more]
P078141.1e-16155.29Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=... [more]
Match NameE-valueIdentityDescription
A0A5D3D1759.7e-29797.06Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A1S3BXN69.7e-29797.06Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103494536 PE=3 SV=1[more]
A0A0A0KTZ14.8e-29697.06Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1[more]
A0A6J1GYJ14.8e-29697.26Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1[more]
A0A6J1JLY71.4e-29597.06Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111487085 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G62120.12.3e-25883.90Class II aaRS and biotin synthetases superfamily protein [more]
AT3G62120.22.3e-25883.90Class II aaRS and biotin synthetases superfamily protein [more]
AT5G52520.11.5e-11642.32Class II aaRS and biotin synthetases superfamily protein [more]
AT5G10880.14.0e-7765.61tRNA synthetase-related / tRNA ligase-related [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002316Proline-tRNA ligase, class IIaPRINTSPR01046TRNASYNTHPROcoord: 160..168
score: 48.97
coord: 89..107
score: 25.15
coord: 130..141
score: 49.38
coord: 170..181
score: 41.36
IPR016061Proline-tRNA ligase, class II, C-terminalSMARTSM00946ProRS_C_1_2coord: 438..511
e-value: 2.2E-26
score: 103.7
IPR016061Proline-tRNA ligase, class II, C-terminalPFAMPF09180ProRS-C_1coord: 438..511
e-value: 3.6E-25
score: 88.0
IPR004499Proline-tRNA ligase, class IIa, archaeal-typeTIGRFAMTIGR00408TIGR00408coord: 32..511
e-value: 4.3E-177
score: 587.7
IPR004499Proline-tRNA ligase, class IIa, archaeal-typePANTHERPTHR43382PROLYL-TRNA SYNTHETASEcoord: 13..511
IPR004499Proline-tRNA ligase, class IIa, archaeal-typeHAMAPMF_01571Pro_tRNA_synth_type3coord: 31..511
score: 38.260265
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 130..296
e-value: 5.0E-18
score: 65.7
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 315..411
e-value: 6.1E-20
score: 71.2
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 31..308
e-value: 7.2E-110
score: 368.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR43382:SF4PROLINE--TRNA LIGASE, CYTOPLASMIC-LIKEcoord: 13..511
NoneNo IPR availableCDDcd00862ProRS_anticodon_zinccoord: 304..511
e-value: 8.09539E-92
score: 276.871
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 303..428
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 36..302
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 309..429
e-value: 3.7E-40
score: 138.5
IPR017449Prolyl-tRNA synthetase, class IIGENE3D3.30.110.30coord: 432..511
e-value: 8.8E-27
score: 95.2
IPR017449Prolyl-tRNA synthetase, class IISUPERFAMILY64586C-terminal domain of ProRScoord: 433..511
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 70..308
score: 18.758682
IPR033721Prolyl-tRNA synthetase, catalytic domainCDDcd00778ProRS_core_arch_eukcoord: 37..298
e-value: 7.56536E-164
score: 462.835

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0023675.1Sed0023675.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006433 prolyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004827 proline-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding