Sed0023022 (gene) Chayote v1

BLAST of Sed0023022 vs. NCBI nr
Match: XP_022947380.1 (Niemann-Pick C1 protein-like [Cucurbita moschata])

HSP 1 Score: 2338.5 bits (6059), Expect = 0.0e+00
Identity = 1195/1302 (91.78%), Postives = 1235/1302 (94.85%), Query Frame = 0

Query: 1    MFLNRGVMASRLRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMYD 60
            MFLNRG MA RLRF +SI L QM+FFVTMLIGGEADLSV V S STSEE+HS EYCAMYD
Sbjct: 1    MFLNRGGMACRLRFRISIFLLQMIFFVTMLIGGEADLSVPVRSASTSEERHSEEYCAMYD 60

Query: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120
            ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP
Sbjct: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120

Query: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLYD 180
            LFVGCPACLRNFLNLFCELSCSPRQSLFINV+SIAEVGGKMTVDGIDY+VTEKFGKGLYD
Sbjct: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDYFVTEKFGKGLYD 180

Query: 181  SCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQM 240
            SCKDVKFGTMNTRAIDFVGGGAKS EE FAFLGQK V PG+PGSPY+INFK N DK SQM
Sbjct: 181  SCKDVKFGTMNTRAIDFVGGGAKSSEEFFAFLGQK-VGPGIPGSPYSINFKANTDKPSQM 240

Query: 241  ELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITI 300
            ELMNVS YSCGDTSLGCSCGDCP SP CSSLEPP PPK NACTIRIWS KSSCIDFSITI
Sbjct: 241  ELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSLKSSCIDFSITI 300

Query: 301  LYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHL 360
            LYV+FIS FLGWALFHPTRERRRFS+ EEPLLNIGDDGE+NSVNLEEN+NG TKEH  HL
Sbjct: 301  LYVIFISAFLGWALFHPTRERRRFSAREEPLLNIGDDGEVNSVNLEENENGATKEHGVHL 360

Query: 361  TLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420
            TL+N VQLSTIQ YI++FYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL
Sbjct: 361  TLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420

Query: 421  WVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNKV 480
            WVGHGS+AA EKQFFD HLAPFYRIEQLIIATKP GR   APRIVTEDNILLLFDIQNKV
Sbjct: 421  WVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDNILLLFDIQNKV 480

Query: 481  NELVANYSGSVVSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYTS 540
            NELVANYSGSVVSL DICLKPL +DCATQSILQY+KMDPENFD+YGGV+H EYC QHYTS
Sbjct: 481  NELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVEHVEYCLQHYTS 540

Query: 541  TETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFI 600
            TETCFSAFKAPLDPSTSLGGFVGNNY+EASAFVVTYPVNNAID VG+EN KAIAWEKAF+
Sbjct: 541  TETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDENGKAIAWEKAFV 600

Query: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSN 660
            KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTI VSYLVMFAYISVALGDSN
Sbjct: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMFAYISVALGDSN 660

Query: 661  ISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMC 720
            ISSSFY+SSKVLLGLSGV+LVVLSVLGS+GFFSA+GVKSTLIIMEVIPFLVLAVGVDNMC
Sbjct: 661  ISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPFLVLAVGVDNMC 720

Query: 721  ILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780
            ILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA
Sbjct: 721  ILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780

Query: 781  LAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSRY 840
            LAV LDFILQLSAFVALIVFDI RAENHRVDCFPCIKVP  SDE NQGFNQ R GLLSRY
Sbjct: 781  LAVFLDFILQLSAFVALIVFDIRRAENHRVDCFPCIKVPPRSDEPNQGFNQRRLGLLSRY 840

Query: 841  MKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLA 900
            MKDVHAPLLGLWGVKIAV++IFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ YFDDLA
Sbjct: 841  MKDVHAPLLGLWGVKIAVVIIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQGYFDDLA 900

Query: 901  EHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPAA 960
            E+LRIGPPLYFVVKDYNYSSKSR+TNQLCSISQCDSNSLLNEISRASLTPEL+YIAKPAA
Sbjct: 901  EYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLTPELNYIAKPAA 960

Query: 961  SWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRHS 1020
            SWLDDFLVWLSPEAFGCCRKFTN SYCPPDDQPPCC PDEG+CD+S GVC+DCTTCF HS
Sbjct: 961  SWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGVCQDCTTCFHHS 1020

Query: 1021 DLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080
            DLVAGRPTTVQF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT
Sbjct: 1021 DLVAGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080

Query: 1081 PLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140
            PLN QGDYVNALRAA+EF +KISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA
Sbjct: 1081 PLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140

Query: 1141 IFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200
            IFIVS VITS LWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV
Sbjct: 1141 IFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200

Query: 1201 HLVHAFSVSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYL 1260
            HLVHAFSVSCGDRSQRAREAL+TMGASVFSGITLTKLVGVIVLCFAKSEIFV+YYFQMYL
Sbjct: 1201 HLVHAFSVSCGDRSQRAREALTTMGASVFSGITLTKLVGVIVLCFAKSEIFVIYYFQMYL 1260

Query: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRSLAIGDVHEETELLVS 1303
            ALVIIGFLHGLVFLPVILSMIGPPSR     D   ETEL VS
Sbjct: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRYSVQDDAPIETELHVS 1301

BLAST of Sed0023022 vs. NCBI nr
Match: XP_023533350.1 (Niemann-Pick C1 protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2337.4 bits (6056), Expect = 0.0e+00
Identity = 1192/1302 (91.55%), Postives = 1236/1302 (94.93%), Query Frame = 0

Query: 1    MFLNRGVMASRLRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMYD 60
            MFLNRG MA RLRF +SI + QM+FFVTMLIGGEADLSV V S STSEE+HS EYCAMYD
Sbjct: 1    MFLNRGGMACRLRFRISIFMLQMIFFVTMLIGGEADLSVPVRSASTSEERHSEEYCAMYD 60

Query: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120
            ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP
Sbjct: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120

Query: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLYD 180
            LFVGCPACLRNFLNLFCELSCSPRQSLFINV+SIAEVGGKMTVDGIDY+VTE+FGKGLYD
Sbjct: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDYFVTEEFGKGLYD 180

Query: 181  SCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQM 240
            SCKDVKFGTMNTRAIDFVGGGAKS EE FAFLGQK V PG+PGSPY+INFK N DK S+M
Sbjct: 181  SCKDVKFGTMNTRAIDFVGGGAKSSEEFFAFLGQK-VGPGIPGSPYSINFKANTDKPSEM 240

Query: 241  ELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITI 300
            ELMNVS YSCGDTSLGCSCGDCP SP CSSLEPP PPK NACTIRIWS KSSCIDFSITI
Sbjct: 241  ELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSLKSSCIDFSITI 300

Query: 301  LYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHL 360
            LYV+FIS FLGWALFHPTRERRR S+ EEPLLNIGDDGE+NSVNLEEN+NG TKEH  HL
Sbjct: 301  LYVIFISAFLGWALFHPTRERRRLSAREEPLLNIGDDGEVNSVNLEENENGATKEHGVHL 360

Query: 361  TLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420
            TL+N VQLSTIQ YI++FYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL
Sbjct: 361  TLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420

Query: 421  WVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNKV 480
            WVGHGS+AA EKQFFD HLAPFYRIEQLIIATKP GR   APRIVTEDNILLLFDIQNKV
Sbjct: 421  WVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDNILLLFDIQNKV 480

Query: 481  NELVANYSGSVVSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYTS 540
            NELVANYSGSVVSL DICLKPL +DCATQSILQY+KMDPENFD+YGGV+H EYC QHYTS
Sbjct: 481  NELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVEHVEYCLQHYTS 540

Query: 541  TETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFI 600
            TETCFSAFKAPLDPSTSLGGFVGNNY+EASAFVVTYPVNNAID VG+EN KAIAWEKAF+
Sbjct: 541  TETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDENGKAIAWEKAFV 600

Query: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSN 660
            KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTI VSYLVMFAYISVALGDSN
Sbjct: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMFAYISVALGDSN 660

Query: 661  ISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMC 720
            ISSSFY+SSKVLLGLSGV+LVVLSVLGS+GFFSA+GVKSTLIIMEVIPFLVLAVGVDNMC
Sbjct: 661  ISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPFLVLAVGVDNMC 720

Query: 721  ILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780
            ILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA
Sbjct: 721  ILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780

Query: 781  LAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSRY 840
            LAV LDFILQLSAFVALIVFDILRAENHRVDCFPCIKVP  SDE NQGFNQ R GLLSRY
Sbjct: 781  LAVFLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPPRSDEPNQGFNQRRLGLLSRY 840

Query: 841  MKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLA 900
            MKDVHAPLLGLWGVKIAV++IFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ YFDDLA
Sbjct: 841  MKDVHAPLLGLWGVKIAVVIIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQGYFDDLA 900

Query: 901  EHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPAA 960
            E+LRIGPPLYFVVKDYNYSSKSR+TNQLCSISQCD+NSLLNEISRASLTPEL+YIAKPAA
Sbjct: 901  EYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDANSLLNEISRASLTPELNYIAKPAA 960

Query: 961  SWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRHS 1020
            SWLDDFLVWLSPEAFGCCRKFTN SYCPPDDQPPCC PDEG+CD+S GVC+DCTTCF HS
Sbjct: 961  SWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGVCQDCTTCFHHS 1020

Query: 1021 DLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080
            DLV GRPTTVQF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT
Sbjct: 1021 DLVTGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080

Query: 1081 PLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140
            PLN QGDYVNALRAA+EF +KISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA
Sbjct: 1081 PLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140

Query: 1141 IFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200
            IFIVS VITS LWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV
Sbjct: 1141 IFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200

Query: 1201 HLVHAFSVSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYL 1260
            HLVHAFSVSCGDRSQRAREAL+TMGASVFSGITLTKLVGVIVLCFAKSEIFV+YYFQMYL
Sbjct: 1201 HLVHAFSVSCGDRSQRAREALTTMGASVFSGITLTKLVGVIVLCFAKSEIFVIYYFQMYL 1260

Query: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRSLAIGDVHEETELLVS 1303
            ALVIIGFLHGLVFLPVILSMIGPPSR L  GD   ETEL VS
Sbjct: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRYLVEGDAPIETELHVS 1301

BLAST of Sed0023022 vs. NCBI nr
Match: XP_022970905.1 (Niemann-Pick C1 protein-like [Cucurbita maxima])

HSP 1 Score: 2332.4 bits (6043), Expect = 0.0e+00
Identity = 1190/1302 (91.40%), Postives = 1234/1302 (94.78%), Query Frame = 0

Query: 1    MFLNRGVMASRLRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMYD 60
            MFLNR  MA RLRF +SI L QM+FFVTMLIGGEADLSV V S STSEE+HS EYCAMYD
Sbjct: 1    MFLNRRGMACRLRFRISIFLMQMIFFVTMLIGGEADLSVPVRSASTSEERHSEEYCAMYD 60

Query: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120
            ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP
Sbjct: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120

Query: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLYD 180
            LFVGCPACLRNFLNLFCELSCSPRQSLFINV+SIAEVGGKMTVDGIDY+VTEKFGKGLYD
Sbjct: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDYFVTEKFGKGLYD 180

Query: 181  SCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQM 240
            SCKDVKFGTMNTRAIDFVGGGAKSFEE FAFLGQK V PG+PGSPY+INFKVN DK SQ+
Sbjct: 181  SCKDVKFGTMNTRAIDFVGGGAKSFEEFFAFLGQK-VGPGIPGSPYSINFKVNTDKPSQV 240

Query: 241  ELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITI 300
            ELMNVS YSCGDTSLGCSCGDCP SP CSSLEPP PPK NACTIRIWS KSSCIDFSITI
Sbjct: 241  ELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSLKSSCIDFSITI 300

Query: 301  LYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHL 360
            LYV+F+S FLGWALFHPTRER RFS+ EEPLLNIGDDGE+NSVNLEEN+NG TKEH  HL
Sbjct: 301  LYVIFVSAFLGWALFHPTRERSRFSAREEPLLNIGDDGEVNSVNLEENENGATKEHGVHL 360

Query: 361  TLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420
            TL+N VQLSTIQ YI++FYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL
Sbjct: 361  TLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420

Query: 421  WVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNKV 480
            WVGHGS+AA EKQFFD HLAPFYRIEQLIIATKP GR   APRIVTEDNILLLFDIQNKV
Sbjct: 421  WVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDNILLLFDIQNKV 480

Query: 481  NELVANYSGSVVSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYTS 540
            NELVANYSGSVVSL DICLKPL +DCATQSILQY+KMDPENFD+YGGV+H EYC QHYTS
Sbjct: 481  NELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVEHVEYCLQHYTS 540

Query: 541  TETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFI 600
            TETCFSAFKAPLDPSTSLGGFVGNNY+EASAFVVTYPVNNAID VG+EN KAIAWEKAF+
Sbjct: 541  TETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDENGKAIAWEKAFV 600

Query: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSN 660
            KL KEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTI VSYLVMFAYISVALGDSN
Sbjct: 601  KLVKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMFAYISVALGDSN 660

Query: 661  ISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMC 720
            ISSSFY+SSKVLLGLSGV+LVVLSVLGS+GFFSA+GVKSTLIIMEVIPFLVLAVGVDNMC
Sbjct: 661  ISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPFLVLAVGVDNMC 720

Query: 721  ILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780
            ILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA
Sbjct: 721  ILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780

Query: 781  LAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSRY 840
            LAV LDFILQLSAFVALIVFDILRAENHRVDCFPCIKVP  SDE NQGFNQ R GLLSRY
Sbjct: 781  LAVFLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPPRSDEPNQGFNQRRLGLLSRY 840

Query: 841  MKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLA 900
            MKDVHAPLLGLWGVKIAV++IFVGLTLGSI LSTKIEVGLEQKIVLPRDSYLQ YFDDLA
Sbjct: 841  MKDVHAPLLGLWGVKIAVVIIFVGLTLGSITLSTKIEVGLEQKIVLPRDSYLQGYFDDLA 900

Query: 901  EHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPAA 960
            E+LRIGPPLYFVVKDYNYSSKSR+TNQLCSISQCDSNSLLNEISRASLTPEL+YIAKPAA
Sbjct: 901  EYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLTPELNYIAKPAA 960

Query: 961  SWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRHS 1020
            SWLDDFLVWLSPEAFGCCRKFTN SYCPPDDQPPCC PDEG+CD+S GVC+DCTTCF HS
Sbjct: 961  SWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGVCQDCTTCFHHS 1020

Query: 1021 DLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080
            DLVAGRPTTVQF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT
Sbjct: 1021 DLVAGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080

Query: 1081 PLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140
            PLN QGDYVNALRAA+EF +KISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA
Sbjct: 1081 PLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140

Query: 1141 IFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200
            IFIVS VITS LWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV
Sbjct: 1141 IFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200

Query: 1201 HLVHAFSVSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYL 1260
            HLVHAFSVSCGDRSQR++EAL+TMGASVFSGITLTKLVGVIVLCFAKSEIFV+YYFQMYL
Sbjct: 1201 HLVHAFSVSCGDRSQRSQEALTTMGASVFSGITLTKLVGVIVLCFAKSEIFVIYYFQMYL 1260

Query: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRSLAIGDVHEETELLVS 1303
            ALVIIGFLHGLVFLPVILSMIGPPSR     D   ETEL VS
Sbjct: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRYSVQDDAPIETELHVS 1301

BLAST of Sed0023022 vs. NCBI nr
Match: KAG6604974.1 (NPC intracellular cholesterol transporter 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2327.4 bits (6030), Expect = 0.0e+00
Identity = 1191/1302 (91.47%), Postives = 1233/1302 (94.70%), Query Frame = 0

Query: 1    MFLNRGVMASRLRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMYD 60
            MFLNRG MA RLRF +SI L QM+FFVTMLIGGEADLSV V S STSEE+HS EYCAMYD
Sbjct: 1    MFLNRGGMACRLRFRISIFLLQMIFFVTMLIGGEADLSVPVRSASTSEERHSEEYCAMYD 60

Query: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120
            ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRS   QAIP
Sbjct: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRS---QAIP 120

Query: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLYD 180
            LFVGCPACLRNFLNLFCELSCSPRQSLFINV+SIAEVGGKMTVDGIDY+VTEKFGKGLYD
Sbjct: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDYFVTEKFGKGLYD 180

Query: 181  SCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQM 240
            SCKDVKFGTMNTRAIDFVGGGAKS EE FAFLGQK V PG+PGSPY+INFK N DK SQM
Sbjct: 181  SCKDVKFGTMNTRAIDFVGGGAKSSEEFFAFLGQK-VGPGIPGSPYSINFKANTDKPSQM 240

Query: 241  ELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITI 300
            ELMNVS YSCGDTSLGCSCGDCP SP CSSLEPP PPK NACTIRIWS KSSCIDFSITI
Sbjct: 241  ELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSLKSSCIDFSITI 300

Query: 301  LYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHL 360
            LYV+FIS FLGWALFHPTRERRRFS+ EEPLLNIGDDG++NSVNLEEN+NG TKEH  HL
Sbjct: 301  LYVIFISAFLGWALFHPTRERRRFSAREEPLLNIGDDGQVNSVNLEENENGATKEHGVHL 360

Query: 361  TLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420
            TL+N VQLSTIQ YI++FYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL
Sbjct: 361  TLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420

Query: 421  WVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNKV 480
            WVGHGS+AA EKQFFD HLAPFYRIEQLIIATKP GR   APRIVTEDNILLLFDIQNKV
Sbjct: 421  WVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDNILLLFDIQNKV 480

Query: 481  NELVANYSGSVVSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYTS 540
            NELVANYSGSVVSL DICLKPL +DCATQSILQY+KMDPENFD+YGGV+H EYC QHYTS
Sbjct: 481  NELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVEHVEYCLQHYTS 540

Query: 541  TETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFI 600
            TETCFSAFKAPLDPSTSLGGFVGNNY+EASAFVVTYPVNNAID VG+EN KAIAWEKAF+
Sbjct: 541  TETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDENGKAIAWEKAFV 600

Query: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSN 660
            KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTI VSYLVMFAYISVALGDSN
Sbjct: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMFAYISVALGDSN 660

Query: 661  ISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMC 720
            ISSSFY+SSKVLLGLSGV+LVVLSVLGS+GFFSA+GVKSTLIIMEVIPFLVLAVGVDNMC
Sbjct: 661  ISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPFLVLAVGVDNMC 720

Query: 721  ILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780
            ILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA
Sbjct: 721  ILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780

Query: 781  LAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSRY 840
            LAV LDFILQLSAFVALIVFDI RAENHRVDCFPCIKVP  SDE NQGFNQ R GLLSRY
Sbjct: 781  LAVFLDFILQLSAFVALIVFDIRRAENHRVDCFPCIKVPPRSDEPNQGFNQRRLGLLSRY 840

Query: 841  MKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLA 900
            MKDVHAPLLGLWGVKIAV++IFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ YFDDLA
Sbjct: 841  MKDVHAPLLGLWGVKIAVVIIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQGYFDDLA 900

Query: 901  EHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPAA 960
            E+LRIGPPLYFVVKDYNYSSKSR+TNQLCSISQCDSNSLLNEISRASLTPEL+YIAKPAA
Sbjct: 901  EYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLTPELNYIAKPAA 960

Query: 961  SWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRHS 1020
            SWLDDFLVWLSPEAFGCCRKFTN SYCPPDDQPPCC PDEG+CD+S GVC+DCTTCF HS
Sbjct: 961  SWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGVCQDCTTCFHHS 1020

Query: 1021 DLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080
            DLVAGRPTTVQF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT
Sbjct: 1021 DLVAGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080

Query: 1081 PLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140
            PLN QGDYVNALRAA+EF +KISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA
Sbjct: 1081 PLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140

Query: 1141 IFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200
            IFIVS VITS LWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV
Sbjct: 1141 IFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200

Query: 1201 HLVHAFSVSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYL 1260
            HLVHAFSVSCGDRSQRAREAL+TMGASVFSGITLTKLVGVIVLCFAKSEIFV+YYFQMYL
Sbjct: 1201 HLVHAFSVSCGDRSQRAREALTTMGASVFSGITLTKLVGVIVLCFAKSEIFVIYYFQMYL 1260

Query: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRSLAIGDVHEETELLVS 1303
            ALVIIGFLHGLVFLPV+LSMIGPPSR L   D   ETEL VS
Sbjct: 1261 ALVIIGFLHGLVFLPVMLSMIGPPSRYLIQDDAPIETELHVS 1298

BLAST of Sed0023022 vs. NCBI nr
Match: XP_038878641.1 (NPC intracellular cholesterol transporter 1-like [Benincasa hispida])

HSP 1 Score: 2319.7 bits (6010), Expect = 0.0e+00
Identity = 1192/1303 (91.48%), Postives = 1230/1303 (94.40%), Query Frame = 0

Query: 1    MFLNR-GVMASRLRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMY 60
            MFL R G MASRLRF +SI L QM+F  +MLIGGEADLSV   S  TS E+H+AEYCAMY
Sbjct: 1    MFLTRGGGMASRLRFSISILLLQMIFLASMLIGGEADLSVHARSGFTSGERHAAEYCAMY 60

Query: 61   DICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAI 120
            DICGARSDGKVLNCPYGSPSVKPDELFS KIQSLCPTISGNVCCTEAQFETLRSQVQQAI
Sbjct: 61   DICGARSDGKVLNCPYGSPSVKPDELFSAKIQSLCPTISGNVCCTEAQFETLRSQVQQAI 120

Query: 121  PLFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLY 180
            PLFVGCPACLRNFLNLFCELSCS RQSLFINV+SIAEVGG MTVDGIDYYVTEKFGKGLY
Sbjct: 121  PLFVGCPACLRNFLNLFCELSCSSRQSLFINVTSIAEVGGSMTVDGIDYYVTEKFGKGLY 180

Query: 181  DSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQ 240
            DSCKDVKFGTMNTRA+DFVGGGAKSFEE FAFLGQKV  PG PGSPYAINFKVN  KSSQ
Sbjct: 181  DSCKDVKFGTMNTRALDFVGGGAKSFEEFFAFLGQKVA-PGFPGSPYAINFKVNPSKSSQ 240

Query: 241  MELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSIT 300
            MELMNVS YSCGDTSLGCSCGDCP SPVCS LE PPPPK NACTI IWS K SCIDFSIT
Sbjct: 241  MELMNVSVYSCGDTSLGCSCGDCPSSPVCSGLE-PPPPKSNACTINIWSLKISCIDFSIT 300

Query: 301  ILYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAH 360
            ILYV+FIS FLGWALFHPTRE RR S+  EPLLNIGDDGEI SVNLEEN+NG TKEH  H
Sbjct: 301  ILYVIFISSFLGWALFHPTRESRRSSARGEPLLNIGDDGEIQSVNLEENENGATKEHGVH 360

Query: 361  LTLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEK 420
            LTL+N VQLSTIQ YISNFYR YGAWVARNPILVLCTSLSIVLILC+GLV FKVETRPEK
Sbjct: 361  LTLRNGVQLSTIQGYISNFYREYGAWVARNPILVLCTSLSIVLILCMGLVCFKVETRPEK 420

Query: 421  LWVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNK 480
            LWVGHGS+AA EKQFFD HLAPFYRIEQLIIATKP GRHGRAPRIVTEDNILLLFDIQNK
Sbjct: 421  LWVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPGGRHGRAPRIVTEDNILLLFDIQNK 480

Query: 481  VNELVANYSGSVVSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYT 540
            VNELVANYSGSVVSLNDICLKPL +DCATQSILQY+KM+PENFDDYGGV+HAEYCFQHYT
Sbjct: 481  VNELVANYSGSVVSLNDICLKPLGEDCATQSILQYFKMNPENFDDYGGVEHAEYCFQHYT 540

Query: 541  STETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAF 600
            STETCFSAFKAPLDPSTSLGGFVGNNY+EASAFVVTYPVNNA+D+VGNEN KAIAWEKAF
Sbjct: 541  STETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAMDSVGNENGKAIAWEKAF 600

Query: 601  IKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDS 660
            +KLAKEELLPLVQS+NLTLSFSSESSIEEELKRESTAD+LTI VSYLVMFAYISVALGDS
Sbjct: 601  VKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTADILTIAVSYLVMFAYISVALGDS 660

Query: 661  NISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNM 720
            NISSSFY+SSKVLLGLSGVILV+LSVLGS+GFFSAIGVKSTLIIMEVIPFLVLAVGVDNM
Sbjct: 661  NISSSFYLSSKVLLGLSGVILVLLSVLGSVGFFSAIGVKSTLIIMEVIPFLVLAVGVDNM 720

Query: 721  CILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFA 780
            CILVHAVKRQP+ELTLEERISSALVEVGPSITLASLSEILAFAVG FVPMPACRVFSMFA
Sbjct: 721  CILVHAVKRQPYELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMPACRVFSMFA 780

Query: 781  ALAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSR 840
            ALAVLLDFILQLSAFVALIVFDILRAE+HRVDCFPCIKVP HSDE NQGFNQGRPGLLSR
Sbjct: 781  ALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVPPHSDEPNQGFNQGRPGLLSR 840

Query: 841  YMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDL 900
            YMKDVHAPLLGLWGVKI V+VIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFD+L
Sbjct: 841  YMKDVHAPLLGLWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDEL 900

Query: 901  AEHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPA 960
            AE+LRIGPP+YFVVKDYNYSSKSR+TNQLCSISQCDSNSLLNEISRASLTPELSYIAKPA
Sbjct: 901  AEYLRIGPPVYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPA 960

Query: 961  ASWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRH 1020
            ASWLDDFLVWLSPEAFGCCRKFTN SYCPPDDQPPCCFPDEG CDSS GVCKDCTTCF H
Sbjct: 961  ASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSEGVCKDCTTCFHH 1020

Query: 1021 SDLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYH 1080
            SDL+  RPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNL GYESGIIKASEFRSYH
Sbjct: 1021 SDLIGDRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYESGIIKASEFRSYH 1080

Query: 1081 TPLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALG 1140
            TPLN QGDYVNALRAA+EFSAKISDSLKMDIFPYSVFYIFFEQYLDIW+TALMNIAIALG
Sbjct: 1081 TPLNKQGDYVNALRAAKEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWETALMNIAIALG 1140

Query: 1141 AIFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFC 1200
            AIFIVS VITSSLWSS IIILVLAMIIIDLMGVMAIL IQLNAVSVVNILMSIGIAVEFC
Sbjct: 1141 AIFIVSLVITSSLWSSAIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFC 1200

Query: 1201 VHLVHAFSVSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMY 1260
            VHLVHAF VSCGDRSQRA++ALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMY
Sbjct: 1201 VHLVHAFLVSCGDRSQRAQQALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMY 1260

Query: 1261 LALVIIGFLHGLVFLPVILSMIGPPSRSLAIGDVHEETELLVS 1303
            LALVIIGFLHGLVFLPVILSMIGPPSR L   D   ETELLVS
Sbjct: 1261 LALVIIGFLHGLVFLPVILSMIGPPSRYLVKDDDPMETELLVS 1301

BLAST of Sed0023022 vs. ExPASy Swiss-Prot
Match: P56941 (NPC intracellular cholesterol transporter 1 OS=Sus scrofa OX=9823 GN=NPC1 PE=2 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 2.8e-204
Identity = 478/1311 (36.46%), Postives = 727/1311 (55.45%), Query Frame = 0

Query: 53   AEYCAMYDICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCP-TISGNV--CCTEAQFE 112
            ++ C  Y  CG  S  K  NC Y  P     E   + +Q LCP    GNV  CC   Q  
Sbjct: 22   SQSCVWYGECGIASGDKRYNCRYSGPPKPLPEDGYDLVQELCPGFFFGNVSLCCDVQQLR 81

Query: 113  TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMT------V 172
            TL+  +Q  +     CP+C  N +NLFCEL+CSPRQS F+NV++  +    +T      V
Sbjct: 82   TLKDNLQLPLQFLSRCPSCFYNLMNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNV 141

Query: 173  DGIDYYVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGG---GAKSFEELFAFLGQKVVDPG 232
              ++YYV E F   +Y++C+DV+  + N +A+  + G    A +      ++  K  D G
Sbjct: 142  KELEYYVGETFANAMYNACRDVEAPSSNEKALGLLCGREAQACNATNWIEYMFNK--DNG 201

Query: 233  LPGSPYAINFKVNADKSSQMELMNVSAYSCGDT----SLGCSCGDCPLSPVCSSLEPPPP 292
               +P+ I    +   +  ME MN +   C ++    +  CSC DC  S VC     PPP
Sbjct: 202  --QAPFTITPIFSDLPTHGMEPMNNATKGCDESVDEVTGPCSCQDC--SIVCGPKPQPPP 261

Query: 293  PK-------RNACTIRIWSFKSSCIDFSITILYVLFISVFLGWALFHPTRERRRFSSGEE 352
            P         +A  + +WS             Y+ F+ VF G A F     R+R+   E 
Sbjct: 262  PPVPWRILGLDAMYVIMWS------------SYMAFLIVFFG-AFFAVWCYRKRYFVSEY 321

Query: 353  PLLNIGDDGEINSVNLEENDNGPTKEHVAHLTLKNDVQLSTIQRYISNFYRNYGAWVARN 412
              +    DG I + ++  +D G             D   +  +R +   +  +GA+  R+
Sbjct: 322  TPI----DGNI-AFSVNSSDKGQ--------AFCCDPLGAAFERGLRRLFAQWGAFCVRH 381

Query: 413  PILVLCTSLSIVLILCVGLVRFKVETRPEKLWVGHGSRAAVEKQFFDRHLAPFYRIEQLI 472
            P  V+  SL+ ++    GLV  +V T P  LW   GS+A  EK++FD H  PF+R+EQLI
Sbjct: 382  PGCVVFFSLAFIVACSSGLVFIRVTTDPVDLWSAPGSQARREKEYFDTHFGPFFRMEQLI 441

Query: 473  IATKPEGRHGRAP----------RIVTEDNILLLFDIQNKVNELVANYSGSVVSLNDICL 532
            I       H   P            ++ D +  + D+Q  +  + A+Y+   V+L DICL
Sbjct: 442  IRATNNQSHIYHPYPAGADVPFGPPLSRDILHQVLDLQTAIENITASYNNETVTLQDICL 501

Query: 533  KPL---DKDCATQSILQYYKMDPENFDDYGG---------VDHAEYCFQHYTST------ 592
             PL   +K+C   S+L Y++      D   G           H  YC +   S       
Sbjct: 502  APLSPYNKNCTILSVLNYFQNSHSVLDHQVGDFFFVYADYHTHFLYCVRAPASLNDASLL 561

Query: 593  -ETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFI 652
             + C   F  P+ P   LGG+   NY  A+A V+T+PVNN  +    + ++A AWE  FI
Sbjct: 562  HDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDT-EKLQRAQAWESEFI 621

Query: 653  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSN 712
               K       ++ NLT+SF +E SIE+EL RES +D+ TI++SY +MF YIS+ALG   
Sbjct: 622  NFVKN-----YKNPNLTISFMAERSIEDELNRESNSDLFTILISYAIMFLYISIALGHIK 681

Query: 713  ISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMC 772
              S   + SK+ LG++G+++V+ SV  S+G FS IGV  TLI++EVIPFLVLAVGVDN+ 
Sbjct: 682  SCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYIGVPLTLIVIEVIPFLVLAVGVDNIF 741

Query: 773  ILVHAVKRQP--FELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMF 832
            ILV   +R       TL++++   L EV PS+ L+S SE +AF +G    +PA   FS+F
Sbjct: 742  ILVQTYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGGLSVVPAVHTFSLF 801

Query: 833  AALAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLS 892
            A +AVL+DF+LQ++ FV+L+  DI R E +R+D   C++      E   G  Q     L 
Sbjct: 802  AGMAVLIDFLLQITCFVSLLGLDIKRQEKNRLDVVCCVQ----GAEDGAGV-QASESCLF 861

Query: 893  RYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDD 952
            R+ K+ +APLL    ++  VI +FVG+   SIA+  K+E+GL+Q + +P DSY+ DYF  
Sbjct: 862  RFFKNSYAPLLLKDWMRPIVIAVFVGVLSFSIAVLNKVEIGLDQSLSMPDDSYVMDYFQS 921

Query: 953  LAEHLRIGPPLYFVVKD-YNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAK 1012
            L+ +L  GPP+YFVV++ +NY+S  +  N +C    C+++SL+ +I  A+     + I  
Sbjct: 922  LSRYLHAGPPVYFVVEEGHNYTS-LKGQNMVCGGLGCNNDSLVQQIFTAAQLDNYTRIGF 981

Query: 1013 PAASWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCF 1072
              +SW+DD+  W+ P++  CCR + ++               + +C++S  V   C  C 
Sbjct: 982  APSSWIDDYFDWIKPQS-SCCRVYNST---------------DQFCNASV-VDPTCIRCR 1041

Query: 1073 RHSDLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRS 1132
              +     RP    F   LP FL+  P+  C KGGH AY+++VN+ G  SG + A+ F +
Sbjct: 1042 PLTSEGKQRPQGEDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILGNGSG-VGATYFMT 1101

Query: 1133 YHTPLNNQGDYVNALRAAQEFSAKISDSLKMD-----IFPYSVFYIFFEQYLDIWKTALM 1192
            YHT L    D+++A++ A+  ++ I+ ++ ++     +FPYSVFY+F+EQYL +    + 
Sbjct: 1102 YHTVLQASADFIDAMQKARLIASNITRTMGLEASSYRVFPYSVFYVFYEQYLTVIDDTIF 1161

Query: 1193 NIAIALGAIFIVSFVITS-SLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMS 1252
            N+ ++LGAIF+V+ V+    LW++ I+ + +AMI++++ GVM +  I LNAVS+VN++MS
Sbjct: 1162 NLGVSLGAIFLVTVVLMGCELWATVIMCVTIAMILVNMFGVMWLWGISLNAVSLVNLVMS 1221

Query: 1253 IGIAVEFCVHLVHAFSVSC-GDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEI 1299
             GI+VEFC H+  AF++S  G R  RA EAL+ MG+SVFSGITLTK  G++VL FAKS+I
Sbjct: 1222 CGISVEFCSHITRAFTLSTKGSRVDRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQI 1270

BLAST of Sed0023022 vs. ExPASy Swiss-Prot
Match: O35604 (NPC intracellular cholesterol transporter 1 OS=Mus musculus OX=10090 GN=Npc1 PE=1 SV=2)

HSP 1 Score: 711.1 bits (1834), Expect = 2.4e-203
Identity = 465/1288 (36.10%), Postives = 713/1288 (55.36%), Query Frame = 0

Query: 53   AEYCAMYDICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTI---SGNVCCTEAQFE 112
            ++ C  Y  CG  +  K  NC Y  P     +   + +Q LCP +   + ++CC   Q +
Sbjct: 22   SQSCVWYGECGIATGDKRYNCKYSGPPKPLPKDGYDLVQELCPGLFFDNVSLCCDIQQLQ 81

Query: 113  TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEV------GGKMTV 172
            TL+S +Q  +     CP+C  N + LFCEL+CSP QS F+NV++  +         K  V
Sbjct: 82   TLKSNLQLPLQFLSRCPSCFYNLMTLFCELTCSPHQSQFLNVTATEDYFDPKTQENKTNV 141

Query: 173  DGIDYYVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGG---GAKSFEELFAFLGQKVVDPG 232
              ++Y+V + F   +Y++C+DV+  + N +A+  + G    A +      ++  K  D G
Sbjct: 142  KELEYFVGQSFANAMYNACRDVEAPSSNEKALGLLCGRDARACNATNWIEYMFNK--DNG 201

Query: 233  LPGSPYAINFKVNADKS-SQMELMNVSAYSCGDT----SLGCSCGDCPLSPVCSSLEPPP 292
               +P+ I   V +D S   ME M  +   C ++    +  CSC DC  S VC     PP
Sbjct: 202  --QAPFTI-IPVFSDLSILGMEPMRNATKGCNESVDEVTGPCSCQDC--SIVCGPKPQPP 261

Query: 293  PPKRNACTIRIWSFKSSCIDFSITILYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIG 352
            PP       RIW   +  +   +T  YV F+ VF G  L      RR F S   P+    
Sbjct: 262  PP---PMPWRIWGLDAMYVIMWVT--YVAFLFVFFGALLAVWCHRRRYFVSEYTPI---- 321

Query: 353  DDGEINSVNLEENDNGPTKEHVAHLTLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLC 412
            D     SVN  +       + +           +     +   +  +GA+  RNP  ++ 
Sbjct: 322  DSNIAFSVNSSDKGEASCCDPLG----------AAFDDCLRRMFTKWGAFCVRNPTCIIF 381

Query: 413  TSLSIVLILCVGLVRFKVETRPEKLWVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPE 472
             SL+ + +   GLV  +V T P +LW    S+A +EK++FD+H  PF+R EQLII     
Sbjct: 382  FSLAFITVCSSGLVFVQVTTNPVELWSAPHSQARLEKEYFDKHFGPFFRTEQLIIQAPNT 441

Query: 473  GRHGRAP----------RIVTEDNILLLFDIQNKVNELVANYSGSVVSLNDICLKPL--- 532
              H   P            + ++ +  + D+Q  +  + A+Y+   V+L DIC+ PL   
Sbjct: 442  SVHIYEPYPAGADVPFGPPLNKEILHQVLDLQIAIESITASYNNETVTLQDICVAPLSPY 501

Query: 533  DKDCATQSILQYYKMDPENFDDYGGVD---------HAEYCFQHYTSTE-------TCFS 592
            +K+C   S+L Y++      D   G D         H  YC +   S          C  
Sbjct: 502  NKNCTIMSVLNYFQNSHAVLDSQVGDDFYIYADYHTHFLYCVRAPASLNDTSLLHGPCLG 561

Query: 593  AFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFIKLAKEE 652
             F  P+ P   LGG+   NY  A+A V+T+PVNN  +      ++A AWEK FI   K  
Sbjct: 562  TFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDT-ERLQRAWAWEKEFISFVKN- 621

Query: 653  LLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSNISSSFY 712
                 ++ NLT+SF++E SIE+EL RES +DV T+++SY+VMF YIS+ALG     S   
Sbjct: 622  ----YKNPNLTISFTAERSIEDELNRESNSDVFTVIISYVVMFLYISLALGHIQSCSRLL 681

Query: 713  ISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCILVHAV 772
            + SK+ LG++G+++V+ SV  S+G FS +G+  TLI++EVIPFLVLAVGVDN+ ILV   
Sbjct: 682  VDSKISLGIAGILIVLSSVACSLGIFSYMGMPLTLIVIEVIPFLVLAVGVDNIFILVQTY 741

Query: 773  KRQP--FELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAALAVL 832
            +R     E TL++++   L EV P++ L+S SE  AF  G    MPA   FS+FA +AVL
Sbjct: 742  QRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPAVHTFSLFAGMAVL 801

Query: 833  LDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSRYMKDV 892
            +DF+LQ++ FV+L+  DI R E + +D   C++      +  QG +      L R+ K+ 
Sbjct: 802  IDFLLQITCFVSLLGLDIKRQEKNHLDILCCVR----GADDGQG-SHASESYLFRFFKNY 861

Query: 893  HAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLAEHLR 952
             APLL    ++  V+ +FVG+   S+A+  K+++GL+Q + +P DSY+ DYF  LA++L 
Sbjct: 862  FAPLLLKDWLRPIVVAVFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYVIDYFKSLAQYLH 921

Query: 953  IGPPLYFVVKD-YNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPAASWL 1012
             GPP+YFV+++ YNYSS+ +  N +C    CD++SL+ +I  A+     + +    +SW+
Sbjct: 922  SGPPVYFVLEEGYNYSSR-KGQNMVCGGMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWI 981

Query: 1013 DDFLVWLSPEAFGCCRKF-TNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRHSDL 1072
            DD+  W+SP++  CCR +     +C      P C             C+  T   +    
Sbjct: 982  DDYFDWVSPQS-SCCRLYNVTHQFCNASVMDPTCVR-----------CRPLTPEGKQ--- 1041

Query: 1073 VAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPL 1132
               RP   +F + LP FL+  P+  C KGGH AY ++VN+ G +   I A+ F +YHT L
Sbjct: 1042 ---RPQGKEFMKFLPMFLSDNPNPKCGKGGHAAYGSAVNIVG-DDTYIGATYFMTYHTIL 1101

Query: 1133 NNQGDYVNALRAAQEFSAKISDSLKMD-----IFPYSVFYIFFEQYLDIWKTALMNIAIA 1192
                DY +A++ A+  ++ I+++++       +FPYSVFY+F+EQYL I    + N++++
Sbjct: 1102 KTSADYTDAMKKARLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDTIFNLSVS 1161

Query: 1193 LGAIFIVSFVITS-SLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAV 1252
            LG+IF+V+ V+    LWS+ I+ + +AMI++++ GVM +  I LNAVS+VN++MS GI+V
Sbjct: 1162 LGSIFLVTLVVLGCELWSAVIMCITIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISV 1221

Query: 1253 EFCVHLVHAFSVSC-GDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYY 1284
            EFC H+  AF++S  G R  RA EAL+ MG+SVFSGITLTK  G++VL FAKS+IF ++Y
Sbjct: 1222 EFCSHITRAFTMSTKGSRVSRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFY 1252

BLAST of Sed0023022 vs. ExPASy Swiss-Prot
Match: O15118 (NPC intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC1 PE=1 SV=2)

HSP 1 Score: 706.4 bits (1822), Expect = 5.8e-202
Identity = 470/1304 (36.04%), Postives = 713/1304 (54.68%), Query Frame = 0

Query: 53   AEYCAMYDICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCP-TISGNV--CCTEAQFE 112
            ++ C  Y  CG     K  NC Y  P     +   + +Q LCP    GNV  CC   Q +
Sbjct: 22   SQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQ 81

Query: 113  TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMT------V 172
            TL+  +Q  +     CP+C  N LNLFCEL+CSPRQS F+NV++  +    +T      V
Sbjct: 82   TLKDNLQLPLQFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNV 141

Query: 173  DGIDYYVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGG---GAKSFEELFAFLGQKVVDPG 232
              + YYV + F   +Y++C+DV+  + N +A+  + G    A +      ++  K  D G
Sbjct: 142  KELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNK--DNG 201

Query: 233  LPGSPYAINFKVNADKSSQMELMNVSAYSCGDT----SLGCSCGDCPLSPVCSSLEPPPP 292
               +P+ I    +      ME MN +   C ++    +  CSC DC  S VC     PPP
Sbjct: 202  --QAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDC--SIVCGPKPQPPP 261

Query: 293  PKRNACTIRIWSFKSSCIDFSIT-ILYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIG 352
            P         W+       + I  I Y+ F+ VF G A F     R+R+   E   +   
Sbjct: 262  PPAP------WTILGLDAMYVIMWITYMAFLLVFFG-AFFAVWCYRKRYFVSEYTPI--- 321

Query: 353  DDGEINSVNLEENDNGPTKEHVAHLTLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLC 412
            D     SVN  +       + V+          +  +  +   +  +G++  RNP  V+ 
Sbjct: 322  DSNIAFSVNASDKGEASCCDPVS----------AAFEGCLRRLFTRWGSFCVRNPGCVIF 381

Query: 413  TSLSIVLILCVGLVRFKVETRPEKLWVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPE 472
             SL  +     GLV  +V T P  LW    S+A +EK++FD+H  PF+R EQLII     
Sbjct: 382  FSLVFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLT 441

Query: 473  GRHGRAPRIVTED-------NILLL---FDIQNKVNELVANYSGSVVSLNDICLKPL--- 532
             +H   P     D       +I +L    D+Q  +  + A+Y    V+L DICL PL   
Sbjct: 442  DKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPY 501

Query: 533  DKDCATQSILQYYKMDPENFDDYGGVD---------HAEYCFQHYTST-------ETCFS 592
            + +C   S+L Y++      D   G D         H  YC +   S        + C  
Sbjct: 502  NTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLG 561

Query: 593  AFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFIKLAKEE 652
             F  P+ P   LGG+   NY  A+A V+T+PVNN  +    + ++A AWEK FI   K  
Sbjct: 562  TFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDT-EKLQRAQAWEKEFINFVKN- 621

Query: 653  LLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSNISSSFY 712
                 ++ NLT+SF++E SIE+EL RES +DV T+V+SY +MF YIS+ALG         
Sbjct: 622  ----YKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLL 681

Query: 713  ISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCILVHAV 772
            + SKV LG++G+++V+ SV  S+G FS IG+  TLI++EVIPFLVLAVGVDN+ ILV A 
Sbjct: 682  VDSKVSLGIAGILIVLSSVACSLGVFSYIGLPLTLIVIEVIPFLVLAVGVDNIFILVQAY 741

Query: 773  KRQP--FELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAALAVL 832
            +R       TL++++   L EV PS+ L+S SE +AF +G    MPA   FS+FA LAV 
Sbjct: 742  QRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPAVHTFSLFAGLAVF 801

Query: 833  LDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSRYMKDV 892
            +DF+LQ++ FV+L+  DI R E +R+D F C++             Q     L R+ K+ 
Sbjct: 802  IDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAEDGTS-----VQASESCLFRFFKNS 861

Query: 893  HAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLAEHLR 952
            ++PLL    ++  VI IFVG+   SIA+  K+++GL+Q + +P DSY+ DYF  ++++L 
Sbjct: 862  YSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLH 921

Query: 953  IGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPAASWLD 1012
             GPP+YFV+++ +  + S+  N +C    C+++SL+ +I  A+     + I    +SW+D
Sbjct: 922  AGPPVYFVLEEGHDYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWID 981

Query: 1013 DFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRHSDLVA 1072
            D+  W+ P++  CCR    +               + +C++S  V   C  C   +    
Sbjct: 982  DYFDWVKPQS-SCCRVDNIT---------------DQFCNASV-VDPACVRCRPLTPEGK 1041

Query: 1073 GRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNN 1132
             RP    F   LP FL+  P+  C KGGH AY+++VN+       + A+ F +YHT L  
Sbjct: 1042 QRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVGATYFMTYHTVLQT 1101

Query: 1133 QGDYVNALRAAQEFSAKISDSLKMD-----IFPYSVFYIFFEQYLDIWKTALMNIAIALG 1192
              D+++AL+ A+  ++ +++++ ++     +FPYSVFY+F+EQYL I    + N+ ++LG
Sbjct: 1102 SADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLG 1161

Query: 1193 AIFIVSFVITS-SLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEF 1252
            AIF+V+ V+    LWS+ I+   +AM+++++ GVM +  I LNAVS+VN++MS GI+VEF
Sbjct: 1162 AIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEF 1221

Query: 1253 CVHLVHAFSVSC-GDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQ 1299
            C H+  AF+VS  G R +RA EAL+ MG+SVFSGITLTK  G++VL FAKS+IF ++YF+
Sbjct: 1222 CSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFR 1271

BLAST of Sed0023022 vs. ExPASy Swiss-Prot
Match: Q6T3U3 (NPC1-like intracellular cholesterol transporter 1 OS=Rattus norvegicus OX=10116 GN=Npc1l1 PE=1 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 3.0e-166
Identity = 429/1323 (32.43%), Postives = 677/1323 (51.17%), Query Frame = 0

Query: 50   KHSAEYCAMYDICGAR---SDGKV----LNCPYGSPSVKPDELFSEKIQSLCPTISGN-- 109
            KH A  C  Y+ CG     S G      ++C   +P+          +Q +CP +     
Sbjct: 26   KHEAGVCTFYEECGKNPELSGGLTSLSNVSCLSNTPARHVTGEHLALLQRICPRLYNGPN 85

Query: 110  ---VCCTEAQFETLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAE- 169
                CC+  Q  +L S +     L   CPAC  NF++L C  +CSP QSLFINV+ + E 
Sbjct: 86   TTFACCSTKQLLSLESSMSITKALLTRCPACSDNFVSLHCHNTCSPDQSLFINVTRVVER 145

Query: 170  -VGGKMTVDGIDYYVTEKFGKGLYDSCKDVKFGTMNTRAIDFV----GGGAKSFEELFAF 229
              G    V   + +    F +  Y+SC  V+     + A+  +    G    + +    F
Sbjct: 146  GAGEPPAVVAYEAFYQRSFAEKAYESCSQVRIPAAASLAVGSMCGVYGSALCNAQRWLNF 205

Query: 230  LGQ------------KVVDPG--LPGSPYAINFKVNADKSSQMELMNVSAYSCGDTSLGC 289
             G              +++PG  LP     +N K+     SQ           GD S  C
Sbjct: 206  QGDTGNGLAPLDITFHLLEPGQALPDGIQPLNGKIAPCNESQ-----------GDDSAVC 265

Query: 290  SCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITILYVLFISVFLGWALFHP 349
            SC DC  S  C  + PP   + +    R+  + +  I F  T ++VL  +V +   +   
Sbjct: 266  SCQDCAAS--CPVIPPPEALRPSFYMGRMPGWLALIIIF--TAVFVLLSAVLVRLRVVSN 325

Query: 350  TRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHLTLKNDVQLSTIQRYISN 409
              + +     E P L                   P K  ++  T+            +  
Sbjct: 326  RNKNKAEGPQEAPKL-------------------PHKHKLSPHTI------------LGR 385

Query: 410  FYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKLWVGHGSRAAVEKQFFDR 469
            F++N+G  VA  P+ VL  S  +V+ L  GL   ++ T P +LW    S+A  EK F D 
Sbjct: 386  FFQNWGTRVASWPLTVLALSFIVVIALAAGLTFIELTTDPVELWSAPKSQARKEKSFHDE 445

Query: 470  HLAPFYRIEQLIIATKPEGRH---------GRAPRIVTEDNILLLFDIQNKVNELV--AN 529
            H  PF+R  Q+ +  +    +              I++ D +L L ++Q ++  L   + 
Sbjct: 446  HFGPFFRTNQIFVTARNRSSYKYDSLLLGSKNFSGILSLDFLLELLELQERLRHLQVWSP 505

Query: 530  YSGSVVSLNDICLKPLD------KDCATQSILQYYK------MDPENFDDYGGV------ 589
             +   +SL DIC  PL+       DC   S+LQY++      M   N    G        
Sbjct: 506  EAERNISLQDICYAPLNPYNTSLSDCCVNSLLQYFQNNRTLLMLTANQTLNGQTSLVDWK 565

Query: 590  DHAEYC------FQHYTSTE-TCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNA 649
            DH  YC      F+  TS   +C + + AP+ P  ++GG+ G +Y+EA A ++T+ +NN 
Sbjct: 566  DHFLYCANAPLTFKDGTSLALSCMADYGAPVFPFLAVGGYQGTDYSEAEALIITFSLNN- 625

Query: 650  IDAVGNENEKAIAWEKAFIKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTI 709
              A      +A  WE+AF+K  + E      S    ++FS+E S+E+E+ R +  D+   
Sbjct: 626  YPADDPRMAQAKLWEEAFLK--EMESFQRNTSDKFQVAFSAERSLEDEINRTTIQDLPVF 685

Query: 710  VVSYLVMFAYISVALGDSNISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTL 769
             VSY+++F YIS+ALG  +  S   + SK  LGL GVI+V+ +VL ++GF+S +GV S+L
Sbjct: 686  AVSYIIVFLYISLALGSYSRCSRVAVESKATLGLGGVIVVLGAVLAAMGFYSYLGVPSSL 745

Query: 770  IIMEVIPFLVLAVGVDNMCILVHAVKRQPFELTLEER---ISSALVEVGPSITLASLSEI 829
            +I++V+PFLVLAVG DN+ I V   +R P  +  E+R   I   L  V PS+ L SLSE 
Sbjct: 746  VIIQVVPFLVLAVGADNIFIFVLEYQRLP-RMPGEQREAHIGRTLGSVAPSMLLCSLSEA 805

Query: 830  LAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAENHRVD---CFPC 889
            + F +G   PMPA R F++ + LA++LDF+LQ++AFVAL+  D  R E  R D   CF  
Sbjct: 806  ICFFLGALTPMPAVRTFALTSGLAIILDFLLQMTAFVALLSLDSKRQEASRPDVLCCFST 865

Query: 890  IKVPSHSDERNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTK 949
             K+P   +         + GLL R+ + ++AP L    ++  V+++F+ L   ++ L   
Sbjct: 866  RKLPPPKE---------KEGLLLRFFRKIYAPFLLHRFIRPVVMLLFLTLFGANLYLMCN 925

Query: 950  IEVGLEQKIVLPRDSYLQDYFDDLAEHLRIGPPLYFV-VKDYNYSSKSRNTNQLCSISQC 1009
            I VGL+Q++ LP+DSYL DYF  L  +L +GPP+YFV    +N+SS++   N  CS + C
Sbjct: 926  INVGLDQELALPKDSYLIDYFLFLNRYLEVGPPVYFVTTSGFNFSSEA-GMNATCSSAGC 985

Query: 1010 DSNSLLNEISRASLTPELSYIAKPAASWLDDFLVWLSPEAFGCCRKFT----NSSYCPPD 1069
             S SL  +I  AS  P+ SY+A  A+SW+DDF+ WL+P +  CCR +        +CP  
Sbjct: 986  KSFSLTQKIQYASEFPDQSYVAIAASSWVDDFIDWLTPSS-SCCRLYIRGPHKDEFCPS- 1045

Query: 1070 DQPPCCFPDEGYCDSSAGVCKDCTTCFRHSDLVAGRPTTVQFQEKLPWFLNSLPSADCAK 1129
                         D+S    K+C     +  L   RPT  QF + LPWFLN  P+  C K
Sbjct: 1046 ------------TDTSFNCLKNC----MNRTLGPVRPTAEQFHKYLPWFLNDPPNIRCPK 1105

Query: 1130 GGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNNQGDYVNALRAAQEFSAKISDSLK--- 1189
            GG  AY  SVNL+    G + AS+F +YH PL N  D+  ALRA++  +A I+  L+   
Sbjct: 1106 GGLAAYRTSVNLS--SDGQVIASQFMAYHKPLRNSQDFTEALRASRLLAANITADLRKVP 1165

Query: 1190 -----MDIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSFVITSSLWSSGII-ILV 1249
                  ++FPY++  +F++QYL +    +  +A+     F+V +++      SGI+ +L 
Sbjct: 1166 GTDPNFEVFPYTISNVFYQQYLTVLPEGIFTLALCFVPTFVVCYLLLGLDMCSGILNLLS 1225

Query: 1250 LAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSC-GDRSQRAREA 1284
            + MI++D +G+MA+  I  NAVS++N++ ++G++VEF  H+  +F+VS    R +RA++A
Sbjct: 1226 IIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSFAVSTKPTRLERAKDA 1268

BLAST of Sed0023022 vs. ExPASy Swiss-Prot
Match: Q9UHC9 (NPC1-like intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC1L1 PE=1 SV=2)

HSP 1 Score: 573.2 bits (1476), Expect = 7.7e-162
Identity = 433/1333 (32.48%), Postives = 676/1333 (50.71%), Query Frame = 0

Query: 51   HSAEYCAMYDICGARSD--GKVL-----NCPYGSPSVKPDELFSEKIQSLCPTI----SG 110
            H   YCA YD CG   +  G ++     +C   +P+ K        +Q +CP +    + 
Sbjct: 28   HQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNT 87

Query: 111  NVCCTEAQFETLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVG- 170
              CC+  Q  +L + +     L   CPAC  NF+NL C  +CSP QSLFINV+ +A++G 
Sbjct: 88   QACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGA 147

Query: 171  GKM-TVDGIDYYVTEKFGKGLYDSCKDVKFGTMNTRAIDFV----GGGAKSFEELFAFLG 230
            G++  V   + +    F +  YDSC  V+     T A+  +    G    + +    F G
Sbjct: 148  GQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQG 207

Query: 231  QKVVDPGLPGSPYAINFKV---NADKSSQMELMNVSAYSC----GDTSLGCSCGDCPLSP 290
                D G   +P  I F +        S ++ +N     C    GD    CSC DC  S 
Sbjct: 208  ----DTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAAS- 267

Query: 291  VCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITILYVLFISVFLGWALFHPTRERRRFSS 350
             C ++     P+    T  +     S +   I       +++ L      P R++ +   
Sbjct: 268  -CPAI---ARPQALDSTFYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVD 327

Query: 351  GEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHLTLKNDVQLSTIQRYISNFYRNYGAWV 410
                                     P K      +L + +  ST    +  F++ +G WV
Sbjct: 328  -------------------------PKK----GTSLSDKLSFST-HTLLGQFFQGWGTWV 387

Query: 411  ARNPILVLCTSLSIVLILCVGLVRFKVETRPEKLWVGHGSRAAVEKQFFDRHLAPFYRIE 470
            A  P+ +L  S+  V+ L  GLV  ++ T P +LW    S+A  EK F D+H  PF+R  
Sbjct: 388  ASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTN 447

Query: 471  QLIIATKPEGRHGR------APR----IVTEDNILLLFDIQNKVNELV--ANYSGSVVSL 530
            Q+I+ T P     R       P+    I+  D +L L ++Q ++  L   +  +   +SL
Sbjct: 448  QVIL-TAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISL 507

Query: 531  NDICLKPLDK------DCATQSILQYYKMDPE------------NFDDYGGVDHAEYC-- 590
             DIC  PL+       DC   S+LQY++ +                      DH  YC  
Sbjct: 508  QDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCAN 567

Query: 591  ----FQHYTSTE-TCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNEN 650
                F+  T+   +C + + AP+ P  ++GG+ G +Y+EA A ++T+ +NN   A     
Sbjct: 568  APLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNN-YPAGDPRL 627

Query: 651  EKAIAWEKAFIKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMF 710
             +A  WE+AF  L +        +    ++F +E S+E+E+ R +  D+     SY+V+F
Sbjct: 628  AQAKLWEEAF--LEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAEDLPIFATSYIVIF 687

Query: 711  AYISVALGDSNISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPF 770
             YIS+ALG  +  S   + SK  LGL GV +V+ +V+ ++GFFS +G++S+L+I++V+PF
Sbjct: 688  LYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVVPF 747

Query: 771  LVLAVGVDNMCILV---HAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTF 830
            LVL+VG DN+ I V     + R+P E   E  I  AL  V PS+ L SLSE + F +G  
Sbjct: 748  LVLSVGADNIFIFVLEYQRLPRRPGE-PREVHIGRALGRVAPSMLLCSLSEAICFFLGAL 807

Query: 831  VPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERN 890
             PMPA R F++ + LAV+LDF+LQ+SAFVAL+  D  R E  R+D   C+K P       
Sbjct: 808  TPMPAVRTFALTSGLAVILDFLLQMSAFVALLSLDSKRQEASRLDVCCCVK-PQELPPPG 867

Query: 891  QGFNQGRPGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVL 950
            QG      GLL  + +  +AP L  W  +  V+++F+ L   S+     I VGL+Q++ L
Sbjct: 868  QG-----EGLLLGFFQKAYAPFLLHWITRGVVLLLFLALFGVSLYSMCHISVGLDQELAL 927

Query: 951  PRDSYLQDYFDDLAEHLRIGPPLYFVVK-DYNYSSKSRNTNQLCSISQCDSNSLLNEISR 1010
            P+DSYL DYF  L  +  +G P+YFV    YN+SS++   N +CS + C++ S   +I  
Sbjct: 928  PKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEA-GMNAICSSAGCNNFSFTQKIQY 987

Query: 1011 ASLTPELSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDS 1070
            A+  PE SY+A PA+SW+DDF+ WL+P +  CCR + +    P  D+         +C S
Sbjct: 988  ATEFPEQSYLAIPASSWVDDFIDWLTPSS--CCRLYISG---PNKDK---------FCPS 1047

Query: 1071 SAGVCKDCTTCFRHSDLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNG- 1130
            +         C   + + + RP+  QF + LPWFLN  P+  C KGG  AY+ SVNL   
Sbjct: 1048 TVNSLNCLKNCMSIT-MGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLAAYSTSVNLTSD 1107

Query: 1131 --------------YESGIIKA----------SEFRSYHTPLNNQGDYVNALRAAQEFSA 1190
                            SG I A          S F +YH PL N  DY  ALRAA+E +A
Sbjct: 1108 GQVLDTVAILSPRLEYSGTISAHCNLYLLDSTSRFMAYHKPLKNSQDYTEALRAARELAA 1167

Query: 1191 KISDSLK--------MDIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSFVITSSL 1250
             I+  L+         ++FPY++  +F+EQYL I    L  +++ L   F VS ++    
Sbjct: 1168 NITADLRKVPGTDPAFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLLGLD 1227

Query: 1251 WSSGII-ILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCG 1284
              SG++ +L + MI++D +G MA+  I  NAVS++N++ ++G++VEF  H+  +F++S  
Sbjct: 1228 LRSGLLNLLSIVMILVDTVGFMALWGISYNAVSLINLVSAVGMSVEFVSHITRSFAISTK 1287

BLAST of Sed0023022 vs. ExPASy TrEMBL
Match: A0A6J1G6Q4 (Niemann-Pick C1 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111451262 PE=3 SV=1)

HSP 1 Score: 2338.5 bits (6059), Expect = 0.0e+00
Identity = 1195/1302 (91.78%), Postives = 1235/1302 (94.85%), Query Frame = 0

Query: 1    MFLNRGVMASRLRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMYD 60
            MFLNRG MA RLRF +SI L QM+FFVTMLIGGEADLSV V S STSEE+HS EYCAMYD
Sbjct: 1    MFLNRGGMACRLRFRISIFLLQMIFFVTMLIGGEADLSVPVRSASTSEERHSEEYCAMYD 60

Query: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120
            ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP
Sbjct: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120

Query: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLYD 180
            LFVGCPACLRNFLNLFCELSCSPRQSLFINV+SIAEVGGKMTVDGIDY+VTEKFGKGLYD
Sbjct: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDYFVTEKFGKGLYD 180

Query: 181  SCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQM 240
            SCKDVKFGTMNTRAIDFVGGGAKS EE FAFLGQK V PG+PGSPY+INFK N DK SQM
Sbjct: 181  SCKDVKFGTMNTRAIDFVGGGAKSSEEFFAFLGQK-VGPGIPGSPYSINFKANTDKPSQM 240

Query: 241  ELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITI 300
            ELMNVS YSCGDTSLGCSCGDCP SP CSSLEPP PPK NACTIRIWS KSSCIDFSITI
Sbjct: 241  ELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSLKSSCIDFSITI 300

Query: 301  LYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHL 360
            LYV+FIS FLGWALFHPTRERRRFS+ EEPLLNIGDDGE+NSVNLEEN+NG TKEH  HL
Sbjct: 301  LYVIFISAFLGWALFHPTRERRRFSAREEPLLNIGDDGEVNSVNLEENENGATKEHGVHL 360

Query: 361  TLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420
            TL+N VQLSTIQ YI++FYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL
Sbjct: 361  TLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420

Query: 421  WVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNKV 480
            WVGHGS+AA EKQFFD HLAPFYRIEQLIIATKP GR   APRIVTEDNILLLFDIQNKV
Sbjct: 421  WVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDNILLLFDIQNKV 480

Query: 481  NELVANYSGSVVSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYTS 540
            NELVANYSGSVVSL DICLKPL +DCATQSILQY+KMDPENFD+YGGV+H EYC QHYTS
Sbjct: 481  NELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVEHVEYCLQHYTS 540

Query: 541  TETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFI 600
            TETCFSAFKAPLDPSTSLGGFVGNNY+EASAFVVTYPVNNAID VG+EN KAIAWEKAF+
Sbjct: 541  TETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDENGKAIAWEKAFV 600

Query: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSN 660
            KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTI VSYLVMFAYISVALGDSN
Sbjct: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMFAYISVALGDSN 660

Query: 661  ISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMC 720
            ISSSFY+SSKVLLGLSGV+LVVLSVLGS+GFFSA+GVKSTLIIMEVIPFLVLAVGVDNMC
Sbjct: 661  ISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPFLVLAVGVDNMC 720

Query: 721  ILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780
            ILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA
Sbjct: 721  ILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780

Query: 781  LAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSRY 840
            LAV LDFILQLSAFVALIVFDI RAENHRVDCFPCIKVP  SDE NQGFNQ R GLLSRY
Sbjct: 781  LAVFLDFILQLSAFVALIVFDIRRAENHRVDCFPCIKVPPRSDEPNQGFNQRRLGLLSRY 840

Query: 841  MKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLA 900
            MKDVHAPLLGLWGVKIAV++IFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ YFDDLA
Sbjct: 841  MKDVHAPLLGLWGVKIAVVIIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQGYFDDLA 900

Query: 901  EHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPAA 960
            E+LRIGPPLYFVVKDYNYSSKSR+TNQLCSISQCDSNSLLNEISRASLTPEL+YIAKPAA
Sbjct: 901  EYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLTPELNYIAKPAA 960

Query: 961  SWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRHS 1020
            SWLDDFLVWLSPEAFGCCRKFTN SYCPPDDQPPCC PDEG+CD+S GVC+DCTTCF HS
Sbjct: 961  SWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGVCQDCTTCFHHS 1020

Query: 1021 DLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080
            DLVAGRPTTVQF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT
Sbjct: 1021 DLVAGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080

Query: 1081 PLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140
            PLN QGDYVNALRAA+EF +KISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA
Sbjct: 1081 PLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140

Query: 1141 IFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200
            IFIVS VITS LWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV
Sbjct: 1141 IFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200

Query: 1201 HLVHAFSVSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYL 1260
            HLVHAFSVSCGDRSQRAREAL+TMGASVFSGITLTKLVGVIVLCFAKSEIFV+YYFQMYL
Sbjct: 1201 HLVHAFSVSCGDRSQRAREALTTMGASVFSGITLTKLVGVIVLCFAKSEIFVIYYFQMYL 1260

Query: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRSLAIGDVHEETELLVS 1303
            ALVIIGFLHGLVFLPVILSMIGPPSR     D   ETEL VS
Sbjct: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRYSVQDDAPIETELHVS 1301

BLAST of Sed0023022 vs. ExPASy TrEMBL
Match: A0A6J1I473 (Niemann-Pick C1 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111469740 PE=3 SV=1)

HSP 1 Score: 2332.4 bits (6043), Expect = 0.0e+00
Identity = 1190/1302 (91.40%), Postives = 1234/1302 (94.78%), Query Frame = 0

Query: 1    MFLNRGVMASRLRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMYD 60
            MFLNR  MA RLRF +SI L QM+FFVTMLIGGEADLSV V S STSEE+HS EYCAMYD
Sbjct: 1    MFLNRRGMACRLRFRISIFLMQMIFFVTMLIGGEADLSVPVRSASTSEERHSEEYCAMYD 60

Query: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120
            ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP
Sbjct: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120

Query: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLYD 180
            LFVGCPACLRNFLNLFCELSCSPRQSLFINV+SIAEVGGKMTVDGIDY+VTEKFGKGLYD
Sbjct: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDYFVTEKFGKGLYD 180

Query: 181  SCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQM 240
            SCKDVKFGTMNTRAIDFVGGGAKSFEE FAFLGQK V PG+PGSPY+INFKVN DK SQ+
Sbjct: 181  SCKDVKFGTMNTRAIDFVGGGAKSFEEFFAFLGQK-VGPGIPGSPYSINFKVNTDKPSQV 240

Query: 241  ELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITI 300
            ELMNVS YSCGDTSLGCSCGDCP SP CSSLEPP PPK NACTIRIWS KSSCIDFSITI
Sbjct: 241  ELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSLKSSCIDFSITI 300

Query: 301  LYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHL 360
            LYV+F+S FLGWALFHPTRER RFS+ EEPLLNIGDDGE+NSVNLEEN+NG TKEH  HL
Sbjct: 301  LYVIFVSAFLGWALFHPTRERSRFSAREEPLLNIGDDGEVNSVNLEENENGATKEHGVHL 360

Query: 361  TLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420
            TL+N VQLSTIQ YI++FYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL
Sbjct: 361  TLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420

Query: 421  WVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNKV 480
            WVGHGS+AA EKQFFD HLAPFYRIEQLIIATKP GR   APRIVTEDNILLLFDIQNKV
Sbjct: 421  WVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDNILLLFDIQNKV 480

Query: 481  NELVANYSGSVVSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYTS 540
            NELVANYSGSVVSL DICLKPL +DCATQSILQY+KMDPENFD+YGGV+H EYC QHYTS
Sbjct: 481  NELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVEHVEYCLQHYTS 540

Query: 541  TETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFI 600
            TETCFSAFKAPLDPSTSLGGFVGNNY+EASAFVVTYPVNNAID VG+EN KAIAWEKAF+
Sbjct: 541  TETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDENGKAIAWEKAFV 600

Query: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSN 660
            KL KEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTI VSYLVMFAYISVALGDSN
Sbjct: 601  KLVKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMFAYISVALGDSN 660

Query: 661  ISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMC 720
            ISSSFY+SSKVLLGLSGV+LVVLSVLGS+GFFSA+GVKSTLIIMEVIPFLVLAVGVDNMC
Sbjct: 661  ISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPFLVLAVGVDNMC 720

Query: 721  ILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780
            ILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA
Sbjct: 721  ILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780

Query: 781  LAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSRY 840
            LAV LDFILQLSAFVALIVFDILRAENHRVDCFPCIKVP  SDE NQGFNQ R GLLSRY
Sbjct: 781  LAVFLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPPRSDEPNQGFNQRRLGLLSRY 840

Query: 841  MKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLA 900
            MKDVHAPLLGLWGVKIAV++IFVGLTLGSI LSTKIEVGLEQKIVLPRDSYLQ YFDDLA
Sbjct: 841  MKDVHAPLLGLWGVKIAVVIIFVGLTLGSITLSTKIEVGLEQKIVLPRDSYLQGYFDDLA 900

Query: 901  EHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPAA 960
            E+LRIGPPLYFVVKDYNYSSKSR+TNQLCSISQCDSNSLLNEISRASLTPEL+YIAKPAA
Sbjct: 901  EYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLTPELNYIAKPAA 960

Query: 961  SWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRHS 1020
            SWLDDFLVWLSPEAFGCCRKFTN SYCPPDDQPPCC PDEG+CD+S GVC+DCTTCF HS
Sbjct: 961  SWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGVCQDCTTCFHHS 1020

Query: 1021 DLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080
            DLVAGRPTTVQF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT
Sbjct: 1021 DLVAGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080

Query: 1081 PLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140
            PLN QGDYVNALRAA+EF +KISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA
Sbjct: 1081 PLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140

Query: 1141 IFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200
            IFIVS VITS LWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV
Sbjct: 1141 IFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200

Query: 1201 HLVHAFSVSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYL 1260
            HLVHAFSVSCGDRSQR++EAL+TMGASVFSGITLTKLVGVIVLCFAKSEIFV+YYFQMYL
Sbjct: 1201 HLVHAFSVSCGDRSQRSQEALTTMGASVFSGITLTKLVGVIVLCFAKSEIFVIYYFQMYL 1260

Query: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRSLAIGDVHEETELLVS 1303
            ALVIIGFLHGLVFLPVILSMIGPPSR     D   ETEL VS
Sbjct: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRYSVQDDAPIETELHVS 1301

BLAST of Sed0023022 vs. ExPASy TrEMBL
Match: A0A1S3BJR3 (Niemann-Pick C1 protein-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490460 PE=3 SV=1)

HSP 1 Score: 2284.6 bits (5919), Expect = 0.0e+00
Identity = 1169/1303 (89.72%), Postives = 1224/1303 (93.94%), Query Frame = 0

Query: 1    MFLNR-GVMASRLRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMY 60
            MFL R G MA RLRFP+SI L QM+F V+ML+GGEA LSV V S ST  E+H+AEYCAMY
Sbjct: 1    MFLTRGGWMAFRLRFPISIFLLQMIFLVSMLMGGEA-LSVPVRSGSTFGERHAAEYCAMY 60

Query: 61   DICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAI 120
            DICG RSDGKVLNCPYGSPSVKPDELFS KIQSLCPTISGNVCCTEAQFETLRSQVQQAI
Sbjct: 61   DICGTRSDGKVLNCPYGSPSVKPDELFSAKIQSLCPTISGNVCCTEAQFETLRSQVQQAI 120

Query: 121  PLFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLY 180
            PLFVGCPAC+RNFLNLFCELSCSPRQSLFINV+SIAEVGG MTVDGIDYYVTEKFGKGLY
Sbjct: 121  PLFVGCPACMRNFLNLFCELSCSPRQSLFINVTSIAEVGGSMTVDGIDYYVTEKFGKGLY 180

Query: 181  DSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQ 240
            DSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKV  PG PGSPY+INFKVN  KSSQ
Sbjct: 181  DSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVA-PGFPGSPYSINFKVNPSKSSQ 240

Query: 241  MELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSIT 300
            MELMNVS YSCGDTSLGCSCGDCP SPVCSSLEPP PPK NACTI+IWS K SCIDFSIT
Sbjct: 241  MELMNVSVYSCGDTSLGCSCGDCPSSPVCSSLEPPSPPKSNACTIKIWSLKISCIDFSIT 300

Query: 301  ILYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAH 360
            ILYV+FIS FLGWALFHPT+E R FSS EEPLLNIGDDGEI SVNL EN+N  T+EH  H
Sbjct: 301  ILYVIFISSFLGWALFHPTKENRGFSSREEPLLNIGDDGEIKSVNLTENENVTTEEHGVH 360

Query: 361  LTLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEK 420
            LT++N VQLSTIQRYISNFYR+YGAWVARNPILVLC SLSIVLILCVGLV FKVETRPEK
Sbjct: 361  LTVRNGVQLSTIQRYISNFYRDYGAWVARNPILVLCMSLSIVLILCVGLVCFKVETRPEK 420

Query: 421  LWVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNK 480
            LWVGHGSRAA EKQFFD +LAPFYRIEQLIIATKP G+H RAPRIVTEDNILLLFDIQNK
Sbjct: 421  LWVGHGSRAAAEKQFFDSNLAPFYRIEQLIIATKPGGKHDRAPRIVTEDNILLLFDIQNK 480

Query: 481  VNELVANYSGSVVSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYT 540
            VNELVANYSGSVVSLNDICLKPL +DCATQSILQY+KM+PENFDDYGGV+HAEYCFQHYT
Sbjct: 481  VNELVANYSGSVVSLNDICLKPLGEDCATQSILQYFKMNPENFDDYGGVEHAEYCFQHYT 540

Query: 541  STETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAF 600
            S+ETCFSAFKAPLDPSTSLGGF G+NY+EASAFV+TYPVNNAIDAVGNEN KAIAWEKAF
Sbjct: 541  SSETCFSAFKAPLDPSTSLGGFFGSNYSEASAFVITYPVNNAIDAVGNENGKAIAWEKAF 600

Query: 601  IKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDS 660
            +KLAKEEL+PLV S+NLTLSFSSESSIEEELKRESTAD+LTI VSYLVMFAYISVALGDS
Sbjct: 601  VKLAKEELMPLVHSRNLTLSFSSESSIEEELKRESTADILTIAVSYLVMFAYISVALGDS 660

Query: 661  NISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNM 720
            NISSSFY+SSKVLLGLSGVILVVLSVLGS+GFFSAIG+KSTLIIMEVIPFLVLAVGVDNM
Sbjct: 661  NISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGIKSTLIIMEVIPFLVLAVGVDNM 720

Query: 721  CILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFA 780
            CILVHAVKRQP+EL+LE+RISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFS+FA
Sbjct: 721  CILVHAVKRQPYELSLEDRISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSLFA 780

Query: 781  ALAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSR 840
            ALAVLLDFILQLSAFVALIV DILRAE+HRVDCFPCIKV  HSDE NQGFNQGR GLLS 
Sbjct: 781  ALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCIKVHPHSDEPNQGFNQGRHGLLSL 840

Query: 841  YMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDL 900
            YMKDVHAPLLG WGVKI V+VIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDL
Sbjct: 841  YMKDVHAPLLGFWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDL 900

Query: 901  AEHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPA 960
            AE+LRIGPPLYFVVKDYNYSS+SR TNQLCSIS CDSNSLLNEISRASLTPEL+YIAKPA
Sbjct: 901  AEYLRIGPPLYFVVKDYNYSSRSRQTNQLCSISHCDSNSLLNEISRASLTPELNYIAKPA 960

Query: 961  ASWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRH 1020
            ASWLDDFLVWLSPEAFGCCRKFTN SYCPPDDQPPCCFPDEG+CDSS GVC+DCTTCFRH
Sbjct: 961  ASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCFPDEGFCDSSEGVCRDCTTCFRH 1020

Query: 1021 SDLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYH 1080
            SDLV  RPTT QF+EKLPWFLNSLPSADCAKGGHGAYTNSVNL GYE GIIKASEFRSYH
Sbjct: 1021 SDLVGDRPTTEQFKEKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYEIGIIKASEFRSYH 1080

Query: 1081 TPLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALG 1140
            TPLN QGDYVNALRAA+EF +KISDSLKMDIFPYSVFYIFFEQYLDIW TALMNIAIALG
Sbjct: 1081 TPLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWNTALMNIAIALG 1140

Query: 1141 AIFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFC 1200
            AIFIVS VITSSLW SG+IILVLAMI+IDL+GVMAIL IQLNAVSVVNILMSIGIAVEFC
Sbjct: 1141 AIFIVSLVITSSLWCSGMIILVLAMIVIDLLGVMAILKIQLNAVSVVNILMSIGIAVEFC 1200

Query: 1201 VHLVHAFSVSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMY 1260
            VHLVHAFSVSCGDR+QRA+EALST+GASVFSGITLTKLVGV+VLCFAKSEIFVVYYFQMY
Sbjct: 1201 VHLVHAFSVSCGDRNQRAQEALSTIGASVFSGITLTKLVGVVVLCFAKSEIFVVYYFQMY 1260

Query: 1261 LALVIIGFLHGLVFLPVILSMIGPPSRSLAIGDVHEETELLVS 1303
            LALVIIGFLHGLVFLPVILSMIGPPSR L   D   ETELLVS
Sbjct: 1261 LALVIIGFLHGLVFLPVILSMIGPPSRYLIKDDAPVETELLVS 1301

BLAST of Sed0023022 vs. ExPASy TrEMBL
Match: A0A1S3BJ43 (Niemann-Pick C1 protein-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490460 PE=3 SV=1)

HSP 1 Score: 2276.5 bits (5898), Expect = 0.0e+00
Identity = 1169/1313 (89.03%), Postives = 1224/1313 (93.22%), Query Frame = 0

Query: 1    MFLNR-GVMASRLRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMY 60
            MFL R G MA RLRFP+SI L QM+F V+ML+GGEA LSV V S ST  E+H+AEYCAMY
Sbjct: 1    MFLTRGGWMAFRLRFPISIFLLQMIFLVSMLMGGEA-LSVPVRSGSTFGERHAAEYCAMY 60

Query: 61   DICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAI 120
            DICG RSDGKVLNCPYGSPSVKPDELFS KIQSLCPTISGNVCCTEAQFETLRSQVQQAI
Sbjct: 61   DICGTRSDGKVLNCPYGSPSVKPDELFSAKIQSLCPTISGNVCCTEAQFETLRSQVQQAI 120

Query: 121  PLFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLY 180
            PLFVGCPAC+RNFLNLFCELSCSPRQSLFINV+SIAEVGG MTVDGIDYYVTEKFGKGLY
Sbjct: 121  PLFVGCPACMRNFLNLFCELSCSPRQSLFINVTSIAEVGGSMTVDGIDYYVTEKFGKGLY 180

Query: 181  DSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQ 240
            DSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKV  PG PGSPY+INFKVN  KSSQ
Sbjct: 181  DSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVA-PGFPGSPYSINFKVNPSKSSQ 240

Query: 241  MELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSIT 300
            MELMNVS YSCGDTSLGCSCGDCP SPVCSSLEPP PPK NACTI+IWS K SCIDFSIT
Sbjct: 241  MELMNVSVYSCGDTSLGCSCGDCPSSPVCSSLEPPSPPKSNACTIKIWSLKISCIDFSIT 300

Query: 301  ILYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAH 360
            ILYV+FIS FLGWALFHPT+E R FSS EEPLLNIGDDGEI SVNL EN+N  T+EH  H
Sbjct: 301  ILYVIFISSFLGWALFHPTKENRGFSSREEPLLNIGDDGEIKSVNLTENENVTTEEHGVH 360

Query: 361  LTLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEK 420
            LT++N VQLSTIQRYISNFYR+YGAWVARNPILVLC SLSIVLILCVGLV FKVETRPEK
Sbjct: 361  LTVRNGVQLSTIQRYISNFYRDYGAWVARNPILVLCMSLSIVLILCVGLVCFKVETRPEK 420

Query: 421  LWVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNK 480
            LWVGHGSRAA EKQFFD +LAPFYRIEQLIIATKP G+H RAPRIVTEDNILLLFDIQNK
Sbjct: 421  LWVGHGSRAAAEKQFFDSNLAPFYRIEQLIIATKPGGKHDRAPRIVTEDNILLLFDIQNK 480

Query: 481  VNELVANYSGSVVSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYT 540
            VNELVANYSGSVVSLNDICLKPL +DCATQSILQY+KM+PENFDDYGGV+HAEYCFQHYT
Sbjct: 481  VNELVANYSGSVVSLNDICLKPLGEDCATQSILQYFKMNPENFDDYGGVEHAEYCFQHYT 540

Query: 541  STETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAF 600
            S+ETCFSAFKAPLDPSTSLGGF G+NY+EASAFV+TYPVNNAIDAVGNEN KAIAWEKAF
Sbjct: 541  SSETCFSAFKAPLDPSTSLGGFFGSNYSEASAFVITYPVNNAIDAVGNENGKAIAWEKAF 600

Query: 601  IKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDS 660
            +KLAKEEL+PLV S+NLTLSFSSESSIEEELKRESTAD+LTI VSYLVMFAYISVALGDS
Sbjct: 601  VKLAKEELMPLVHSRNLTLSFSSESSIEEELKRESTADILTIAVSYLVMFAYISVALGDS 660

Query: 661  NISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNM 720
            NISSSFY+SSKVLLGLSGVILVVLSVLGS+GFFSAIG+KSTLIIMEVIPFLVLAVGVDNM
Sbjct: 661  NISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGIKSTLIIMEVIPFLVLAVGVDNM 720

Query: 721  CILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFA 780
            CILVHAVKRQP+EL+LE+RISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFS+FA
Sbjct: 721  CILVHAVKRQPYELSLEDRISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSLFA 780

Query: 781  ALAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSR 840
            ALAVLLDFILQLSAFVALIV DILRAE+HRVDCFPCIKV  HSDE NQGFNQGR GLLS 
Sbjct: 781  ALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCIKVHPHSDEPNQGFNQGRHGLLSL 840

Query: 841  YMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDL 900
            YMKDVHAPLLG WGVKI V+VIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDL
Sbjct: 841  YMKDVHAPLLGFWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDL 900

Query: 901  AEHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPA 960
            AE+LRIGPPLYFVVKDYNYSS+SR TNQLCSIS CDSNSLLNEISRASLTPEL+YIAKPA
Sbjct: 901  AEYLRIGPPLYFVVKDYNYSSRSRQTNQLCSISHCDSNSLLNEISRASLTPELNYIAKPA 960

Query: 961  ASWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRH 1020
            ASWLDDFLVWLSPEAFGCCRKFTN SYCPPDDQPPCCFPDEG+CDSS GVC+DCTTCFRH
Sbjct: 961  ASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCFPDEGFCDSSEGVCRDCTTCFRH 1020

Query: 1021 SDLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYH 1080
            SDLV  RPTT QF+EKLPWFLNSLPSADCAKGGHGAYTNSVNL GYE GIIKASEFRSYH
Sbjct: 1021 SDLVGDRPTTEQFKEKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYEIGIIKASEFRSYH 1080

Query: 1081 TPLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALG 1140
            TPLN QGDYVNALRAA+EF +KISDSLKMDIFPYSVFYIFFEQYLDIW TALMNIAIALG
Sbjct: 1081 TPLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWNTALMNIAIALG 1140

Query: 1141 AIFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFC 1200
            AIFIVS VITSSLW SG+IILVLAMI+IDL+GVMAIL IQLNAVSVVNILMSIGIAVEFC
Sbjct: 1141 AIFIVSLVITSSLWCSGMIILVLAMIVIDLLGVMAILKIQLNAVSVVNILMSIGIAVEFC 1200

Query: 1201 VHLVHAFS----------VSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSE 1260
            VHLVHAFS          VSCGDR+QRA+EALST+GASVFSGITLTKLVGV+VLCFAKSE
Sbjct: 1201 VHLVHAFSVSTNPTHFITVSCGDRNQRAQEALSTIGASVFSGITLTKLVGVVVLCFAKSE 1260

Query: 1261 IFVVYYFQMYLALVIIGFLHGLVFLPVILSMIGPPSRSLAIGDVHEETELLVS 1303
            IFVVYYFQMYLALVIIGFLHGLVFLPVILSMIGPPSR L   D   ETELLVS
Sbjct: 1261 IFVVYYFQMYLALVIIGFLHGLVFLPVILSMIGPPSRYLIKDDAPVETELLVS 1311

BLAST of Sed0023022 vs. ExPASy TrEMBL
Match: A0A6J1CI77 (Niemann-Pick C1 protein OS=Momordica charantia OX=3673 GN=LOC111011166 PE=3 SV=1)

HSP 1 Score: 2270.7 bits (5883), Expect = 0.0e+00
Identity = 1160/1302 (89.09%), Postives = 1218/1302 (93.55%), Query Frame = 0

Query: 1    MFLNRGVMASRLRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMYD 60
            MFL R  MA  L  P++I L QM+FFV+MLIGGE  LS  V +  TS E+HS EYCAMYD
Sbjct: 1    MFLTREGMAFPLGVPIAIFLLQMIFFVSMLIGGETYLSDPVRNVFTSGERHSTEYCAMYD 60

Query: 61   ICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIP 120
            ICGAR DGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF+TLRSQVQQAIP
Sbjct: 61   ICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFDTLRSQVQQAIP 120

Query: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLYD 180
            LFVGCPACLRNFLNLFCELSCSPRQSLFINV+SI+EVGG MTVDGIDYYVTEKFGKGLYD
Sbjct: 121  LFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDYYVTEKFGKGLYD 180

Query: 181  SCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQM 240
            SCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLG+K   PG PGSPY INFK+N  KSS M
Sbjct: 181  SCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAA-PGFPGSPYTINFKLNTPKSSPM 240

Query: 241  ELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITI 300
            ELMNVS YSCGDTSLGCSCGDCPLSPVCSSLEPP PPK NAC+IRIW  +SSCIDFSITI
Sbjct: 241  ELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFLESSCIDFSITI 300

Query: 301  LYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHL 360
            +Y++FIS FLGWALFHP RE RRFS+ EEPLLNIGD  EI+SVNLEEN+   TKEH A L
Sbjct: 301  IYIIFISAFLGWALFHPARE-RRFSAREEPLLNIGDGHEISSVNLEENEYSATKEHGAQL 360

Query: 361  TLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKL 420
            TL+N +QLSTIQ YISNFYRNYGAWVARNPILVL +SLSIVLILCVGLVRFKVET+PEKL
Sbjct: 361  TLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLVRFKVETQPEKL 420

Query: 421  WVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNKV 480
            WVGHGS+AA EKQFFD  LAPFYRIEQ+IIATKPE RHGRAPRIVTEDNILLLFDIQ+KV
Sbjct: 421  WVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDNILLLFDIQDKV 480

Query: 481  NELVANYSGSVVSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYTS 540
            NELVANYSGS+VSLNDICLKPL +DCATQSILQY+KMDPEN+DDYGGV HAEYCFQHY S
Sbjct: 481  NELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQHAEYCFQHYAS 540

Query: 541  TETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFI 600
            TETCFSAFKAPLDPSTSLGGF GNNY+EASAFVVTYPVNNAIDAVGNEN KA+AWEKAF+
Sbjct: 541  TETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENGKAVAWEKAFV 600

Query: 601  KLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSN 660
            KL KEELLPLVQS+NLTLSFSSESSIEEELKRESTADVLTIVVSYLVMF YISVALGDS+
Sbjct: 601  KLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFVYISVALGDSS 660

Query: 661  ISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMC 720
            ISSSFY+SSKVLLGLSGVILVVLSVLGS+GFFSAIGVKSTLIIMEVIPFLVLAVGVDNMC
Sbjct: 661  ISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMC 720

Query: 721  ILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAA 780
            ILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVG FVPMPACRVFSMFAA
Sbjct: 721  ILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMPACRVFSMFAA 780

Query: 781  LAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSRY 840
            LAVLLDFILQLSAFVALIVFDILRAE+HRVDCFPCIKV  HSDE NQGFNQG+PGLLS Y
Sbjct: 781  LAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFNQGKPGLLSWY 840

Query: 841  MKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLA 900
            MKDVHAPLLGLWGVKIAVIVIFVG TLG IALSTKIEVGLEQKIVLPRDSYLQDYFDDLA
Sbjct: 841  MKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDSYLQDYFDDLA 900

Query: 901  EHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPAA 960
            E+LRIGPPLYFVVKDYNYSS+SR TNQLCSISQCDSNSLLNEISRASLTPEL+YIAKPAA
Sbjct: 901  EYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLTPELNYIAKPAA 960

Query: 961  SWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRHS 1020
            SWLDDFLVWLSP+AFGCCRKFTN SYCPPDDQPPCCFPDEG CDSS GVCKDCTTCF +S
Sbjct: 961  SWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGVCKDCTTCFHYS 1020

Query: 1021 DLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHT 1080
            DLVAGRPTT+QFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFR+YHT
Sbjct: 1021 DLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRTYHT 1080

Query: 1081 PLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGA 1140
            PLN QGDYVNALRAA+EFS+K+SDSLKMDIFPYSVFYIFFEQYLDIWK AL+NIA+ALGA
Sbjct: 1081 PLNKQGDYVNALRAAKEFSSKMSDSLKMDIFPYSVFYIFFEQYLDIWKIALINIAVALGA 1140

Query: 1141 IFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCV 1200
            +F+VS VITS LWSSGIIILVLAMIIIDLMG+MAILNIQLNAVSVVN+LMSIGIAVEFCV
Sbjct: 1141 VFVVSLVITSCLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLMSIGIAVEFCV 1200

Query: 1201 HLVHAFSVSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYL 1260
            H+VHAF VS GDRSQRAREAL TMGASVFSGITLTKLVGVIVLCF++SEIFVVYYFQMYL
Sbjct: 1201 HIVHAFLVSSGDRSQRAREALGTMGASVFSGITLTKLVGVIVLCFSRSEIFVVYYFQMYL 1260

Query: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRSLAIGDVHEETELLVS 1303
            ALVIIGFLHGLVFLPVILSMIGPPSR L   D   ETELLVS
Sbjct: 1261 ALVIIGFLHGLVFLPVILSMIGPPSRYLVKDDAPTETELLVS 1300

BLAST of Sed0023022 vs. TAIR 10
Match: AT4G38350.1 (Patched family protein )

HSP 1 Score: 1821.6 bits (4717), Expect = 0.0e+00
Identity = 928/1279 (72.56%), Postives = 1075/1279 (84.05%), Query Frame = 0

Query: 12   LRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMYDICGARSDGKVL 71
            +  P  + L Q+  F  +L    A+L+   HS+  S  +HS EYCAMYDICG RSDGKVL
Sbjct: 1    MAIPTKLLLLQLFTFSLLL---TANLT---HSE-PSNNRHSKEYCAMYDICGHRSDGKVL 60

Query: 72   NCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIPLFVGCPACLRN 131
            NCPY SPS++PDELFS KIQSLCPTISGNVCCTE QF+TLRSQVQQA+P  VGCPACLRN
Sbjct: 61   NCPYASPSIQPDELFSAKIQSLCPTISGNVCCTETQFDTLRSQVQQAVPFLVGCPACLRN 120

Query: 132  FLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLYDSCKDVKFGTMN 191
            FLNLFCELSCSP QSLFINV+S+AEV G +TVDGIDY++T+ FG+GLY+SCK+VKFGTMN
Sbjct: 121  FLNLFCELSCSPNQSLFINVTSVAEVSGNLTVDGIDYHITDTFGEGLYESCKEVKFGTMN 180

Query: 192  TRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQMELMNVSAYSCG 251
            TRAI+FVGGGAK+F E F F+GQK    G PGSPYAINFK +  +SS M  MNVS YSCG
Sbjct: 181  TRAINFVGGGAKNFREWFTFIGQK-APSGFPGSPYAINFKSSIPESSAMVPMNVSVYSCG 240

Query: 252  DTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITILYVLFISVFLG 311
            DTSLGCSCGDCP SP CSS EP PP   ++C+IRI   K  CI+ S+ ++YVL +S F G
Sbjct: 241  DTSLGCSCGDCPSSPACSSPEPLPPHDEDSCSIRIGPLKVRCIELSMALVYVLLVSCFFG 300

Query: 312  WALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHLTLKNDVQLSTI 371
            WA  +  R   +     +PLL+  ++  INS           KE++  + ++   QLS +
Sbjct: 301  WAGLNRRRNTTQPLDSSKPLLHPVEEDGINS---------EMKENILGVKVQRHAQLSPV 360

Query: 372  QRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKLWVGHGSRAAVE 431
            QRY++ FYR+YG+W+ARNP LVL  S++IVL LC GL  FKVETRPEKLWVG  S+AA E
Sbjct: 361  QRYMAKFYRSYGSWIARNPSLVLFMSVAIVLALCSGLYNFKVETRPEKLWVGPESKAAEE 420

Query: 432  KQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNKVNELVANYSGSV 491
            K+FFD HL+PFYRIEQLI+AT P+ + GRAP IVT++NILLLFDIQ KV+++  NYSGS 
Sbjct: 421  KKFFDTHLSPFYRIEQLILATVPDPKSGRAPSIVTDENILLLFDIQQKVDQIRGNYSGSE 480

Query: 492  VSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYTSTETCFSAFKAP 551
            VSL DICLKPL +DCATQSILQY+KMD   FDDYGGV+HAEYCFQHYTS+ETC SAF+AP
Sbjct: 481  VSLPDICLKPLGEDCATQSILQYFKMDSGTFDDYGGVEHAEYCFQHYTSSETCLSAFQAP 540

Query: 552  LDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAVGNENEKAIAWEKAFIKLAKEELLPLV 611
            +DPS  LGGF GNNY+EA+AFVVTYPVNN I    NEN +A+AWEK+FI+LAKEELLP+V
Sbjct: 541  VDPSAVLGGFSGNNYSEATAFVVTYPVNNVIGDSSNENARAVAWEKSFIQLAKEELLPMV 600

Query: 612  QSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFAYISVALGDSNISSSFYISSKV 671
            +SKNL+LSFSSESSIEEELKRESTADV+TI  SYLVMF YISV LGD+    +FYISSKV
Sbjct: 601  RSKNLSLSFSSESSIEEELKRESTADVITIAASYLVMFVYISVTLGDAPQFYTFYISSKV 660

Query: 672  LLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPF 731
            LLGLSGV+LV+LSVLGS+G FSA+GVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQP 
Sbjct: 661  LLGLSGVVLVLLSVLGSVGVFSALGVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPR 720

Query: 732  ELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAALAVLLDFILQL 791
            E++LE+RISSALVEVGPSITLASLSE+LAFAVG FVPMPACR+FSMFAALA++LDF LQ+
Sbjct: 721  EVSLEQRISSALVEVGPSITLASLSEVLAFAVGAFVPMPACRIFSMFAALAIMLDFFLQI 780

Query: 792  SAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQGFNQGRPGLLSRYMKDVHAPLLGL 851
            +AFVALIVFD  R+ ++R+DCFPCIKVPS S E  +G  +  PG L RYMK+VHAP+LGL
Sbjct: 781  TAFVALIVFDCKRSADNRIDCFPCIKVPSSSRESVEGGRE--PGFLERYMKEVHAPVLGL 840

Query: 852  WGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLAEHLRIGPPLYF 911
            WGVK+ V+ +F    L SIA+S ++E GLEQKIVLPRDSYLQDYFD L+E+LR+GPPLYF
Sbjct: 841  WGVKMVVVAVFFAFALASIAISPRLETGLEQKIVLPRDSYLQDYFDSLSEYLRVGPPLYF 900

Query: 912  VVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRASLTPELSYIAKPAASWLDDFLVWLS 971
            VVK+YNYSS+SR+TNQLCSISQC+SNSLLNEISRAS   + SYIAKPAASWLDDFLVWLS
Sbjct: 901  VVKNYNYSSESRHTNQLCSISQCNSNSLLNEISRASQASDTSYIAKPAASWLDDFLVWLS 960

Query: 972  PEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSAGVCKDCTTCFRHSDLVAGRPTTVQ 1031
            PEAFGCCRKFTN SYCPPDDQPPCC  +E  C S  G+CKDCTTCFRHSDLV  RP+T Q
Sbjct: 961  PEAFGCCRKFTNGSYCPPDDQPPCCTAEEDIC-SLDGICKDCTTCFRHSDLVQDRPSTAQ 1020

Query: 1032 FQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNNQGDYVNA 1091
            F+EKLPWFLN+LPSADCAKGGHGAYTNSV+L GYESG+I+ASEFR+YHTPLN QGDYVNA
Sbjct: 1021 FREKLPWFLNALPSADCAKGGHGAYTNSVDLKGYESGVIQASEFRTYHTPLNTQGDYVNA 1080

Query: 1092 LRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSFVITSS 1151
            LRAA+EFS++IS+SLK+DIFPYSVFYIFFEQYL+IW  AL N+AIA+GAIFIV ++ITSS
Sbjct: 1081 LRAAREFSSRISNSLKIDIFPYSVFYIFFEQYLNIWTVALTNLAIAIGAIFIVCWLITSS 1140

Query: 1152 LWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCG 1211
             WSS II+LVL MI++DLMG+M IL IQLNAVSVVN++MSIGIAVEFCVH+ HAF +S G
Sbjct: 1141 AWSSAIIVLVLVMILVDLMGMMVILGIQLNAVSVVNLIMSIGIAVEFCVHISHAFLMSSG 1200

Query: 1212 DRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYLALVIIGFLHGL 1271
            DR  RAREAL TMGASVFSGITLTKLVGVIVLCFA+SEIFVVYYFQMYLALVIIGFLHGL
Sbjct: 1201 DREHRAREALETMGASVFSGITLTKLVGVIVLCFARSEIFVVYYFQMYLALVIIGFLHGL 1259

Query: 1272 VFLPVILSMIGPPSRSLAI 1291
            VFLPVILS+ GPP  +L I
Sbjct: 1261 VFLPVILSLAGPPQLNLDI 1259

BLAST of Sed0023022 vs. TAIR 10
Match: AT4G38350.2 (Patched family protein )

HSP 1 Score: 1808.1 bits (4682), Expect = 0.0e+00
Identity = 928/1303 (71.22%), Postives = 1075/1303 (82.50%), Query Frame = 0

Query: 12   LRFPVSISLFQMLFFVTMLIGGEADLSVSVHSDSTSEEKHSAEYCAMYDICGARSDGKVL 71
            +  P  + L Q+  F  +L    A+L+   HS+  S  +HS EYCAMYDICG RSDGKVL
Sbjct: 1    MAIPTKLLLLQLFTFSLLL---TANLT---HSE-PSNNRHSKEYCAMYDICGHRSDGKVL 60

Query: 72   NCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFETLRSQVQQAIPLFVGCPACLRN 131
            NCPY SPS++PDELFS KIQSLCPTISGNVCCTE QF+TLRSQVQQA+P  VGCPACLRN
Sbjct: 61   NCPYASPSIQPDELFSAKIQSLCPTISGNVCCTETQFDTLRSQVQQAVPFLVGCPACLRN 120

Query: 132  FLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGIDYYVTEKFGKGLYDSCKDVKFGTMN 191
            FLNLFCELSCSP QSLFINV+S+AEV G +TVDGIDY++T+ FG+GLY+SCK+VKFGTMN
Sbjct: 121  FLNLFCELSCSPNQSLFINVTSVAEVSGNLTVDGIDYHITDTFGEGLYESCKEVKFGTMN 180

Query: 192  TRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYAINFKVNADKSSQMELMNVSAYSCG 251
            TRAI+FVGGGAK+F E F F+GQK    G PGSPYAINFK +  +SS M  MNVS YSCG
Sbjct: 181  TRAINFVGGGAKNFREWFTFIGQK-APSGFPGSPYAINFKSSIPESSAMVPMNVSVYSCG 240

Query: 252  DTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIWSFKSSCIDFSITILYVLFISVFLG 311
            DTSLGCSCGDCP SP CSS EP PP   ++C+IRI   K  CI+ S+ ++YVL +S F G
Sbjct: 241  DTSLGCSCGDCPSSPACSSPEPLPPHDEDSCSIRIGPLKVRCIELSMALVYVLLVSCFFG 300

Query: 312  WALFHPTRERRRFSSGEEPLLNIGDDGEINSVNLEENDNGPTKEHVAHLTLKNDVQLSTI 371
            WA  +  R   +     +PLL+  ++  INS           KE++  + ++   QLS +
Sbjct: 301  WAGLNRRRNTTQPLDSSKPLLHPVEEDGINS---------EMKENILGVKVQRHAQLSPV 360

Query: 372  QRYISNFYRNYGAWVARNPILVLCTSLSIVLILCVGLVRFKVETRPEKLWVGHGSRAAVE 431
            QRY++ FYR+YG+W+ARNP LVL  S++IVL LC GL  FKVETRPEKLWVG  S+AA E
Sbjct: 361  QRYMAKFYRSYGSWIARNPSLVLFMSVAIVLALCSGLYNFKVETRPEKLWVGPESKAAEE 420

Query: 432  KQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVTEDNILLLFDIQNKVNELVANYSGSV 491
            K+FFD HL+PFYRIEQLI+AT P+ + GRAP IVT++NILLLFDIQ KV+++  NYSGS 
Sbjct: 421  KKFFDTHLSPFYRIEQLILATVPDPKSGRAPSIVTDENILLLFDIQQKVDQIRGNYSGSE 480

Query: 492  VSLNDICLKPLDKDCATQSILQYYKMDPENFDDYGGVDHAEYCFQHYTSTETCFSAFKAP 551
            VSL DICLKPL +DCATQSILQY+KMD   FDDYGGV+HAEYCFQHYTS+ETC SAF+AP
Sbjct: 481  VSLPDICLKPLGEDCATQSILQYFKMDSGTFDDYGGVEHAEYCFQHYTSSETCLSAFQAP 540

Query: 552  LDPSTSLGGFVGNNYTE------------------------ASAFVVTYPVNNAIDAVGN 611
            +DPS  LGGF GNNY+E                        A+AFVVTYPVNN I    N
Sbjct: 541  VDPSAVLGGFSGNNYSEVMVSELGCSVPFDCYSDVKRTLFQATAFVVTYPVNNVIGDSSN 600

Query: 612  ENEKAIAWEKAFIKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSYLV 671
            EN +A+AWEK+FI+LAKEELLP+V+SKNL+LSFSSESSIEEELKRESTADV+TI  SYLV
Sbjct: 601  ENARAVAWEKSFIQLAKEELLPMVRSKNLSLSFSSESSIEEELKRESTADVITIAASYLV 660

Query: 672  MFAYISVALGDSNISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIMEVI 731
            MF YISV LGD+    +FYISSKVLLGLSGV+LV+LSVLGS+G FSA+GVKSTLIIMEVI
Sbjct: 661  MFVYISVTLGDAPQFYTFYISSKVLLGLSGVVLVLLSVLGSVGVFSALGVKSTLIIMEVI 720

Query: 732  PFLVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFV 791
            PFLVLAVGVDNMCILVHAVKRQP E++LE+RISSALVEVGPSITLASLSE+LAFAVG FV
Sbjct: 721  PFLVLAVGVDNMCILVHAVKRQPREVSLEQRISSALVEVGPSITLASLSEVLAFAVGAFV 780

Query: 792  PMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDERNQ 851
            PMPACR+FSMFAALA++LDF LQ++AFVALIVFD  R+ ++R+DCFPCIKVPS S E  +
Sbjct: 781  PMPACRIFSMFAALAIMLDFFLQITAFVALIVFDCKRSADNRIDCFPCIKVPSSSRESVE 840

Query: 852  GFNQGRPGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLP 911
            G  +  PG L RYMK+VHAP+LGLWGVK+ V+ +F    L SIA+S ++E GLEQKIVLP
Sbjct: 841  GGRE--PGFLERYMKEVHAPVLGLWGVKMVVVAVFFAFALASIAISPRLETGLEQKIVLP 900

Query: 912  RDSYLQDYFDDLAEHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISRAS 971
            RDSYLQDYFD L+E+LR+GPPLYFVVK+YNYSS+SR+TNQLCSISQC+SNSLLNEISRAS
Sbjct: 901  RDSYLQDYFDSLSEYLRVGPPLYFVVKNYNYSSESRHTNQLCSISQCNSNSLLNEISRAS 960

Query: 972  LTPELSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDSSA 1031
               + SYIAKPAASWLDDFLVWLSPEAFGCCRKFTN SYCPPDDQPPCC  +E  C S  
Sbjct: 961  QASDTSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCTAEEDIC-SLD 1020

Query: 1032 GVCKDCTTCFRHSDLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYES 1091
            G+CKDCTTCFRHSDLV  RP+T QF+EKLPWFLN+LPSADCAKGGHGAYTNSV+L GYES
Sbjct: 1021 GICKDCTTCFRHSDLVQDRPSTAQFREKLPWFLNALPSADCAKGGHGAYTNSVDLKGYES 1080

Query: 1092 GIIKASEFRSYHTPLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLDIW 1151
            G+I+ASEFR+YHTPLN QGDYVNALRAA+EFS++IS+SLK+DIFPYSVFYIFFEQYL+IW
Sbjct: 1081 GVIQASEFRTYHTPLNTQGDYVNALRAAREFSSRISNSLKIDIFPYSVFYIFFEQYLNIW 1140

Query: 1152 KTALMNIAIALGAIFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVN 1211
              AL N+AIA+GAIFIV ++ITSS WSS II+LVL MI++DLMG+M IL IQLNAVSVVN
Sbjct: 1141 TVALTNLAIAIGAIFIVCWLITSSAWSSAIIVLVLVMILVDLMGMMVILGIQLNAVSVVN 1200

Query: 1212 ILMSIGIAVEFCVHLVHAFSVSCGDRSQRAREALSTMGASVFSGITLTKLVGVIVLCFAK 1271
            ++MSIGIAVEFCVH+ HAF +S GDR  RAREAL TMGASVFSGITLTKLVGVIVLCFA+
Sbjct: 1201 LIMSIGIAVEFCVHISHAFLMSSGDREHRAREALETMGASVFSGITLTKLVGVIVLCFAR 1260

Query: 1272 SEIFVVYYFQMYLALVIIGFLHGLVFLPVILSMIGPPSRSLAI 1291
            SEIFVVYYFQMYLALVIIGFLHGLVFLPVILS+ GPP  +L I
Sbjct: 1261 SEIFVVYYFQMYLALVIIGFLHGLVFLPVILSLAGPPQLNLDI 1283

BLAST of Sed0023022 vs. TAIR 10
Match: AT1G42470.1 (Patched family protein )

HSP 1 Score: 1617.4 bits (4187), Expect = 0.0e+00
Identity = 812/1162 (69.88%), Postives = 972/1162 (83.65%), Query Frame = 0

Query: 48   EEKHSAEYCAMYDICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQ 107
            E K SA YCAMYDICGARSDGKVLNCP+  PSVKPD+L S KIQSLCPTI+GNVCCTE Q
Sbjct: 11   EAKQSAGYCAMYDICGARSDGKVLNCPFNIPSVKPDDLLSSKIQSLCPTITGNVCCTETQ 70

Query: 108  FETLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVSSIAEVGGKMTVDGID 167
            F+TLRSQVQQAIP  VGCPACLRNFLNLFCEL+CSP QSLFINV+S  +V    TVDGI 
Sbjct: 71   FDTLRSQVQQAIPFIVGCPACLRNFLNLFCELTCSPDQSLFINVTSTTKVKNNSTVDGIQ 130

Query: 168  YYVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVVDPGLPGSPYA 227
            YY+T+ FG G+Y+SCK+VKFG+ N+RA+DF+G GAK+F+E F F+GQK     LPGSPY 
Sbjct: 131  YYITDDFGAGMYESCKNVKFGSSNSRALDFLGAGAKNFKEWFTFIGQK-AGVNLPGSPYG 190

Query: 228  INFKVNADKSSQMELMNVSAYSCGDTSLGCSCGDCPLSPVCSSLEPPPPPKRNACTIRIW 287
            I F      SS M  MNVS YSCGD SLGCSCGDCP +  CSS    P  K+++C+I+I 
Sbjct: 191  IAFLPTPPVSSGMRPMNVSIYSCGDESLGCSCGDCPSAATCSSKAEVPTQKKHSCSIKIG 250

Query: 288  SFKSSCIDFSITILYVLFISVFLGWALFHPTRERRRFSSGEEPLLNIGD-DGEINSVNLE 347
            S +  C+DF + ILY++ +S+FLG  L HP R +++ S     +  + +  GE NSVN +
Sbjct: 251  SLEVKCVDFILAILYIVLVSLFLGGGLLHPVRGKKKTSQ----MGTLSEASGERNSVNQQ 310

Query: 348  ENDNGPTKEHVAHLTLKNDVQLSTIQRYISNFYRNYGAWVARNPILVLCTSLSIVLILCV 407
            + D     + + +   +N  QLST+Q +++NFY  YG WVAR+P LVLC S+S+VL+LCV
Sbjct: 311  KPDT-IQSQMLQNTPQRNWGQLSTVQGHLANFYGKYGIWVARHPTLVLCLSVSVVLLLCV 370

Query: 408  GLVRFKVETRPEKLWVGHGSRAAVEKQFFDRHLAPFYRIEQLIIATKPEGRHGRAPRIVT 467
            GL+RFKVETRP+KLWVG GSRAA EKQFFD HLAPFYRIEQLIIAT     H +AP I+T
Sbjct: 371  GLIRFKVETRPDKLWVGSGSRAAEEKQFFDTHLAPFYRIEQLIIATVQTSSHEKAPEILT 430

Query: 468  EDNILLLFDIQNKVNELVANYSGSVVSLNDICLKPLDKDCATQSIL-QYYKMDPENFDDY 527
            +DNI LLFDIQ KV+ L AN+SGS+VSL DIC+KPL +DCATQS+L QY+KM PEN+DDY
Sbjct: 431  DDNIKLLFDIQKKVDGLRANHSGSMVSLTDICMKPLGEDCATQSVLQQYFKMKPENYDDY 490

Query: 528  GGVDHAEYCFQHYTSTETCFSAFKAPLDPSTSLGGFVGNNYTEASAFVVTYPVNNAIDAV 587
            GGVDH +YCF+H+TSTE+C SAFK PLDP+T+LGGF GN+++EASAF+VTYPV+N +D  
Sbjct: 491  GGVDHVKYCFEHFTSTESCLSAFKGPLDPTTALGGFSGNSFSEASAFLVTYPVDNFVDNK 550

Query: 588  GNENEKAIAWEKAFIKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIVVSY 647
            GN+ EKA+AWEKAFI+LAK+ELLP+VQ+KNLTLSFSSESSIEEELKRESTADV+TI +SY
Sbjct: 551  GNKTEKAVAWEKAFIQLAKDELLPMVQAKNLTLSFSSESSIEEELKRESTADVITIAISY 610

Query: 648  LVMFAYISVALGDSNISSSFYISSKVLLGLSGVILVVLSVLGSIGFFSAIGVKSTLIIME 707
            LVMFAYIS+ LGDS    SFYI+SKVLLGLSGV+LV+LSVLGS+GFFSA+G+KSTLIIME
Sbjct: 611  LVMFAYISLTLGDSPRLKSFYITSKVLLGLSGVLLVMLSVLGSVGFFSAVGMKSTLIIME 670

Query: 708  VIPFLVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGT 767
            VIPFLVLAVGVDNMCILVHAVKRQ  EL LE RIS+AL+EVGPSITLASL+EILAFAVG 
Sbjct: 671  VIPFLVLAVGVDNMCILVHAVKRQEQELPLERRISNALMEVGPSITLASLAEILAFAVGA 730

Query: 768  FVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPSHSDER 827
            F+ MPA RVFSMFAALAVLLDF+LQ++AFVALIVFD  R E+ RVDCFPCIK    S   
Sbjct: 731  FIKMPAVRVFSMFAALAVLLDFLLQITAFVALIVFDFRRTEDKRVDCFPCIKTSKSSISA 790

Query: 828  NQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIV 887
             +G  Q + GLL+RYMK+VHAP+L  W VKI VI  F GL +  IALST+IE GLEQ+IV
Sbjct: 791  EKGVGQRKAGLLTRYMKEVHAPVLSHWIVKIVVIAFFFGLAMAGIALSTRIEPGLEQQIV 850

Query: 888  LPRDSYLQDYFDDLAEHLRIGPPLYFVVKDYNYSSKSRNTNQLCSISQCDSNSLLNEISR 947
            LP+DSYLQ YF++++ +LRIGPPLYFV+K+YNYSS+SR+TNQLCSI++C+ NSLLNEI+R
Sbjct: 851  LPQDSYLQGYFNNISTYLRIGPPLYFVLKNYNYSSESRHTNQLCSINKCNPNSLLNEIAR 910

Query: 948  ASLTPELSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNSSYCPPDDQPPCCFPDEGYCDS 1007
            ASLTPELSYIAKPAASWLDDFLVWLSPEAFGCCRKFTN ++CPPDDQPPCC P +  C  
Sbjct: 911  ASLTPELSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGTFCPPDDQPPCCPPGQTSCGL 970

Query: 1008 SAGVCKDCTTCFRHSDLVAGRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGY 1067
            S  VCKDCTTCFRH+DL + RP+T QF+EKLPWFLN+LPSADCAKGGHGAY++SV+L GY
Sbjct: 971  SE-VCKDCTTCFRHADLSSDRPSTTQFKEKLPWFLNALPSADCAKGGHGAYSSSVDLQGY 1030

Query: 1068 ESGIIKASEFRSYHTPLNNQGDYVNALRAAQEFSAKISDSLKMDIFPYSVFYIFFEQYLD 1127
             +GII+AS FR+YHTPLN Q D+VN++RAAQEFSAK+S SLKM+I+PYSVFY+FFEQYLD
Sbjct: 1031 ANGIIQASSFRTYHTPLNKQVDFVNSMRAAQEFSAKVSRSLKMEIYPYSVFYMFFEQYLD 1090

Query: 1128 IWKTALMNIAIALGAIFIVSFVITSSLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSV 1187
            IWKTAL+N++IA+ A+F+V  +IT S WSS II+LV+AMIIIDL+GVMA+ +IQLNA+SV
Sbjct: 1091 IWKTALINLSIAIAAVFVVCLIITCSFWSSAIILLVIAMIIIDLLGVMAVFHIQLNALSV 1150

Query: 1188 VNILMSIGIAVEFCVHLVHAFS 1208
            VN++MS+GIAVEFCVH+ HAFS
Sbjct: 1151 VNLIMSVGIAVEFCVHITHAFS 1165