Sed0022840 (gene) Chayote v1

Overview
NameSed0022840
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPectinesterase
LocationLG07: 4232363 .. 4236474 (-)
RNA-Seq ExpressionSed0022840
SyntenySed0022840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATGAAATGGGTCCAAACAAAAGCAACCTCCAAGTTGAATAGATTTTTAAGAATCAATTAACAAATAAATCCCAAAAAGAAAAAAAAAAACCCCACCCTCCTCTCTTCTATAAATACACACAAACCGAAACTCCAAATCCCCAATTCCCAAATCCGAAAAACAGAGCCATTTACAGAGTCCCTTCTTTGTGCAATGGCGGCCACACCATTCATCTACTTTCTCGTCCTCCTCTGCGTCTCCACCGCCGTCTCCGCCGCCGCCGCAACCCAAACCGGGTCGGTCTGCAGGTCGACCCTGGACCCGGCGTTCTGCAAAACCGCCCTCCCGAACCAAACCGGCAATGTGTACGCGTACGGGCGGTCGTCGGTGAGGAAATCGCTGTCGTCGTCGCAGAAGTTTCTGCGGACGGTGGAGAAGCAGCTCCGGGGGAGGGCGTGGCTAACGGTGGGGGCGGTGAGGGCGCTGGAGGATTGCCAGCTATTAGCCGGGTTGAACATCGATTACTTGGCGGCGGCGATGACGACGGTGGACCGGACGAGCTCGGCGTTGCCGGAGATGAAGGCGGAGGAGGTGGCGAGCCTGTTGAGTGCGCTGTTGACGAACCAGCAGACGTGTATGGATGGGCTGAAGGCGACGGCGGGTTCTTGGTCTCTCAAAAATGGACTCTCTCTCCCACTTGCTACTGACACCAAACTCTACTCTATGTCCTTGGCTCTATTTACCAAAGGTTTGTTTTCTTTTTATTTATGTCATTCAAGTTATTATATATAAAATAATGTCTAATTATGTAGTTTTATATTATAAGATATTTTTGTTATCTTTTAAATTTTAAATTAAATAGATTGTAACTCATATTAAGTCGAGTAACTTTTATGGGAGTATTTGCTTAATTTTGTTTATTCCTAGTAGGTGAAGAGTAATGGTGATGTTGATTTGTTTCTTTTAATGCTAATTATTAGAATTTTTTTAAAACCATGTCTAGATATTATTATTGGTGTAGTTTAGTACATTTTTAGTTACTTTTCCAGTTAAAATTTGAATTTATGTCGCTTGCTTATGAATGAATATATGCAATGCCGATTAGTTTTTGCTCTTTATGGCTATATTCATCCATTTATTAGTTAAATGAAATTATAAGAGTTTCAATCGACTTCTTTTGTGTTCTCTTTTCTTTTTTTTTGAAAACATCTTTTGTGTTCTCTTTAATTCTTATTAATATTGTCAATTTATTATTTTGTTAACTTTAAAATATGTTAATAATGAGGCGAAGAAATTTAGTACACATTTCTTTCTCTCTCAAAAAATAATAGAAATTTAGTAAACACGTCATTTTTTTTAATTATAAGTACAAATTTCTAATGTATTAATTGGTAGGTCAGTTTGTTGAAAGTTTTATTTGGTTGTTGCAAGGGTTAGTTCTCTGTTTCTCTCTCTCTTTTTTTTTAGAATAGCTTACAAATCTAATGAAGTTCAAAATATCTTTTTTGTATTTTAGTTCCATAATAATTGAAGTGCAATTAGAACTATAGTAATAAACACAAATACTATTAAATATTTTAATTTCTAAATTATATTTAAAAATAATAAACTTCTTTATATTCCACCTATAAGGAAATTTTTAAAAAAACGCCTAGAAGAATAACAGATATTAATGTATACTCAATAACCTATAGATTTTTTTGTTTGAATTTCCCTCCCCAATCACGGTAAAACTTAGGGAAAAAAATACAAAATACCAATACATTTAAAATTTACCAATTAATTAAATCAACAACAAACATTATTGGTTCCCGACAGGTTGGGTACCGAAGAAAAAGAAAAGGTCGACATGGAAGGGGGCCGGAAAACAGGGCGGGTTCGGAAACGGCCGAATGTCGGTAAAAATGTCGAGCAGAGCCAAAGCGATCTACGAGGCGGCGGCTCGACGGAATCTGCAACAGAGCAACGACGAGGACGGCGGCGACGACCAGATATCGGTTCGGGACATCGTGGTGGTGAGCCAGGATGGGAGCGGGAATTTCACGACGATTAACGAGGCGATCGCGGCGGCGCCGAACAACTCTGCTCCGACGGAGGGGTATTTCTTGATCTTTGTGTCGGCCGGAGTTTATGAAGAGTATGTTTCGATTGCGAAGAACAAGAGGTACTTGATGATGATCGGAGATGGGATTAATCAGACCGTTATTACCGGTAATCGGAGCGTCGTTGACGGCTGGACCACTTTCAATTCCGCCACCTTTGGTAAGCCTTTCCTATTTCAATTATTATGACTTTACGTAATTGGTCAATTTAGTTACTCTACTTTTTGAAAAATATAATAAAATTTAAAAAACTTACATTTTTTTTTTAAATTTTTTTATTTTTGTGTATAATATTGTTATATCACATAATTGGGGGGTTTTTGCACCTCACGTGATTTGTTTTTTTTTAAATAAATCAATTATTTTTCTTGATATCAATCATTTTCATTTTGGATTCTAATGCTTTGAATCTTTCAATTTCAGTCAATTTTTCTTGAAGTTTCTCTATAGCTTAAATTTTAGTTATATGTCTAATGAACTCTTAAAATTTAAAAGCATTTGACATATTTTCATTTTAAATTTAAATTTTTTTTCTCATGACTGTAATGTTGAATTGGATTATCACTAAATAATGAAATTAATATGCCAATAATCCTTTCAATTTTATGATTCAATATTAATTTACTACTATACATATCAATTATTGCCAAGTGAAACTCAATATGTTTTAAATTTTATATTGAATGTCATATCACATATAACAATATAATGAGGCTAATTATGTATTAGAGACTTGAAATTCTTAATAGTTTCAAATGTCGACCATCCAATCTCCAATCATTACGAGTAATATCTTAGTAGTAACATGATTATGAAGAAGAATATAAATTTGAATCCTCAATCTAAATTATTATTGAACTAAAAGAATTCAATTTTCATAAGTATTAATTAAAAATTTTATAGCCGCGAGAGTGCTAGCTTAACAGTATTAATGTATATATGCCTTTGGACCAAGTGATCATGGGTTGAATCCTCCCTTTGTTGTTAAAACAGAACTAAAATTTCTTATACAATTTTTAGAATTGGACTAAATTGTTGTTGTTTTAATTTGATTTTGTTCTTCCCATTTTTTGACCTGTAATTCAACTGTTATTGCCATCATCAGCGGTGGTAGGCCCAGGATTCGTAGCAGTAAACATGACATTCAGAAACACAGCCGGAGCAGAAAAGCACCAAGCCGTGGCGGTCCGGAGCGGCGCCGACTTATCGACGTTCTACCTCTGCAGCTTCGAAGCCTACCAAGACACATTATACACCCACTCCCTCCGCCAGTTCTACCGCGACTGCGACATCTACGGAACCGTCGACTTCATCTTCGGCAATGCAGCCGTCGTCTTCCAAAACTGCAACATCCACCCCCGCCTTCCCATGGCCAACCAGTTCAACGCCATCACCGCCCAAGGCCGCACCGACCCGAATCAGAACACCGGCACCTCGATCCAGAACTGTCGGATCACGCCCGCCGACGACCTCGCCAATGCCACTGCTGTCAAGACCTTCCTCGGCCGGCCGTGGAAGGAGTTCTCCCGGACGGTTTATTTGCAGAATTTTATGGATGGTTTTATTAATCCGGCTGGGTGGAGGGCTTGGGATGGGGAGTTTGCTCTTAATACTTCGTATTATGCTGAGTTTGGGAATTTTGGACCGGGTTCGAACACGTCCGAGCGGGTTAATTGGACCGGGTTTCATTTGATTAATGATACTGATGCTGGGAATTTTACGGCGGCCAGTTTTGTGTTGGCCGATGATTGGCTGCCGCAGACCGGCGTTCCGTACACCAGTGGCTTGACGGAGTAGGGAAACAGGGGAATTTTGATTTTTGGAAAAAAAATTAATTTTAACTTCTTTTTTGTTAATTTAAAAAATGAGAAATTCCAAAGGCTAAATGTATAGCTTGGGATGATATGATGATATGGTTGATTCTTTTTGTTATTCCATTTTTATTTTCTGGTGAAAGAAATAGAATAAATATCTACTTTTTGTTCATGAGATTTTCTTTTTTTCAGAAATATTGAAGTCTTTTGTCTGGA

mRNA sequence

CATGAAATGGGTCCAAACAAAAGCAACCTCCAAGTTGAATAGATTTTTAAGAATCAATTAACAAATAAATCCCAAAAAGAAAAAAAAAAACCCCACCCTCCTCTCTTCTATAAATACACACAAACCGAAACTCCAAATCCCCAATTCCCAAATCCGAAAAACAGAGCCATTTACAGAGTCCCTTCTTTGTGCAATGGCGGCCACACCATTCATCTACTTTCTCGTCCTCCTCTGCGTCTCCACCGCCGTCTCCGCCGCCGCCGCAACCCAAACCGGGTCGGTCTGCAGGTCGACCCTGGACCCGGCGTTCTGCAAAACCGCCCTCCCGAACCAAACCGGCAATGTGTACGCGTACGGGCGGTCGTCGGTGAGGAAATCGCTGTCGTCGTCGCAGAAGTTTCTGCGGACGGTGGAGAAGCAGCTCCGGGGGAGGGCGTGGCTAACGGTGGGGGCGGTGAGGGCGCTGGAGGATTGCCAGCTATTAGCCGGGTTGAACATCGATTACTTGGCGGCGGCGATGACGACGGTGGACCGGACGAGCTCGGCGTTGCCGGAGATGAAGGCGGAGGAGGTGGCGAGCCTGTTGAGTGCGCTGTTGACGAACCAGCAGACGTGTATGGATGGGCTGAAGGCGACGGCGGGTTCTTGGTCTCTCAAAAATGGACTCTCTCTCCCACTTGCTACTGACACCAAACTCTACTCTATGTCCTTGGCTCTATTTACCAAAGGTTGGGTACCGAAGAAAAAGAAAAGGTCGACATGGAAGGGGGCCGGAAAACAGGGCGGGTTCGGAAACGGCCGAATGTCGGTAAAAATGTCGAGCAGAGCCAAAGCGATCTACGAGGCGGCGGCTCGACGGAATCTGCAACAGAGCAACGACGAGGACGGCGGCGACGACCAGATATCGGTTCGGGACATCGTGGTGGTGAGCCAGGATGGGAGCGGGAATTTCACGACGATTAACGAGGCGATCGCGGCGGCGCCGAACAACTCTGCTCCGACGGAGGGGTATTTCTTGATCTTTGTGTCGGCCGGAGTTTATGAAGAGTATGTTTCGATTGCGAAGAACAAGAGGTACTTGATGATGATCGGAGATGGGATTAATCAGACCGTTATTACCGGTAATCGGAGCGTCGTTGACGGCTGGACCACTTTCAATTCCGCCACCTTTGCGGTGGTAGGCCCAGGATTCGTAGCAGTAAACATGACATTCAGAAACACAGCCGGAGCAGAAAAGCACCAAGCCGTGGCGGTCCGGAGCGGCGCCGACTTATCGACGTTCTACCTCTGCAGCTTCGAAGCCTACCAAGACACATTATACACCCACTCCCTCCGCCAGTTCTACCGCGACTGCGACATCTACGGAACCGTCGACTTCATCTTCGGCAATGCAGCCGTCGTCTTCCAAAACTGCAACATCCACCCCCGCCTTCCCATGGCCAACCAGTTCAACGCCATCACCGCCCAAGGCCGCACCGACCCGAATCAGAACACCGGCACCTCGATCCAGAACTGTCGGATCACGCCCGCCGACGACCTCGCCAATGCCACTGCTGTCAAGACCTTCCTCGGCCGGCCGTGGAAGGAGTTCTCCCGGACGGTTTATTTGCAGAATTTTATGGATGGTTTTATTAATCCGGCTGGGTGGAGGGCTTGGGATGGGGAGTTTGCTCTTAATACTTCGTATTATGCTGAGTTTGGGAATTTTGGACCGGGTTCGAACACGTCCGAGCGGGTTAATTGGACCGGGTTTCATTTGATTAATGATACTGATGCTGGGAATTTTACGGCGGCCAGTTTTGTGTTGGCCGATGATTGGCTGCCGCAGACCGGCGTTCCGTACACCAGTGGCTTGACGGAGTAGGGAAACAGGGGAATTTTGATTTTTGGAAAAAAAATTAATTTTAACTTCTTTTTTGTTAATTTAAAAAATGAGAAATTCCAAAGGCTAAATGTATAGCTTGGGATGATATGATGATATGGTTGATTCTTTTTGTTATTCCATTTTTATTTTCTGGTGAAAGAAATAGAATAAATATCTACTTTTTGTTCATGAGATTTTCTTTTTTTCAGAAATATTGAAGTCTTTTGTCTGGA

Coding sequence (CDS)

ATGGCGGCCACACCATTCATCTACTTTCTCGTCCTCCTCTGCGTCTCCACCGCCGTCTCCGCCGCCGCCGCAACCCAAACCGGGTCGGTCTGCAGGTCGACCCTGGACCCGGCGTTCTGCAAAACCGCCCTCCCGAACCAAACCGGCAATGTGTACGCGTACGGGCGGTCGTCGGTGAGGAAATCGCTGTCGTCGTCGCAGAAGTTTCTGCGGACGGTGGAGAAGCAGCTCCGGGGGAGGGCGTGGCTAACGGTGGGGGCGGTGAGGGCGCTGGAGGATTGCCAGCTATTAGCCGGGTTGAACATCGATTACTTGGCGGCGGCGATGACGACGGTGGACCGGACGAGCTCGGCGTTGCCGGAGATGAAGGCGGAGGAGGTGGCGAGCCTGTTGAGTGCGCTGTTGACGAACCAGCAGACGTGTATGGATGGGCTGAAGGCGACGGCGGGTTCTTGGTCTCTCAAAAATGGACTCTCTCTCCCACTTGCTACTGACACCAAACTCTACTCTATGTCCTTGGCTCTATTTACCAAAGGTTGGGTACCGAAGAAAAAGAAAAGGTCGACATGGAAGGGGGCCGGAAAACAGGGCGGGTTCGGAAACGGCCGAATGTCGGTAAAAATGTCGAGCAGAGCCAAAGCGATCTACGAGGCGGCGGCTCGACGGAATCTGCAACAGAGCAACGACGAGGACGGCGGCGACGACCAGATATCGGTTCGGGACATCGTGGTGGTGAGCCAGGATGGGAGCGGGAATTTCACGACGATTAACGAGGCGATCGCGGCGGCGCCGAACAACTCTGCTCCGACGGAGGGGTATTTCTTGATCTTTGTGTCGGCCGGAGTTTATGAAGAGTATGTTTCGATTGCGAAGAACAAGAGGTACTTGATGATGATCGGAGATGGGATTAATCAGACCGTTATTACCGGTAATCGGAGCGTCGTTGACGGCTGGACCACTTTCAATTCCGCCACCTTTGCGGTGGTAGGCCCAGGATTCGTAGCAGTAAACATGACATTCAGAAACACAGCCGGAGCAGAAAAGCACCAAGCCGTGGCGGTCCGGAGCGGCGCCGACTTATCGACGTTCTACCTCTGCAGCTTCGAAGCCTACCAAGACACATTATACACCCACTCCCTCCGCCAGTTCTACCGCGACTGCGACATCTACGGAACCGTCGACTTCATCTTCGGCAATGCAGCCGTCGTCTTCCAAAACTGCAACATCCACCCCCGCCTTCCCATGGCCAACCAGTTCAACGCCATCACCGCCCAAGGCCGCACCGACCCGAATCAGAACACCGGCACCTCGATCCAGAACTGTCGGATCACGCCCGCCGACGACCTCGCCAATGCCACTGCTGTCAAGACCTTCCTCGGCCGGCCGTGGAAGGAGTTCTCCCGGACGGTTTATTTGCAGAATTTTATGGATGGTTTTATTAATCCGGCTGGGTGGAGGGCTTGGGATGGGGAGTTTGCTCTTAATACTTCGTATTATGCTGAGTTTGGGAATTTTGGACCGGGTTCGAACACGTCCGAGCGGGTTAATTGGACCGGGTTTCATTTGATTAATGATACTGATGCTGGGAATTTTACGGCGGCCAGTTTTGTGTTGGCCGATGATTGGCTGCCGCAGACCGGCGTTCCGTACACCAGTGGCTTGACGGAGTAG

Protein sequence

MAATPFIYFLVLLCVSTAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVRKSLSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPEMKAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWVPKKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNAITAQGRTDPNQNTGTSIQNCRITPADDLANATAVKTFLGRPWKEFSRTVYLQNFMDGFINPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLADDWLPQTGVPYTSGLTE
Homology
BLAST of Sed0022840 vs. NCBI nr
Match: KAG6593215.1 (putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 931.8 bits (2407), Expect = 2.8e-267
Identity = 477/562 (84.88%), Postives = 513/562 (91.28%), Query Frame = 0

Query: 1   MAATPFIYFLVL-LCVSTA---VSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGR 60
           MAAT F   ++L L ++TA   +SAA+A+ + SVC ST DP++CK+ LPNQTGNVY+YGR
Sbjct: 1   MAATTFTSLILLTLYITTAQIPLSAASASASASVCTSTPDPSYCKSTLPNQTGNVYSYGR 60

Query: 61  SSVRKSLSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTS 120
           SS RKSLSSSQKFLR V+K LR R+ LTV AVRALEDCQLLAGLNIDYL  +  TV+ TS
Sbjct: 61  SSFRKSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTS 120

Query: 121 SALPEMKAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALF 180
           S L EMKA++V SLLSA+LTNQQTC+DGLKATAGSWS+KNGLSLPLA+DTKLYSMSLALF
Sbjct: 121 SKLSEMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALF 180

Query: 181 TKGWVPKKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQ 240
           TKGWVPKKKKRSTW  AG QGGF NGRMS+KMS+RAKAIYE A RRNL ++  +DGGDDQ
Sbjct: 181 TKGWVPKKKKRSTWTAAGSQGGFRNGRMSLKMSNRAKAIYEMATRRNLLET--DDGGDDQ 240

Query: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYL 300
           IS+RDIV+VSQDGSGNFTTINEAIAAAPNNSAPT+GYFLIFVS GVYEEYVSIAKNKRYL
Sbjct: 241 ISIRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYL 300

Query: 301 MMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRS 360
           MMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGA KHQAVAVR+
Sbjct: 301 MMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMA 420
           GADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI+PRLPMA
Sbjct: 361 GADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIQNCRITPADDLANAT--AVKTFLGRPWKEFSRTVYLQN 480
           NQFNAITAQGRTDPNQNTGTSI NCRIT ADDLAN T  AVKTFLGRPWKE+SRTVYLQ+
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQS 480

Query: 481 FMDGFINPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAA 540
           FMD  INPAGWRAWD +FALNTSYYAEFGNFGPGSNTSERVNW GFHLINDTDAGNFTAA
Sbjct: 481 FMDELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAA 540

Query: 541 SFVLADDWLPQTGVPYTSGLTE 557
           +FVLADDWLPQTGVPYTSGLTE
Sbjct: 541 NFVLADDWLPQTGVPYTSGLTE 560

BLAST of Sed0022840 vs. NCBI nr
Match: KAG7030208.1 (putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 929.5 bits (2401), Expect = 1.4e-266
Identity = 475/560 (84.82%), Postives = 511/560 (91.25%), Query Frame = 0

Query: 1   MAATPFIYFLVL-LCVSTA-VSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSS 60
           MAAT F   ++L L ++TA +  +AA+ + SVC ST DP++CK+ LPNQTGNVY+YGRSS
Sbjct: 1   MAATTFTSLILLTLYITTAQIPLSAASASASVCTSTPDPSYCKSTLPNQTGNVYSYGRSS 60

Query: 61  VRKSLSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSA 120
            RKSLSSSQKFLR V+K LR R+ LTV AVRALEDCQLLAGLNIDYL  +  TV+ TSS 
Sbjct: 61  FRKSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSK 120

Query: 121 LPEMKAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTK 180
           L EMKA++V SLLSA+LTNQQTC+DGLKATAGSWS+KNGLSLPLA+DTKLYSMSLALFTK
Sbjct: 121 LSEMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTK 180

Query: 181 GWVPKKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQIS 240
           GWVPKKKKRSTW  AG QGGF NGRMS+KMS+RAKAIYE A RRNL ++  +DGGDDQIS
Sbjct: 181 GWVPKKKKRSTWTAAGSQGGFRNGRMSLKMSNRAKAIYEMATRRNLLET--DDGGDDQIS 240

Query: 241 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMM 300
           +RDIV+VSQDGSGNFTTINEAIAAAPNNSAPT+GYFLIFVS GVYEEYVSIAKNKRYLMM
Sbjct: 241 IRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMM 300

Query: 301 IGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGA 360
           IGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGA KHQAVAVR+GA
Sbjct: 301 IGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360

Query: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQ 420
           DLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI+PRLPMANQ
Sbjct: 361 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIQNCRITPADDLANAT--AVKTFLGRPWKEFSRTVYLQNFM 480
           FNAITAQGRTDPNQNTGTSI NCRIT ADDLAN T  AVKTFLGRPWKE+SRTVYLQ+FM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFM 480

Query: 481 DGFINPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASF 540
           D  INPAGWRAWD +FALNTSYYAEFGNFGPGSNTSERVNW GFHLINDTDAGNFTAA+F
Sbjct: 481 DELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANF 540

Query: 541 VLADDWLPQTGVPYTSGLTE 557
           VLADDWLPQTGVPYTSGLTE
Sbjct: 541 VLADDWLPQTGVPYTSGLTE 558

BLAST of Sed0022840 vs. NCBI nr
Match: XP_022959747.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata])

HSP 1 Score: 928.3 bits (2398), Expect = 3.1e-266
Identity = 471/558 (84.41%), Postives = 503/558 (90.14%), Query Frame = 0

Query: 1   MAATPFIYFLVLLCVSTAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVR 60
           MAAT F   ++L    T      +    SVC ST DP++CK+ LPNQTGNVY+YGRSS R
Sbjct: 1   MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60

Query: 61  KSLSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALP 120
           KSLSSSQKFLR V+K LR R+ LTV AVRALEDCQLLAGLNIDYL  +  TV+ TSS L 
Sbjct: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120

Query: 121 EMKAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGW 180
           EMKA++V SLLSA+LTNQQTC+DGLKATAGSWS+KNGLSLPLA+DTKLYSMSLALFTKGW
Sbjct: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180

Query: 181 VPKKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVR 240
           VPKKKKRSTW  AG+QGGF NGRMS+KMS+RAKAIYE A RRNL ++  +DGGDDQIS+R
Sbjct: 181 VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLET--DDGGDDQISIR 240

Query: 241 DIVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIG 300
           DIV+VSQDGSGNFTTINEAIAAAPNNSAPT+GYFLIFVS GVYEEYVSIAKNKRYLMMIG
Sbjct: 241 DIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIG 300

Query: 301 DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADL 360
           DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGA KHQAVAVR+GADL
Sbjct: 301 DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADL 360

Query: 361 STFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFN 420
           STFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI+PRLPMANQFN
Sbjct: 361 STFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFN 420

Query: 421 AITAQGRTDPNQNTGTSIQNCRITPADDLANAT--AVKTFLGRPWKEFSRTVYLQNFMDG 480
           AITAQGRTDPNQNTGTSI NCRIT ADDLAN T  AVKTFLGRPWKE+SRTVYLQ+FMD 
Sbjct: 421 AITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDE 480

Query: 481 FINPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVL 540
            INPAGWRAWD +FALNTSYYAEFGNFGPGSNTSERVNW GFHLINDTDAGNFTAA+FVL
Sbjct: 481 LINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVL 540

Query: 541 ADDWLPQTGVPYTSGLTE 557
           ADDWLPQTGVPYTSGLTE
Sbjct: 541 ADDWLPQTGVPYTSGLTE 556

BLAST of Sed0022840 vs. NCBI nr
Match: XP_038896831.1 (probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida])

HSP 1 Score: 927.2 bits (2395), Expect = 6.8e-266
Identity = 467/556 (83.99%), Postives = 502/556 (90.29%), Query Frame = 0

Query: 3   ATPFIYFLVLLCVSTAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVRKS 62
           A    YFL+L+ +    SAA    TGS C ST DP++CK+ LPNQTGNVY+YGRSS RKS
Sbjct: 11  AAAAFYFLILISLYITTSAA---PTGSECSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKS 70

Query: 63  LSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPEM 122
           LS+SQKFLR VEK LR R+ LTV AVRALEDCQLLAGLNIDYL  +   V+ TS  LPEM
Sbjct: 71  LSASQKFLRLVEKHLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEM 130

Query: 123 KAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWVP 182
           KA++V SLLSA+LTNQQTC+DG+KATAGSWSLKNGLSLPLA DTKLYS+SLA FTKGWVP
Sbjct: 131 KADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVP 190

Query: 183 KKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVRDI 242
           KKKK+ TWK AG+ GGF NGRMS+KMSSRAKAIYE A RRNL Q+  +DGGDDQI+VRDI
Sbjct: 191 KKKKKPTWKAAGRHGGFRNGRMSLKMSSRAKAIYETATRRNLLQA--DDGGDDQIAVRDI 250

Query: 243 VVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDG 302
           VVVSQDGSGNFTTINEA+AAAPNNSAPT+GYFLIFVSAGVYEEYVSIAKNKRYLMMIGDG
Sbjct: 251 VVVSQDGSGNFTTINEAVAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDG 310

Query: 303 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLST 362
           INQTV+TGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGA KHQAVAVR+GADLST
Sbjct: 311 INQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 370

Query: 363 FYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNAI 422
           FYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI+PRLPM+NQFNAI
Sbjct: 371 FYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAI 430

Query: 423 TAQGRTDPNQNTGTSIQNCRITPADDLANAT--AVKTFLGRPWKEFSRTVYLQNFMDGFI 482
           TAQGRTDPNQNTGTSI NCRIT ADDLAN T   VKTFLGRPWKE+SRTVY+Q+FMD  I
Sbjct: 431 TAQGRTDPNQNTGTSIYNCRITAADDLANNTGAGVKTFLGRPWKEYSRTVYMQSFMDDLI 490

Query: 483 NPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLAD 542
           NPAGWRAWDG+FALNTSYYAEFGNFGPGSNTSERVNW GFHLINDTDAGNFTA +FVLAD
Sbjct: 491 NPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLAD 550

Query: 543 DWLPQTGVPYTSGLTE 557
           DWLPQTGVPYTSGLT+
Sbjct: 551 DWLPQTGVPYTSGLTD 561

BLAST of Sed0022840 vs. NCBI nr
Match: XP_023513640.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 925.2 bits (2390), Expect = 2.6e-265
Identity = 471/558 (84.41%), Postives = 501/558 (89.78%), Query Frame = 0

Query: 1   MAATPFIYFLVLLCVSTAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVR 60
           MAAT F   ++L    T      +  + SVC ST DP++CK+ LPNQTGNVY+YGRSS R
Sbjct: 1   MAATTFSSLILLSLYITTAQIPLSAASASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60

Query: 61  KSLSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALP 120
           KSLSSSQKFLR V+K LR R+ LTV AVRALEDCQLLAGLNIDYL  +  TV+ TSS L 
Sbjct: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120

Query: 121 EMKAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGW 180
           EMKA++V SLLSA+LTNQQTC+DGLKATAGSWS+KNGL+LPLA+DTKLYSMSLALFTKGW
Sbjct: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLALPLASDTKLYSMSLALFTKGW 180

Query: 181 VPKKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVR 240
           VPKKKKRSTW  AG QGGF NGRMS+KMSSRAKAIYE A RRNL Q+  E GGDDQIS+R
Sbjct: 181 VPKKKKRSTWTAAGSQGGFRNGRMSLKMSSRAKAIYEMATRRNLLQT--EGGGDDQISIR 240

Query: 241 DIVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIG 300
           DIV+VSQDGSGNFTTINEAIAAAPNNSAPT+GYFLIFVS GVYEEYVSIAKNKRYLMMIG
Sbjct: 241 DIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIG 300

Query: 301 DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADL 360
           DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGA KHQAVAVR+GADL
Sbjct: 301 DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADL 360

Query: 361 STFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFN 420
           STFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI+PRLPMANQFN
Sbjct: 361 STFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFN 420

Query: 421 AITAQGRTDPNQNTGTSIQNCRITPADDLANAT--AVKTFLGRPWKEFSRTVYLQNFMDG 480
           AITAQGRTDPNQNTGTSI NCRIT ADDLAN T   VKTFLGRPWKE+SRTVYLQ+FMD 
Sbjct: 421 AITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVTVKTFLGRPWKEYSRTVYLQSFMDE 480

Query: 481 FINPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVL 540
            INPAGWRAWD +FALNTSYYAEFGNFGPGSNTSERVNW GFHLINDTDAGNFTAA+FVL
Sbjct: 481 LINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVL 540

Query: 541 ADDWLPQTGVPYTSGLTE 557
           ADDWLPQTGVPYTSGLTE
Sbjct: 541 ADDWLPQTGVPYTSGLTE 556

BLAST of Sed0022840 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 1.0e-195
Identity = 344/573 (60.03%), Postives = 434/573 (75.74%), Query Frame = 0

Query: 7   IYFLVLLCV---STAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVRKSL 66
           I+ L+ L     S   S+   + + ++C++T DP +CK+  P+  GNV  YG  S+RKSL
Sbjct: 5   IFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSL 64

Query: 67  SSSQKFLRTVEKQLRGRAWLTVGAV-RALEDCQLLAGLNIDYLAAAMTTVDRTSS----- 126
           S S+KF+RTV++ ++  A L+  AV RAL+DC+ LAGL +DYL  +  TV+ TS+     
Sbjct: 65  SQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFK 124

Query: 127 ALPEMKAEEVASLLSALLTNQQTCMDGLKATA---GSWSLKNGLSLPLATDTKLYSMSLA 186
            L   KA+++ +LLSA LTN+QTC++GL   A    +W+++ G++LPL  DTKL  +SLA
Sbjct: 125 PLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLA 184

Query: 187 LFTKGWVPKKKKRSTWKGAGKQGG----------FGNGRMSVKMSSRAKAIYEAAARRNL 246
           LFTKGWVPKKKKR+ +  A  + G          F NG + +KM+ + KA+YE+ +RR L
Sbjct: 185 LFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKL 244

Query: 247 QQSNDEDGGDD--QISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGV 306
              +    GDD   + + DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+AG+
Sbjct: 245 ADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGI 304

Query: 307 YEEYVSIAKNKRYLMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRN 366
           YEEY+SIAKNKRY+MMIGDGINQTV+TGNRSVVDGWTTFNSATFAV  P FVAVN+TFRN
Sbjct: 305 YEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRN 364

Query: 367 TAGAEKHQAVAVRSGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAV 426
           TAG EKHQAVA+RSGAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAV
Sbjct: 365 TAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAV 424

Query: 427 VFQNCNIHPRLPMANQFNAITAQGRTDPNQNTGTSIQNCRITPADDLANAT-AVKTFLGR 486
           VFQNCN++PR PM NQFNAITAQGR+DPNQNTGTSIQNC I PADDL ++   VKT+LGR
Sbjct: 425 VFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGR 484

Query: 487 PWKEFSRTVYLQNFMDGFINPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFH 546
           PWKE+SRTVY+Q+++DGF+ P GWR W+G+FAL+T YYAE+ N GPGSNT+ RV W G+H
Sbjct: 485 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 544

Query: 547 LINDTDAGNFTAASFVLADDWLPQTGVPYTSGL 555
           +IN TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 545 VINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of Sed0022840 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 654.4 bits (1687), Expect = 1.1e-186
Identity = 331/547 (60.51%), Postives = 420/547 (76.78%), Query Frame = 0

Query: 24  ATQTGSVCRSTLDPAFCKTALPNQT-GNVYAYGRSSVRKSLSSSQKFLRTVEKQL-RGRA 83
           +  + ++C++T DP FCK+  P  + G+V  YGR S+RKSL+ S+KF RT+++ L R  A
Sbjct: 28  SNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQSRKFTRTIDRYLKRNNA 87

Query: 84  WLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSA--LPEMKAEEVASLLSALLTNQQ 143
            L+  AV AL+DC+ LA L  DYL  +  TV+ T+S+  L   KA+E+ +LLSA LTN+Q
Sbjct: 88  LLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSKADEIQTLLSAALTNEQ 147

Query: 144 TCMDGLK-ATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWVPKKKK----------RS 203
           TC+DG+  A + SW+++NG++LPL  DTKL+S+SLALFTKGWVPKKKK          ++
Sbjct: 148 TCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVPKKKKQVASYSWAHPKN 207

Query: 204 TWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVRDIVVVSQD 263
           T         F NG + +KM+   +A+YE+ +RR L    D+D   + + V DIV V+Q+
Sbjct: 208 THSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLA---DDDNDVNTVLVSDIVTVNQN 267

Query: 264 GSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVI 323
           G+GNFTTI EA+ +APN +  T GYF+I+V++GVYEE V IAKNKRYLMMIGDGIN+TV+
Sbjct: 268 GTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDGINRTVV 327

Query: 324 TGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLSTFYLCSF 383
           TGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG EKHQAVA+RS ADLS FY CSF
Sbjct: 328 TGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSF 387

Query: 384 EAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNAITAQGRT 443
           EAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN++PR PM NQFNAITAQGRT
Sbjct: 388 EAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRT 447

Query: 444 DPNQNTGTSIQNCRITPADDLANAT-AVKTFLGRPWKEFSRTVYLQNFMDGFINPAGWRA 503
           DPNQNTG SI NC I PADDL ++   VKT+LGRPWKE+SRTV++Q+++D  + P GWR 
Sbjct: 448 DPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWRE 507

Query: 504 WDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLADDWLPQTG 555
           W+G+FAL+T YYAE+ N G GS+T++RV W G+H+IN TDA NFT  +F+L D W+ Q+G
Sbjct: 508 WNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDGWMVQSG 567

BLAST of Sed0022840 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 633.6 bits (1633), Expect = 2.0e-180
Identity = 315/537 (58.66%), Postives = 404/537 (75.23%), Query Frame = 0

Query: 24  ATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVRKSLSSSQKFLRTVEKQLRGRAWL 83
           AT   ++CR   DP++C++ LPNQ G++Y+YGR S+R+SLS +++F+  ++ +L  +  +
Sbjct: 32  ATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKV 91

Query: 84  TV-GAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPEMKAEEVASLLSALLTNQQTCM 143
                V ALEDC+ LA L +DYL ++  T D T + L   +AE+V + LSA +TN+QTC+
Sbjct: 92  AAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKT-LSLSRAEDVHTFLSAAITNEQTCL 151

Query: 144 DGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWVPKKKK-RSTWKGAG---KQGG 203
           +GLK+TA     +NGLS  L  DTKLY +SLALF+KGWVP++++ R  W+      K  G
Sbjct: 152 EGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFG 211

Query: 204 FGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVRDIVVVSQDGSGNFTTINE 263
           F NG++ +KM+ RA+A+Y    RR L QS+      D + V DIV V Q+G+GNFTTIN 
Sbjct: 212 FRNGKLPLKMTERARAVYNTVTRRKLLQSD-----ADAVQVSDIVTVIQNGTGNFTTINA 271

Query: 264 AIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVITGNRSVVDGW 323
           AIAAAPN +  + GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTVITGNRSVVDGW
Sbjct: 272 AIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGW 331

Query: 324 TTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLSTFYLCSFEAYQDTLYTH 383
           TTFNSATF + GP F+ VN+T RNTAG  K QAVA+RSG DLS FY CSFEAYQDTLYTH
Sbjct: 332 TTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTH 391

Query: 384 SLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNAITAQGRTDPNQNTGTSI 443
           SLRQFYR+CD+YGTVDFIFGNAAVV QNCN++PR P   Q N +TAQGRTDPNQNTGT+I
Sbjct: 392 SLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAI 451

Query: 444 QNCRITPADDLANAT-AVKTFLGRPWKEFSRTVYLQNFMDGFINPAGWRAWDGEFALNTS 503
             C I PADDLA +   VKT+LGRPWKE+SRTV +Q ++DGF+ P+GW AW G+FAL+T 
Sbjct: 452 HGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTL 511

Query: 504 YYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLADDWLPQTGVPYTSGL 555
           YYAE+ N GPGS+T+ RV W G+H+IN TDA NFT  +F++ + W+ QTGVP+  GL
Sbjct: 512 YYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of Sed0022840 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 9.3e-133
Identity = 260/541 (48.06%), Postives = 352/541 (65.06%), Query Frame = 0

Query: 31  CRSTLDPAFCKT---ALPNQTGNVYAYGRSSVRKSLSSSQKFLRTVEKQLR----GRAWL 90
           C+ST  P  C+T   A+ +   + Y YG+ ++++ L  +++  + + +  +         
Sbjct: 88  CKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEADPGTS 147

Query: 91  TVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPEMKAEEVASLLSALLTNQQTCMD 150
           TV  V A+ DC  LA L+++YL   +T   + +  +     + V SLL  ++TNQQTC+D
Sbjct: 148 TVEEVSAVADCGELAELSVEYL-ETVTEELKAAELMTAALVDRVTSLLGGVVTNQQTCLD 207

Query: 151 GLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWVPKKKKRSTWKGA-GKQGGFGNG 210
           GL      ++    +  PL   T+LYS+SL L +   + +  KR  +KG+ GK  G GN 
Sbjct: 208 GLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKR--YKGSKGKIFGGGNK 267

Query: 211 RMSVKMSSRAKAIYEAA--------ARRNLQQSNDEDGGDDQISVRDIVVVSQDGSGNFT 270
            +   + +  K + +          A RNL +  +  GG   I VR+ V V    + NF 
Sbjct: 268 PVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGG--SILVREAVTVGPYETDNFP 327

Query: 271 TINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVITGNRSV 330
           TI EA+AAAPN++ P +GYF+I+  AG+YEEYV I+  KR +M+IGDGIN+T+I+GN S 
Sbjct: 328 TITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNHSF 387

Query: 331 VDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLSTFYLCSFEAYQDT 390
           +DGWTT+NS+TFAVVG  FVAV++TFRNTAG EKHQAVAVR+ AD STFY CSFE YQDT
Sbjct: 388 IDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQDT 447

Query: 391 LYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNAITAQGRTDPNQNT 450
           LY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNI+ R PMANQ NA+TA GRTDPNQ T
Sbjct: 448 LYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQKT 507

Query: 451 GTSIQNCRITPADDL-ANATAVKTFLGRPWKEFSRTVYLQNFMDGFINPAGWRAWDGEFA 510
           G SI NC I  A DL A+  +  TFLGRPWK +SRTVY+Q+++   + P GW  W+G   
Sbjct: 508 GISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNGTTG 567

Query: 511 LNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLADDWLPQTGVPYTSG 555
           L+T  Y E+ NFGPG++TS+RV W+G+ L+N   A NFT  +F L D WLPQT +P+  G
Sbjct: 568 LDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDIPFYGG 620

BLAST of Sed0022840 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 2.7e-132
Identity = 256/542 (47.23%), Postives = 346/542 (63.84%), Query Frame = 0

Query: 31  CRSTLDPAFCKT---ALPNQTGNVYAYGRSSVRKSLSSSQKFLRTVEKQLR------GRA 90
           C+ST  P  C+T   A+ +   + Y YG+ ++++ L  + +  + +    R      G A
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 91  WLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPEMKAEEVASLLSALLTNQQTC 150
             T   + A+ DC  L+ L+++YL   +TT  +T+  +     E V SLLS ++TNQQTC
Sbjct: 143 --TAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSLLSGVVTNQQTC 202

Query: 151 MDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWVPKKKKRSTWKGAGKQGGFGN 210
           +DGL      ++    +  P+   T+LYS+SL L +       K+    K  GK  G GN
Sbjct: 203 LDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASK--GKILGGGN 262

Query: 211 GRMSVKMSSRAKAIYEAA--------ARRNLQQSNDEDGGDDQISVRDIVVVSQDGSGNF 270
                 + +  K + +            RNL +  +  GG   I V   V+V    S NF
Sbjct: 263 STYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGG--SILVSKAVIVGPFKSDNF 322

Query: 271 TTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVITGNRS 330
           TTI +AIAAAPNN+ P +GYF+I+   GVYEEY+ +  NK+ LM++GDGIN+T+ITGN +
Sbjct: 323 TTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHN 382

Query: 331 VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLSTFYLCSFEAYQD 390
           VVDGWTT+N ++FAVVG  F+AV++TFRNTAG EKHQAVA+R+ A+ S+FY CSFE YQD
Sbjct: 383 VVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQD 442

Query: 391 TLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNAITAQGRTDPNQN 450
           TLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNI+ R PMA Q NAITA GR DPNQN
Sbjct: 443 TLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQN 502

Query: 451 TGTSIQNCRITPADDL-ANATAVKTFLGRPWKEFSRTVYLQNFMDGFINPAGWRAWDGEF 510
           TG SI NC I  A DL A   +  TFLGRPWK +SRTV++Q+++   + P GW  W+G  
Sbjct: 503 TGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTI 562

Query: 511 ALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLADDWLPQTGVPYTS 555
            L+T YY E+ NFGPG+NT++RV W G++L+N  +A NFT  +F + D WLPQT +P+  
Sbjct: 563 GLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIPFYG 615

BLAST of Sed0022840 vs. ExPASy TrEMBL
Match: A0A6J1H5E4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460725 PE=3 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.5e-266
Identity = 471/558 (84.41%), Postives = 503/558 (90.14%), Query Frame = 0

Query: 1   MAATPFIYFLVLLCVSTAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVR 60
           MAAT F   ++L    T      +    SVC ST DP++CK+ LPNQTGNVY+YGRSS R
Sbjct: 1   MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60

Query: 61  KSLSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALP 120
           KSLSSSQKFLR V+K LR R+ LTV AVRALEDCQLLAGLNIDYL  +  TV+ TSS L 
Sbjct: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120

Query: 121 EMKAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGW 180
           EMKA++V SLLSA+LTNQQTC+DGLKATAGSWS+KNGLSLPLA+DTKLYSMSLALFTKGW
Sbjct: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180

Query: 181 VPKKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVR 240
           VPKKKKRSTW  AG+QGGF NGRMS+KMS+RAKAIYE A RRNL ++  +DGGDDQIS+R
Sbjct: 181 VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLET--DDGGDDQISIR 240

Query: 241 DIVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIG 300
           DIV+VSQDGSGNFTTINEAIAAAPNNSAPT+GYFLIFVS GVYEEYVSIAKNKRYLMMIG
Sbjct: 241 DIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIG 300

Query: 301 DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADL 360
           DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGA KHQAVAVR+GADL
Sbjct: 301 DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADL 360

Query: 361 STFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFN 420
           STFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI+PRLPMANQFN
Sbjct: 361 STFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFN 420

Query: 421 AITAQGRTDPNQNTGTSIQNCRITPADDLANAT--AVKTFLGRPWKEFSRTVYLQNFMDG 480
           AITAQGRTDPNQNTGTSI NCRIT ADDLAN T  AVKTFLGRPWKE+SRTVYLQ+FMD 
Sbjct: 421 AITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDE 480

Query: 481 FINPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVL 540
            INPAGWRAWD +FALNTSYYAEFGNFGPGSNTSERVNW GFHLINDTDAGNFTAA+FVL
Sbjct: 481 LINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVL 540

Query: 541 ADDWLPQTGVPYTSGLTE 557
           ADDWLPQTGVPYTSGLTE
Sbjct: 541 ADDWLPQTGVPYTSGLTE 556

BLAST of Sed0022840 vs. ExPASy TrEMBL
Match: A0A5D3D3H4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001320 PE=3 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.3e-265
Identity = 464/557 (83.30%), Postives = 505/557 (90.66%), Query Frame = 0

Query: 2   AATPFIYFLVLLCVSTAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVRK 61
           A T    F VL+ +S  ++  +A+ TGSVC ST DP++CK+ALPNQTGNVY+YGRSS RK
Sbjct: 3   AGTTAAAFYVLILISLYIT-TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRK 62

Query: 62  SLSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPE 121
           SLSSSQKFLR VEK LR R+ LTV AVRALEDCQLLAGLNIDYL  +   V+ TS AL E
Sbjct: 63  SLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRALTE 122

Query: 122 MKAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWV 181
           MKA++V SLLSA+LTNQQTC+DG+KATAGSWSLKNGLS PLA+DTKLYS+SLA FTKGWV
Sbjct: 123 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWV 182

Query: 182 PKKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVRD 241
           PKKKK+ TWK AG+QGGF NGRMS+KMSSR +AIYE A RRNL Q++D+ G DDQI+VRD
Sbjct: 183 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIAVRD 242

Query: 242 IVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 301
           IVVVSQDG+GNFTTINEAIAAAPNNSAPT+GYFLIFVSAGVYEEYVSIAKNKRYLMMIGD
Sbjct: 243 IVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 302

Query: 302 GINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLS 361
           GINQT++TGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGA KHQAVA+R+GADLS
Sbjct: 303 GINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGADLS 362

Query: 362 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNA 421
           TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI+PRLPM+NQFNA
Sbjct: 363 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 422

Query: 422 ITAQGRTDPNQNTGTSIQNCRITPADDLAN--ATAVKTFLGRPWKEFSRTVYLQNFMDGF 481
           ITAQGRTDPNQNTGTSI NCRIT ADDLAN     VKTFLGRPWKE+SRTVY+Q+FMD  
Sbjct: 423 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 482

Query: 482 INPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLA 541
           INPAGWRAWDG+FALNTSYYAEFGNFGPGSNTSERVNW GFHLINDTDAGNFTA +FVLA
Sbjct: 483 INPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 542

Query: 542 DDWLPQTGVPYTSGLTE 557
           DDWLPQTGVPYTSGLTE
Sbjct: 543 DDWLPQTGVPYTSGLTE 558

BLAST of Sed0022840 vs. ExPASy TrEMBL
Match: A0A1S3BSI2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.3e-265
Identity = 464/557 (83.30%), Postives = 505/557 (90.66%), Query Frame = 0

Query: 2   AATPFIYFLVLLCVSTAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVRK 61
           A T    F VL+ +S  ++  +A+ TGSVC ST DP++CK+ALPNQTGNVY+YGRSS RK
Sbjct: 3   AGTTAAAFYVLILISLYIT-TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRK 62

Query: 62  SLSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPE 121
           SLSSSQKFLR VEK LR R+ LTV AVRALEDCQLLAGLNIDYL  +   V+ TS AL E
Sbjct: 63  SLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRALTE 122

Query: 122 MKAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWV 181
           MKA++V SLLSA+LTNQQTC+DG+KATAGSWSLKNGLS PLA+DTKLYS+SLA FTKGWV
Sbjct: 123 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWV 182

Query: 182 PKKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVRD 241
           PKKKK+ TWK AG+QGGF NGRMS+KMSSR +AIYE A RRNL Q++D+ G DDQI+VRD
Sbjct: 183 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIAVRD 242

Query: 242 IVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 301
           IVVVSQDG+GNFTTINEAIAAAPNNSAPT+GYFLIFVSAGVYEEYVSIAKNKRYLMMIGD
Sbjct: 243 IVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 302

Query: 302 GINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLS 361
           GINQT++TGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGA KHQAVA+R+GADLS
Sbjct: 303 GINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGADLS 362

Query: 362 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNA 421
           TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI+PRLPM+NQFNA
Sbjct: 363 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 422

Query: 422 ITAQGRTDPNQNTGTSIQNCRITPADDLAN--ATAVKTFLGRPWKEFSRTVYLQNFMDGF 481
           ITAQGRTDPNQNTGTSI NCRIT ADDLAN     VKTFLGRPWKE+SRTVY+Q+FMD  
Sbjct: 423 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 482

Query: 482 INPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLA 541
           INPAGWRAWDG+FALNTSYYAEFGNFGPGSNTSERVNW GFHLINDTDAGNFTA +FVLA
Sbjct: 483 INPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 542

Query: 542 DDWLPQTGVPYTSGLTE 557
           DDWLPQTGVPYTSGLTE
Sbjct: 543 DDWLPQTGVPYTSGLTE 558

BLAST of Sed0022840 vs. ExPASy TrEMBL
Match: A0A6J1KSC0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111497795 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 6.9e-264
Identity = 472/559 (84.44%), Postives = 506/559 (90.52%), Query Frame = 0

Query: 1   MAATPFIYFLVL-LCVSTAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSV 60
           MAAT F   ++L L ++TA    +A    SVC ST DP++CK+ALPNQTGNVY+YGRSS 
Sbjct: 1   MAATTFSSLILLSLYITTAQIPLSA---ASVCTSTPDPSYCKSALPNQTGNVYSYGRSSF 60

Query: 61  RKSLSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSAL 120
           RKSLSSSQKFLR V+K LR R+ LTV AV ALEDC+LLAGLNIDYL  +   V+ TSS L
Sbjct: 61  RKSLSSSQKFLRLVKKYLRSRSSLTVPAVGALEDCKLLAGLNIDYLTTSFRAVNTTSSKL 120

Query: 121 PEMKAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKG 180
           PEMKA++V SLLSA+LTNQQTC+DGLKATAGSWS+KNGLSLPLA+DTKLYSMSLALFTKG
Sbjct: 121 PEMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKG 180

Query: 181 WVPKKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISV 240
           WVPKKKKRSTW  AG  GGF NGRMS+KMS+RAKAIYE A RRNL ++  +DGGDDQIS+
Sbjct: 181 WVPKKKKRSTWTAAGSLGGFRNGRMSLKMSNRAKAIYEMATRRNLLET--DDGGDDQISI 240

Query: 241 RDIVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMI 300
           RDIV+VSQDGSGNFTTINEAIAAAPNNSAPT+GYFLIFVS GVYEEYVSIAKNKRYLMMI
Sbjct: 241 RDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMI 300

Query: 301 GDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGAD 360
           GDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGA KHQAVAVR+GAD
Sbjct: 301 GDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGAD 360

Query: 361 LSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQF 420
           LSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI+PRLPMANQF
Sbjct: 361 LSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQF 420

Query: 421 NAITAQGRTDPNQNTGTSIQNCRITPADDLANAT--AVKTFLGRPWKEFSRTVYLQNFMD 480
           NAITAQGRTDPNQNTGTSI NCRIT ADDLAN T  AVKTFLGRPWKE+SRTVYLQ+FMD
Sbjct: 421 NAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMD 480

Query: 481 GFINPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFV 540
             INPAGWRAWD +FALNTSYYAEFGNFGPGSNTSERVNW GFHLINDTDAGNFTAA+FV
Sbjct: 481 ELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFV 540

Query: 541 LADDWLPQTGVPYTSGLTE 557
           LADDWLPQTGVPYTSGLTE
Sbjct: 541 LADDWLPQTGVPYTSGLTE 554

BLAST of Sed0022840 vs. ExPASy TrEMBL
Match: A0A6J1JVJ5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.5e-263
Identity = 463/557 (83.12%), Postives = 500/557 (89.77%), Query Frame = 0

Query: 4   TPFIYFLVL--LCVSTAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVRK 63
           TP  Y+L+L  L ++ A  + +A  TGSVC S  DP++CK+ALPNQTGNVY+YGRSSVRK
Sbjct: 8   TPAFYYLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVRK 67

Query: 64  SLSSSQKFLRTVEKQLRGRAWLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPE 123
           SLSSSQKFLR VEK LR R+ LTV AVR+LEDCQLLAGLNIDYL  +  TV+ TS  LPE
Sbjct: 68  SLSSSQKFLRLVEKYLRSRSSLTVAAVRSLEDCQLLAGLNIDYLTTSFRTVNSTSWTLPE 127

Query: 124 MKAEEVASLLSALLTNQQTCMDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWV 183
           MKAE+V SLLSA+LTNQQTC+DGLKATAGSWSLKNGLSLPL  DTKLYSMSLA FTKGWV
Sbjct: 128 MKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLDADTKLYSMSLAFFTKGWV 187

Query: 184 PKKKKRSTWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVRD 243
           PKKKK+ TW  AG+QGGF NGRMS KMSSR KAIYE A RRNL Q+  +DGGDDQISVRD
Sbjct: 188 PKKKKKQTWTSAGRQGGFSNGRMSFKMSSRTKAIYEMATRRNLLQT--DDGGDDQISVRD 247

Query: 244 IVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 303
           IVVVSQDGSGNFTTINEAIAAAPNNSAPT+GYFLIFV AGVYEEYV+IAKNKRYLMMIGD
Sbjct: 248 IVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGD 307

Query: 304 GINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLS 363
           GINQTV+TGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGA KHQAVAVR+ ADLS
Sbjct: 308 GINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLS 367

Query: 364 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNA 423
           TFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNI+PRLPMANQFNA
Sbjct: 368 TFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNA 427

Query: 424 ITAQGRTDPNQNTGTSIQNCRITPADDLANATA--VKTFLGRPWKEFSRTVYLQNFMDGF 483
           ITAQGRTDPNQNTGTSI NCRIT ADDLAN T   V TFLGRPWKE+SRTVY+Q+FM+  
Sbjct: 428 ITAQGRTDPNQNTGTSIHNCRITAADDLANNTGARVNTFLGRPWKEYSRTVYMQSFMNDL 487

Query: 484 INPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLA 543
           INPAGWRAWDG+FALNTSYYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNFTA +F++A
Sbjct: 488 INPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAGNFLMA 547

Query: 544 DDWLPQTGVPYTSGLTE 557
           DDW+PQTGVPYTSGLTE
Sbjct: 548 DDWVPQTGVPYTSGLTE 562

BLAST of Sed0022840 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 684.5 bits (1765), Expect = 7.2e-197
Identity = 344/573 (60.03%), Postives = 434/573 (75.74%), Query Frame = 0

Query: 7   IYFLVLLCV---STAVSAAAATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVRKSL 66
           I+ L+ L     S   S+   + + ++C++T DP +CK+  P+  GNV  YG  S+RKSL
Sbjct: 5   IFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSL 64

Query: 67  SSSQKFLRTVEKQLRGRAWLTVGAV-RALEDCQLLAGLNIDYLAAAMTTVDRTSS----- 126
           S S+KF+RTV++ ++  A L+  AV RAL+DC+ LAGL +DYL  +  TV+ TS+     
Sbjct: 65  SQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFK 124

Query: 127 ALPEMKAEEVASLLSALLTNQQTCMDGLKATA---GSWSLKNGLSLPLATDTKLYSMSLA 186
            L   KA+++ +LLSA LTN+QTC++GL   A    +W+++ G++LPL  DTKL  +SLA
Sbjct: 125 PLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLA 184

Query: 187 LFTKGWVPKKKKRSTWKGAGKQGG----------FGNGRMSVKMSSRAKAIYEAAARRNL 246
           LFTKGWVPKKKKR+ +  A  + G          F NG + +KM+ + KA+YE+ +RR L
Sbjct: 185 LFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKL 244

Query: 247 QQSNDEDGGDD--QISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGV 306
              +    GDD   + + DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+AG+
Sbjct: 245 ADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGI 304

Query: 307 YEEYVSIAKNKRYLMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRN 366
           YEEY+SIAKNKRY+MMIGDGINQTV+TGNRSVVDGWTTFNSATFAV  P FVAVN+TFRN
Sbjct: 305 YEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRN 364

Query: 367 TAGAEKHQAVAVRSGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAV 426
           TAG EKHQAVA+RSGAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAV
Sbjct: 365 TAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAV 424

Query: 427 VFQNCNIHPRLPMANQFNAITAQGRTDPNQNTGTSIQNCRITPADDLANAT-AVKTFLGR 486
           VFQNCN++PR PM NQFNAITAQGR+DPNQNTGTSIQNC I PADDL ++   VKT+LGR
Sbjct: 425 VFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGR 484

Query: 487 PWKEFSRTVYLQNFMDGFINPAGWRAWDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFH 546
           PWKE+SRTVY+Q+++DGF+ P GWR W+G+FAL+T YYAE+ N GPGSNT+ RV W G+H
Sbjct: 485 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 544

Query: 547 LINDTDAGNFTAASFVLADDWLPQTGVPYTSGL 555
           +IN TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 545 VINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of Sed0022840 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 654.4 bits (1687), Expect = 7.9e-188
Identity = 331/547 (60.51%), Postives = 420/547 (76.78%), Query Frame = 0

Query: 24  ATQTGSVCRSTLDPAFCKTALPNQT-GNVYAYGRSSVRKSLSSSQKFLRTVEKQL-RGRA 83
           +  + ++C++T DP FCK+  P  + G+V  YGR S+RKSL+ S+KF RT+++ L R  A
Sbjct: 28  SNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQSRKFTRTIDRYLKRNNA 87

Query: 84  WLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSA--LPEMKAEEVASLLSALLTNQQ 143
            L+  AV AL+DC+ LA L  DYL  +  TV+ T+S+  L   KA+E+ +LLSA LTN+Q
Sbjct: 88  LLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSKADEIQTLLSAALTNEQ 147

Query: 144 TCMDGLK-ATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWVPKKKK----------RS 203
           TC+DG+  A + SW+++NG++LPL  DTKL+S+SLALFTKGWVPKKKK          ++
Sbjct: 148 TCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVPKKKKQVASYSWAHPKN 207

Query: 204 TWKGAGKQGGFGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVRDIVVVSQD 263
           T         F NG + +KM+   +A+YE+ +RR L    D+D   + + V DIV V+Q+
Sbjct: 208 THSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLA---DDDNDVNTVLVSDIVTVNQN 267

Query: 264 GSGNFTTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVI 323
           G+GNFTTI EA+ +APN +  T GYF+I+V++GVYEE V IAKNKRYLMMIGDGIN+TV+
Sbjct: 268 GTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDGINRTVV 327

Query: 324 TGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLSTFYLCSF 383
           TGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG EKHQAVA+RS ADLS FY CSF
Sbjct: 328 TGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSF 387

Query: 384 EAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNAITAQGRT 443
           EAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN++PR PM NQFNAITAQGRT
Sbjct: 388 EAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRT 447

Query: 444 DPNQNTGTSIQNCRITPADDLANAT-AVKTFLGRPWKEFSRTVYLQNFMDGFINPAGWRA 503
           DPNQNTG SI NC I PADDL ++   VKT+LGRPWKE+SRTV++Q+++D  + P GWR 
Sbjct: 448 DPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWRE 507

Query: 504 WDGEFALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLADDWLPQTG 555
           W+G+FAL+T YYAE+ N G GS+T++RV W G+H+IN TDA NFT  +F+L D W+ Q+G
Sbjct: 508 WNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDGWMVQSG 567

BLAST of Sed0022840 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 633.6 bits (1633), Expect = 1.5e-181
Identity = 315/537 (58.66%), Postives = 404/537 (75.23%), Query Frame = 0

Query: 24  ATQTGSVCRSTLDPAFCKTALPNQTGNVYAYGRSSVRKSLSSSQKFLRTVEKQLRGRAWL 83
           AT   ++CR   DP++C++ LPNQ G++Y+YGR S+R+SLS +++F+  ++ +L  +  +
Sbjct: 32  ATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKV 91

Query: 84  TV-GAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPEMKAEEVASLLSALLTNQQTCM 143
                V ALEDC+ LA L +DYL ++  T D T + L   +AE+V + LSA +TN+QTC+
Sbjct: 92  AAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKT-LSLSRAEDVHTFLSAAITNEQTCL 151

Query: 144 DGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWVPKKKK-RSTWKGAG---KQGG 203
           +GLK+TA     +NGLS  L  DTKLY +SLALF+KGWVP++++ R  W+      K  G
Sbjct: 152 EGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFG 211

Query: 204 FGNGRMSVKMSSRAKAIYEAAARRNLQQSNDEDGGDDQISVRDIVVVSQDGSGNFTTINE 263
           F NG++ +KM+ RA+A+Y    RR L QS+      D + V DIV V Q+G+GNFTTIN 
Sbjct: 212 FRNGKLPLKMTERARAVYNTVTRRKLLQSD-----ADAVQVSDIVTVIQNGTGNFTTINA 271

Query: 264 AIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVITGNRSVVDGW 323
           AIAAAPN +  + GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTVITGNRSVVDGW
Sbjct: 272 AIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGW 331

Query: 324 TTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLSTFYLCSFEAYQDTLYTH 383
           TTFNSATF + GP F+ VN+T RNTAG  K QAVA+RSG DLS FY CSFEAYQDTLYTH
Sbjct: 332 TTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTH 391

Query: 384 SLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNAITAQGRTDPNQNTGTSI 443
           SLRQFYR+CD+YGTVDFIFGNAAVV QNCN++PR P   Q N +TAQGRTDPNQNTGT+I
Sbjct: 392 SLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAI 451

Query: 444 QNCRITPADDLANAT-AVKTFLGRPWKEFSRTVYLQNFMDGFINPAGWRAWDGEFALNTS 503
             C I PADDLA +   VKT+LGRPWKE+SRTV +Q ++DGF+ P+GW AW G+FAL+T 
Sbjct: 452 HGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTL 511

Query: 504 YYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLADDWLPQTGVPYTSGL 555
           YYAE+ N GPGS+T+ RV W G+H+IN TDA NFT  +F++ + W+ QTGVP+  GL
Sbjct: 512 YYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of Sed0022840 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 475.3 bits (1222), Expect = 6.6e-134
Identity = 260/541 (48.06%), Postives = 352/541 (65.06%), Query Frame = 0

Query: 31  CRSTLDPAFCKT---ALPNQTGNVYAYGRSSVRKSLSSSQKFLRTVEKQLR----GRAWL 90
           C+ST  P  C+T   A+ +   + Y YG+ ++++ L  +++  + + +  +         
Sbjct: 88  CKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEADPGTS 147

Query: 91  TVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPEMKAEEVASLLSALLTNQQTCMD 150
           TV  V A+ DC  LA L+++YL   +T   + +  +     + V SLL  ++TNQQTC+D
Sbjct: 148 TVEEVSAVADCGELAELSVEYL-ETVTEELKAAELMTAALVDRVTSLLGGVVTNQQTCLD 207

Query: 151 GLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWVPKKKKRSTWKGA-GKQGGFGNG 210
           GL      ++    +  PL   T+LYS+SL L +   + +  KR  +KG+ GK  G GN 
Sbjct: 208 GLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKR--YKGSKGKIFGGGNK 267

Query: 211 RMSVKMSSRAKAIYEAA--------ARRNLQQSNDEDGGDDQISVRDIVVVSQDGSGNFT 270
            +   + +  K + +          A RNL +  +  GG   I VR+ V V    + NF 
Sbjct: 268 PVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGG--SILVREAVTVGPYETDNFP 327

Query: 271 TINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVITGNRSV 330
           TI EA+AAAPN++ P +GYF+I+  AG+YEEYV I+  KR +M+IGDGIN+T+I+GN S 
Sbjct: 328 TITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNHSF 387

Query: 331 VDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLSTFYLCSFEAYQDT 390
           +DGWTT+NS+TFAVVG  FVAV++TFRNTAG EKHQAVAVR+ AD STFY CSFE YQDT
Sbjct: 388 IDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQDT 447

Query: 391 LYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNAITAQGRTDPNQNT 450
           LY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNI+ R PMANQ NA+TA GRTDPNQ T
Sbjct: 448 LYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQKT 507

Query: 451 GTSIQNCRITPADDL-ANATAVKTFLGRPWKEFSRTVYLQNFMDGFINPAGWRAWDGEFA 510
           G SI NC I  A DL A+  +  TFLGRPWK +SRTVY+Q+++   + P GW  W+G   
Sbjct: 508 GISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNGTTG 567

Query: 511 LNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLADDWLPQTGVPYTSG 555
           L+T  Y E+ NFGPG++TS+RV W+G+ L+N   A NFT  +F L D WLPQT +P+  G
Sbjct: 568 LDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDIPFYGG 620

BLAST of Sed0022840 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 473.8 bits (1218), Expect = 1.9e-133
Identity = 256/542 (47.23%), Postives = 346/542 (63.84%), Query Frame = 0

Query: 31  CRSTLDPAFCKT---ALPNQTGNVYAYGRSSVRKSLSSSQKFLRTVEKQLR------GRA 90
           C+ST  P  C+T   A+ +   + Y YG+ ++++ L  + +  + +    R      G A
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 91  WLTVGAVRALEDCQLLAGLNIDYLAAAMTTVDRTSSALPEMKAEEVASLLSALLTNQQTC 150
             T   + A+ DC  L+ L+++YL   +TT  +T+  +     E V SLLS ++TNQQTC
Sbjct: 143 --TAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSLLSGVVTNQQTC 202

Query: 151 MDGLKATAGSWSLKNGLSLPLATDTKLYSMSLALFTKGWVPKKKKRSTWKGAGKQGGFGN 210
           +DGL      ++    +  P+   T+LYS+SL L +       K+    K  GK  G GN
Sbjct: 203 LDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASK--GKILGGGN 262

Query: 211 GRMSVKMSSRAKAIYEAA--------ARRNLQQSNDEDGGDDQISVRDIVVVSQDGSGNF 270
                 + +  K + +            RNL +  +  GG   I V   V+V    S NF
Sbjct: 263 STYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGG--SILVSKAVIVGPFKSDNF 322

Query: 271 TTINEAIAAAPNNSAPTEGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVITGNRS 330
           TTI +AIAAAPNN+ P +GYF+I+   GVYEEY+ +  NK+ LM++GDGIN+T+ITGN +
Sbjct: 323 TTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHN 382

Query: 331 VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAEKHQAVAVRSGADLSTFYLCSFEAYQD 390
           VVDGWTT+N ++FAVVG  F+AV++TFRNTAG EKHQAVA+R+ A+ S+FY CSFE YQD
Sbjct: 383 VVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQD 442

Query: 391 TLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIHPRLPMANQFNAITAQGRTDPNQN 450
           TLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNI+ R PMA Q NAITA GR DPNQN
Sbjct: 443 TLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQN 502

Query: 451 TGTSIQNCRITPADDL-ANATAVKTFLGRPWKEFSRTVYLQNFMDGFINPAGWRAWDGEF 510
           TG SI NC I  A DL A   +  TFLGRPWK +SRTV++Q+++   + P GW  W+G  
Sbjct: 503 TGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTI 562

Query: 511 ALNTSYYAEFGNFGPGSNTSERVNWTGFHLINDTDAGNFTAASFVLADDWLPQTGVPYTS 555
            L+T YY E+ NFGPG+NT++RV W G++L+N  +A NFT  +F + D WLPQT +P+  
Sbjct: 563 GLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIPFYG 615

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6593215.12.8e-26784.88putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrospe... [more]
KAG7030208.11.4e-26684.82putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrospe... [more]
XP_022959747.13.1e-26684.41probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata][more]
XP_038896831.16.8e-26683.99probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida][more]
XP_023513640.12.6e-26584.41probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SRX41.0e-19560.03Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
Q8RXK71.1e-18660.51Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
O222562.0e-18058.66Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Q9FF779.3e-13348.06Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Q94CB12.7e-13247.23Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1H5E41.5e-26684.41Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460725 PE=3 SV=1[more]
A0A5D3D3H41.3e-26583.30Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00132... [more]
A0A1S3BSI21.3e-26583.30Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1[more]
A0A6J1KSC06.9e-26484.44Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111497795 PE=3 SV=1[more]
A0A6J1JVJ51.5e-26383.12Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G02810.17.2e-19760.03Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.17.9e-18860.51Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.11.5e-18158.66Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.16.6e-13448.06Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.21.9e-13347.23Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 22..175
e-value: 4.2E-17
score: 72.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 29..175
e-value: 8.4E-14
score: 52.1
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 243..538
e-value: 1.5E-134
score: 447.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 237..554
e-value: 1.8E-118
score: 397.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 24..178
e-value: 5.3E-17
score: 64.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 29..178
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 17..554
NoneNo IPR availablePANTHERPTHR31707:SF294PECTINESTERASE/PECTINESTERASE INHIBITOR 7-RELATEDcoord: 17..554
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 29..180
e-value: 2.99398E-22
score: 91.3457
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 389..398
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 241..553

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0022840.1Sed0022840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity