Sed0021168 (gene) Chayote v1

BLAST of Sed0021168 vs. NCBI nr
Match: XP_038893074.1 (4-coumarate--CoA ligase-like 1 [Benincasa hispida])

HSP 1 Score: 991.9 bits (2563), Expect = 2.2e-285
Identity = 501/549 (91.26%), Postives = 528/549 (96.17%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           MA C RD V+D EHIFRSQLP VQVPDDITLPEFVLQNAESYA+NVAFVEAVSGKAYTY 
Sbjct: 1   MATCNRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           +VVRDT+RF+KAL SLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVVRDTNRFSKALWSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKE 180
           +KQVE A AKLV+T+SANFEKVKELKLPVI+LGEE IEG+MNWHKL+EAA RAGNN  KE
Sbjct: 121 KKQVEVAEAKLVITNSANFEKVKELKLPVILLGEELIEGSMNWHKLLEAADRAGNNCVKE 180

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHI 240
           DIKQ DLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVPQE+EGKVTTLGLIPFFHI
Sbjct: 181 DIKQNDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNP+V+EFD
Sbjct: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300

Query: 301 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 360
           LSGLKLQA+MTAAAPLAPELQSAFE+KFPGVDVQEAYGLTEHSCITLNYGSIGKENL  K
Sbjct: 301 LSGLKLQAIMTAAAPLAPELQSAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLAAK 360

Query: 361 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           KNTVGRILPNLEVKFIDPDSG+SLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420

Query: 421 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIG+IDDD DVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 481 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 540
           EIPAASVV+A N+KETE+EIIK+VASNVA YKKVRLVHFVD IPKSPSGKVMRRL+KEKM
Sbjct: 481 EIPAASVVMAPNSKETEEEIIKHVASNVANYKKVRLVHFVDAIPKSPSGKVMRRLVKEKM 540

Query: 541 IKKIRAESA 550
           I+KIRAESA
Sbjct: 541 IEKIRAESA 549

BLAST of Sed0021168 vs. NCBI nr
Match: XP_022945246.1 (4-coumarate--CoA ligase-like 1 [Cucurbita moschata])

HSP 1 Score: 991.1 bits (2561), Expect = 3.8e-285
Identity = 502/549 (91.44%), Postives = 529/549 (96.36%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           MA CIRD V+D EHIFRSQLP VQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTY 
Sbjct: 57  MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYR 116

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           +VVRDTSRFAKALSSLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKE 180
           +KQVE A+AKLVVT+S++FEKVKELKLPVIV+GEE IEG+MNWHKL+EAA RAGNN  KE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNP+V EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 360
           LSGLKLQA+MTAAAPLAPELQ++FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK+NL  K
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           KNTVGRILPNLEVKFIDPDSG+SLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIG+IDDD DVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 540
           EIPAAS+V+A NAKETEDEIIKYVASNVA YKKVRLVHFV+ IPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IKKIRAESA 550
           I+KIRAE+A
Sbjct: 597 IEKIRAEAA 605

BLAST of Sed0021168 vs. NCBI nr
Match: XP_023541569.1 (4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 989.9 bits (2558), Expect = 8.5e-285
Identity = 501/549 (91.26%), Postives = 529/549 (96.36%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           MA CIRD+V+D EHIFRSQLP VQVPDDITLPEFVLQNAESYADNVAFVEAVSG AYTY 
Sbjct: 57  MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGNAYTYR 116

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           +VVRDTSRFAKALSSLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKE 180
           +KQVE A+AKLVVT+S++FEKVKELKLPVIV+GEE IEG+MNWHKL+EAA RAGNN  KE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNP+V EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 360
           LSGLKLQA+MTAAAPLAPELQ++FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK+NL  K
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           KNTVGRILPNLEVKFIDPDSG+SLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIG+IDDD DVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 540
           EIPAAS+V+A NAKETEDEIIKYVASNVA YKKVRLVHFV+ IPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IKKIRAESA 550
           I+KIRAE+A
Sbjct: 597 IEKIRAETA 605

BLAST of Sed0021168 vs. NCBI nr
Match: KAG7012722.1 (4-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 988.8 bits (2555), Expect = 1.9e-284
Identity = 500/549 (91.07%), Postives = 529/549 (96.36%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           MA CIRD+V+D EHIFRSQLP VQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTY 
Sbjct: 57  MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYR 116

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           +VVRDTSRFAKALSSLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKE 180
           +KQVE A+AKLVVT+S++FEKVKELKLPVIV+GEE IEG+MNWH+L+EAA RAGNN  KE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNP+V EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 360
           LSGLKLQA+MTAAAPLAPELQ++FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK+NL  K
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           KNTVGRILPNLEVKFIDPDSG+SLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIG+IDDD DVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 540
           EIPAAS+V+  NAKETEDEIIKYVASNVA YKKVRLVHFV+ IPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IKKIRAESA 550
           I+KIRAE+A
Sbjct: 597 IEKIRAETA 605

BLAST of Sed0021168 vs. NCBI nr
Match: XP_022966799.1 (4-coumarate--CoA ligase-like 1 [Cucurbita maxima])

HSP 1 Score: 985.7 bits (2547), Expect = 1.6e-283
Identity = 497/549 (90.53%), Postives = 527/549 (95.99%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           MA CIRD+ +D EHIFRSQLP VQVPDDITLPEFVLQNAE YADNVAFVEAVSGKAYTY 
Sbjct: 57  MATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYR 116

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           +VVRDTSRFAKALSSLRL+KGQ+VIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKE 180
           +KQVE A+AKLVVT+S++FEKVKELKLPVIV+GEE IEG+MNWHKL+EAA RAGNN  KE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNP+V EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 360
           LS LKLQA+MTAAAPLAPELQ++FEKKFPGVDVQEAYGLTEHSCITLNYG+IGK+NL  K
Sbjct: 357 LSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAK 416

Query: 361 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           KNTVGRILPNLEVKFIDPDSG+SLPKNTPGEICVRSQCVMQGYY NEEETSRTIDNKGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWM 476

Query: 421 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIG+IDDD DVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 540
           EIPAAS+V+A NAKETEDEIIKYVASNVA YKKVRLVHFV+ IPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IKKIRAESA 550
           I+KIRAE+A
Sbjct: 597 IEKIRAETA 605

BLAST of Sed0021168 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 2.4e-226
Identity = 390/532 (73.31%), Postives = 456/532 (85.71%), Query Frame = 0

Query: 10  QDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYHDVVRDTSRF 69
           +DNE+IFRS  PSV +PD +TLPEFVLQ  E Y +NVAFVEAV+GKA TY DVVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 70  AKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIEKQVETANA 129
           AKAL+SL LRKGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEI+KQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 130 KLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKEDIKQTDLCA 189
           + ++TD+ N+EKVK L LPVIVLGEE+IEGA+NW  L+EA  + G+  + E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 190 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHIYGITGICCA 249
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  E+ G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 250 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFDLSGLKLQAV 309
           T++NKGKVV M R+DLR F+NALI HE++FAPIVPPIIL LVKNP+VDEFDLS LKLQ+V
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 310 MTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGKKNTVGRILP 369
           MTAAAPLAPEL +AFE KFP V VQEAYGLTEHSCITL +G   K     K+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 370 NLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGFID 429
           NLEVKFIDPD+G+SLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIG+ID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 430 DDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVL 489
           DD D+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 490 ASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKMI 542
              A E E++I+ +VA+NVA YKKVR VHFVD IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of Sed0021168 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 699.1 bits (1803), Expect = 3.9e-200
Identity = 345/539 (64.01%), Postives = 440/539 (81.63%), Query Frame = 0

Query: 10  QDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVS-GKAYTYHDVVRDTSR 69
           ++ EH+FRS+ P V VPD +T+PEFVL  AE+YAD VA VEA + G++YTY +V RDT+R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 70  FAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIEKQVETAN 129
           FA+AL S+ +RKG VV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EI+KQVE + 
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 130 AKLVVTDSANFEKVKELKLPVIVLGE-EQIEGAMNWHKLIEAAGRAGNNLEKED-IKQTD 189
           AKLVV +   F+KVK+  +PVI +G+ E++ GA++W  L+ AA R G  +   D  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 190 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHIYGITGI 249
           LCALP+SSGTTGVSKGVML+HRNLV+NLCS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 250 CCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFDLSGLKL 309
           CCATLR+KG VVVM RFDLRTF+ AL+ H + FAP+VPP++LA+VK+P+ DEFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 310 QAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGKKNTVGR 369
           ++VMTAAAPLAP+L +AF++KFPGV V+EAYGLTEHSCITL + +        KK++VG 
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 370 ILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 429
           ILPNLEVKF+DPD+G+SLP NTPGE+CVRSQ VMQGYYK +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 430 FIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 489
           +ID D DVFIVDRIKELIKYKGFQVAPAELEA+LL+HPS+EDAAV  +PDEEAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 490 VVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKMIKKIR 546
           VV    A+E E+EI+ YVA  VA+YK+VR++H VD IPKS SGK++RR ++++ IK+++
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

BLAST of Sed0021168 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.3e-115
Identity = 214/547 (39.12%), Postives = 327/547 (59.78%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           MA  +    Q  + IFRS+LP + +P ++ L  +  +N   ++     +   + + +TY 
Sbjct: 1   MAAAVAIEEQKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYA 60

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           DV   + R    LS L +++G  ++++LPN  E+    LG    G + +  NP    +E+
Sbjct: 61  DVELISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEV 120

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLP-----VIVLGEEQIEGAMNWHKLIEAAGRAGN 180
            KQ + +NAKL++T     +KVK+         + +      + A N     E  G   N
Sbjct: 121 IKQAKASNAKLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADEN 180

Query: 181 NLEKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEL--EGKVTTLG 240
            + K +I    + ALP+SSGTTG+ KGVMLTH+ LV ++   + G    L        L 
Sbjct: 181 EMPKVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLC 240

Query: 241 LIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKN 300
           ++P FHIY +  +    LR    +++M +F++  F+  +  +++T  P VPPI+LA+ K+
Sbjct: 241 VLPLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKS 300

Query: 301 PMVDEFDLSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIG 360
            +VD +DLS ++   VM+ AAPL  EL+ A   KFP   + + YG+TE   +     +  
Sbjct: 301 TVVDNYDLSSVR--TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFA 360

Query: 361 KENLPGKKNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRT 420
           KE    K    G ++ N E+K +DP++G SLP+N PGEIC+R   +M+GY  + E T+RT
Sbjct: 361 KEPFDIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATART 420

Query: 421 IDNKGWMHTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVP 480
           ID +GW+HTGDIG+IDDDD++FIVDR+KELIKYKGFQVAPAELEA+LLTHP I DAAVVP
Sbjct: 421 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVP 480

Query: 481 LPDEEAGEIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMR 540
           + DE AGE+P A VV ++    TEDEI ++++  V  YK++  V FV+ IPK+PSGK++R
Sbjct: 481 MKDEAAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILR 540

BLAST of Sed0021168 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 5.1e-115
Identity = 207/536 (38.62%), Postives = 332/536 (61.94%), Query Frame = 0

Query: 10  QDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYHDVVRDTSRF 69
           Q  + IFRS+LP + +P  + L  +  +N   +      ++  + + YTY +V   + + 
Sbjct: 9   QSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKV 68

Query: 70  AKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIEKQVETANA 129
           A  L+ L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + ++A
Sbjct: 69  AVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSA 128

Query: 130 KLVVTDSANFEKVKELKLP---VIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKEDIKQTD 189
           K+V+T +    KVK+  +     ++  +   EG +++ +LI++     + +    I+  D
Sbjct: 129 KIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQS---DEHEIPDVKIQPDD 188

Query: 190 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEL--EGKVTTLGLIPFFHIYGIT 249
           + ALP+SSGTTG+ KGVMLTH+ LV ++   + G    L        + ++P FHIY + 
Sbjct: 189 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLN 248

Query: 250 GICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFDLSGL 309
            +    LR    +++M +FD+  F+  +  H++T  P VPPI+LA+ K+P+VD +DLS +
Sbjct: 249 SVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSV 308

Query: 310 KLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGKKNTV 369
           +   VM+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    K    
Sbjct: 309 R--TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 368

Query: 370 GRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGD 429
           G ++ N E+K +DPD+G SLP+N PGEIC+R   +M+GY  + E T+RTI+ +GW+HTGD
Sbjct: 369 GTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGD 428

Query: 430 IGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPA 489
           IGFIDDDD++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ DE+AGE+P 
Sbjct: 429 IGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPV 488

Query: 490 ASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 541
           A VV ++ +  TEDE+  +++  V  YK+++ V FV+ +PKSPSGK++R+ ++ ++
Sbjct: 489 AFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

BLAST of Sed0021168 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.1e-114
Identity = 215/545 (39.45%), Postives = 335/545 (61.47%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           M +C+       + IFRS+LP + +P  + L  +  +N     D    +   +G+ +TY 
Sbjct: 1   MGDCV---APKEDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYS 60

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
            V   + + A  L+ L +++G  ++++LPN  EY    LG    G + +  NP    +E+
Sbjct: 61  QVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEV 120

Query: 121 EKQVETANAKLVVTDSANFEKVKEL---KLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNL 180
            KQ++ + AKL++T +   +KVK+    K   I+  ++  +  +++ KL+EA     + +
Sbjct: 121 IKQLKASQAKLIITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEA---DESEM 180

Query: 181 EKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEL--EGKVTTLGLI 240
            +  I   D+ ALP+SSGTTG+ KGVMLTH+ LV ++   + G    L    +   + ++
Sbjct: 181 PEVVINSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICIL 240

Query: 241 PFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPM 300
           P FHIY +  + C  LR    +++M +FD+  F+  +  +++T  P VPPI+LA+ K+P+
Sbjct: 241 PLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPV 300

Query: 301 VDEFDLSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKE 360
           VD++DLS ++   VM+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE
Sbjct: 301 VDKYDLSSVR--TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKE 360

Query: 361 NLPGKKNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTID 420
               K    G ++ N E+K +DP++  SLP+N  GEIC+R   +M+GY  + E T  TID
Sbjct: 361 PYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTID 420

Query: 421 NKGWMHTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLP 480
            +GW+HTGDIGFIDDDD++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ 
Sbjct: 421 EEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMI 480

Query: 481 DEEAGEIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRL 540
           DE+AGE+P A VV  +    TE+EI ++V+  V  YK++  V FVD IPKSPSGK++R+ 
Sbjct: 481 DEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKD 537

BLAST of Sed0021168 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 991.1 bits (2561), Expect = 1.8e-285
Identity = 502/549 (91.44%), Postives = 529/549 (96.36%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           MA CIRD V+D EHIFRSQLP VQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTY 
Sbjct: 57  MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYR 116

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           +VVRDTSRFAKALSSLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKE 180
           +KQVE A+AKLVVT+S++FEKVKELKLPVIV+GEE IEG+MNWHKL+EAA RAGNN  KE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNP+V EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 360
           LSGLKLQA+MTAAAPLAPELQ++FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK+NL  K
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           KNTVGRILPNLEVKFIDPDSG+SLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIG+IDDD DVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 540
           EIPAAS+V+A NAKETEDEIIKYVASNVA YKKVRLVHFV+ IPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IKKIRAESA 550
           I+KIRAE+A
Sbjct: 597 IEKIRAEAA 605

BLAST of Sed0021168 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 7.7e-284
Identity = 497/549 (90.53%), Postives = 527/549 (95.99%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           MA CIRD+ +D EHIFRSQLP VQVPDDITLPEFVLQNAE YADNVAFVEAVSGKAYTY 
Sbjct: 57  MATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYR 116

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           +VVRDTSRFAKALSSLRL+KGQ+VIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKE 180
           +KQVE A+AKLVVT+S++FEKVKELKLPVIV+GEE IEG+MNWHKL+EAA RAGNN  KE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNP+V EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 360
           LS LKLQA+MTAAAPLAPELQ++FEKKFPGVDVQEAYGLTEHSCITLNYG+IGK+NL  K
Sbjct: 357 LSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAK 416

Query: 361 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           KNTVGRILPNLEVKFIDPDSG+SLPKNTPGEICVRSQCVMQGYY NEEETSRTIDNKGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWM 476

Query: 421 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIG+IDDD DVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 540
           EIPAAS+V+A NAKETEDEIIKYVASNVA YKKVRLVHFV+ IPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IKKIRAESA 550
           I+KIRAE+A
Sbjct: 597 IEKIRAETA 605

BLAST of Sed0021168 vs. ExPASy TrEMBL
Match: A0A6J1DG05 (4-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 1.0e-280
Identity = 495/549 (90.16%), Postives = 524/549 (95.45%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           M   IRD V+D EHIFRSQLP VQVPDDITLPEFVLQNAESYA+NVAFVEAVSGKAYTY 
Sbjct: 60  MGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 119

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           +VVRDT RFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 120 EVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 179

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKE 180
           +KQVE A+AKLVVT+S NFEKVKELKLPVIVLGEE IEG+MNWHKL+EAA RAGNN  KE
Sbjct: 180 KKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNHVKE 239

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHI 240
           DIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E EGKVTTLGLIPFFHI
Sbjct: 240 DIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIPFFHI 299

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNP+V+EFD
Sbjct: 300 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 359

Query: 301 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 360
           LS LKLQA+MTAAAPLAPE+Q+AFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENL  K
Sbjct: 360 LSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 419

Query: 361 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           KNTVGRILPNLEVKFIDP+SG+SLPKNTPGEICVRSQCVMQGYY NEEETSRTIDN+GW+
Sbjct: 420 KNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNRGWL 479

Query: 421 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIG+IDD+ DVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 480 HTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 539

Query: 481 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 540
           EIPAASVV+A N+KETE+EII+YVASNVA YKKVR+VHFVD IPKSPSGKVMRRLIKEKM
Sbjct: 540 EIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLIKEKM 599

Query: 541 IKKIRAESA 550
           I KIRA++A
Sbjct: 600 IDKIRAKTA 608

BLAST of Sed0021168 vs. ExPASy TrEMBL
Match: A0A6J1DEE1 (4-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 1.0e-280
Identity = 495/549 (90.16%), Postives = 524/549 (95.45%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           M   IRD V+D EHIFRSQLP VQVPDDITLPEFVLQNAESYA+NVAFVEAVSGKAYTY 
Sbjct: 9   MGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 68

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           +VVRDT RFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 69  EVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 128

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKE 180
           +KQVE A+AKLVVT+S NFEKVKELKLPVIVLGEE IEG+MNWHKL+EAA RAGNN  KE
Sbjct: 129 KKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNHVKE 188

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHI 240
           DIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E EGKVTTLGLIPFFHI
Sbjct: 189 DIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIPFFHI 248

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNP+V+EFD
Sbjct: 249 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 308

Query: 301 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 360
           LS LKLQA+MTAAAPLAPE+Q+AFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENL  K
Sbjct: 309 LSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 368

Query: 361 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           KNTVGRILPNLEVKFIDP+SG+SLPKNTPGEICVRSQCVMQGYY NEEETSRTIDN+GW+
Sbjct: 369 KNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNRGWL 428

Query: 421 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIG+IDD+ DVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 429 HTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 488

Query: 481 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 540
           EIPAASVV+A N+KETE+EII+YVASNVA YKKVR+VHFVD IPKSPSGKVMRRLIKEKM
Sbjct: 489 EIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLIKEKM 548

Query: 541 IKKIRAESA 550
           I KIRA++A
Sbjct: 549 IDKIRAKTA 557

BLAST of Sed0021168 vs. ExPASy TrEMBL
Match: A0A0A0KRZ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.1e-277
Identity = 486/549 (88.52%), Postives = 523/549 (95.26%), Query Frame = 0

Query: 1   MANCIRDAVQDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYH 60
           MA C +D+VQD EHIF SQLP VQVP DITLPEFVLQNAESYA+NVAFVEA+SGKAYTY 
Sbjct: 1   MATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR 60

Query: 61  DVVRDTSRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           +V+RDT+RF+KAL SLRL+KG VVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 121 EKQVETANAKLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKE 180
           +KQVE A AKLVVT++ANFEKV+ELKLPVI+L EE +EGAMNWHKL+EAA RAGNN  KE
Sbjct: 121 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 180

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHI 240
           +IKQTDLCALPFSSGTTGVSKGVMLTHRNLVAN+CSTLSGVPQE+EGKVTTLGLIPFFHI
Sbjct: 181 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 300
           YGITGICC+TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNP+V+EFD
Sbjct: 241 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 300

Query: 301 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 360
           LS LKLQA+MTAAAPLAPELQ+AFE+KFPGVDVQEAYGLTEH CITLNYGSIGKENL  K
Sbjct: 301 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAK 360

Query: 361 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           KNTVGRILPNLEVKFIDPD+G+SLPKNTPGEICVRSQCVMQGYYKNEEETSRTID KGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM 420

Query: 421 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIG+IDDD +VFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 481 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 540
           EIPAASVV+A N+KETE++IIK+VASNVA YKKVRLVHFVD IPKSPSGKVMRRLIKEKM
Sbjct: 481 EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540

Query: 541 IKKIRAESA 550
           I+KIRA+ +
Sbjct: 541 IEKIRADES 549

BLAST of Sed0021168 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 786.2 bits (2029), Expect = 1.7e-227
Identity = 390/532 (73.31%), Postives = 456/532 (85.71%), Query Frame = 0

Query: 10  QDNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYHDVVRDTSRF 69
           +DNE+IFRS  PSV +PD +TLPEFVLQ  E Y +NVAFVEAV+GKA TY DVVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 70  AKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIEKQVETANA 129
           AKAL+SL LRKGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEI+KQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 130 KLVVTDSANFEKVKELKLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKEDIKQTDLCA 189
           + ++TD+ N+EKVK L LPVIVLGEE+IEGA+NW  L+EA  + G+  + E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 190 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELEGKVTTLGLIPFFHIYGITGICCA 249
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  E+ G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 250 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFDLSGLKLQAV 309
           T++NKGKVV M R+DLR F+NALI HE++FAPIVPPIIL LVKNP+VDEFDLS LKLQ+V
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 310 MTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGKKNTVGRILP 369
           MTAAAPLAPEL +AFE KFP V VQEAYGLTEHSCITL +G   K     K+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 370 NLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGFID 429
           NLEVKFIDPD+G+SLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIG+ID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 430 DDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVL 489
           DD D+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 490 ASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKMI 542
              A E E++I+ +VA+NVA YKKVR VHFVD IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of Sed0021168 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 403.3 bits (1035), Expect = 3.2e-112
Identity = 208/535 (38.88%), Postives = 329/535 (61.50%), Query Frame = 0

Query: 12  NEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYHDVVRDTSRFAK 71
           N+ IFRS+LP + +P+ + L +++ +N   +A     +   +G+ YTY DV   + + A 
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAA 78

Query: 72  ALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIEKQVETANAKL 131
            L +L +++  VV+++LPN  E  +  L     G + +  NP    +EI KQ + + AKL
Sbjct: 79  GLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKL 138

Query: 132 VVTDSANFEKVKELK---LPVIVLGEEQI-EGAMNWHKLIEAAGRAGNNLEKEDIKQTDL 191
           +VT S   +K+K L+   + ++    + I E  + + +L ++     +++  E I   D+
Sbjct: 139 IVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSI-PEKISPEDV 198

Query: 192 CALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEL--EGKVTTLGLIPFFHIYGITG 251
            ALPFSSGTTG+ KGVMLTH+ LV ++   + G    L        L ++P FHIY +  
Sbjct: 199 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 258

Query: 252 ICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFDLSGLK 311
           I   +LR    +++M +F++   +  +   ++T A +VPPI+LA+ K+P  +++DLS ++
Sbjct: 259 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 318

Query: 312 LQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGKKNTVG 371
           +  V + AAPL  EL+ A   KFP   + + YG+TE   +        KE  P K    G
Sbjct: 319 M--VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACG 378

Query: 372 RILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDI 431
            ++ N E+K +DPD+G SLP+N PGEIC+R   +M+GY  +   T+ TID  GW+HTGD+
Sbjct: 379 TVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDV 438

Query: 432 GFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAA 491
           GFIDDDD++FIVDR+KELIKYKGFQVAPAELE++L+ HP I D AVV + +E+AGE+P A
Sbjct: 439 GFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVA 498

Query: 492 SVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 541
            VV + ++  +EDEI ++V+  V  YK++  V F D IPK+PSGK++R+ ++ ++
Sbjct: 499 FVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARL 550

BLAST of Sed0021168 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 400.2 bits (1027), Expect = 2.7e-111
Identity = 205/540 (37.96%), Postives = 326/540 (60.37%), Query Frame = 0

Query: 11  DNEHIFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYHDVVRDTSRFA 70
           +++ IFRS+LP + +P+ ++L +++ QN   +A     +   +G  YTY DV   + + A
Sbjct: 20  NSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 79

Query: 71  KALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIEKQVETANAK 130
                L + +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ + +N K
Sbjct: 80  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 139

Query: 131 LVVTDSANFEKVKELK-----LPVIVLGEEQI---EGAMNWHKLIEAAGRAGNNLEKEDI 190
           L++T++   +K+K L+     + V +   E +   EG + + +L ++   A   ++  +I
Sbjct: 140 LIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 199

Query: 191 KQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEL--EGKVTTLGLIPFFHI 250
              D+ ALP+SSGTTG+ KGVMLTH+ LV ++   + G    L        L ++P FHI
Sbjct: 200 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 259

Query: 251 YGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFD 310
           Y +  I    LR    +++M +F++   +  +   ++T AP+VPPI+LA+ K+   +++D
Sbjct: 260 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 319

Query: 311 LSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGK 370
           LS +++  V + AAPL  EL+ A   KFP   + + YG+TE   +        KE  P K
Sbjct: 320 LSSIRV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 379

Query: 371 KNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 430
               G ++ N E+K +DPD+G SL +N PGEIC+R   +M+GY  N   T+ TID  GW+
Sbjct: 380 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 439

Query: 431 HTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 490
           HTGDIG IDDDD++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I D AVV + +E AG
Sbjct: 440 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 499

Query: 491 EIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 541
           E+P A VV + +++ +ED++ ++V+  V  YK++  V F + IPK+PSGK++R+ ++ K+
Sbjct: 500 EVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of Sed0021168 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 389.4 bits (999), Expect = 4.7e-108
Identity = 208/530 (39.25%), Postives = 320/530 (60.38%), Query Frame = 0

Query: 15  IFRSQLPSVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYHDVVRDTSRFAKALS 74
           IFRS+LP + +P+ + L  +  +   S +D    +   +GK+YTY +      R A  L 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 75  SLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIEKQVETANAKLVVT 134
            L +RKG V++++L N AE+    +G    G V +  NP     E+ KQ++++ AKL++T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 135 DSANFEKVKEL--KLPVIVLGEEQIEGAMNWHKLIEAAGRAGNNLEKEDIKQTDLCALPF 194
            S   +K+K L   L +I   E   E  + +  LI          E  DI   D  ALPF
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI-TDDETNPFQETVDIGGDDAAALPF 213

Query: 195 SSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--LEGKVTTLGLIPFFHIYGITGICCAT 254
           SSGTTG+ KGV+LTH++L+ ++   + G      L+     L ++P FHIY +  +   +
Sbjct: 214 SSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNS 273

Query: 255 LRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPMVDEFDLSGLKLQAVM 314
           LR+   V++M +F++   ++ +  H +T A +VPP+++AL KNP V+ +DLS ++   V+
Sbjct: 274 LRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF--VL 333

Query: 315 TAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLPGKKNTVGRILPN 374
           + AAPL  ELQ +  ++ P   + + YG+TE   +        KE +P K  + G ++ N
Sbjct: 334 SGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRN 393

Query: 375 LEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGFIDD 434
            E+K +  ++  SL  N PGEIC+R Q +M+ Y  + E TS TID +GW+HTGDIG++D+
Sbjct: 394 AELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDE 453

Query: 435 DDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVLA 494
           DD++FIVDR+KE+IK+KGFQV PAELE++L+ H SI DAAVVP  DE AGE+P A VV +
Sbjct: 454 DDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRS 513

Query: 495 SNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRRLIKEKM 541
           +    TE+++ +YVA  V  YK++  V FV  IPKSPSGK++R+ +K K+
Sbjct: 514 NGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Sed0021168 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 380.6 bits (976), Expect = 2.2e-105
Identity = 198/546 (36.26%), Postives = 327/546 (59.89%), Query Frame = 0

Query: 12  NEHIFRSQLPSVQVPDDITLPEFVLQ----NAESYADNVAFVEAVSGKAYTYHDVVRDTS 71
           ++ IFRS+LP + +P+ + L ++V Q    + +  +     ++  +G+  TY DV  +  
Sbjct: 25  HDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNMR 84

Query: 72  RFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIEKQVETA 131
           R A  +  L +R G VV+++LPN  E+A+  L +   G V +  NP     EI KQ + +
Sbjct: 85  RIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKAS 144

Query: 132 NAKLVVTDSANFEKVKELKLPVIVL------GEEQI-----EGAMNWHKLIEAAGRAGNN 191
            AK+++T     +K+  LK   +++      G+  +     +G +++ +L +A       
Sbjct: 145 AAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQA---DETE 204

Query: 192 LEKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQELE--GKVTTLGL 251
           L K  I   D  A+P+SSGTTG+ KGVM+TH+ LV ++   + G    L        L  
Sbjct: 205 LLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCF 264

Query: 252 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNP 311
           +P FHIY +  +  + +R    ++++ RF+L   +  +  +++T  P+ PP++LA +K+P
Sbjct: 265 LPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSP 324

Query: 312 MVDEFDLSGLKLQAVMTAAAPLAPELQSAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 371
             + +DLS +++  +++ AA L  EL+ A   KFP     + YG+TE   +  +  +  K
Sbjct: 325 ETERYDLSSVRI--MLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVAKSL-AFAK 384

Query: 372 ENLPGKKNTVGRILPNLEVKFIDPDSGKSLPKNTPGEICVRSQCVMQGYYKNEEETSRTI 431
                K    G ++ N E+K +D ++G SLP+N  GEICVR   +M+GY  + E T+RTI
Sbjct: 385 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 444

Query: 432 DNKGWMHTGDIGFIDDDDDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 491
           D  GW+HTGDIGF+DDDD++FIVDR+KELIK+KG+QVAPAELEA+L++HPSI+DAAVV +
Sbjct: 445 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 504

Query: 492 PDEEAGEIPAASVVLASNAKETEDEIIKYVASNVATYKKVRLVHFVDIIPKSPSGKVMRR 541
            DE A E+P A V  +  ++ TED++  YV   V  YK++++V F+++IPK+ SGK++R+
Sbjct: 505 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 564