Sed0020417 (gene) Chayote v1

Overview
NameSed0020417
Typegene
OrganismSechium edule (Chayote v1)
DescriptionCytochrome P450
LocationLG05: 45252888 .. 45257407 (-)
RNA-Seq ExpressionSed0020417
SyntenySed0020417
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAACTTTGAAATGGACTTTCTCACTCTTCTCTTTCTCCTCCTTGTTGCTTTCTTCATCCTCCAACAGCTCAAAACCCGCCGGAAAAATCTGCCGCCGTCTCCGCCGTGTGTTCCGGTCATCGGCCACCTCCACCTCCTCAAACGCCCCATCCACCGGAATTTTCAAACCATCGCCGCCAAGTACGGCCCCATTTTCACCCTCCGACTCGGATCTCGCCTTGCCCTCGTTGTCTCTTCCCTCCAAATCGCCGAACAATGTTTCACTACAAACGACGTCGTCTTCGCCAACCGCCCCCGCCTGCTCATCAGCCGGTAATGTTACATAAATTCTAGTTTCTAATGTTTCAAACCATAAGACTGAATTCATATTTTAACCTTTAAATTTTGTAAATTTGATTTTCAGATTATGTTCCTATATTCAATTTTGTTGTGTATACATTCATCACGCATCTTCCTAATTATTGGAGCATCTTTTCAATTCCACTTGTTGCGTCTTCCAATTGGCAAGTGCCTTATGTATTTATTATTATAAATTTTGTCAATAAAAAAAAGAGTTTGAATTTAATTTTCAAAAATTATAATTTAGTCACGACTTGAAAATAGTTAGAATTTAATCTACATCATTTTAAAAATTAGAATTTTGATAAAATCCAAAAATTAGTCATGGTCCATTGTAATCATCATAAATTATGCACTTGAAAATTAACTTTTTGTTTTTGAATTTTTTATCCAATAAATAAGGTACTTAATTCACACGGTTAAAACATGTGAATAACTAATTTTTACGTATTCAATTTTTAAATAATATTGACTATTTTTAAGATTTTATCAAAGTATAGAGACTAAATTTTAATTACGAAGGTCAATGGCACAGTTGATTATCTTTTTTTGTGTTAATCTATTTCAACTCTATTGTCACATGGTGGAGCTCTGAAATTAGTATCCGGTTCTCATATTATTTGAGAGTAATTTTGTCCTCTATGTAGGGTTTGTTGTCCCCTTTCTCCACCTTATACTAAAAAAAAAGATTAAATTTTAATTTTGAAAATTCATACAGATTAATTCTAAGTTTTTTTTTTAAATGTGAATTAAATTTTAATTTTTATAATTTATCTTTTATTATTATTAAGTCAATTATTAATGATTTGATAGTTACCTGGGCTACAACTGCACCACCATCTCCACCGCCCCGTACGGCGACTACTGGCGGAACCTCCGCCGCCTCTCCGCCGTGGAGATCTTCTCGACGGCAAAACTAAACGCATCTCTGTGCATCCGCAAGGAAGAAATCCGGCGATTCCTCCTGAAACTCCACTCGGAATGGTTCGCCAAAGCGGAGCTGAAATCGATGTTTTCGGAACTGAGCTTCAACGTGGTGATGCGGGTGGTGGCCGGAAAACGGTACTACGGCGAGGAGGTTTCCGACGAGGCGGAGGCGAGGGAGTTCCGGGAGCTGATGGAGGAGATTTCGGCCCAGGGCGGGGCTTCGCATTGGGAGGATTTTATGCCGATCTTGAAATGGATCGGGCGCGGTGGATTCGAGAACAGTTTGAAGAAATCCGGGGAAAGGGCGGATCGGTTTATGCAGGCGTTGATTGAAGAACGCCGGAATTTGAAACTGCTCGGAACTGAAAAACAGAGGAATCTGCTTGATCGGCTTTTGGAGTTGCAGCTTTCTGAGCCGGAATATTACTCCGATCACACAATTAAAGGGATCGTTCTGGTTAGCAACCCAACTTTTTAATTCAATCTTTTTTTTTTTTTTTTTTTTTTAAGTTTATGGAACAAAATGTTATTTTAAATATGTTATTTTCCTCCTTTCTACTTTTTCTTGTCATATATTACTATATTTGACAAAGAAACATACCAAAGGGATCACAATATAAGAAAAGGTACTTTTGTCATCGAAAAAGAAAGTCCGACAACTAAGATAGAAATTGGAGGAAGGTGATATATATATATACGAAAAAGTATGGTACATAAATCAAGCACAACACACAAAAATTCTTAACGTGAAAATGAGTAAAAAATCACAAACCGAAGTTAAATCTCTACTATAATCGATAATGGTCAACAACAAGTTCTCTCTAGTTACAACCAGAGGTATACAACATATAACAACTCAATATAAACAATTTTGGCAACAAACACAAACCAAAAAATAAATAAAAAGATCTCACAAAATTGTGGAACCGAATCGTCACTGAGATCTGGATGTAGAAACCTCTAAAACCCGATCCCACCGTTCGGATAGAAGACCCTCGTCTAGAGAACACGCTGTCCAAATTTCAACTCAATCCAACGGTGAAATTGCTCGAAAACACCAAAATATTAAAGCTGGATGAAACCTCATTTTCTCTCTATTTTTTCTCTCTCTAAGTTATTGTTTGTCCTAATTTTCTAGGTTTGGATCGATCAAAGTGACAAAATTCTTTTGAAATACTTAGCTCTAATAAGCCTTATTTGATGAGTTTCACAAAGAATGAATAAAACCCAACAATTTTATTGATATTTTAGATATATTTTATTTTGGTTCGATTTCCAATAAAACTTTGCATATTTCAATTGTGTAGTAAGGGTTAAAATTTCATTTAAAAAATTGAACGAGAATGATATTTTGTTAATTTTTCCAACTCAGGGAGTGTCATCACATTTAAAACCATTTTATAAATTTTCTATGATTGTAATTTTGGACAGGTGTTGTTGCGTGCCGGAACAGATACATCATCTGTGACTTTGAATTGGGCAATGACTCAACTACTCAACAATCCGGAAATGTTGGCCAAAGCAAGAGCTGAGATAGACACCAAAATCGGCCAAGATCGCCCCATCAACGAAGGAGATCTCCCCAACCTCGACTACCTCCAAGCCATTATTTCCGAGACCTTCCGCCTTCACCCTGCCGCACCGATGCTCCTCACACACTACTCCTCTGACGATTGCGTCGTTGCAGGCTACGACATTCCTCGTGGGACAACGTTGTTGGTCAATGCCTGGGCCATTCACCGAGACCCGAAGTTGTGGGACGACCCGACGAGCTTCAACCCAGAGAGGTTTTTTGGGCCAGGAAATGAGTTGAACTCAAACAAAATAATTCCTTTTGGGATTGGAAGGAGGGCTTGTCCTGGTGCGAGCATGGCTCAACGTTTCATAGGATTGACTTTGGGGCTATTGATTCAATGCTATGAGTGGAAAAAGGATGGAGAAGAAAATGTGGACATGGCCGAAGGTGGAGGCATTACTATTCATAAAGTCAAGCCTTTGGAAACTATGTGCAAAGCTCGCCCAATTATGGTTAGAGTTCTATCCAATGGTTTGGACTAAATTTGAACAAGGTTTGGTTACTTAGACCTTTCCTCAATGGCTTAACTAAATAATGTAGTTACTACTAGATGGTTCTTATTAAATGAATGTTATAATCACTATGATATATATATATTTTAATAATAACTTCAATATCTAAAAATATCGAAGATTGAGAGCACATTTGAGGGTGAAGGTAGACTTACTACCTTTTGAAACATCTTCAAAATATCTACTAGATGTTTTTTTTTGGGTGGTATTTAGCAATAGTAAGTAATTTTTGAAACATCAAAAAGTTACTTTAAGTAATTTTCAATAAATAATCTAGATGTTGAGTTATTTTGTTTTTAAAGATTTCTTAAAAATAAAATTGAGAAATTGGAATTTCGCCCTAAAGATAAAGTATCAACTATATGCATGAAGCAATGATCATTTAAAAATGGTTGTCCATACTTATTCTGTGTCTCACTTCTTCTAATCAAATTTAATATATAGGAGTATGGATAATTGAAATGTACGTTTACGGATAATTTCAACTTAGATAAAAAAGAATGCAATTCTATTGATTCTAAATATTATTATTTTATGTCTACTTACAAAAAGCAAGGTTTTATTTCTAAAATCTTCACCTATATTGTTTTCATGCAAGCATGTGTTCAATTTCATTTTTAAAATTAAATCTTACAAATTATGCTAATTCAAATGATCAATTTAAGCTAAAGATAAAACTTAATAAAAGTACTAAATAATGTCAAATAAGTTGAGCCAAGTTGTTTAAGCATAAAGTGAGATTTTCTTCCTTCTTAAAATATTACATTTAATTAATTTTCCATGTAAGACAAACTTGAATAAAAAAAATAAAAAATAAAAAAAGACAAACTTGAATAAAAAAAAAATTGAACTTCATTGAAAAAGTTGTGAACTTTTGAAATAACCAACTTAAGTACAAATAACTAAAATTAAAACTAAACTGTACACCTTTTATCCAAAAAAAGAAAAGAAAAGAAAGAAAGAAAGAAAAACAGAACTTACAAAACTCAAACACACTAGAAACATTATCAGCAAACTTTAATACCAGGAAATAGAAATCAGTAATTCCCAGAAAACTTGAATTTACACAGCTGATTTCATCCTATGTAAAGAGACAATTTAAAACCAATAAGGCAATGCATATAAAATATAAAGGAAAAATTGGTCATAATCATAATCTTGAAC

mRNA sequence

GTGAACTTTGAAATGGACTTTCTCACTCTTCTCTTTCTCCTCCTTGTTGCTTTCTTCATCCTCCAACAGCTCAAAACCCGCCGGAAAAATCTGCCGCCGTCTCCGCCGTGTGTTCCGGTCATCGGCCACCTCCACCTCCTCAAACGCCCCATCCACCGGAATTTTCAAACCATCGCCGCCAAGTACGGCCCCATTTTCACCCTCCGACTCGGATCTCGCCTTGCCCTCGTTGTCTCTTCCCTCCAAATCGCCGAACAATGTTTCACTACAAACGACGTCGTCTTCGCCAACCGCCCCCGCCTGCTCATCAGCCGTTACCTGGGCTACAACTGCACCACCATCTCCACCGCCCCGTACGGCGACTACTGGCGGAACCTCCGCCGCCTCTCCGCCGTGGAGATCTTCTCGACGGCAAAACTAAACGCATCTCTGTGCATCCGCAAGGAAGAAATCCGGCGATTCCTCCTGAAACTCCACTCGGAATGGTTCGCCAAAGCGGAGCTGAAATCGATGTTTTCGGAACTGAGCTTCAACGTGGTGATGCGGGTGGTGGCCGGAAAACGGTACTACGGCGAGGAGGTTTCCGACGAGGCGGAGGCGAGGGAGTTCCGGGAGCTGATGGAGGAGATTTCGGCCCAGGGCGGGGCTTCGCATTGGGAGGATTTTATGCCGATCTTGAAATGGATCGGGCGCGGTGGATTCGAGAACAGTTTGAAGAAATCCGGGGAAAGGGCGGATCGGTTTATGCAGGCGTTGATTGAAGAACGCCGGAATTTGAAACTGCTCGGAACTGAAAAACAGAGGAATCTGCTTGATCGGCTTTTGGAGTTGCAGCTTTCTGAGCCGGAATATTACTCCGATCACACAATTAAAGGGATCGTTCTGGTGTTGTTGCGTGCCGGAACAGATACATCATCTGTGACTTTGAATTGGGCAATGACTCAACTACTCAACAATCCGGAAATGTTGGCCAAAGCAAGAGCTGAGATAGACACCAAAATCGGCCAAGATCGCCCCATCAACGAAGGAGATCTCCCCAACCTCGACTACCTCCAAGCCATTATTTCCGAGACCTTCCGCCTTCACCCTGCCGCACCGATGCTCCTCACACACTACTCCTCTGACGATTGCGTCGTTGCAGGCTACGACATTCCTCGTGGGACAACGTTGTTGGTCAATGCCTGGGCCATTCACCGAGACCCGAAGTTGTGGGACGACCCGACGAGCTTCAACCCAGAGAGGTTTTTTGGGCCAGGAAATGAGTTGAACTCAAACAAAATAATTCCTTTTGGGATTGGAAGGAGGGCTTGTCCTGGTGCGAGCATGGCTCAACGTTTCATAGGATTGACTTTGGGGCTATTGATTCAATGCTATGAGTGGAAAAAGGATGGAGAAGAAAATGTGGACATGGCCGAAGGTGGAGGCATTACTATTCATAAAGTCAAGCCTTTGGAAACTATGTGCAAAGCTCGCCCAATTATGGTTAGAGTTCTATCCAATGGTTTGGACTAAATTTGAACAAGGTTTGGTTACTTAGACCTTTCCTCAATGGCTTAACTAAATAATGTAGTTACTACTAGATGGTTCTTATTAAATGAATGTTATAATCACTATGATATATATATATTTTAATAATAACTTCAATATCTAAAAATATCGAAGATTGAGAGCACATTTGAGGGTGAAGGTAGACTTACTACCTTTTGAAACATCTTCAAAATATCTACTAGATGTTTTTTTTTGGGTGGTATTTAGCAATAGTAAGTAATTTTTGAAACATCAAAAAGTTACTTTAAGTAATTTTCAATAAATAATCTAGATGTTGAGTTATTTTGTTTTTAAAGATTTCTTAAAAATAAAATTGAGAAATTGGAATTTCGCCCTAAAGATAAAGTATCAACTATATGCATGAAGCAATGATCATTTAAAAATGGTTGTCCATACTTATTCTGTGTCTCACTTCTTCTAATCAAATTTAATATATAGGAGTATGGATAATTGAAATGTACGTTTACGGATAATTTCAACTTAGATAAAAAAGAATGCAATTCTATTGATTCTAAATATTATTATTTTATGTCTACTTACAAAAAGCAAGGTTTTATTTCTAAAATCTTCACCTATATTGTTTTCATGCAAGCATGTGTTCAATTTCATTTTTAAAATTAAATCTTACAAATTATGCTAATTCAAATGATCAATTTAAGCTAAAGATAAAACTTAATAAAAGTACTAAATAATGTCAAATAAGTTGAGCCAAGTTGTTTAAGCATAAAGTGAGATTTTCTTCCTTCTTAAAATATTACATTTAATTAATTTTCCATGTAAGACAAACTTGAATAAAAAAAATAAAAAATAAAAAAAGACAAACTTGAATAAAAAAAAAATTGAACTTCATTGAAAAAGTTGTGAACTTTTGAAATAACCAACTTAAGTACAAATAACTAAAATTAAAACTAAACTGTACACCTTTTATCCAAAAAAAGAAAAGAAAAGAAAGAAAGAAAGAAAAACAGAACTTACAAAACTCAAACACACTAGAAACATTATCAGCAAACTTTAATACCAGGAAATAGAAATCAGTAATTCCCAGAAAACTTGAATTTACACAGCTGATTTCATCCTATGTAAAGAGACAATTTAAAACCAATAAGGCAATGCATATAAAATATAAAGGAAAAATTGGTCATAATCATAATCTTGAAC

Coding sequence (CDS)

ATGGACTTTCTCACTCTTCTCTTTCTCCTCCTTGTTGCTTTCTTCATCCTCCAACAGCTCAAAACCCGCCGGAAAAATCTGCCGCCGTCTCCGCCGTGTGTTCCGGTCATCGGCCACCTCCACCTCCTCAAACGCCCCATCCACCGGAATTTTCAAACCATCGCCGCCAAGTACGGCCCCATTTTCACCCTCCGACTCGGATCTCGCCTTGCCCTCGTTGTCTCTTCCCTCCAAATCGCCGAACAATGTTTCACTACAAACGACGTCGTCTTCGCCAACCGCCCCCGCCTGCTCATCAGCCGTTACCTGGGCTACAACTGCACCACCATCTCCACCGCCCCGTACGGCGACTACTGGCGGAACCTCCGCCGCCTCTCCGCCGTGGAGATCTTCTCGACGGCAAAACTAAACGCATCTCTGTGCATCCGCAAGGAAGAAATCCGGCGATTCCTCCTGAAACTCCACTCGGAATGGTTCGCCAAAGCGGAGCTGAAATCGATGTTTTCGGAACTGAGCTTCAACGTGGTGATGCGGGTGGTGGCCGGAAAACGGTACTACGGCGAGGAGGTTTCCGACGAGGCGGAGGCGAGGGAGTTCCGGGAGCTGATGGAGGAGATTTCGGCCCAGGGCGGGGCTTCGCATTGGGAGGATTTTATGCCGATCTTGAAATGGATCGGGCGCGGTGGATTCGAGAACAGTTTGAAGAAATCCGGGGAAAGGGCGGATCGGTTTATGCAGGCGTTGATTGAAGAACGCCGGAATTTGAAACTGCTCGGAACTGAAAAACAGAGGAATCTGCTTGATCGGCTTTTGGAGTTGCAGCTTTCTGAGCCGGAATATTACTCCGATCACACAATTAAAGGGATCGTTCTGGTGTTGTTGCGTGCCGGAACAGATACATCATCTGTGACTTTGAATTGGGCAATGACTCAACTACTCAACAATCCGGAAATGTTGGCCAAAGCAAGAGCTGAGATAGACACCAAAATCGGCCAAGATCGCCCCATCAACGAAGGAGATCTCCCCAACCTCGACTACCTCCAAGCCATTATTTCCGAGACCTTCCGCCTTCACCCTGCCGCACCGATGCTCCTCACACACTACTCCTCTGACGATTGCGTCGTTGCAGGCTACGACATTCCTCGTGGGACAACGTTGTTGGTCAATGCCTGGGCCATTCACCGAGACCCGAAGTTGTGGGACGACCCGACGAGCTTCAACCCAGAGAGGTTTTTTGGGCCAGGAAATGAGTTGAACTCAAACAAAATAATTCCTTTTGGGATTGGAAGGAGGGCTTGTCCTGGTGCGAGCATGGCTCAACGTTTCATAGGATTGACTTTGGGGCTATTGATTCAATGCTATGAGTGGAAAAAGGATGGAGAAGAAAATGTGGACATGGCCGAAGGTGGAGGCATTACTATTCATAAAGTCAAGCCTTTGGAAACTATGTGCAAAGCTCGCCCAATTATGGTTAGAGTTCTATCCAATGGTTTGGACTAA

Protein sequence

MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGPIFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWRNLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAKAELKSMFSELSFNVVMRVVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSGERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAGTDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRLHPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNELNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKVKPLETMCKARPIMVRVLSNGLD
Homology
BLAST of Sed0020417 vs. NCBI nr
Match: AEM42992.1 (cytochrome P450 [Siraitia grosvenorii])

HSP 1 Score: 785.0 bits (2026), Expect = 3.8e-223
Identity = 379/502 (75.50%), Postives = 436/502 (86.85%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           MDF +   LLL+   IL Q++TRR+NLPPSPP +P+IGHLHLLKRPIHRNF  IAA+YGP
Sbjct: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IF+LR GSRLA++VSSL IAE+CFT ND++FANRPRLLIS++LGYNCTT++T+PYGD+WR
Sbjct: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLH---SEWFAKAELKSMFSELSFNVVM 180
           NLRRL+A+EIFSTA+LN+SL IRK+EI+R LLKLH   S  F K ELK+MFSEL+FN +M
Sbjct: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180

Query: 181 RVVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKS 240
           R+VAGKRYYG+EVSDE EAREFR LMEEIS  GGASHW DFMPILKWIG GGFE SL + 
Sbjct: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240

Query: 241 GERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAG 300
            +R D+FMQALIEERRN K+L  E++ +LLDRLLELQ SEPEYY+D  IKG+VLVLLRAG
Sbjct: 241 TKRTDKFMQALIEERRNKKVL--ERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300

Query: 301 TDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRL 360
           TDTS+VTLNWAM QLLNNPE+LAKA+AE+DTKIGQDRP++E DLPNL YLQAI+SET RL
Sbjct: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360

Query: 361 HPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNE 420
           HPAAPMLL+HYSS DC VAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSF PERF G  NE
Sbjct: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420

Query: 421 LNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKV 480
           L S K+I FG+GRR+CPG +MA RF+GLTLGLLIQCY+WKK G+E VDM EGGGITIHK 
Sbjct: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480

Query: 481 KPLETMCKARPIMVRVLSNGLD 500
           KPLE MCKARP M ++L N LD
Sbjct: 481 KPLEAMCKARPAMYKLLLNALD 500

BLAST of Sed0020417 vs. NCBI nr
Match: XP_023512643.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 784.3 bits (2024), Expect = 6.4e-223
Identity = 383/499 (76.75%), Postives = 433/499 (86.77%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           MDFL++L LLL+ F  LQQ  T R+NLPPSPP VP+IGHLHLL+RPIHRNF  IAAKYGP
Sbjct: 1   MDFLSVLLLLLLVFLFLQQFITCRRNLPPSPPSVPIIGHLHLLQRPIHRNFHHIAAKYGP 60

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IFTLR GSRLAL+VSSLQ+AE+CFT NDVVFANRPRLLI +YLGYN TTIST PYGD+WR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDHWR 120

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAKAELKSMFSELSFNVVMRVV 180
           NLRRL+++EIFSTA+LNA+L IRK+EIRR L+KLHSE FA+ ELKSMFSELSFNVVMRVV
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFARVELKSMFSELSFNVVMRVV 180

Query: 181 AGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSGER 240
            GKRY+GEEVS+E EAREFRELM+E S  GGAS+W DFMPILKWIG G +E SL K  +R
Sbjct: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWIDFMPILKWIGFGEYEKSLAKLTKR 240

Query: 241 ADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAGTDT 300
           AD+FMQ L+E+RRN   L  EKQ  LLDRLLELQ SEPEYYSD  IKG++LVLLRAG D+
Sbjct: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300

Query: 301 SSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRLHPA 360
           +SVT+ WAMTQLLNNPE+++KA+AEIDTKIG+DR ++E DL NL YLQAIISETFRLHPA
Sbjct: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDEADLTNLHYLQAIISETFRLHPA 360

Query: 361 APMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNELNS 420
           APMLLTHYSSDDC VAGYDIPRGT LLVNAWAIHRDPKLWDDP  F PERF G GNEL S
Sbjct: 361 APMLLTHYSSDDCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPMRFRPERFLGEGNELLS 420

Query: 421 NKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKVKPL 480
           NK+IPFG+GRRACPG +MAQR IGL+LGLLIQCY+WK  G + VDM EGGGITI KV+PL
Sbjct: 421 NKLIPFGMGRRACPGETMAQRLIGLSLGLLIQCYDWKNVGYQKVDMTEGGGITIQKVQPL 480

Query: 481 ETMCKARPIMVRVLSNGLD 500
           ET+C+A PIM +VLSNGLD
Sbjct: 481 ETLCRALPIMDKVLSNGLD 499

BLAST of Sed0020417 vs. NCBI nr
Match: XP_022944057.1 (cytochrome P450 81E8-like [Cucurbita moschata] >KAG7011030.1 Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 783.1 bits (2021), Expect = 1.4e-222
Identity = 384/499 (76.95%), Postives = 431/499 (86.37%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           MDFL +L LLL+ F  LQQ  TRR+NLPPSPP  P+IGHLHLL+RPIHRNF  IAAKYGP
Sbjct: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IFTLR GSRLAL+VSSLQ+AE+CFT NDVVFANRPRLLI +YLGYN TTIST P+GD+WR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAKAELKSMFSELSFNVVMRVV 180
           NLRRL+++EIFSTA+LNA+L IRK+EIRR L+KLHSE FAK ELKSMFSELSFNVVMRVV
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180

Query: 181 AGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSGER 240
            GKRY+GEEVS+E EAREFRELM+E S  GGAS+W DFMPILKWIG G +E SL K  +R
Sbjct: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240

Query: 241 ADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAGTDT 300
           AD+FMQ L+E+RRN   L  EKQ  LLDRLLELQ SEPEYYSD  IKG++LVLLRAG D+
Sbjct: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300

Query: 301 SSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRLHPA 360
           +SVT+ WAMTQLLNNPE+++KA+AEIDTKIG+DR I+E DL NL YLQAIISETFRLHPA
Sbjct: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360

Query: 361 APMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNELNS 420
           APMLLTHYSS+DC VAGYD+PRGT LLVNAWAIHRDPKLWDDP SF PERF G GNEL  
Sbjct: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420

Query: 421 NKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKVKPL 480
           NK+IPFG+GRRACPG +MA R IGL+LGLLIQCYEWK  G E VDM EGGGITI KV+PL
Sbjct: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480

Query: 481 ETMCKARPIMVRVLSNGLD 500
           ETMC+A PIM +VLSNGLD
Sbjct: 481 ETMCRALPIMDKVLSNGLD 499

BLAST of Sed0020417 vs. NCBI nr
Match: XP_022985607.1 (cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E8-like [Cucurbita maxima])

HSP 1 Score: 782.7 bits (2020), Expect = 1.9e-222
Identity = 382/499 (76.55%), Postives = 431/499 (86.37%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           MDFL++  LLL+ F  LQQ  TRR+NLPPSPP VP+IGHLHLL+RPIH+NF  IAAKYGP
Sbjct: 1   MDFLSVFLLLLLVFLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAKYGP 60

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IFTLR GSRLAL+VSSLQ+AE+CFT NDVVFANRPRLLI +YLGYN TTIST PYGD WR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDLWR 120

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAKAELKSMFSELSFNVVMRVV 180
           NLRRL+++E+FSTA+LNA+L IRK+EIRR L+KLHSE FAK ELKSMFSELSFNVVMRVV
Sbjct: 121 NLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180

Query: 181 AGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSGER 240
            GKRY+GEEVS+E EAREFRELM+EIS  GGAS+W +FMPILKWIG G +E SL K  +R
Sbjct: 181 TGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPILKWIGFGEYEKSLAKLTKR 240

Query: 241 ADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAGTDT 300
           AD+FMQ L+E RRN      EKQ  LLDRLLELQ SEPEYYSD  IKG+VLVLLRAG D+
Sbjct: 241 ADKFMQKLVEYRRNQTGFEIEKQSTLLDRLLELQASEPEYYSDEIIKGVVLVLLRAGVDS 300

Query: 301 SSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRLHPA 360
           +SVT+ WAMTQLLNNPE+++KA+AEIDTKIG+DR ++E DL NL YLQAII ETFRLHPA
Sbjct: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETFRLHPA 360

Query: 361 APMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNELNS 420
           APMLLTHYSSDDC VAGYDIPRGT L+VNAWAIHRDPKLWDDP SF PERF G GNEL S
Sbjct: 361 APMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAGNELLS 420

Query: 421 NKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKVKPL 480
           NK++PFG+GRRACPG +MAQR IGL+LGLLIQCYEWK  G + VDM EGGGITI KV+PL
Sbjct: 421 NKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQKVQPL 480

Query: 481 ETMCKARPIMVRVLSNGLD 500
           ETMC+A PIM +VLSNGLD
Sbjct: 481 ETMCRALPIMDKVLSNGLD 499

BLAST of Sed0020417 vs. NCBI nr
Match: XP_008455812.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo] >KAA0032504.1 cytochrome P450 81E8-like [Cucumis melo var. makuwa])

HSP 1 Score: 773.9 bits (1997), Expect = 8.7e-220
Identity = 377/499 (75.55%), Postives = 430/499 (86.17%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           MDFL+  FLL ++F +LQQL+TRR+NLPPSPP  P+IGHLHLL+RP HRNFQ IAAKYGP
Sbjct: 1   MDFLSAFFLLFLSFLLLQQLRTRRRNLPPSPPSFPIIGHLHLLQRPTHRNFQNIAAKYGP 60

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IF+LR GSRLA++VSSLQIA++CFT +D++FANRPRLL  +Y GY+ TT++TA YGD+WR
Sbjct: 61  IFSLRFGSRLAVIVSSLQIAQECFTKHDLIFANRPRLLSGKYFGYSWTTMTTASYGDHWR 120

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAKAELKSMFSELSFNVVMRVV 180
           NLRRL+A+EIFS  +LNASL  RK+EIRR L+KLHSE FAK EL  MFSELSFN+VMRVV
Sbjct: 121 NLRRLAAMEIFSPTRLNASLAFRKDEIRRLLVKLHSESFAKVELTPMFSELSFNIVMRVV 180

Query: 181 AGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSGER 240
           AGKRYYGE+VSDEAEAREFRELM+E+S QGG S W DFMPILKWIG G +E  L KS   
Sbjct: 181 AGKRYYGEKVSDEAEAREFRELMDEVSRQGGTSQWVDFMPILKWIGFGAYEKILTKSAIW 240

Query: 241 ADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAGTDT 300
           ADRF+Q LIEE RN K+LG E+Q +LL RLLELQLS+PEYY+D  IKG+VLVLLRAG DT
Sbjct: 241 ADRFVQELIEEHRNKKVLGREEQSSLLHRLLELQLSQPEYYTDQIIKGLVLVLLRAGIDT 300

Query: 301 SSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRLHPA 360
           SSVTL+WAMT+LLN+PE+LAKA+AEIDTKIGQDRP+ E D+ NL+YLQAIISETFRLHP 
Sbjct: 301 SSVTLDWAMTELLNHPEVLAKAKAEIDTKIGQDRPVEESDIANLNYLQAIISETFRLHPP 360

Query: 361 APMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNELNS 420
           APMLLTHYSSDDC VAGY+IPRGT LLVNA AIHRDPKLWDDPTSF PERF G GNEL +
Sbjct: 361 APMLLTHYSSDDCCVAGYNIPRGTMLLVNASAIHRDPKLWDDPTSFRPERFLGAGNELQT 420

Query: 421 NKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKVKPL 480
           NK+IPFGIGRRACPG  M  R +GLTLGLLIQCYEWKK G ENVD  E GGITI KVKP+
Sbjct: 421 NKLIPFGIGRRACPGEIMGLRVVGLTLGLLIQCYEWKKVGYENVDTTEYGGITILKVKPV 480

Query: 481 ETMCKARPIMVRVLSNGLD 500
           ETMCK  P+MV+VLSNGLD
Sbjct: 481 ETMCKPCPVMVKVLSNGLD 499

BLAST of Sed0020417 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.5e-153
Identity = 276/504 (54.76%), Postives = 361/504 (71.63%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLK-----TRRKNLPPSPP-CVPVIGHLHLLKRPIHRNFQTI 60
           M+  TLL+  L    +   LK      RR+NLPPSP   +PVIGHLHL+ + +HR+   +
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60

Query: 61  AAKYGPIFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAP 120
           + KYG +F+L+LG+RL LVVSS   AE+CFT ND+VFANRP  ++ +Y+GYN TT+  +P
Sbjct: 61  SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120

Query: 121 YGDYWRNLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLH---SEWFAKAELKSMFSEL 180
           YG++WRNLRRL+AVEIFS   LN  L IR++E+++ LL L+    + F K E+KS  SEL
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSEL 180

Query: 181 SFNVVMRVVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFE 240
           SFNV MR+VAGKRY+G++V D  EA+ FR L+ E+    GAS+  DF+P L+WI    +E
Sbjct: 181 SFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYE 240

Query: 241 NSLKKSGERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVL 300
             + K  +  D F+Q LI E R  K         ++D LL LQ S+PEYY+D  IKGI++
Sbjct: 241 KKVSKISQEMDAFLQRLIHESRINK-----NNVTMIDHLLSLQESQPEYYTDQIIKGIIM 300

Query: 301 VLLRAGTDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAII 360
           VLL AGTDTS+VT+ WAM+ LLN+PE L KAR EI+T++G +R I E DLP L YL  II
Sbjct: 301 VLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNII 360

Query: 361 SETFRLHPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERF 420
           SETFRL PAAPML+ H SSDDC V GYD+P+GT LLVNAWAIHRDP+ WD+PT F PER 
Sbjct: 361 SETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH 420

Query: 421 FGPGNELNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGG 480
              G EL  +K++PFG+GRR+CPG+ +AQR +GLTLG LIQC+EWK+ GE  +DMAEG G
Sbjct: 421 --GGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSG 480

Query: 481 ITIHKVKPLETMCKARPIMVRVLS 496
           +T+ K +PLE +CK R I+ +V+S
Sbjct: 481 LTMPKAQPLEALCKPRNILHKVVS 496

BLAST of Sed0020417 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 2.0e-147
Identity = 254/492 (51.63%), Postives = 344/492 (69.92%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           +  +  LF L++   +      + KNLPP P C+P+IG+LH LK+P+H  F T++ KYG 
Sbjct: 6   LSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQ 65

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IF+L  GSRL +VVSSL IA++CFT ND+V ANRP  L  +Y+GYN TT++ +PYGD+WR
Sbjct: 66  IFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWR 125

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSE---WFAKAELKSMFSELSFNVVM 180
           NLRR+ ++EI S+ +LN+ L IR++EI R + KL  +    F + EL+ MFSE++FN +M
Sbjct: 126 NLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIM 185

Query: 181 RVVAGKRYYGE--EVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLK 240
           R+V+GKRYYG   +VSD  EAR FR +++E+ + GGA++  DF+  L+W    G E  LK
Sbjct: 186 RMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLK 245

Query: 241 KSGERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLR 300
           K  +R D F+Q LI+E R     G      ++D LL  Q S+PEYY+D  IKG+++V+L 
Sbjct: 246 KISKRTDAFLQGLIDEHR----FGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLL 305

Query: 301 AGTDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETF 360
           AGTDTSSVT+ WAM+ LLN+PE++ KA+ E+DT IG DR ++E D+  L YLQ+I+ ET 
Sbjct: 306 AGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETL 365

Query: 361 RLHPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPG 420
           RLH AAP+L+ H SS+D  + GY+IP+ T L+VNAW IHRDP LW DPT F PERF   G
Sbjct: 366 RLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEG 425

Query: 421 NELNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIH 480
                NK++ FG+GRRACPG +++QR  GLTLGLLIQC+EWK+ GEE +DM E  GIT  
Sbjct: 426 ---EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAG 485

Query: 481 KVKPLETMCKAR 488
           K   L  MCK R
Sbjct: 486 KKTSLNAMCKVR 490

BLAST of Sed0020417 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 4.9e-141
Identity = 249/499 (49.90%), Postives = 348/499 (69.74%), Query Frame = 0

Query: 7   LFLLLVAFFILQQLKTRRKNLPPSPP-CVPVIGHLHLLKRPIHRNFQTIAAKY-----GP 66
           +F L+      + LK +++NLPPSPP  +P+IGHL LLK PIHR  ++ +        G 
Sbjct: 13  IFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGG 72

Query: 67  IFTLRLGSRLALVVSSLQI-AEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYW 126
           + +LRLGSRL  VVSS ++ AE+CF  NDVV ANRP+++I +++GYN T +  APYGD+W
Sbjct: 73  VMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHW 132

Query: 127 RNLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAK---AELKSMFSELSFNVV 186
           RNLRRL  +EIFST +LN  L +R +E+RR + +L      K    ELK M  +L+FN +
Sbjct: 133 RNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNI 192

Query: 187 MRVVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKK 246
           MR++ GKRYYGEE +DE EA+  R+L+ ++ A   + +  D++PIL+      +EN +KK
Sbjct: 193 MRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENRVKK 252

Query: 247 SGERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRA 306
            GE  D+F+Q LI+++R  +  GT     ++D LL LQ S+ EYY+D  IKGI+L+++ A
Sbjct: 253 LGEETDKFLQGLIDDKRGQQETGT----TMIDHLLVLQKSDIEYYTDQIIKGIILIMVIA 312

Query: 307 GTDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFR 366
           GT+TS+VTL WA++ LLN+P++++KAR EID ++G DR I E DL  L YL+ I+ ET R
Sbjct: 313 GTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLR 372

Query: 367 LHPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGN 426
           LHPA P+L+ H +S+DC +  YD+PRGTTLLVNAWAIHRDP  WDDP SF PERF     
Sbjct: 373 LHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERF---EK 432

Query: 427 ELNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHK 486
           E  + K++ FG+GRRACPG+ +AQR +GL LG LIQC+EW++ G   VDM EG G T+ K
Sbjct: 433 EEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPK 492

Query: 487 VKPLETMCKARPIMVRVLS 496
             PL+ +CKARP + +++S
Sbjct: 493 AIPLKAICKARPFLHKIIS 502

BLAST of Sed0020417 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 5.4e-140
Identity = 241/495 (48.69%), Postives = 348/495 (70.30%), Query Frame = 0

Query: 4   LTLLFLLLVAFFILQQLKTRR-KNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGPIF 63
           L+L F++ +   IL ++ +RR KNLPP PP +P+IG+LH LK P+HR F T++  YG IF
Sbjct: 8   LSLSFIITIK--ILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIF 67

Query: 64  TLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWRNL 123
           +L  GSRL +VVSS  +A +CFT ND++ ANRPR L  +Y+ YN TT+ +A YGD+WRNL
Sbjct: 68  SLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNL 127

Query: 124 RRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSE------WFAKAELKSMFSELSFNVV 183
           RR++ +++ S  +LN+ L +R++E  R + KL  +       F K EL+   +E++FN +
Sbjct: 128 RRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAM 187

Query: 184 MRVVAGKRYYGE--EVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSL 243
           MR+++GKRYYG+  +VSD  EA++FRE++ E+ +  GA++  DF+P+L+ +     E   
Sbjct: 188 MRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRC 247

Query: 244 KKSGERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLL 303
           K+  +R++ F++ LIEE R   +        ++D LL+L  S+PEYYSDH IKG++  +L
Sbjct: 248 KRIAKRSNAFLEGLIEEHRRGNI--HSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGML 307

Query: 304 RAGTDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISET 363
            AGTDTS+VT+ W M++LLN+PE+L KA+ E+DT+IG+++ ++E DL  L YLQ IISET
Sbjct: 308 LAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISET 367

Query: 364 FRLHPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGP 423
            RLHP AP+LL HYSS+DC +  +++P+ T +L N W IHRDPK W+D  SF PERF   
Sbjct: 368 LRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF--- 427

Query: 424 GNELNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITI 483
             E   NK++ FG+GRRACPG S+AQR +G T+GLLIQC+EW+++ EE +DM EG GIT+
Sbjct: 428 EKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITM 487

Query: 484 HKVKPLETMCKARPI 490
               PL  MCKA PI
Sbjct: 488 PMKIPLRAMCKALPI 495

BLAST of Sed0020417 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 4.6e-139
Identity = 252/501 (50.30%), Postives = 345/501 (68.86%), Query Frame = 0

Query: 7   LFLLLVAFFILQQLK------TRRKNLPPSPP-CVPVIGHLHLLKRPIHRNFQTIAAKY- 66
           L L L  FFI   LK       R+ NLPPSP    P+IGHLHLLK P+HR F +++    
Sbjct: 13  LILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLN 72

Query: 67  -GPIFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGD 126
              IF+L LGSRL  VVSS  +AE+CFT NDVV ANRP  L+ +++GYN TT+  A YGD
Sbjct: 73  NAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGD 132

Query: 127 YWRNLRRLSAVEIFSTAKLNASLCIRKEEIRRFLL---KLHSEWFAKAELKSMFSELSFN 186
            WRNLRR+  +EIFS+ +LN+ + IR++EIRR ++   K     F K E+K +F  L+ N
Sbjct: 133 SWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTIN 192

Query: 187 VVMRVVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSL 246
            ++R+VAGKR+YG+   ++ EA+  R+L+ E+   GGA +  D+ PIL+++    +E  +
Sbjct: 193 NIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEKHV 252

Query: 247 KKSGERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLL 306
           KK   R D F+Q+L+ E+R  K+ G      ++D LL LQ ++P+YY+D  IKGI+LV++
Sbjct: 253 KKLAGRVDEFLQSLVNEKRVEKVKG----NTMIDHLLSLQETQPDYYTDVIIKGIILVMI 312

Query: 307 RAGTDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISET 366
            AGTDTS+ TL WAM+ LLN+PE+L KA+ EID +IG DR + E D+  L YLQ I+SET
Sbjct: 313 LAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSET 372

Query: 367 FRLHPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGP 426
            RL+P APMLL H +S+DC+V GYD+PRGT +LVNAWAIHRDPKLW++P  F PERF   
Sbjct: 373 LRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKK 432

Query: 427 GNELNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEG-GGIT 486
           G +    K++PFGIGRR+CPG+ +AQR + L LG L+QC+EW++  E+ +DM E   G T
Sbjct: 433 GED---KKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTT 492

Query: 487 IHKVKPLETMCKARPIMVRVL 495
           + K   L+ MCKARPI+ +VL
Sbjct: 493 MRKATSLQAMCKARPIVHKVL 504

BLAST of Sed0020417 vs. ExPASy TrEMBL
Match: K7NC01 (Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.8e-223
Identity = 379/502 (75.50%), Postives = 436/502 (86.85%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           MDF +   LLL+   IL Q++TRR+NLPPSPP +P+IGHLHLLKRPIHRNF  IAA+YGP
Sbjct: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IF+LR GSRLA++VSSL IAE+CFT ND++FANRPRLLIS++LGYNCTT++T+PYGD+WR
Sbjct: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLH---SEWFAKAELKSMFSELSFNVVM 180
           NLRRL+A+EIFSTA+LN+SL IRK+EI+R LLKLH   S  F K ELK+MFSEL+FN +M
Sbjct: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180

Query: 181 RVVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKS 240
           R+VAGKRYYG+EVSDE EAREFR LMEEIS  GGASHW DFMPILKWIG GGFE SL + 
Sbjct: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240

Query: 241 GERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAG 300
            +R D+FMQALIEERRN K+L  E++ +LLDRLLELQ SEPEYY+D  IKG+VLVLLRAG
Sbjct: 241 TKRTDKFMQALIEERRNKKVL--ERKNSLLDRLLELQASEPEYYTDQIIKGLVLVLLRAG 300

Query: 301 TDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRL 360
           TDTS+VTLNWAM QLLNNPE+LAKA+AE+DTKIGQDRP++E DLPNL YLQAI+SET RL
Sbjct: 301 TDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRL 360

Query: 361 HPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNE 420
           HPAAPMLL+HYSS DC VAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSF PERF G  NE
Sbjct: 361 HPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANE 420

Query: 421 LNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKV 480
           L S K+I FG+GRR+CPG +MA RF+GLTLGLLIQCY+WKK G+E VDM EGGGITIHK 
Sbjct: 421 LQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKA 480

Query: 481 KPLETMCKARPIMVRVLSNGLD 500
           KPLE MCKARP M ++L N LD
Sbjct: 481 KPLEAMCKARPAMYKLLLNALD 500

BLAST of Sed0020417 vs. ExPASy TrEMBL
Match: A0A6J1FUQ9 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 6.9e-223
Identity = 384/499 (76.95%), Postives = 431/499 (86.37%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           MDFL +L LLL+ F  LQQ  TRR+NLPPSPP  P+IGHLHLL+RPIHRNF  IAAKYGP
Sbjct: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IFTLR GSRLAL+VSSLQ+AE+CFT NDVVFANRPRLLI +YLGYN TTIST P+GD+WR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAKAELKSMFSELSFNVVMRVV 180
           NLRRL+++EIFSTA+LNA+L IRK+EIRR L+KLHSE FAK ELKSMFSELSFNVVMRVV
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180

Query: 181 AGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSGER 240
            GKRY+GEEVS+E EAREFRELM+E S  GGAS+W DFMPILKWIG G +E SL K  +R
Sbjct: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240

Query: 241 ADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAGTDT 300
           AD+FMQ L+E+RRN   L  EKQ  LLDRLLELQ SEPEYYSD  IKG++LVLLRAG D+
Sbjct: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300

Query: 301 SSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRLHPA 360
           +SVT+ WAMTQLLNNPE+++KA+AEIDTKIG+DR I+E DL NL YLQAIISETFRLHPA
Sbjct: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360

Query: 361 APMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNELNS 420
           APMLLTHYSS+DC VAGYD+PRGT LLVNAWAIHRDPKLWDDP SF PERF G GNEL  
Sbjct: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420

Query: 421 NKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKVKPL 480
           NK+IPFG+GRRACPG +MA R IGL+LGLLIQCYEWK  G E VDM EGGGITI KV+PL
Sbjct: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480

Query: 481 ETMCKARPIMVRVLSNGLD 500
           ETMC+A PIM +VLSNGLD
Sbjct: 481 ETMCRALPIMDKVLSNGLD 499

BLAST of Sed0020417 vs. ExPASy TrEMBL
Match: A0A6J1J5B5 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 9.0e-223
Identity = 382/499 (76.55%), Postives = 431/499 (86.37%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           MDFL++  LLL+ F  LQQ  TRR+NLPPSPP VP+IGHLHLL+RPIH+NF  IAAKYGP
Sbjct: 1   MDFLSVFLLLLLVFLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAKYGP 60

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IFTLR GSRLAL+VSSLQ+AE+CFT NDVVFANRPRLLI +YLGYN TTIST PYGD WR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDLWR 120

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAKAELKSMFSELSFNVVMRVV 180
           NLRRL+++E+FSTA+LNA+L IRK+EIRR L+KLHSE FAK ELKSMFSELSFNVVMRVV
Sbjct: 121 NLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180

Query: 181 AGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSGER 240
            GKRY+GEEVS+E EAREFRELM+EIS  GGAS+W +FMPILKWIG G +E SL K  +R
Sbjct: 181 TGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPILKWIGFGEYEKSLAKLTKR 240

Query: 241 ADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAGTDT 300
           AD+FMQ L+E RRN      EKQ  LLDRLLELQ SEPEYYSD  IKG+VLVLLRAG D+
Sbjct: 241 ADKFMQKLVEYRRNQTGFEIEKQSTLLDRLLELQASEPEYYSDEIIKGVVLVLLRAGVDS 300

Query: 301 SSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRLHPA 360
           +SVT+ WAMTQLLNNPE+++KA+AEIDTKIG+DR ++E DL NL YLQAII ETFRLHPA
Sbjct: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETFRLHPA 360

Query: 361 APMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNELNS 420
           APMLLTHYSSDDC VAGYDIPRGT L+VNAWAIHRDPKLWDDP SF PERF G GNEL S
Sbjct: 361 APMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAGNELLS 420

Query: 421 NKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKVKPL 480
           NK++PFG+GRRACPG +MAQR IGL+LGLLIQCYEWK  G + VDM EGGGITI KV+PL
Sbjct: 421 NKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQKVQPL 480

Query: 481 ETMCKARPIMVRVLSNGLD 500
           ETMC+A PIM +VLSNGLD
Sbjct: 481 ETMCRALPIMDKVLSNGLD 499

BLAST of Sed0020417 vs. ExPASy TrEMBL
Match: A0A5A7SMV7 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00110 PE=3 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 4.2e-220
Identity = 377/499 (75.55%), Postives = 430/499 (86.17%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           MDFL+  FLL ++F +LQQL+TRR+NLPPSPP  P+IGHLHLL+RP HRNFQ IAAKYGP
Sbjct: 1   MDFLSAFFLLFLSFLLLQQLRTRRRNLPPSPPSFPIIGHLHLLQRPTHRNFQNIAAKYGP 60

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IF+LR GSRLA++VSSLQIA++CFT +D++FANRPRLL  +Y GY+ TT++TA YGD+WR
Sbjct: 61  IFSLRFGSRLAVIVSSLQIAQECFTKHDLIFANRPRLLSGKYFGYSWTTMTTASYGDHWR 120

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAKAELKSMFSELSFNVVMRVV 180
           NLRRL+A+EIFS  +LNASL  RK+EIRR L+KLHSE FAK EL  MFSELSFN+VMRVV
Sbjct: 121 NLRRLAAMEIFSPTRLNASLAFRKDEIRRLLVKLHSESFAKVELTPMFSELSFNIVMRVV 180

Query: 181 AGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSGER 240
           AGKRYYGE+VSDEAEAREFRELM+E+S QGG S W DFMPILKWIG G +E  L KS   
Sbjct: 181 AGKRYYGEKVSDEAEAREFRELMDEVSRQGGTSQWVDFMPILKWIGFGAYEKILTKSAIW 240

Query: 241 ADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAGTDT 300
           ADRF+Q LIEE RN K+LG E+Q +LL RLLELQLS+PEYY+D  IKG+VLVLLRAG DT
Sbjct: 241 ADRFVQELIEEHRNKKVLGREEQSSLLHRLLELQLSQPEYYTDQIIKGLVLVLLRAGIDT 300

Query: 301 SSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRLHPA 360
           SSVTL+WAMT+LLN+PE+LAKA+AEIDTKIGQDRP+ E D+ NL+YLQAIISETFRLHP 
Sbjct: 301 SSVTLDWAMTELLNHPEVLAKAKAEIDTKIGQDRPVEESDIANLNYLQAIISETFRLHPP 360

Query: 361 APMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNELNS 420
           APMLLTHYSSDDC VAGY+IPRGT LLVNA AIHRDPKLWDDPTSF PERF G GNEL +
Sbjct: 361 APMLLTHYSSDDCCVAGYNIPRGTMLLVNASAIHRDPKLWDDPTSFRPERFLGAGNELQT 420

Query: 421 NKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKVKPL 480
           NK+IPFGIGRRACPG  M  R +GLTLGLLIQCYEWKK G ENVD  E GGITI KVKP+
Sbjct: 421 NKLIPFGIGRRACPGEIMGLRVVGLTLGLLIQCYEWKKVGYENVDTTEYGGITILKVKPV 480

Query: 481 ETMCKARPIMVRVLSNGLD 500
           ETMCK  P+MV+VLSNGLD
Sbjct: 481 ETMCKPCPVMVKVLSNGLD 499

BLAST of Sed0020417 vs. ExPASy TrEMBL
Match: A0A1S3C1R2 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103495911 PE=3 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 4.2e-220
Identity = 377/499 (75.55%), Postives = 430/499 (86.17%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPPCVPVIGHLHLLKRPIHRNFQTIAAKYGP 60
           MDFL+  FLL ++F +LQQL+TRR+NLPPSPP  P+IGHLHLL+RP HRNFQ IAAKYGP
Sbjct: 1   MDFLSAFFLLFLSFLLLQQLRTRRRNLPPSPPSFPIIGHLHLLQRPTHRNFQNIAAKYGP 60

Query: 61  IFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWR 120
           IF+LR GSRLA++VSSLQIA++CFT +D++FANRPRLL  +Y GY+ TT++TA YGD+WR
Sbjct: 61  IFSLRFGSRLAVIVSSLQIAQECFTKHDLIFANRPRLLSGKYFGYSWTTMTTASYGDHWR 120

Query: 121 NLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAKAELKSMFSELSFNVVMRVV 180
           NLRRL+A+EIFS  +LNASL  RK+EIRR L+KLHSE FAK EL  MFSELSFN+VMRVV
Sbjct: 121 NLRRLAAMEIFSPTRLNASLAFRKDEIRRLLVKLHSESFAKVELTPMFSELSFNIVMRVV 180

Query: 181 AGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSGER 240
           AGKRYYGE+VSDEAEAREFRELM+E+S QGG S W DFMPILKWIG G +E  L KS   
Sbjct: 181 AGKRYYGEKVSDEAEAREFRELMDEVSRQGGTSQWVDFMPILKWIGFGAYEKILTKSAIW 240

Query: 241 ADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAGTDT 300
           ADRF+Q LIEE RN K+LG E+Q +LL RLLELQLS+PEYY+D  IKG+VLVLLRAG DT
Sbjct: 241 ADRFVQELIEEHRNKKVLGREEQSSLLHRLLELQLSQPEYYTDQIIKGLVLVLLRAGIDT 300

Query: 301 SSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRLHPA 360
           SSVTL+WAMT+LLN+PE+LAKA+AEIDTKIGQDRP+ E D+ NL+YLQAIISETFRLHP 
Sbjct: 301 SSVTLDWAMTELLNHPEVLAKAKAEIDTKIGQDRPVEESDIANLNYLQAIISETFRLHPP 360

Query: 361 APMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNELNS 420
           APMLLTHYSSDDC VAGY+IPRGT LLVNA AIHRDPKLWDDPTSF PERF G GNEL +
Sbjct: 361 APMLLTHYSSDDCCVAGYNIPRGTMLLVNASAIHRDPKLWDDPTSFRPERFLGAGNELQT 420

Query: 421 NKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKVKPL 480
           NK+IPFGIGRRACPG  M  R +GLTLGLLIQCYEWKK G ENVD  E GGITI KVKP+
Sbjct: 421 NKLIPFGIGRRACPGEIMGLRVVGLTLGLLIQCYEWKKVGYENVDTTEYGGITILKVKPV 480

Query: 481 ETMCKARPIMVRVLSNGLD 500
           ETMCK  P+MV+VLSNGLD
Sbjct: 481 ETMCKPCPVMVKVLSNGLD 499

BLAST of Sed0020417 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 530.8 bits (1366), Expect = 1.2e-150
Identity = 266/500 (53.20%), Postives = 365/500 (73.00%), Query Frame = 0

Query: 5   TLLFLLLVAFFILQQLKTRRKNLPPSPP-CVPVIGHLHLLKRPIHRNFQTIAAKY--GPI 64
           ++LF++L   +++ +LK R+ NLPPSP   +PVIGHL LLK PIHR F +++      PI
Sbjct: 9   SILFVVLSLIYLIGKLK-RKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPI 68

Query: 65  FTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWRN 124
           F+LRLG+RL  V SS  IAE+CFT NDVV ANRP  ++++++ Y+ TT+  A YGD+WRN
Sbjct: 69  FSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRN 128

Query: 125 LRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKL---HSEWFAKAELKSMFSELSFNVVMR 184
           LRR+ +VEIFS  +LN+ L IRK+EIRR + +L    S+ F K ++KSM S+L+FN ++R
Sbjct: 129 LRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILR 188

Query: 185 VVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSG 244
           +VAGKRYYG+ V D+ EA+  R+L+ ++ A  GA +  D++P+L+ +    +E  +KK  
Sbjct: 189 MVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLA 248

Query: 245 ERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRAGT 304
            R D F+Q L++E+R  K    EK   ++D LL LQ S+P+Y++D  IKG +L L+ AGT
Sbjct: 249 GRLDEFLQGLVDEKREAK----EKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGT 308

Query: 305 DTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRLH 364
           DTS+VTL WA++ +LN+P++L KAR EID KIG DR ++E D+ NL YLQ I+SET RL+
Sbjct: 309 DTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLY 368

Query: 365 PAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNEL 424
           PAAPMLL H +S+DC VAGYD+PRGT LL N WAIHRDP+LWDDP SF PERF   G   
Sbjct: 369 PAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEG--- 428

Query: 425 NSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHKVK 484
            + K++PFG+GRRACPG+ +A R I LTLG LIQC EW+K GEE VDM+EG G+T+ K K
Sbjct: 429 EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAK 488

Query: 485 PLETMCKARPIMVRVLSNGL 499
           PLE MC+ARP +V++ +  +
Sbjct: 489 PLEAMCRARPSVVKIFNESV 497

BLAST of Sed0020417 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 517.3 bits (1331), Expect = 1.4e-146
Identity = 267/499 (53.51%), Postives = 356/499 (71.34%), Query Frame = 0

Query: 5   TLLFLLLVAFFILQQLKTRRKNLPPSPP-CVPVIGHLHLLKRPIHRNFQTIAAKYG--PI 64
           T LF+ L   FI+ ++K RR NLPPSP   +PVIGHL LLK P+HR F +++   G  PI
Sbjct: 9   TFLFISLSLTFIIGRIK-RRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPI 68

Query: 65  FTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYWRN 124
            +LRLG+RL  VVSS  +AE+CFT NDVV ANR   L S+++ Y CTT+ TA YGD+WRN
Sbjct: 69  ISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRN 128

Query: 125 LRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKL---HSEWFAKAELKSMFSELSFNVVMR 184
           LRR+ AVEIFS  +LN+   IR++EI R +  L    S  F K E+KSMFS L+FN ++R
Sbjct: 129 LRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIR 188

Query: 185 VVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKKSG 244
           ++AGK YYG+   D+ EA+  REL+ E     GA +  D++PIL WI   G E  +KK  
Sbjct: 189 MLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI--TGSEKRIKKIA 248

Query: 245 ERADRFMQALIEERRNLKLLGTEKQRN-LLDRLLELQLSEPEYYSDHTIKGIVLVLLRAG 304
            R D F+Q L++ERR     G EK++N ++D LL LQ ++PEYY+D+ IKGI+L L+ AG
Sbjct: 249 SRLDEFLQGLVDERRE----GKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAG 308

Query: 305 TDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFRL 364
           TDTS+VTL W ++ LLN+P++L+KAR EID K+G +R + E DL +L YLQ I+SE+ RL
Sbjct: 309 TDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRL 368

Query: 365 HPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGNE 424
           +PA+P+L+ H +S+DC V GY +PRGT LL NAWAIHRDPK+WDDPTSF PERF   G  
Sbjct: 369 YPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEG-- 428

Query: 425 LNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAE-GGGITIHK 484
             + K++ FG+GRRACPG+ +AQR   LT+G LIQC+EW++ GEE VDM E GGG+ + K
Sbjct: 429 -EAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPK 488

Query: 485 VKPLETMCKARPIMVRVLS 496
             PL  MCKARP++ ++L+
Sbjct: 489 AIPLVAMCKARPVVGKILN 497

BLAST of Sed0020417 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 514.2 bits (1323), Expect = 1.2e-145
Identity = 266/498 (53.41%), Postives = 346/498 (69.48%), Query Frame = 0

Query: 4   LTLLFLLLVAFFILQQLKTRRK-NLPPSPPC-VPVIGHLHLLKRPIHRNFQTIAAKYG-- 63
           L L FL L     L   K+ RK NLPPSP   +PVIGHLHLLK+P+HR F +I+   G  
Sbjct: 7   LLLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNA 66

Query: 64  PIFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYW 123
           PIF LRLG+RL  V+SS  IAE+CFT NDVV ANRP +++++++GYN T +  A YGD+W
Sbjct: 67  PIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHW 126

Query: 124 RNLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSE---WFAKAELKSMFSELSFNVV 183
           RNLRR++AVEIFS+ +++    IRK+EIRR +  L  +    F + ELKS+ + L+FN +
Sbjct: 127 RNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNI 186

Query: 184 MRVVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKK 243
           + +VAGKRYYG    D  EA+  REL+ EI A  G+ +  D++P + W+    FEN  K 
Sbjct: 187 IMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKI 246

Query: 244 SGERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRA 303
            G R DR +Q L++E+R  K    EK + L+D LL  Q +EPEYY+D  IKGI+L L+ A
Sbjct: 247 LGNRLDRVLQKLVDEKRAEK----EKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLA 306

Query: 304 GTDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFR 363
           GTDTSSVTL WAM+ LLN+PE+L KARAEID KIG DR + E D+ NL YLQ I+SET R
Sbjct: 307 GTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLR 366

Query: 364 LHPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGN 423
           L+PA P+LL H+SSD+C VAGYD+PR T LL N WA+HRDP LW++P  F PERF   G 
Sbjct: 367 LYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKEG- 426

Query: 424 ELNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHK 483
              + K++PFG+GRRACPGA + +R + L LG LIQ +EW++ G E VDM EG GIT+ K
Sbjct: 427 --EARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPK 486

Query: 484 VKPLETMCKARPIMVRVL 495
             PL  MCKAR I+ + +
Sbjct: 487 ATPLRAMCKARAIVGKTI 495

BLAST of Sed0020417 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 511.1 bits (1315), Expect = 9.7e-145
Identity = 262/501 (52.30%), Postives = 351/501 (70.06%), Query Frame = 0

Query: 1   MDFLTLLFLLLVAFFILQQLKTRRKNLPPSPP-CVPVIGHLHLLKRPIHRNFQTIAAKYG 60
           M   T  F++L   F++ ++K R+ NLPPSP   +PVIGHL LLK P+HR F +++   G
Sbjct: 5   MLIFTFCFIVLSLIFLIGRIK-RKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLG 64

Query: 61  --PIFTLRLGSRLALVVSSLQIAEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGD 120
             PI +LRLG+RL  VVSS  IAE+CFT NDV+ ANR   + ++++ Y  +T+ +A Y +
Sbjct: 65  DAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSE 124

Query: 121 YWRNLRRLSAVEIFSTAKLNASLCIRKEEIRRF---LLKLHSEWFAKAELKSMFSELSFN 180
           +WRNLRR+ A+EIFS  +LN+   IR++EIRR    LL+  S  F K E+KSMFS+L+FN
Sbjct: 125 HWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFN 184

Query: 181 VVMRVVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSL 240
            ++R++AGK YYG+   D+ EA+  R L+ E  +  G  +  D++PIL WI     E  +
Sbjct: 185 NIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRI 244

Query: 241 KKSGERADRFMQALIEERRNLKLLGTEKQRN-LLDRLLELQLSEPEYYSDHTIKGIVLVL 300
           KK   R D F+Q L++E+R     G EK+ N ++D LL LQ ++PEYY D  IKG +L L
Sbjct: 245 KKLAGRLDEFLQGLVDEKRE----GKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSL 304

Query: 301 LRAGTDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISE 360
           +  GTDT++VTL WA++ LLNNPE+L KAR EID  IG DR + E D+PNL YLQ I+SE
Sbjct: 305 IAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSE 364

Query: 361 TFRLHPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFG 420
           T RL+PAAPMLL H +S DC V GYD+PRGT LL NAWAIHRDP LWDDPTSF PERF  
Sbjct: 365 TLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEK 424

Query: 421 PGNELNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGIT 480
            G    + K++PFG+GRRACPG+ +AQR + L+LG LIQC+EW++ GEE VDM EG G+T
Sbjct: 425 EG---EAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLT 484

Query: 481 IHKVKPLETMCKARPIMVRVL 495
           + K +PLE MC+AR  + ++L
Sbjct: 485 MPKARPLEAMCRARDFVGKIL 495

BLAST of Sed0020417 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 502.7 bits (1293), Expect = 3.5e-142
Identity = 249/499 (49.90%), Postives = 348/499 (69.74%), Query Frame = 0

Query: 7   LFLLLVAFFILQQLKTRRKNLPPSPP-CVPVIGHLHLLKRPIHRNFQTIAAKY-----GP 66
           +F L+      + LK +++NLPPSPP  +P+IGHL LLK PIHR  ++ +        G 
Sbjct: 13  IFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGG 72

Query: 67  IFTLRLGSRLALVVSSLQI-AEQCFTTNDVVFANRPRLLISRYLGYNCTTISTAPYGDYW 126
           + +LRLGSRL  VVSS ++ AE+CF  NDVV ANRP+++I +++GYN T +  APYGD+W
Sbjct: 73  VMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHW 132

Query: 127 RNLRRLSAVEIFSTAKLNASLCIRKEEIRRFLLKLHSEWFAK---AELKSMFSELSFNVV 186
           RNLRRL  +EIFST +LN  L +R +E+RR + +L      K    ELK M  +L+FN +
Sbjct: 133 RNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNI 192

Query: 187 MRVVAGKRYYGEEVSDEAEAREFRELMEEISAQGGASHWEDFMPILKWIGRGGFENSLKK 246
           MR++ GKRYYGEE +DE EA+  R+L+ ++ A   + +  D++PIL+      +EN +KK
Sbjct: 193 MRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENRVKK 252

Query: 247 SGERADRFMQALIEERRNLKLLGTEKQRNLLDRLLELQLSEPEYYSDHTIKGIVLVLLRA 306
            GE  D+F+Q LI+++R  +  GT     ++D LL LQ S+ EYY+D  IKGI+L+++ A
Sbjct: 253 LGEETDKFLQGLIDDKRGQQETGT----TMIDHLLVLQKSDIEYYTDQIIKGIILIMVIA 312

Query: 307 GTDTSSVTLNWAMTQLLNNPEMLAKARAEIDTKIGQDRPINEGDLPNLDYLQAIISETFR 366
           GT+TS+VTL WA++ LLN+P++++KAR EID ++G DR I E DL  L YL+ I+ ET R
Sbjct: 313 GTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLR 372

Query: 367 LHPAAPMLLTHYSSDDCVVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFNPERFFGPGN 426
           LHPA P+L+ H +S+DC +  YD+PRGTTLLVNAWAIHRDP  WDDP SF PERF     
Sbjct: 373 LHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERF---EK 432

Query: 427 ELNSNKIIPFGIGRRACPGASMAQRFIGLTLGLLIQCYEWKKDGEENVDMAEGGGITIHK 486
           E  + K++ FG+GRRACPG+ +AQR +GL LG LIQC+EW++ G   VDM EG G T+ K
Sbjct: 433 EEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPK 492

Query: 487 VKPLETMCKARPIMVRVLS 496
             PL+ +CKARP + +++S
Sbjct: 493 AIPLKAICKARPFLHKIIS 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AEM42992.13.8e-22375.50cytochrome P450 [Siraitia grosvenorii][more]
XP_023512643.16.4e-22376.75cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo][more]
XP_022944057.11.4e-22276.95cytochrome P450 81E8-like [Cucurbita moschata] >KAG7011030.1 Cytochrome P450 81E... [more]
XP_022985607.11.9e-22276.55cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E... [more]
XP_008455812.18.7e-22075.55PREDICTED: cytochrome P450 81E8-like [Cucumis melo] >KAA0032504.1 cytochrome P45... [more]
Match NameE-valueIdentityDescription
W8JMU71.5e-15354.76Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q6WNQ82.0e-14751.63Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
Q9FG654.9e-14149.90Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
Q6WNQ95.4e-14048.69Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
Q9LHA14.6e-13950.30Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
K7NC011.8e-22375.50Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1[more]
A0A6J1FUQ96.9e-22376.95cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=... [more]
A0A6J1J5B59.0e-22376.55cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1[more]
A0A5A7SMV74.2e-22075.55Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A1S3C1R24.2e-22075.55cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103495911 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G37370.11.2e-15053.20cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37340.11.4e-14653.51cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
AT4G37320.11.2e-14553.41cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
AT4G37360.19.7e-14552.30cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT5G36220.13.5e-14249.90cytochrome p450 81d1 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 79..100
score: 21.02
coord: 171..189
score: 22.88
coord: 433..456
score: 28.2
coord: 55..74
score: 39.59
coord: 423..433
score: 54.06
coord: 285..302
score: 36.73
coord: 348..366
score: 35.99
coord: 389..413
score: 39.65
coord: 305..331
score: 37.65
IPR001128Cytochrome P450PRINTSPR00385P450coord: 424..433
score: 58.56
coord: 296..313
score: 39.98
coord: 349..360
score: 41.38
IPR001128Cytochrome P450PFAMPF00067p450coord: 28..476
e-value: 2.7E-94
score: 316.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 14..488
e-value: 1.8E-122
score: 411.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 28..487
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 7..454
e-value: 2.6E-19
score: 67.3
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 4..490
NoneNo IPR availablePANTHERPTHR24298:SF836CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATEDcoord: 4..490
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 426..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0020417.1Sed0020417.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen