Sed0020013 (gene) Chayote v1

BLAST of Sed0020013 vs. NCBI nr
Match: XP_038892956.1 (subtilisin-like protease SBT4.14 [Benincasa hispida])

HSP 1 Score: 1145.6 bits (2962), Expect = 0.0e+00
Identity = 574/743 (77.25%), Postives = 649/743 (87.35%), Query Frame = 0

Query: 1   MSIPKSSCLIVFVLFFIVACFAAVEINEK--HFIVFLENNPVLNEVDAAETHLNVLMSVK 60
           MSI KSS L+VF LFFIV C AA++++++  HFIVFLEN  VLNEVD  ETHLNVLMSVK
Sbjct: 1   MSISKSSRLLVFALFFIVVCVAALDVDKEKNHFIVFLENKSVLNEVDGVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFLG 120
           +SH EAKESMVYSYS++FNAFAAKL+E+EAK LS REDVH+VI NKYR+L TTRSWDFLG
Sbjct: 61  KSHAEAKESMVYSYSKSFNAFAAKLTEDEAKKLSTREDVHHVIPNKYRKLQTTRSWDFLG 120

Query: 121 FPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKL 180
             SNA RNTK ESDI+VGLFDTGI PTADSFKDDGFGPPP+KWKG+C H+ANFTGCN KL
Sbjct: 121 LSSNARRNTKHESDIVVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHYANFTGCNNKL 180

Query: 181 IGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSAR 240
           IGARYFKLD  PDP DI+SP+D +GHGTHTSSTA GN ++GA+L GLA GTARGGVPSAR
Sbjct: 181 IGARYFKLDGSPDPADILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKS 300
           V+MYKVCW S+GCSDMDILAAFDAAI DGVDVIS+SI G GF NYSED ISIGAF AMK 
Sbjct: 241 VSMYKVCWASSGCSDMDILAAFDAAIHDGVDVISISIGGGGFNNYSEDSISIGAFHAMKK 300

Query: 301 GIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNLQ 360
           GIITVT+AGN+GP AGSV+NHAPWIVTVAAS+IDR FISPLELGNG NISGVGIN+ N +
Sbjct: 301 GIITVTSAGNSGPLAGSVVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 QKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGASG 420
           QKMYPLVSGGD ARN ES D AS+C+E S+DP KVK  +V CK+M  GADS++KSLGA+G
Sbjct: 361 QKMYPLVSGGDVARNSESKDSASYCEEDSLDPNKVKGNLVFCKLMTWGADSVIKSLGANG 420

Query: 421 AILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIVASF 480
            I+QSDQFLD  +I+MAPA+MVSS +G  I  YIKSTRTPTA+IYK++QL+A AP+VASF
Sbjct: 421 VIIQSDQFLDNADIFMAPATMVSSSLGTIIHTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHAA 540
           SSRGPN GS RILKPDIAAPGV+ILA+YTPLKSLTGLKGDTQ+SKFTLMSGTSMACPH A
Sbjct: 481 SSRGPNPGSNRILKPDIAAPGVDILASYTPLKSLTGLKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLVYDA 600
           AAAAYVKSFHP WSPAAIRSALITTA P SRRLNPD EFAYGAGNLNP+RA +PGL+YD 
Sbjct: 541 AAAAYVKSFHPHWSPAAIRSALITTARPISRRLNPDGEFAYGAGNLNPSRAISPGLIYDV 600

Query: 601 NEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLPTTV 660
           NE+SY+QFLCSEGY GS+I++L GTKSI+CS L+PGQ HDSLNYPTFQLSL +++ PTT 
Sbjct: 601 NEISYVQFLCSEGYTGSSIAVLAGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTRQPTTT 660

Query: 661 VFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSAGKM 720
            F R VTNV  PV V+NATI APPGVEITV P ++SFSRLL+K+SFKVVVKASPL + KM
Sbjct: 661 TFRRRVTNVAHPVSVFNATIKAPPGVEITVTPTTLSFSRLLQKRSFKVVVKASPLPSTKM 720

Query: 721 VSGSLAWFDGRHVVKSPIVVYSP 742
           VSGS+AW   RHVV+SPIVVYSP
Sbjct: 721 VSGSIAWIGARHVVRSPIVVYSP 743

BLAST of Sed0020013 vs. NCBI nr
Match: XP_011654432.1 (subtilisin-like protease SBT4.14 [Cucumis sativus] >KGN49552.1 hypothetical protein Csa_018364 [Cucumis sativus])

HSP 1 Score: 1137.1 bits (2940), Expect = 0.0e+00
Identity = 571/743 (76.85%), Postives = 648/743 (87.21%), Query Frame = 0

Query: 1   MSIPKSSCLIVFVLFFIVACFAAVEINEK--HFIVFLENNPVLNEVDAAETHLNVLMSVK 60
           MSI KSS L+VF LF +V C A ++ +E+  H+IVFLEN PVLNEVD  ETHLN+LMSVK
Sbjct: 1   MSISKSSRLLVFALFIVVGCVAGLDEDEEKNHYIVFLENKPVLNEVDVVETHLNLLMSVK 60

Query: 61  ESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFLG 120
           +SH EA ESMVYSY+++FNAFAAKLS++EAK LS R+DVH+VI NKYR+L TTRSWDF+G
Sbjct: 61  KSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIG 120

Query: 121 FPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKL 180
             SNA R+TK ESDIIVGLFDTGI PTADSFKDDGFGPPP+KWKG+C HFANFT CNKKL
Sbjct: 121 LSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKL 180

Query: 181 IGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSAR 240
           IGARYFKLD  PDP DI+SP+D +GHGTHTSSTA GN I+GA+L GLA GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKS 300
           VAMYKVCW S+GCSDMDILAAFDAAIQDGVDVIS+SI G GF NYS+D ISIGAF AMK 
Sbjct: 241 VAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKK 300

Query: 301 GIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNLQ 360
           GIITVT+AGN GP+AGSV+NHAPWIVTVAASSIDR FISPLELGNG NISGVGIN+ N +
Sbjct: 301 GIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 QKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGASG 420
           QKMYPLVSGGD ARN ES D ASFC EG++DPTKVK  +V CK++  GADS++KS+GA+G
Sbjct: 361 QKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANG 420

Query: 421 AILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIVASF 480
            I+QSD+FLD  +I+MAPA+MVSS VG  I  YIKSTRTPTA+IYK++QL+A AP+VASF
Sbjct: 421 VIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHAA 540
           SSRGPN GS RILKPDIAAPGV+ILAAYTPLKSLTG KGDTQ+SKFTLMSGTSMACPH A
Sbjct: 481 SSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLVYDA 600
           AAAAYVKSFHPLWSPAAIRSAL+TTATP SRRLNP+ EFAYGAGNLNP+RA +PGL+YD 
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDL 600

Query: 601 NEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLPTTV 660
           NE+SYIQFLCSEGY GS+I++L+GTKSI+CS L+PGQ HDSLNYPTFQLSL ++  P T 
Sbjct: 601 NEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTT 660

Query: 661 VFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSAGKM 720
            F R VTNVG P+ VYNATI APPGV ITV P ++SFSRLL+K+SFKVVVKASPL + KM
Sbjct: 661 TFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKM 720

Query: 721 VSGSLAWFDGRHVVKSPIVVYSP 742
           VSGSLAW   +HVV+SPIVVYSP
Sbjct: 721 VSGSLAWVGAQHVVRSPIVVYSP 743

BLAST of Sed0020013 vs. NCBI nr
Match: XP_038891596.1 (subtilisin-like protease SBT4.14 [Benincasa hispida])

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 565/720 (78.47%), Postives = 641/720 (89.03%), Query Frame = 0

Query: 23   AVEINEKHFIVFLENNP-VLNEVDAAETHLNVLMSVKESHVEAKESMVYSYSQNFNAFAA 82
            ++  + KHFIVFL++ P +LNEVDA E++LNVLMSVKES+VEAK+SMVYSY+ NFNAFAA
Sbjct: 375  SILFSSKHFIVFLKDRPAILNEVDAIESNLNVLMSVKESNVEAKKSMVYSYTNNFNAFAA 434

Query: 83   KLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFLGFPSNATRNTKQESDIIVGLFDTG 142
            KL+EEEAK LS+R DV++VI NKYR+L TTRSWDFLG  SNA RNTK ESDI+VGLFDTG
Sbjct: 435  KLNEEEAKRLSERGDVNHVIPNKYRKLQTTRSWDFLGLSSNARRNTKHESDIVVGLFDTG 494

Query: 143  IAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKLIGARYFKLDDIPDPLDIISPIDV 202
            I PTADSFKDDGFGPPP+KWKG+CLHFANFTGCN KLIGARYFKLD +PDPLDI+SP+DV
Sbjct: 495  ITPTADSFKDDGFGPPPKKWKGTCLHFANFTGCNNKLIGARYFKLDGLPDPLDILSPVDV 554

Query: 203  NGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSARVAMYKVCWVSTGCSDMDILAAFD 262
            NGHGTHT+STAAGN I+GANL GLA GTARGGVPSARVAMYKVCW STGCSDMD+LAAFD
Sbjct: 555  NGHGTHTASTAAGNVIAGANLSGLAQGTARGGVPSARVAMYKVCWTSTGCSDMDLLAAFD 614

Query: 263  AAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKSGIITVTAAGNAGPSAGSVINHAP 322
            AAI+DGVDVIS+SIAG GF NY+EDPISIGAF+AMK GIITV AAGN GPSAG+V+NHAP
Sbjct: 615  AAIRDGVDVISISIAGIGFGNYTEDPISIGAFQAMKKGIITVAAAGNNGPSAGTVVNHAP 674

Query: 323  WIVTVAASSIDRNFISPLELGNGNNISGVGINLLNLQQKMYPLVSGGDAARNQESVDGAS 382
            WIVTVAASSIDR FISPL+LGNG NISGVGINL N QQKMY LVSGGD A++ ES D A 
Sbjct: 675  WIVTVAASSIDRRFISPLQLGNGKNISGVGINLFNPQQKMYKLVSGGDVAKDIESKDSAM 734

Query: 383  FCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGASGAILQSDQFLDVTNIYMAPASMVS 442
            FC EGS+DP KVKD IV CK+M  GADS+VKS+GA GAILQSDQF+D T+I+MAP+++VS
Sbjct: 735  FCVEGSLDPIKVKDSIVFCKLMTWGADSVVKSIGAYGAILQSDQFIDNTDIFMAPSTLVS 794

Query: 443  SFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIVASFSSRGPNSGSKRILKPDIAAPGVN 502
            SFVG +ID+YI STRTPTA+IYK+RQL AAAP VA+FSSRGPN GS RILKPDIAAPGVN
Sbjct: 795  SFVGLTIDSYIHSTRTPTAVIYKTRQLRAAAPFVAAFSSRGPNPGSTRILKPDIAAPGVN 854

Query: 503  ILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHAAAAAAYVKSFHPLWSPAAIRSALI 562
            ILA YTPLKSLTGLKGD+QFSKFTLMSGTSMACPH AAAA YVKSFHPLWSPAAIRSAL+
Sbjct: 855  ILAGYTPLKSLTGLKGDSQFSKFTLMSGTSMACPHVAAAATYVKSFHPLWSPAAIRSALL 914

Query: 563  TTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLVYDANEMSYIQFLCSEGYNGSAISILT 622
            TTATP SRR+NP+ EF YGAGNLNPA+AKNPGL+YD ++MSYIQFLCSEGY GS+I+ILT
Sbjct: 915  TTATPISRRMNPNGEFGYGAGNLNPAKAKNPGLIYDLDDMSYIQFLCSEGYTGSSIAILT 974

Query: 623  GTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLPTTVVFGREVTNVGDPVVVYNATITAP 682
            GTKS+DCST++PGQ +DSLNYPTFQL+L ++Q PTT VF REVTNVG PV  YNATI AP
Sbjct: 975  GTKSLDCSTIIPGQGYDSLNYPTFQLTLKSTQQPTTAVFWREVTNVGRPVSTYNATIRAP 1034

Query: 683  PGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSAGKMVSGSLAWFDGRHVVKSPIVVYSP 742
            PGVEI V P ++SFS+LL+KQ FKVVVK+ PL +  MVSG ++W D R+VV+SPIVVYSP
Sbjct: 1035 PGVEIMVVPTTLSFSQLLQKQRFKVVVKSGPLPSTNMVSGWISWVDVRYVVRSPIVVYSP 1094

BLAST of Sed0020013 vs. NCBI nr
Match: KAE8648139.1 (hypothetical protein Csa_021522 [Cucumis sativus])

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 567/744 (76.21%), Postives = 649/744 (87.23%), Query Frame = 0

Query: 1   MSIPKSSCLIVFVLFFIVACFAAVEI--NEKHFIVFLENNP-VLNEVDAAETHLNVLMSV 60
           MSI K S + + VLF+I  C AAVEI  ++KHFIVF+EN P +LNEVD  +T+LNVLMSV
Sbjct: 1   MSISKFSSVFIIVLFYIAGCVAAVEIVEDKKHFIVFMENRPTILNEVDGLDTNLNVLMSV 60

Query: 61  KESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFL 120
           KESHV+AK+ MV+SY+ NFNAFAAKL+E EAKTLS+R DV +VI N+YR+L TTRSWDFL
Sbjct: 61  KESHVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSWDFL 120

Query: 121 GFPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKK 180
           GFP NA R T+QESDIIVGLFDTGI PTADSFKDDG+GPPP+KWKG+C HFANF+GCN K
Sbjct: 121 GFPINAKRKTRQESDIIVGLFDTGITPTADSFKDDGYGPPPKKWKGTCDHFANFSGCNNK 180

Query: 181 LIGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSA 240
           LIGARYFKLD I +P DI+SP+DVNGHGTHTSSTA GN I+GANL GLA GTA GGVPSA
Sbjct: 181 LIGARYFKLDGITEPFDILSPVDVNGHGTHTSSTATGNVITGANLSGLAQGTAPGGVPSA 240

Query: 241 RVAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMK 300
           R+AMYKVCW+S GCSDMD+LAAFDAAIQDGVDVIS+SIAG G+ NY++DPISIGAF AMK
Sbjct: 241 RLAMYKVCWMSNGCSDMDLLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFHAMK 300

Query: 301 SGIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNL 360
            GIITVTAAGN GPSAG+V+NHAPWI+TVAASSIDR FISP+ELGNG NISGVGINL N 
Sbjct: 301 KGIITVTAAGNNGPSAGTVVNHAPWILTVAASSIDRRFISPVELGNGKNISGVGINLFNP 360

Query: 361 QQKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGAS 420
           ++KMY LVSG D A+N E  D A +C++ S+DP+KVKD +V CK+M  GADS VKS+GA+
Sbjct: 361 EKKMYKLVSGEDVAKNIEGKDNAMYCEDKSLDPSKVKDSLVFCKLMTWGADSTVKSIGAA 420

Query: 421 GAILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIVAS 480
           GAILQSDQFLD T+I+MAP+++VSSFVGA+ID YI STRTPTA+IYK+RQ  AAAPI+A 
Sbjct: 421 GAILQSDQFLDNTDIFMAPSALVSSFVGATIDAYIHSTRTPTAVIYKTRQHRAAAPIIAP 480

Query: 481 FSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHA 540
           FSSRGPN GS  ILKPDIAAPGVNILA YTPLKSLTGLKGDTQFSKFTLMSGTSMACPH 
Sbjct: 481 FSSRGPNPGSTHILKPDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHV 540

Query: 541 AAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLVYD 600
           AAAAAYVKSFHPLWSPAAIRSAL+TTA P SRR NPD EF YGAGNLNP +AKNPGL+YD
Sbjct: 541 AAAAAYVKSFHPLWSPAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPRKAKNPGLIYD 600

Query: 601 ANEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLPTT 660
            NEMSYIQFLC EGY+GS+I ILTGTKSI+C+T++PG+ +DSLNYPTFQLSL +S+ PTT
Sbjct: 601 LNEMSYIQFLCREGYSGSSIIILTGTKSINCATIIPGEGYDSLNYPTFQLSLQSSREPTT 660

Query: 661 VVFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSAGK 720
            VF REVTNVG PV VYNAT+ APPGVEITVEP ++SFS L +K+ FKVVVKA+PL A K
Sbjct: 661 AVFWREVTNVGKPVSVYNATVRAPPGVEITVEPATLSFSYLHQKERFKVVVKANPLPANK 720

Query: 721 MVSGSLAWFDGRHVVKSPIVVYSP 742
           MVSGS+ WFD R+VV+SP+VVYSP
Sbjct: 721 MVSGSITWFDPRYVVRSPVVVYSP 744

BLAST of Sed0020013 vs. NCBI nr
Match: XP_008454753.1 (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis melo] >KAA0056482.1 subtilisin-like protease SBT4.14 [Cucumis melo var. makuwa])

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 570/746 (76.41%), Postives = 640/746 (85.79%), Query Frame = 0

Query: 1   MSIPKSSCLIVFVLFFIVACFA----AVEINEK-HFIVFLENNPVLNEVDAAETHLNVLM 60
           MS+ KSS L+VF LF IV C        E  EK HFIVFL+N PVLNEVDA ETHL+VLM
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SVKESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWD 120
           SVK+SH EA ESMVYSY+++FNAFAAKLS+ EAK LS R+DVH+VI N YR+L TTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FLGFPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCN 180
           F+G  SNA R TK ESDIIVGLFDTGI PTADSF+DDGFGPPP+KWKG+C HFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 KKLIGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVP 240
           KKLIGARYFKLD  PDP DI+SP+D +GHGTHTSSTA GN ++GA+L GLA GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRA 300
           SARVAMYKVCW S+GCSDMDILAAFDAAIQDGVDVIS+SI G GF NYS+D ISIGAF A
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKSGIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLL 360
           MK GIITVT+AGN GP AGSV+NHAPWIVTVAASSIDR FISPLELGNG NISGVGIN+ 
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NLQQKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLG 420
           N ++KMYPLVSGGD ARN ES D ASFC E S+DPTKVK  +V CK++  GADS++KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 ASGAILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIV 480
           A+G I+QSDQFLD  +I+MAPA+MVSS VG  I  YIKSTRTPTA+IYK+RQL+A AP+V
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 ASFSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACP 540
           ASFSSRGPN GS RILKPDIAAPGVNILA YTPLKSLTG KGDTQ+SKFT+MSGTSMACP
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 HAAAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLV 600
           H AAAAAYVKSFHPLWSPAAIRSAL+TT  P SRRLNP+ EFAYGAGNLNP++A +PGL+
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDANEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLP 660
           YD NEMSYIQFLCSEGY GS+I++L GTKSI+CSTL+PG  HDSLNYPTFQLSL +++ P
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 TTVVFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSA 720
            T  F R+VTNVG PV VYNATI APPGV+ITV P ++SFSRLL+K+SFKVVVKASPL +
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 GKMVSGSLAWFDGRHVVKSPIVVYSP 742
            KMVSGSLAW   RHVV+SPIVVYSP
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of Sed0020013 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 7.5e-239
Identity = 425/744 (57.12%), Postives = 554/744 (74.46%), Query Frame = 0

Query: 5   KSSC-----LIVFVLFFIVACFAAVEIN--EKHFIVFLENNPVLNEVDAAETHLNVLMSV 64
           K SC     ++V V+ +I   +A+ E    +  +I++L + P  N  +  +TH+N+L S+
Sbjct: 5   KCSCHHHLLVLVMVVLWISPRYASAEDEHAKDFYIIYLGDRPD-NTEETIKTHINLLSSL 64

Query: 65  KESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFL 124
             S  EAKE  VYSY++ FNAFAAKLS  EAK + + E+V  V +N+YR+L TT+SWDF+
Sbjct: 65  NISQEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFV 124

Query: 125 GFPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKK 184
           G P  A R+ K E D+I+G+ DTGI P ++SF D G GPPP KWKGSC  + NFTGCN K
Sbjct: 125 GLPLTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNK 184

Query: 185 LIGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSA 244
           +IGA+YFK D      ++ SPID++GHGTHTSST AG  ++ A+L G+A GTARG VPSA
Sbjct: 185 IIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSA 244

Query: 245 RVAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMK 304
           R+AMYKVCW  +GC+DMDILA F+AAI DGV++IS+SI G    +YS D IS+G+F AM+
Sbjct: 245 RLAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMR 304

Query: 305 SGIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNL 364
            GI+TV +AGN GPS+G+V NH PWI+TVAAS IDR F S ++LGNG + SG+GI++ + 
Sbjct: 305 KGILTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSP 364

Query: 365 QQKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGAS 424
           + K YPLVSG DAA+N +    A +C   S+D  KVK K+++C++   G +S +KS G +
Sbjct: 365 KAKSYPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGA 424

Query: 425 GAILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIVAS 484
           GAI+ SDQ+LD   I+MAPA+ V+S VG  I  YI STR+ +A+I K+RQ+   AP VAS
Sbjct: 425 GAIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVAS 484

Query: 485 FSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHA 544
           FSSRGPN GS R+LKPDIAAPG++ILAA+T  +SLTGL GDTQFSKFT++SGTSMACPH 
Sbjct: 485 FSSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHV 544

Query: 545 AAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLVYD 604
           A  AAYVKSFHP W+PAAI+SA+IT+A P SRR+N DAEFAYG G +NP RA +PGLVYD
Sbjct: 545 AGVAAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYD 604

Query: 605 ANEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLPTT 664
            +++SY+QFLC EGYN + ++ L GT+S+ CS++VPG  HDSLNYPT QL+L +++  T 
Sbjct: 605 MDDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTL 664

Query: 665 VVFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSAGK 724
            VF R VTNVG P  VY AT+ AP GVEITVEP+S+SFS+  +K+SFKVVVKA  ++ GK
Sbjct: 665 AVFRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGK 724

Query: 725 MVSGSLAWFDGRHVVKSPIVVYSP 742
           +VSG L W   RH V+SPIV+YSP
Sbjct: 725 IVSGLLVWKSPRHSVRSPIVIYSP 746

BLAST of Sed0020013 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 4.5e-175
Identity = 361/755 (47.81%), Postives = 487/755 (64.50%), Query Frame = 0

Query: 11  VFVLFFIV---ACFAAVE---INEKHFIVFLENNPVLNEVDAAETHLNVLMSVKESHVEA 70
           +F+L F +   A  AA E   +  K +IV++      + V+AAE H N+LM+V     +A
Sbjct: 9   LFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTVIGDESKA 68

Query: 71  KESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFLGF-PSNA 130
           +E  +YSY +N N F A+L   EA+ LS+ E V  V +N  R+L TTRSWDFLG   S  
Sbjct: 69  RELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESKY 128

Query: 131 TRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKLIGARY 190
            R+   ES+IIVG+ DTGI   + SF D G GPPP KWKG C+   NFT CN K+IGA+Y
Sbjct: 129 KRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKY 188

Query: 191 FKL--DDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSARVAM 250
           F +  + +PD  +  +  D +GHGTHTSST AG  +S A+L G+A GTARGGVPSAR+A 
Sbjct: 189 FHIQSEGLPDG-EGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAA 248

Query: 251 YKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKSGII 310
           YKVCW  +GC+DMD+LAAFD AI DGVD+IS+SI G   + + EDPI+IGAF AMK GI+
Sbjct: 249 YKVCW-DSGCTDMDMLAAFDEAISDGVDIISISI-GGASLPFFEDPIAIGAFHAMKRGIL 308

Query: 311 TVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNLQQKM 370
           T  +AGN GP   +V N APW++TVAA+S+DR F + ++LGNG   SG+ +N  N ++KM
Sbjct: 309 TTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKM 368

Query: 371 YPLVSGGDAARNQESVDG-ASFCDEGSIDPTKVKDKIVMCKV-------MAKGADSIVKS 430
           YPL SG  A+       G  S C+ G++   KV  K+V C+          +G D +V+S
Sbjct: 369 YPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRS 428

Query: 431 LGASGAILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAP 490
           L  +G I+Q  +  D+    +   S V    G  I  YI ST+ P A+I+K++  +  AP
Sbjct: 429 LKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTKMLAP 488

Query: 491 IVASFSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMA 550
            ++SFS+RGP   S  ILKPDI+APG+NILAAY+ L S+TG   D + + F++MSGTSMA
Sbjct: 489 SISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMA 548

Query: 551 CPHAAAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPG 610
           CPHAAAAAAYVKSFHP WSPAAI+SAL+TTATP   + N +AE +YG+G +NP RA +PG
Sbjct: 549 CPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRRAIHPG 608

Query: 611 LVYDANEMSYIQFLCSEGYNGSAISILTG-------TKSIDCSTLVPGQSHDSLNYPTFQ 670
           LVYD  E +Y++FLC EGYN ++I +LTG        K  +C  +  G   D LNYP+  
Sbjct: 609 LVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLH 668

Query: 671 LSLNNSQLPTTVVFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKV 730
             +N+++   + VF R VTNVG     Y A + AP G+ + V P+ MSF R  EK++FKV
Sbjct: 669 KQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKV 728

Query: 731 VVKASPLSAGK-MVSGSLAWFDGR-HVVKSPIVVY 740
           V+        K +VS S+ W D R H+V+SPI+++
Sbjct: 729 VIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLF 759

BLAST of Sed0020013 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 4.2e-165
Identity = 333/743 (44.82%), Postives = 472/743 (63.53%), Query Frame = 0

Query: 7   SCLIVFVLFFIVACFA--------AVEINEKHFIVFLENNPVLNEVDAAETHLNVLMSVK 66
           S  ++F LFF    F+        + +  +  +IV++     L + D+A  H   ++   
Sbjct: 2   SSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRK--LEDPDSAHLHHRAMLEQV 61

Query: 67  ESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFLG 126
                A ES++++Y ++FN FA KL+EEEA+ ++  E V  V  N+  EL TTRSWDFLG
Sbjct: 62  VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 121

Query: 127 FPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKL 186
           FP    R ++ ES+I+VG+ DTGI P + SF D+GF PPP KWKG+C    NF  CN+K+
Sbjct: 122 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFR-CNRKI 181

Query: 187 IGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSAR 246
           IGAR + +     P D+  P D NGHGTHT+STAAG  +S ANL GL  GTARGGVP AR
Sbjct: 182 IGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLAR 241

Query: 247 VAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKS 306
           +A YKVCW + GCSD DILAA+D AI DGVD+IS+S+ G    +Y  D I+IG+F A++ 
Sbjct: 242 IAAYKVCW-NDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVER 301

Query: 307 GIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNLQ 366
           GI+T  +AGN GP+  +  + +PW+++VAAS++DR F++ +++GNG +  GV IN  + Q
Sbjct: 302 GILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQ 361

Query: 367 QKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGASG 426
              YPLVSG D          + FC + S++P  +K KIV+C+  + G     KSL  + 
Sbjct: 362 --YYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEA-SFGPHEFFKSLDGAA 421

Query: 427 AILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQ-LEAAAPIVAS 486
            +L +    D  + Y  P+S++      +   YI S R+P A I+KS   L A+AP+V S
Sbjct: 422 GVLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVS 481

Query: 487 FSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHA 546
           FSSRGPN  +K ++KPDI+ PGV ILAA+  +  + G++ +T F+   ++SGTSM+CPH 
Sbjct: 482 FSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFN---IISGTSMSCPHI 541

Query: 547 AAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLVYD 606
              A YVK+++P WSPAAI+SAL+TTA+P + R NP AEFAYG+G++NP +A  PGLVYD
Sbjct: 542 TGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYD 601

Query: 607 ANEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLPTT 666
           ANE  Y++FLC +GYN  A+  +TG  S  C++   G+  D LNYP+F LS++ SQ    
Sbjct: 602 ANESDYVKFLCGQGYNTQAVRRITGDYSA-CTSGNTGRVWD-LNYPSFGLSVSPSQ-TFN 661

Query: 667 VVFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSAGK 726
             F R +T+V      Y A I+AP G+ I+V P  +SF+ L +++SF + V+ S    G 
Sbjct: 662 QYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGS--IKGF 721

Query: 727 MVSGSLAWFDGRHVVKSPIVVYS 741
           +VS SL W DG H V+SPI + S
Sbjct: 722 VVSASLVWSDGVHYVRSPITITS 729

BLAST of Sed0020013 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 4.2e-157
Identity = 319/715 (44.62%), Postives = 447/715 (62.52%), Query Frame = 0

Query: 31  FIVFLENNPVLNEVDAAETHLNVLMSVKESHVEAKESMVYSYSQNFNAFAAKLSEEEAKT 90
           +IV++   P + +      HL++L  +  + + A   +V SY ++FN FAA LS+ E++ 
Sbjct: 33  YIVYMGTLPEI-KYSPPSHHLSILQKLVGT-IAASHLLVRSYKRSFNGFAANLSQAESQK 92

Query: 91  LSKREDVHYVIQNKYRELLTTRSWDFLGFPSNATRNTKQESDIIVGLFDTGIAPTADSFK 150
           L   ++V  V  +K  EL TTRSWDF+GF   A R + +ESD+IVG+ D+GI P ++SF 
Sbjct: 93  LQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFD 152

Query: 151 DDGFGPPPQKWKGSCLHFANFTGCNKKLIGARYF-KLDDIPDPLDIISPIDVNGHGTHTS 210
           D+GFGPPP+KWKGSC     F  CN KLIGAR++ K  D        S  D  GHGTHT+
Sbjct: 153 DEGFGPPPKKWKGSCKGGLKF-ACNNKLIGARFYNKFAD--------SARDEEGHGTHTA 212

Query: 211 STAAGNEISGANLGGLATGTARGGVPSARVAMYKVCWVSTGCSDMDILAAFDAAIQDGVD 270
           STAAGN +  A+  GLA GTARGGVPSAR+A YKVC+    C+D+DILAAFD AI DGVD
Sbjct: 213 STAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF--NRCNDVDILAAFDDAIADGVD 272

Query: 271 VISVSIAGNGFVNYSEDPISIGAFRAMKSGIITVTAAGNAGPSAGSVINHAPWIVTVAAS 330
           VIS+SI+ +   N     ++IG+F AM  GIIT  +AGN GP  GSV N +PW++TVAAS
Sbjct: 273 VISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAAS 332

Query: 331 SIDRNFISPLELGNGNNISGVGINLLNLQQKMYPLVSGGDAARNQESVDGASFCDEGSID 390
             DR FI  + LGNG  ++G+ +N  NL    +P+V G + +RN      A +C  G +D
Sbjct: 333 GTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQ-AGYCSSGCVD 392

Query: 391 PTKVKDKIVMCKVMAKGADSIVKSLGASGAILQSDQFLDVTNIYMAPASMVSSFVGASID 450
              VK KIV+C       ++ +   GA G I+Q+    D   +   PAS +      SI 
Sbjct: 393 SELVKGKIVLCDDFLGYREAYL--AGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIK 452

Query: 451 NYIKSTRTPTAMIYKSRQL-EAAAPIVASFSSRGPNSGSKRILKPDIAAPGVNILAAYTP 510
           +YI+S   P A I ++ ++ +  AP V SFSSRGP+   + +LKPD++APG+ ILAA++P
Sbjct: 453 SYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSP 512

Query: 511 LKSLTGLKG--DTQFSKFTLMSGTSMACPHAAAAAAYVKSFHPLWSPAAIRSALITTATP 570
           + S +      D +  ++++MSGTSMACPH A  AAYVKSFHP WSP+AI+SA++TTATP
Sbjct: 513 VASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATP 572

Query: 571 SSRRLNPDAEFAYGAGNLNPARAKNPGLVYDANEMSYIQFLCSEGYNGSAISILTGTKSI 630
            + + NP+ EFAYG+G +NP +A +PGLVY+     Y++ LC+EG++ + ++  +G +++
Sbjct: 573 MNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG-QNV 632

Query: 631 DCSTLVPGQSHDSLNYPTFQLSLNNSQLPTTVVFGREVTNVGDPVVVYNATIT-APPGVE 690
            CS     +    LNYPT    +++   P  V F R VTNVG P   Y A++    P ++
Sbjct: 633 TCSERTEVK---DLNYPTMTTFVSSLD-PFNVTFKRTVTNVGFPNSTYKASVVPLQPELQ 692

Query: 691 ITVEPRSMSFSRLLEKQSFKVVVKASPLSAGKMVSGSLAWFDGRHVVKSPIVVYS 741
           I++EP  + F  L EK+SF V +    L  G  VS S+ W DG H V+SPIV YS
Sbjct: 693 ISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 726

BLAST of Sed0020013 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 5.5e-149
Identity = 315/750 (42.00%), Postives = 459/750 (61.20%), Query Frame = 0

Query: 2   SIPKSSCLIVFVLFFIVACFAAVEINEKHFIVFLENNPVLNEVDAAETHLNVLMSVK-ES 61
           ++  SS L+  +L   ++  +AV  +++ +IV++ +     +      H+N+L  V  ES
Sbjct: 3   TLAASSSLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGES 62

Query: 62  HVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFLGFP 121
            +E +  +V SY ++FN FAA+L+E E + ++K   V  V  NK  +L TT SWDF+G  
Sbjct: 63  SIEGR--LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 122

Query: 122 S--NATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKL 181
                 RN   ESD I+G+ D+GI P + SF D GFGPPPQKWKG C    NFT CN KL
Sbjct: 123 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT-CNNKL 182

Query: 182 IGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSAR 241
           IGAR +  +            D++GHGTHT+STAAGN +  A+  G+  GT RGGVP++R
Sbjct: 183 IGARDYTSE---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASR 242

Query: 242 VAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKS 301
           VA YKVC   TGCS   +L+AFD AI DGVD+I++SI       +  DPI+IGAF AM  
Sbjct: 243 VAAYKVC-TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAK 302

Query: 302 GIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNLQ 361
           G++TV +AGN+GP   SV   APWI+TVAAS+ +R F++ + LGNG  + G  +N   ++
Sbjct: 303 GVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMK 362

Query: 362 QKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGASG 421
            K YPLV G  AA +    + A  C+   +D ++VK KI++C     G   IV+S+GA G
Sbjct: 363 GKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG--GPGGLKIVESVGAVG 422

Query: 422 AILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQL-EAAAPIVAS 481
            I ++ +  DV  I+  PA+ + +    S+ +Y++ST +P A++ K+  +    +P++AS
Sbjct: 423 LIYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIAS 482

Query: 482 FSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHA 541
           FSSRGPN+ +  ILKPDI APGV ILAAY+P    +  + DT+  K++++SGTSM+CPH 
Sbjct: 483 FSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHV 542

Query: 542 AAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLN--PDAEFAYGAGNLNPARAKNPGLV 601
           A  AAYVK+F+P WSP+ I+SA++TTA P +         EFAYG+G+++P  A NPGLV
Sbjct: 543 AGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLV 602

Query: 602 YDANEMSYIQFLCSEGYNGSAISILTGTKSIDCS---TLVPGQSHDSLNYPTFQLSLNNS 661
           Y+ ++  +I FLC   Y    + +++G +++ CS    ++P     +LNYP+    L+ S
Sbjct: 603 YELDKSDHIAFLCGMNYTSQVLKVISG-ETVTCSEAKKILP----RNLNYPSMSAKLSGS 662

Query: 662 QLPTTVVFGREVTNVGDPVVVYNATITAPPG--VEITVEPRSMSFSRLLEKQSFKVVVKA 721
               TV F R +TNVG P   Y + + A  G  +++ + P  +SF  + EKQSF V V  
Sbjct: 663 GTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTG 722

Query: 722 SPLSAGKMVSGSLAWFDGRHVVKSPIVVYS 741
           S L +    S +L W DG H V+SPIVVY+
Sbjct: 723 SNLDSEVPSSANLIWSDGTHNVRSPIVVYT 729

BLAST of Sed0020013 vs. ExPASy TrEMBL
Match: A0A0A0KME6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G000010 PE=3 SV=1)

HSP 1 Score: 1137.1 bits (2940), Expect = 0.0e+00
Identity = 571/743 (76.85%), Postives = 648/743 (87.21%), Query Frame = 0

Query: 1   MSIPKSSCLIVFVLFFIVACFAAVEINEK--HFIVFLENNPVLNEVDAAETHLNVLMSVK 60
           MSI KSS L+VF LF +V C A ++ +E+  H+IVFLEN PVLNEVD  ETHLN+LMSVK
Sbjct: 1   MSISKSSRLLVFALFIVVGCVAGLDEDEEKNHYIVFLENKPVLNEVDVVETHLNLLMSVK 60

Query: 61  ESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFLG 120
           +SH EA ESMVYSY+++FNAFAAKLS++EAK LS R+DVH+VI NKYR+L TTRSWDF+G
Sbjct: 61  KSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIG 120

Query: 121 FPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKL 180
             SNA R+TK ESDIIVGLFDTGI PTADSFKDDGFGPPP+KWKG+C HFANFT CNKKL
Sbjct: 121 LSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKL 180

Query: 181 IGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSAR 240
           IGARYFKLD  PDP DI+SP+D +GHGTHTSSTA GN I+GA+L GLA GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKS 300
           VAMYKVCW S+GCSDMDILAAFDAAIQDGVDVIS+SI G GF NYS+D ISIGAF AMK 
Sbjct: 241 VAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKK 300

Query: 301 GIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNLQ 360
           GIITVT+AGN GP+AGSV+NHAPWIVTVAASSIDR FISPLELGNG NISGVGIN+ N +
Sbjct: 301 GIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 QKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGASG 420
           QKMYPLVSGGD ARN ES D ASFC EG++DPTKVK  +V CK++  GADS++KS+GA+G
Sbjct: 361 QKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANG 420

Query: 421 AILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIVASF 480
            I+QSD+FLD  +I+MAPA+MVSS VG  I  YIKSTRTPTA+IYK++QL+A AP+VASF
Sbjct: 421 VIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHAA 540
           SSRGPN GS RILKPDIAAPGV+ILAAYTPLKSLTG KGDTQ+SKFTLMSGTSMACPH A
Sbjct: 481 SSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLVYDA 600
           AAAAYVKSFHPLWSPAAIRSAL+TTATP SRRLNP+ EFAYGAGNLNP+RA +PGL+YD 
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDL 600

Query: 601 NEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLPTTV 660
           NE+SYIQFLCSEGY GS+I++L+GTKSI+CS L+PGQ HDSLNYPTFQLSL ++  P T 
Sbjct: 601 NEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTT 660

Query: 661 VFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSAGKM 720
            F R VTNVG P+ VYNATI APPGV ITV P ++SFSRLL+K+SFKVVVKASPL + KM
Sbjct: 661 TFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKM 720

Query: 721 VSGSLAWFDGRHVVKSPIVVYSP 742
           VSGSLAW   +HVV+SPIVVYSP
Sbjct: 721 VSGSLAWVGAQHVVRSPIVVYSP 743

BLAST of Sed0020013 vs. ExPASy TrEMBL
Match: A0A5A7UKY8 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold186G002590 PE=3 SV=1)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 570/746 (76.41%), Postives = 640/746 (85.79%), Query Frame = 0

Query: 1   MSIPKSSCLIVFVLFFIVACFA----AVEINEK-HFIVFLENNPVLNEVDAAETHLNVLM 60
           MS+ KSS L+VF LF IV C        E  EK HFIVFL+N PVLNEVDA ETHL+VLM
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SVKESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWD 120
           SVK+SH EA ESMVYSY+++FNAFAAKLS+ EAK LS R+DVH+VI N YR+L TTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FLGFPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCN 180
           F+G  SNA R TK ESDIIVGLFDTGI PTADSF+DDGFGPPP+KWKG+C HFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 KKLIGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVP 240
           KKLIGARYFKLD  PDP DI+SP+D +GHGTHTSSTA GN ++GA+L GLA GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRA 300
           SARVAMYKVCW S+GCSDMDILAAFDAAIQDGVDVIS+SI G GF NYS+D ISIGAF A
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKSGIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLL 360
           MK GIITVT+AGN GP AGSV+NHAPWIVTVAASSIDR FISPLELGNG NISGVGIN+ 
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NLQQKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLG 420
           N ++KMYPLVSGGD ARN ES D ASFC E S+DPTKVK  +V CK++  GADS++KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 ASGAILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIV 480
           A+G I+QSDQFLD  +I+MAPA+MVSS VG  I  YIKSTRTPTA+IYK+RQL+A AP+V
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 ASFSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACP 540
           ASFSSRGPN GS RILKPDIAAPGVNILA YTPLKSLTG KGDTQ+SKFT+MSGTSMACP
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 HAAAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLV 600
           H AAAAAYVKSFHPLWSPAAIRSAL+TT  P SRRLNP+ EFAYGAGNLNP++A +PGL+
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDANEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLP 660
           YD NEMSYIQFLCSEGY GS+I++L GTKSI+CSTL+PG  HDSLNYPTFQLSL +++ P
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 TTVVFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSA 720
            T  F R+VTNVG PV VYNATI APPGV+ITV P ++SFSRLL+K+SFKVVVKASPL +
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 GKMVSGSLAWFDGRHVVKSPIVVYSP 742
            KMVSGSLAW   RHVV+SPIVVYSP
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of Sed0020013 vs. ExPASy TrEMBL
Match: A0A1S3C036 (subtilisin-like protease SBT4.14 OS=Cucumis melo OX=3656 GN=LOC103495082 PE=3 SV=1)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 570/746 (76.41%), Postives = 640/746 (85.79%), Query Frame = 0

Query: 1   MSIPKSSCLIVFVLFFIVACFA----AVEINEK-HFIVFLENNPVLNEVDAAETHLNVLM 60
           MS+ KSS L+VF LF IV C        E  EK HFIVFL+N PVLNEVDA ETHL+VLM
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SVKESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWD 120
           SVK+SH EA ESMVYSY+++FNAFAAKLS+ EAK LS R+DVH+VI N YR+L TTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FLGFPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCN 180
           F+G  SNA R TK ESDIIVGLFDTGI PTADSF+DDGFGPPP+KWKG+C HFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 KKLIGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVP 240
           KKLIGARYFKLD  PDP DI+SP+D +GHGTHTSSTA GN ++GA+L GLA GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRA 300
           SARVAMYKVCW S+GCSDMDILAAFDAAIQDGVDVIS+SI G GF NYS+D ISIGAF A
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKSGIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLL 360
           MK GIITVT+AGN GP AGSV+NHAPWIVTVAASSIDR FISPLELGNG NISGVGIN+ 
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NLQQKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLG 420
           N ++KMYPLVSGGD ARN ES D ASFC E S+DPTKVK  +V CK++  GADS++KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 ASGAILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIV 480
           A+G I+QSDQFLD  +I+MAPA+MVSS VG  I  YIKSTRTPTA+IYK+RQL+A AP+V
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 ASFSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACP 540
           ASFSSRGPN GS RILKPDIAAPGVNILA YTPLKSLTG KGDTQ+SKFT+MSGTSMACP
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 HAAAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLV 600
           H AAAAAYVKSFHPLWSPAAIRSAL+TT  P SRRLNP+ EFAYGAGNLNP++A +PGL+
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDANEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLP 660
           YD NEMSYIQFLCSEGY GS+I++L GTKSI+CSTL+PG  HDSLNYPTFQLSL +++ P
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 TTVVFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSA 720
            T  F R+VTNVG PV VYNATI APPGV+ITV P ++SFSRLL+K+SFKVVVKASPL +
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 GKMVSGSLAWFDGRHVVKSPIVVYSP 742
            KMVSGSLAW   RHVV+SPIVVYSP
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of Sed0020013 vs. ExPASy TrEMBL
Match: A0A1S3BZ36 (subtilisin-like protease SBT4.14 OS=Cucumis melo OX=3656 GN=LOC103495168 PE=3 SV=1)

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 561/715 (78.46%), Postives = 633/715 (88.53%), Query Frame = 0

Query: 28  EKHFIVFLEN-NPVLNEVDAAETHLNVLMSVKESHVEAKESMVYSYSQNFNAFAAKLSEE 87
           +KHFIVFLEN   +LNEVD  + +LNVLMSVKESHVEAKESMVYSY+ NFNAFAAKL+E 
Sbjct: 61  QKHFIVFLENGRTILNEVDGLDINLNVLMSVKESHVEAKESMVYSYTNNFNAFAAKLTEA 120

Query: 88  EAKTLSKREDVHYVIQNKYRELLTTRSWDFLGFPSNATRNTKQESDIIVGLFDTGIAPTA 147
           EAKTLS+R DV +VI N+YR+L TTRSWDFLGFP NA R T+QESDIIVGLFDTGI PTA
Sbjct: 121 EAKTLSERGDVQHVIPNRYRKLQTTRSWDFLGFPINAKRKTRQESDIIVGLFDTGITPTA 180

Query: 148 DSFKDDGFGPPPQKWKGSCLHFANFTGCNKKLIGARYFKLDDIPDPLDIISPIDVNGHGT 207
           DSFKDDGFGPPP+KWKG+C HFANF+GCN KLIGARYFKLD + +PLDI+SPID+NGHGT
Sbjct: 181 DSFKDDGFGPPPKKWKGTCDHFANFSGCNNKLIGARYFKLDGLTEPLDILSPIDINGHGT 240

Query: 208 HTSSTAAGNEISGANLGGLATGTARGGVPSARVAMYKVCWVSTGCSDMDILAAFDAAIQD 267
           HTSSTA GN I+GANL GLA GTARGGVPSARVAMYKVCW STGCSDMD+LAAFDAAIQD
Sbjct: 241 HTSSTATGNVITGANLSGLAQGTARGGVPSARVAMYKVCWTSTGCSDMDLLAAFDAAIQD 300

Query: 268 GVDVISVSIAGNGFVNYSEDPISIGAFRAMKSGIITVTAAGNAGPSAGSVINHAPWIVTV 327
           GVDVIS+SIAG G+ NY+EDPISIGAF AMK GIITVTAAGN GPSAG+V+NHAPWI+TV
Sbjct: 301 GVDVISISIAGIGYGNYTEDPISIGAFHAMKKGIITVTAAGNNGPSAGTVVNHAPWILTV 360

Query: 328 AASSIDRNFISPLELGNGNNISGVGINLLNLQQKMYPLVSGGDAARNQESVDGASFCDEG 387
           AASSIDR FISP+ELGNG NISGVGINL N ++KMY LVSG D A+N ES D A++C E 
Sbjct: 361 AASSIDRTFISPVELGNGKNISGVGINLFNPEKKMYKLVSGEDVAKNIESKDDATYCVEN 420

Query: 388 SIDPTKVKDKIVMCKVMAKGADSIVKSLGASGAILQSDQFLDVTNIYMAPASMVSSFVGA 447
           S+DP+KVKD IV CK++  GADS VKS+GA+GAILQSDQFLD T+I+MAP+++VSSFVGA
Sbjct: 421 SLDPSKVKDSIVFCKLLTWGADSAVKSIGAAGAILQSDQFLDNTDIFMAPSTLVSSFVGA 480

Query: 448 SIDNYIKSTRTPTAMIYKSRQLEAAAPIVASFSSRGPNSGSKRILKPDIAAPGVNILAAY 507
           +ID+YI S+RTPTA+IYK+RQ   AAPIVA FSSRGPN GS RILKPDIAAPGVNILA Y
Sbjct: 481 TIDSYIHSSRTPTAVIYKTRQHIGAAPIVAPFSSRGPNPGSTRILKPDIAAPGVNILAGY 540

Query: 508 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHAAAAAAYVKSFHPLWSPAAIRSALITTATP 567
           TPLKSLTGLKGDTQFSKFTLMSGTSMACPH AAAAAYVKSFHPLWSPAAIRSAL+TTA P
Sbjct: 541 TPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTAKP 600

Query: 568 SSRRLNPDAEFAYGAGNLNPARAKNPGLVYDANEMSYIQFLCSEGYNGSAISILTGTKSI 627
            SRR NPD EF YGAGNLNP +AKNPGL+YD NEMSYIQFLCSEGY+GS+ISILTGTKSI
Sbjct: 601 ISRRGNPDGEFGYGAGNLNPGKAKNPGLIYDLNEMSYIQFLCSEGYSGSSISILTGTKSI 660

Query: 628 DCSTLVPGQSHDSLNYPTFQLSLNNSQLPTTVVFGREVTNVGDPVVVYNATITAPPGVEI 687
           +C+T++ GQ +DSLNYPTFQL L +++ PTT VF REVTNVG PV VYN T+ APPGVEI
Sbjct: 661 NCATIIRGQGYDSLNYPTFQLRLQSTRQPTTAVFWREVTNVGTPVSVYNTTVWAPPGVEI 720

Query: 688 TVEPRSMSFSRLLEKQSFKVVVKASPLSAGKMVSGSLAWFDGRHVVKSPIVVYSP 742
           TVEP ++SFS LL+K+ FKVVVKA+PL + KMVSGS+ WF+ R+VV+SPIVVYSP
Sbjct: 721 TVEPATLSFSYLLQKERFKVVVKANPLPSNKMVSGSITWFNPRYVVRSPIVVYSP 775

BLAST of Sed0020013 vs. ExPASy TrEMBL
Match: A0A6J1IN55 (subtilisin-like protease SBT4.14 OS=Cucurbita maxima OX=3661 GN=LOC111477941 PE=3 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 556/743 (74.83%), Postives = 633/743 (85.20%), Query Frame = 0

Query: 1   MSIPKSSCLIVFVLFFIVACFAAVEINE--KHFIVFLENNPVLNEVDAAETHLNVLMSVK 60
           M I  SS ++V VL F+V C  AVEI+E  KHFIVFLE  P LNE DA ETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFLG 120
           ES+VEA+ESMVYSY+++FNAFAAKL+EEEA  LSKREDVH+VI N+YR+L TTRSWDF+G
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120

Query: 121 FPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKL 180
             S+A RN+K E+DIIVGLFDTGI PTADSFKDDGFGPPP KWKG+C HFANFTGCNKKL
Sbjct: 121 LSSHAKRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKL 180

Query: 181 IGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSAR 240
           IGARYFKLD   DP DI+SPIDV+GHGTHTSSTA GN ++GA+L GLA GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKS 300
           VAMYKVCW STGC+DMDILAAFDAAI DGVDVIS+SI G  F NYS+D ISIGAF AMK 
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKK 300

Query: 301 GIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNLQ 360
           GIITVT+AGN GP+ GSV+NHAPWIVTV AS+IDR FIS LELGNG NISGVGIN+ N +
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPK 360

Query: 361 QKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGASG 420
           +KMYPLV GGD AR   + + AS C+E S+DP+KVK  +V C+++  G DS+V +LGA+G
Sbjct: 361 KKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANG 420

Query: 421 AILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIVASF 480
           AI+QS+++LD  NI+MAPA+MVSS VGA I +YIKSTRTPTA+IYK+RQL+AAAP+ ASF
Sbjct: 421 AIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHAA 540
           SSRGPN G+ RILKPDIAAPGV+ILA YTPLKSLTG +GDTQFSKFTLMSGTSMACPH A
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLVYDA 600
           AAAAYVKSFHPLWSP AIRSALITTA+  SRRLNPD EFAYGAGNLNP+RA NPGL+YD 
Sbjct: 541 AAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLPTTV 660
           NEMSYIQFLCSEGY G +IS+LTGTKSI+CSTL+PG  HDSLNYPTFQL L + +   + 
Sbjct: 601 NEMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMST 660

Query: 661 VFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSAGKM 720
           VF R VTNV  PV VYNATI APPGVEITV P ++SFSRL +K+SFKV VKASPL++GKM
Sbjct: 661 VFRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKM 720

Query: 721 VSGSLAWFDGRHVVKSPIVVYSP 742
           VSGS+AW   RH V+SPIVVYSP
Sbjct: 721 VSGSVAWIGARHAVRSPIVVYSP 742

BLAST of Sed0020013 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 828.2 bits (2138), Expect = 5.3e-240
Identity = 425/744 (57.12%), Postives = 554/744 (74.46%), Query Frame = 0

Query: 5   KSSC-----LIVFVLFFIVACFAAVEIN--EKHFIVFLENNPVLNEVDAAETHLNVLMSV 64
           K SC     ++V V+ +I   +A+ E    +  +I++L + P  N  +  +TH+N+L S+
Sbjct: 5   KCSCHHHLLVLVMVVLWISPRYASAEDEHAKDFYIIYLGDRPD-NTEETIKTHINLLSSL 64

Query: 65  KESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFL 124
             S  EAKE  VYSY++ FNAFAAKLS  EAK + + E+V  V +N+YR+L TT+SWDF+
Sbjct: 65  NISQEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFV 124

Query: 125 GFPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKK 184
           G P  A R+ K E D+I+G+ DTGI P ++SF D G GPPP KWKGSC  + NFTGCN K
Sbjct: 125 GLPLTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNK 184

Query: 185 LIGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSA 244
           +IGA+YFK D      ++ SPID++GHGTHTSST AG  ++ A+L G+A GTARG VPSA
Sbjct: 185 IIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSA 244

Query: 245 RVAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMK 304
           R+AMYKVCW  +GC+DMDILA F+AAI DGV++IS+SI G    +YS D IS+G+F AM+
Sbjct: 245 RLAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMR 304

Query: 305 SGIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNL 364
            GI+TV +AGN GPS+G+V NH PWI+TVAAS IDR F S ++LGNG + SG+GI++ + 
Sbjct: 305 KGILTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSP 364

Query: 365 QQKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGAS 424
           + K YPLVSG DAA+N +    A +C   S+D  KVK K+++C++   G +S +KS G +
Sbjct: 365 KAKSYPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGA 424

Query: 425 GAILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAPIVAS 484
           GAI+ SDQ+LD   I+MAPA+ V+S VG  I  YI STR+ +A+I K+RQ+   AP VAS
Sbjct: 425 GAIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVAS 484

Query: 485 FSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHA 544
           FSSRGPN GS R+LKPDIAAPG++ILAA+T  +SLTGL GDTQFSKFT++SGTSMACPH 
Sbjct: 485 FSSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHV 544

Query: 545 AAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPGLVYD 604
           A  AAYVKSFHP W+PAAI+SA+IT+A P SRR+N DAEFAYG G +NP RA +PGLVYD
Sbjct: 545 AGVAAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYD 604

Query: 605 ANEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLPTT 664
            +++SY+QFLC EGYN + ++ L GT+S+ CS++VPG  HDSLNYPT QL+L +++  T 
Sbjct: 605 MDDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTL 664

Query: 665 VVFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKVVVKASPLSAGK 724
            VF R VTNVG P  VY AT+ AP GVEITVEP+S+SFS+  +K+SFKVVVKA  ++ GK
Sbjct: 665 AVFRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGK 724

Query: 725 MVSGSLAWFDGRHVVKSPIVVYSP 742
           +VSG L W   RH V+SPIV+YSP
Sbjct: 725 IVSGLLVWKSPRHSVRSPIVIYSP 746

BLAST of Sed0020013 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 616.3 bits (1588), Expect = 3.2e-176
Identity = 361/755 (47.81%), Postives = 487/755 (64.50%), Query Frame = 0

Query: 11  VFVLFFIV---ACFAAVE---INEKHFIVFLENNPVLNEVDAAETHLNVLMSVKESHVEA 70
           +F+L F +   A  AA E   +  K +IV++      + V+AAE H N+LM+V     +A
Sbjct: 9   LFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTVIGDESKA 68

Query: 71  KESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFLGF-PSNA 130
           +E  +YSY +N N F A+L   EA+ LS+ E V  V +N  R+L TTRSWDFLG   S  
Sbjct: 69  RELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESKY 128

Query: 131 TRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKLIGARY 190
            R+   ES+IIVG+ DTGI   + SF D G GPPP KWKG C+   NFT CN K+IGA+Y
Sbjct: 129 KRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKY 188

Query: 191 FKL--DDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSARVAM 250
           F +  + +PD  +  +  D +GHGTHTSST AG  +S A+L G+A GTARGGVPSAR+A 
Sbjct: 189 FHIQSEGLPDG-EGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAA 248

Query: 251 YKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKSGII 310
           YKVCW  +GC+DMD+LAAFD AI DGVD+IS+SI G   + + EDPI+IGAF AMK GI+
Sbjct: 249 YKVCW-DSGCTDMDMLAAFDEAISDGVDIISISI-GGASLPFFEDPIAIGAFHAMKRGIL 308

Query: 311 TVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNLQQKM 370
           T  +AGN GP   +V N APW++TVAA+S+DR F + ++LGNG   SG+ +N  N ++KM
Sbjct: 309 TTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKM 368

Query: 371 YPLVSGGDAARNQESVDG-ASFCDEGSIDPTKVKDKIVMCKV-------MAKGADSIVKS 430
           YPL SG  A+       G  S C+ G++   KV  K+V C+          +G D +V+S
Sbjct: 369 YPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRS 428

Query: 431 LGASGAILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQLEAAAP 490
           L  +G I+Q  +  D+    +   S V    G  I  YI ST+ P A+I+K++  +  AP
Sbjct: 429 LKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTKMLAP 488

Query: 491 IVASFSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMA 550
            ++SFS+RGP   S  ILKPDI+APG+NILAAY+ L S+TG   D + + F++MSGTSMA
Sbjct: 489 SISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMA 548

Query: 551 CPHAAAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLNPARAKNPG 610
           CPHAAAAAAYVKSFHP WSPAAI+SAL+TTATP   + N +AE +YG+G +NP RA +PG
Sbjct: 549 CPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRRAIHPG 608

Query: 611 LVYDANEMSYIQFLCSEGYNGSAISILTG-------TKSIDCSTLVPGQSHDSLNYPTFQ 670
           LVYD  E +Y++FLC EGYN ++I +LTG        K  +C  +  G   D LNYP+  
Sbjct: 609 LVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLH 668

Query: 671 LSLNNSQLPTTVVFGREVTNVGDPVVVYNATITAPPGVEITVEPRSMSFSRLLEKQSFKV 730
             +N+++   + VF R VTNVG     Y A + AP G+ + V P+ MSF R  EK++FKV
Sbjct: 669 KQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKV 728

Query: 731 VVKASPLSAGK-MVSGSLAWFDGR-HVVKSPIVVY 740
           V+        K +VS S+ W D R H+V+SPI+++
Sbjct: 729 VIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLF 759

BLAST of Sed0020013 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 557.0 bits (1434), Expect = 2.3e-158
Identity = 316/696 (45.40%), Postives = 439/696 (63.07%), Query Frame = 0

Query: 50  HLNVLMSVKESHVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELL 109
           HL++L  +  + + A   +V SY ++FN FAA LS+ E++ L   ++V  V  +K  EL 
Sbjct: 15  HLSILQKLVGT-IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELT 74

Query: 110 TTRSWDFLGFPSNATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFA 169
           TTRSWDF+GF   A R + +ESD+IVG+ D+GI P ++SF D+GFGPPP+KWKGSC    
Sbjct: 75  TTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGL 134

Query: 170 NFTGCNKKLIGARYF-KLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATG 229
            F  CN KLIGAR++ K  D        S  D  GHGTHT+STAAGN +  A+  GLA G
Sbjct: 135 KF-ACNNKLIGARFYNKFAD--------SARDEEGHGTHTASTAAGNAVQAASFYGLAQG 194

Query: 230 TARGGVPSARVAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPI 289
           TARGGVPSAR+A YKVC+    C+D+DILAAFD AI DGVDVIS+SI+ +   N     +
Sbjct: 195 TARGGVPSARIAAYKVCF--NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASV 254

Query: 290 SIGAFRAMKSGIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNIS 349
           +IG+F AM  GIIT  +AGN GP  GSV N +PW++TVAAS  DR FI  + LGNG  ++
Sbjct: 255 AIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALT 314

Query: 350 GVGINLLNLQQKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGAD 409
           G+ +N  NL    +P+V G + +RN      A +C  G +D   VK KIV+C       +
Sbjct: 315 GISVNTFNLNGTKFPIVYGQNVSRNCSQAQ-AGYCSSGCVDSELVKGKIVLCDDFLGYRE 374

Query: 410 SIVKSLGASGAILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQL 469
           + +   GA G I+Q+    D   +   PAS +      SI +YI+S   P A I ++ ++
Sbjct: 375 AYL--AGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEI 434

Query: 470 -EAAAPIVASFSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKG--DTQFSKFT 529
            +  AP V SFSSRGP+   + +LKPD++APG+ ILAA++P+ S +      D +  +++
Sbjct: 435 VDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYS 494

Query: 530 LMSGTSMACPHAAAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLNPDAEFAYGAGNLN 589
           +MSGTSMACPH A  AAYVKSFHP WSP+AI+SA++TTATP + + NP+ EFAYG+G +N
Sbjct: 495 VMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQIN 554

Query: 590 PARAKNPGLVYDANEMSYIQFLCSEGYNGSAISILTGTKSIDCSTLVPGQSHDSLNYPTF 649
           P +A +PGLVY+     Y++ LC+EG++ + ++  +G +++ CS     +    LNYPT 
Sbjct: 555 PTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG-QNVTCSERTEVK---DLNYPTM 614

Query: 650 QLSLNNSQLPTTVVFGREVTNVGDPVVVYNATIT-APPGVEITVEPRSMSFSRLLEKQSF 709
              +++   P  V F R VTNVG P   Y A++    P ++I++EP  + F  L EK+SF
Sbjct: 615 TTFVSSLD-PFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSF 674

Query: 710 KVVVKASPLSAGKMVSGSLAWFDGRHVVKSPIVVYS 741
            V +    L  G  VS S+ W DG H V+SPIV YS
Sbjct: 675 VVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 690

BLAST of Sed0020013 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 529.6 bits (1363), Expect = 3.9e-150
Identity = 315/750 (42.00%), Postives = 459/750 (61.20%), Query Frame = 0

Query: 2   SIPKSSCLIVFVLFFIVACFAAVEINEKHFIVFLENNPVLNEVDAAETHLNVLMSVK-ES 61
           ++  SS L+  +L   ++  +AV  +++ +IV++ +     +      H+N+L  V  ES
Sbjct: 3   TLAASSSLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGES 62

Query: 62  HVEAKESMVYSYSQNFNAFAAKLSEEEAKTLSKREDVHYVIQNKYRELLTTRSWDFLGFP 121
            +E +  +V SY ++FN FAA+L+E E + ++K   V  V  NK  +L TT SWDF+G  
Sbjct: 63  SIEGR--LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 122

Query: 122 S--NATRNTKQESDIIVGLFDTGIAPTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKL 181
                 RN   ESD I+G+ D+GI P + SF D GFGPPPQKWKG C    NFT CN KL
Sbjct: 123 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT-CNNKL 182

Query: 182 IGARYFKLDDIPDPLDIISPIDVNGHGTHTSSTAAGNEISGANLGGLATGTARGGVPSAR 241
           IGAR +  +            D++GHGTHT+STAAGN +  A+  G+  GT RGGVP++R
Sbjct: 183 IGARDYTSE---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASR 242

Query: 242 VAMYKVCWVSTGCSDMDILAAFDAAIQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKS 301
           VA YKVC   TGCS   +L+AFD AI DGVD+I++SI       +  DPI+IGAF AM  
Sbjct: 243 VAAYKVC-TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAK 302

Query: 302 GIITVTAAGNAGPSAGSVINHAPWIVTVAASSIDRNFISPLELGNGNNISGVGINLLNLQ 361
           G++TV +AGN+GP   SV   APWI+TVAAS+ +R F++ + LGNG  + G  +N   ++
Sbjct: 303 GVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMK 362

Query: 362 QKMYPLVSGGDAARNQESVDGASFCDEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGASG 421
            K YPLV G  AA +    + A  C+   +D ++VK KI++C     G   IV+S+GA G
Sbjct: 363 GKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG--GPGGLKIVESVGAVG 422

Query: 422 AILQSDQFLDVTNIYMAPASMVSSFVGASIDNYIKSTRTPTAMIYKSRQL-EAAAPIVAS 481
            I ++ +  DV  I+  PA+ + +    S+ +Y++ST +P A++ K+  +    +P++AS
Sbjct: 423 LIYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIAS 482

Query: 482 FSSRGPNSGSKRILKPDIAAPGVNILAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHA 541
           FSSRGPN+ +  ILKPDI APGV ILAAY+P    +  + DT+  K++++SGTSM+CPH 
Sbjct: 483 FSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHV 542

Query: 542 AAAAAYVKSFHPLWSPAAIRSALITTATPSSRRLN--PDAEFAYGAGNLNPARAKNPGLV 601
           A  AAYVK+F+P WSP+ I+SA++TTA P +         EFAYG+G+++P  A NPGLV
Sbjct: 543 AGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLV 602

Query: 602 YDANEMSYIQFLCSEGYNGSAISILTGTKSIDCS---TLVPGQSHDSLNYPTFQLSLNNS 661
           Y+ ++  +I FLC   Y    + +++G +++ CS    ++P     +LNYP+    L+ S
Sbjct: 603 YELDKSDHIAFLCGMNYTSQVLKVISG-ETVTCSEAKKILP----RNLNYPSMSAKLSGS 662

Query: 662 QLPTTVVFGREVTNVGDPVVVYNATITAPPG--VEITVEPRSMSFSRLLEKQSFKVVVKA 721
               TV F R +TNVG P   Y + + A  G  +++ + P  +SF  + EKQSF V V  
Sbjct: 663 GTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTG 722

Query: 722 SPLSAGKMVSGSLAWFDGRHVVKSPIVVYS 741
           S L +    S +L W DG H V+SPIVVY+
Sbjct: 723 SNLDSEVPSSANLIWSDGTHNVRSPIVVYT 729

BLAST of Sed0020013 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 524.2 bits (1349), Expect = 1.7e-148
Identity = 317/722 (43.91%), Postives = 445/722 (61.63%), Query Frame = 0

Query: 27  NEKHFIVFLENNPVLNEVDAAETHLNVLMSVK-ESHVEAKESMVYSYSQNFNAFAAKLSE 86
           +++ +IV+L + P   E      H+++L  +  ES +E +  +V SY ++FN FAA+L+E
Sbjct: 31  DQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENR--LVRSYKKSFNGFAARLTE 90

Query: 87  EEAKTLSKREDVHYVIQNKYRELLTTRSWDFLGFPSN-ATRNTKQ-ESDIIVGLFDTGIA 146
            E K L+  E V  V  ++  +L TT SW+F+G      T+ T+  ESD I+G+ D+GI 
Sbjct: 91  SERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIY 150

Query: 147 PTADSFKDDGFGPPPQKWKGSCLHFANFTGCNKKLIGARYFKLDDIPDPLDIISPIDVNG 206
           P +DSF D GFGPPP+KWKG+C    NFT CN K+IGAR +      +     +  D +G
Sbjct: 151 PESDSFSDQGFGPPPKKWKGTCAGGKNFT-CNNKVIGARDYTAKSKANQ----TARDYSG 210

Query: 207 HGTHTSSTAAGNEISGANLGGLATGTARGGVPSARVAMYKVCWVSTGCSDMDILAAFDAA 266
           HGTHT+S AAGN ++ +N  GL  GTARGGVP+AR+A+YKVC  + GC    +++AFD A
Sbjct: 211 HGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVC-DNEGCDGEAMMSAFDDA 270

Query: 267 IQDGVDVISVSIAGNGFVNYSEDPISIGAFRAMKSGIITVTAAGNAGPSAGSVINHAPWI 326
           I DGVDVIS+SI  +    + EDPI+IGAF AM  G++TV AAGN GP   +V + APW+
Sbjct: 271 IADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWV 330

Query: 327 VTVAASSIDRNFISPLELGNGNNISGVGINLLNLQQKMYPLVSGGDAARNQESVDGASFC 386
            +VAAS  +R F++ + LG+G  + G  +N  ++    YPLV G  AA +  SVD A  C
Sbjct: 331 FSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLC 390

Query: 387 DEGSIDPTKVKDKIVMCKVMAKGADSIVKSLGASGAILQSDQFLDVTNIYMAPASMVSSF 446
           +   +D   VK KIV+C    KG     K LGA G+I+++ +  D   I   P S +S+ 
Sbjct: 391 EPKCLDGKLVKGKIVLCD-STKGLIEAQK-LGAVGSIVKNPE-PDRAFIRSFPVSFLSND 450

Query: 447 VGASIDNYIKSTRTPTAMIYKSRQL-EAAAPIVASFSSRGPNSGSKRILKPDIAAPGVNI 506
              S+ +Y+ ST+ P A + KS ++    AP+VASFSSRGP+S    ILKPDI APGV I
Sbjct: 451 DYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEI 510

Query: 507 LAAYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHAAAAAAYVKSFHPLWSPAAIRSALIT 566
           LAAY+P  S T  + DT+  K++++SGTSMACPH A  AAYVK+FHP WSP+ I+SA++T
Sbjct: 511 LAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMT 570

Query: 567 TATP--SSRRLNPDAEFAYGAGNLNPARAKNPGLVYDANEMSYIQFLCSEGYNGSAISIL 626
           TA P  +S       EFAYG+G+++P  A NPGLVY+  +  +I FLC   Y    + I+
Sbjct: 571 TAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRII 630

Query: 627 TGTKSIDCSTLVPGQSHDSLNYPTFQLSLNNSQLPTTVVFGREVTNVGDPVVVYNATITA 686
           +G  S  C+  +      +LNYPT    ++ ++ P  + F R VTNVG     YNA +  
Sbjct: 631 SGDNS-TCTKEISKTLPRNLNYPTMSAKVSGTK-PFNITFQRTVTNVGMQKSTYNAKVVK 690

Query: 687 PPG--VEITVEPRSMSFSRLLEKQSFKVVVKASPLSAGKMVSGSLAWFDGRHVVKSPIVV 741
            PG  + I V PR +S   + EKQSF V V +  +   + VS +L W DG H V+SPI+V
Sbjct: 691 FPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIV 739