Sed0019949 (gene) Chayote v1

Overview
NameSed0019949
Typegene
OrganismSechium edule (Chayote v1)
DescriptionAlanine--tRNA ligase
LocationLG01: 4699766 .. 4709231 (-)
RNA-Seq ExpressionSed0019949
SyntenySed0019949
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAATGATAATTTCCCACGCTACCAAAACATTTGGTCCTCTCAATCAAAACTTATCTTCTCCTTCCTCCTCACTCCAAATATATAAAAACCTCACTTCCAAATTCATGGGATAAATTCCAGGGTTCTGGAGCACTGCACGTCAATGGAGTTCACCTTGAAGCTTCCAAATCATCTTCTTCGTAGTTATGGCGACAAATCTCTTACTCCATTTCGACGTTCAAATCTGTGCGGCCACAAACCTGCCTCCCTTGCTCGCCCCAGACGGAAATTTTTCTTCTCTTCCGGTACTTCTCCGATTCTGTTCTATCATTTTTCCAATTTAGAGCTTCCGAATGATTTCAATCCGTGAAATGCAGTGTAGCGTTTTAGATTCTGAGGCGGGAATTTTATGTGGTTGTTGTTAGTTTAAGTGTTACTAAATCAGAACGGCAATGAAATTGTAAAGAAAACATGAGAGAAAGATGCTCATAGATTTACGTGGTTCTCTGAGTATGTGTTAGTTACATCCACGATCAGACGGGAGAACGGTTTTATTAAGAAGAATACAAAAATTAGAAGAGTTTTACTAAGGAGAATACAAAATTAAGATTTTAGGAGAGTTTATACACTGCAATCTCATGAATCTTATTACAAAATGGATCCAACTACAGAAAAGATCATATACGAAAATATAAACCCAATTACCTATTTCGTCCCCTCCTCACCAGTAGCCTTGCCCCTGGACCCCGTCTTTCTGGCAGCGAGACCCCGACTAAGTTATCGTGGGGATCAAATAGTTCAATGTATCTCTCAACATTAAATATCCGAATCCTCTGTATCAGAATGCAAGTAAAAATTATCGAGAGTTTGTTTCGAAGCAGTCTATCCGCTCAAACAATGGTTGAGTTATTGGATTCTTGCTGTGATTTATGAAACTCACTTTATTGTTTGGAGTCTTTGAACTTCAATAATATTCTCTGGTGCATTTTGTTATCTGTAACTGCACATGCTATTTGCTTTGACTACCAGAAAATTGTTGCTGCCTGTTTCAAACGAGCTATTGCACCATGTAATTTAAATCTGCTTTGATTAAATGGTTCACCAAGAAATGACGTAGTATACTCCATATGCGTGCAAGATGATATGGGTCTGTTTTGTTGGGCACAAAATGTTTAACCTGATTTAACATGAACATCAGAATTTTAATCCTGCTGGCGAGTCCCTAGTTCATTTGGATTGTAGTATTGTTGGTTGTGTTCATTTGATACTAGACTGTTGTGATTTCATTTTCATGAATCATGAAGAAATATTGGTGTGTGCTACTTCTAGTAGGATCCTTAACTCCTTTGTTGTGTTCTTCCGGCTGACCTATTATGTTCTGAATATCAGCTTTCGGGATATTACAGGCTATATTACGAAGAATTCTGCCCCTTTCCCTTCCACTATGCATTGTCAAAGACATTTTCTTCCAAAAGAAGCTCGGGGCACACAACTGAAGACAAGAAGTATATCAGTTAGTTTGATTAAAATTTATAATCTGGACTTGCTGATCATCATCTGCCTTTTCGAACCTTCATGAAATTCTTACCTTTTGACATTTCACTGTTTCAGCATCTGTGAAACCAGTGAATGAGGAATTGGTAGAGGATAAGTCCAAGGATCTCCAAATGACTGGGGACTCTATTCGTCGTAGGTTCCTTCAATTTTATGCTTCCCGTGGACACAAGGTTCTTCCCAGTGCTTCTTTGGTACCAGAAGATCCCACTGTTTTGTTGACAATTGCAGGAATGCTTCAATTCAAGCCTACATTCCTTGGAAAAGTATGACATGCACATGATTAAAGTTCATGTTACGTTCTGTTTTGTTAAAATGGGCTGTGATTATCTTTCTTTCACCATCCTTTTGCTTGGAAGCATTTGAAGATAACATTGTTATGGTGTGCTTGTTGTCTTCTCTCTTTATTTTGACTGTAATATTCAGTTGCACATTGTCCATCCTTTCAAAGACAACCTAGGCTCTGGTGTAATGTGGTAAGGTCACGCTTTGAGGGATATATCTAGGAAGGAGCAAGAGTACTAAGGATCTTGGATAGGGAATTTGTTATTTCTAGGACATGTTTTGCATTGTTTCTTTCTCTTGCAACATCTTACAAAAGAACTATTGTAGTTCCTTCTATGATCGACACGACACCCACCAGAGGGCTTTTTGTTATCTTTTTGGTTCGATGCTCTCTCTTAGTATTAATGGAACCTTGTTTCTCAACCAGAAAACTCCAAAGCCATAGCAAATTTTTAATGGTGCTATGGTTGTAGACTTACTAGGGCATGTATGGCCAATTGATCAAAACCTTTTTGAGACTATGGGTTTTCTTTTCTTAGTGCTAACTAGAAGGCTTTCTTGTAACTACCTAAATGACAGCCTGGCCTGATCGTTTTTTCAATGAAATATTTTCTTATCCAAACAAGGAATTTGTGGCCAATAACCCACAATACAACATTCTCTTTTGGTGTTGATTGTCATAATTTCTTCAAATTTTCTTCCGCTTCTCTATCACTTGTCAAAGGCCTTCTGACTCGGGCTACAGCTGGAGTTTCTTTGCTTCACACCTTATTAAAAAATCTTTATATTCGCTGTTAAATTAAAGTATCTGGGGAGAACATGTAGCCGATTCTCAGATACTTATAAAAAAAGTACCTGTTAATATAGTACGAAGTGACCAATCTCACTGTTCTTTGAATTGGAATTAGCTTATCGGAAGACTTTGATGCTACAGTTTCTTATGACTGGCTTCTTAATGATTGCATGGAGCATCGAGTTTCTTATGTTATGGGAAAAGTGAGGATCTGAGGAGTACGAAATTGGACTCTGTAGACTGGGTATCTAACACTCTCCTCTTGTTGAACACTCCAACATTTAATTATTATAGTTTTAATTACAACTTTCACGTCATTTGCACCTCCCAATTTTTTTGGAAAATGGTGTCATGTCTTCCTGTAGTGACTTCTGTATCTGAATGTTTAGTAGAACAGGAACTTGTAGCAGCAAAGATCCTTTACAGCCTACACGTATAATAATGCACATTCTCCCTTTCTTTTGTTGGATTTTCTCATGTATTCTTTAGTTTGTTGTTTTTATTCTTGAAAAATAATTGTTAACGTCATATCTTGAGCTAGTTTGAATATCAAAATCTTTAGCTTGTTCTTTATTTTTTTATACTGATTTTCAGGTACCTAGACTGGTACCTTGTGCTGCAACTTCACAAAGGTGCATACGTACCAATGATGTGGAGAATGTAGGTCGAACGGCTAGGCATCATACGTTCTTTGAGATGCTTGGAAACTTTAGTTTTGGTGATTACTTCAAGAAAGAAGCGATTAAGTGGGCATGGGAACTGACAACCATCGAGTATGAATGAATTACATCATCACAATGTTTGAGTATTCTAGAAAAAGTTCTAAAAAATATCAAGCTCGTTTGAAACTGAAGCATGTCGAGATTTCTCATCAAATAAAATATCATAATGCTGCTGAGAACATCCAAGCTATTAAGTTTCTTGAGCATAAAATTCTAGCGTTTTTTTTCCATATTAGCAACTTCATCCTGACAGTTTGAATGTGTATCATCATTATTTATTTACCAGTATCCCATCTCCCTTCAAACCTTATCTTTTTATTTAATCACAATTTCTATTCTAAGTATGGTGATCTGAGACAGTGTGATCAATACATAAACATGTCATTGAGTATAGTGCTTTAGATTTCATTGGATGATAGAGGGAATTTCATATTAAAAAAGCCGAATTACAGTTGGATTGAGCATTGATTAACTTAAGCTGAGTTCTTTCACTATCGCTAACAATAAGAAAAACTTACTCTAGTGTATTTTTATCCTTCCAGGTTTGGGTTGCCAGCCAACAGACTATGGATTAGTATATATGAAGAAGATGATGAAGCTTTTGCAATATGGCATGACGAGGTAGTAAAATGAAATCTTTCAACTTATTATGCTTAGTGACTATATGCTCTCTCTTTGATTGTATGTTTCTGTTTTTAAAAAACGTATTTTTATTACTTGCTCCTGCAACATAAAGCTGTAGCATGTTCAATGAAGTAAGGGACGTTGGTGATTATTTCTTGCTCTTACGTCTGGAGCCCATGACACCTTCTGCTGTCAAATATACATGTTACCTACTTAAACTTCTCTGAATTCTCTCCATGTAGATAAAAATGCATGCCATATTTTTCTTATTATTAGAAATTCCCTTGTAGCAGAAGATTATTGTCATGTAATGTATTCAAAATCTACAATTATTATTTTGATTTTTTGATAAATTTCGTTTAATTATTTGTATATGAATGGAGGCAAAAAAATAATTTTAGAAATCTAGGCAACCTTTATACTTAGGGGCGCTTGGGCCTTCGTCAGACAACGAGTTATAATATCCAGTGGTTATGCCATAACCTATCATCTCAACTATAATAATTCTCTTACCATGTTATAATTAAATTCTATGTTATAGTAATCCTTCCGTTATTAACCATTATGGAGTGGCCTATTTGTCAAGAGGGTCTTGGGGAGCTAAGAGGTGATGAGTTCAATCTATAGTGGCCACCTACCTTGAAAATAATTTCCTCCGAGTTTCCTTTATATCCAAATGTTTTAGGGTCAGACGGTAATCCGTCGAGGTGTGGCAAGTTGGCTTAGACAACCGCAAATATAAAAAAAGAGGTAATCTTTATGTTATATTAACCTGCATCACAAATGTAGGTTATTACAATCTCCCCTATCATAATAACTCCGTTTATGGTTTTGAGAGTATGCTCCCCTCAAAGTCCTAGGTTTGAGACTCACCTATGACATTACCCCTTCGATGTCTTCGGTGCCTGGCTTAGGGACGGGCGTGGATTACGTTTACCTTGTTTCAAAATAAATAAATAAATAACTCTGTTTATGATAACCCAACTGTTGCCCCAAACACTTTTAATGTATAACTAATTTCCTTCCAGGTAGGCGTTCCAGTCGATCGTATAAAGAGGATGGGCGAAGAAGACAACTTTTGGACTAGTGGCATCACGGGACCATGTGGCCCATGCTCTGAGATTTATTATGATTTCCAACCTGAAAAGGGGTATTCTGATGTTGTATGTGGTTTTTTCTCTCTCTTTTGTTGATTTCAGTAAGTCTATGAACTAGTTGTCTAGTTCATACTAGTATTTCATTCCCCTTTAAGTTTTTGAAAAGTTGATTCTTTTACAGGATTTGGGAGATGATACAAGGTTTATGGAGTTCTACAACTTAGTTTTCATGCAGTATAACAAAAAGGATGATGGGTCCCTTGAACCTTTGAAACAGAAGAATATAGATACAGGACTAGGTTTAGAGCGCATGGCTCGCATTCTCCAAAAAGTATTATAGCTACTCATTGTAAAGTTACTTATGAGGTTCAACATGATGTTTTTATTTTTCCTCACTTTTTAAGTTTTGTTTTTTTTTTTGTGAAACACACTTTTTAAGTTATTATAGGATAACTCACTGAACTAGAAAATCTTGTGTTTCAAGTAACTCTATACTATTTTATGGTACAATCATTTGCTGCTCATGTTGGTCAACGTTTGTCGATTCCCATTTTCTTGCAGGTTCCAAACAACTATGAGACTGACTTGATCTACCCCATTATACAGACGGCCTCAGAGTTAGCAAATGTGATATATGACACTGCTGATAATCAATCAAAAACAAACCTTAAAGTATGTGTATCTCTTAGAAGAATTTAGGCTTCATTCCACTTTGATTCTTCTCCATTCTAAAACAAACCTTAAAGTATGTTGTTACTTCAATGCTTTGTATTGCTTCTAGATCATTGGAGATCATATGCGTGCAGTTGTGTACCTGATATCTGATGGAGTCGTCCCATCCAATATTGGAAGAGGATATGTTGTCCGCAGGCTTATCCGAAGAGTCGTTCGTACTGGTAGACTTCTTGGAATAAAGGGGGATGGAAGAGGAAATATTGATGGGGCATTTACACCGCTCATTGCCGAAAAGGTCATTGCATTAAGCTCCCACATTGACGAGGACGTGAAGAGTAGGGAAGGTCGCATTCTTGAGGAGTTGAAAAGGGAAGAACTTAGGTTTGTACAGACACTAGAGCGAGGGGAAAAGCTGCTTGAAGAAATGCTAGCTGATGCATTGGCAAGTGCTAAAGAAGATGGAAGGGTTCCTTGTTTGGTTGGTAAAGATGCATTTCTGTTGTACGATACATATGGGTTTCCAGTGGAGATTACCACAGAAGTTGCTCAAGAGCGTGGTGTAGTGGTTGATATGAAGGGTTTCGATACTGAAATGGATAACCAGAGGCGACAATCTCAGGCTGCACACAATGCTGTCAAATTGGAAGTTGGAGAAGGTGCAGATATCATGGGAAACATCTCCCACACAGAATTTCTTGGATATGATTTGCTCTCTGCTAAAGCAATTGTGGAGAGCCTTATTGTGAATGGTACTTCTGTGTTACAGGTTTCAGAAGGAAATGACGTAGAAATTTTGTTGAATAGGACACCTTTCTATGCTGAATCTGGTGGTCAGATTGGAGACCATGGTTTCATCTATATTTCAGAAGGTGAAAACAGACAAAATGTAGTAGTGGAGGTAAAAGATGTACAAAAATCCGCGGGCAGCATATTTATTCACAAGGGTACCATCAAACAGGGAGTTCTAGAGGTTGGAAGAGAAGTGGAAGCAGAAGTAGATACAAAGTTGAGGCAGGGAGCCAAGGTAGCATTTATAAGAAATTTATTATTTTCTTTTTTTCTTTTGATATCCGTGACTGTCCAGGTCAGCTTGCGCACACCTCGACTATTGTCACGGGACATTATTGCCTGACCCTACAACATTTGGATGACAAGAAAACTCATAAAAAATTAATTCCTAGGTAGGTGGCTACCATGGATTGAACTCATCATCTCTTAGTTTTTCAAGACCCTCTTAACCACTATGTCACTCCATGATGGTTTATACGAAGTTTGTAGAAATAGCTTTATGAGGAACACCTTTGACTTTCTTTTAAAATTTTGTAGGTTCATCATACTGCTACGCATTTGCTACAATCTGCACTTAAGAGGGTTATAGGCCAAGAAACATCACAAGCTGGTTCCTTGGTTGCCTTTGACCGGCTCAGATTTGACTTCAGCTACCACCGACCTCTTGCTGACACTGAGATCTTAAAAATTGAAGAGTTGATCAATGGTTGGATTGGAGATGCTGTGCTTCTTCAAACTAAAGTTATGCCTCTTGTTGATGCCAAGAGAGCTGGGGCTGTTGCTATGTTTGGAGAGAAGTATGGAGAAGAGGTCTGAACTTCTCATTATAACCTCACCTGAAATCACATTTTCATTTTGTTTCTGTTAAGGGTTGGTTAGTTTGGATCATTTTCACAAAACATGCACATTATTATTTACAAAAGACGACTCGCTTGACCATATTGTAATGTAATTAAAAGTTCAGTAAACCTATAGCAATGTGAAGCTTCAAATATTATTTTATTTTTTAATTTCTGGCTAAAAGAAGGATTTATTTTTCTCTCAAACAACACTAGAGAATCTTATAGTTGTTAGCTAGAAACTGGCAATCGCTTCTAAGTTTGATGTAAATATGTTTATCGTGATGAACCGCATGAAATTTGTTACTTAACATGGATCTCTTTTATCTTTCTAGGTACGTGTTGTTGAGGTTCCTGGTGTATCAATGGAACTCTGTGGTGGGACTCATGTATTCAATACTTCAGAGATACGAGGCTTTAAAATAATCTCAGAGCAGGGCATTGCATCTGGCGTTAGACGAATAGAGGCTGTGGCTGGTGACGCTTTCATCGAGTACATTAATGCCAGAGACTACCATATGAAGCGGCTGTGCACCATGCTCAAAGTACGCTCTTCGCTTAGACACTTAGTCATGACACTGGAAATTGTATTATAATTGCATTACCTTTTCAGTATCATAACTAAGTTTAAACTTGATTGGCGGGTATACTGAATTCGTACAACATTCTTTTGGAAAGAGAACTTTACTTTTTCCTGTGTGCAGGTAAAAGCTGAAGATGTTACAACCCGGGTAGAAAATCTTGCCGAGGAATTGCGGTTGGCGAGAAATGAAATCTCTAATTTGCGAGAAAAAACTGCAGTTTTTAAAGCATCTAGCATTGCTAATAAGGCATTTGTGGTTGGAACTTCAAAAGAGATCAGGTGAATAATTATAAACCTTACCAAGTATCCATTCTCTTGTGGTCTAGTCTCTAGGGGGTGTTTGTTTGTTGAATTTAGGGAGATATTATAACTGCTCAAAACAATTCCTCATTTTTACTAAAAATAACTTTTCATTTCTAACTACTCAAAATAACTAATTTTTAACCATACATTTCTAATAACTTTTTTATCTACTCAAAATAACTATTCATTTCAAATCCACTTACAAACAAGCTTTTATCTCTAACTCTACAAAAAAAAATTATATTCAAACGCGTTTTTGGCACAACTGTCAATTTCTAACTCTTCAAAATAACTTGTTTAAAAATAAATAAATAAAAACTCTTCAAACTAATTCTTCATCCAAAACCTAATCCAAACACCTCCAAGAGGTGTTGCGTTAGCTGGATGAACATCCAACTCCATATTTCCTTGGTTTTCTTTATCAAGTTTTAGACATTTTGAAACAACATTCTTCAGATGAACTTTACAAAAGAGAACAATCCATCTAAGTTTAACAAATAACGGTCTCTTTCTCAGGGTACTAGTTGAGTGTATGGACGACACCGATGCCGATTCACTGAAAGCTGCGGCTGAGTTCCTGATAGACAATCTTCAAGATCCAGCAGCAATTGTACTAGGCTCCTGTCCCGGCGAAGGAAAAGTGAGTTTAGTTGCAGCATTTACACCAACTGTTGTTGATCTAGGAGTACAGGCAGGGAAGTTCATAGGACCTATAGCTAAACTGTGTGGTGGGGGAGGGGGTGGCAGGCCCAATTTTGCTCAGGCAGGTGGAAGAAAGCCTGAGAACTTATTGAATGCACTCGAAAACGCTCGATCAGAGCTCATCCACATCCTATCTGAGAAGGCAAGCTAGAACTCATCCGAATCGCAAATGGAACACATCCGTGGCTTCGAGGTTCAGAGCTTGTAACCTTGAAAATGGCTGTGGTAAGGCAACAGTTCTCCAAACTGGTAAAGTATGTTTGGAAGAAGCAAGGATCTAAAATGTAATAGATCACTGGTCAACTTTCAGAGTTACTTGTTACTTAATGTTACCTGGTTCGTTGAATGAAACTCTCAACACATTTGGAAAAAGAAAAAAAAAAGCAGTTATCTCTAAAAGAATTGATTGTTACTCATGCTGTAGAGGAAGAATAAGTTGTGTTTAAATTGGGATTTTGGGTTAATTCTGTATGGAATTATTAATCTGTTCAGCTAACTTTATATAAATTTATAGTT

mRNA sequence

AAAAAAAATGATAATTTCCCACGCTACCAAAACATTTGGTCCTCTCAATCAAAACTTATCTTCTCCTTCCTCCTCACTCCAAATATATAAAAACCTCACTTCCAAATTCATGGGATAAATTCCAGGGTTCTGGAGCACTGCACGTCAATGGAGTTCACCTTGAAGCTTCCAAATCATCTTCTTCGTAGTTATGGCGACAAATCTCTTACTCCATTTCGACGTTCAAATCTGTGCGGCCACAAACCTGCCTCCCTTGCTCGCCCCAGACGGAAATTTTTCTTCTCTTCCGGTACCTAGACTGGTACCTTGTGCTGCAACTTCACAAAGGTGCATACGTACCAATGATGTGGAGAATGTAGGTCGAACGGCTAGGCATCATACGTTCTTTGAGATGCTTGGAAACTTTAGTTTTGGTGATTACTTCAAGAAAGAAGCGATTAAGTGGGCATGGGAACTGACAACCATCGAGTTTGGGTTGCCAGCCAACAGACTATGGATTAGTATATATGAAGAAGATGATGAAGCTTTTGCAATATGGCATGACGAGGTAGGCGTTCCAGTCGATCGTATAAAGAGGATGGGCGAAGAAGACAACTTTTGGACTAGTGGCATCACGGGACCATGTGGCCCATGCTCTGAGATTTATTATGATTTCCAACCTGAAAAGGGGTATTCTGATGTTGATTTGGGAGATGATACAAGGTTTATGGAGTTCTACAACTTAGTTTTCATGCAGTATAACAAAAAGGATGATGGGTCCCTTGAACCTTTGAAACAGAAGAATATAGATACAGGACTAGGTTTAGAGCGCATGGCTCGCATTCTCCAAAAAGTTCCAAACAACTATGAGACTGACTTGATCTACCCCATTATACAGACGGCCTCAGAGTTAGCAAATGTGATATATGACACTGCTGATAATCAATCAAAAACAAACCTTAAAATCATTGGAGATCATATGCGTGCAGTTGTGTACCTGATATCTGATGGAGTCGTCCCATCCAATATTGGAAGAGGATATGTTGTCCGCAGGCTTATCCGAAGAGTCGTTCGTACTGGTAGACTTCTTGGAATAAAGGGGGATGGAAGAGGAAATATTGATGGGGCATTTACACCGCTCATTGCCGAAAAGGTCATTGCATTAAGCTCCCACATTGACGAGGACGTGAAGAGTAGGGAAGGTCGCATTCTTGAGGAGTTGAAAAGGGAAGAACTTAGGTTTGTACAGACACTAGAGCGAGGGGAAAAGCTGCTTGAAGAAATGCTAGCTGATGCATTGGCAAGTGCTAAAGAAGATGGAAGGGTTCCTTGTTTGGTTGGTAAAGATGCATTTCTGTTGTACGATACATATGGGTTTCCAGTGGAGATTACCACAGAAGTTGCTCAAGAGCGTGGTGTAGTGGTTGATATGAAGGGTTTCGATACTGAAATGGATAACCAGAGGCGACAATCTCAGGCTGCACACAATGCTGTCAAATTGGAAGTTGGAGAAGGTGCAGATATCATGGGAAACATCTCCCACACAGAATTTCTTGGATATGATTTGCTCTCTGCTAAAGCAATTGTGGAGAGCCTTATTGTGAATGGTACTTCTGTGTTACAGGTTTCAGAAGGAAATGACGTAGAAATTTTGTTGAATAGGACACCTTTCTATGCTGAATCTGGTGGTCAGATTGGAGACCATGGTTTCATCTATATTTCAGAAGGTGAAAACAGACAAAATGTAGTAGTGGAGGTAAAAGATGTACAAAAATCCGCGGGCAGCATATTTATTCACAAGGGTACCATCAAACAGGGAGTTCTAGAGGTTGGAAGAGAAGTGGAAGCAGAAGTAGATACAAAGTTGAGGCAGGGAGCCAAGGTTCATCATACTGCTACGCATTTGCTACAATCTGCACTTAAGAGGGTTATAGGCCAAGAAACATCACAAGCTGGTTCCTTGGTTGCCTTTGACCGGCTCAGATTTGACTTCAGCTACCACCGACCTCTTGCTGACACTGAGATCTTAAAAATTGAAGAGTTGATCAATGGTTGGATTGGAGATGCTGTGCTTCTTCAAACTAAAGTTATGCCTCTTGTTGATGCCAAGAGAGCTGGGGCTGTTGCTATGTTTGGAGAGAAGTATGGAGAAGAGGTACGTGTTGTTGAGGTTCCTGGTGTATCAATGGAACTCTGTGGTGGGACTCATGTATTCAATACTTCAGAGATACGAGGCTTTAAAATAATCTCAGAGCAGGGCATTGCATCTGGCGTTAGACGAATAGAGGCTGTGGCTGGTGACGCTTTCATCGAGTACATTAATGCCAGAGACTACCATATGAAGCGGCTGTGCACCATGCTCAAAGTAAAAGCTGAAGATGTTACAACCCGGGTAGAAAATCTTGCCGAGGAATTGCGGTTGGCGAGAAATGAAATCTCTAATTTGCGAGAAAAAACTGCAGTTTTTAAAGCATCTAGCATTGCTAATAAGGCATTTGTGGTTGGAACTTCAAAAGAGATCAGGGTACTAGTTGAGTGTATGGACGACACCGATGCCGATTCACTGAAAGCTGCGGCTGAGTTCCTGATAGACAATCTTCAAGATCCAGCAGCAATTGTACTAGGCTCCTGTCCCGGCGAAGGAAAAGTGAGTTTAGTTGCAGCATTTACACCAACTGTTGTTGATCTAGGAGTACAGGCAGGGAAGTTCATAGGACCTATAGCTAAACTGTGTGGTGGGGGAGGGGGTGGCAGGCCCAATTTTGCTCAGGCAGGTGGAAGAAAGCCTGAGAACTTATTGAATGCACTCGAAAACGCTCGATCAGAGCTCATCCACATCCTATCTGAGAAGGCAAGCTAGAACTCATCCGAATCGCAAATGGAACACATCCGTGGCTTCGAGGTTCAGAGCTTGTAACCTTGAAAATGGCTGTGGTAAGGCAACAGTTCTCCAAACTGGTAAAGTATGTTTGGAAGAAGCAAGGATCTAAAATGTAATAGATCACTGGTCAACTTTCAGAGTTACTTGTTACTTAATGTTACCTGGTTCGTTGAATGAAACTCTCAACACATTTGGAAAAAGAAAAAAAAAAGCAGTTATCTCTAAAAGAATTGATTGTTACTCATGCTGTAGAGGAAGAATAAGTTGTGTTTAAATTGGGATTTTGGGTTAATTCTGTATGGAATTATTAATCTGTTCAGCTAACTTTATATAAATTTATAGTT

Coding sequence (CDS)

ATGGCGACAAATCTCTTACTCCATTTCGACGTTCAAATCTGTGCGGCCACAAACCTGCCTCCCTTGCTCGCCCCAGACGGAAATTTTTCTTCTCTTCCGGTACCTAGACTGGTACCTTGTGCTGCAACTTCACAAAGGTGCATACGTACCAATGATGTGGAGAATGTAGGTCGAACGGCTAGGCATCATACGTTCTTTGAGATGCTTGGAAACTTTAGTTTTGGTGATTACTTCAAGAAAGAAGCGATTAAGTGGGCATGGGAACTGACAACCATCGAGTTTGGGTTGCCAGCCAACAGACTATGGATTAGTATATATGAAGAAGATGATGAAGCTTTTGCAATATGGCATGACGAGGTAGGCGTTCCAGTCGATCGTATAAAGAGGATGGGCGAAGAAGACAACTTTTGGACTAGTGGCATCACGGGACCATGTGGCCCATGCTCTGAGATTTATTATGATTTCCAACCTGAAAAGGGGTATTCTGATGTTGATTTGGGAGATGATACAAGGTTTATGGAGTTCTACAACTTAGTTTTCATGCAGTATAACAAAAAGGATGATGGGTCCCTTGAACCTTTGAAACAGAAGAATATAGATACAGGACTAGGTTTAGAGCGCATGGCTCGCATTCTCCAAAAAGTTCCAAACAACTATGAGACTGACTTGATCTACCCCATTATACAGACGGCCTCAGAGTTAGCAAATGTGATATATGACACTGCTGATAATCAATCAAAAACAAACCTTAAAATCATTGGAGATCATATGCGTGCAGTTGTGTACCTGATATCTGATGGAGTCGTCCCATCCAATATTGGAAGAGGATATGTTGTCCGCAGGCTTATCCGAAGAGTCGTTCGTACTGGTAGACTTCTTGGAATAAAGGGGGATGGAAGAGGAAATATTGATGGGGCATTTACACCGCTCATTGCCGAAAAGGTCATTGCATTAAGCTCCCACATTGACGAGGACGTGAAGAGTAGGGAAGGTCGCATTCTTGAGGAGTTGAAAAGGGAAGAACTTAGGTTTGTACAGACACTAGAGCGAGGGGAAAAGCTGCTTGAAGAAATGCTAGCTGATGCATTGGCAAGTGCTAAAGAAGATGGAAGGGTTCCTTGTTTGGTTGGTAAAGATGCATTTCTGTTGTACGATACATATGGGTTTCCAGTGGAGATTACCACAGAAGTTGCTCAAGAGCGTGGTGTAGTGGTTGATATGAAGGGTTTCGATACTGAAATGGATAACCAGAGGCGACAATCTCAGGCTGCACACAATGCTGTCAAATTGGAAGTTGGAGAAGGTGCAGATATCATGGGAAACATCTCCCACACAGAATTTCTTGGATATGATTTGCTCTCTGCTAAAGCAATTGTGGAGAGCCTTATTGTGAATGGTACTTCTGTGTTACAGGTTTCAGAAGGAAATGACGTAGAAATTTTGTTGAATAGGACACCTTTCTATGCTGAATCTGGTGGTCAGATTGGAGACCATGGTTTCATCTATATTTCAGAAGGTGAAAACAGACAAAATGTAGTAGTGGAGGTAAAAGATGTACAAAAATCCGCGGGCAGCATATTTATTCACAAGGGTACCATCAAACAGGGAGTTCTAGAGGTTGGAAGAGAAGTGGAAGCAGAAGTAGATACAAAGTTGAGGCAGGGAGCCAAGGTTCATCATACTGCTACGCATTTGCTACAATCTGCACTTAAGAGGGTTATAGGCCAAGAAACATCACAAGCTGGTTCCTTGGTTGCCTTTGACCGGCTCAGATTTGACTTCAGCTACCACCGACCTCTTGCTGACACTGAGATCTTAAAAATTGAAGAGTTGATCAATGGTTGGATTGGAGATGCTGTGCTTCTTCAAACTAAAGTTATGCCTCTTGTTGATGCCAAGAGAGCTGGGGCTGTTGCTATGTTTGGAGAGAAGTATGGAGAAGAGGTACGTGTTGTTGAGGTTCCTGGTGTATCAATGGAACTCTGTGGTGGGACTCATGTATTCAATACTTCAGAGATACGAGGCTTTAAAATAATCTCAGAGCAGGGCATTGCATCTGGCGTTAGACGAATAGAGGCTGTGGCTGGTGACGCTTTCATCGAGTACATTAATGCCAGAGACTACCATATGAAGCGGCTGTGCACCATGCTCAAAGTAAAAGCTGAAGATGTTACAACCCGGGTAGAAAATCTTGCCGAGGAATTGCGGTTGGCGAGAAATGAAATCTCTAATTTGCGAGAAAAAACTGCAGTTTTTAAAGCATCTAGCATTGCTAATAAGGCATTTGTGGTTGGAACTTCAAAAGAGATCAGGGTACTAGTTGAGTGTATGGACGACACCGATGCCGATTCACTGAAAGCTGCGGCTGAGTTCCTGATAGACAATCTTCAAGATCCAGCAGCAATTGTACTAGGCTCCTGTCCCGGCGAAGGAAAAGTGAGTTTAGTTGCAGCATTTACACCAACTGTTGTTGATCTAGGAGTACAGGCAGGGAAGTTCATAGGACCTATAGCTAAACTGTGTGGTGGGGGAGGGGGTGGCAGGCCCAATTTTGCTCAGGCAGGTGGAAGAAAGCCTGAGAACTTATTGAATGCACTCGAAAACGCTCGATCAGAGCTCATCCACATCCTATCTGAGAAGGCAAGCTAG

Protein sequence

MATNLLLHFDVQICAATNLPPLLAPDGNFSSLPVPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYYDFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQKVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNIGRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEITTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLLSAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVVVEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRVIGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAKRAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEAVAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFKASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKVSLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSELIHILSEKAS
Homology
BLAST of Sed0019949 vs. NCBI nr
Match: XP_023533450.1 (alanine--tRNA ligase, chloroplastic/mitochondrial isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1593.2 bits (4124), Expect = 0.0e+00
Identity = 799/849 (94.11%), Postives = 827/849 (97.41%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           VPRLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE
Sbjct: 91  VPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 150

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPANRLWISIYEEDDEAFAIWHDEVGVPV+RIKRMGE+DNFWTSGITGPCGPCSEIYY
Sbjct: 151 FGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCSEIYY 210

Query: 154 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 213
           DFQPEKG+SDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ
Sbjct: 211 DFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 270

Query: 214 KVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNI 273
           KVPNNYETDLIYPIIQ ASELA+V YDTADNQSKT+LKIIGDHMRA+VYLISDGVVPSNI
Sbjct: 271 KVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYLISDGVVPSNI 330

Query: 274 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRI 333
           GRGY+VRRLIRRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALSSHIDEDVK R GRI
Sbjct: 331 GRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVKGRTGRI 390

Query: 334 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEI 393
           LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGR PCL GKDAFLLYDTYGFPVEI
Sbjct: 391 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLYDTYGFPVEI 450

Query: 394 TTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLL 453
           TTEVAQERGVVVDMKGFD EMDNQRRQSQAAHNAVKLEVGEGADIM NIS TEFLGYD+L
Sbjct: 451 TTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEFLGYDML 510

Query: 454 SAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVV 513
           SAKAIVESLIVNG SVLQVSEG+DVE+LLNRTPFYAESGGQIGDHGFIYISEGENR NVV
Sbjct: 511 SAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGENRGNVV 570

Query: 514 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 573
           +EVKDVQKSAGSIF+HKGTIKQGVLEVGREV+AEVD KLRQGAKVHHTATHLLQSAL+R+
Sbjct: 571 MEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVHHTATHLLQSALRRI 630

Query: 574 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 633
           IGQETSQAGSLVAFDRLRFDF+YHRPLADTEIL+IEELINGWIGDAVLLQTKVMPL +AK
Sbjct: 631 IGQETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVMPLAEAK 690

Query: 634 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 693
            AGA+AMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFK+ISEQGIASGVRRIEA
Sbjct: 691 SAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGIASGVRRIEA 750

Query: 694 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 753
           VAGDAFIEY+NARDYHMKRLCTMLKVKAEDVTTRVENL E+LRLARNEISNLREKTAVFK
Sbjct: 751 VAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRLARNEISNLREKTAVFK 810

Query: 754 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 813
           ASS+ANKAFVVGTSKEIRVLVE MDDTDADSLK AAEFL+DNLQDPAAIVLGSCPGEGKV
Sbjct: 811 ASSLANKAFVVGTSKEIRVLVEYMDDTDADSLKGAAEFLMDNLQDPAAIVLGSCPGEGKV 870

Query: 814 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 873
           SLVAAFTPTVVDLGVQA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENAR+EL
Sbjct: 871 SLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARTEL 930

Query: 874 IHILSEKAS 883
           + ILSEKAS
Sbjct: 931 VRILSEKAS 939

BLAST of Sed0019949 vs. NCBI nr
Match: XP_023533449.1 (alanine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1593.2 bits (4124), Expect = 0.0e+00
Identity = 799/849 (94.11%), Postives = 827/849 (97.41%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           VPRLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE
Sbjct: 145 VPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 204

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPANRLWISIYEEDDEAFAIWHDEVGVPV+RIKRMGE+DNFWTSGITGPCGPCSEIYY
Sbjct: 205 FGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCSEIYY 264

Query: 154 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 213
           DFQPEKG+SDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ
Sbjct: 265 DFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 324

Query: 214 KVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNI 273
           KVPNNYETDLIYPIIQ ASELA+V YDTADNQSKT+LKIIGDHMRA+VYLISDGVVPSNI
Sbjct: 325 KVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYLISDGVVPSNI 384

Query: 274 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRI 333
           GRGY+VRRLIRRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALSSHIDEDVK R GRI
Sbjct: 385 GRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVKGRTGRI 444

Query: 334 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEI 393
           LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGR PCL GKDAFLLYDTYGFPVEI
Sbjct: 445 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLYDTYGFPVEI 504

Query: 394 TTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLL 453
           TTEVAQERGVVVDMKGFD EMDNQRRQSQAAHNAVKLEVGEGADIM NIS TEFLGYD+L
Sbjct: 505 TTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEFLGYDML 564

Query: 454 SAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVV 513
           SAKAIVESLIVNG SVLQVSEG+DVE+LLNRTPFYAESGGQIGDHGFIYISEGENR NVV
Sbjct: 565 SAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGENRGNVV 624

Query: 514 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 573
           +EVKDVQKSAGSIF+HKGTIKQGVLEVGREV+AEVD KLRQGAKVHHTATHLLQSAL+R+
Sbjct: 625 MEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVHHTATHLLQSALRRI 684

Query: 574 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 633
           IGQETSQAGSLVAFDRLRFDF+YHRPLADTEIL+IEELINGWIGDAVLLQTKVMPL +AK
Sbjct: 685 IGQETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVMPLAEAK 744

Query: 634 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 693
            AGA+AMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFK+ISEQGIASGVRRIEA
Sbjct: 745 SAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGIASGVRRIEA 804

Query: 694 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 753
           VAGDAFIEY+NARDYHMKRLCTMLKVKAEDVTTRVENL E+LRLARNEISNLREKTAVFK
Sbjct: 805 VAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRLARNEISNLREKTAVFK 864

Query: 754 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 813
           ASS+ANKAFVVGTSKEIRVLVE MDDTDADSLK AAEFL+DNLQDPAAIVLGSCPGEGKV
Sbjct: 865 ASSLANKAFVVGTSKEIRVLVEYMDDTDADSLKGAAEFLMDNLQDPAAIVLGSCPGEGKV 924

Query: 814 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 873
           SLVAAFTPTVVDLGVQA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENAR+EL
Sbjct: 925 SLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARTEL 984

Query: 874 IHILSEKAS 883
           + ILSEKAS
Sbjct: 985 VRILSEKAS 993

BLAST of Sed0019949 vs. NCBI nr
Match: XP_023533448.1 (alanine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1593.2 bits (4124), Expect = 0.0e+00
Identity = 799/849 (94.11%), Postives = 827/849 (97.41%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           VPRLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE
Sbjct: 151 VPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 210

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPANRLWISIYEEDDEAFAIWHDEVGVPV+RIKRMGE+DNFWTSGITGPCGPCSEIYY
Sbjct: 211 FGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCSEIYY 270

Query: 154 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 213
           DFQPEKG+SDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ
Sbjct: 271 DFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 330

Query: 214 KVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNI 273
           KVPNNYETDLIYPIIQ ASELA+V YDTADNQSKT+LKIIGDHMRA+VYLISDGVVPSNI
Sbjct: 331 KVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYLISDGVVPSNI 390

Query: 274 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRI 333
           GRGY+VRRLIRRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALSSHIDEDVK R GRI
Sbjct: 391 GRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVKGRTGRI 450

Query: 334 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEI 393
           LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGR PCL GKDAFLLYDTYGFPVEI
Sbjct: 451 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLYDTYGFPVEI 510

Query: 394 TTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLL 453
           TTEVAQERGVVVDMKGFD EMDNQRRQSQAAHNAVKLEVGEGADIM NIS TEFLGYD+L
Sbjct: 511 TTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEFLGYDML 570

Query: 454 SAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVV 513
           SAKAIVESLIVNG SVLQVSEG+DVE+LLNRTPFYAESGGQIGDHGFIYISEGENR NVV
Sbjct: 571 SAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGENRGNVV 630

Query: 514 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 573
           +EVKDVQKSAGSIF+HKGTIKQGVLEVGREV+AEVD KLRQGAKVHHTATHLLQSAL+R+
Sbjct: 631 MEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVHHTATHLLQSALRRI 690

Query: 574 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 633
           IGQETSQAGSLVAFDRLRFDF+YHRPLADTEIL+IEELINGWIGDAVLLQTKVMPL +AK
Sbjct: 691 IGQETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVMPLAEAK 750

Query: 634 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 693
            AGA+AMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFK+ISEQGIASGVRRIEA
Sbjct: 751 SAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGIASGVRRIEA 810

Query: 694 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 753
           VAGDAFIEY+NARDYHMKRLCTMLKVKAEDVTTRVENL E+LRLARNEISNLREKTAVFK
Sbjct: 811 VAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRLARNEISNLREKTAVFK 870

Query: 754 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 813
           ASS+ANKAFVVGTSKEIRVLVE MDDTDADSLK AAEFL+DNLQDPAAIVLGSCPGEGKV
Sbjct: 871 ASSLANKAFVVGTSKEIRVLVEYMDDTDADSLKGAAEFLMDNLQDPAAIVLGSCPGEGKV 930

Query: 814 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 873
           SLVAAFTPTVVDLGVQA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENAR+EL
Sbjct: 931 SLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARTEL 990

Query: 874 IHILSEKAS 883
           + ILSEKAS
Sbjct: 991 VRILSEKAS 999

BLAST of Sed0019949 vs. NCBI nr
Match: XP_038901232.1 (alanine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 1589.3 bits (4114), Expect = 0.0e+00
Identity = 801/849 (94.35%), Postives = 821/849 (96.70%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           VPRLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE
Sbjct: 151 VPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 210

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGE+DNFWTSGITGPCGPCSEIYY
Sbjct: 211 FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEDDNFWTSGITGPCGPCSEIYY 270

Query: 154 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 213
           DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ
Sbjct: 271 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 330

Query: 214 KVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNI 273
           KVPNNYETDLIYPIIQ ASELANV YDTADN+SKTNLKIIGDHMRA+VYLISDGVVPSNI
Sbjct: 331 KVPNNYETDLIYPIIQKASELANVTYDTADNKSKTNLKIIGDHMRAIVYLISDGVVPSNI 390

Query: 274 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRI 333
           GRGYVVRRLIRRVVRTGRLLGIKGDGRGNID AFTPLIAEKVIALS+HIDEDVK R GRI
Sbjct: 391 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDEAFTPLIAEKVIALSNHIDEDVKGRAGRI 450

Query: 334 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEI 393
           LEELKREELRFVQTLERGEKLLEEMLADALASAKE GRVPCL GKDAFLLYDTYGFPVEI
Sbjct: 451 LEELKREELRFVQTLERGEKLLEEMLADALASAKEGGRVPCLTGKDAFLLYDTYGFPVEI 510

Query: 394 TTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLL 453
           TTEVAQERGVVVDM+GFD EMDNQRRQSQAAHN VKLEVGEGADIM NIS TEFLGYD+L
Sbjct: 511 TTEVAQERGVVVDMEGFDIEMDNQRRQSQAAHNVVKLEVGEGADIMENISDTEFLGYDML 570

Query: 454 SAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVV 513
           SAKAIVESLIVNG  V QV+EGNDVEILLNRTPFYAESGGQIGDHGFIY SEGEN QNVV
Sbjct: 571 SAKAIVESLIVNGNPVSQVAEGNDVEILLNRTPFYAESGGQIGDHGFIYNSEGENSQNVV 630

Query: 514 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 573
           VEVKDVQKSAGSIF+HKGTIKQGVLEVGREVEA VD +LRQGAKVHHTATHLLQSALK++
Sbjct: 631 VEVKDVQKSAGSIFVHKGTIKQGVLEVGREVEAAVDAELRQGAKVHHTATHLLQSALKKI 690

Query: 574 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 633
           IGQETSQAGSLVAFDRLRFDF+YHRPL D EI+KIEELINGWIGDAVLLQTKVMPL DAK
Sbjct: 691 IGQETSQAGSLVAFDRLRFDFNYHRPLVDAEIMKIEELINGWIGDAVLLQTKVMPLTDAK 750

Query: 634 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 693
           RAGA+AMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA
Sbjct: 751 RAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 810

Query: 694 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 753
           VAGDAFIEY+NARDYHMKRLCTMLKVKAEDVTTRV+NL EELR+ARNEISNLREKTAVFK
Sbjct: 811 VAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVDNLLEELRIARNEISNLREKTAVFK 870

Query: 754 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 813
           ASS ANKA +VGTSKEIRVLVE MDDTDADSLK AAEFLIDNLQDPAAIVLGSCPGEGKV
Sbjct: 871 ASSTANKAILVGTSKEIRVLVEYMDDTDADSLKTAAEFLIDNLQDPAAIVLGSCPGEGKV 930

Query: 814 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 873
           SLVAAFTPTVVDLGVQAGKFIG IAKLCGGGGGGRPNFAQAGGRKPENLL+ALENARSEL
Sbjct: 931 SLVAAFTPTVVDLGVQAGKFIGSIAKLCGGGGGGRPNFAQAGGRKPENLLDALENARSEL 990

Query: 874 IHILSEKAS 883
           I ILSEKAS
Sbjct: 991 IRILSEKAS 999

BLAST of Sed0019949 vs. NCBI nr
Match: XP_022970912.1 (alanine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 1588.9 bits (4113), Expect = 0.0e+00
Identity = 795/849 (93.64%), Postives = 827/849 (97.41%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           VPRLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE
Sbjct: 145 VPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 204

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPANRLWISIYEEDDEAFAIWHDEVGVPV+RIKRMGE+DNFWTSGITGPCGPCSEIYY
Sbjct: 205 FGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCSEIYY 264

Query: 154 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 213
           DFQPEKG+SD+DLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ
Sbjct: 265 DFQPEKGHSDIDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 324

Query: 214 KVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNI 273
           KVPNNYETDLIYPIIQTASELA+V Y+TAD QSKT+LKIIGDHMRA+VYLISDGVVPSNI
Sbjct: 325 KVPNNYETDLIYPIIQTASELAHVTYETADAQSKTSLKIIGDHMRAIVYLISDGVVPSNI 384

Query: 274 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRI 333
           GRGY+VRRL+RRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALSSHIDEDVK R GRI
Sbjct: 385 GRGYIVRRLVRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVKGRTGRI 444

Query: 334 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEI 393
           LEELKREELRFVQTLERGEKLLEEML+DALASAKEDGR PCL GKDAFLLYDTYGFPVEI
Sbjct: 445 LEELKREELRFVQTLERGEKLLEEMLSDALASAKEDGRFPCLAGKDAFLLYDTYGFPVEI 504

Query: 394 TTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLL 453
           TTEVAQERGVVVDMKGFD EMDNQRRQSQAAHNAVKLEVGEGADIM NIS TEFLGYD+L
Sbjct: 505 TTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEFLGYDML 564

Query: 454 SAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVV 513
           SAKAIVESLIVNG SVLQVSEG+DVE+LLNRTPFYAESGGQIGDHGFIYISEGENR+NVV
Sbjct: 565 SAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGENRENVV 624

Query: 514 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 573
           +EVKDVQKSAGSIF+HKGTIKQGVLEVGREVEA VD KLRQGAKVHHTATHLLQSAL+R+
Sbjct: 625 MEVKDVQKSAGSIFVHKGTIKQGVLEVGREVEAAVDAKLRQGAKVHHTATHLLQSALRRI 684

Query: 574 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 633
           IGQETSQAGSLVAFDRLRFDF+YHRPLADTEIL+IEELINGWIGDAVLLQTKVMPL +AK
Sbjct: 685 IGQETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVMPLAEAK 744

Query: 634 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 693
            AGA+AMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA
Sbjct: 745 SAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 804

Query: 694 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 753
           VAGDAF+EY+NARDYHMKRLCTMLKVKAEDVTTRVENL E+LRLARNEISNLREKTAVFK
Sbjct: 805 VAGDAFVEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRLARNEISNLREKTAVFK 864

Query: 754 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 813
           ASS+ANKAFVVGTSKEIRVLVE MDDTDADSLK AAEFL++NLQDPAAIVLGSCPGEGKV
Sbjct: 865 ASSLANKAFVVGTSKEIRVLVEYMDDTDADSLKGAAEFLMNNLQDPAAIVLGSCPGEGKV 924

Query: 814 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 873
           SLVAAFTPTVVDLGVQA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL
Sbjct: 925 SLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 984

Query: 874 IHILSEKAS 883
           + ILSEKAS
Sbjct: 985 VRILSEKAS 993

BLAST of Sed0019949 vs. ExPASy Swiss-Prot
Match: B9HQZ6 (Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_821063 PE=3 SV=1)

HSP 1 Score: 1361.7 bits (3523), Expect = 0.0e+00
Identity = 669/848 (78.89%), Postives = 759/848 (89.50%), Query Frame = 0

Query: 35  PRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIEF 94
           PR VP A T+Q+CIRTNDVENVGRT RHHTFFEMLGNFSFGDYFKKEAIKWAWEL+T EF
Sbjct: 150 PRQVPRATTAQKCIRTNDVENVGRTTRHHTFFEMLGNFSFGDYFKKEAIKWAWELSTKEF 209

Query: 95  GLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYYD 154
           GLPA+RLW+S+YE+DDEAF IWHDEVGVPV+RIKRMGEEDNFWTSG TGPCGPCSE+YYD
Sbjct: 210 GLPADRLWVSVYEDDDEAFEIWHDEVGVPVERIKRMGEEDNFWTSGATGPCGPCSELYYD 269

Query: 155 FQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQK 214
           F PE+GY + DLGDD+RF+EFYNLVFMQYNK DDGSLEPLKQKNIDTGLGLER+ARILQK
Sbjct: 270 FHPERGYKNTDLGDDSRFIEFYNLVFMQYNKMDDGSLEPLKQKNIDTGLGLERLARILQK 329

Query: 215 VPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNIG 274
           VPNNYETDLIYPII+ A+ELAN+ Y  AD+++K NLKIIGDH+RA+VYLISDGV+PSNIG
Sbjct: 330 VPNNYETDLIYPIIEKAAELANISYALADDRTKMNLKIIGDHLRAIVYLISDGVLPSNIG 389

Query: 275 RGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRIL 334
           RGYVVRRLIRR VRTGRLLG+KG G    DG F P IAEKVI LS HID DVK+R   IL
Sbjct: 390 RGYVVRRLIRRAVRTGRLLGVKGGGE---DGVFLPAIAEKVIELSPHIDPDVKARGHSIL 449

Query: 335 EELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEIT 394
           +EL+REELRFVQTLERGEKLL++MLA+AL +A++   +PCL GKD FLLYDT+GFPVEIT
Sbjct: 450 DELQREELRFVQTLERGEKLLDQMLAEALLNAQKSETLPCLSGKDVFLLYDTFGFPVEIT 509

Query: 395 TEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLLS 454
           TEVA+E+GV +DM GF+ EM+NQRRQSQAAHN VKL V  G D+  N+  TEFLGYD LS
Sbjct: 510 TEVAEEQGVKIDMDGFEVEMENQRRQSQAAHNVVKLAVENGGDLAENVHDTEFLGYDTLS 569

Query: 455 AKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVVV 514
           A+A+VESL++NG SV+QVSEG++VE+LLN+TPFYAESGGQIGDHGF+Y+++ +++Q  VV
Sbjct: 570 ARAVVESLLLNGKSVIQVSEGSEVEVLLNKTPFYAESGGQIGDHGFLYVTQDQSKQTAVV 629

Query: 515 EVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRVI 574
           E+KDVQKS GS+F+HKGTI++GVLEVGREVEA VD KLRQ AKVHHTATHLLQSALK+VI
Sbjct: 630 EIKDVQKSLGSVFVHKGTIREGVLEVGREVEAAVDAKLRQRAKVHHTATHLLQSALKKVI 689

Query: 575 GQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAKR 634
           GQETSQAGSLVAFDRLRFDF++HRPL D+E+ +IE LINGWIGD  LLQTKVM L DAK 
Sbjct: 690 GQETSQAGSLVAFDRLRFDFNFHRPLHDSELEEIENLINGWIGDGTLLQTKVMSLTDAKE 749

Query: 635 AGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEAV 694
           AGA+AMFGEKYGE+VRVVEVPGVSMELCGGTHV NTSEIR FKIISEQGIASG+RRIEAV
Sbjct: 750 AGAIAMFGEKYGEQVRVVEVPGVSMELCGGTHVSNTSEIRAFKIISEQGIASGIRRIEAV 809

Query: 695 AGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFKA 754
           AG+AFIEYINARD  MK LC+ LKVKAE+VTTRV+NL EELR  RNE+S LR K AV+KA
Sbjct: 810 AGEAFIEYINARDSQMKLLCSTLKVKAEEVTTRVDNLLEELRTVRNEVSALRAKAAVYKA 869

Query: 755 SSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKVS 814
           S IA+KAF VGTSK IRVLVE MDD DAD+LK+AAE+L+D LQDPAAI+LGSCP EGKVS
Sbjct: 870 SMIASKAFSVGTSKTIRVLVESMDDFDADALKSAAEYLMDTLQDPAAIILGSCPDEGKVS 929

Query: 815 LVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSELI 874
           LVAAFTP VVD+G+QAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENL NALE AR++LI
Sbjct: 930 LVAAFTPGVVDIGIQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLTNALEKARTDLI 989

Query: 875 HILSEKAS 883
            IL+EKA+
Sbjct: 990 LILTEKAN 994

BLAST of Sed0019949 vs. ExPASy Swiss-Prot
Match: B8B4H5 (Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=OsI_22356 PE=3 SV=1)

HSP 1 Score: 1281.5 bits (3315), Expect = 0.0e+00
Identity = 629/849 (74.09%), Postives = 732/849 (86.22%), Query Frame = 0

Query: 35  PRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIEF 94
           PR VPCA TSQ+CIRTND+ENVGRT+RH TFFEMLGNFSFGDYFKKEAI WAWELTT EF
Sbjct: 151 PRRVPCATTSQKCIRTNDIENVGRTSRHQTFFEMLGNFSFGDYFKKEAITWAWELTTKEF 210

Query: 95  GLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYYD 154
           GLP  RLWIS++++DDEAF+IWH+EVGVP +RIKR+GE+DNFWTSG TGPCGPCSEIYYD
Sbjct: 211 GLPPERLWISVFQDDDEAFSIWHNEVGVPKERIKRLGEDDNFWTSGATGPCGPCSEIYYD 270

Query: 155 FQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQK 214
           F PE+G SD DLGDD+RF+EFYNLVFMQYNKKDDGSLEPLKQKNIDTG+GLERMARILQK
Sbjct: 271 FYPERGSSDADLGDDSRFIEFYNLVFMQYNKKDDGSLEPLKQKNIDTGMGLERMARILQK 330

Query: 215 VPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNIG 274
           VPNNYETDLI+PII+ A+ +A V Y TAD+  KTNLKIIGDHMRAVVYLISDGV+PSNIG
Sbjct: 331 VPNNYETDLIFPIIEKAASMALVSYTTADDAMKTNLKIIGDHMRAVVYLISDGVIPSNIG 390

Query: 275 RGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRIL 334
           RGYVVRRLIRRVVRTGRL+GI+GDG GN +GAF P +AE  I+LS+ ID DV+SR   IL
Sbjct: 391 RGYVVRRLIRRVVRTGRLIGIRGDGHGNSEGAFLPSLAEVAISLSTEIDPDVESRRKSIL 450

Query: 335 EELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEIT 394
            EL+REELRFVQTLERGEKLL+E+L +AL+SA  +G  PCL GKD FLLYDTYGFPVEIT
Sbjct: 451 GELQREELRFVQTLERGEKLLDELLDEALSSAGNNGGKPCLSGKDVFLLYDTYGFPVEIT 510

Query: 395 TEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLLS 454
            E+A ERGV+VDMKGFD EM+NQR+QSQAAHN VKL VG   +I+ +I  TEFLGYD LS
Sbjct: 511 AEIAGERGVIVDMKGFDMEMENQRKQSQAAHNVVKLSVGNETEIVKSIPDTEFLGYDSLS 570

Query: 455 AKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYI-SEGENRQNVV 514
           A A+V+ L+VNG SV  VSEG+DVEI L+RTPFYAESGGQ+GD+GF+Y+  E + +Q  V
Sbjct: 571 ATAVVKGLLVNGNSVNVVSEGSDVEIFLDRTPFYAESGGQVGDNGFLYVYGEEDAKQKAV 630

Query: 515 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 574
           +E+ DVQKS G+IF+HKGTIKQG +EVG+E++A VD KLRQGAK HHTATHLLQSALK +
Sbjct: 631 IEINDVQKSLGNIFVHKGTIKQGSVEVGKEIDAAVDAKLRQGAKAHHTATHLLQSALKSI 690

Query: 575 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 634
           IG ETSQAGSLVAFDRLRFDF++HRPL++ E++KIE L+N W+  A  L+TKVM L DAK
Sbjct: 691 IGSETSQAGSLVAFDRLRFDFNFHRPLSEEELMKIESLVNQWVSSATHLETKVMDLQDAK 750

Query: 635 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 694
            AGA+AMFGEKYGE+VRVVEVPGVSMELCGGTHV NT+EIRGFKIISEQGIASGVRRIEA
Sbjct: 751 NAGAIAMFGEKYGEQVRVVEVPGVSMELCGGTHVSNTAEIRGFKIISEQGIASGVRRIEA 810

Query: 695 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 754
           VAGDAF+EY+ ARD +M+ LC+ LKVKAEDV  RVE + EELR  RNE+S+LR K AV K
Sbjct: 811 VAGDAFVEYVCARDNYMRCLCSSLKVKAEDVNGRVETILEELRTTRNEVSSLRSKIAVLK 870

Query: 755 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 814
           A+S+ANKA  +  +   RV+VE M D DAD LK+AAE+L+D L+DPAA++LGS PG+GKV
Sbjct: 871 AASLANKATTIDNT---RVVVENMGDVDADGLKSAAEYLVDTLEDPAAVILGSSPGDGKV 930

Query: 815 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 874
           SLVAAF+P VV +G+QAGKF+G IAKLCGGGGGG+PNFAQAGGRKPENL  ALE AR E+
Sbjct: 931 SLVAAFSPGVVKMGIQAGKFVGGIAKLCGGGGGGKPNFAQAGGRKPENLPGALEKARDEI 990

Query: 875 IHILSEKAS 883
           +  +S K+S
Sbjct: 991 VAAISSKSS 996

BLAST of Sed0019949 vs. ExPASy Swiss-Prot
Match: B9FSH5 (Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0245800 PE=3 SV=2)

HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 628/849 (73.97%), Postives = 732/849 (86.22%), Query Frame = 0

Query: 35  PRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIEF 94
           PR VPCA TSQ+CIRTND+ENVGRT+RH TFFEMLGNFSFGDYFKKEAI WAWELTT EF
Sbjct: 151 PRRVPCATTSQKCIRTNDIENVGRTSRHQTFFEMLGNFSFGDYFKKEAITWAWELTTKEF 210

Query: 95  GLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYYD 154
           GLP  RLWIS++++DDEAF+IWH+EVGVP +RIKR+GE+DNFWTSG TGPCGPCSEIYYD
Sbjct: 211 GLPPERLWISVFQDDDEAFSIWHNEVGVPKERIKRLGEDDNFWTSGATGPCGPCSEIYYD 270

Query: 155 FQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQK 214
           F PE+G SD DLGDD+RF+EFYNLVFMQYNKKDDGSLEPLKQKNIDTG+GLERMARILQK
Sbjct: 271 FYPERGSSDADLGDDSRFIEFYNLVFMQYNKKDDGSLEPLKQKNIDTGMGLERMARILQK 330

Query: 215 VPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNIG 274
           VPNNYETDLI+PII+ A+ +A V Y TAD+  KTNLKIIGDHMRAVVYLISDGV+PSNIG
Sbjct: 331 VPNNYETDLIFPIIEKAASMALVSYTTADDAMKTNLKIIGDHMRAVVYLISDGVIPSNIG 390

Query: 275 RGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRIL 334
           RGYVVRRLIRRVVRTGRL+GI+GDG GN +GAF P +AE  I+LS+ ID DV+SR   IL
Sbjct: 391 RGYVVRRLIRRVVRTGRLIGIRGDGHGNSEGAFLPSLAEVAISLSTEIDPDVESRRKSIL 450

Query: 335 EELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEIT 394
            EL+REELRFVQTLERGEKLL+E+L +AL+SA  +G  PCL GKD FLLYDTYGFPVEIT
Sbjct: 451 GELQREELRFVQTLERGEKLLDELLDEALSSAGNNGGKPCLSGKDVFLLYDTYGFPVEIT 510

Query: 395 TEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLLS 454
            E++ ERGV+VDMKGFD EM+NQR+QSQAAHN VKL VG   +I+ +I  TEFLGYD LS
Sbjct: 511 AEISGERGVIVDMKGFDMEMENQRKQSQAAHNVVKLSVGNETEIVKSIPDTEFLGYDSLS 570

Query: 455 AKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYI-SEGENRQNVV 514
           A A+V+ L+VNG SV  VSEG+DVEI L+RTPFYAESGGQ+GD+GF+Y+  E + +Q  V
Sbjct: 571 ATAVVKGLLVNGNSVNVVSEGSDVEIFLDRTPFYAESGGQVGDNGFLYVYGEEDAKQKAV 630

Query: 515 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 574
           +E+ DVQKS G+IF+HKGTIKQG +EVG+E++A VD KLRQGAK HHTATHLLQSALK +
Sbjct: 631 IEINDVQKSLGNIFVHKGTIKQGSVEVGKEIDAAVDAKLRQGAKAHHTATHLLQSALKSI 690

Query: 575 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 634
           IG ETSQAGSLVAFDRLRFDF++HRPL++ E++KIE L+N W+  A  L+TKVM L DAK
Sbjct: 691 IGSETSQAGSLVAFDRLRFDFNFHRPLSEEELMKIESLVNQWVSSATHLETKVMDLQDAK 750

Query: 635 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 694
            AGA+AMFGEKYGE+VRVVEVPGVSMELCGGTHV NT+EIRGFKIISEQGIASGVRRIEA
Sbjct: 751 NAGAIAMFGEKYGEQVRVVEVPGVSMELCGGTHVSNTAEIRGFKIISEQGIASGVRRIEA 810

Query: 695 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 754
           VAGDAF+EY+ ARD +M+ LC+ LKVKAEDV  RVE + EELR  RNE+S+LR K AV K
Sbjct: 811 VAGDAFVEYVCARDNYMRCLCSSLKVKAEDVNGRVETILEELRTTRNEVSSLRSKIAVLK 870

Query: 755 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 814
           A+S+ANKA  +  +   RV+VE M D DAD LK+AAE+L+D L+DPAA++LGS PG+GKV
Sbjct: 871 AASLANKATTIDNT---RVVVENMGDVDADGLKSAAEYLVDTLEDPAAVILGSSPGDGKV 930

Query: 815 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 874
           SLVAAF+P VV +G+QAGKF+G IAKLCGGGGGG+PNFAQAGGRKPENL  ALE AR E+
Sbjct: 931 SLVAAFSPGVVKMGIQAGKFVGGIAKLCGGGGGGKPNFAQAGGRKPENLPGALEKARDEI 990

Query: 875 IHILSEKAS 883
           +  +S K+S
Sbjct: 991 VAAISSKSS 996

BLAST of Sed0019949 vs. ExPASy Swiss-Prot
Match: Q9FFC7 (Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=EMB86 PE=1 SV=2)

HSP 1 Score: 1278.1 bits (3306), Expect = 0.0e+00
Identity = 642/849 (75.62%), Postives = 733/849 (86.34%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           VPR VPCA T+QRCIRTND+ENVG+TARHHTFFEMLGNFSFGDYFKKEAIKWAWEL+TIE
Sbjct: 129 VPREVPCATTAQRCIRTNDLENVGKTARHHTFFEMLGNFSFGDYFKKEAIKWAWELSTIE 188

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPANR+W+SIYE+DDEAF IW +EVGV V+RIKRMGE DNFWTSG TGPCGPCSE+YY
Sbjct: 189 FGLPANRVWVSIYEDDDEAFEIWKNEVGVSVERIKRMGEADNFWTSGPTGPCGPCSELYY 248

Query: 154 DFQPEKGY-SDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARIL 213
           DF PE+GY  DVDLGDDTRF+EFYNLVFMQYNK +DG LEPLKQKNIDTGLGLER+A+IL
Sbjct: 249 DFYPERGYDEDVDLGDDTRFIEFYNLVFMQYNKTEDGLLEPLKQKNIDTGLGLERIAQIL 308

Query: 214 QKVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSN 273
           QKVPNNYETDLIYPII   SELAN+ YD+A++++KT+LK+I DHMRAVVYLISDGV PSN
Sbjct: 309 QKVPNNYETDLIYPIIAKISELANISYDSANDKAKTSLKVIADHMRAVVYLISDGVSPSN 368

Query: 274 IGRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGR 333
           IGRGYVVRRLIRR VR G+ LGI GD  GN+ GAF P +AEKVI LS++ID DVK +  R
Sbjct: 369 IGRGYVVRRLIRRAVRKGKSLGINGDMNGNLKGAFLPAVAEKVIELSTYIDSDVKLKASR 428

Query: 334 ILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVE 393
           I+EE+++EEL F +TLERGEKLL++ L DAL+ A +    P L GKDAFLLYDT+GFPVE
Sbjct: 429 IIEEIRQEELHFKKTLERGEKLLDQKLNDALSIADKTKDTPYLDGKDAFLLYDTFGFPVE 488

Query: 394 ITTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDL 453
           IT EVA+ERGV +DM GF+ EM+NQRRQSQAAHN VKL V + AD+  NI+ TEFLGYD 
Sbjct: 489 ITAEVAEERGVSIDMNGFEVEMENQRRQSQAAHNVVKLTVEDDADMTKNIADTEFLGYDS 548

Query: 454 LSAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNV 513
           LSA+A+V+SL+VNG  V++VSEG++VE+LL+RTPFYAESGGQI DHGF+Y+S   N++  
Sbjct: 549 LSARAVVKSLLVNGKPVIRVSEGSEVEVLLDRTPFYAESGGQIADHGFLYVSSDGNQEKA 608

Query: 514 VVEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKR 573
           VVEV DVQKS   IF+HKGT+K G LEVG+EVEA VD  LRQ AKVHHTATHLLQSALK+
Sbjct: 609 VVEVSDVQKSL-KIFVHKGTVKSGALEVGKEVEAAVDADLRQRAKVHHTATHLLQSALKK 668

Query: 574 VIGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDA 633
           V+GQETSQAGSLVAFDRLRFDF+++R L D E+ +IE LIN WIGDA  L+TKV+PL DA
Sbjct: 669 VVGQETSQAGSLVAFDRLRFDFNFNRSLHDNELEEIECLINRWIGDATRLETKVLPLADA 728

Query: 634 KRAGAVAMFGEKYGE-EVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRI 693
           KRAGA+AMFGEKY E EVRVVEVPGVSMELCGGTHV NT+EIR FKIISEQGIASG+RRI
Sbjct: 729 KRAGAIAMFGEKYDENEVRVVEVPGVSMELCGGTHVGNTAEIRAFKIISEQGIASGIRRI 788

Query: 694 EAVAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAV 753
           EAVAG+AFIEYIN+RD  M RLC+ LKVKAEDVT RVENL EELR AR E S+LR K AV
Sbjct: 789 EAVAGEAFIEYINSRDSQMTRLCSTLKVKAEDVTNRVENLLEELRAARKEASDLRSKAAV 848

Query: 754 FKASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEG 813
           +KAS I+NKAF VGTS+ IRVLVE MDDTDADSLK+AAE LI  L+DP A+VLGS P + 
Sbjct: 849 YKASVISNKAFTVGTSQTIRVLVESMDDTDADSLKSAAEHLISTLEDPVAVVLGSSPEKD 908

Query: 814 KVSLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARS 873
           KVSLVAAF+P VV LGVQAGKFIGPIAKLCGGGGGG+PNFAQAGGRKPENL +ALE AR 
Sbjct: 909 KVSLVAAFSPGVVSLGVQAGKFIGPIAKLCGGGGGGKPNFAQAGGRKPENLPSALEKARE 968

Query: 874 ELIHILSEK 881
           +L+  LSEK
Sbjct: 969 DLVATLSEK 976

BLAST of Sed0019949 vs. ExPASy Swiss-Prot
Match: C5Z7K4 (Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Sorghum bicolor OX=4558 GN=Sb10g008780 PE=3 SV=1)

HSP 1 Score: 1253.0 bits (3241), Expect = 0.0e+00
Identity = 618/841 (73.48%), Postives = 723/841 (85.97%), Query Frame = 0

Query: 35  PRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIEF 94
           PR VPCA TSQ+CIRTND+ENVGRTARH TFFEMLGNFSFGDYFKKEA  WAWEL T E+
Sbjct: 115 PRRVPCATTSQKCIRTNDIENVGRTARHQTFFEMLGNFSFGDYFKKEATAWAWELATKEY 174

Query: 95  GLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYYD 154
           GLPA RLWIS++E+D+EAF IWH+EVGVP +RIKRMG EDNFWTSG TGPCGPCSEIYYD
Sbjct: 175 GLPAERLWISVFEDDNEAFDIWHNEVGVPKERIKRMGAEDNFWTSGATGPCGPCSEIYYD 234

Query: 155 FQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQK 214
           F PE+G SD DLGDD+RF+EFYNLVFMQYNKKDDGSLEPLKQKNIDTG+GLERMARILQK
Sbjct: 235 FYPERGSSDADLGDDSRFIEFYNLVFMQYNKKDDGSLEPLKQKNIDTGMGLERMARILQK 294

Query: 215 VPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNIG 274
           VPNNYETDLI+PII+ A+ +A V Y   D+ +KTNLKIIGDHMRAVVYL+SDGV+PSNIG
Sbjct: 295 VPNNYETDLIFPIIEKAASMAMVSYAKTDDATKTNLKIIGDHMRAVVYLVSDGVIPSNIG 354

Query: 275 RGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRIL 334
           RGYVVRRLIRRVVR GRL+GI+GDG GN +GAF P +AE VI+LS+ ID DV+SR   IL
Sbjct: 355 RGYVVRRLIRRVVRMGRLIGIRGDGHGNSEGAFLPSLAEVVISLSTEIDPDVESRRRSIL 414

Query: 335 EELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEIT 394
            EL+REELRFVQTLERGEKLL+E+L  A+ SA  +G  P L GKD FLLYDTYGFPVEIT
Sbjct: 415 GELQREELRFVQTLERGEKLLDELLDGAVLSAGNNGDKPSLSGKDLFLLYDTYGFPVEIT 474

Query: 395 TEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLLS 454
            E+A ERGV VD++GFD EM+NQR+QSQAAHN VKL VG   +I+ +I  T FLGYD LS
Sbjct: 475 AEIASERGVTVDIEGFDIEMENQRKQSQAAHNVVKLSVGNETEIIKSIPDTVFLGYDSLS 534

Query: 455 AKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENR-QNVV 514
           A A+V  L+VNG  V +VSEG++VEILL+RTPFYAESGGQ+GD+GF+Y++ GE+R Q  V
Sbjct: 535 ASAVVRGLLVNGNPVNEVSEGSEVEILLDRTPFYAESGGQVGDNGFLYVNGGEDRSQAAV 594

Query: 515 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 574
           +E+ DVQKS G+IF+HKGTIKQG +EVG+EV+A VD KLRQGAK HHTATHLLQSALK V
Sbjct: 595 IEINDVQKSLGNIFVHKGTIKQGSVEVGKEVDACVDAKLRQGAKAHHTATHLLQSALKSV 654

Query: 575 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 634
           +G ETSQAGSLVAFDRLRFDF++HRP+++ E+ +IE L+N WI +A  L+TKVM L DAK
Sbjct: 655 VGSETSQAGSLVAFDRLRFDFNFHRPVSEGELTRIELLVNQWISNAAHLETKVMALQDAK 714

Query: 635 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 694
            AGA+AMFGEKYGE+VRVVEVPGVS+ELCGGTHV NT+EIRGFKIISEQGIASG+RRIEA
Sbjct: 715 NAGAIAMFGEKYGEQVRVVEVPGVSLELCGGTHVSNTAEIRGFKIISEQGIASGIRRIEA 774

Query: 695 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 754
           VAGDAFI+Y+ ARD +M+RLC+ LKVKAEDV  RVE + EELR  RNE+S+LR K AV K
Sbjct: 775 VAGDAFIDYVCARDNYMRRLCSSLKVKAEDVNGRVETILEELRATRNEVSSLRSKIAVLK 834

Query: 755 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 814
           A+S+A+KA  V   + +R++VE M D DAD LK+AAE+LI  LQDPAA++LGS PG+GKV
Sbjct: 835 AASLASKATTV-EPQNVRIVVENMGDVDADGLKSAAEYLIGTLQDPAAVILGSSPGDGKV 894

Query: 815 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 874
           SLVAAF+P VV +G+QAGKF+G IAKLCGGGGGG+PNFAQAGGRKPENL +ALE AR+E+
Sbjct: 895 SLVAAFSPAVVKMGLQAGKFVGGIAKLCGGGGGGKPNFAQAGGRKPENLPDALEKARAEI 954

BLAST of Sed0019949 vs. ExPASy TrEMBL
Match: A0A6J1I0H1 (Probable alanine--tRNA ligase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111469744 PE=3 SV=1)

HSP 1 Score: 1588.9 bits (4113), Expect = 0.0e+00
Identity = 795/849 (93.64%), Postives = 827/849 (97.41%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           VPRLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE
Sbjct: 145 VPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 204

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPANRLWISIYEEDDEAFAIWHDEVGVPV+RIKRMGE+DNFWTSGITGPCGPCSEIYY
Sbjct: 205 FGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCSEIYY 264

Query: 154 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 213
           DFQPEKG+SD+DLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ
Sbjct: 265 DFQPEKGHSDIDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 324

Query: 214 KVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNI 273
           KVPNNYETDLIYPIIQTASELA+V Y+TAD QSKT+LKIIGDHMRA+VYLISDGVVPSNI
Sbjct: 325 KVPNNYETDLIYPIIQTASELAHVTYETADAQSKTSLKIIGDHMRAIVYLISDGVVPSNI 384

Query: 274 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRI 333
           GRGY+VRRL+RRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALSSHIDEDVK R GRI
Sbjct: 385 GRGYIVRRLVRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVKGRTGRI 444

Query: 334 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEI 393
           LEELKREELRFVQTLERGEKLLEEML+DALASAKEDGR PCL GKDAFLLYDTYGFPVEI
Sbjct: 445 LEELKREELRFVQTLERGEKLLEEMLSDALASAKEDGRFPCLAGKDAFLLYDTYGFPVEI 504

Query: 394 TTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLL 453
           TTEVAQERGVVVDMKGFD EMDNQRRQSQAAHNAVKLEVGEGADIM NIS TEFLGYD+L
Sbjct: 505 TTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEFLGYDML 564

Query: 454 SAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVV 513
           SAKAIVESLIVNG SVLQVSEG+DVE+LLNRTPFYAESGGQIGDHGFIYISEGENR+NVV
Sbjct: 565 SAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGENRENVV 624

Query: 514 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 573
           +EVKDVQKSAGSIF+HKGTIKQGVLEVGREVEA VD KLRQGAKVHHTATHLLQSAL+R+
Sbjct: 625 MEVKDVQKSAGSIFVHKGTIKQGVLEVGREVEAAVDAKLRQGAKVHHTATHLLQSALRRI 684

Query: 574 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 633
           IGQETSQAGSLVAFDRLRFDF+YHRPLADTEIL+IEELINGWIGDAVLLQTKVMPL +AK
Sbjct: 685 IGQETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVMPLAEAK 744

Query: 634 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 693
            AGA+AMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA
Sbjct: 745 SAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 804

Query: 694 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 753
           VAGDAF+EY+NARDYHMKRLCTMLKVKAEDVTTRVENL E+LRLARNEISNLREKTAVFK
Sbjct: 805 VAGDAFVEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRLARNEISNLREKTAVFK 864

Query: 754 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 813
           ASS+ANKAFVVGTSKEIRVLVE MDDTDADSLK AAEFL++NLQDPAAIVLGSCPGEGKV
Sbjct: 865 ASSLANKAFVVGTSKEIRVLVEYMDDTDADSLKGAAEFLMNNLQDPAAIVLGSCPGEGKV 924

Query: 814 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 873
           SLVAAFTPTVVDLGVQA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL
Sbjct: 925 SLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 984

Query: 874 IHILSEKAS 883
           + ILSEKAS
Sbjct: 985 VRILSEKAS 993

BLAST of Sed0019949 vs. ExPASy TrEMBL
Match: A0A6J1I5A9 (Probable alanine--tRNA ligase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111469744 PE=3 SV=1)

HSP 1 Score: 1588.9 bits (4113), Expect = 0.0e+00
Identity = 795/849 (93.64%), Postives = 827/849 (97.41%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           VPRLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE
Sbjct: 91  VPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 150

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPANRLWISIYEEDDEAFAIWHDEVGVPV+RIKRMGE+DNFWTSGITGPCGPCSEIYY
Sbjct: 151 FGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCSEIYY 210

Query: 154 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 213
           DFQPEKG+SD+DLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ
Sbjct: 211 DFQPEKGHSDIDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 270

Query: 214 KVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNI 273
           KVPNNYETDLIYPIIQTASELA+V Y+TAD QSKT+LKIIGDHMRA+VYLISDGVVPSNI
Sbjct: 271 KVPNNYETDLIYPIIQTASELAHVTYETADAQSKTSLKIIGDHMRAIVYLISDGVVPSNI 330

Query: 274 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRI 333
           GRGY+VRRL+RRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALSSHIDEDVK R GRI
Sbjct: 331 GRGYIVRRLVRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVKGRTGRI 390

Query: 334 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEI 393
           LEELKREELRFVQTLERGEKLLEEML+DALASAKEDGR PCL GKDAFLLYDTYGFPVEI
Sbjct: 391 LEELKREELRFVQTLERGEKLLEEMLSDALASAKEDGRFPCLAGKDAFLLYDTYGFPVEI 450

Query: 394 TTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLL 453
           TTEVAQERGVVVDMKGFD EMDNQRRQSQAAHNAVKLEVGEGADIM NIS TEFLGYD+L
Sbjct: 451 TTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEFLGYDML 510

Query: 454 SAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVV 513
           SAKAIVESLIVNG SVLQVSEG+DVE+LLNRTPFYAESGGQIGDHGFIYISEGENR+NVV
Sbjct: 511 SAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGENRENVV 570

Query: 514 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 573
           +EVKDVQKSAGSIF+HKGTIKQGVLEVGREVEA VD KLRQGAKVHHTATHLLQSAL+R+
Sbjct: 571 MEVKDVQKSAGSIFVHKGTIKQGVLEVGREVEAAVDAKLRQGAKVHHTATHLLQSALRRI 630

Query: 574 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 633
           IGQETSQAGSLVAFDRLRFDF+YHRPLADTEIL+IEELINGWIGDAVLLQTKVMPL +AK
Sbjct: 631 IGQETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVMPLAEAK 690

Query: 634 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 693
            AGA+AMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA
Sbjct: 691 SAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 750

Query: 694 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 753
           VAGDAF+EY+NARDYHMKRLCTMLKVKAEDVTTRVENL E+LRLARNEISNLREKTAVFK
Sbjct: 751 VAGDAFVEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRLARNEISNLREKTAVFK 810

Query: 754 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 813
           ASS+ANKAFVVGTSKEIRVLVE MDDTDADSLK AAEFL++NLQDPAAIVLGSCPGEGKV
Sbjct: 811 ASSLANKAFVVGTSKEIRVLVEYMDDTDADSLKGAAEFLMNNLQDPAAIVLGSCPGEGKV 870

Query: 814 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 873
           SLVAAFTPTVVDLGVQA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL
Sbjct: 871 SLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 930

Query: 874 IHILSEKAS 883
           + ILSEKAS
Sbjct: 931 VRILSEKAS 939

BLAST of Sed0019949 vs. ExPASy TrEMBL
Match: A0A6J1I1V0 (Probable alanine--tRNA ligase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111469744 PE=3 SV=1)

HSP 1 Score: 1588.9 bits (4113), Expect = 0.0e+00
Identity = 795/849 (93.64%), Postives = 827/849 (97.41%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           VPRLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE
Sbjct: 151 VPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 210

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPANRLWISIYEEDDEAFAIWHDEVGVPV+RIKRMGE+DNFWTSGITGPCGPCSEIYY
Sbjct: 211 FGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCSEIYY 270

Query: 154 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 213
           DFQPEKG+SD+DLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ
Sbjct: 271 DFQPEKGHSDIDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 330

Query: 214 KVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNI 273
           KVPNNYETDLIYPIIQTASELA+V Y+TAD QSKT+LKIIGDHMRA+VYLISDGVVPSNI
Sbjct: 331 KVPNNYETDLIYPIIQTASELAHVTYETADAQSKTSLKIIGDHMRAIVYLISDGVVPSNI 390

Query: 274 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRI 333
           GRGY+VRRL+RRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALSSHIDEDVK R GRI
Sbjct: 391 GRGYIVRRLVRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVKGRTGRI 450

Query: 334 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEI 393
           LEELKREELRFVQTLERGEKLLEEML+DALASAKEDGR PCL GKDAFLLYDTYGFPVEI
Sbjct: 451 LEELKREELRFVQTLERGEKLLEEMLSDALASAKEDGRFPCLAGKDAFLLYDTYGFPVEI 510

Query: 394 TTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLL 453
           TTEVAQERGVVVDMKGFD EMDNQRRQSQAAHNAVKLEVGEGADIM NIS TEFLGYD+L
Sbjct: 511 TTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEFLGYDML 570

Query: 454 SAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVV 513
           SAKAIVESLIVNG SVLQVSEG+DVE+LLNRTPFYAESGGQIGDHGFIYISEGENR+NVV
Sbjct: 571 SAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGENRENVV 630

Query: 514 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 573
           +EVKDVQKSAGSIF+HKGTIKQGVLEVGREVEA VD KLRQGAKVHHTATHLLQSAL+R+
Sbjct: 631 MEVKDVQKSAGSIFVHKGTIKQGVLEVGREVEAAVDAKLRQGAKVHHTATHLLQSALRRI 690

Query: 574 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 633
           IGQETSQAGSLVAFDRLRFDF+YHRPLADTEIL+IEELINGWIGDAVLLQTKVMPL +AK
Sbjct: 691 IGQETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVMPLAEAK 750

Query: 634 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 693
            AGA+AMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA
Sbjct: 751 SAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 810

Query: 694 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 753
           VAGDAF+EY+NARDYHMKRLCTMLKVKAEDVTTRVENL E+LRLARNEISNLREKTAVFK
Sbjct: 811 VAGDAFVEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRLARNEISNLREKTAVFK 870

Query: 754 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 813
           ASS+ANKAFVVGTSKEIRVLVE MDDTDADSLK AAEFL++NLQDPAAIVLGSCPGEGKV
Sbjct: 871 ASSLANKAFVVGTSKEIRVLVEYMDDTDADSLKGAAEFLMNNLQDPAAIVLGSCPGEGKV 930

Query: 814 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 873
           SLVAAFTPTVVDLGVQA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL
Sbjct: 931 SLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 990

Query: 874 IHILSEKAS 883
           + ILSEKAS
Sbjct: 991 VRILSEKAS 999

BLAST of Sed0019949 vs. ExPASy TrEMBL
Match: A0A6J1G7M4 (Probable alanine--tRNA ligase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451483 PE=3 SV=1)

HSP 1 Score: 1583.9 bits (4100), Expect = 0.0e+00
Identity = 795/849 (93.64%), Postives = 825/849 (97.17%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           V RLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFK+EAIKWAWELTTIE
Sbjct: 145 VSRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKREAIKWAWELTTIE 204

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPAN+LWISIYEEDDEAFAIWHDEVGVPV+RIKRMGE+DNFWTSGITGPCGPCSEIYY
Sbjct: 205 FGLPANKLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCSEIYY 264

Query: 154 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 213
           DFQPEKG+SDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ
Sbjct: 265 DFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 324

Query: 214 KVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNI 273
           KVPNNYETDLIYPIIQ ASELA+V Y+TAD QSKT+LKIIGDHMRA+VYLISDGVVPSNI
Sbjct: 325 KVPNNYETDLIYPIIQKASELAHVTYETADAQSKTSLKIIGDHMRAIVYLISDGVVPSNI 384

Query: 274 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRI 333
           GRGY+VRRLIRRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALSSHIDEDVK R GRI
Sbjct: 385 GRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVKGRTGRI 444

Query: 334 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEI 393
           LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGR PCL GKDAFLLYDTYGFPVEI
Sbjct: 445 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLYDTYGFPVEI 504

Query: 394 TTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLL 453
           TTEVAQERGVVVDMKGFD EMDNQRRQSQAAHNAVKLEVGEGADIM NIS TEFLGYD+L
Sbjct: 505 TTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEFLGYDML 564

Query: 454 SAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVV 513
           SAKAIVESLIVNG SVLQVSEG+DVE+LLNRTPFYAESGGQIGDHGFIYISEGENR NVV
Sbjct: 565 SAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGENRGNVV 624

Query: 514 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 573
           +EVKDVQKSAGSIF+HKGTIKQGVLEVGREVEAEVD +LRQGAKVHHTATHLLQSAL+R+
Sbjct: 625 MEVKDVQKSAGSIFVHKGTIKQGVLEVGREVEAEVDARLRQGAKVHHTATHLLQSALRRI 684

Query: 574 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 633
           IGQETSQAGSLVAFDRLRFDF+YHRPLADTEIL+IEELINGWIGDAVLLQTKVMPL +AK
Sbjct: 685 IGQETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVMPLAEAK 744

Query: 634 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 693
            AGA+AMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFK+ISEQGIASGVRRIEA
Sbjct: 745 SAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGIASGVRRIEA 804

Query: 694 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 753
           VAGDAFIEY+NARDYHMKRLCTMLKVKAEDVTTRVENL E+LRLARNEISNLREKTAVFK
Sbjct: 805 VAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRLARNEISNLREKTAVFK 864

Query: 754 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 813
           ASSIANKAFVVGTSKEIRVLVE MDDTDADSLK AAEFL+DNLQDPAAIVLGSCPGEGKV
Sbjct: 865 ASSIANKAFVVGTSKEIRVLVEYMDDTDADSLKGAAEFLMDNLQDPAAIVLGSCPGEGKV 924

Query: 814 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 873
           SLVAAFTP+VVDLGVQA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL
Sbjct: 925 SLVAAFTPSVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 984

Query: 874 IHILSEKAS 883
           + ILSEKAS
Sbjct: 985 VRILSEKAS 993

BLAST of Sed0019949 vs. ExPASy TrEMBL
Match: A0A6J1G7K6 (Probable alanine--tRNA ligase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451483 PE=3 SV=1)

HSP 1 Score: 1583.9 bits (4100), Expect = 0.0e+00
Identity = 795/849 (93.64%), Postives = 825/849 (97.17%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           V RLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFK+EAIKWAWELTTIE
Sbjct: 151 VSRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKREAIKWAWELTTIE 210

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPAN+LWISIYEEDDEAFAIWHDEVGVPV+RIKRMGE+DNFWTSGITGPCGPCSEIYY
Sbjct: 211 FGLPANKLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCSEIYY 270

Query: 154 DFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 213
           DFQPEKG+SDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ
Sbjct: 271 DFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQ 330

Query: 214 KVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSNI 273
           KVPNNYETDLIYPIIQ ASELA+V Y+TAD QSKT+LKIIGDHMRA+VYLISDGVVPSNI
Sbjct: 331 KVPNNYETDLIYPIIQKASELAHVTYETADAQSKTSLKIIGDHMRAIVYLISDGVVPSNI 390

Query: 274 GRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGRI 333
           GRGY+VRRLIRRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALSSHIDEDVK R GRI
Sbjct: 391 GRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVKGRTGRI 450

Query: 334 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVEI 393
           LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGR PCL GKDAFLLYDTYGFPVEI
Sbjct: 451 LEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLYDTYGFPVEI 510

Query: 394 TTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDLL 453
           TTEVAQERGVVVDMKGFD EMDNQRRQSQAAHNAVKLEVGEGADIM NIS TEFLGYD+L
Sbjct: 511 TTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEFLGYDML 570

Query: 454 SAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNVV 513
           SAKAIVESLIVNG SVLQVSEG+DVE+LLNRTPFYAESGGQIGDHGFIYISEGENR NVV
Sbjct: 571 SAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGENRGNVV 630

Query: 514 VEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKRV 573
           +EVKDVQKSAGSIF+HKGTIKQGVLEVGREVEAEVD +LRQGAKVHHTATHLLQSAL+R+
Sbjct: 631 MEVKDVQKSAGSIFVHKGTIKQGVLEVGREVEAEVDARLRQGAKVHHTATHLLQSALRRI 690

Query: 574 IGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDAK 633
           IGQETSQAGSLVAFDRLRFDF+YHRPLADTEIL+IEELINGWIGDAVLLQTKVMPL +AK
Sbjct: 691 IGQETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVMPLAEAK 750

Query: 634 RAGAVAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEA 693
            AGA+AMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFK+ISEQGIASGVRRIEA
Sbjct: 751 SAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGIASGVRRIEA 810

Query: 694 VAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFK 753
           VAGDAFIEY+NARDYHMKRLCTMLKVKAEDVTTRVENL E+LRLARNEISNLREKTAVFK
Sbjct: 811 VAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRLARNEISNLREKTAVFK 870

Query: 754 ASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKV 813
           ASSIANKAFVVGTSKEIRVLVE MDDTDADSLK AAEFL+DNLQDPAAIVLGSCPGEGKV
Sbjct: 871 ASSIANKAFVVGTSKEIRVLVEYMDDTDADSLKGAAEFLMDNLQDPAAIVLGSCPGEGKV 930

Query: 814 SLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 873
           SLVAAFTP+VVDLGVQA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL
Sbjct: 931 SLVAAFTPSVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARSEL 990

Query: 874 IHILSEKAS 883
           + ILSEKAS
Sbjct: 991 VRILSEKAS 999

BLAST of Sed0019949 vs. TAIR 10
Match: AT5G22800.1 (Alanyl-tRNA synthetase, class IIc )

HSP 1 Score: 1278.1 bits (3306), Expect = 0.0e+00
Identity = 642/849 (75.62%), Postives = 733/849 (86.34%), Query Frame = 0

Query: 34  VPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIE 93
           VPR VPCA T+QRCIRTND+ENVG+TARHHTFFEMLGNFSFGDYFKKEAIKWAWEL+TIE
Sbjct: 129 VPREVPCATTAQRCIRTNDLENVGKTARHHTFFEMLGNFSFGDYFKKEAIKWAWELSTIE 188

Query: 94  FGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPCSEIYY 153
           FGLPANR+W+SIYE+DDEAF IW +EVGV V+RIKRMGE DNFWTSG TGPCGPCSE+YY
Sbjct: 189 FGLPANRVWVSIYEDDDEAFEIWKNEVGVSVERIKRMGEADNFWTSGPTGPCGPCSELYY 248

Query: 154 DFQPEKGY-SDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARIL 213
           DF PE+GY  DVDLGDDTRF+EFYNLVFMQYNK +DG LEPLKQKNIDTGLGLER+A+IL
Sbjct: 249 DFYPERGYDEDVDLGDDTRFIEFYNLVFMQYNKTEDGLLEPLKQKNIDTGLGLERIAQIL 308

Query: 214 QKVPNNYETDLIYPIIQTASELANVIYDTADNQSKTNLKIIGDHMRAVVYLISDGVVPSN 273
           QKVPNNYETDLIYPII   SELAN+ YD+A++++KT+LK+I DHMRAVVYLISDGV PSN
Sbjct: 309 QKVPNNYETDLIYPIIAKISELANISYDSANDKAKTSLKVIADHMRAVVYLISDGVSPSN 368

Query: 274 IGRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHIDEDVKSREGR 333
           IGRGYVVRRLIRR VR G+ LGI GD  GN+ GAF P +AEKVI LS++ID DVK +  R
Sbjct: 369 IGRGYVVRRLIRRAVRKGKSLGINGDMNGNLKGAFLPAVAEKVIELSTYIDSDVKLKASR 428

Query: 334 ILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFLLYDTYGFPVE 393
           I+EE+++EEL F +TLERGEKLL++ L DAL+ A +    P L GKDAFLLYDT+GFPVE
Sbjct: 429 IIEEIRQEELHFKKTLERGEKLLDQKLNDALSIADKTKDTPYLDGKDAFLLYDTFGFPVE 488

Query: 394 ITTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHNAVKLEVGEGADIMGNISHTEFLGYDL 453
           IT EVA+ERGV +DM GF+ EM+NQRRQSQAAHN VKL V + AD+  NI+ TEFLGYD 
Sbjct: 489 ITAEVAEERGVSIDMNGFEVEMENQRRQSQAAHNVVKLTVEDDADMTKNIADTEFLGYDS 548

Query: 454 LSAKAIVESLIVNGTSVLQVSEGNDVEILLNRTPFYAESGGQIGDHGFIYISEGENRQNV 513
           LSA+A+V+SL+VNG  V++VSEG++VE+LL+RTPFYAESGGQI DHGF+Y+S   N++  
Sbjct: 549 LSARAVVKSLLVNGKPVIRVSEGSEVEVLLDRTPFYAESGGQIADHGFLYVSSDGNQEKA 608

Query: 514 VVEVKDVQKSAGSIFIHKGTIKQGVLEVGREVEAEVDTKLRQGAKVHHTATHLLQSALKR 573
           VVEV DVQKS   IF+HKGT+K G LEVG+EVEA VD  LRQ AKVHHTATHLLQSALK+
Sbjct: 609 VVEVSDVQKSL-KIFVHKGTVKSGALEVGKEVEAAVDADLRQRAKVHHTATHLLQSALKK 668

Query: 574 VIGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELINGWIGDAVLLQTKVMPLVDA 633
           V+GQETSQAGSLVAFDRLRFDF+++R L D E+ +IE LIN WIGDA  L+TKV+PL DA
Sbjct: 669 VVGQETSQAGSLVAFDRLRFDFNFNRSLHDNELEEIECLINRWIGDATRLETKVLPLADA 728

Query: 634 KRAGAVAMFGEKYGE-EVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRI 693
           KRAGA+AMFGEKY E EVRVVEVPGVSMELCGGTHV NT+EIR FKIISEQGIASG+RRI
Sbjct: 729 KRAGAIAMFGEKYDENEVRVVEVPGVSMELCGGTHVGNTAEIRAFKIISEQGIASGIRRI 788

Query: 694 EAVAGDAFIEYINARDYHMKRLCTMLKVKAEDVTTRVENLAEELRLARNEISNLREKTAV 753
           EAVAG+AFIEYIN+RD  M RLC+ LKVKAEDVT RVENL EELR AR E S+LR K AV
Sbjct: 789 EAVAGEAFIEYINSRDSQMTRLCSTLKVKAEDVTNRVENLLEELRAARKEASDLRSKAAV 848

Query: 754 FKASSIANKAFVVGTSKEIRVLVECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEG 813
           +KAS I+NKAF VGTS+ IRVLVE MDDTDADSLK+AAE LI  L+DP A+VLGS P + 
Sbjct: 849 YKASVISNKAFTVGTSQTIRVLVESMDDTDADSLKSAAEHLISTLEDPVAVVLGSSPEKD 908

Query: 814 KVSLVAAFTPTVVDLGVQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENARS 873
           KVSLVAAF+P VV LGVQAGKFIGPIAKLCGGGGGG+PNFAQAGGRKPENL +ALE AR 
Sbjct: 909 KVSLVAAFSPGVVSLGVQAGKFIGPIAKLCGGGGGGKPNFAQAGGRKPENLPSALEKARE 968

Query: 874 ELIHILSEK 881
           +L+  LSEK
Sbjct: 969 DLVATLSEK 976

BLAST of Sed0019949 vs. TAIR 10
Match: AT1G50200.1 (Alanyl-tRNA synthetase )

HSP 1 Score: 424.5 bits (1090), Expect = 2.1e-118
Identity = 285/846 (33.69%), Postives = 451/846 (53.31%), Query Frame = 0

Query: 41  AATSQRCIRT----NDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIEFGL 100
           A  +Q+CIR     ND+++VG+   HHTFFEMLGN+SFGDYFKKEAI+WAWEL T  +GL
Sbjct: 123 ACNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKKEAIEWAWELLTKVYGL 182

Query: 101 PANRLWISIY--------EEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPC 160
           P +R++ + +        + D+EA  IW     +P  R+   G +DNFW  G TGPCGPC
Sbjct: 183 PTDRIYATYFGGDEKAGLQPDNEARDIWLKV--LPSGRVLPFGCKDNFWEMGDTGPCGPC 242

Query: 161 SEIYYDFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERM 220
           +EI+YD    +  + +   DD   +E +NLVF+Q+N++ DGSL+PL  K++DTG+G ER+
Sbjct: 243 TEIHYDRIGNRDAASLVNNDDPTCLEIWNLVFIQFNRESDGSLKPLPAKHVDTGMGFERL 302

Query: 221 ARILQKVPNNYETDLIYPIIQT------ASELANVIYDTADNQSKTNLKIIGDHMRAVVY 280
             +LQ   +NY+TD+  PI         A   +  +     ++     +++ DH+R + +
Sbjct: 303 TSVLQNKMSNYDTDVFMPIFDDIQKATGARPYSGKVGPEDVDRVDMAYRVVADHIRTLSF 362

Query: 281 LISDGVVPSNIGRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHI 340
            I+DG  P N GR YV+RR++RR VR G+ + +K +     +G F  L++  VI +   +
Sbjct: 363 AIADGSRPGNEGREYVLRRILRRAVRYGKEI-LKAE-----EGFFNGLVS-SVIRVMGDV 422

Query: 341 DEDVKSREGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFL 400
             ++K  E +I + +K EE  F +TL +G         +    A +  +   L G DAF+
Sbjct: 423 FTELKEHEKKITDIIKEEEASFCKTLAKG--------IEKFRKAGQAVQGNTLSGDDAFI 482

Query: 401 LYDTYGFPVEITTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHN-----AVKLEVGEGAD 460
           L+DTYGFP+++T  +A+ERG++VD+ GF+  M+  R +S++A N     A+ ++    + 
Sbjct: 483 LWDTYGFPLDLTQLMAEERGLLVDVDGFNKAMEEARERSRSAQNKQAGGAIVMDADATST 542

Query: 461 I--MGNISHTEFLGYDLLSAKAIVESLIVNGTSVLQVSEGND-VEILLNRTPFYAESGGQ 520
           +   G  +  +   Y            I  G++ L+ S  +D V ++L  T FYAE GGQ
Sbjct: 543 LHKAGVSATDDSFKYIWFQDHESELKAIYTGSTFLESSAASDNVGLVLGSTSFYAEQGGQ 602

Query: 521 IGDHGFIYISEGENRQNVVVEVKDVQKSAGSIFIHKGTIKQ--GVLEVGREVEAEVDTKL 580
           I D G I  S G         V +VQ   G + +H G + +  G + VG +V  +VD + 
Sbjct: 603 IFDTGLIEGSFG------TFNVCNVQIFGGFV-LHIGYLSKETGEVSVGDKVICKVDYER 662

Query: 581 RQGAKVHHTATHLLQSALKRVIGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELI 640
           R+    +HT TH+L  ALK V+G    Q GS+V  ++LRFDFS+ +P+   ++ +IE ++
Sbjct: 663 RKLIAPNHTCTHMLNYALKEVLGDHIDQKGSIVLPEKLRFDFSHGKPVDPEDLRRIESIV 722

Query: 641 NGWIGDAVLLQTKVMPLVDAKR-AGAVAMFGEKYGEEVRVVEVPG--------------- 700
           N  I D + + +K   L +AKR  G  A+FGE Y + VRVV +                 
Sbjct: 723 NKQIKDELDVFSKEAVLSEAKRIKGLRAVFGEVYPDPVRVVSIGRKVEDLLADPENNEWS 782

Query: 701 -VSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEAVAGDAFIEYINARDYHMKRLCT 760
            +S E CGGTH+ NT E + F ++SE+GIA G+RR+ AV  +   + +NA         +
Sbjct: 783 LLSSEFCGGTHITNTREAKAFALLSEEGIAKGIRRVTAVTTECAFDALNA--------AS 842

Query: 761 MLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFKASSIANKAFVVGTSK-EIRVLV 820
           +L+ + ED              +R E S L +K +  K  S  + A +    K +IR  +
Sbjct: 843 LLEREVEDA-------------SRAEGSALEKKVSALK--SRVDAAIIPAAKKADIRTKI 902

Query: 821 ECMDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKVSLVAAFTPTVVDLGVQAGKFI 841
             + +    + K  AE    NL+   ++ L +   E   S    F    +D+G+ A    
Sbjct: 903 ASLQNEVRKAQKKIAE---QNLK--KSVKLATEAAESAASDGKTFCIIQLDVGLDAAAVR 916

BLAST of Sed0019949 vs. TAIR 10
Match: AT1G50200.2 (Alanyl-tRNA synthetase )

HSP 1 Score: 424.1 bits (1089), Expect = 2.8e-118
Identity = 286/851 (33.61%), Postives = 452/851 (53.11%), Query Frame = 0

Query: 41  AATSQRCIRT----NDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIEFGL 100
           A  +Q+CIR     ND+++VG+   HHTFFEMLGN+SFGDYFKKEAI+WAWEL T  +GL
Sbjct: 75  ACNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKKEAIEWAWELLTKVYGL 134

Query: 101 PANRLWISIY--------EEDDEAFAIWHDEVGVPVDRIKRMGEEDNFWTSGITGPCGPC 160
           P +R++ + +        + D+EA  IW     +P  R+   G +DNFW  G TGPCGPC
Sbjct: 135 PTDRIYATYFGGDEKAGLQPDNEARDIWLKV--LPSGRVLPFGCKDNFWEMGDTGPCGPC 194

Query: 161 SEIYYDFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERM 220
           +EI+YD    +  + +   DD   +E +NLVF+Q+N++ DGSL+PL  K++DTG+G ER+
Sbjct: 195 TEIHYDRIGNRDAASLVNNDDPTCLEIWNLVFIQFNRESDGSLKPLPAKHVDTGMGFERL 254

Query: 221 ARILQKVPNNYETDLIYPIIQT------ASELANVIYDTADNQSKTNLKIIGDHMRAVVY 280
             +LQ   +NY+TD+  PI         A   +  +     ++     +++ DH+R + +
Sbjct: 255 TSVLQNKMSNYDTDVFMPIFDDIQKATGARPYSGKVGPEDVDRVDMAYRVVADHIRTLSF 314

Query: 281 LISDGVVPSNIGRGYVVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPLIAEKVIALSSHI 340
            I+DG  P N GR YV+RR++RR VR G+ + +K +     +G F  L++  VI +   +
Sbjct: 315 AIADGSRPGNEGREYVLRRILRRAVRYGKEI-LKAE-----EGFFNGLVS-SVIRVMGDV 374

Query: 341 DEDVKSREGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRVPCLVGKDAFL 400
             ++K  E +I + +K EE  F +TL +G         +    A +  +   L G DAF+
Sbjct: 375 FTELKEHEKKITDIIKEEEASFCKTLAKG--------IEKFRKAGQAVQGNTLSGDDAFI 434

Query: 401 LYDTYGFPVEITTEVAQERGVVVDMKGFDTEMDNQRRQSQAAHN-----AVKLEVGEGAD 460
           L+DTYGFP+++T  +A+ERG++VD+ GF+  M+  R +S++A N     A+ ++    + 
Sbjct: 435 LWDTYGFPLDLTQLMAEERGLLVDVDGFNKAMEEARERSRSAQNKQAGGAIVMDADATST 494

Query: 461 I--MGNISHTEFLGYDLLSAKAIVESLIVNGTSVLQVSEGND-VEILLNRTPFYAESGGQ 520
           +   G  +  +   Y            I  G++ L+ S  +D V ++L  T FYAE GGQ
Sbjct: 495 LHKAGVSATDDSFKYIWFQDHESELKAIYTGSTFLESSAASDNVGLVLGSTSFYAEQGGQ 554

Query: 521 IGDHGFIYISEGENRQNVVVEVKDVQKSAGSIFIHKGTIKQ--GVLEVGREVEAEVDTKL 580
           I D G I  S G         V +VQ   G + +H G + +  G + VG +V  +VD + 
Sbjct: 555 IFDTGLIEGSFG------TFNVCNVQIFGGFV-LHIGYLSKETGEVSVGDKVICKVDYER 614

Query: 581 RQGAKVHHTATHLLQSALKRVIGQETSQAGSLVAFDRLRFDFSYHRPLADTEILKIEELI 640
           R+    +HT TH+L  ALK V+G    Q GS+V  ++LRFDFS+ +P+   ++ +IE ++
Sbjct: 615 RKLIAPNHTCTHMLNYALKEVLGDHIDQKGSIVLPEKLRFDFSHGKPVDPEDLRRIESIV 674

Query: 641 NGWIGDAVLLQTKVMPLVDAKR-AGAVAMFGEKYGEEVRVVEVPG--------------- 700
           N  I D + + +K   L +AKR  G  A+FGE Y + VRVV +                 
Sbjct: 675 NKQIKDELDVFSKEAVLSEAKRIKGLRAVFGEVYPDPVRVVSIGRKVEDLLADPENNEWS 734

Query: 701 -VSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRIEAVAGDAFIEYINARDYHMKRLCT 760
            +S E CGGTH+ NT E + F ++SE+GIA G+RR+ AV  +   + +NA         +
Sbjct: 735 LLSSEFCGGTHITNTREAKAFALLSEEGIAKGIRRVTAVTTECAFDALNA--------AS 794

Query: 761 MLKVKAEDVTTRVENLAEELRLARNEISNLREKTAVFKASSIANKAFVVGTSK-EIRVLV 820
           +L+ + ED              +R E S L +K +  K  S  + A +    K +IR  +
Sbjct: 795 LLEREVEDA-------------SRAEGSALEKKVSALK--SRVDAAIIPAAKKADIRTKI 854

Query: 821 ECMDDTDADSL-----KAAAEFLIDNLQDPAAIVLGSCPGEGKVSLVAAFTPTVVDLGVQ 841
             +    + SL     KA  +    NL+   ++ L +   E   S    F    +D+G+ 
Sbjct: 855 ASLQVISSFSLTNEVRKAQKKIAEQNLK--KSVKLATEAAESAASDGKTFCIIQLDVGLD 876

BLAST of Sed0019949 vs. TAIR 10
Match: AT1G49930.1 (BEST Arabidopsis thaliana protein match is: Alanyl-tRNA synthetase, class IIc (TAIR:AT5G22800.1); Has 21 Blast hits to 21 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 21; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 52.0 bits (123), Expect = 2.9e-06
Identity = 32/72 (44.44%), Postives = 36/72 (50.00%), Query Frame = 0

Query: 777 MDDTDADSLKAAAEFLIDNLQDPAAIVLGSCPGEGKVSLVAAFTPTVVDLGVQAGKFIGP 836
           MDDTDADSLK+AAE LI  L DP  +VLGS P +                          
Sbjct: 1   MDDTDADSLKSAAEHLISTLDDPVVVVLGSSPEKD------------------------- 45

Query: 837 IAKLCGGGGGGR 849
             KLCGGGGGG+
Sbjct: 61  --KLCGGGGGGK 45

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023533450.10.0e+0094.11alanine--tRNA ligase, chloroplastic/mitochondrial isoform X3 [Cucurbita pepo sub... [more]
XP_023533449.10.0e+0094.11alanine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo sub... [more]
XP_023533448.10.0e+0094.11alanine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo sub... [more]
XP_038901232.10.0e+0094.35alanine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida][more]
XP_022970912.10.0e+0093.64alanine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
B9HQZ60.0e+0078.89Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Populus trichocarpa OX=3694... [more]
B8B4H50.0e+0074.09Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica ... [more]
B9FSH50.0e+0073.97Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonic... [more]
Q9FFC70.0e+0075.62Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=370... [more]
C5Z7K40.0e+0073.48Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Sorghum bicolor OX=4558 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1I0H10.0e+0093.64Probable alanine--tRNA ligase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1I5A90.0e+0093.64Probable alanine--tRNA ligase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1I1V00.0e+0093.64Probable alanine--tRNA ligase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1G7M40.0e+0093.64Probable alanine--tRNA ligase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1G7K60.0e+0093.64Probable alanine--tRNA ligase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G22800.10.0e+0075.62Alanyl-tRNA synthetase, class IIc [more]
AT1G50200.12.1e-11833.69Alanyl-tRNA synthetase [more]
AT1G50200.22.8e-11833.61Alanyl-tRNA synthetase [more]
AT1G49930.12.9e-0644.44BEST Arabidopsis thaliana protein match is: Alanyl-tRNA synthetase, class IIc (T... [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 838..865
NoneNo IPR availableCOILSCoilCoilcoord: 995..999
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 100..333
e-value: 6.4E-99
score: 332.4
NoneNo IPR availableGENE3D3.10.310.40coord: 882..990
e-value: 8.1E-25
score: 89.0
NoneNo IPR availableGENE3D3.30.980.10coord: 676..811
e-value: 1.5E-45
score: 156.9
NoneNo IPR availableGENE3D3.30.54.20coord: 737..790
e-value: 1.5E-45
score: 156.9
NoneNo IPR availableGENE3D2.40.30.130coord: 554..665
e-value: 2.3E-16
score: 61.5
NoneNo IPR availableGENE3D6.10.250.550coord: 813..875
e-value: 3.5E-10
score: 41.6
NoneNo IPR availablePANTHERPTHR11777:SF9ALANINE--TRNA LIGASE, MITOCHONDRIALcoord: 100..989
NoneNo IPR availablePANTHERPTHR11777ALANYL-TRNA SYNTHETASEcoord: 100..989
NoneNo IPR availableCDDcd00673AlaRS_corecoord: 103..332
e-value: 1.59334E-128
score: 386.716
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 101..336
IPR002318Alanine-tRNA ligase, class IIcPRINTSPR00980TRNASYNTHALAcoord: 317..330
score: 61.81
coord: 392..405
score: 66.21
coord: 290..301
score: 58.65
coord: 368..384
score: 42.76
coord: 168..179
score: 62.82
IPR002318Alanine-tRNA ligase, class IIcTIGRFAMTIGR00344TIGR00344coord: 105..973
e-value: 1.1E-289
score: 961.4
IPR012947Threonyl/alanyl tRNA synthetase, SADSMARTSM00863tRNA_SAD_4coord: 766..809
e-value: 9.5E-20
score: 81.6
IPR012947Threonyl/alanyl tRNA synthetase, SADPFAMPF07973tRNA_SADcoord: 766..809
e-value: 5.6E-17
score: 61.6
IPR003156DHHA1 domainPFAMPF02272DHHA1coord: 845..988
e-value: 2.3E-19
score: 70.2
IPR018164Alanyl-tRNA synthetase, class IIc, N-terminalPFAMPF01411tRNA-synt_2ccoord: 105..668
e-value: 1.7E-188
score: 627.6
IPR023033Alanine-tRNA ligase, eukaryota/bacteriaHAMAPMF_00036_BAla_tRNA_synth_Bcoord: 100..994
score: 156.863831
IPR018165Alanyl-tRNA synthetase, class IIc, core domainPROSITEPS50860AA_TRNA_LIGASE_II_ALAcoord: 100..822
score: 117.545898
IPR027522Probable alanine--tRNA ligase, plantsHAMAPMF_03134Ala_tRNA_synth_plantCcoord: 92..996
score: 31.824972
IPR009000Translation protein, beta-barrel domain superfamilySUPERFAMILY50447Translation proteinscoord: 561..665
IPR018163Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamilySUPERFAMILY55186ThrRS/AlaRS common domaincoord: 665..826
IPR018162Alanine-tRNA ligase, class IIc, anti-codon-binding domain superfamilySUPERFAMILY101353Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)coord: 337..545

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0019949.1Sed0019949.1mRNA
Sed0019949.2Sed0019949.2mRNA
Sed0019949.3Sed0019949.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009742 brassinosteroid mediated signaling pathway
biological_process GO:0006419 alanyl-tRNA aminoacylation
biological_process GO:0006468 protein phosphorylation
biological_process GO:0070143 mitochondrial alanyl-tRNA aminoacylation
biological_process GO:0043039 tRNA aminoacylation
cellular_component GO:0009507 chloroplast
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0000049 tRNA binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004813 alanine-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding