Sed0019853 (gene) Chayote v1

BLAST of Sed0019853 vs. NCBI nr
Match: XP_023533619.1 (magnesium transporter MRS2-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.8 bits (1971), Expect = 8.0e-217
Identity = 403/452 (89.16%), Postives = 418/452 (92.48%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSS--------GAPVG 62
           GKGPFSFRRS+SRRRPKK  QS+PL SPQ    I ASSPP N+NGSS        G  VG
Sbjct: 2   GKGPFSFRRSNSRRRPKKAAQSTPLLSPQTPLPIDASSPPINNNGSSLVGGGGGGGGAVG 61

Query: 63  KVKKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREK 122
           KVKKKAGGA+LWMRLDRWGQSE+LEWDK+AIIRRAAIPARDLRILGPVFSHSSNILAREK
Sbjct: 62  KVKKKAGGAKLWMRLDRWGQSELLEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREK 121

Query: 123 AMVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDV 182
           AMVVNLEFIKAIVTAEEVLL DPLRQEVIPFV+QLRKLLA  SSSQ HGDGTVDERENDV
Sbjct: 122 AMVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSSQGHGDGTVDERENDV 181

Query: 183 NVSRGGKWLPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELAR 242
           NVS GGKWLPVSEA EG QYELPFEFQVLE+ALEVVC+YLDSSV DLERDAYPVLDELAR
Sbjct: 182 NVSSGGKWLPVSEAAEGVQYELPFEFQVLENALEVVCMYLDSSVEDLERDAYPVLDELAR 241

Query: 243 NVSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALL 302
           NVSTKNLERVRSLKSNLTRILARVQKVRDE+EHLLDDNEDMAQLYLTRKWMQNQQA+ALL
Sbjct: 242 NVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQADALL 301

Query: 303 GGLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILS 362
           GGLASNSTTAIVPHLRRLSS RSGS+VTSNLLD NDVEDLEMLLEAYFMQLDGTRNRILS
Sbjct: 302 GGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTRNRILS 361

Query: 363 VREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYDTEG 422
           VREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAG FGMNIHCTLYD EG
Sbjct: 362 VREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTLYDQEG 421

Query: 423 IFEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           IFEY VGGT+ GCLLIFVLI GYA+WKKLLGS
Sbjct: 422 IFEYFVGGTSVGCLLIFVLIFGYAKWKKLLGS 453

BLAST of Sed0019853 vs. NCBI nr
Match: XP_022136744.1 (magnesium transporter MRS2-4 [Momordica charantia])

HSP 1 Score: 756.5 bits (1952), Expect = 1.3e-214
Identity = 403/451 (89.36%), Postives = 422/451 (93.57%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSS-----GAPVGKVK 62
           GKGPFSFRRSSSRRRPKK  Q SP  SPQP P IG SSPP N+N S+     G  VGKVK
Sbjct: 2   GKGPFSFRRSSSRRRPKKAAQLSP--SPQPPP-IGVSSPPINNNSSAMVSGGGGAVGKVK 61

Query: 63  KKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAMV 122
           KKAGGARLWMRLDRWGQSE++EWDK+AIIRRAAIPARDLRILGPVFSHSSNILAREKAMV
Sbjct: 62  KKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKAMV 121

Query: 123 VNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQV--HGDGTVDERENDVN 182
           VNLEFIKA+VTAEEVLLLDPLRQEVIPFV+QLRKLLAKNSSSQ   HGDGTVDERENDVN
Sbjct: 122 VNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDERENDVN 181

Query: 183 VSRGGKWLPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARN 242
           VSRGGKWLPVSEAVEGEQYELPFEFQVLE+ALEVVC+YLDSSVADLERDAYPVLDELARN
Sbjct: 182 VSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELARN 241

Query: 243 VSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALLG 302
           VSTKNLE VRSLKSNLTRILARVQKVRDE+EHLLDDNEDMAQLYLTRKWMQNQQ +ALLG
Sbjct: 242 VSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDALLG 301

Query: 303 GLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSV 362
           G+ASNSTTAIVP+LRRLSS +SGSVVTSN LD NDVEDLEMLLEAYFMQLDGTRNRILSV
Sbjct: 302 GMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRILSV 361

Query: 363 REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYDTEGI 422
           REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAG FGMNI CTLY+ +GI
Sbjct: 362 REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNKDGI 421

Query: 423 FEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           FEY VGGT+AGCLLIF+LI+GYARWKKLLGS
Sbjct: 422 FEYFVGGTSAGCLLIFLLILGYARWKKLLGS 449

BLAST of Sed0019853 vs. NCBI nr
Match: XP_022942922.1 (magnesium transporter MRS2-4-like [Cucurbita moschata])

HSP 1 Score: 755.4 bits (1949), Expect = 2.8e-214
Identity = 401/455 (88.13%), Postives = 416/455 (91.43%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSS--------GAPVG 62
           GKGPFSFRRS+SRRRPKK  QS+PL SPQ    I  +SPP N+NGSS        G  VG
Sbjct: 2   GKGPFSFRRSNSRRRPKKAAQSTPLLSPQTPLPIDVNSPPINNNGSSLVGGGGGGGGAVG 61

Query: 63  KVKKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREK 122
           KVKKKAGGA+LWMRLDRWGQSE+LEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREK
Sbjct: 62  KVKKKAGGAKLWMRLDRWGQSELLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREK 121

Query: 123 AMVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDV 182
           AMVVNLEFIKAIVTAEEVLL DPLRQEVIPFV+QLRKLLA  SSSQ HGDGTVDERENDV
Sbjct: 122 AMVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSSQGHGDGTVDERENDV 181

Query: 183 NVSRGGKWLPVS---EAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDE 242
           NVS GGKWLPVS   EA EG  YELPFEFQVLE+ALEVVC+YLDSSV DLERDAYPVLDE
Sbjct: 182 NVSSGGKWLPVSEAAEAAEGVPYELPFEFQVLENALEVVCMYLDSSVEDLERDAYPVLDE 241

Query: 243 LARNVSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAE 302
           LARNVSTKNLERVRSLKSNLTRILARVQKVRDE+EHLLDDNEDMAQLYLTRKWMQNQQA+
Sbjct: 242 LARNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQAD 301

Query: 303 ALLGGLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNR 362
           ALLGGLASNSTTAIVPHLRRLSS RSGS+VTSNLLD NDVEDLEMLLEAYFMQLDGTRNR
Sbjct: 302 ALLGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTRNR 361

Query: 363 ILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYD 422
           ILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAG FGMNIHCTLYD
Sbjct: 362 ILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTLYD 421

Query: 423 TEGIFEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
            EGIFEY VGGT+ GCLLIFVLI GYA+WKKLLGS
Sbjct: 422 REGIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLGS 456

BLAST of Sed0019853 vs. NCBI nr
Match: XP_022980784.1 (magnesium transporter MRS2-4-like [Cucurbita maxima])

HSP 1 Score: 751.9 bits (1940), Expect = 3.1e-213
Identity = 399/457 (87.31%), Postives = 416/457 (91.03%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSS----------GAP 62
           GKGPFSFRRS+SRRRPKK  QS+PL SPQ    I  SSPP N+NGSS          G  
Sbjct: 2   GKGPFSFRRSNSRRRPKKAAQSTPLLSPQTPLPIDVSSPPINNNGSSLVGGGGGGGGGGA 61

Query: 63  VGKVKKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAR 122
           VGKVKKKAGGA+LWMRLDRWGQSE+LEWDK+AIIRRAAIPARDLRILGPVFSHSSNILAR
Sbjct: 62  VGKVKKKAGGAKLWMRLDRWGQSELLEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAR 121

Query: 123 EKAMVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDEREN 182
           EKAMVVNLEFIKAIVTAEEVLL DPLRQEVIPFV+QLRKLLA  SSSQ HGDG VDEREN
Sbjct: 122 EKAMVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSSQGHGDGAVDEREN 181

Query: 183 DVNVSRGGKWLPVS---EAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVL 242
           DVNVS GGKWLPVS   EA EG QYELPFEFQVLE+ALEVVC+YLDSSV DLE+DAYPVL
Sbjct: 182 DVNVSSGGKWLPVSEAAEAAEGVQYELPFEFQVLENALEVVCMYLDSSVEDLEKDAYPVL 241

Query: 243 DELARNVSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQ 302
           DELARNVSTKNLERVRSLKSNLTRILARVQKVRDE+EHLLDDNEDMAQLYLTRKWMQNQQ
Sbjct: 242 DELARNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ 301

Query: 303 AEALLGGLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTR 362
           ++ALLGGLASNSTTAIVPHLRRLSS RSGS+VTSNLLD NDVEDLEMLLEAYFMQLDGTR
Sbjct: 302 SDALLGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTR 361

Query: 363 NRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTL 422
           NRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAG FGMNIHCTL
Sbjct: 362 NRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTL 421

Query: 423 YDTEGIFEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           YD EGIFEY VGGT+ GCLLIFVLI GYA+WKKLLGS
Sbjct: 422 YDREGIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLGS 458

BLAST of Sed0019853 vs. NCBI nr
Match: KAG7031030.1 (Magnesium transporter MRS2-4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 751.9 bits (1940), Expect = 3.1e-213
Identity = 400/457 (87.53%), Postives = 415/457 (90.81%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGS----------SGAP 62
           GKGPFSFRRS+SRRRPKK  QS+PL SPQ    I A+SPP N+NGS           G  
Sbjct: 2   GKGPFSFRRSNSRRRPKKAAQSTPLLSPQTPLPIDATSPPINNNGSGLVGGGGGGGGGGT 61

Query: 63  VGKVKKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAR 122
           VGKVKKKAGGA+LWMRLDRWGQSE+LEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAR
Sbjct: 62  VGKVKKKAGGAKLWMRLDRWGQSELLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAR 121

Query: 123 EKAMVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDEREN 182
           EKAMVVNLEFIKAIVTAEEVLL DPLRQEVIPFV+QLRKLLA  SSSQ HGDGTVDEREN
Sbjct: 122 EKAMVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSSQGHGDGTVDEREN 181

Query: 183 DVNVSRGGKWLPVS---EAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVL 242
            VNVS GGKWLPVS   EA EG QYELPFEFQVLE+ALEVVC+YLDSSV DLERDAYPVL
Sbjct: 182 VVNVSSGGKWLPVSEAGEAAEGVQYELPFEFQVLENALEVVCMYLDSSVEDLERDAYPVL 241

Query: 243 DELARNVSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQ 302
           DELARNVSTKNLERVRSLKSNLTRILARVQKVRDE+EHLLDDNEDMAQLYLTRKWMQNQQ
Sbjct: 242 DELARNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ 301

Query: 303 AEALLGGLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTR 362
           A+A LGGLASNSTTAIVPHLRRLSS RSGS+VTSNLLD NDVEDLEMLLEAYFMQLDGTR
Sbjct: 302 ADAFLGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTR 361

Query: 363 NRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTL 422
           NRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAG FGMNIHCTL
Sbjct: 362 NRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTL 421

Query: 423 YDTEGIFEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           YD EGIFEY VGGT+ GCLLIFVLI GYA+WKKLLGS
Sbjct: 422 YDREGIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLGS 458

BLAST of Sed0019853 vs. ExPASy Swiss-Prot
Match: Q93ZD7 (Magnesium transporter MRS2-4 OS=Arabidopsis thaliana OX=3702 GN=MRS2-4 PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 3.8e-145
Identity = 295/448 (65.85%), Postives = 338/448 (75.45%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSSGAPVGKVKKKAGG 62
           GKGP SFRR SS R  KK       S+   TP       P ++ GS+    GK KKK GG
Sbjct: 2   GKGPLSFRRLSSIRHRKKGSAVKDDSAQTSTPSSPPPPLPIHAGGSAVGATGKAKKKTGG 61

Query: 63  ARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAMVVNLEF 122
           ARLWMR DR G  EV+E DK+ II+RA++PARDLRILGPVFSHSSNILAREKA+VVNLE 
Sbjct: 62  ARLWMRFDRTGAMEVVECDKSTIIKRASVPARDLRILGPVFSHSSNILAREKAIVVNLEV 121

Query: 123 IKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDVNVSRGGKW 182
           IKAIVTAEEVLLLDPLR EV+PFVE+L++   + + +           EN +  S   + 
Sbjct: 122 IKAIVTAEEVLLLDPLRPEVLPFVERLKQQFPQRNGN-----------ENALQASANVQS 181

Query: 183 LPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARNVSTKNLE 242
               EA EG Q ELPFEFQVLE ALEVVC ++D SVA LE +A+PVLDEL +NVST+NLE
Sbjct: 182 PLDPEAAEGLQSELPFEFQVLEIALEVVCSFVDKSVAALETEAWPVLDELTKNVSTENLE 241

Query: 243 RVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALLGGLASNST 302
            VRSLKSNLTR+LARVQKVRDELEHLLDDNEDMA LYLTRKW+QNQQ EA+L G ASNS 
Sbjct: 242 YVRSLKSNLTRLLARVQKVRDELEHLLDDNEDMADLYLTRKWIQNQQTEAILAGTASNS- 301

Query: 303 TAIVPH----LRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSVREY 362
            A+  H    L RL+S RS S+VTSN  + +DVEDLEMLLEAYFMQLDG RN+IL+VREY
Sbjct: 302 IALPAHNTSNLHRLTSNRSASMVTSN-TEEDDVEDLEMLLEAYFMQLDGMRNKILTVREY 361

Query: 363 IDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYDTEGIFEY 422
           IDDTEDYVNIQLDNQRNELIQ QLTLTIASFAIA ETL+A  FGMNI C LY   G+F Y
Sbjct: 362 IDDTEDYVNIQLDNQRNELIQLQLTLTIASFAIAAETLLASLFGMNIPCPLYSIHGVFGY 421

Query: 423 LVGGTTAGCLLIFVLIIGYARWKKLLGS 447
            V   TA C+++F++ +GYARWKKLLGS
Sbjct: 422 FVWSVTALCIVLFMVTLGYARWKKLLGS 436

BLAST of Sed0019853 vs. ExPASy Swiss-Prot
Match: A3BV82 (Putative magnesium transporter MRS2-G OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-G PE=1 SV=2)

HSP 1 Score: 467.6 bits (1202), Expect = 1.6e-130
Identity = 276/442 (62.44%), Postives = 318/442 (71.95%), Query Frame = 0

Query: 8   SFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSSGAPVGKVKKKAGGARLWM 67
           S RR  S  RP+     SP S   P P     +PP+     S A  GKV KK  GARLWM
Sbjct: 28  SARRLPSLTRPRASSSPSPASPSPPPPSASHPAPPSPPLAVSPAGAGKVGKKKAGARLWM 87

Query: 68  RLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAMVVNLEFIKAIV 127
           RLDRWG SE L  DK +IIRRA +P RDLRILGPVFS SS+ILAREKAMV+NLEFI+AIV
Sbjct: 88  RLDRWGVSETLHLDKGSIIRRAGLPPRDLRILGPVFSDSSSILAREKAMVINLEFIRAIV 147

Query: 128 TAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDVNVSRGGKWLPVSE 187
           TA+E+LLLDPL  +VIPFVEQL   L   +    +G    D+     + S G +   ++E
Sbjct: 148 TADEILLLDPLTIDVIPFVEQLTHHLPLKNLVCGNGQPGGDDHGEKHDDSHGDQVPRLNE 207

Query: 188 AVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARNVSTKNLERVRSL 247
           A  G ++ELPFEFQVLE ALE VC   D +V+ LER A PVL+EL +NVST+NL+RVR+L
Sbjct: 208 AT-GAEHELPFEFQVLELALETVCSSFDVNVSGLERRATPVLEELTKNVSTRNLDRVRTL 267

Query: 248 KSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALLGGLASNSTTAIVP 307
           KS+LTR+LA VQKVRDE+EHLLDDNEDMA LYLTRK +QNQQ EAL+   ASNS      
Sbjct: 268 KSDLTRLLAHVQKVRDEIEHLLDDNEDMAHLYLTRKQLQNQQVEALISSAASNSIVPGGT 327

Query: 308 HLRRLSSV--RSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSVREYIDDTEDY 367
            L RL++   RS S+ TS  LD NDVEDLEMLLEAYFMQLDG RNRILSVREYIDDTEDY
Sbjct: 328 SLSRLNNSFRRSVSIATSMHLD-NDVEDLEMLLEAYFMQLDGIRNRILSVREYIDDTEDY 387

Query: 368 VNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLY--DTEGIFEYLVGGT 427
           VNIQLDNQRNELIQ QLTLTIASF IAV T IAG F MNI   LY  D    F   VGGT
Sbjct: 388 VNIQLDNQRNELIQLQLTLTIASFGIAVNTFIAGAFAMNIQSKLYSIDDGSFFWPFVGGT 447

Query: 428 TAGCLLIFVLIIGYARWKKLLG 446
           ++GC +I ++++ YARWKKLLG
Sbjct: 448 SSGCFMICIVLLWYARWKKLLG 467

BLAST of Sed0019853 vs. ExPASy Swiss-Prot
Match: A2Z9W7 (Putative magnesium transporter MRS2-G OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-G PE=3 SV=2)

HSP 1 Score: 466.8 bits (1200), Expect = 2.7e-130
Identity = 276/442 (62.44%), Postives = 318/442 (71.95%), Query Frame = 0

Query: 8   SFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSSGAPVGKVKKKAGGARLWM 67
           S RR  S  RP+     SP S   P P     +PP+     S A  GKV KK  GARLWM
Sbjct: 28  SARRLPSLTRPRASSSPSPASPSPPPPSASHPAPPSPPLAVSPAGAGKVGKKKAGARLWM 87

Query: 68  RLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAMVVNLEFIKAIV 127
           RLDRWG SE L  DK +IIRRA +P RDLRILGPVFS SS+ILAREKAMV+NLEFI+AIV
Sbjct: 88  RLDRWGVSETLHLDKGSIIRRAGLPPRDLRILGPVFSDSSSILAREKAMVINLEFIRAIV 147

Query: 128 TAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDVNVSRGGKWLPVSE 187
           TA+E+LLLDPL  +VIPFVEQL   L   +    +G    D+     + S G +   ++E
Sbjct: 148 TADEILLLDPLTIDVIPFVEQLTHHLPLKNLVCGNGQPGGDDHGEKHDDSPGDQVPRLNE 207

Query: 188 AVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARNVSTKNLERVRSL 247
           A  G ++ELPFEFQVLE ALE VC   D +V+ LER A PVL+EL +NVST+NL+RVR+L
Sbjct: 208 AT-GAEHELPFEFQVLELALETVCSSFDVNVSGLERRATPVLEELTKNVSTRNLDRVRTL 267

Query: 248 KSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALLGGLASNSTTAIVP 307
           KS+LTR+LA VQKVRDE+EHLLDDNEDMA LYLTRK +QNQQ EAL+   ASNS      
Sbjct: 268 KSDLTRLLAHVQKVRDEIEHLLDDNEDMAHLYLTRKQLQNQQVEALISSAASNSIVPGGT 327

Query: 308 HLRRLSSV--RSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSVREYIDDTEDY 367
            L RL++   RS S+ TS  LD NDVEDLEMLLEAYFMQLDG RNRILSVREYIDDTEDY
Sbjct: 328 SLSRLNNSFRRSVSIATSMHLD-NDVEDLEMLLEAYFMQLDGIRNRILSVREYIDDTEDY 387

Query: 368 VNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLY--DTEGIFEYLVGGT 427
           VNIQLDNQRNELIQ QLTLTIASF IAV T IAG F MNI   LY  D    F   VGGT
Sbjct: 388 VNIQLDNQRNELIQLQLTLTIASFGIAVNTFIAGAFAMNIQSKLYSIDDGSFFWPFVGGT 447

Query: 428 TAGCLLIFVLIIGYARWKKLLG 446
           ++GC +I ++++ YARWKKLLG
Sbjct: 448 SSGCFMICIVLLWYARWKKLLG 467

BLAST of Sed0019853 vs. ExPASy Swiss-Prot
Match: Q1PE39 (Magnesium transporter MRS2-6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MRS2-6 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.2e-90
Identity = 206/437 (47.14%), Postives = 277/437 (63.39%), Query Frame = 0

Query: 8   SFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSSGAPVGKVKKKAGGARLWM 67
           S RRS+S R  KK         P       + SPPT    +     GK KK+ GG  LW 
Sbjct: 3   SLRRSTSNRSRKKGTAVKMNRMP------SSLSPPTPPCSAIVGGTGKSKKRRGGVCLWT 62

Query: 68  RLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAMVVNLEFIKAIV 127
           R DR G  EV   DK+ II R+++ A+DLR     FSHSS ILAREKA+V+NLE IKA++
Sbjct: 63  RFDRTGFMEVAGCDKSTIIERSSVSAKDLR---TAFSHSSKILAREKAIVLNLEVIKAVI 122

Query: 128 TAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDVNVSRGGKWLPVSE 187
           T+E+V+LLD LR EV+   ++L+           H     D  EN +  S  G       
Sbjct: 123 TSEQVMLLDSLRPEVLTLTDRLK-----------HHFPRKDGPENILQASSHGH---QEG 182

Query: 188 AVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARNVSTKNLERVRSL 247
             EG + +LPFEF+VLE A EV C ++DS+V DLE  A+ +LDEL + VS +NL+ +RSL
Sbjct: 183 GEEGLKSKLPFEFRVLEIAFEVFCSFVDSNVVDLETQAWSILDELTKKVSNENLKDLRSL 242

Query: 248 KSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALLGGLASNSTTAIVP 307
           K++LT +LARVQKVRDE+EH LDD EDM  LYLTRKW+QNQQ EA     ASNS  +  P
Sbjct: 243 KTSLTHLLARVQKVRDEIEHFLDDKEDMEDLYLTRKWIQNQQTEA-----ASNSIVS-QP 302

Query: 308 HLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSVREYIDDTEDYVN 367
           +L+R +S R   + TS + + +D++D+EMLLEAYFMQL+G RN+IL ++E+ID TE YV 
Sbjct: 303 NLQRHTSNR---ISTSMVTEEDDIDDMEMLLEAYFMQLEGMRNKILLMKEHIDSTEAYVK 362

Query: 368 IQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYDTEGIFEYLVGGTTAGC 427
           I  +++RN LI   + + I ++AI   T++   FGMNI   LY T  IF Y+V    A C
Sbjct: 363 ILQNSRRNGLIHLMMLVNIGNYAITAGTVVVNLFGMNIPIGLYSTPDIFGYVVWAVVALC 407

Query: 428 LLIFVLIIGYARWKKLL 445
           +++F++ +GYA+WKKLL
Sbjct: 423 IVLFIVTVGYAKWKKLL 407

BLAST of Sed0019853 vs. ExPASy Swiss-Prot
Match: A2XCA0 (Putative magnesium transporter MRS2-H OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-H PE=3 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 6.0e-90
Identity = 216/445 (48.54%), Postives = 281/445 (63.15%), Query Frame = 0

Query: 8   SFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSSGAPVGKVKKKAGGARLWM 67
           SF  S   RR + V +    S P+P       +PP    G      G  K+KA   RLWM
Sbjct: 18  SFSSSPESRRCRSVHRVP--SRPRP-----PLAPPARVMGK-----GNSKRKAANTRLWM 77

Query: 68  RLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAMVVNLEFIKAIV 127
           RLDR G  E++  DK+ + RR+ +PARDLR+LGP+ S S +ILAREKAMV+NLEF++AIV
Sbjct: 78  RLDRRGGCEMILCDKSFVARRSGLPARDLRVLGPLLSRSPSILAREKAMVINLEFVRAIV 137

Query: 128 TAEEVLLLDPLRQEVIPFVEQLRK------LLAKNSSSQVHGDGTVDERENDVNVSRGGK 187
           TA+EVL+L+PL QEV+PFVE+LRK      L   + S+ +H +    E   DV+      
Sbjct: 138 TADEVLVLEPLAQEVLPFVEKLRKHFPLKSLDVDDVSTHMHTENQDGELAQDVSCYE--- 197

Query: 188 WLPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARNVSTKNL 247
                  VEG  +ELPFEFQVL+ ALE VCL  +S+++DL R A  VLD+L ++VST+NL
Sbjct: 198 -------VEGANHELPFEFQVLDFALEAVCLSYNSTISDLNRSAIAVLDDLMKSVSTRNL 257

Query: 248 ERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALLGGLASNS 307
           ERVRSLKS+LTR+LA VQKVRDE+EH+LDDNE MA L   RK    +  + LL  +    
Sbjct: 258 ERVRSLKSSLTRLLASVQKVRDEVEHILDDNEAMAHLCTARK---TKGQKDLLNTILFPE 317

Query: 308 TTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSVREYIDD 367
           T     H    SS+ + + + + +   +D   L+MLLEAYF QLDG RNRI  VR+YI D
Sbjct: 318 TRLCRTH----SSIENSTGIRTCVPSDSDAHILDMLLEAYFKQLDGIRNRIFLVRQYIVD 377

Query: 368 TEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNI-HCTLYDTEGI-FEYL 427
           TEDY++IQLDN+RNEL+  QLTL IASF IA+ T IA  F MNI H   +   G+ F   
Sbjct: 378 TEDYISIQLDNKRNELLGLQLTLIIASFGIAINTFIAAAFAMNIPHRGYHFVIGVPFGQF 433

Query: 428 VGGTTAGCLLIFVLIIGYARWKKLL 445
           VG T+  C+ I +L+  YA   +LL
Sbjct: 438 VGATSFLCMSIVILLFTYAWRNRLL 433

BLAST of Sed0019853 vs. ExPASy TrEMBL
Match: A0A6J1C580 (Magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111008374 PE=3 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 6.2e-215
Identity = 403/451 (89.36%), Postives = 422/451 (93.57%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSS-----GAPVGKVK 62
           GKGPFSFRRSSSRRRPKK  Q SP  SPQP P IG SSPP N+N S+     G  VGKVK
Sbjct: 2   GKGPFSFRRSSSRRRPKKAAQLSP--SPQPPP-IGVSSPPINNNSSAMVSGGGGAVGKVK 61

Query: 63  KKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAMV 122
           KKAGGARLWMRLDRWGQSE++EWDK+AIIRRAAIPARDLRILGPVFSHSSNILAREKAMV
Sbjct: 62  KKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKAMV 121

Query: 123 VNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQV--HGDGTVDERENDVN 182
           VNLEFIKA+VTAEEVLLLDPLRQEVIPFV+QLRKLLAKNSSSQ   HGDGTVDERENDVN
Sbjct: 122 VNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDERENDVN 181

Query: 183 VSRGGKWLPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARN 242
           VSRGGKWLPVSEAVEGEQYELPFEFQVLE+ALEVVC+YLDSSVADLERDAYPVLDELARN
Sbjct: 182 VSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELARN 241

Query: 243 VSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALLG 302
           VSTKNLE VRSLKSNLTRILARVQKVRDE+EHLLDDNEDMAQLYLTRKWMQNQQ +ALLG
Sbjct: 242 VSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDALLG 301

Query: 303 GLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSV 362
           G+ASNSTTAIVP+LRRLSS +SGSVVTSN LD NDVEDLEMLLEAYFMQLDGTRNRILSV
Sbjct: 302 GMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRILSV 361

Query: 363 REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYDTEGI 422
           REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAG FGMNI CTLY+ +GI
Sbjct: 362 REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNKDGI 421

Query: 423 FEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           FEY VGGT+AGCLLIF+LI+GYARWKKLLGS
Sbjct: 422 FEYFVGGTSAGCLLIFLLILGYARWKKLLGS 449

BLAST of Sed0019853 vs. ExPASy TrEMBL
Match: A0A6J1FRL4 (Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111447807 PE=3 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.4e-214
Identity = 401/455 (88.13%), Postives = 416/455 (91.43%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSS--------GAPVG 62
           GKGPFSFRRS+SRRRPKK  QS+PL SPQ    I  +SPP N+NGSS        G  VG
Sbjct: 2   GKGPFSFRRSNSRRRPKKAAQSTPLLSPQTPLPIDVNSPPINNNGSSLVGGGGGGGGAVG 61

Query: 63  KVKKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREK 122
           KVKKKAGGA+LWMRLDRWGQSE+LEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREK
Sbjct: 62  KVKKKAGGAKLWMRLDRWGQSELLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREK 121

Query: 123 AMVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDV 182
           AMVVNLEFIKAIVTAEEVLL DPLRQEVIPFV+QLRKLLA  SSSQ HGDGTVDERENDV
Sbjct: 122 AMVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSSQGHGDGTVDERENDV 181

Query: 183 NVSRGGKWLPVS---EAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDE 242
           NVS GGKWLPVS   EA EG  YELPFEFQVLE+ALEVVC+YLDSSV DLERDAYPVLDE
Sbjct: 182 NVSSGGKWLPVSEAAEAAEGVPYELPFEFQVLENALEVVCMYLDSSVEDLERDAYPVLDE 241

Query: 243 LARNVSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAE 302
           LARNVSTKNLERVRSLKSNLTRILARVQKVRDE+EHLLDDNEDMAQLYLTRKWMQNQQA+
Sbjct: 242 LARNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQAD 301

Query: 303 ALLGGLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNR 362
           ALLGGLASNSTTAIVPHLRRLSS RSGS+VTSNLLD NDVEDLEMLLEAYFMQLDGTRNR
Sbjct: 302 ALLGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTRNR 361

Query: 363 ILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYD 422
           ILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAG FGMNIHCTLYD
Sbjct: 362 ILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTLYD 421

Query: 423 TEGIFEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
            EGIFEY VGGT+ GCLLIFVLI GYA+WKKLLGS
Sbjct: 422 REGIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLGS 456

BLAST of Sed0019853 vs. ExPASy TrEMBL
Match: A0A6J1J073 (Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111480067 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.5e-213
Identity = 399/457 (87.31%), Postives = 416/457 (91.03%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSS----------GAP 62
           GKGPFSFRRS+SRRRPKK  QS+PL SPQ    I  SSPP N+NGSS          G  
Sbjct: 2   GKGPFSFRRSNSRRRPKKAAQSTPLLSPQTPLPIDVSSPPINNNGSSLVGGGGGGGGGGA 61

Query: 63  VGKVKKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAR 122
           VGKVKKKAGGA+LWMRLDRWGQSE+LEWDK+AIIRRAAIPARDLRILGPVFSHSSNILAR
Sbjct: 62  VGKVKKKAGGAKLWMRLDRWGQSELLEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAR 121

Query: 123 EKAMVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDEREN 182
           EKAMVVNLEFIKAIVTAEEVLL DPLRQEVIPFV+QLRKLLA  SSSQ HGDG VDEREN
Sbjct: 122 EKAMVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSSQGHGDGAVDEREN 181

Query: 183 DVNVSRGGKWLPVS---EAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVL 242
           DVNVS GGKWLPVS   EA EG QYELPFEFQVLE+ALEVVC+YLDSSV DLE+DAYPVL
Sbjct: 182 DVNVSSGGKWLPVSEAAEAAEGVQYELPFEFQVLENALEVVCMYLDSSVEDLEKDAYPVL 241

Query: 243 DELARNVSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQ 302
           DELARNVSTKNLERVRSLKSNLTRILARVQKVRDE+EHLLDDNEDMAQLYLTRKWMQNQQ
Sbjct: 242 DELARNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ 301

Query: 303 AEALLGGLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTR 362
           ++ALLGGLASNSTTAIVPHLRRLSS RSGS+VTSNLLD NDVEDLEMLLEAYFMQLDGTR
Sbjct: 302 SDALLGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTR 361

Query: 363 NRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTL 422
           NRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAG FGMNIHCTL
Sbjct: 362 NRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTL 421

Query: 423 YDTEGIFEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           YD EGIFEY VGGT+ GCLLIFVLI GYA+WKKLLGS
Sbjct: 422 YDREGIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLGS 458

BLAST of Sed0019853 vs. ExPASy TrEMBL
Match: A0A1S3C2A5 (Magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103496094 PE=3 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.6e-207
Identity = 389/451 (86.25%), Postives = 411/451 (91.13%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGS------SGAPVGKV 62
           GKGPFSFRRSSSRRRPKK VQSSP SSPQ  P++  SSPP N+NG+       G  VGKV
Sbjct: 2   GKGPFSFRRSSSRRRPKKAVQSSPSSSPQLPPQVSTSSPPINNNGNGLVGGGGGGAVGKV 61

Query: 63  KKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAM 122
           KKKAGG+RLWMRLDRWGQSE+LEWDKNAIIRR  IPARD+RILGPVFSHSSNILAREKAM
Sbjct: 62  KKKAGGSRLWMRLDRWGQSELLEWDKNAIIRRVGIPARDMRILGPVFSHSSNILAREKAM 121

Query: 123 VVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDVNV 182
           VVNLEFIKAIVTAEEVLLLDPLRQEVIPFV+QLRKLLAK  SSQ+         END NV
Sbjct: 122 VVNLEFIKAIVTAEEVLLLDPLRQEVIPFVDQLRKLLAKTGSSQL---------ENDGNV 181

Query: 183 SRGGKWLPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARNV 242
           SRGGKWLPVSEA EGEQYELPFEFQVLE+ALEVVCLYLDSSVADLERDAYPVLDELARNV
Sbjct: 182 SRGGKWLPVSEAAEGEQYELPFEFQVLENALEVVCLYLDSSVADLERDAYPVLDELARNV 241

Query: 243 STKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALL-G 302
           STKNLERVRSLKSNLTRILARVQKVRDE+EHLLDDNEDMAQLYLTRKWMQNQQ +AL+ G
Sbjct: 242 STKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQFDALMGG 301

Query: 303 GLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSV 362
           G ASNSTTA+VPHLRRLSS RSGSVVTSNLLD NDVEDLEMLLEAYFMQLDGTRNRILSV
Sbjct: 302 GGASNSTTAVVPHLRRLSSNRSGSVVTSNLLDDNDVEDLEMLLEAYFMQLDGTRNRILSV 361

Query: 363 REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYDTEGI 422
           REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAG FGMNI CTLY+T+G+
Sbjct: 362 REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYETDGV 421

Query: 423 FEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           F Y VGGT+AGCLL+F+LI+GYA+WKKLLGS
Sbjct: 422 FGYFVGGTSAGCLLLFLLILGYAKWKKLLGS 443

BLAST of Sed0019853 vs. ExPASy TrEMBL
Match: A0A5D3CIR6 (Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00310 PE=3 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.6e-207
Identity = 389/451 (86.25%), Postives = 411/451 (91.13%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGS------SGAPVGKV 62
           GKGPFSFRRSSSRRRPKK VQSSP SSPQ  P++  SSPP N+NG+       G  VGKV
Sbjct: 2   GKGPFSFRRSSSRRRPKKAVQSSPSSSPQLPPQVSTSSPPINNNGNGLVGGGGGGAVGKV 61

Query: 63  KKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAM 122
           KKKAGG+RLWMRLDRWGQSE+LEWDKNAIIRR  IPARD+RILGPVFSHSSNILAREKAM
Sbjct: 62  KKKAGGSRLWMRLDRWGQSELLEWDKNAIIRRVGIPARDMRILGPVFSHSSNILAREKAM 121

Query: 123 VVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDVNV 182
           VVNLEFIKAIVTAEEVLLLDPLRQEVIPFV+QLRKLLAK  SSQ+         END NV
Sbjct: 122 VVNLEFIKAIVTAEEVLLLDPLRQEVIPFVDQLRKLLAKTGSSQL---------ENDGNV 181

Query: 183 SRGGKWLPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARNV 242
           SRGGKWLPVSEA EGEQYELPFEFQVLE+ALEVVCLYLDSSVADLERDAYPVLDELARNV
Sbjct: 182 SRGGKWLPVSEAAEGEQYELPFEFQVLENALEVVCLYLDSSVADLERDAYPVLDELARNV 241

Query: 243 STKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALL-G 302
           STKNLERVRSLKSNLTRILARVQKVRDE+EHLLDDNEDMAQLYLTRKWMQNQQ +AL+ G
Sbjct: 242 STKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQFDALMGG 301

Query: 303 GLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSV 362
           G ASNSTTA+VPHLRRLSS RSGSVVTSNLLD NDVEDLEMLLEAYFMQLDGTRNRILSV
Sbjct: 302 GGASNSTTAVVPHLRRLSSNRSGSVVTSNLLDDNDVEDLEMLLEAYFMQLDGTRNRILSV 361

Query: 363 REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYDTEGI 422
           REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAG FGMNI CTLY+T+G+
Sbjct: 362 REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYETDGV 421

Query: 423 FEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           F Y VGGT+AGCLL+F+LI+GYA+WKKLLGS
Sbjct: 422 FGYFVGGTSAGCLLLFLLILGYAKWKKLLGS 443

BLAST of Sed0019853 vs. TAIR 10
Match: AT3G58970.1 (magnesium transporter 6 )

HSP 1 Score: 516.2 bits (1328), Expect = 2.7e-146
Identity = 295/448 (65.85%), Postives = 338/448 (75.45%), Query Frame = 0

Query: 3   GKGPFSFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSSGAPVGKVKKKAGG 62
           GKGP SFRR SS R  KK       S+   TP       P ++ GS+    GK KKK GG
Sbjct: 2   GKGPLSFRRLSSIRHRKKGSAVKDDSAQTSTPSSPPPPLPIHAGGSAVGATGKAKKKTGG 61

Query: 63  ARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAMVVNLEF 122
           ARLWMR DR G  EV+E DK+ II+RA++PARDLRILGPVFSHSSNILAREKA+VVNLE 
Sbjct: 62  ARLWMRFDRTGAMEVVECDKSTIIKRASVPARDLRILGPVFSHSSNILAREKAIVVNLEV 121

Query: 123 IKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDVNVSRGGKW 182
           IKAIVTAEEVLLLDPLR EV+PFVE+L++   + + +           EN +  S   + 
Sbjct: 122 IKAIVTAEEVLLLDPLRPEVLPFVERLKQQFPQRNGN-----------ENALQASANVQS 181

Query: 183 LPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARNVSTKNLE 242
               EA EG Q ELPFEFQVLE ALEVVC ++D SVA LE +A+PVLDEL +NVST+NLE
Sbjct: 182 PLDPEAAEGLQSELPFEFQVLEIALEVVCSFVDKSVAALETEAWPVLDELTKNVSTENLE 241

Query: 243 RVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALLGGLASNST 302
            VRSLKSNLTR+LARVQKVRDELEHLLDDNEDMA LYLTRKW+QNQQ EA+L G ASNS 
Sbjct: 242 YVRSLKSNLTRLLARVQKVRDELEHLLDDNEDMADLYLTRKWIQNQQTEAILAGTASNS- 301

Query: 303 TAIVPH----LRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSVREY 362
            A+  H    L RL+S RS S+VTSN  + +DVEDLEMLLEAYFMQLDG RN+IL+VREY
Sbjct: 302 IALPAHNTSNLHRLTSNRSASMVTSN-TEEDDVEDLEMLLEAYFMQLDGMRNKILTVREY 361

Query: 363 IDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYDTEGIFEY 422
           IDDTEDYVNIQLDNQRNELIQ QLTLTIASFAIA ETL+A  FGMNI C LY   G+F Y
Sbjct: 362 IDDTEDYVNIQLDNQRNELIQLQLTLTIASFAIAAETLLASLFGMNIPCPLYSIHGVFGY 421

Query: 423 LVGGTTAGCLLIFVLIIGYARWKKLLGS 447
            V   TA C+++F++ +GYARWKKLLGS
Sbjct: 422 FVWSVTALCIVLFMVTLGYARWKKLLGS 436

BLAST of Sed0019853 vs. TAIR 10
Match: AT4G28580.1 (magnesium transport 5 )

HSP 1 Score: 335.1 bits (858), Expect = 8.6e-92
Identity = 206/437 (47.14%), Postives = 277/437 (63.39%), Query Frame = 0

Query: 8   SFRRSSSRRRPKKVVQSSPLSSPQPTPRIGASSPPTNSNGSSGAPVGKVKKKAGGARLWM 67
           S RRS+S R  KK         P       + SPPT    +     GK KK+ GG  LW 
Sbjct: 3   SLRRSTSNRSRKKGTAVKMNRMP------SSLSPPTPPCSAIVGGTGKSKKRRGGVCLWT 62

Query: 68  RLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKAMVVNLEFIKAIV 127
           R DR G  EV   DK+ II R+++ A+DLR     FSHSS ILAREKA+V+NLE IKA++
Sbjct: 63  RFDRTGFMEVAGCDKSTIIERSSVSAKDLR---TAFSHSSKILAREKAIVLNLEVIKAVI 122

Query: 128 TAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDVNVSRGGKWLPVSE 187
           T+E+V+LLD LR EV+   ++L+           H     D  EN +  S  G       
Sbjct: 123 TSEQVMLLDSLRPEVLTLTDRLK-----------HHFPRKDGPENILQASSHGH---QEG 182

Query: 188 AVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARNVSTKNLERVRSL 247
             EG + +LPFEF+VLE A EV C ++DS+V DLE  A+ +LDEL + VS +NL+ +RSL
Sbjct: 183 GEEGLKSKLPFEFRVLEIAFEVFCSFVDSNVVDLETQAWSILDELTKKVSNENLKDLRSL 242

Query: 248 KSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALLGGLASNSTTAIVP 307
           K++LT +LARVQKVRDE+EH LDD EDM  LYLTRKW+QNQQ EA     ASNS  +  P
Sbjct: 243 KTSLTHLLARVQKVRDEIEHFLDDKEDMEDLYLTRKWIQNQQTEA-----ASNSIVS-QP 302

Query: 308 HLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSVREYIDDTEDYVN 367
           +L+R +S R   + TS + + +D++D+EMLLEAYFMQL+G RN+IL ++E+ID TE YV 
Sbjct: 303 NLQRHTSNR---ISTSMVTEEDDIDDMEMLLEAYFMQLEGMRNKILLMKEHIDSTEAYVK 362

Query: 368 IQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYDTEGIFEYLVGGTTAGC 427
           I  +++RN LI   + + I ++AI   T++   FGMNI   LY T  IF Y+V    A C
Sbjct: 363 ILQNSRRNGLIHLMMLVNIGNYAITAGTVVVNLFGMNIPIGLYSTPDIFGYVVWAVVALC 407

Query: 428 LLIFVLIIGYARWKKLL 445
           +++F++ +GYA+WKKLL
Sbjct: 423 IVLFIVTVGYAKWKKLL 407

BLAST of Sed0019853 vs. TAIR 10
Match: AT5G64560.1 (magnesium transporter 9 )

HSP 1 Score: 323.2 bits (827), Expect = 3.4e-88
Identity = 193/392 (49.23%), Postives = 258/392 (65.82%), Query Frame = 0

Query: 56  VKKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKA 115
           VKKK   A  W  +D  GQSE L+ DK  I+ R  I ARDLRIL P  S+ S IL RE+A
Sbjct: 18  VKKKTPQAS-WALIDATGQSEPLDVDKYEIMHRVQIHARDLRILDPNLSYPSTILGRERA 77

Query: 116 MVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQLRKLLAKNSSSQVHGDGTVDERENDVN 175
           +V+NLE IKAI+T+EEVLL DP  + VIP VE+LR+ L   ++S   G G   E     N
Sbjct: 78  IVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELRRRLPVGNASHNGGQGDGKEIAGAQN 137

Query: 176 VSRGGKWLPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYPVLDELARN 235
                      +   G++ E PFEF+ LE ALE +C +L +  A+LE  AYP LDEL   
Sbjct: 138 -----------DGDTGDEDESPFEFRALEVALEAICSFLAARTAELETAAYPALDELTSK 197

Query: 236 VSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQNQQAEALLG 295
           +S++NL+RVR LKS +TR+ ARVQKVRDELE LLDD++DMA LYL+RK        + +G
Sbjct: 198 ISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLSRKLSSASSPISSIG 257

Query: 296 GLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDGTRNRILSV 355
                +T+  +    ++S     S+ T +  D NDVE+LEMLLEAYFMQ+D T NR+ ++
Sbjct: 258 EPNWYTTSPTIG--SKISRASRASLATVH-GDENDVEELEMLLEAYFMQIDSTLNRLTTL 317

Query: 356 REYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHCTLYDTEG- 415
           REYIDDTEDY+NIQLDN RN+LIQ +L L+  +  +++ +L+AG FGMNI  T  D  G 
Sbjct: 318 REYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAGIFGMNIPYTWNDGHGY 377

Query: 416 IFEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           +F+Y+VG T   C+++FV+I+ YAR+K L+GS
Sbjct: 378 MFKYVVGLTGTLCVVVFVIIMSYARYKGLVGS 394

BLAST of Sed0019853 vs. TAIR 10
Match: AT5G09690.1 (magnesium transporter 7 )

HSP 1 Score: 310.8 bits (795), Expect = 1.7e-84
Identity = 187/400 (46.75%), Postives = 254/400 (63.50%), Query Frame = 0

Query: 49  SGAPVGKVKKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSN 108
           S A V   +K +  +R W+ +D  GQ  VL+ DK+ I+ R  I ARDLRIL P   + S 
Sbjct: 12  SSAVVTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDLRILDPNLFYPSA 71

Query: 109 ILAREKAMVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQL-RKLLAKNSSSQVHGDGTV 168
           IL RE+A+V+NLE IKAI+TAEEVL+ D   + VIP +E+  R+L   N +  VHGDG +
Sbjct: 72  ILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVGNEAHGVHGDGDL 131

Query: 169 DERENDVNVSRGGKWLPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYP 228
                                  GE+ E PFEF+ LE ALE +C +L +   +LE+ AYP
Sbjct: 132 -----------------------GEEDESPFEFRALEVALEAICSFLAARTTELEKFAYP 191

Query: 229 VLDELARNVSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQN 288
            LDEL   +S++NLERVR LKS +TR+ ARVQKVRDELE LLDD+ DMA LYLTRK +  
Sbjct: 192 ALDELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGA 251

Query: 289 QQAEALLGGLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDG 348
             + ++        T+  +  +  +S     S+VT    D  DVE+LEMLLEAYFMQ+D 
Sbjct: 252 SSSVSVSDEPIWYPTSPTIGSM--ISRASRVSLVTVRGDDETDVEELEMLLEAYFMQIDS 311

Query: 349 TRNRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHC 408
           T N++  +REYIDDTEDY+NIQLDN RN+LIQ +L L+  +  ++V ++IAG FGMNI  
Sbjct: 312 TLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPN 371

Query: 409 TLYDTEG-IFEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           T     G IF+++V  T   C+++FV+I+ YAR++ L+GS
Sbjct: 372 TWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 386

BLAST of Sed0019853 vs. TAIR 10
Match: AT5G09690.2 (magnesium transporter 7 )

HSP 1 Score: 310.8 bits (795), Expect = 1.7e-84
Identity = 187/400 (46.75%), Postives = 254/400 (63.50%), Query Frame = 0

Query: 49  SGAPVGKVKKKAGGARLWMRLDRWGQSEVLEWDKNAIIRRAAIPARDLRILGPVFSHSSN 108
           S A V   +K +  +R W+ +D  GQ  VL+ DK+ I+ R  I ARDLRIL P   + S 
Sbjct: 23  SSAVVTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDLRILDPNLFYPSA 82

Query: 109 ILAREKAMVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVEQL-RKLLAKNSSSQVHGDGTV 168
           IL RE+A+V+NLE IKAI+TAEEVL+ D   + VIP +E+  R+L   N +  VHGDG +
Sbjct: 83  ILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVGNEAHGVHGDGDL 142

Query: 169 DERENDVNVSRGGKWLPVSEAVEGEQYELPFEFQVLESALEVVCLYLDSSVADLERDAYP 228
                                  GE+ E PFEF+ LE ALE +C +L +   +LE+ AYP
Sbjct: 143 -----------------------GEEDESPFEFRALEVALEAICSFLAARTTELEKFAYP 202

Query: 229 VLDELARNVSTKNLERVRSLKSNLTRILARVQKVRDELEHLLDDNEDMAQLYLTRKWMQN 288
            LDEL   +S++NLERVR LKS +TR+ ARVQKVRDELE LLDD+ DMA LYLTRK +  
Sbjct: 203 ALDELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGA 262

Query: 289 QQAEALLGGLASNSTTAIVPHLRRLSSVRSGSVVTSNLLDYNDVEDLEMLLEAYFMQLDG 348
             + ++        T+  +  +  +S     S+VT    D  DVE+LEMLLEAYFMQ+D 
Sbjct: 263 SSSVSVSDEPIWYPTSPTIGSM--ISRASRVSLVTVRGDDETDVEELEMLLEAYFMQIDS 322

Query: 349 TRNRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGWFGMNIHC 408
           T N++  +REYIDDTEDY+NIQLDN RN+LIQ +L L+  +  ++V ++IAG FGMNI  
Sbjct: 323 TLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPN 382

Query: 409 TLYDTEG-IFEYLVGGTTAGCLLIFVLIIGYARWKKLLGS 447
           T     G IF+++V  T   C+++FV+I+ YAR++ L+GS
Sbjct: 383 TWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 397