Sed0019762 (gene) Chayote v1

Overview
NameSed0019762
Typegene
OrganismSechium edule (Chayote v1)
Descriptionvacuolar protein sorting-associated protein 9A-like
LocationLG03: 5157872 .. 5163549 (-)
RNA-Seq ExpressionSed0019762
SyntenySed0019762
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCGAGGGTTGCAGGTGTCGTCTTCTAGCTGAGAATTTTGTGCTCTCAGAGATTTTGGAGCTCAAAGATCTTCGATCAGTGAAGGATCGATAAATCGCAGAAGAATTATAAACGTCCGGCGAGAAATGGAGAACGCCGACGTATTTTTAGGTTTGCACGACTTTCTCGAGCGCATGCGTCAACCATCGGCCTCAGATTTCGTCAAATCCATCAAAAGGTAAGTTCGTTTTCAATTGCGAACGATATTTGAAAATTATAGGATGTTTATTGACTTTAATTGGTGTTCTTGTGATCTTTCCTTTCTTGTTGTCATCTGATTTCTTTATGCGTTATTTATAGAATGGTTGCTCGTTATTTCATTTGTCTGGACCTCAAATCTCTTGCTATTTGATTCTGCAATGATTGTGCTCGTTTGCTTAATTACTATTCAAATTACTCTGCTGCTGGAGAAAATTTTTGTAGTCATCAATGGAAGAAGTAAAGTTTAACATATGAATTAGCTTTCGATCACGCATTCTGGTTTGGGACTCCATTTTTACTTCTTTCAGCTGGCACAAACCCCTCACGATGATTAGAAACGCCTATGAAGAATTTCATATGGGCATAGATTACTATATTTCTGGCAGTATGAGTCTCCACATATTCAAGGGACATTGGAAGAATATTGATAAGGCGAGATCTATTGTTTTACAACAATGTGACTAGCCGAGTTTGAAGTGGAGATTCGACTCTGTTTAGGGAAGACCAATGGGCTGAATCCAGTAAGTTAAAAGATCCTTATTCCCATCTTTACCATTTGTCCATAAAGAAACGAAGTATAATTAAATGCTTCTGGAATACTAACTCCATGGCTTGGGATCTGGCTTTGAGAAGGAAATTCACTGCATTTGAGATTGAATAATGGTCCTCTCTTTCAAACTACCTGGCTTATATTGATCTAATCGATCAAGATGTTAGATGGGTTTGGATTTTTTTTTTTTTAACTGAGAAATCAGAGCTTCGCTCCACTATACTCGGGATAAACACGCACGCCTATCCCTAGGTTAGGCACCGAGAGACATCGAAGGAATGGAGACATCGAAGGCATTTACAAGTTAATGCCGACCTCGAGGAAAGCACATCTTCAGAACCCAAGTCTTCGACCACCACGCCATCCCTTGGGGATAGATGGGTTTTGAAATTGCTAGCAAATGGAATCACAAGATCATCTCTTTAATGGATTTCTCATTGCATCTAATTTCTGGGACAGTATGCTTAACAAATTTGGCTGGTCTACAGCTGGACTTAAAGACAATAGACATTTTCTCTCTATTTTTTACCTTATGCTGCATGGCCATCCATCTCGTTAAAGGATAAAAAAGGACATTCATGGGTGAATTTTATCTTGCCTTTTTTATTTTTTATTTTTGAAATGGTGGCTTGAAAGAAACAATATGATTTTCAACAACACGCAACATGCCTTGATGTTCTTATAAAATCTGTTACTTATTTTGCTTTACTATGGATCCATTGCTTCTCCTTTTTGTAATTATGATCTCTCCCCTTTGATTTTTTTTCTTTTAAAACAAGGGTAACCACGTCCGTCCCAATCTCTCCCCTTTGATTTCTCAGTGGCAAAAATTTTTGTAACTAACTATGGTAGGACTTTTGTAATTTTTCATACATCAATAAAAAGCTTGTGTTTCCTAAAATAAAAGGAAAAAAAAGAAAATCTTCCAAGACAAAAAATCTCCATACTATTTTTTTGAATCTATAGTTTTTTTTATCGTTTATTGGTGTAGTAATCAGTCTCCTCTTATAGCCTTACCACCCTCCTTATGAATTGGAGAGAACTTTTGTAAATCCCCCTTGAGCAGTGTTTTAAAAAGCGCAAGGCGCACTAAGGCGCAATGGCCCTCTGGAGCCTGAGGCGCAAGGCGCACCAAAAGGCGAGGGCTTTTTTACTTAAGGCGCACTATATAAAAAAACACACAAAATTTATATATATGTGTGTATACACAATAAAAACACTTTGCATGGACATAAATGAAGTTTTAACCAAGAATAATATATAAAAACATACATAATATTCAACTATCAACCTTCACGCGCTTCGAATGACCTAGTACGGGGATCTGAAATACAAAAAGCGCAAAGGAAAATTAAAAAGCCCACGAGGCGCGCGCCTTTTGGCGCCTGGGGCTTCACGTAAGGCGCCATAAGGCGCGCGCCTTATTATACGCGCCTGGCCTGGGACAATTAAGGCGCAAAAGGTGGGACTTGAGCCTAGGCGCGCCTAGGCGCTCGCCTAGGCGCGCCTTTTAAAACACTGCCCTTGAGGTTGTCCCCTTCTTTTGCAATTTCATGAGCTACACATTGATTATTGATTTTGAACAATAATAAACCTATGCACAACGTTTTGACTGAACACTTTATACTGGCAGCTTTATTGTATTATTTTCGAACAATACTCCTGACGCTGAAAGAGATAGTGCTTCTGTACAAGAATTCTATGCCAAAATGGAGGCAGCTTTCAGAGTTCATCCACTGTGGTCGGGTTGCTCAGAAGAAGACCTGGAAAGTGCTGGTGAGGTAAGTTCACTACTTTTGAAGATGTATAAATATTTTATTGAATAATTGTTTGATTTCACCTCGACAATGAGAATGAGGTGAATAATTAAATTTCAATGGGTAAACGATGTGGAAAAAGAGGCTTTAGTGTTAATGATCCCATCAAGTTAACAAATTAATGCATTTGATAATGATCCATTTGATAATAATTAAATTATGTATAATCCATTTGATAATGATCCCATCAAGTTAACAAATTAATGCTATAAAAAAGTCAAGCTTTTTTTTATCTCGGTGCATATCTTTGAGAATATTCTGCAAATAGTCTTCCTGACGGGATTATATATTGTGCAAACCTACAATAACACACACTTTGCACTCCTTTAACTGATATTTAATATTTATAACATTTATTCTCTCTTCTAATGCACACCTGTCTATTCAATGCAGGGATTGGAGAAGTATGTCATGACAAAATTATATTCTCGTGTATATGCTTCACTTGCAGATGATGTAAAAATTGACGAACGACTTTCTGAAAAAATGGCATTGATACAACAGTTCATTAGGCCAGAAAATTTGGATATCAAACCAAATTTCCAGAACGAAACATCTTGGTTGGTGAGTAAAGGTTGAAGTCAAGAGATCATAGTAATCATCTCCTCTCATAAAATTTAGATCCTCATGATATTTGTTTGTGCTTAAAAATTGTACTTCAAAGTTTGTTATTTCGATACATTTTGCGAAAATTTTAAATTTAAAGAACTGTTCATCAGTTCAAATCTGGAAGCTAATCGATACTTTGTCCAGTGAAGTTTAACAGAAATTTGTAGAATATCTTCTCTTCATTAGCTTATCATCTATAAAACGTTTGCATGATTGATCTTTTCTATTTAGGCATTCAAGTATACGTTAAAAATGTCTCAAAGGTGAACTTTCTATATAGCCTAAATGTTCTCTACTTTTTAGCAATGCCAGTATAGATGGACTGTTGGAGAACTTATTATCGGATTGGCTCATGCATATTCATAATGGAAAGGAAATAAGTTGAGATGGACACAAGACAAACATTAAAACGTTATATATTTACTTTTACTGATTATGAAGTCCCCTTGAGTTCTGTAATGATAGGCCTGATCAAAACTTCTCTTATTAAACCCCCTAGGTTCTGGACAATTCTCTAGTAAAGTTTTTGGTTTTCCATGGGAATGTTATGGGAGAAAGGAAATGAAATACAGAACTTTTTCAGGCATTCTTGTTATGAACATAGTTTCTTTCACTTAGGCTGGTTCTTACTCTTCTTTGCTTAAATGTTTGTTTTCAGCAAGTCTTGTCTTTTGTAACTGAAACTTTCTTGTCTTTTACCAGTATGTCCAATTTTCTACCCTCTGAATTTTTGTTGGTCATTAATTCAAGTCTCACCTACATGTTAACATTTTTATTTGCAGCTTGCTCAGAAAGAACTCCTGAAGATTAACATGTACAAAGCTCCAAGAGACAAGCTTGTGTGTCTCCTCGGTTGTTGTAAGGTCATCAGTAACCTGCTAATTAATGCTTCTATTGCTTCAAATGAGAATCCTCCAGGGGCTGATGAGTTCCTTCCCGTCCTCATTTATGTTATCATAAAGGTGTTGTATAATATGATTACTAAAAGTTAGTTTGTTCATATATAACAATGTGATTACTCATGAAACAACTTTTTCATGCAAGAGCATGCTTTCTCATAGAATGTCATCAACTTACTCTAATAATCTCTGTTTGTCATTAAACAGAAAGCTTGTGGCAAACACCATATGATGTTCTCTATTTCATTTTTTAGGACATAATTTTCGGTAATATAAAATGTAGAGATTTAAAACATCGTGCTCATAAGTGTGTTTTTCTTGCACAATTTCTTGTAGGCAAACCCTCCACAACTGCACTCAAATTTACTGTATATACAACGATACAGGGGACAATCCCGATTAACTGGGGAAGCAGCATATTTTTTCACAAACATACTCTCTGCCGAGTCCTTCATCTCAAACATTGACGCAAATGCCCTCTCCATGGAAGAAAGTGAGTTCGAAGAAAACATGGAATCCGCTCGAGCACTCCTATCTGGTCTCTCTTCTGAAGTTGAGTCTCCTTCTGATAAAAGTGATGAAGGGGTTGCCCGTAATCCAGAACTTATAGAAACGAGTTCTCGAGCTTCAAATTCTGGGACCGAACCCTCGGTTAGACCCAAATCCGCCGAAGCAAAGCTCAGAACTGAGGTACCACATGCCAAAGATCAATCAACGGTAGTGGAAGTTCCAACGCTCTCAGAGTTGGAAAACAAGGGTGCAGCTATGCTTTTAAATGATCACACAGGGAAGCAAGCCTTCCAAGATTATCCATATTTGTTTGCTCAAGTTGGAGACCTTACATTTAACGACGTAGAAGAGCTACTCAACCAATACAAACGACTAGTTTTCAAATACGTTTCCCTTTCTAAAGGATTGAGTCTCACTTCTTCATCCGCATCTTTGTCAAATTCTGAAATACATTCCCACCATAATCCTGAATCTTCAAAAGAACCTGAAGATGTTAGAGACGTGACATTGAAGTACGAGTCAACTGAAGAGACAGATACGACAGACAACGTCGGATCGGGGTTACTGCAAGACAAGATAGTACTACCTCAACATGAAGGAAACGGCAAGACATCACTATCCTAGCACCTTCAAGAACTTTTATGTGACCAATCAACCAAATGTGTTCAGTCTGGTTATCTTGCTTTGCTAGCAGTAAACAATTGTCATGTCTAGGATCAGATCGCAGGCCGATGATGGGCATGGAAGTGAAGATCAGAAATGAGAATTTAGCCTTGATTTTGGATCTGGCAATGAACAGGAAATCAAGTCCCTAAGAAAACAAACTATTGGCGGCTTGCATCCACAAGTACGTTCTCTATCCCCCCCTGTAACATTCTAGTAGCTTCATTCAGGAGCATATTGATAATTTCATAGATTGGAAAGACCAAACTTTGTCAATATAAGTTATTAATCTACAATTCTTATGCTATTGGACATTTTTTTAACATAATGCTGAACCAATCTCTGATTCTCATGCATGGATATAACTTTTATTCAATAAGACAGAGCTGG

mRNA sequence

GTCGAGGGTTGCAGGTGTCGTCTTCTAGCTGAGAATTTTGTGCTCTCAGAGATTTTGGAGCTCAAAGATCTTCGATCAGTGAAGGATCGATAAATCGCAGAAGAATTATAAACGTCCGGCGAGAAATGGAGAACGCCGACGTATTTTTAGGTTTGCACGACTTTCTCGAGCGCATGCGTCAACCATCGGCCTCAGATTTCGTCAAATCCATCAAAAGCTTTATTGTATTATTTTCGAACAATACTCCTGACGCTGAAAGAGATAGTGCTTCTGTACAAGAATTCTATGCCAAAATGGAGGCAGCTTTCAGAGTTCATCCACTGTGGTCGGGTTGCTCAGAAGAAGACCTGGAAAGTGCTGGTGAGGGATTGGAGAAGTATGTCATGACAAAATTATATTCTCGTGTATATGCTTCACTTGCAGATGATGTAAAAATTGACGAACGACTTTCTGAAAAAATGGCATTGATACAACAGTTCATTAGGCCAGAAAATTTGGATATCAAACCAAATTTCCAGAACGAAACATCTTGGTTGCTTGCTCAGAAAGAACTCCTGAAGATTAACATGTACAAAGCTCCAAGAGACAAGCTTGTGTGTCTCCTCGGTTGTTGTAAGGTCATCAGTAACCTGCTAATTAATGCTTCTATTGCTTCAAATGAGAATCCTCCAGGGGCTGATGAGTTCCTTCCCGTCCTCATTTATGTTATCATAAAGGCAAACCCTCCACAACTGCACTCAAATTTACTGTATATACAACGATACAGGGGACAATCCCGATTAACTGGGGAAGCAGCATATTTTTTCACAAACATACTCTCTGCCGAGTCCTTCATCTCAAACATTGACGCAAATGCCCTCTCCATGGAAGAAAGTGAGTTCGAAGAAAACATGGAATCCGCTCGAGCACTCCTATCTGGTCTCTCTTCTGAAGTTGAGTCTCCTTCTGATAAAAGTGATGAAGGGGTTGCCCGTAATCCAGAACTTATAGAAACGAGTTCTCGAGCTTCAAATTCTGGGACCGAACCCTCGGTTAGACCCAAATCCGCCGAAGCAAAGCTCAGAACTGAGGTACCACATGCCAAAGATCAATCAACGGTAGTGGAAGTTCCAACGCTCTCAGAGTTGGAAAACAAGGGTGCAGCTATGCTTTTAAATGATCACACAGGGAAGCAAGCCTTCCAAGATTATCCATATTTGTTTGCTCAAGTTGGAGACCTTACATTTAACGACGTAGAAGAGCTACTCAACCAATACAAACGACTAGTTTTCAAATACGTTTCCCTTTCTAAAGGATTGAGTCTCACTTCTTCATCCGCATCTTTGTCAAATTCTGAAATACATTCCCACCATAATCCTGAATCTTCAAAAGAACCTGAAGATGTTAGAGACGTGACATTGAAGTACGAGTCAACTGAAGAGACAGATACGACAGACAACGTCGGATCGGGGTTACTGCAAGACAAGATAGTACTACCTCAACATGAAGGAAACGGCAAGACATCACTATCCTAGCACCTTCAAGAACTTTTATGTGACCAATCAACCAAATGTGTTCAGTCTGGTTATCTTGCTTTGCTAGCAGTAAACAATTGTCATGTCTAGGATCAGATCGCAGGCCGATGATGGGCATGGAAGTGAAGATCAGAAATGAGAATTTAGCCTTGATTTTGGATCTGGCAATGAACAGGAAATCAAGTCCCTAAGAAAACAAACTATTGGCGGCTTGCATCCACAAGTACGTTCTCTATCCCCCCCTGTAACATTCTAGTAGCTTCATTCAGGAGCATATTGATAATTTCATAGATTGGAAAGACCAAACTTTGTCAATATAAGTTATTAATCTACAATTCTTATGCTATTGGACATTTTTTTAACATAATGCTGAACCAATCTCTGATTCTCATGCATGGATATAACTTTTATTCAATAAGACAGAGCTGG

Coding sequence (CDS)

ATGGAGAACGCCGACGTATTTTTAGGTTTGCACGACTTTCTCGAGCGCATGCGTCAACCATCGGCCTCAGATTTCGTCAAATCCATCAAAAGCTTTATTGTATTATTTTCGAACAATACTCCTGACGCTGAAAGAGATAGTGCTTCTGTACAAGAATTCTATGCCAAAATGGAGGCAGCTTTCAGAGTTCATCCACTGTGGTCGGGTTGCTCAGAAGAAGACCTGGAAAGTGCTGGTGAGGGATTGGAGAAGTATGTCATGACAAAATTATATTCTCGTGTATATGCTTCACTTGCAGATGATGTAAAAATTGACGAACGACTTTCTGAAAAAATGGCATTGATACAACAGTTCATTAGGCCAGAAAATTTGGATATCAAACCAAATTTCCAGAACGAAACATCTTGGTTGCTTGCTCAGAAAGAACTCCTGAAGATTAACATGTACAAAGCTCCAAGAGACAAGCTTGTGTGTCTCCTCGGTTGTTGTAAGGTCATCAGTAACCTGCTAATTAATGCTTCTATTGCTTCAAATGAGAATCCTCCAGGGGCTGATGAGTTCCTTCCCGTCCTCATTTATGTTATCATAAAGGCAAACCCTCCACAACTGCACTCAAATTTACTGTATATACAACGATACAGGGGACAATCCCGATTAACTGGGGAAGCAGCATATTTTTTCACAAACATACTCTCTGCCGAGTCCTTCATCTCAAACATTGACGCAAATGCCCTCTCCATGGAAGAAAGTGAGTTCGAAGAAAACATGGAATCCGCTCGAGCACTCCTATCTGGTCTCTCTTCTGAAGTTGAGTCTCCTTCTGATAAAAGTGATGAAGGGGTTGCCCGTAATCCAGAACTTATAGAAACGAGTTCTCGAGCTTCAAATTCTGGGACCGAACCCTCGGTTAGACCCAAATCCGCCGAAGCAAAGCTCAGAACTGAGGTACCACATGCCAAAGATCAATCAACGGTAGTGGAAGTTCCAACGCTCTCAGAGTTGGAAAACAAGGGTGCAGCTATGCTTTTAAATGATCACACAGGGAAGCAAGCCTTCCAAGATTATCCATATTTGTTTGCTCAAGTTGGAGACCTTACATTTAACGACGTAGAAGAGCTACTCAACCAATACAAACGACTAGTTTTCAAATACGTTTCCCTTTCTAAAGGATTGAGTCTCACTTCTTCATCCGCATCTTTGTCAAATTCTGAAATACATTCCCACCATAATCCTGAATCTTCAAAAGAACCTGAAGATGTTAGAGACGTGACATTGAAGTACGAGTCAACTGAAGAGACAGATACGACAGACAACGTCGGATCGGGGTTACTGCAAGACAAGATAGTACTACCTCAACATGAAGGAAACGGCAAGACATCACTATCCTAG

Protein sequence

MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAAFRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIRPENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNIDANALSMEESEFEENMESARALLSGLSSEVESPSDKSDEGVARNPELIETSSRASNSGTEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHTGKQAFQDYPYLFAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEPEDVRDVTLKYESTEETDTTDNVGSGLLQDKIVLPQHEGNGKTSLS
Homology
BLAST of Sed0019762 vs. NCBI nr
Match: XP_023525567.1 (vacuolar protein sorting-associated protein 9A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 772.3 bits (1993), Expect = 2.3e-219
Identity = 408/476 (85.71%), Postives = 431/476 (90.55%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIV FSNN PD ERDSASVQEF+AKME A
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+LESAGEGLEKYVMTKLYSRVYASLADDVKIDE+LS+KMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLL+NASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSEVESPSDKS--DEGVARNPELIETSSRASNSG 300
           DANALSMEE+EFE+NMESARALLSGLS++VES S+KS  DE VARN EL+ETSS+ASNSG
Sbjct: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHTGKQAFQDYPYL 360
           TEP+VRP S E+K  TE PH K QSTV+EV TLS+LENKGAAMLLNDHT KQAF+DYPYL
Sbjct: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEPE 420
           FAQVGDLTFNDVEELLNQY+ LVFKYVSLSKGLSL SSS S SNSE+HSHHNPES KEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPE 420

Query: 421 DVRDVTLKYESTEETDT------------TDNVGSGLLQDKIVLPQHEGNGKTSLS 463
           DVRDVT   ES  ET T             +NVGSGL+QDK+VLPQHEGN  TSLS
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476

BLAST of Sed0019762 vs. NCBI nr
Match: XP_022955656.1 (vacuolar protein sorting-associated protein 9A-like [Cucurbita moschata] >XP_022955669.1 vacuolar protein sorting-associated protein 9A-like [Cucurbita moschata])

HSP 1 Score: 763.8 bits (1971), Expect = 8.3e-217
Identity = 406/476 (85.29%), Postives = 428/476 (89.92%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIV FSNN PD ERDSASVQEF+AKME A
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+LESAGEGLEKYVMTKLYSRVYASLADDVKIDE+LS+KMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLL+NASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSEVESPSDKS--DEGVARNPELIETSSRASNSG 300
           DANALSMEE+EFE+NMESARALLSGLS++VES S+KS  DE VARN EL+ETSS+ASNSG
Sbjct: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHTGKQAFQDYPYL 360
           TEP+VRP S E+K  TE PH K QSTV EV TLS+LENKGAAMLLNDHT KQAF+DYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVTEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEPE 420
           FAQVGDLTFNDVEELLNQY+ LVFKYVSLSKGLSL SSS S SNSE+HSH NPES KEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHLNPESFKEPE 420

Query: 421 DVRDVTLKYESTEETDT------------TDNVGSGLLQDKIVLPQHEGNGKTSLS 463
           DVRDVT   ES  ET T             +NVGSGL+QDK+VLPQHE N  TSLS
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEVNDTTSLS 476

BLAST of Sed0019762 vs. NCBI nr
Match: XP_022990551.1 (vacuolar protein sorting-associated protein 9A-like [Cucurbita maxima] >XP_022990552.1 vacuolar protein sorting-associated protein 9A-like [Cucurbita maxima])

HSP 1 Score: 761.1 bits (1964), Expect = 5.4e-216
Identity = 402/476 (84.45%), Postives = 429/476 (90.13%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIV FSNN PD ERD ASVQEF+AKME A
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDGASVQEFFAKMEGA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+LESAGEGLEKYVMTKLYSRVYASLADDVKIDE+LS+KMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLL+NASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 181 SPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSEVESPSDKS--DEGVARNPELIETSSRASNSG 300
           DAN+LSMEE+EFE+NMESARALLSGLS++VES S+KS  DE VARN EL+ETSS+ASNSG
Sbjct: 241 DANSLSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHTGKQAFQDYPYL 360
           TEP+VRP S E+K  TE PH K QSTV+EV TLS+LE+KGAAMLLNDHT KQAF+DYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVMEVSTLSDLESKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEPE 420
           FAQVGDLTFNDVEELLNQY+ +VFKYVSLSKGLSL SSS S S+SE+HSHHNPES KEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHVVFKYVSLSKGLSLRSSSPSSSSSEMHSHHNPESFKEPE 420

Query: 421 DVRDVTLKYESTEETDT------------TDNVGSGLLQDKIVLPQHEGNGKTSLS 463
           DVRDVT   ES  ET T             +NVGSGL+QDK+VLPQHEGN  TSLS
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSLFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476

BLAST of Sed0019762 vs. NCBI nr
Match: KAG7032750.1 (Vacuolar protein sorting-associated protein 9A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 748.8 bits (1932), Expect = 2.8e-212
Identity = 405/500 (81.00%), Postives = 427/500 (85.40%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKS------------------------FIVLF 60
           MENADVFLGLHDFLERMRQPSASDFVKSIK                         FIV F
Sbjct: 24  MENADVFLGLHDFLERMRQPSASDFVKSIKRCAYLYVYALVCRVSDRYAFCSLILFIVSF 83

Query: 61  SNNTPDAERDSASVQEFYAKMEAAFRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYA 120
           SNN PD ERDSASVQEF+AKME AFR HPLWSGCSEE+LESAGEGLEKYVMTKLYSRVYA
Sbjct: 84  SNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYA 143

Query: 121 SLADDVKIDERLSEKMALIQQFIRPENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKL 180
           SLADDVKIDE+LS+KMALIQQFIRPENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKL
Sbjct: 144 SLADDVKIDEQLSDKMALIQQFIRPENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKL 203

Query: 181 VCLLGCCKVISNLLINASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ 240
           VCLLGCCKVISNLL+NASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ
Sbjct: 204 VCLLGCCKVISNLLLNASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ 263

Query: 241 SRLTGEAAYFFTNILSAESFISNIDANALSMEESEFEENMESARALLSGLSSEVESPSDK 300
           SRLTGEAAYFFTNILSAESFISNIDANALSMEE+EFE+NMESARALLSGLS++VES S+K
Sbjct: 264 SRLTGEAAYFFTNILSAESFISNIDANALSMEETEFEKNMESARALLSGLSTDVESSSNK 323

Query: 301 S--DEGVARNPELIETSSRASNSGTEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSEL 360
           S  DE VA N EL+ETSS+ASNSGTEP+VRP S E+K  TE PH K QSTV EV TLS+L
Sbjct: 324 SNLDERVACNSELVETSSQASNSGTEPAVRPNSPESKPSTEGPHVKYQSTVTEVSTLSDL 383

Query: 361 ENKGAAMLLNDHTGKQAFQDYPYLFAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLT 420
           ENKGAAMLLNDHT KQAF+DYPYLFAQVGDLTFNDVEELLNQY+ LVFKYVSLSKGLSL 
Sbjct: 384 ENKGAAMLLNDHTRKQAFRDYPYLFAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLR 443

Query: 421 SSSASLSNSEIHSHHNPESSKEPEDVRDVTLKYESTEETDT------------TDNVGSG 463
           SSS S SNSE+HSH NPES KEPEDVRDVT   ES  ET T             +NVGSG
Sbjct: 444 SSSPSSSNSEMHSHLNPESFKEPEDVRDVTSNDESAGETHTMIDGPDGTSLFGEENVGSG 503

BLAST of Sed0019762 vs. NCBI nr
Match: XP_022141647.1 (vacuolar protein sorting-associated protein 9A-like [Momordica charantia])

HSP 1 Score: 723.8 bits (1867), Expect = 9.5e-205
Identity = 380/472 (80.51%), Postives = 418/472 (88.56%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIV FSNN PD ERDSASVQEF+AKME A
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+LESAGEGLEKYVMTKL++RVYASL DDVKIDE+LS+K+ALIQQF R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFAR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLL CCKVISNLLINAS+ASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSEVESPSDKS--DEGVARNPELIETSSRASNSG 300
           DA ALSM+E EFE  MESARALLSGLS++ E+ S+KS  DEGV RN  L+++SS+ASN G
Sbjct: 241 DAKALSMDEIEFENKMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300

Query: 301 TEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHTGKQAFQDYPYL 360
           TEP+V PKSAEAK RTE   AKDQSTV+E P+LS+LENKGAA+LLNDHTG+QAF+DYPYL
Sbjct: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEPE 420
           FAQVGDLTFNDV+ELL+QYK+LVFKY+SLSKGLSLT+SS S+S+SE+ SHH PES KEPE
Sbjct: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420

Query: 421 DVRDVTLKYESTEETDT------------TDNVGSGLLQDKIVLPQHEGNGK 459
            +RDVT   ES  ET               ++VGSGL QDK+VL QHEGN K
Sbjct: 421 GIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDK 472

BLAST of Sed0019762 vs. ExPASy Swiss-Prot
Match: Q9LT31 (Vacuolar protein sorting-associated protein 9A OS=Arabidopsis thaliana OX=3702 GN=VPS9A PE=1 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 3.1e-142
Identity = 280/448 (62.50%), Postives = 337/448 (75.22%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MEN DVFLGLHDFLERMR+PSA DFVKSIKSFIV FSNN PD E+D A VQEF++KMEAA
Sbjct: 1   MENTDVFLGLHDFLERMRKPSAGDFVKSIKSFIVSFSNNAPDPEKDCAMVQEFFSKMEAA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+L+SAG+GLEKYVMTKL++RV+AS  ++V  DE+L +KM+L+QQFI 
Sbjct: 61  FRAHPLWSGCSEEELDSAGDGLEKYVMTKLFTRVFASNTEEVIADEKLFQKMSLVQQFIS 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDI+P FQNE+SWLLAQKEL KINMYKAPRDKLVC+L CCKVI+NLL+NASIASNEN
Sbjct: 121 PENLDIQPTFQNESSWLLAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
            PGADEFLPVLIYV IKANPPQLHSNLLYIQRYR +S+L GEAAYFFTNILSAESFISNI
Sbjct: 181 APGADEFLPVLIYVTIKANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSE--------VESPSDKS--DEGVARNPE---- 300
           DA ++S++E+EFE+NMESARA +SGL S+           P D+S   +  + NP+    
Sbjct: 241 DAKSISLDEAEFEKNMESARARISGLDSQTYQTGHGSAPPPRDESTLQKTQSLNPKRENT 300

Query: 301 LIETSSRASNSGTEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDH 360
           L ++ S  S SGT   +        + +E P  K +S       +S+LENKGA  LL D 
Sbjct: 301 LFQSKSSDSLSGTNELL-------NINSETPMKKAES-------ISDLENKGAT-LLKDT 360

Query: 361 TGKQAFQDYPYLFAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIH 420
              + FQ+YPY+FA  GDL   DVE LLN YK+LVFKYV L+KGL    +S + S+S + 
Sbjct: 361 EPSKVFQEYPYIFASAGDLRIGDVEGLLNSYKQLVFKYVCLTKGLG-DGTSLAPSSSPLQ 420

Query: 421 SHHNPESSKEPEDVRDVTLKYESTEETD 435
           +     +SKE ED R  +   + T+ETD
Sbjct: 421 ASSGFNTSKESEDHRRSSSDVQMTKETD 432

BLAST of Sed0019762 vs. ExPASy Swiss-Prot
Match: Q10NQ3 (Vacuolar protein sorting-associated protein 9A OS=Oryza sativa subsp. japonica OX=39947 GN=VPS9A PE=1 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 9.8e-128
Identity = 251/441 (56.92%), Postives = 316/441 (71.66%), Query Frame = 0

Query: 8   LGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAAFRVHPLW 67
           L  HDFLERMRQPSA+DFVKSIK FIV FSN  PD E DSA+VQEF   ME AFR H  W
Sbjct: 17  LAWHDFLERMRQPSAADFVKSIKGFIVTFSNRAPDPEHDSAAVQEFLENMEGAFRAHTPW 76

Query: 68  SGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIRPENLDIK 127
           +G SEE+LESAGEGLEKYVMTKL++RV+AS+ +DVK DE L EKM+L+QQFIRPENLDIK
Sbjct: 77  AGSSEEELESAGEGLEKYVMTKLFNRVFASVPEDVKSDEELFEKMSLLQQFIRPENLDIK 136

Query: 128 PNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNENPPGADEF 187
           P +Q+ETSWLLAQKEL KINMYKAPRDKL C+L CCKVI+NLL+NASI SNENPPGADEF
Sbjct: 137 PEYQSETSWLLAQKELQKINMYKAPRDKLACILNCCKVINNLLLNASIVSNENPPGADEF 196

Query: 188 LPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNIDANALSM 247
           LPVLIYV IKANPPQLHSNLLYIQRYR QSRL  EA YFFTNILSAESFI NID  +LSM
Sbjct: 197 LPVLIYVTIKANPPQLHSNLLYIQRYRRQSRLVSEAQYFFTNILSAESFIWNIDGESLSM 256

Query: 248 EESEFEENMESARALLSGLSSEVESPSDKSDEGVARNPELIETSSRASNSGTEPSVRPKS 307
           +E +F++ M+ AR  + GLS+  E+  ++++  V       +T   + +S    S++   
Sbjct: 257 DERDFQKKMDLARERMLGLSASSENQDNQNNLDVREQKS--QTLKASRDSDVNLSLKDNF 316

Query: 308 AEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHTGKQAFQDYPYLFAQVGDLTF 367
               L          + V  V ++S+LE KGAA LL D    +  Q+YP+LFA+ GDLT 
Sbjct: 317 QGPGLEMRRDSDASSNPVERVQSISDLEKKGAAELLKDDDLNKKIQEYPFLFARSGDLTV 376

Query: 368 NDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEPEDVRDVTLKY 427
            DVE LLN YK+LV KYV+LS+G+ +   +  + + +  S  +   S+EP++V++     
Sbjct: 377 ADVENLLNSYKQLVLKYVALSQGMGINLENPPVQSMQTVS--DLVESEEPKNVKNAVNFS 436

Query: 428 ESTEETDTTDNVGSGLLQDKI 449
           E + +  T+D++ +  L  ++
Sbjct: 437 EGSSK--TSDDIKNDTLYSEV 451

BLAST of Sed0019762 vs. ExPASy Swiss-Prot
Match: O74396 (Vacuolar protein sorting-associated protein 9a OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vps901 PE=1 SV=3)

HSP 1 Score: 164.5 bits (415), Expect = 2.9e-39
Identity = 110/329 (33.43%), Postives = 179/329 (54.41%), Query Frame = 0

Query: 11  HDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAAFRVHPLWSGC 70
           H FLE++R  SA    K +KSF+  F+            +++F   +      +  W+  
Sbjct: 116 HMFLEQLRSSSAEPVAKYLKSFLSEFTKRRWTVNYQVKLIRDFLKFINEKIEQYEPWASG 175

Query: 71  SEEDLESAGEGLEKYVMTKLYSRVY------------ASLADDVKIDERLSEKMALIQQF 130
           S+ ++++A EG+EK V+ +LY+ ++            +  +DDV+ D  LSEKM L  Q+
Sbjct: 176 SQAEIDNAKEGMEKLVLNRLYTSLFSPEIAKSGIPLSSEHSDDVEEDRVLSEKMELF-QW 235

Query: 131 IRPENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASN 190
           I  ENLDIK    +   + LA  EL +IN Y APRDK++CLL CCKVI + L N  +   
Sbjct: 236 ITEENLDIKKQKSSSKFFKLAADELRRINDYHAPRDKIICLLNCCKVIFSYLRN--VVKE 295

Query: 191 ENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFIS 250
           E+   AD F+P+LI+V+++A P  L SN+ YIQR+R   +LTGE  Y+ + ++ A SFI 
Sbjct: 296 ES---ADMFVPILIFVVLQARPAHLVSNIQYIQRFRSPEKLTGEVMYYLSTLMGAMSFIE 355

Query: 251 NIDANALSMEESEFEENMESARALLS--GLSSEVESPSDKSDEGVARNPELIE--TSSRA 310
            +D ++L++ E EF   +E +   +    LS + ES +  ++    ++P L    +SS  
Sbjct: 356 TLDCSSLTITEEEFNAQIEKSIKKMEERKLSEKSESKTAVNENATYKDPVLSRGLSSSID 415

Query: 311 SNSGTEPSVRPKSAE--AKLRTEVPHAKD 322
            ++G      P+  E    L+ + P +K+
Sbjct: 416 VSTGVALVNLPEELENMKYLQIDTPESKE 438

BLAST of Sed0019762 vs. ExPASy Swiss-Prot
Match: O18973 (Rab5 GDP/GTP exchange factor OS=Bos taurus OX=9913 GN=RABGEF1 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 9.7e-27
Identity = 83/257 (32.30%), Postives = 139/257 (54.09%), Query Frame = 0

Query: 12  DFLERMR--QPSASDFVKSIKSFI-VLFSNNTPDAERDSASVQEFY----AKMEAAFRVH 71
           +F+E ++    +  +  K  K F+  +        E  S   Q+FY     +M+   +V 
Sbjct: 142 EFIEFLKTFHKTGQEIYKQTKLFLEAMHYKRDLSIEEQSECTQDFYQNVAERMQTRGKVP 201

Query: 72  PLWSGCSEEDLESAGEGLEKYVMTKLYSRVYA-SLADDVKIDERLSEKMALIQQFIRPEN 131
           P       E +E   + +EKY+MT+LY  V+     DD K D  + +++  +  ++ P+ 
Sbjct: 202 P-------ERVEKIMDQIEKYIMTRLYKYVFCPETTDDEKKDLAIQKRIRAL-HWVTPQM 261

Query: 132 LDIKPNFQ-NETSWLL--AQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 191
           L +  N +  E S ++  A  ++++++  + PRDKL C+  C K I N +    I  NE 
Sbjct: 262 LCVPVNEEIPEVSDMVVKAITDIIEMDSKRVPRDKLACITKCSKHIFNAI---KITKNE- 321

Query: 192 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRL-TGEAAYFFTNILSAESFISN 251
           P  AD+FLP LIY+++K NPP+L SN+ YI R+   SRL TGE  Y+FTN+  A +FI  
Sbjct: 322 PASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAVAFIEK 381

Query: 252 IDANALSMEESEFEENM 257
           +DA +L++ + +F+  M
Sbjct: 382 LDAQSLNLSQEDFDRYM 386

BLAST of Sed0019762 vs. ExPASy Swiss-Prot
Match: Q9JM13 (Rab5 GDP/GTP exchange factor OS=Mus musculus OX=10090 GN=Rabgef1 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 4.8e-26
Identity = 83/255 (32.55%), Postives = 138/255 (54.12%), Query Frame = 0

Query: 12  DFLERMRQPSASDFVKSIKSFI-VLFSNNTPDAERDSASVQEFY----AKMEAAFRVHPL 71
           DFL+   + +  +  K  K F+  +        E  S   Q+FY     +M+   +V P 
Sbjct: 144 DFLKTFHK-TGQEVYKQTKMFLEAMPYKRDLSIEEQSECTQDFYQNVAERMQTRGKVPP- 203

Query: 72  WSGCSEEDLESAGEGLEKYVMTKLYSRVYA-SLADDVKIDERLSEKMALIQQFIRPENLD 131
                 E +E   + +EK++MT+LY  V+     DD K D  + +++  +  ++ P+ L 
Sbjct: 204 ------EKVEKIMDQIEKHIMTRLYKFVFCPETTDDEKKDLAIQKRIRAL-HWVTPQMLC 263

Query: 132 IKPNFQ-NETSWLL--AQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNENPP 191
           +  N +  E S ++  A  ++++++  + PRDKL C+  C K I N +    I  NE P 
Sbjct: 264 VPVNEEIPEVSDMVVKAITDIIEMDSKRVPRDKLACITRCSKHIFNAI---KITKNE-PA 323

Query: 192 GADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRL-TGEAAYFFTNILSAESFISNID 251
            AD+FLP LIY+++K NPP+L SN+ YI R+   SRL TGE  Y+FTN+  A +FI  +D
Sbjct: 324 SADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAVAFIEKLD 383

Query: 252 ANALSMEESEFEENM 257
           A +L++ + +F+  M
Sbjct: 384 AQSLNLSQEDFDRYM 385

BLAST of Sed0019762 vs. ExPASy TrEMBL
Match: A0A6J1GWY3 (vacuolar protein sorting-associated protein 9A-like OS=Cucurbita moschata OX=3662 GN=LOC111457590 PE=4 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 4.0e-217
Identity = 406/476 (85.29%), Postives = 428/476 (89.92%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIV FSNN PD ERDSASVQEF+AKME A
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+LESAGEGLEKYVMTKLYSRVYASLADDVKIDE+LS+KMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLL+NASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSEVESPSDKS--DEGVARNPELIETSSRASNSG 300
           DANALSMEE+EFE+NMESARALLSGLS++VES S+KS  DE VARN EL+ETSS+ASNSG
Sbjct: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHTGKQAFQDYPYL 360
           TEP+VRP S E+K  TE PH K QSTV EV TLS+LENKGAAMLLNDHT KQAF+DYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVTEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEPE 420
           FAQVGDLTFNDVEELLNQY+ LVFKYVSLSKGLSL SSS S SNSE+HSH NPES KEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHLNPESFKEPE 420

Query: 421 DVRDVTLKYESTEETDT------------TDNVGSGLLQDKIVLPQHEGNGKTSLS 463
           DVRDVT   ES  ET T             +NVGSGL+QDK+VLPQHE N  TSLS
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEVNDTTSLS 476

BLAST of Sed0019762 vs. ExPASy TrEMBL
Match: A0A6J1JJ31 (vacuolar protein sorting-associated protein 9A-like OS=Cucurbita maxima OX=3661 GN=LOC111487398 PE=4 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.6e-216
Identity = 402/476 (84.45%), Postives = 429/476 (90.13%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIV FSNN PD ERD ASVQEF+AKME A
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDGASVQEFFAKMEGA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+LESAGEGLEKYVMTKLYSRVYASLADDVKIDE+LS+KMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLL+NASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 181 SPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSEVESPSDKS--DEGVARNPELIETSSRASNSG 300
           DAN+LSMEE+EFE+NMESARALLSGLS++VES S+KS  DE VARN EL+ETSS+ASNSG
Sbjct: 241 DANSLSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHTGKQAFQDYPYL 360
           TEP+VRP S E+K  TE PH K QSTV+EV TLS+LE+KGAAMLLNDHT KQAF+DYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVMEVSTLSDLESKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEPE 420
           FAQVGDLTFNDVEELLNQY+ +VFKYVSLSKGLSL SSS S S+SE+HSHHNPES KEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHVVFKYVSLSKGLSLRSSSPSSSSSEMHSHHNPESFKEPE 420

Query: 421 DVRDVTLKYESTEETDT------------TDNVGSGLLQDKIVLPQHEGNGKTSLS 463
           DVRDVT   ES  ET T             +NVGSGL+QDK+VLPQHEGN  TSLS
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSLFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476

BLAST of Sed0019762 vs. ExPASy TrEMBL
Match: A0A6J1CKF0 (vacuolar protein sorting-associated protein 9A-like OS=Momordica charantia OX=3673 GN=LOC111011952 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.6e-205
Identity = 380/472 (80.51%), Postives = 418/472 (88.56%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIV FSNN PD ERDSASVQEF+AKME A
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+LESAGEGLEKYVMTKL++RVYASL DDVKIDE+LS+K+ALIQQF R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFAR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLL CCKVISNLLINAS+ASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSEVESPSDKS--DEGVARNPELIETSSRASNSG 300
           DA ALSM+E EFE  MESARALLSGLS++ E+ S+KS  DEGV RN  L+++SS+ASN G
Sbjct: 241 DAKALSMDEIEFENKMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300

Query: 301 TEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHTGKQAFQDYPYL 360
           TEP+V PKSAEAK RTE   AKDQSTV+E P+LS+LENKGAA+LLNDHTG+QAF+DYPYL
Sbjct: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEPE 420
           FAQVGDLTFNDV+ELL+QYK+LVFKY+SLSKGLSLT+SS S+S+SE+ SHH PES KEPE
Sbjct: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420

Query: 421 DVRDVTLKYESTEETDT------------TDNVGSGLLQDKIVLPQHEGNGK 459
            +RDVT   ES  ET               ++VGSGL QDK+VL QHEGN K
Sbjct: 421 GIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDK 472

BLAST of Sed0019762 vs. ExPASy TrEMBL
Match: A0A1S3B4M4 (vacuolar protein sorting-associated protein 9A-like OS=Cucumis melo OX=3656 GN=LOC103485761 PE=4 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 2.2e-199
Identity = 382/477 (80.08%), Postives = 413/477 (86.58%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MEN DVFLGLHDFLERMRQPSASDFVKSIKSFIV FSNN PD ERDSASVQEF+AKME A
Sbjct: 29  MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 88

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+LESAGEGLEKYVMTKL+SRVYASLADDVKIDE+LSEKMALIQQFIR
Sbjct: 89  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 148

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINM+KAPRDKLVCLL CCKVISNLL NASIASNEN
Sbjct: 149 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 208

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 209 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 268

Query: 241 DANALSMEESEFEENMESARALLSGLSSEVESPSDKS--DEGVARNPELIETSSRASNSG 300
           DA ALSMEE EFE+NMESARALLSGLSS+VE+ S+K+  DEGV    EL+ETSS+AS   
Sbjct: 269 DAKALSMEEIEFEKNMESARALLSGLSSDVEAISNKNNLDEGVGHYSELVETSSQAS--- 328

Query: 301 TEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHT-GKQAFQDYPY 360
            E  VRPK  E K RTE PHAKDQSTV+EVP+LS+LENKGA +LLND T G+QA +DYPY
Sbjct: 329 VESQVRPKPTEGKPRTEAPHAKDQSTVMEVPSLSDLENKGATILLNDQTGGRQALRDYPY 388

Query: 361 LFAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEP 420
           LF+QVGDLTF DVEELL+QYK+LVFKYVSLS+GL LT+  +S SNSE++S H+ ES KEP
Sbjct: 389 LFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLGLTTELSSYSNSEMYSQHHHESFKEP 448

Query: 421 EDVRDVTLKYESTEE-------TDTT-----DNVGSGLLQDKIVLPQHEGNGKTSLS 463
           EDVRD+T   ES  E        D T     +NVGSGL  DK+VLPQHEGN + S S
Sbjct: 449 EDVRDMTSNDESAGEGHRLIDGPDGTSLFGEENVGSGL--DKVVLPQHEGNNEMSQS 500

BLAST of Sed0019762 vs. ExPASy TrEMBL
Match: A0A0A0LI50 (VPS9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G128630 PE=4 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 5.4e-198
Identity = 377/477 (79.04%), Postives = 411/477 (86.16%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MEN DVFLGLHDFLERMRQPSASDFVKSIKSFIV FSNN PD ERDSASVQEF+AKME A
Sbjct: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+LESAGEGLEKYVMTKL+SRVYASLADDVKIDE++SEKMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQISEKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINM+KAPRDKLVCLL CCKVISNLL NASIASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTN+LSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSEVESPSDKS--DEGVARNPELIETSSRASNSG 300
           DA ALSMEE EFE+NMESARALLSGLSS+VE+ S+K+  DEGV    EL+ETSS+AS   
Sbjct: 241 DAKALSMEEIEFEKNMESARALLSGLSSDVEALSNKNNLDEGVGHYSELVETSSQAS--- 300

Query: 301 TEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHT-GKQAFQDYPY 360
            E  VRPK  E K RTE PHAKDQS V++VP+LS+LENKGA +LLND T G+QA ++YPY
Sbjct: 301 VESPVRPKPTEGKPRTEAPHAKDQSAVMKVPSLSDLENKGATILLNDQTGGRQALREYPY 360

Query: 361 LFAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEP 420
           LF+QVGDLTF DVEELL+QYK+LVFKYVSLS+GLSLT+  +S S SE+HS H+ ES KEP
Sbjct: 361 LFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSLTTEFSSYSKSEMHSQHHHESFKEP 420

Query: 421 EDVRDVTLKYEST-------EETDTT-----DNVGSGLLQDKIVLPQHEGNGKTSLS 463
           EDVRDVT   ES        +  D T     +NVGSGL QDK+ LPQ EGN + S S
Sbjct: 421 EDVRDVTSNDESAGDRHRLIDGPDGTSLFGEENVGSGLPQDKVELPQREGNNEMSQS 474

BLAST of Sed0019762 vs. TAIR 10
Match: AT3G19770.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 506.5 bits (1303), Expect = 2.2e-143
Identity = 280/448 (62.50%), Postives = 337/448 (75.22%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MEN DVFLGLHDFLERMR+PSA DFVKSIKSFIV FSNN PD E+D A VQEF++KMEAA
Sbjct: 1   MENTDVFLGLHDFLERMRKPSAGDFVKSIKSFIVSFSNNAPDPEKDCAMVQEFFSKMEAA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCSEE+L+SAG+GLEKYVMTKL++RV+AS  ++V  DE+L +KM+L+QQFI 
Sbjct: 61  FRAHPLWSGCSEEELDSAGDGLEKYVMTKLFTRVFASNTEEVIADEKLFQKMSLVQQFIS 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDI+P FQNE+SWLLAQKEL KINMYKAPRDKLVC+L CCKVI+NLL+NASIASNEN
Sbjct: 121 PENLDIQPTFQNESSWLLAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
            PGADEFLPVLIYV IKANPPQLHSNLLYIQRYR +S+L GEAAYFFTNILSAESFISNI
Sbjct: 181 APGADEFLPVLIYVTIKANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSE--------VESPSDKS--DEGVARNPE---- 300
           DA ++S++E+EFE+NMESARA +SGL S+           P D+S   +  + NP+    
Sbjct: 241 DAKSISLDEAEFEKNMESARARISGLDSQTYQTGHGSAPPPRDESTLQKTQSLNPKRENT 300

Query: 301 LIETSSRASNSGTEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDH 360
           L ++ S  S SGT   +        + +E P  K +S       +S+LENKGA  LL D 
Sbjct: 301 LFQSKSSDSLSGTNELL-------NINSETPMKKAES-------ISDLENKGAT-LLKDT 360

Query: 361 TGKQAFQDYPYLFAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIH 420
              + FQ+YPY+FA  GDL   DVE LLN YK+LVFKYV L+KGL    +S + S+S + 
Sbjct: 361 EPSKVFQEYPYIFASAGDLRIGDVEGLLNSYKQLVFKYVCLTKGLG-DGTSLAPSSSPLQ 420

Query: 421 SHHNPESSKEPEDVRDVTLKYESTEETD 435
           +     +SKE ED R  +   + T+ETD
Sbjct: 421 ASSGFNTSKESEDHRRSSSDVQMTKETD 432

BLAST of Sed0019762 vs. TAIR 10
Match: AT5G09320.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 457.6 bits (1176), Expect = 1.2e-128
Identity = 250/427 (58.55%), Postives = 309/427 (72.37%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVLFSNNTPDAERDSASVQEFYAKMEAA 60
           MEN DV  GLH+FL +   PSA DF+KSIKSFIV   N  PD E+D  +VQ+F+ KME+A
Sbjct: 1   MENTDVVSGLHNFLSK---PSAKDFIKSIKSFIVSILNTAPDPEKDCDAVQDFFYKMESA 60

Query: 61  FRVHPLWSGCSEEDLESAGEGLEKYVMTKLYSRVYASLADDVKIDERLSEKMALIQQFIR 120
           FR HPLWSGCS+++L++AG+GLEKYVMTKL+ RV+AS  +DV  DE+L +K++L+QQFI 
Sbjct: 61  FRAHPLWSGCSDDELDNAGDGLEKYVMTKLFPRVFASNTEDVISDEKLFQKISLVQQFIS 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNEN 180
           PENLDI+P FQN+TSWLLAQKEL KINMY APRDKL+C+L CCKVI+NLL+NASIASN+N
Sbjct: 121 PENLDIQPTFQNQTSWLLAQKELQKINMYNAPRDKLMCILRCCKVINNLLLNASIASNQN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
            PGAD+FLPVLIYV IKANPPQ HSNLLYIQRYR QS+L GEA Y FTNILSAESFISNI
Sbjct: 181 EPGADQFLPVLIYVTIKANPPQFHSNLLYIQRYRRQSKLVGEAGYLFTNILSAESFISNI 240

Query: 241 DANALSMEESEFEENMESARALLSGLSSEVESPSDKSDEGVA--------RNPELIETSS 300
           DA +LSM+E++FE  M+SA A LSG  S+    S ++D G A        R   L+ T S
Sbjct: 241 DAKSLSMDEADFETKMKSAHARLSGPGSQ----SYQTDHGAALPTAHNTKRENMLLHTKS 300

Query: 301 RASNSGTEPSVRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHT-GKQA 360
             S SGT  ++                  ++ + +   +++LENKGAA LLND +   + 
Sbjct: 301 TDSFSGTNETL-----------------SETPIKKADPITDLENKGAATLLNDRSEATKI 360

Query: 361 FQDYPYLFAQVGDLTFNDVEELLNQYKRLVFKYVSLSKGL----SLTSSSASLSNSEIHS 415
           FQ+YPY+FA VGDL   DVE+LLN YK+LVFKYV LSKGL    SLT   + L  S++  
Sbjct: 361 FQEYPYMFASVGDLKIGDVEDLLNNYKQLVFKYVCLSKGLGDATSLTPCISPLQASKVSE 403

BLAST of Sed0019762 vs. TAIR 10
Match: AT3G19770.2 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 287.0 bits (733), Expect = 2.8e-77
Identity = 175/312 (56.09%), Postives = 215/312 (68.91%), Query Frame = 0

Query: 137 LLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLINASIASNENPPGADEFLPVLIYVII 196
           + AQKEL KINMYKAPRDKLVC+L CCKVI+NLL+NASIASNEN PGADEFLPVLIYV I
Sbjct: 1   MAAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNENAPGADEFLPVLIYVTI 60

Query: 197 KANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNIDANALSMEESEFEENM 256
           KANPPQLHSNLLYIQRYR +S+L GEAAYFFTNILSAESFISNIDA ++S++E+EFE+NM
Sbjct: 61  KANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNIDAKSISLDEAEFEKNM 120

Query: 257 ESARALLSGLSSE--------VESPSDKS--DEGVARNPE----LIETSSRASNSGTEPS 316
           ESARA +SGL S+           P D+S   +  + NP+    L ++ S  S SGT   
Sbjct: 121 ESARARISGLDSQTYQTGHGSAPPPRDESTLQKTQSLNPKRENTLFQSKSSDSLSGTNEL 180

Query: 317 VRPKSAEAKLRTEVPHAKDQSTVVEVPTLSELENKGAAMLLNDHTGKQAFQDYPYLFAQV 376
           +        + +E P  K +S       +S+LENKGA  LL D    + FQ+YPY+FA  
Sbjct: 181 L-------NINSETPMKKAES-------ISDLENKGAT-LLKDTEPSKVFQEYPYIFASA 240

Query: 377 GDLTFNDVEELLNQYKRLVFKYVSLSKGLSLTSSSASLSNSEIHSHHNPESSKEPEDVRD 435
           GDL   DVE LLN YK+LVFKYV L+KGL    +S + S+S + +     +SKE ED R 
Sbjct: 241 GDLRIGDVEGLLNSYKQLVFKYVCLTKGLG-DGTSLAPSSSPLQASSGFNTSKESEDHRR 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023525567.12.3e-21985.71vacuolar protein sorting-associated protein 9A-like [Cucurbita pepo subsp. pepo][more]
XP_022955656.18.3e-21785.29vacuolar protein sorting-associated protein 9A-like [Cucurbita moschata] >XP_022... [more]
XP_022990551.15.4e-21684.45vacuolar protein sorting-associated protein 9A-like [Cucurbita maxima] >XP_02299... [more]
KAG7032750.12.8e-21281.00Vacuolar protein sorting-associated protein 9A, partial [Cucurbita argyrosperma ... [more]
XP_022141647.19.5e-20580.51vacuolar protein sorting-associated protein 9A-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9LT313.1e-14262.50Vacuolar protein sorting-associated protein 9A OS=Arabidopsis thaliana OX=3702 G... [more]
Q10NQ39.8e-12856.92Vacuolar protein sorting-associated protein 9A OS=Oryza sativa subsp. japonica O... [more]
O743962.9e-3933.43Vacuolar protein sorting-associated protein 9a OS=Schizosaccharomyces pombe (str... [more]
O189739.7e-2732.30Rab5 GDP/GTP exchange factor OS=Bos taurus OX=9913 GN=RABGEF1 PE=1 SV=1[more]
Q9JM134.8e-2632.55Rab5 GDP/GTP exchange factor OS=Mus musculus OX=10090 GN=Rabgef1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GWY34.0e-21785.29vacuolar protein sorting-associated protein 9A-like OS=Cucurbita moschata OX=366... [more]
A0A6J1JJ312.6e-21684.45vacuolar protein sorting-associated protein 9A-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1CKF04.6e-20580.51vacuolar protein sorting-associated protein 9A-like OS=Momordica charantia OX=36... [more]
A0A1S3B4M42.2e-19980.08vacuolar protein sorting-associated protein 9A-like OS=Cucumis melo OX=3656 GN=L... [more]
A0A0A0LI505.4e-19879.04VPS9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G128630 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G19770.12.2e-14362.50Vacuolar sorting protein 9 (VPS9) domain [more]
AT5G09320.11.2e-12858.55Vacuolar sorting protein 9 (VPS9) domain [more]
AT3G19770.22.8e-7756.09Vacuolar sorting protein 9 (VPS9) domain [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003123VPS9 domainSMARTSM00167vps9_2coord: 135..255
e-value: 4.9E-30
score: 115.8
IPR003123VPS9 domainPFAMPF02204VPS9coord: 138..241
e-value: 3.9E-29
score: 100.9
IPR003123VPS9 domainPROSITEPS51205VPS9coord: 102..246
score: 42.607929
IPR041545RABX5, catalytic core helical domainPFAMPF18151DUF5601coord: 27..91
e-value: 3.0E-19
score: 69.0
NoneNo IPR availableGENE3D1.10.246.120coord: 17..97
e-value: 2.2E-28
score: 100.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 398..462
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 408..433
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..323
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 306..320
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 288..303
NoneNo IPR availablePANTHERPTHR23101:SF110BNAC09G47180D PROTEINcoord: 1..406
IPR037191VPS9 domain superfamilyGENE3D1.20.1050.80VPS9 domaincoord: 98..265
e-value: 1.5E-65
score: 222.0
IPR037191VPS9 domain superfamilySUPERFAMILY109993VPS9 domaincoord: 11..257
IPR045046Vacuolar protein sorting-associated protein 9-likePANTHERPTHR23101RAB GDP/GTP EXCHANGE FACTORcoord: 1..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0019762.1Sed0019762.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0000325 plant-type vacuole
cellular_component GO:0005802 trans-Golgi network
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity