Sed0019298 (gene) Chayote v1

BLAST of Sed0019298 vs. NCBI nr
Match: ADM47337.1 (gibberellin 3-oxidase 1, partial [Marah macrocarpa])

HSP 1 Score: 597.4 bits (1539), Expect = 7.7e-167
Identity = 294/350 (84.00%), Postives = 318/350 (90.86%), Query Frame = 0

Query: 1   MADQEIT---PPSPLVPLDFTSI-QTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDD- 60
           MADQEIT   P SPLVPLDF+S  +TVPESHIWVDSIELSPA+D  EKLSLPVIDLLDD 
Sbjct: 1   MADQEITTAPPSSPLVPLDFSSSHETVPESHIWVDSIELSPAMDLDEKLSLPVIDLLDDT 60

Query: 61  RASELIGRACEEWGMFLLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFG-GNTGYCMI 120
            ASELIG+AC++WGMF L NHGVPK  I +TE+EARRL  LP +QKMKTFG GNTGY M+
Sbjct: 61  TASELIGKACQQWGMFQLINHGVPKSIIAETEDEARRLFALPTTQKMKTFGPGNTGYGMV 120

Query: 121 PLSKYHSKSMWHEGFTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFK 180
           PLSKYHSKSMWHEGFTI GSP+DDAKKLWPSDYK+FC+VMEEYQR+MK LADRL+RLI K
Sbjct: 121 PLSKYHSKSMWHEGFTIFGSPLDDAKKLWPSDYKRFCDVMEEYQRKMKGLADRLMRLILK 180

Query: 181 FLDISDEEIMKLMFTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARN 240
           FLDIS+EEIMKLMFTPEDSSKIYTALRLNLYPPCPDPD+V+GMASHTDTSFFTI+HQARN
Sbjct: 181 FLDISEEEIMKLMFTPEDSSKIYTALRLNLYPPCPDPDRVVGMASHTDTSFFTIIHQARN 240

Query: 241 DGLQIFKDEAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYF 300
           DGLQIFKDEAGWV LSP+SG  MVNVGDLL ILSNGRFPS+LHRVMIQEKM DRLSLAYF
Sbjct: 241 DGLQIFKDEAGWVPLSPTSGTLMVNVGDLLQILSNGRFPSILHRVMIQEKMEDRLSLAYF 300

Query: 301 YTPPPDTYIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
           YTPPP  YIAPYCKPLSESPQIPLYRCVTVKEYST KS NNF+G+STVK+
Sbjct: 301 YTPPPHIYIAPYCKPLSESPQIPLYRCVTVKEYSTSKSNNNFKGLSTVKI 350

BLAST of Sed0019298 vs. NCBI nr
Match: AAF80661.1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 509.6 bits (1311), Expect = 2.1e-140
Identity = 245/349 (70.20%), Postives = 283/349 (81.09%), Query Frame = 0

Query: 1   MADQEITPPSPLVPLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASEL 60
           MADQE+ PPS LVPLDF SIQTVPESH+W +SIELSPAI   E++SLP+ID LDD ASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  IGRACEEWGMFLLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFGGN----TGYCMIPL 120
           +GRACE+WGMF L NHGVPK  I++ EEE RRL +LP  QKMKT   +    TGY M  +
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120

Query: 121 SKYHSKSMWHEGFTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFL 180
           SKYH K MWHEGFT+ GSPVDDAKKLWP+DYK+FC+VMEEYQ QMK L +R+IRLIFKFL
Sbjct: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180

Query: 181 DISDEEIMKLM-FTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARND 240
           DIS+EE+MKL+  T E + K Y ALRLN YPPCPDP +V+G+A+HTDTS FTIVHQAR+D
Sbjct: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240

Query: 241 GLQIFKDEAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFY 300
           GLQ+FKD  GWV LSP SG  +VNVGD+LHILSNGRFPS+LHRVMI E    R SLAYFY
Sbjct: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300

Query: 301 TPPPDTYIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
            PP D YI+PYCKPLSESPQIPLYRCV VKEY T K+K   +G+S +K+
Sbjct: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

BLAST of Sed0019298 vs. NCBI nr
Match: XP_022951873.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 504.6 bits (1298), Expect = 6.7e-139
Identity = 247/347 (71.18%), Postives = 282/347 (81.27%), Query Frame = 0

Query: 1   MADQEITPPSPLVPLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASEL 60
           MADQE       +PLDF S+QTVPESH+W DS ELS  ID  EK+SLP IDLLDD ASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  IGRACEEWGMFLLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFGGN---TGYCMIPLS 120
           +GRACE+WGMF LTNHG+ K  I +TEEEARRL TLP+ +KMKT       TGY    +S
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAETEEEARRLFTLPVKEKMKTLRSADRVTGYGTAVIS 120

Query: 121 KYHSKSMWHEGFTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLD 180
           KYH K MWHEGFTILGSP DDAKKLWPSD K+FC+VMEEYQ +MK L+DRLI+LIFKFLD
Sbjct: 121 KYHPKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKFLD 180

Query: 181 ISDEEIMKLMFTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGL 240
           IS EE MKL+ + +DS K YTAL+LN YPPCPDPDQV+G+A+HTDTS FTIVHQA NDGL
Sbjct: 181 ISVEETMKLLSSMDDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240

Query: 241 QIFKDEAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTP 300
           QIFKD+AGWV LSP  G+F+VNVGDLLHI+SNGRF S+LHRV IQ K V R SLAYFY P
Sbjct: 241 QIFKDKAGWVPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PPDTYIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
           PP  YI+P+CKPLSESPQ PLYRCV+VKEY T K+KNN +G+STVK+
Sbjct: 301 PPYLYISPHCKPLSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

BLAST of Sed0019298 vs. NCBI nr
Match: XP_023537854.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 499.2 bits (1284), Expect = 2.8e-137
Identity = 243/347 (70.03%), Postives = 280/347 (80.69%), Query Frame = 0

Query: 1   MADQEITPPSPLVPLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASEL 60
           MAD+E       +PLDF S+QTVPESH+W DS ELS  ID  EK+SLP IDLLDD ASEL
Sbjct: 1   MADKE------FLPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  IGRACEEWGMFLLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFGGN---TGYCMIPLS 120
           +GRACE+WGMF LTNHG+ K  I + EEEARRL TLP  +KMKT       TGY    +S
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAKMEEEARRLFTLPAKEKMKTLRSADRVTGYGTAVIS 120

Query: 121 KYHSKSMWHEGFTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLD 180
           KYHSK MWHEGFTILGSP DDAKKLWPSD K+FC+VMEEYQ +MK L+DRLI+LIFKFLD
Sbjct: 121 KYHSKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKFLD 180

Query: 181 ISDEEIMKLMFTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGL 240
           IS+EE MKL+ + +DS K YTAL+LN YPPCPDPDQV+G+A+HTDTS FTIVHQA NDGL
Sbjct: 181 ISEEETMKLLSSMDDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240

Query: 241 QIFKDEAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTP 300
           QIFKD+AGW+ LSP  G+F+VNVGDLLHI+SNGRF S+LHRV IQ K V R SLAYFY P
Sbjct: 241 QIFKDKAGWIPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PPDTYIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
           PP  YI P+CK LSESPQ PLYRCV+VKEY T K+KNN +G+ST+K+
Sbjct: 301 PPYLYITPHCKALSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTIKI 341

BLAST of Sed0019298 vs. NCBI nr
Match: XP_023002882.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 498.4 bits (1282), Expect = 4.8e-137
Identity = 243/347 (70.03%), Postives = 277/347 (79.83%), Query Frame = 0

Query: 1   MADQEITPPSPLVPLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASEL 60
           MADQE       +PLDF S+QTVPESH+W DS ELS  ID  EK+SLP IDLLDD ASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  IGRACEEWGMFLLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFGGN---TGYCMIPLS 120
           +GRACE+WGMF LTNHG+ K  I + EEEARRL TLP  +KMK        TGY    +S
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRSADRVTGYGTAVIS 120

Query: 121 KYHSKSMWHEGFTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLD 180
           KYH K MWHEGFTILGSP +DAKKLWPSD K+FC+VMEEYQ QMK L+DRLIRLIFKFLD
Sbjct: 121 KYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKFLD 180

Query: 181 ISDEEIMKLMFTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGL 240
           IS+EE MKL+ +  DS K YTAL+LN YPPCPDPDQV+G+A+HTDTS FTIVHQ  NDGL
Sbjct: 181 ISEEETMKLLSSTGDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCNDGL 240

Query: 241 QIFKDEAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTP 300
           QIFKD A WV LSP  G+F+VNVGDLLHI+SNGRFPS+LHRV IQ K V R SLAYFY P
Sbjct: 241 QIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PPDTYIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
           PP  YI+P+CKPLSESPQ PLYRCV+V+EY T K+KNN +G+ST+K+
Sbjct: 301 PPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

BLAST of Sed0019298 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.1e-70
Identity = 142/336 (42.26%), Postives = 207/336 (61.61%), Query Frame = 0

Query: 16  DFTSIQTVPESHIW--VDSIEL--SPAIDTKEKLSLPVIDLLDDRASELIGRACEEWGMF 75
           DF S+Q +PES+ W  +D   L  S  I  +   ++PVIDL D  AS+LIG AC+ WG++
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVY 80

Query: 76  LLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTF---GGNTGYCMIPLSKYHSKSMWHEG 135
            + NHG+P   +   +   + L +LP  QK K      G +GY +  +S +  K MW+EG
Sbjct: 81  QVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVSGYGIARISSFFPKLMWYEG 140

Query: 136 FTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLDISDEEIMKLMF 195
           FTI+GSP+D  ++LWP DY +FC+++ +Y   MK LA  L+ L+   L I+ E+I K   
Sbjct: 141 FTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLMCLMLDSLGITKEDI-KWAG 200

Query: 196 TPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGLQIFKDEAGWVA 255
           +     K   AL+LN YP CPDPD  +G+A HTD++F TI+ Q    GLQ+ ++ +GW+ 
Sbjct: 201 SKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWIT 260

Query: 256 LSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTPPPDTYIAPYCK 315
           + P  G  +VNVGDL HILSNG +PSVLHRV++  +   R S+AY Y PP +  I P+ K
Sbjct: 261 VPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLV-NRTRQRFSVAYLYGPPSNVEICPHAK 320

Query: 316 PLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
            +  + + PLYR VT  EY   K+K+  + +S+V+L
Sbjct: 321 LIGPT-KPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

BLAST of Sed0019298 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 255.0 bits (650), Expect = 1.2e-66
Identity = 130/333 (39.04%), Postives = 202/333 (60.66%), Query Frame = 0

Query: 14  PLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASELIGRACEEWGMFLL 73
           P DF S+   P+S+ W    +L  +    ++ +LP+IDL D   + L+G AC  WG F +
Sbjct: 21  PPDFKSL---PDSYTWTPKDDLLFSASASDE-TLPLIDLSDIHVATLVGHACTTWGAFQI 80

Query: 74  TNHGVPKFSINQTEEEARRLLTLPMSQKMK---TFGGNTGYCMIPLSKYHSKSMWHEGFT 133
           TNHGVP   ++  E     L  LP+ +K+K   +  G +GY +  ++ + +K MW EGFT
Sbjct: 81  TNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKMWSEGFT 140

Query: 134 ILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLDISDEEIMKLMFTP 193
           ++GSP+ D +KLWPS + ++CE++EEY+  M+ LA +L+      L + +++I +     
Sbjct: 141 VIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDI-QWAGPN 200

Query: 194 EDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGLQIFKDEAGWVALS 253
            D       ++LN YP CP+PD+ +G+A+HTD++  TI++Q    GLQ+F+D+ GWV   
Sbjct: 201 SDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAP 260

Query: 254 PSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTPPPDTYIAPYCKPL 313
           P  G+ +VNVGDLLHIL+NG FPSVLHR  +   +  R S+AY + PP D  I+P  K L
Sbjct: 261 PVPGSLVVNVGDLLHILTNGIFPSVLHRARVNH-VRSRFSMAYLWGPPSDIMISPLPK-L 320

Query: 314 SESPQIPLYRCVTVKEYSTRKSKNNFQGISTVK 344
            +  Q PLY  +T K+Y   K+ +  Q +S ++
Sbjct: 321 VDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of Sed0019298 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-65
Identity = 132/335 (39.40%), Postives = 203/335 (60.60%), Query Frame = 0

Query: 16  DFTSIQTVPESHIWVDSIEL----SPAIDTKEKLSLPVIDLLDDRASELIGRACEEWGMF 75
           DFTS++ +P+S+ W    +L    +P+     + ++P+IDL    A+  IG AC  WG F
Sbjct: 23  DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGE-NIPLIDLDHPDATNQIGHACRTWGAF 82

Query: 76  LLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFGGNT---GYCMIPLSKYHSKSMWHEG 135
            ++NHGVP   +   E     L  LP+ +K+K+    T   GY +  ++ + +K MW EG
Sbjct: 83  QISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASFFNKQMWSEG 142

Query: 136 FTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLDISDEEIMKLMF 195
           FTI GSP++D +KLWP  +  +C+++EEY+  MK LA +L+ L    L +S+E+I +   
Sbjct: 143 FTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSEEDI-EWAS 202

Query: 196 TPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGLQIFKDEAGWVA 255
              D +    AL+LN YP CP+PD+ +G+A+HTD++  TI++Q    GLQ+F+D+ GWV 
Sbjct: 203 LSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVT 262

Query: 256 LSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTPPPDTYIAPYCK 315
           + P  G+ +VNVGDL HILSNG F SVLHR  + +    RLS+A+ + P  D  I+P  K
Sbjct: 263 VPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQSDIKISPVPK 322

Query: 316 PLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVK 344
            L    + PLY+ VT KEY   K+ +  + +S ++
Sbjct: 323 -LVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of Sed0019298 vs. ExPASy Swiss-Prot
Match: Q9FU53 (Gibberellin 3-beta-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA3OX2 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 4.8e-63
Identity = 134/340 (39.41%), Postives = 199/340 (58.53%), Query Frame = 0

Query: 16  DFTSIQTVPESHIWVDSIELSPAID---TKEKLSLPVIDLLDDRASELIGRACEEWGMFL 75
           DF + + VPE+H W   ++  P +D      + ++PV+D+    A+  + RA E+WG FL
Sbjct: 14  DFRAARRVPETHAW-PGLDDHPVVDGGGGGGEDAVPVVDVGAGDAAARVARAAEQWGAFL 73

Query: 76  LTNHGVPKFSINQTEEEARRLLTLPMSQKMKTF---GGNTGYCMIPLSKYHSKSMWHEGF 135
           L  HGVP   +++ EE   R+ +LP S+KM+     G   GY   P+S + SK MW EG+
Sbjct: 74  LVGHGVPAALLSRVEERVARVFSLPASEKMRAVRGPGEPCGYGSPPISSFFSKLMWSEGY 133

Query: 136 TILGSPV-DDAKKLWP---SDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLDISDEEIMK 195
           T   S +  + ++LWP    DY  FC+VMEE+ ++M+ LAD L+RL  + L ++ EE+  
Sbjct: 134 TFSPSSLRSELRRLWPKSGDDYLLFCDVMEEFHKEMRRLADELLRLFLRALGLTGEEVAG 193

Query: 196 LMFTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGLQIF-KDEA 255
           +        ++   + LN YP CP+P + LG+ +HTD+ FFT V Q+   GLQ+F +   
Sbjct: 194 VEAERRIGERMTATVHLNWYPRCPEPRRALGLIAHTDSGFFTFVLQSLVPGLQLFRRGPD 253

Query: 256 GWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTPPPDTYIA 315
            WVA+   +GAF+VNVGDL HIL+NGRF SV HR ++     DR+SL YF  PPPD  +A
Sbjct: 254 RWVAVPAVAGAFVVNVGDLFHILTNGRFHSVYHRAVVNRDR-DRVSLGYFLGPPPDAEVA 313

Query: 316 PYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
           P  + +  + + P YR VT  EY   + K    G S +K+
Sbjct: 314 PLPEAV-PAGRSPAYRAVTWPEYMAVRKKAFATGGSALKM 350

BLAST of Sed0019298 vs. ExPASy Swiss-Prot
Match: Q3I409 (Gibberellin 3-beta-dioxygenase 2-3 OS=Triticum aestivum OX=4565 GN=GA3ox2-3 PE=2 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 2.6e-61
Identity = 133/343 (38.78%), Postives = 193/343 (56.27%), Query Frame = 0

Query: 16  DFTSIQTVPESHIWVDSIELSPAIDTK----EKLSLPVIDLLDDRASELIGRACEEWGMF 75
           DF + + VPE+H W   +   P +D      E  ++PV+D+ D  A+E +G A ++WG F
Sbjct: 15  DFRAARRVPETHAW-PGLHDHPVVDGSGAGGEPDAVPVVDMRDPFAAEAVGLAAQDWGAF 74

Query: 76  LLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTF---GGNTGYCMIPLSKYHSKSMWHEG 135
           LL  HGVP   + + E     +  LP S+KM+     G + GY   P+S + SK MW EG
Sbjct: 75  LLVGHGVPLDLLVRVEAAIAGMFALPASEKMRAVRRPGDSCGYGSPPISSFFSKCMWSEG 134

Query: 136 FTILGSPVD---DAKKLWPS---DYKQFCEVMEEYQRQMKVLADRLIRLIFKFLDISDEE 195
           +T   SP +   D +KLWP    DY+ FC VMEE+ R+M+ LAD+L+ L    L ++ E+
Sbjct: 135 YTF--SPANLRSDLRKLWPKAGHDYRHFCAVMEEFHREMRALADKLLELFLVALGLTGEQ 194

Query: 196 IMKLMFTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGLQIFKD 255
           +  +    + +  +   + LN YP CPDP + LG+ +HTD+ FFT V Q+   GLQ+F+ 
Sbjct: 195 VAAVESEQKIAETMTATMHLNWYPKCPDPKRALGLIAHTDSGFFTFVLQSLVPGLQLFRH 254

Query: 256 EAG-WVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTPPPDT 315
               WV +    GA +VNVGDL  IL+NGRF SV HR ++  +  DR+SL YF  PP   
Sbjct: 255 GPDRWVTVPAVPGAMVVNVGDLFQILTNGRFHSVYHRAVVNRES-DRISLGYFLGPPAHV 314

Query: 316 YIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
            +AP  + L+ +P    YR VT  EY   + K    G S +K+
Sbjct: 315 KVAPLREALAGTP--AAYRAVTWPEYMGVRKKAFTTGASALKM 351

BLAST of Sed0019298 vs. ExPASy TrEMBL
Match: E1AXG4 (Gibberellin 3-oxidase 1 (Fragment) OS=Marah macrocarpa OX=48242 PE=2 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.7e-167
Identity = 294/350 (84.00%), Postives = 318/350 (90.86%), Query Frame = 0

Query: 1   MADQEIT---PPSPLVPLDFTSI-QTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDD- 60
           MADQEIT   P SPLVPLDF+S  +TVPESHIWVDSIELSPA+D  EKLSLPVIDLLDD 
Sbjct: 1   MADQEITTAPPSSPLVPLDFSSSHETVPESHIWVDSIELSPAMDLDEKLSLPVIDLLDDT 60

Query: 61  RASELIGRACEEWGMFLLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFG-GNTGYCMI 120
            ASELIG+AC++WGMF L NHGVPK  I +TE+EARRL  LP +QKMKTFG GNTGY M+
Sbjct: 61  TASELIGKACQQWGMFQLINHGVPKSIIAETEDEARRLFALPTTQKMKTFGPGNTGYGMV 120

Query: 121 PLSKYHSKSMWHEGFTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFK 180
           PLSKYHSKSMWHEGFTI GSP+DDAKKLWPSDYK+FC+VMEEYQR+MK LADRL+RLI K
Sbjct: 121 PLSKYHSKSMWHEGFTIFGSPLDDAKKLWPSDYKRFCDVMEEYQRKMKGLADRLMRLILK 180

Query: 181 FLDISDEEIMKLMFTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARN 240
           FLDIS+EEIMKLMFTPEDSSKIYTALRLNLYPPCPDPD+V+GMASHTDTSFFTI+HQARN
Sbjct: 181 FLDISEEEIMKLMFTPEDSSKIYTALRLNLYPPCPDPDRVVGMASHTDTSFFTIIHQARN 240

Query: 241 DGLQIFKDEAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYF 300
           DGLQIFKDEAGWV LSP+SG  MVNVGDLL ILSNGRFPS+LHRVMIQEKM DRLSLAYF
Sbjct: 241 DGLQIFKDEAGWVPLSPTSGTLMVNVGDLLQILSNGRFPSILHRVMIQEKMEDRLSLAYF 300

Query: 301 YTPPPDTYIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
           YTPPP  YIAPYCKPLSESPQIPLYRCVTVKEYST KS NNF+G+STVK+
Sbjct: 301 YTPPPHIYIAPYCKPLSESPQIPLYRCVTVKEYSTSKSNNNFKGLSTVKI 350

BLAST of Sed0019298 vs. ExPASy TrEMBL
Match: Q9LM06 (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE=2 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 1.0e-140
Identity = 245/349 (70.20%), Postives = 283/349 (81.09%), Query Frame = 0

Query: 1   MADQEITPPSPLVPLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASEL 60
           MADQE+ PPS LVPLDF SIQTVPESH+W +SIELSPAI   E++SLP+ID LDD ASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  IGRACEEWGMFLLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFGGN----TGYCMIPL 120
           +GRACE+WGMF L NHGVPK  I++ EEE RRL +LP  QKMKT   +    TGY M  +
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120

Query: 121 SKYHSKSMWHEGFTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFL 180
           SKYH K MWHEGFT+ GSPVDDAKKLWP+DYK+FC+VMEEYQ QMK L +R+IRLIFKFL
Sbjct: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180

Query: 181 DISDEEIMKLM-FTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARND 240
           DIS+EE+MKL+  T E + K Y ALRLN YPPCPDP +V+G+A+HTDTS FTIVHQAR+D
Sbjct: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240

Query: 241 GLQIFKDEAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFY 300
           GLQ+FKD  GWV LSP SG  +VNVGD+LHILSNGRFPS+LHRVMI E    R SLAYFY
Sbjct: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300

Query: 301 TPPPDTYIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
            PP D YI+PYCKPLSESPQIPLYRCV VKEY T K+K   +G+S +K+
Sbjct: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

BLAST of Sed0019298 vs. ExPASy TrEMBL
Match: A0A6J1GIY0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454608 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.3e-139
Identity = 247/347 (71.18%), Postives = 282/347 (81.27%), Query Frame = 0

Query: 1   MADQEITPPSPLVPLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASEL 60
           MADQE       +PLDF S+QTVPESH+W DS ELS  ID  EK+SLP IDLLDD ASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  IGRACEEWGMFLLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFGGN---TGYCMIPLS 120
           +GRACE+WGMF LTNHG+ K  I +TEEEARRL TLP+ +KMKT       TGY    +S
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAETEEEARRLFTLPVKEKMKTLRSADRVTGYGTAVIS 120

Query: 121 KYHSKSMWHEGFTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLD 180
           KYH K MWHEGFTILGSP DDAKKLWPSD K+FC+VMEEYQ +MK L+DRLI+LIFKFLD
Sbjct: 121 KYHPKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKFLD 180

Query: 181 ISDEEIMKLMFTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGL 240
           IS EE MKL+ + +DS K YTAL+LN YPPCPDPDQV+G+A+HTDTS FTIVHQA NDGL
Sbjct: 181 ISVEETMKLLSSMDDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240

Query: 241 QIFKDEAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTP 300
           QIFKD+AGWV LSP  G+F+VNVGDLLHI+SNGRF S+LHRV IQ K V R SLAYFY P
Sbjct: 241 QIFKDKAGWVPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PPDTYIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
           PP  YI+P+CKPLSESPQ PLYRCV+VKEY T K+KNN +G+STVK+
Sbjct: 301 PPYLYISPHCKPLSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

BLAST of Sed0019298 vs. ExPASy TrEMBL
Match: A0A6J1KKU0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111496619 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 2.3e-137
Identity = 243/347 (70.03%), Postives = 277/347 (79.83%), Query Frame = 0

Query: 1   MADQEITPPSPLVPLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASEL 60
           MADQE       +PLDF S+QTVPESH+W DS ELS  ID  EK+SLP IDLLDD ASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  IGRACEEWGMFLLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFGGN---TGYCMIPLS 120
           +GRACE+WGMF LTNHG+ K  I + EEEARRL TLP  +KMK        TGY    +S
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRSADRVTGYGTAVIS 120

Query: 121 KYHSKSMWHEGFTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLD 180
           KYH K MWHEGFTILGSP +DAKKLWPSD K+FC+VMEEYQ QMK L+DRLIRLIFKFLD
Sbjct: 121 KYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKFLD 180

Query: 181 ISDEEIMKLMFTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGL 240
           IS+EE MKL+ +  DS K YTAL+LN YPPCPDPDQV+G+A+HTDTS FTIVHQ  NDGL
Sbjct: 181 ISEEETMKLLSSTGDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCNDGL 240

Query: 241 QIFKDEAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTP 300
           QIFKD A WV LSP  G+F+VNVGDLLHI+SNGRFPS+LHRV IQ K V R SLAYFY P
Sbjct: 241 QIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PPDTYIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
           PP  YI+P+CKPLSESPQ PLYRCV+V+EY T K+KNN +G+ST+K+
Sbjct: 301 PPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

BLAST of Sed0019298 vs. ExPASy TrEMBL
Match: S0BE47 (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.8e-130
Identity = 228/350 (65.14%), Postives = 275/350 (78.57%), Query Frame = 0

Query: 1   MADQEITPPSPLVPLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASEL 60
           MADQEI PPS LVPLDFTS+QTVPESH+W ++ ELS AI   +++SLP+IDL+ D ASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  IGRACEEWGMFLLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFGG-----NTGYCMIP 120
           +GRACEEWGMF L NHG+ K  I + EEE RRL TLP +QKMKT         TGY M  
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 LSKYHSKSMWHEGFTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKF 180
           +SKYH K MWHEGFTILGSP+++AKKLWP+DYK+FC+VMEEYQR+MK L +R+IRLI KF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LDISDEEIMKLMFTPEDS-SKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARN 240
           L IS+EE+MKL+ + ++S  K + ALRLN YPPCPDP QV+G+A+HTDTS  TI+HQ  N
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 DGLQIFKDEAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYF 300
           +GLQIFKD  GWV LSP SG  +VN+GDLLHILSNGRFPS+LHRVMIQE    R SLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YTPPPDTYIAPYCKPLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVKL 345
           Y PP + YI+PYCKPLS++PQ PLYR V VKEY   K+K   +G+  +K+
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

BLAST of Sed0019298 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 255.0 bits (650), Expect = 8.7e-68
Identity = 130/333 (39.04%), Postives = 202/333 (60.66%), Query Frame = 0

Query: 14  PLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASELIGRACEEWGMFLL 73
           P DF S+   P+S+ W    +L  +    ++ +LP+IDL D   + L+G AC  WG F +
Sbjct: 21  PPDFKSL---PDSYTWTPKDDLLFSASASDE-TLPLIDLSDIHVATLVGHACTTWGAFQI 80

Query: 74  TNHGVPKFSINQTEEEARRLLTLPMSQKMK---TFGGNTGYCMIPLSKYHSKSMWHEGFT 133
           TNHGVP   ++  E     L  LP+ +K+K   +  G +GY +  ++ + +K MW EGFT
Sbjct: 81  TNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKMWSEGFT 140

Query: 134 ILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLDISDEEIMKLMFTP 193
           ++GSP+ D +KLWPS + ++CE++EEY+  M+ LA +L+      L + +++I +     
Sbjct: 141 VIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDI-QWAGPN 200

Query: 194 EDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGLQIFKDEAGWVALS 253
            D       ++LN YP CP+PD+ +G+A+HTD++  TI++Q    GLQ+F+D+ GWV   
Sbjct: 201 SDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAP 260

Query: 254 PSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTPPPDTYIAPYCKPL 313
           P  G+ +VNVGDLLHIL+NG FPSVLHR  +   +  R S+AY + PP D  I+P  K L
Sbjct: 261 PVPGSLVVNVGDLLHILTNGIFPSVLHRARVNH-VRSRFSMAYLWGPPSDIMISPLPK-L 320

Query: 314 SESPQIPLYRCVTVKEYSTRKSKNNFQGISTVK 344
            +  Q PLY  +T K+Y   K+ +  Q +S ++
Sbjct: 321 VDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of Sed0019298 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 251.9 bits (642), Expect = 7.3e-67
Identity = 132/335 (39.40%), Postives = 203/335 (60.60%), Query Frame = 0

Query: 16  DFTSIQTVPESHIWVDSIEL----SPAIDTKEKLSLPVIDLLDDRASELIGRACEEWGMF 75
           DFTS++ +P+S+ W    +L    +P+     + ++P+IDL    A+  IG AC  WG F
Sbjct: 23  DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGE-NIPLIDLDHPDATNQIGHACRTWGAF 82

Query: 76  LLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFGGNT---GYCMIPLSKYHSKSMWHEG 135
            ++NHGVP   +   E     L  LP+ +K+K+    T   GY +  ++ + +K MW EG
Sbjct: 83  QISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASFFNKQMWSEG 142

Query: 136 FTILGSPVDDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLDISDEEIMKLMF 195
           FTI GSP++D +KLWP  +  +C+++EEY+  MK LA +L+ L    L +S+E+I +   
Sbjct: 143 FTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSEEDI-EWAS 202

Query: 196 TPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGLQIFKDEAGWVA 255
              D +    AL+LN YP CP+PD+ +G+A+HTD++  TI++Q    GLQ+F+D+ GWV 
Sbjct: 203 LSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVT 262

Query: 256 LSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTPPPDTYIAPYCK 315
           + P  G+ +VNVGDL HILSNG F SVLHR  + +    RLS+A+ + P  D  I+P  K
Sbjct: 263 VPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQSDIKISPVPK 322

Query: 316 PLSESPQIPLYRCVTVKEYSTRKSKNNFQGISTVK 344
            L    + PLY+ VT KEY   K+ +  + +S ++
Sbjct: 323 -LVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of Sed0019298 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 226.9 bits (577), Expect = 2.5e-59
Identity = 123/330 (37.27%), Postives = 198/330 (60.00%), Query Frame = 0

Query: 12  LVPLDFTSIQTVPESHIWVDSIELSPAIDTKEKLSLPVIDLLDDRASELIGRACEEWGMF 71
           ++PLDFT+ +T+P+SH+W       P  +T     +PVI L +     L+ +ACEEWG+F
Sbjct: 18  IIPLDFTNTKTLPDSHVW-----SKPEPETTSG-PIPVISLSNPEEHGLLRQACEEWGVF 77

Query: 72  LLTNHGVPKFSINQTEEEARRLLTLPMSQK---MKTFGGNTGYCMIPLSKYHSKSMWHEG 131
            +T+HGV    ++  + + +RL +LPM +K   +++   +TGY ++ +S ++ K MW EG
Sbjct: 78  HITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWSEG 137

Query: 132 FTILGSPV-DDAKKLWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLDISDEEIMKLM 191
           F+++GS +   A  LWP D+ +FC VMEEYQ+ M  L+ RLI ++   L ++ E++  L+
Sbjct: 138 FSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLGWLV 197

Query: 192 --FTPEDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGLQI---FKD 251
              T   +  I + L+LN YP CPDP   +G+A HTD+S  TI++Q    GL+I    ++
Sbjct: 198 PDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEE 257

Query: 252 EAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTPPPDTY 311
            + W+ + P  G+ +V +GDL HI+SNG+F S +HR ++  K   R+S AYF  PP +  
Sbjct: 258 GSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVV-NKTHHRVSAAYFAGPPKNLQ 317

Query: 312 IAPYCKPLSESPQIPLYRCVTVKEYSTRKS 333
           I P     S+    P+YR +  +EY   K+
Sbjct: 318 IGPL---TSDKNHPPIYRRLIWEEYLAAKA 337

BLAST of Sed0019298 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 224.2 bits (570), Expect = 1.6e-58
Identity = 125/326 (38.34%), Postives = 193/326 (59.20%), Query Frame = 0

Query: 19  SIQTVPESHIW----VDSI---ELSPAIDTKEKLSLPVIDLLDDRASELIGRACEEWGMF 78
           S+QT+PES  W     DS+     + + D  E+ S+PVIDL +   + LIG A + WG F
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEE-SIPVIDLSNPDVTTLIGDASKTWGAF 74

Query: 79  LLTNHGVPKFSINQTEEEARRLLTLPMSQKMKTFG---GNTGYCMIPLSKYHSKSMWHEG 138
            + NHG+ +  ++  E  ++ L  +P  +K++      G +GY    +S +  K MW EG
Sbjct: 75  QIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEG 134

Query: 139 FTILGSPVDDAKK-----LWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLDISDEEI 198
           FTI     DD+ +     LWP D+ ++C +++EY  +M+ LA RL+  I   L ++ E+I
Sbjct: 135 FTI----ADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDI 194

Query: 199 MKLMFTPEDSSKI-YTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARNDGLQIFKD 258
                  +  SK+   A+RLN YP CP+P++ +G+A+HTD++  TI+HQ+   GLQ+F++
Sbjct: 195 EWAHKLEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFRE 254

Query: 259 EAGWVALSPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFY-TPPPDT 318
           E+GWV + P+ G  +VN+GDL HILSNG+ PSV+HR  +      R+S+AY +  P  D 
Sbjct: 255 ESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDV 314

Query: 319 YIAPYCKPLSESPQIPLYRCVTVKEY 328
            IAP  K L+   +  LYR +T KEY
Sbjct: 315 QIAPISK-LTGPAEPSLYRSITWKEY 333

BLAST of Sed0019298 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 5.3e-41
Identity = 98/318 (30.82%), Postives = 165/318 (51.89%), Query Frame = 0

Query: 22  TVPESHIWVDSIELSPAIDTKEKLSLPVIDLLD-------DRASELIGRACEEWGMFLLT 81
           T+P  ++ VD  +     D+     +PVID+         D   + +  AC++WG F L 
Sbjct: 28  TIPPRYVRVDQEKTEILNDSSLSSEIPVIDMTRLCSVSAMDSELKKLDFACQDWGFFQLV 87

Query: 82  NHGVPKFSINQTEEEARRLLTLPMSQKMKTF---GGNTGYCMIPLSKYHSKSMWHEGFTI 141
           NHG+    + + E E +    LPM +K K +   G   G+  + +   + K  W + F +
Sbjct: 88  NHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEGFGQVNIVSENQKLDWGDMFIL 147

Query: 142 LGSPVDDAKK-LWPSDYKQFCEVMEEYQRQMKVLADRLIRLIFKFLDISDEEIMKLMFTP 201
              P+   K  L+      F E +E Y  ++K +A  L   +   L+I  EE+  L    
Sbjct: 148 TTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILFAKMASVLEIKHEEMEDLF--- 207

Query: 202 EDSSKIYTALRLNLYPPCPDPDQVLGMASHTDTSFFTIVHQARN-DGLQIFKDEAGWVAL 261
                ++ ++++N YPPCP PDQV+G+  H+D +  TI+ Q    +GLQI KD   WV +
Sbjct: 208 ---DDVWQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQVEGLQIKKD-GKWVVV 267

Query: 262 SPSSGAFMVNVGDLLHILSNGRFPSVLHRVMIQEKMVDRLSLAYFYTPPPDTYIAPYCKP 321
            P   A +VNVG++L I++NGR+ S+ HR ++  +  +RLS+A F++P  +T I P  K 
Sbjct: 268 KPLRDALVVNVGEILEIITNGRYRSIEHRAVVNSEK-ERLSVAMFHSPGKETIIRP-AKS 327

Query: 322 LSESPQIPLYRCVTVKEY 328
           L +  +  L++ ++ +EY
Sbjct: 328 LVDRQKQCLFKSMSTQEY 336