Sed0018928 (gene) Chayote v1

Overview
NameSed0018928
Typegene
OrganismSechium edule (Chayote v1)
Descriptionindole-3-acetic acid-amido synthetase GH3.6-like
LocationLG01: 5075180 .. 5077365 (+)
RNA-Seq ExpressionSed0018928
SyntenySed0018928
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAGCACTAAGTTCTCCCAAACATTTCTGAGGGTTGCACCTTCATTTTTCAAAACTTCAAAACTTCAAATCCAAAAAAATGTCGTCTTACGAAGAAAACCTGTTGGATGAGAAAGACAAGCAAGCCCTGCAACATATAGAGGATGTCACGATCAACGCCGACAAAATCCAACAACAAACTCTAATCGAAATTCTGTCAACAAATGGGCATGTCGAGTACTTGCAGCAACATGGCCTTAACGGATCTACTGATGTTTCAACTTTCAAAAATCTTATACCTCTCGTATCTTATGAACAACTGAAACCTTATATTGATCGTATTGCAAATGGTGATGATTCTCCAATCTTATGCGCACAACCCATTACTGAATTCTTTATCAGGTGACTCTTTTCTCTGAAACACAATCTTTAAACTTCTTATTCTATTTAGATGTAGGATTTAATTAATTATAAGGACATTGCACTTTTGTTGTAGCTCTGGAACATCGGGGGGTGAACAAAAGTTAATTCCAATGTTCGACCAACAGTTTGAGAGAATGTTGACTTTTTTTAACTATGTGATCCCAATAATGAAACAGTTGTTTCCAAATTCCCACAAAGGAAAAGAAATGTTCTTTTGTTTCACCAGGGCTGCGTCTACAACTAAAGCTGGAATTATTGCTCATCCTACATCGACAAGATTGTGCAAGACATCAGTCTTCAACAATATTCAAAGTGGTGGTAACACGAGTCCTAACGACGTCCTTTTATGCACAGACACGTACCAAAGCATATATTGTCAATTGCTTTGTGGGCTCTGCCAAAACGAGACGGTTCTTAAATTAGGTGCTCTTTTTGTCTCAACTATAGTTTATGTTCTTAAGTTCCTAAGCAATCATTGGATTCTCTTGGCTAATGATATTCGAACAGGAACCATAAATTCGAAAATTATAGATTCATCGGTGAGAGAATCAGTTATGAACATCCTTAAACCGAATCCACAACTTGCAGATTTCATTGAAACAGAATGTGGCCAAAGATCATGGGAAGGAATCGTTACAAGATTGTGGCCAAACATTGAGTACATTAAAGCTATTGCTTCTGGAAGCATGTCACAATACATTCCCCTGATTAATTACTATAGTAATAACCTCCCTCTTATTTCAGATCACTATGGTTCTTCAGAGTGCTTCTTTGGCTTGAACTTAGATCCATTATGCAAACCAGAGGAAATAAGTTACACTTTAATCCCCAACGTGGCATATTTTGAGTTCTTACCAGTTGAAAGGGAAAAAAGTAATGGGGAAACAAGGCAAGAATTAGTTGATCTTGTGGATGTAAAGTTAGGACAAGAATATGAGCTAGTCATCACCACTTATGTTGGCCTCTACCGTTACTGTGTTGGTGACATCCTTCGCGTCATAGGGTTTAAAAACAAAGCTCCAAACTTTAGTTTTGTGTGTAGGAAAAACATAATTCTAAGCTTGAATGTTGAGAAAACAGAAGAGGATGAACTTCATAATGCTATACAACAGGCTGCGCGTCTTTTAACCCCATTTGGAGCTGTTGTTGTTGACTACACAAGTTATGGCAATAACACAACAATTCCTGGACATTATGTGTTGTATTGGGAAATTCGTGTCGAGAAAGAAAGCTCTATGATTCCATCTTCCATCTATGAGGAGTGTTACCTTGTTGTTGAAGAGTTGTTGAATGTTCAGTATCGTCAAGGAAGATCTCAAGACAACCTTTTAAACCCATTAGAGATTAGAATTGTAGAGTGTGGAACTTTTGATATTTTGATGGAGCGAGCAATGAATCAAGGGGTGTCAATTAGTCAATACAAGACACCTCGAAGCATAAAATGTACACAGATTATCGAGCTTTTGGATGCAAGAGTTTTGTCCAACCATTTTAGCCAAAAATCTCCAAAATGGGATCCCACTAGAAGACACCATCTTTTTTCCTCCTAGTTTATGCAAAAAAAAAAAAAAAAGAAGTTAAGAGAAAAATAAAAATCACATTAAATAATGGTAGTTTTAGTTTTTGTGTATGTCCTTTTGGGTTTTTTTTACTTACTTTCTGTTGCTTTACTGAAAGCTTGTTTGCAAGCACGGTTTGCGCTCCAAGAGTTGTAGGCTCTCATTTGGATTGTAGTCCCTAATTCTAAGTGCTCTTTGCTGCAAAATAGAAAAAGAATATTTTCG

mRNA sequence

TTCAGCACTAAGTTCTCCCAAACATTTCTGAGGGTTGCACCTTCATTTTTCAAAACTTCAAAACTTCAAATCCAAAAAAATGTCGTCTTACGAAGAAAACCTGTTGGATGAGAAAGACAAGCAAGCCCTGCAACATATAGAGGATGTCACGATCAACGCCGACAAAATCCAACAACAAACTCTAATCGAAATTCTGTCAACAAATGGGCATGTCGAGTACTTGCAGCAACATGGCCTTAACGGATCTACTGATGTTTCAACTTTCAAAAATCTTATACCTCTCGTATCTTATGAACAACTGAAACCTTATATTGATCGTATTGCAAATGGTGATGATTCTCCAATCTTATGCGCACAACCCATTACTGAATTCTTTATCAGCTCTGGAACATCGGGGGGTGAACAAAAGTTAATTCCAATGTTCGACCAACAGTTTGAGAGAATGTTGACTTTTTTTAACTATGTGATCCCAATAATGAAACAGTTGTTTCCAAATTCCCACAAAGGAAAAGAAATGTTCTTTTGTTTCACCAGGGCTGCGTCTACAACTAAAGCTGGAATTATTGCTCATCCTACATCGACAAGATTGTGCAAGACATCAGTCTTCAACAATATTCAAAGTGGTGGTAACACGAGTCCTAACGACGTCCTTTTATGCACAGACACGTACCAAAGCATATATTGTCAATTGCTTTGTGGGCTCTGCCAAAACGAGACGGTTCTTAAATTAGGTGCTCTTTTTGTCTCAACTATAGTTTATGTTCTTAAGTTCCTAAGCAATCATTGGATTCTCTTGGCTAATGATATTCGAACAGGAACCATAAATTCGAAAATTATAGATTCATCGGTGAGAGAATCAGTTATGAACATCCTTAAACCGAATCCACAACTTGCAGATTTCATTGAAACAGAATGTGGCCAAAGATCATGGGAAGGAATCGTTACAAGATTGTGGCCAAACATTGAGTACATTAAAGCTATTGCTTCTGGAAGCATGTCACAATACATTCCCCTGATTAATTACTATAGTAATAACCTCCCTCTTATTTCAGATCACTATGGTTCTTCAGAGTGCTTCTTTGGCTTGAACTTAGATCCATTATGCAAACCAGAGGAAATAAGTTACACTTTAATCCCCAACGTGGCATATTTTGAGTTCTTACCAGTTGAAAGGGAAAAAAGTAATGGGGAAACAAGGCAAGAATTAGTTGATCTTGTGGATGTAAAGTTAGGACAAGAATATGAGCTAGTCATCACCACTTATGTTGGCCTCTACCGTTACTGTGTTGGTGACATCCTTCGCGTCATAGGGTTTAAAAACAAAGCTCCAAACTTTAGTTTTGTGTGTAGGAAAAACATAATTCTAAGCTTGAATGTTGAGAAAACAGAAGAGGATGAACTTCATAATGCTATACAACAGGCTGCGCGTCTTTTAACCCCATTTGGAGCTGTTGTTGTTGACTACACAAGTTATGGCAATAACACAACAATTCCTGGACATTATGTGTTGTATTGGGAAATTCGTGTCGAGAAAGAAAGCTCTATGATTCCATCTTCCATCTATGAGGAGTGTTACCTTGTTGTTGAAGAGTTGTTGAATGTTCAGTATCGTCAAGGAAGATCTCAAGACAACCTTTTAAACCCATTAGAGATTAGAATTGTAGAGTGTGGAACTTTTGATATTTTGATGGAGCGAGCAATGAATCAAGGGGTGTCAATTAGTCAATACAAGACACCTCGAAGCATAAAATGTACACAGATTATCGAGCTTTTGGATGCAAGAGTTTTGTCCAACCATTTTAGCCAAAAATCTCCAAAATGGGATCCCACTAGAAGACACCATCTTTTTTCCTCCTAGTTTATGCAAAAAAAAAAAAAAAAGAAGTTAAGAGAAAAATAAAAATCACATTAAATAATGGTAGTTTTAGTTTTTGTGTATGTCCTTTTGGGTTTTTTTTACTTACTTTCTGTTGCTTTACTGAAAGCTTGTTTGCAAGCACGGTTTGCGCTCCAAGAGTTGTAGGCTCTCATTTGGATTGTAGTCCCTAATTCTAAGTGCTCTTTGCTGCAAAATAGAAAAAGAATATTTTCG

Coding sequence (CDS)

ATGTCGTCTTACGAAGAAAACCTGTTGGATGAGAAAGACAAGCAAGCCCTGCAACATATAGAGGATGTCACGATCAACGCCGACAAAATCCAACAACAAACTCTAATCGAAATTCTGTCAACAAATGGGCATGTCGAGTACTTGCAGCAACATGGCCTTAACGGATCTACTGATGTTTCAACTTTCAAAAATCTTATACCTCTCGTATCTTATGAACAACTGAAACCTTATATTGATCGTATTGCAAATGGTGATGATTCTCCAATCTTATGCGCACAACCCATTACTGAATTCTTTATCAGCTCTGGAACATCGGGGGGTGAACAAAAGTTAATTCCAATGTTCGACCAACAGTTTGAGAGAATGTTGACTTTTTTTAACTATGTGATCCCAATAATGAAACAGTTGTTTCCAAATTCCCACAAAGGAAAAGAAATGTTCTTTTGTTTCACCAGGGCTGCGTCTACAACTAAAGCTGGAATTATTGCTCATCCTACATCGACAAGATTGTGCAAGACATCAGTCTTCAACAATATTCAAAGTGGTGGTAACACGAGTCCTAACGACGTCCTTTTATGCACAGACACGTACCAAAGCATATATTGTCAATTGCTTTGTGGGCTCTGCCAAAACGAGACGGTTCTTAAATTAGGTGCTCTTTTTGTCTCAACTATAGTTTATGTTCTTAAGTTCCTAAGCAATCATTGGATTCTCTTGGCTAATGATATTCGAACAGGAACCATAAATTCGAAAATTATAGATTCATCGGTGAGAGAATCAGTTATGAACATCCTTAAACCGAATCCACAACTTGCAGATTTCATTGAAACAGAATGTGGCCAAAGATCATGGGAAGGAATCGTTACAAGATTGTGGCCAAACATTGAGTACATTAAAGCTATTGCTTCTGGAAGCATGTCACAATACATTCCCCTGATTAATTACTATAGTAATAACCTCCCTCTTATTTCAGATCACTATGGTTCTTCAGAGTGCTTCTTTGGCTTGAACTTAGATCCATTATGCAAACCAGAGGAAATAAGTTACACTTTAATCCCCAACGTGGCATATTTTGAGTTCTTACCAGTTGAAAGGGAAAAAAGTAATGGGGAAACAAGGCAAGAATTAGTTGATCTTGTGGATGTAAAGTTAGGACAAGAATATGAGCTAGTCATCACCACTTATGTTGGCCTCTACCGTTACTGTGTTGGTGACATCCTTCGCGTCATAGGGTTTAAAAACAAAGCTCCAAACTTTAGTTTTGTGTGTAGGAAAAACATAATTCTAAGCTTGAATGTTGAGAAAACAGAAGAGGATGAACTTCATAATGCTATACAACAGGCTGCGCGTCTTTTAACCCCATTTGGAGCTGTTGTTGTTGACTACACAAGTTATGGCAATAACACAACAATTCCTGGACATTATGTGTTGTATTGGGAAATTCGTGTCGAGAAAGAAAGCTCTATGATTCCATCTTCCATCTATGAGGAGTGTTACCTTGTTGTTGAAGAGTTGTTGAATGTTCAGTATCGTCAAGGAAGATCTCAAGACAACCTTTTAAACCCATTAGAGATTAGAATTGTAGAGTGTGGAACTTTTGATATTTTGATGGAGCGAGCAATGAATCAAGGGGTGTCAATTAGTCAATACAAGACACCTCGAAGCATAAAATGTACACAGATTATCGAGCTTTTGGATGCAAGAGTTTTGTCCAACCATTTTAGCCAAAAATCTCCAAAATGGGATCCCACTAGAAGACACCATCTTTTTTCCTCCTAG

Protein sequence

MSSYEENLLDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPLVSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNYVIPIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQSGGNTSPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRTGTINSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGSMSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVEREKSNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRVEKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQGVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRRHHLFSS
Homology
BLAST of Sed0018928 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 845.5 bits (2183), Expect = 2.8e-241
Identity = 399/580 (68.79%), Postives = 478/580 (82.41%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPL 68
           LDEKDKQALQ +EDVT  AD+IQ++ L EILSTN HVEYLQQHGLNGSTD STFKNLIP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  VSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNY 128
           VSYE LKPYIDRI NGDDSPILCA PITEF+ S+GTSGGEQKLIP  +Q+F R L+  NY
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 VIPIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQ-SGGNTSP 188
           + P MKQLFP  H GKEMFF F +  S TKAGI+A   ST   K+   NN++     TSP
Sbjct: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183

Query: 189 NDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRTGT 248
            D+L CTD YQS+YCQLLCGLCQN+ V+++G+LF ST++Y+ KFL NHW+LLANDIRTGT
Sbjct: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243

Query: 249 INSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGSMS 308
           IN+KI+DS VR+SVMNILKP+PQLADFIE+EC + +WEGIVTRLWPN ++I A+ +GSMS
Sbjct: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303

Query: 309 QYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVEREK 368
            YIP +NYY  +LPLI+  Y SSECFFG+NLDPLCKPEEISYT IP VAYFEFLPV+R  
Sbjct: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363

Query: 369 SNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVCRKNI 428
            +GE  QE VDLVDVKLGQEYE+V+TTY GLYRYC+GDI+RV GFKNKAPNF FV RKN+
Sbjct: 364 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 423

Query: 429 ILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRVEKES 488
           ILSL+VEKT+E ELHNA+ +   LL+ FGA++VDYTSY +++T PGHYVLYWEI+ ++E 
Sbjct: 424 ILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEI 483

Query: 489 SMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQGVSI 548
           S+IPSSIYE+C L +EE L+  YR+ RS   ++NPLEI+IVE  TFD LMER ++QG SI
Sbjct: 484 SIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 543

Query: 549 SQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRRH 588
           SQYKTPR +K TQIIE LD++V++N+FS+K PKWDPTR H
Sbjct: 544 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583

BLAST of Sed0018928 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 824.3 bits (2128), Expect = 6.6e-235
Identity = 393/593 (66.27%), Postives = 484/593 (81.62%), Query Frame = 0

Query: 1   MSSYEENLLDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVS 60
           M+  +E   DE+DKQALQHIEDVTI+A +IQ+Q L +ILSTN  VEYLQQHGL+GST++S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLIPLVSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFE 120
           TFKNLIPL+SYEQL+PYIDRIANGDDSPILC  PITEFFISSGTSGGE KLIPM++++F 
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RMLTFFNYVIPIMKQLFPNS--HKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNN 180
           R L+FF+YV+P MKQLFPNS   KGK M F F +    T++GI+AH  +T LCK +  N 
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN- 180

Query: 181 IQSGGNTSPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWIL 240
                NTSP D++LCTDT QS+YCQLLCGLCQN+ V ++GALF ST+++VLKFL +HW++
Sbjct: 181 -----NTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTINSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYI 300
           LA+DIR GT+N+KI D SVRE+VMNIL PN QLADFIE EC + +WEGI+TRLWPN +YI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 KAIASGSMSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYF 360
            AI +GSM+QY+P +NYYSN+LP++SDHYG SECF GLNLDPLC P+  SYTLIP +AYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPVEREKSNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPN 420
           EFLP++R KS+ E  +ELVDLVDVKLG EYELVITTY GLYRYC+GDILRV GFKNKAPN
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYCMGDILRVTGFKNKAPN 420

Query: 421 FSFVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLY 480
           FSFVCRKN++LSL++EKT+E ELH+A+Q+AARLL+PFGA + DYTS+ +++ IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 WEIRVEKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILME 540
           WEI V K    IPSSIYE+C   +EELLN  YR  RS +  +NPLEI+IV+ GTF+ LM 
Sbjct: 481 WEIHV-KGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 541 RAMNQGVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRRHHLFS 592
           +A+N+G S+SQYKTPR +  T IIELLD+ V++ +FS++ PK D    H L S
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKVDTNALHRLLS 586

BLAST of Sed0018928 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 817.4 bits (2110), Expect = 8.1e-233
Identity = 392/593 (66.10%), Postives = 482/593 (81.28%), Query Frame = 0

Query: 1   MSSYEENLLDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVS 60
           M+  +E   DE+DKQALQHIEDVTI+A +IQ+Q L +ILSTN  VEYLQQHGL+GST++S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLIPLVSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFE 120
           TFKNLIPL+SYEQL+PYIDRIANGDDSPILC  PITEFFISSGTSGGE KLIPM++++F 
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RMLTFFNYVIPIMKQLFPNS--HKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNN 180
           R L+FF+YV+P MKQLFPNS   KGK M F F +    T++GI+AH  +T LCK +  N 
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN- 180

Query: 181 IQSGGNTSPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWIL 240
                NTSP D++LCTDT QS+YCQLLCGLCQN+ V ++GALF ST+++VLKFL +HW++
Sbjct: 181 -----NTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTINSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYI 300
           LA+DIR GT+N+KI D SVRE+VMNIL PN QLADFIE EC + +WEGI+TRLWPN +YI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 KAIASGSMSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYF 360
            AI +GSM+QY+P +NYYSN+LP++SDHYG+SECF GLNLDPLC P+  SYTLIP +AYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPVEREKSNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPN 420
           EFLP++R KS+ E  +ELVDLVDVKLG EYELVITTY GLYRY +GDIL V GFKNKAPN
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPN 420

Query: 421 FSFVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLY 480
           FSFVCRKN++LSL++EKT+E ELH+A+Q+AARLL+PFGA + DYTS+ +++ IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 WEIRVEKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILME 540
           WEI V K    IPSSIYE+C   +EELLN  YR  RS +  +NPLEI+IV+ GTF+ LM 
Sbjct: 481 WEIHV-KGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 541 RAMNQGVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRRHHLFS 592
           +A+N+G S+SQYKTPR +  T IIELLD+ V+  +FS+K PK D    H L S
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

BLAST of Sed0018928 vs. NCBI nr
Match: XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])

HSP 1 Score: 793.5 bits (2048), Expect = 1.3e-225
Identity = 378/577 (65.51%), Postives = 473/577 (81.98%), Query Frame = 0

Query: 6   ENLLDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNL 65
           +++LD KDKQ L HIED T NAD+IQ++ + EILS N HVEYL+Q GL+GSTD+STFK L
Sbjct: 3   DDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKKL 62

Query: 66  IPLVSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTF 125
           IP+VSYEQLKPYIDRIA+GDDSPILCA  IT+F IS+GTSGG QKLIPMF  +  R L  
Sbjct: 63  IPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLAT 122

Query: 126 FNYVIPIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQ-SGGN 185
             Y IP+MKQLFP    GKEMFF FTRA + TK G++A   S+ L K S+F++++     
Sbjct: 123 NYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRAY 182

Query: 186 TSPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIR 245
           TSP D++ CTD YQS+YCQLLCGLCQN+ VL++GALF ST++Y+ KFL NHW LL NDIR
Sbjct: 183 TSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDIR 242

Query: 246 TGTINSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASG 305
           TGT+++KI D  VRESVM++LKPNP+LADFIE+EC + +WEGIVTRLWPN +YI  + +G
Sbjct: 243 TGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVTG 302

Query: 306 SMSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVE 365
           SMSQYIP +N+Y    P++S  Y SSECFFG+NLDPLCKPEEISYT +P +AYFEFLPV+
Sbjct: 303 SMSQYIPQLNFYC-KCPIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPVD 362

Query: 366 REKS-NGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVC 425
           R  S NGE  QELVDLVDVKLGQEYE+V+TTY GLYRYC+GDILRV GFKNK PNFSF C
Sbjct: 363 RANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFAC 422

Query: 426 RKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRV 485
           RKN+ILSL++EKT+E +LH+A+ +A+ LL+P GA++VDYTSY +++T PGHYVLYWEI+ 
Sbjct: 423 RKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQC 482

Query: 486 EKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQ 545
            +E+S IPS++YE+C L VEE L+  YRQ RS + ++NPLEI+IVE GTF+ LME+A+++
Sbjct: 483 REETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVDR 542

Query: 546 GVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPK 581
           G SISQYKTPR +K TQ+IELLD++V+SN+FSQ+ PK
Sbjct: 543 GASISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

BLAST of Sed0018928 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 772.7 bits (1994), Expect = 2.3e-219
Identity = 380/598 (63.55%), Postives = 473/598 (79.10%), Query Frame = 0

Query: 1   MSSYEENLLDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVS 60
           MS   E+ LDEKDK+ALQHIEDVT  AD+IQ+Q L EILSTN +VEYLQQHGL+GSTD S
Sbjct: 1   MSHVVEHWLDEKDKEALQHIEDVTSKADEIQRQILTEILSTNANVEYLQQHGLHGSTDAS 60

Query: 61  TFKNLIPLVSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFE 120
           TFK LIPL+SYEQL PY+  IA+ DDSPILC+ PIT FF SSGTSGGE KLIPM++++F 
Sbjct: 61  TFKKLIPLISYEQLTPYLALIAHDDDSPILCSNPITHFFTSSGTSGGETKLIPMYEEEFV 120

Query: 121 RMLTFFNYVIPIMKQLFPNSH--KGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNN 180
           R L+FFNY++P MKQLFPN +  KGK M F F +    TKAGII     T L K S  +N
Sbjct: 121 RRLSFFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTKAGIIVRSIFTSLHKRS--SN 180

Query: 181 IQS---GGNTSPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNH 240
           ++S   G NTSP+D++LCTD++QS+YCQLLCGL QN+ V +LGALF ST+++V KFL NH
Sbjct: 181 LKSKPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRLGALFASTLIHVFKFLENH 240

Query: 241 WILLANDIRTGTINSKIIDSSVRESVMNI-LKPNPQLADFIETECGQRSWEGIVTRLWPN 300
           W++LANDI TG+IN KI + SVRESVM I +KPNP++AD IE EC + SWEGI+ RLWPN
Sbjct: 241 WVVLANDILTGSINPKITNLSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPN 300

Query: 301 IEYIKAIASGSMSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPN 360
            +YI AI +GSMSQYIPL+N+Y+NNLP++SDHYGSSECF GLNLDPLC P EISYTLIP 
Sbjct: 301 TKYINAIVTGSMSQYIPLLNFYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPT 360

Query: 361 VAYFEFLPVEREKSNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKN 420
           +AYFEFLP++R   NGE  Q+LVDLVDVKLGQEYELVITT+ GLYRY +GDI+RV GF  
Sbjct: 361 MAYFEFLPIDRTNPNGEVTQQLVDLVDVKLGQEYELVITTFAGLYRYRMGDIIRVTGFTK 420

Query: 421 KAPNFSFVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGH 480
           KAPNFSFVCRKN++LSLN EKT+E  LH A+++   +L PFGA +VDYTSY +++TIPGH
Sbjct: 421 KAPNFSFVCRKNVVLSLNNEKTDEAGLHKAVEEGGHVLKPFGATIVDYTSYADSSTIPGH 480

Query: 481 YVLYWEIRVEKE-----SSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVE 540
           YVLY+E+  ++      S+ IPSS+YE+C   +EE LN  YR  RS +  ++PLEI+IV+
Sbjct: 481 YVLYFELIFDEHNKAQISNSIPSSVYEDCCFAIEETLNSLYRLARSHEKCIDPLEIKIVK 540

Query: 541 CGTFDILMERAMNQGVSISQYKTPRSIKCTQ--IIELLDARVLSNHFSQKSPKWDPTR 586
            GTF+ LM+ A+N+G SI++YKTPR +   Q  II+LL++ ++SN+FSQKSPK D  R
Sbjct: 541 AGTFEKLMQLAINRGASITRYKTPRCLNSKQIHIIQLLESNLVSNYFSQKSPKLDANR 596

BLAST of Sed0018928 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 6.5e-193
Identity = 324/589 (55.01%), Postives = 431/589 (73.17%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPL 68
           L EK+K  LQ IEDVT NAD +Q++ L EILS N  VEYL++HGL G TD  TFK+++P+
Sbjct: 17  LAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPV 76

Query: 69  VSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNY 128
           V+YE ++P I+RIANGD S +LC+ PI+EF  SSGTSGGE+KL+P  +++ +R    ++ 
Sbjct: 77  VTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VIPIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQSG---GNT 188
           ++P+M Q  P   KGK M+F F ++ S T  G+ A P  T   K+S F N         T
Sbjct: 137 LMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYT 196

Query: 189 SPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRT 248
           SPN  +LC+D+YQS+Y Q+LCGLCQ++ VL++GA+F S  +  +KFL  HW  LA DIRT
Sbjct: 197 SPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRT 256

Query: 249 GTINSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGS 308
           GT++S+I DSSVRE+V  ILKP+P+LADF+E+EC + SW+GI+TRLWPN +Y+  I +G+
Sbjct: 257 GTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 316

Query: 309 MSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVER 368
           MSQYIP ++YYSN LPL+   Y SSEC+FG+NL PLCKP E+SYTLIPN+AYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 376

Query: 369 EKS-----------NGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKN 428
                           + +QELVDLVDVKLGQEYELV+TTY GLYRY VGD+L V GFKN
Sbjct: 377 NSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKN 436

Query: 429 KAPNFSFVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGH 488
            AP FSF+CRKN++LS++ +KT+E EL NA++ A   L PF A + +YTSY + ++IPGH
Sbjct: 437 NAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496

Query: 489 YVLYWEIRVEKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFD 548
           YVL+WE+ +   ++ IP S++E+C L +EE LN  YRQGR  D  + PLEI++VE GTFD
Sbjct: 497 YVLFWELCL-NGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFD 556

Query: 549 ILMERAMNQGVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDP 584
            LM+ A++ G SI+QYKTPR +K   IIELL++RV+ ++FS K PKW P
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

BLAST of Sed0018928 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 7.2e-192
Identity = 325/591 (54.99%), Postives = 435/591 (73.60%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPL 68
           LD+K+KQ LQ IE++T NAD++Q+Q L EIL+ N  VEYL++H LNG TD  TFKN++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  VSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNY 128
           ++YE ++P I+RIANGD SPIL ++PI+EF  SSGTSGGE+KL+P  +++ +R    ++ 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VIPIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQSG---GNT 188
           ++P+M Q  P    GK M+F F ++ S T  G+ A P  T   K+S F           T
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196

Query: 189 SPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRT 248
           SPN+ +LC+D+YQS+Y Q+LCGLCQ++ VL++GA+F S  +  +KFL  HWI L  DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256

Query: 249 GTINSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGS 308
           GT++S I D SVRE+V  ILKP+P+LADF+E EC + SW+GI+TRLWPN +Y+  I +G+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316

Query: 309 MSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVER 368
           MSQYIP ++YYSN LPL+   Y SSEC+FG+NL PLCKP E+SYTLIP++AYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376

Query: 369 EKSNGET-------------RQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGF 428
             +NG T             +QELVDLVDVKLGQEYELV+TTY GL RY VGD+LRV GF
Sbjct: 377 --NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGF 436

Query: 429 KNKAPNFSFVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIP 488
           KNKAP FSF+CRKN++LS++ +KT+E EL NA++ A   L PF A + +YTSY + ++IP
Sbjct: 437 KNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIP 496

Query: 489 GHYVLYWEIRVEKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGT 548
           GHYVL+WE+ ++  ++ IP S++E+C L VEE  N  YRQGR  D  + PLEI+IVE GT
Sbjct: 497 GHYVLFWELCLD-GNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGT 556

Query: 549 FDILMERAMNQGVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDP 584
           FD LM+ A++ G SI+QYKTPR +K   IIELL++RV+ ++FS K PKW P
Sbjct: 557 FDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

BLAST of Sed0018928 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 2.3e-169
Identity = 294/574 (51.22%), Postives = 396/574 (68.99%), Query Frame = 0

Query: 15  QALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTD-VSTFKNLIPLVSYEQ 74
           +AL+ IE VT NA ++Q++ L EIL+ N   EYL+++G+ GS D V  F+ L+PLV+YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 75  LKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNYVIPIM 134
           L+P I RIANGD SPI   +PI+EF  SSGTSGGE+KL+P    +  R    ++ ++P+M
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 135 KQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQSG---GNTSPNDV 194
            Q      KGK M+  F +A S T  G+ A P  T   ++  F +         TSP++ 
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201

Query: 195 LLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRTGTINS 254
           +LC D+YQS+Y QLLCGL     VL++GA+F S  +  + FL  HW  L +DIRTG ++ 
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261

Query: 255 KIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGSMSQYI 314
           +I D  VR++V  +L+ +P LAD IE EC + SWEGI+ RLWP  +YI  I +G+MSQYI
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321

Query: 315 PLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVEREKSNG 374
           P + +Y   LPL    Y SSEC+FGLNL+P+CKP +++YTLIP + Y+EFLPV    +  
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381

Query: 375 E-TRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVCRKNIIL 434
           E + ++LVDLVDVKLG EYELV+TTY GLYRY VGD+LRV GFKNKAP FSFV R+N+ L
Sbjct: 382 EASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVAL 441

Query: 435 SLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRVEKESSM 494
           S++ +KT+E ELH A+  A + L PFGA +V+YTSY +  TIPGHYVL+WE+R    S+ 
Sbjct: 442 SVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELRA--GSTA 501

Query: 495 IPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQGVSISQ 554
           +P+S++EEC L VEE LN  YRQGR+ D  + PLEIR+V  GTFD LM+ A+++G SI+Q
Sbjct: 502 VPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQ 561

Query: 555 YKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDP 584
           YK PR ++   ++ELLDARV   +FS K PKW P
Sbjct: 562 YKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSP 593

BLAST of Sed0018928 vs. ExPASy Swiss-Prot
Match: Q9LQ68 (Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=GH3.4 PE=1 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.7e-164
Identity = 279/580 (48.10%), Postives = 394/580 (67.93%), Query Frame = 0

Query: 11  EKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPLVS 70
           E + +AL+ IE++T N D +Q++ L EILS N + EYL++  LNG+ D  +FK+ +P+V 
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 71  YEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNYVI 130
           YE LK  I RI+NGD SPIL + PITEF  SSGTS GE+KL+P  ++   R     N ++
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137

Query: 131 PIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQSGG-NTSPND 190
           P+M    P   KGK ++F F ++ STT  G+ A P  T   K+  F    S    TSP +
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197

Query: 191 VLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRTGTIN 250
            +LC D+ QS+Y Q+LCGL     V +LGA+F S ++  + FL N+W  L+ DI TGT++
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257

Query: 251 SKIIDSSVRESVMNIL-KPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGSMSQ 310
           SKI D +++  + NIL KP+ +LA+F+   C Q +WEGI+T++WPN +Y+  I +G+M++
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317

Query: 311 YIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVEREKS 370
           YIP++ YYS  LP+ S  Y SSE +FG+NL+P+CKP E+SYT+ PN+AYFEFLP   +  
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377

Query: 371 NGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVCRKNII 430
            G     LV+L DV++G+EYELVITTY GLYRY VGDILRV GF N AP F F+ R+N++
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437

Query: 431 LSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRVEKESS 490
           LS+  +KT+E +L  A++ A+RLL   G  V++YTSY +  TIPGHYV+YWE+    +S+
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497

Query: 491 MIPS-SIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQGVSI 550
            +PS  +  +C L +EE LN  YRQ R  D  + PLEIR+V+ GTF+ LM+ ++++G SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557

Query: 551 SQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRRH 588
           +QYK PR +  T I++LLD+RV+S HFS   P W P RRH
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597

BLAST of Sed0018928 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 7.0e-163
Identity = 289/583 (49.57%), Postives = 394/583 (67.58%), Query Frame = 0

Query: 12  KDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPLVSY 71
           KD +AL+ IE++T N D +Q++ + EILS N   EYL++ GL G TD  TFK  +P+V Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 72  EQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNYVIP 131
           + LKP I RIANGD S IL + PITEF  SSGTS GE+KL+P  D+  +R    ++ ++P
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 132 IMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFN---NIQSGGNTSPN 191
           +M    P   KGK ++F F +  S T  G+ A P  T   K+  F    N      TSPN
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPN 197

Query: 192 DVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRTGTI 251
           + +LC D+ QS+Y Q+LCGL     VL+LGA+F S ++  + FL  +W  LA+DI TGT+
Sbjct: 198 EAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTL 257

Query: 252 NSKIIDSSVRESVMNIL-KPNPQLADFIETECGQ-RSWEGIVTRLWPNIEYIKAIASGSM 311
           +S+I D +++ES+  IL KP+ +LADFI + CGQ  SWEGI+T++WPN +Y+  I +G+M
Sbjct: 258 SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAM 317

Query: 312 SQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLP---V 371
           +QYIP++ YYS  LP+    Y SSE +FG+NL P+CKP E+SYT++PN+AYFEFLP   V
Sbjct: 318 AQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHHEV 377

Query: 372 EREKSNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVC 431
             EKS      ELV+L DV++G+EYELVITTY GL RY VGDIL+V GF N AP F FV 
Sbjct: 378 PTEKS------ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVR 437

Query: 432 RKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRV 491
           RKN++LS+  +KT+E EL +A++ A+ LL   G  V++YTSY    TIPGHYV+YWE+ V
Sbjct: 438 RKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLV 497

Query: 492 EKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQ 551
           + +++     +   C L +EE LN  YRQ R  D  + PLEIR+V+ GTF+ LM+ A+++
Sbjct: 498 KDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISR 557

Query: 552 GVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRR 587
           G SI+QYK PR +  T I+ELLD+RV+S HFS   P W P RR
Sbjct: 558 GASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERR 594

BLAST of Sed0018928 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.3e-241
Identity = 399/580 (68.79%), Postives = 478/580 (82.41%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPL 68
           LDEKDKQALQ +EDVT  AD+IQ++ L EILSTN HVEYLQQHGLNGSTD STFKNLIP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  VSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNY 128
           VSYE LKPYIDRI NGDDSPILCA PITEF+ S+GTSGGEQKLIP  +Q+F R L+  NY
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 VIPIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQ-SGGNTSP 188
           + P MKQLFP  H GKEMFF F +  S TKAGI+A   ST   K+   NN++     TSP
Sbjct: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183

Query: 189 NDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRTGT 248
            D+L CTD YQS+YCQLLCGLCQN+ V+++G+LF ST++Y+ KFL NHW+LLANDIRTGT
Sbjct: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243

Query: 249 INSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGSMS 308
           IN+KI+DS VR+SVMNILKP+PQLADFIE+EC + +WEGIVTRLWPN ++I A+ +GSMS
Sbjct: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303

Query: 309 QYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVEREK 368
            YIP +NYY  +LPLI+  Y SSECFFG+NLDPLCKPEEISYT IP VAYFEFLPV+R  
Sbjct: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363

Query: 369 SNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVCRKNI 428
            +GE  QE VDLVDVKLGQEYE+V+TTY GLYRYC+GDI+RV GFKNKAPNF FV RKN+
Sbjct: 364 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 423

Query: 429 ILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRVEKES 488
           ILSL+VEKT+E ELHNA+ +   LL+ FGA++VDYTSY +++T PGHYVLYWEI+ ++E 
Sbjct: 424 ILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEI 483

Query: 489 SMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQGVSI 548
           S+IPSSIYE+C L +EE L+  YR+ RS   ++NPLEI+IVE  TFD LMER ++QG SI
Sbjct: 484 SIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 543

Query: 549 SQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRRH 588
           SQYKTPR +K TQIIE LD++V++N+FS+K PKWDPTR H
Sbjct: 544 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583

BLAST of Sed0018928 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 3.9e-233
Identity = 392/593 (66.10%), Postives = 482/593 (81.28%), Query Frame = 0

Query: 1   MSSYEENLLDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVS 60
           M+  +E   DE+DKQALQHIEDVTI+A +IQ+Q L +ILSTN  VEYLQQHGL+GST++S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLIPLVSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFE 120
           TFKNLIPL+SYEQL+PYIDRIANGDDSPILC  PITEFFISSGTSGGE KLIPM++++F 
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RMLTFFNYVIPIMKQLFPNS--HKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNN 180
           R L+FF+YV+P MKQLFPNS   KGK M F F +    T++GI+AH  +T LCK +  N 
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN- 180

Query: 181 IQSGGNTSPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWIL 240
                NTSP D++LCTDT QS+YCQLLCGLCQN+ V ++GALF ST+++VLKFL +HW++
Sbjct: 181 -----NTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTINSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYI 300
           LA+DIR GT+N+KI D SVRE+VMNIL PN QLADFIE EC + +WEGI+TRLWPN +YI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 KAIASGSMSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYF 360
            AI +GSM+QY+P +NYYSN+LP++SDHYG+SECF GLNLDPLC P+  SYTLIP +AYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPVEREKSNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPN 420
           EFLP++R KS+ E  +ELVDLVDVKLG EYELVITTY GLYRY +GDIL V GFKNKAPN
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPN 420

Query: 421 FSFVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLY 480
           FSFVCRKN++LSL++EKT+E ELH+A+Q+AARLL+PFGA + DYTS+ +++ IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 WEIRVEKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILME 540
           WEI V K    IPSSIYE+C   +EELLN  YR  RS +  +NPLEI+IV+ GTF+ LM 
Sbjct: 481 WEIHV-KGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 541 RAMNQGVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRRHHLFS 592
           +A+N+G S+SQYKTPR +  T IIELLD+ V+  +FS+K PK D    H L S
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

BLAST of Sed0018928 vs. ExPASy TrEMBL
Match: A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 6.1e-226
Identity = 378/577 (65.51%), Postives = 473/577 (81.98%), Query Frame = 0

Query: 6   ENLLDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNL 65
           +++LD KDKQ L HIED T NAD+IQ++ + EILS N HVEYL+Q GL+GSTD+STFK L
Sbjct: 3   DDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKKL 62

Query: 66  IPLVSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTF 125
           IP+VSYEQLKPYIDRIA+GDDSPILCA  IT+F IS+GTSGG QKLIPMF  +  R L  
Sbjct: 63  IPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLAT 122

Query: 126 FNYVIPIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQ-SGGN 185
             Y IP+MKQLFP    GKEMFF FTRA + TK G++A   S+ L K S+F++++     
Sbjct: 123 NYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRAY 182

Query: 186 TSPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIR 245
           TSP D++ CTD YQS+YCQLLCGLCQN+ VL++GALF ST++Y+ KFL NHW LL NDIR
Sbjct: 183 TSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDIR 242

Query: 246 TGTINSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASG 305
           TGT+++KI D  VRESVM++LKPNP+LADFIE+EC + +WEGIVTRLWPN +YI  + +G
Sbjct: 243 TGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVTG 302

Query: 306 SMSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVE 365
           SMSQYIP +N+Y    P++S  Y SSECFFG+NLDPLCKPEEISYT +P +AYFEFLPV+
Sbjct: 303 SMSQYIPQLNFYC-KCPIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPVD 362

Query: 366 REKS-NGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVC 425
           R  S NGE  QELVDLVDVKLGQEYE+V+TTY GLYRYC+GDILRV GFKNK PNFSF C
Sbjct: 363 RANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFAC 422

Query: 426 RKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRV 485
           RKN+ILSL++EKT+E +LH+A+ +A+ LL+P GA++VDYTSY +++T PGHYVLYWEI+ 
Sbjct: 423 RKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQC 482

Query: 486 EKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQ 545
            +E+S IPS++YE+C L VEE L+  YRQ RS + ++NPLEI+IVE GTF+ LME+A+++
Sbjct: 483 REETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVDR 542

Query: 546 GVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPK 581
           G SISQYKTPR +K TQ+IELLD++V+SN+FSQ+ PK
Sbjct: 543 GASISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

BLAST of Sed0018928 vs. ExPASy TrEMBL
Match: A0A6J1CG61 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011222 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 3.0e-217
Identity = 374/583 (64.15%), Postives = 444/583 (76.16%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPL 68
           LDEKDKQ LQ++EDVT NAD+IQ++ L EILSTN HVEYL+QHGL+GSTDVSTFKNLIPL
Sbjct: 4   LDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPL 63

Query: 69  VSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNY 128
           VSYE LKPYIDRI++GDDSPILCA PIT+                               
Sbjct: 64  VSYEHLKPYIDRISSGDDSPILCAHPITD------------------------------- 123

Query: 129 VIPIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQSGGNTSPN 188
                                F RA S TK GI+A   ST L ++S FNN++SG  TSPN
Sbjct: 124 ---------------------FARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTSPN 183

Query: 189 DVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRTGTI 248
           +++ CTD YQS+YCQLLCGL Q E V+ +GALF ST+VYV KFL NHW+LLANDIRTGT+
Sbjct: 184 EIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGTV 243

Query: 249 NSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGSMSQ 308
           N+KI D  VRESVMNILKP+PQLADFIE+EC + +WEGIVTRLWPN ++I AI +GSMSQ
Sbjct: 244 NTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMSQ 303

Query: 309 YIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVEREKS 368
           YIP +NYYSN LPLIS  Y SSEC+FG+NLDPL KPEEISYT IP +AYFEFLPV+R  +
Sbjct: 304 YIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRANA 363

Query: 369 NGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVCRKNII 428
           N E  QELVDLVDVKLGQEYE+++TTY GLYRYC+GDI+RV GFK KAP FSFVCRKN++
Sbjct: 364 NREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKNVV 423

Query: 429 LSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRVEKESS 488
           LSL+ EKT+E ELHNA++ A  LL PFGA + DYTSY +N+T PGHYVLYWEI  ++E S
Sbjct: 424 LSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEEIS 483

Query: 489 MIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQGVSIS 548
           +IPSSIYE+C L VEE LN  YRQ RS   ++NPLEI++VE GTFD L+ERA  QG SIS
Sbjct: 484 IIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGASIS 534

Query: 549 QYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRRHHLFS 592
           QYKTPR +  TQI+ELLD +V++++FS+K PKWDPT RH LF+
Sbjct: 544 QYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFA 534

BLAST of Sed0018928 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.5e-213
Identity = 362/589 (61.46%), Postives = 462/589 (78.44%), Query Frame = 0

Query: 1   MSSYEENLLDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVS 60
           MS++EE  LDEKDK+ALQ+IED+T  AD+IQ+Q L EILS N +VEYLQ+HGL+ STD S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 61  TFKNLIPLVSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFE 120
           TFK LIPLV Y+QL+PYI RIA+GDDS ILC+ PITEFF SSGTSG E KLIPM++Q+F 
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 121 RMLTFFNYVIPIMKQLFPNS--HKGKEMFFCFTRAASTTKAGIIAHPTSTRLCK-TSVFN 180
           R L+FF+Y+ P MKQLFPN   HK K + F F +    TK GI+     T L K +S   
Sbjct: 121 RRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLR 180

Query: 181 NIQSGGNTSPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWI 240
           ++ SG NTSP+D++LCTD+YQS+YCQLLCGL QN+ V ++ ALF ST+++V KFL NHWI
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWI 240

Query: 241 LLANDIRTGTINSKIIDSSVRESVMNI-LKPNPQLADFIETECGQRSWEGIVTRLWPNIE 300
            LA DIRT TIN KI  SSVRESVM I +KPNP++AD IE ECG+  WEGI+TRLWPN +
Sbjct: 241 DLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 300

Query: 301 YIKAIASGSMSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVA 360
           YI A+ +GSMSQYIPL+NYY+NNLP++SDHYGSSECF GLNLDPLC P+E+SYTLIP +A
Sbjct: 301 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 360

Query: 361 YFEFLPVEREKSNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKA 420
           YFEFLP++     GE  Q LVDLVDVKLG EYELVITT+ GLYRYC+GD++RV GFKNKA
Sbjct: 361 YFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKA 420

Query: 421 PNFSFVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYV 480
           PNFSFV RKN++L+++ EKT+E  LH A+++  R++  FGA +VDYT+Y + +TIPGHYV
Sbjct: 421 PNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYV 480

Query: 481 LYWEIRV----EKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGT 540
           LYWE+ +    E+  + IP S++E+C L +E  LN+ YR  RS +  +NPLEI+IV+ GT
Sbjct: 481 LYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGT 540

Query: 541 FDILMERAMNQGVSISQYKTPRSIKCTQ--IIELLDARVLSNHFSQKSP 580
           F+ LM+ A+N+G SI+QYKTPR +  +Q  II+LL++ ++SN+FS+  P
Sbjct: 541 FEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYP 589

BLAST of Sed0018928 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 675.2 bits (1741), Expect = 4.6e-194
Identity = 324/589 (55.01%), Postives = 431/589 (73.17%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPL 68
           L EK+K  LQ IEDVT NAD +Q++ L EILS N  VEYL++HGL G TD  TFK+++P+
Sbjct: 17  LAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPV 76

Query: 69  VSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNY 128
           V+YE ++P I+RIANGD S +LC+ PI+EF  SSGTSGGE+KL+P  +++ +R    ++ 
Sbjct: 77  VTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VIPIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQSG---GNT 188
           ++P+M Q  P   KGK M+F F ++ S T  G+ A P  T   K+S F N         T
Sbjct: 137 LMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYT 196

Query: 189 SPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRT 248
           SPN  +LC+D+YQS+Y Q+LCGLCQ++ VL++GA+F S  +  +KFL  HW  LA DIRT
Sbjct: 197 SPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRT 256

Query: 249 GTINSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGS 308
           GT++S+I DSSVRE+V  ILKP+P+LADF+E+EC + SW+GI+TRLWPN +Y+  I +G+
Sbjct: 257 GTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 316

Query: 309 MSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVER 368
           MSQYIP ++YYSN LPL+   Y SSEC+FG+NL PLCKP E+SYTLIPN+AYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 376

Query: 369 EKS-----------NGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKN 428
                           + +QELVDLVDVKLGQEYELV+TTY GLYRY VGD+L V GFKN
Sbjct: 377 NSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKN 436

Query: 429 KAPNFSFVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGH 488
            AP FSF+CRKN++LS++ +KT+E EL NA++ A   L PF A + +YTSY + ++IPGH
Sbjct: 437 NAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496

Query: 489 YVLYWEIRVEKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFD 548
           YVL+WE+ +   ++ IP S++E+C L +EE LN  YRQGR  D  + PLEI++VE GTFD
Sbjct: 497 YVLFWELCL-NGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFD 556

Query: 549 ILMERAMNQGVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDP 584
            LM+ A++ G SI+QYKTPR +K   IIELL++RV+ ++FS K PKW P
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

BLAST of Sed0018928 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 671.8 bits (1732), Expect = 5.1e-193
Identity = 325/591 (54.99%), Postives = 435/591 (73.60%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPL 68
           LD+K+KQ LQ IE++T NAD++Q+Q L EIL+ N  VEYL++H LNG TD  TFKN++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  VSYEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNY 128
           ++YE ++P I+RIANGD SPIL ++PI+EF  SSGTSGGE+KL+P  +++ +R    ++ 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VIPIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQSG---GNT 188
           ++P+M Q  P    GK M+F F ++ S T  G+ A P  T   K+S F           T
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196

Query: 189 SPNDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRT 248
           SPN+ +LC+D+YQS+Y Q+LCGLCQ++ VL++GA+F S  +  +KFL  HWI L  DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256

Query: 249 GTINSKIIDSSVRESVMNILKPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGS 308
           GT++S I D SVRE+V  ILKP+P+LADF+E EC + SW+GI+TRLWPN +Y+  I +G+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316

Query: 309 MSQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVER 368
           MSQYIP ++YYSN LPL+   Y SSEC+FG+NL PLCKP E+SYTLIP++AYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376

Query: 369 EKSNGET-------------RQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGF 428
             +NG T             +QELVDLVDVKLGQEYELV+TTY GL RY VGD+LRV GF
Sbjct: 377 --NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGF 436

Query: 429 KNKAPNFSFVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIP 488
           KNKAP FSF+CRKN++LS++ +KT+E EL NA++ A   L PF A + +YTSY + ++IP
Sbjct: 437 KNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIP 496

Query: 489 GHYVLYWEIRVEKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGT 548
           GHYVL+WE+ ++  ++ IP S++E+C L VEE  N  YRQGR  D  + PLEI+IVE GT
Sbjct: 497 GHYVLFWELCLD-GNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGT 556

Query: 549 FDILMERAMNQGVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDP 584
           FD LM+ A++ G SI+QYKTPR +K   IIELL++RV+ ++FS K PKW P
Sbjct: 557 FDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

BLAST of Sed0018928 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 580.9 bits (1496), Expect = 1.2e-165
Identity = 279/580 (48.10%), Postives = 394/580 (67.93%), Query Frame = 0

Query: 11  EKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPLVS 70
           E + +AL+ IE++T N D +Q++ L EILS N + EYL++  LNG+ D  +FK+ +P+V 
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 71  YEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNYVI 130
           YE LK  I RI+NGD SPIL + PITEF  SSGTS GE+KL+P  ++   R     N ++
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137

Query: 131 PIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNNIQSGG-NTSPND 190
           P+M    P   KGK ++F F ++ STT  G+ A P  T   K+  F    S    TSP +
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197

Query: 191 VLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRTGTIN 250
            +LC D+ QS+Y Q+LCGL     V +LGA+F S ++  + FL N+W  L+ DI TGT++
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257

Query: 251 SKIIDSSVRESVMNIL-KPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGSMSQ 310
           SKI D +++  + NIL KP+ +LA+F+   C Q +WEGI+T++WPN +Y+  I +G+M++
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317

Query: 311 YIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVEREKS 370
           YIP++ YYS  LP+ S  Y SSE +FG+NL+P+CKP E+SYT+ PN+AYFEFLP   +  
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377

Query: 371 NGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVCRKNII 430
            G     LV+L DV++G+EYELVITTY GLYRY VGDILRV GF N AP F F+ R+N++
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437

Query: 431 LSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRVEKESS 490
           LS+  +KT+E +L  A++ A+RLL   G  V++YTSY +  TIPGHYV+YWE+    +S+
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497

Query: 491 MIPS-SIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQGVSI 550
            +PS  +  +C L +EE LN  YRQ R  D  + PLEIR+V+ GTF+ LM+ ++++G SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557

Query: 551 SQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRRH 588
           +QYK PR +  T I++LLD+RV+S HFS   P W P RRH
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597

BLAST of Sed0018928 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 575.5 bits (1482), Expect = 5.0e-164
Identity = 289/583 (49.57%), Postives = 394/583 (67.58%), Query Frame = 0

Query: 12  KDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPLVSY 71
           KD +AL+ IE++T N D +Q++ + EILS N   EYL++ GL G TD  TFK  +P+V Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 72  EQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNYVIP 131
           + LKP I RIANGD S IL + PITEF  SSGTS GE+KL+P  D+  +R    ++ ++P
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 132 IMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFN---NIQSGGNTSPN 191
           +M    P   KGK ++F F +  S T  G+ A P  T   K+  F    N      TSPN
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPN 197

Query: 192 DVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRTGTI 251
           + +LC D+ QS+Y Q+LCGL     VL+LGA+F S ++  + FL  +W  LA+DI TGT+
Sbjct: 198 EAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTL 257

Query: 252 NSKIIDSSVRESVMNIL-KPNPQLADFIETECGQ-RSWEGIVTRLWPNIEYIKAIASGSM 311
           +S+I D +++ES+  IL KP+ +LADFI + CGQ  SWEGI+T++WPN +Y+  I +G+M
Sbjct: 258 SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAM 317

Query: 312 SQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLP---V 371
           +QYIP++ YYS  LP+    Y SSE +FG+NL P+CKP E+SYT++PN+AYFEFLP   V
Sbjct: 318 AQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHHEV 377

Query: 372 EREKSNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFSFVC 431
             EKS      ELV+L DV++G+EYELVITTY GL RY VGDIL+V GF N AP F FV 
Sbjct: 378 PTEKS------ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVR 437

Query: 432 RKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWEIRV 491
           RKN++LS+  +KT+E EL +A++ A+ LL   G  V++YTSY    TIPGHYV+YWE+ V
Sbjct: 438 RKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLV 497

Query: 492 EKESSMIPSSIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMERAMNQ 551
           + +++     +   C L +EE LN  YRQ R  D  + PLEIR+V+ GTF+ LM+ A+++
Sbjct: 498 KDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISR 557

Query: 552 GVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRR 587
           G SI+QYK PR +  T I+ELLD+RV+S HFS   P W P RR
Sbjct: 558 GASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERR 594

BLAST of Sed0018928 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 570.1 bits (1468), Expect = 2.1e-162
Identity = 282/587 (48.04%), Postives = 393/587 (66.95%), Query Frame = 0

Query: 11  EKDKQALQHIEDVTINADKIQQQTLIEILSTNGHVEYLQQHGLNGSTDVSTFKNLIPLVS 70
           EKD +AL+ IE++T N D +Q++ L EIL+ N + EYL++  L+G  D  TFK+ +P+V+
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 71  YEQLKPYIDRIANGDDSPILCAQPITEFFISSGTSGGEQKLIPMFDQQFERMLTFFNYVI 130
           YE LKP I RI+NGD SPIL + PITEF  SSGTS GE+KL+P  ++  +R    ++ ++
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 131 PIMKQLFPNSHKGKEMFFCFTRAASTTKAGIIAHPTSTRLCKTSVFNN---IQSGGNTSP 190
           P+M    P   KGK ++F F ++ S T  G+ A P  T   K+  F           TSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 191 NDVLLCTDTYQSIYCQLLCGLCQNETVLKLGALFVSTIVYVLKFLSNHWILLANDIRTGT 250
           N+ +LC+D+ QS+Y Q+LCGL     VL+LGA+F S ++  + FL N+W  LA DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 251 INSKIIDSSVRESVMNIL-KPNPQLADFIETECGQRSWEGIVTRLWPNIEYIKAIASGSM 310
           ++S+I D +++  +  IL KP+ +LA+F+   C Q +WEGI+T++WPN +Y+  I +G+M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317

Query: 311 SQYIPLINYYSNNLPLISDHYGSSECFFGLNLDPLCKPEEISYTLIPNVAYFEFLPVER- 370
           +QYIP + YYS  LP+    Y SSE +FG+NL P+CKP E+SYT++PN+AYFEFLP    
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHD 377

Query: 371 -----EKSNGETRQELVDLVDVKLGQEYELVITTYVGLYRYCVGDILRVIGFKNKAPNFS 430
                E S  ET   LV+L +V++G+EYELVITTY GLYRY VGDILRV GF N AP F 
Sbjct: 378 GDGAAEASLDET--SLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFK 437

Query: 431 FVCRKNIILSLNVEKTEEDELHNAIQQAARLLTPFGAVVVDYTSYGNNTTIPGHYVLYWE 490
           F+ RKN++LS+  +KT+E EL  A++ A+RL    G  V++YTSY    TIPGHYV+YWE
Sbjct: 438 FIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWE 497

Query: 491 IRVEKESSMIPS-SIYEECYLVVEELLNVQYRQGRSQDNLLNPLEIRIVECGTFDILMER 550
           +    +S+ + S  +  +C L +EE LN  YRQ R  D  + PLEIR+V  GTF+ LM+ 
Sbjct: 498 LLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDY 557

Query: 551 AMNQGVSISQYKTPRSIKCTQIIELLDARVLSNHFSQKSPKWDPTRR 587
           A+++G SI+QYK PR +  T I+ELLD+RV+S HFS   P W P RR
Sbjct: 558 AISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERR 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140778.12.8e-24168.79indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia][more]
KAG6570863.16.6e-23566.27Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022944596.18.1e-23366.10indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata][more]
XP_022140368.11.3e-22565.51indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia][more]
XP_038902067.12.3e-21963.55indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LSQ46.5e-19355.01Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
O818297.2e-19254.99Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8LQM52.3e-16951.22Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
Q9LQ681.7e-16448.10Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
O221907.0e-16349.57Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Match NameE-valueIdentityDescription
A0A6J1CIS91.3e-24168.79indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ... [more]
A0A6J1FUU53.9e-23366.10indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1CHU96.1e-22665.51indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 ... [more]
A0A6J1CG613.0e-21764.15indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ... [more]
A0A5A7V3L51.5e-21361.46Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=... [more]
Match NameE-valueIdentityDescription
AT5G54510.14.6e-19455.01Auxin-responsive GH3 family protein [more]
AT4G27260.15.1e-19354.99Auxin-responsive GH3 family protein [more]
AT1G59500.11.2e-16548.10Auxin-responsive GH3 family protein [more]
AT2G23170.15.0e-16449.57Auxin-responsive GH3 family protein [more]
AT4G37390.12.1e-16248.04Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 20..557
e-value: 1.6E-180
score: 601.4
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 9..587
NoneNo IPR availablePANTHERPTHR31901:SF63INDOLE-3-ACETIC ACID-AMIDO SYNTHETASEcoord: 9..587

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0018928.1Sed0018928.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0010279 indole-3-acetic acid amido synthetase activity