Sed0018704 (gene) Chayote v1

Overview
NameSed0018704
Typegene
OrganismSechium edule (Chayote v1)
DescriptionRNA pseudouridine synthase 3, mitochondrial
LocationLG04: 418844 .. 424252 (+)
RNA-Seq ExpressionSed0018704
SyntenySed0018704
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTGCACAGCCGCCCCTTCTCACACATCTCTTTCTCTGCTGCTCTGTTCTTTTTTTTTTTGACAAAATCTGTGTATTCAAAGAACGAATGAAGTTCTTCGAAACCTATTCATCCTCGACGACTCTTGGATTGTAATAAAGGTATGAATCGTTCCGATACGCAGGTGAATCGTTTCTTTTTCACTCTGAAAACGTCCGATTCGGGCGATTTACGATTCAAAATTACGATGTGAATGGATTTCTTCACGATTCGTTTCGAATCGCACGTTTCTGAAAATGCCAATGACTTATCTGTCCGTTTTGCTGAATGTTATTAGGCTTTACTAATGACTATGCTTAGCTTGATGGGCATTTAGAATATCGAAAAACTAAGTCGCTAAACAGGCTATGTACAATATTAGGGTTTGTAACGAGTAAACGGGCTATCACAAATTTTGGCGAATGCTAGCCATATTGCTATGGCGTAGCTAGTATGGTTCAAACATGGTTTTGAAAGGGGCGTGTAGTCATAGATATTGAATGAAAATCTCATAGGTGGAGAACAAGGACATTCAATTGAATATATGTCCGTCTTTCATTTAATTTTTCCTTTGATATCAATCTTCAAATGTTGTTGAATTAAAAAAAGAAATTATCTCTTGATATTTGTTGGTGTTTGGGCTACCTTACACGCACCCGACTATTCTGACGGGACAACCTTTGGACCATACTACATTTGGCTATCTAAGTAAACCCGTAAAAAATTAGTTCTTAGGTAGGTGGTCACCTTCGTTTATTATTTATTTTGCTATTGAACCCTTTTATTTGGAGGCATTTTGAATAAGTGTATGGAGATGGTGCATTTTTTTATATAAAAAACCTTTCTAATAATTGCAGACTATAGCCATCAGGGGATGTTTTGCTCGAAAGATTACGGTATTATGCGCCAGAGCTTACGACGATATCTTTTGGTTGCAGCCAGATACTACTCTAGAATTTCTCCCCCACCACCTGCCTATGCTAAACCTATCATTAGAGTTTCCAACAACATAGCACAATTGGGTAGTCCTAAAGAAGGCCCAAAGCCTCGACAACTTCTATCCTTGCCCCCATTTCCTGGTTACTCTCTGCCTGGGAAGAATCATATTGGTGTCTCTGGGGAGTCAACTCATGTCACTGCCATAAAATGGGTTAAACATTACTTTGATGAAATTGACGATCTTGTAGTTCACTCGCATTTCAGAGAAGGTCTTGTAAGTATGAACGTTTACTCCTCTTCTAGCTTATTGGTTTTGTCTTTTTTCTGCTATGCTTATAAGTTATAACTAGTGTTGTAGGTTTCATGTAACTTTTATCATGTCGAGAATACCCATTCATCTTTTTGAGCAGTGAGACCAGTTCGGACTTGTTATTAGTAGCGGTAACTTAAACTCTTGTTTGTTTAAAGAAATTAAGTTCCATGCTTTCTTGTGTTATTATTATAAATAAATGTATATTGTGCTGTGGGAAAAAAGTATGTTGGACTAATTTTCTTGTAGAATTTCGGTTCATGGGAAAAACTTAAGTAATGACTTGAGTAGATGGATGTCCAAGGATATGCCACAATTTCAAGTTATGAGTTATGAATTTGACCCCTCTCAATAAGAAAAAAAAATCAAATCGGGGGGATTGGTAGTTATCTGATCAGTTAAGGGTTTATATCTTCCTTTTTTCAGGTCCGGGTGGAGTGTCCACAATCAAGTTGCTCGGACGGAAAAGGAAATTTGAAACCAATGAGAAAGGTATTGTTTCTTGAATAAATAGCCTATTCTCTCATGTTTCATTTGGAATTTCAATGTGGACATTTTTATAAGCTCCTGATCTGTTGTTTATTATGCCCACACAATATCTGGTTTTGTTTTCTTATTGAAGTCATTGTTTGTTGGTCTATTAACTTGCTACCAGATGGGTTTCAGATTTGGGGTAACGAGATTATGGAAACTGGGGCAAGAATCCATATACCTGTGTCAGTTGCAGAGATGAAGATTTCTAGGAGATTTGACACTATACCAAGTGGCACATTGTATCCAAACGCTGATGAAATCAAGTACTTGCAAAGGCTTGTCAAGTACAAGGTATGTATTGAGTTTTAACTTTCATTCTCCTATTTTCTGTTTGCTACAATGAATTCATCTCTCGGGTTCTGCCCATTGATATACAAGAAGCAGTTGATATATAATTGGTGTGGAAAGATCCACATAGATGTACTCATTTTCGTGTGTAAAACATACCTTGGTTGAGTTTGATCATTAATATGATATTTGTTTTATGCTTGGCATGATTTCATTTGATTGTTAACTTTGTGTTATTTCAGGATTCTGCTATTATCGTATTAAATAAACCTCCAAAACTACCTGTCAAGGTTTACCGCTCATTCTCTTCTATTTTCCTATGTTATGTCTGGTGGTGGGTCGGTGGGGCGGAGGTTATACTGAAATTTTGAAACAATTGTTGAATTGTTAGGGAAATCTTCCAGTTCATAATAGTATGGATGCGTTGGCGGCTGCAGCATTATCTTATGATTATGATGATGGTCCAAAGTTGGTAAGCTTCCTGTTTACTTTTTATTAACTTATGTAGGTATTGCTATTAGAAGATTCTGTGTCGAGGATGAAAATAGCCTAGTAAGTATATTGATATGCATTTGATGAATACTTATGGAAATAAGATGGGTTTATGCCATAGCCATGCGACTACGTTGTGATTCAATTAGAAAGACTGGATGGGATGAATGATAAAAAGTATTTCCTTTTAAGTTGTAGCTATGACAAAGTATTTGTCTCTTATACATGGATAATTTTTTATGGATATTTCATTTAACTTAAAAAGTTAAAAAGAAATTTAAGCCCCACCAACAAGAGTATAGCTCATTTATGACCAAGGGGCCATAGATTCGAATCCTCCCACACTCACTTATTGTTGAATTAAAAAAGAAATTAGAACCTCATTTGATAACCATTTAATTTTGATTTTTGAAAATTCTCTTAATTTCTTATTGTCTTTCTTAATGGAAAGTTTAAAGATGTTTCTTTCTAAAAACAAATACTAAAAATCAAACGGCAGTTTCAGGTTTTTCATTTCAAGAAACTGTGTTTATTCTTTAGGACGTAGAAGCTAATATCTTTTCTTATTTTTCATCTGCTATGGCATCTTTGTTTATTCTTTAGGTTCATCGTTTGGATAGAGAAAGCAGCGGGATTCTCTTGATGGGGAGAACAGAAGAAAGTGTAGCCCACCTGCAATGGCTTTTTAGCAACATTAGAAATGGAAAATCTTCATGTAAGGTGCTTATTGCCAATATGCATAGAAACTTAAACAAAAAGCTAATAAGAAGATTAAACTGAGCCGTGAGGCTTATCTTCAAGTTACCAATTACTTCGAAGCCATGGCTAAGAAATTTCGTAGAATTTCTAATTATTTGCAGTTTTTTTTTAATATGCTTGACAACGATTTTAGGCTTGGAACGAGGCCTCTGATGCGACATATCAGAAGTATTGGGCGTTAGTAATTGGTTGTCCCAAAGAAAGAGAAGGCTTAATTTCTGCTCCTCTTTCAAAGGTTCTCTCTCCCCCTCCCGGATCGATATTCGTGATTTTCTAGCTTTTCTGGTAGATTAATGTATTTCAGAATTGTTAACTAGGTCCCCTAGGGGCGGAGTAGTAATTGAACGCCTAGATTTTGGAGGTGTGCTCCCCTCAAGGTCCCAGAGGTGACTGTGGGGATTAATTTCCCAGGCTACCCAATCTCATTTCTGATCCTACAACGTATCTTGGTGCGACAGTCTAAAGACAGGTCAGTGGCGCCTTGAGTTTAAAAAAGAAAAGCTCCAAGTTCTCAGGTCATTAGAAAAAAGTTAATTAGAGATCAATATTTTTTCATTAGTTGATTTTTAGAATACTATAATGTACCATACCAGAGGTACAATGGTTAGTTGGTCTGAATTATGTTCAGTAACAAACTGTTCGATACCTGTTCTAAAACTCGAGAATACGGGTCAAACGAGTTTTTTGAGGATTAGCCCGGGTCGTTCTAATAGTGTTATAATTGATTATAGGTGCTTCTTGATGATGGGAAGACCGAGAGAGTTGTCTTGGCAAACAGAACATCCATGGAACCTTATCAGGAGGCAATCACTGAATATCGTGTGCTTGGTCCCACTATAAATGGTTGTTCTTGGATTGAACTGTGTCCGCGTACTAGCCGAAAGCATCAGGTACTTCAAGCTCTCTCTCTTCCATAACCTAATTAAAATTCATGCATATATTAACTGTATCAACTATTATGGATTGGCCTAATGGTCAAAGAGGGCTTTGGAGATTCAAGAGGCTATGGATTCAATCCATGGTGGCTATCCATGAATCTATTTTCTACAAGTTTCCTTGACATCCAAATGTTGTAGCCTTTGTAGGGTCAGACAATATGTCTCAAAAAAATAATTGAGGTATGCACAAGTTAGTCGAAATACTCACGGATATTAAAAAAAAATTAACGGTGTCAAGTTCGTGGGTTGGGCGAGGGCCTTAAGAATTAAGTGATTATGGGTTCAGTCCATTTTCTCGCCCCCTATTAAAGAATTACTTTCTTACGAAATTTCTTGACAATCAAATATTGTAGGGTCAGACTTGTGAGAATAGTTAGATGTGCACATACGCTGCCTTAACAATCACAAAATATATATATATATATATGTATATATTAACTGTACAACTTTAGACTTTAGTATCGATCCTGTGAACCATGAAGGGAATTGTTAATTTACATTCTTCTTATTCTTTATATAGATAAGAACTCTTTTCTGTTCGAAAGTAACGAATCCATCTTTCAGCTACGAATCCATTGTGCTGAAGCTCTGGGCACTCCTATTGTTGGCGACTACAAATACGGTTGGTTTGTACATCGATCATGGAAACAAATGCCACGGGTCGACGTTGAGCCGATAAGTGGGAGGCCGTACAAGTTACGGAGGCCAGAAGGGCTGAGTGTGCAGAAGGGGAGTGTTTTATCCAAGGTGCCTTTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTCCTCCCTGTTTTGGATGATAAAAGGTCGATCGGCCAGCACCCGATATCCCGCGTGGACTCGGATCTCTTACGCTTTGTCGCGAAAATGCCTTCTCACATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTGTGAGCGATGGGAATATGAAAAATTGAGCCAGAATTATCTGTCTTTGGATTATTTATTTGTTTATTTTGATATAAACATGAACATTAACAACCTCTCTAAACAGCGAAAATGTATAATTATCAATATGAACGAAAGTGGTTAGGTGGTTAGGTGGTCATCAATTTAGTTGGTGCTTTTATTTATTAGAATGAA

mRNA sequence

TCTGCACAGCCGCCCCTTCTCACACATCTCTTTCTCTGCTGCTCTGTTCTTTTTTTTTTTGACAAAATCTGTGTATTCAAAGAACGAATGAAGTTCTTCGAAACCTATTCATCCTCGACGACTCTTGGATTGTAATAAAGACTATAGCCATCAGGGGATGTTTTGCTCGAAAGATTACGGTATTATGCGCCAGAGCTTACGACGATATCTTTTGGTTGCAGCCAGATACTACTCTAGAATTTCTCCCCCACCACCTGCCTATGCTAAACCTATCATTAGAGTTTCCAACAACATAGCACAATTGGGTAGTCCTAAAGAAGGCCCAAAGCCTCGACAACTTCTATCCTTGCCCCCATTTCCTGGTTACTCTCTGCCTGGGAAGAATCATATTGGTGTCTCTGGGGAGTCAACTCATGTCACTGCCATAAAATGGGTTAAACATTACTTTGATGAAATTGACGATCTTGTAGTTCACTCGCATTTCAGAGAAGGTCTTGTCCGGGTGGAGTGTCCACAATCAAGTTGCTCGGACGGAAAAGGAAATTTGAAACCAATGAGAAAGATTTGGGGTAACGAGATTATGGAAACTGGGGCAAGAATCCATATACCTGTGTCAGTTGCAGAGATGAAGATTTCTAGGAGATTTGACACTATACCAAGTGGCACATTGTATCCAAACGCTGATGAAATCAAGTACTTGCAAAGGCTTGTCAAGTACAAGGATTCTGCTATTATCGTATTAAATAAACCTCCAAAACTACCTGTCAAGGGAAATCTTCCAGTTCATAATAGTATGGATGCGTTGGCGGCTGCAGCATTATCTTATGATTATGATGATGGTCCAAAGTTGGTTCATCGTTTGGATAGAGAAAGCAGCGGGATTCTCTTGATGGGGAGAACAGAAGAAAGTGTAGCCCACCTGCAATGGCTTTTTAGCAACATTAGAAATGGAAAATCTTCATGTAAGGCTTGGAACGAGGCCTCTGATGCGACATATCAGAAGTATTGGGCGTTAGTAATTGGTTGTCCCAAAGAAAGAGAAGGCTTAATTTCTGCTCCTCTTTCAAAGGTGCTTCTTGATGATGGGAAGACCGAGAGAGTTGTCTTGGCAAACAGAACATCCATGGAACCTTATCAGGAGGCAATCACTGAATATCGTGTGCTTGGTCCCACTATAAATGGTTGTTCTTGGATTGAACTGTGTCCGCGTACTAGCCGAAAGCATCAGCTACGAATCCATTGTGCTGAAGCTCTGGGCACTCCTATTGTTGGCGACTACAAATACGGTTGGTTTGTACATCGATCATGGAAACAAATGCCACGGGTCGACGTTGAGCCGATAAGTGGGAGGCCGTACAAGTTACGGAGGCCAGAAGGGCTGAGTGTGCAGAAGGGGAGTGTTTTATCCAAGGTGCCTTTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTCCTCCCTGTTTTGGATGATAAAAGGTCGATCGGCCAGCACCCGATATCCCGCGTGGACTCGGATCTCTTACGCTTTGTCGCGAAAATGCCTTCTCACATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTGTGAGCGATGGGAATATGAAAAATTGAGCCAGAATTATCTGTCTTTGGATTATTTATTTGTTTATTTTGATATAAACATGAACATTAACAACCTCTCTAAACAGCGAAAATGTATAATTATCAATATGAACGAAAGTGGTTAGGTGGTTAGGTGGTCATCAATTTAGTTGGTGCTTTTATTTATTAGAATGAA

Coding sequence (CDS)

ATGTTTTGCTCGAAAGATTACGGTATTATGCGCCAGAGCTTACGACGATATCTTTTGGTTGCAGCCAGATACTACTCTAGAATTTCTCCCCCACCACCTGCCTATGCTAAACCTATCATTAGAGTTTCCAACAACATAGCACAATTGGGTAGTCCTAAAGAAGGCCCAAAGCCTCGACAACTTCTATCCTTGCCCCCATTTCCTGGTTACTCTCTGCCTGGGAAGAATCATATTGGTGTCTCTGGGGAGTCAACTCATGTCACTGCCATAAAATGGGTTAAACATTACTTTGATGAAATTGACGATCTTGTAGTTCACTCGCATTTCAGAGAAGGTCTTGTCCGGGTGGAGTGTCCACAATCAAGTTGCTCGGACGGAAAAGGAAATTTGAAACCAATGAGAAAGATTTGGGGTAACGAGATTATGGAAACTGGGGCAAGAATCCATATACCTGTGTCAGTTGCAGAGATGAAGATTTCTAGGAGATTTGACACTATACCAAGTGGCACATTGTATCCAAACGCTGATGAAATCAAGTACTTGCAAAGGCTTGTCAAGTACAAGGATTCTGCTATTATCGTATTAAATAAACCTCCAAAACTACCTGTCAAGGGAAATCTTCCAGTTCATAATAGTATGGATGCGTTGGCGGCTGCAGCATTATCTTATGATTATGATGATGGTCCAAAGTTGGTTCATCGTTTGGATAGAGAAAGCAGCGGGATTCTCTTGATGGGGAGAACAGAAGAAAGTGTAGCCCACCTGCAATGGCTTTTTAGCAACATTAGAAATGGAAAATCTTCATGTAAGGCTTGGAACGAGGCCTCTGATGCGACATATCAGAAGTATTGGGCGTTAGTAATTGGTTGTCCCAAAGAAAGAGAAGGCTTAATTTCTGCTCCTCTTTCAAAGGTGCTTCTTGATGATGGGAAGACCGAGAGAGTTGTCTTGGCAAACAGAACATCCATGGAACCTTATCAGGAGGCAATCACTGAATATCGTGTGCTTGGTCCCACTATAAATGGTTGTTCTTGGATTGAACTGTGTCCGCGTACTAGCCGAAAGCATCAGCTACGAATCCATTGTGCTGAAGCTCTGGGCACTCCTATTGTTGGCGACTACAAATACGGTTGGTTTGTACATCGATCATGGAAACAAATGCCACGGGTCGACGTTGAGCCGATAAGTGGGAGGCCGTACAAGTTACGGAGGCCAGAAGGGCTGAGTGTGCAGAAGGGGAGTGTTTTATCCAAGGTGCCTTTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTCCTCCCTGTTTTGGATGATAAAAGGTCGATCGGCCAGCACCCGATATCCCGCGTGGACTCGGATCTCTTACGCTTTGTCGCGAAAATGCCTTCTCACATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTGTGA

Protein sequence

MFCSKDYGIMRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQSSCSDGKGNLKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTEESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDDGKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCRELVLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV
Homology
BLAST of Sed0018704 vs. NCBI nr
Match: XP_022131827.1 (RNA pseudouridine synthase 3, mitochondrial isoform X3 [Momordica charantia])

HSP 1 Score: 886.7 bits (2290), Expect = 8.8e-254
Identity = 424/473 (89.64%), Postives = 451/473 (95.35%), Query Frame = 0

Query: 10  MRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 69
           M+QS+RR+LL+AARYYSRISPPPPAYAKP+IRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 70  YSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQSSCSDGKGN 129
           Y LP KNHIG+SG STHVTAI WVKHYFDEI D V+ SHFR+GL++VECPQS  S+GKGN
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120

Query: 130 LKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 189
           LKPMRKI  NEIMETGAR+HIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 190 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTE 249
           SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGILLMGRT+
Sbjct: 181 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTK 240

Query: 250 ESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDD 309
           ESVAHLQWLFSNIRN KSSCKAW+EA +ATYQKYWALVIGCP+EREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 310 GKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 369
           GKTERVVLA++TS+E  QEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 370 IVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCREL 429
           IVGDYKYGWFVHR+WKQMPRVDVE ISG+PYKLRRPEGL VQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420

Query: 430 VLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           VLPNIAKF+ VL +KRSI QHP+S  +SDLLRFVA+MPSHMRISWNLMSSYLV
Sbjct: 421 VLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Sed0018704 vs. NCBI nr
Match: XP_022997125.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima] >XP_022997126.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima])

HSP 1 Score: 877.5 bits (2266), Expect = 5.4e-251
Identity = 416/473 (87.95%), Postives = 448/473 (94.71%), Query Frame = 0

Query: 10  MRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 69
           MRQS+RR+LL+AARYYSRISPPPPAYA+P+IRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLAARYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 70  YSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQSSCSDGKGN 129
           Y LP KN IG SGESTHVTAI WVKHYFDEI D V+ SHFR+GLV+V+CPQS   DG+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 130 LKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 189
           LKPMRKI  NEIMETGAR+HIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 190 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTE 249
           SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGI+LMGRT+
Sbjct: 181 SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRTK 240

Query: 250 ESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDD 309
           ESVAHLQWLFSNIRN KSSCKAW+EA +ATYQKYWALVIGCP+EREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 310 GKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 369
           GKTERVVLAN+TS E  QEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKTSFEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 370 IVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCREL 429
           IVGDYKYGWFVHR+WKQMPRVD+EPISG+PYKLRRP+GL VQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCREL 420

Query: 430 VLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           VLPN+AKFL VLDDKRSI  H +S ++SDLLRFVA+MPSHMRISWNLMSSYLV
Sbjct: 421 VLPNLAKFLNVLDDKRSISHHRVSNLNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Sed0018704 vs. NCBI nr
Match: XP_022961737.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata] >XP_022961738.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata])

HSP 1 Score: 876.3 bits (2263), Expect = 1.2e-250
Identity = 415/473 (87.74%), Postives = 448/473 (94.71%), Query Frame = 0

Query: 10  MRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 69
           MRQS+RR+LL+A RYYSRISPPPPAYA+P+IRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 70  YSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQSSCSDGKGN 129
           Y LP KN IG SGESTHVTAI WVKHYFDEI+D V+ SHFR+GLV+V+CPQS   DG+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIEDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 130 LKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 189
           LKPMRKI  NEIMETGAR+HIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 190 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTE 249
           SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGI+LMGRT+
Sbjct: 181 SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRTK 240

Query: 250 ESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDD 309
           ESVAHLQWLFSNIRN KSSCKAW+EA +ATYQKYWALVIGCP+EREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 310 GKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 369
           GKTERVVLAN+TS+E  QEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 370 IVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCREL 429
           IVGDYKYGWFVHR+WKQMPRVD+EPISG+PYKLRRP+GL VQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCREL 420

Query: 430 VLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           VLPN+AKFL VLDDKRSI  H +S  +SDLLRFVA+MPSHMRISWNLMSSYLV
Sbjct: 421 VLPNLAKFLNVLDDKRSISHHRVSYFNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Sed0018704 vs. NCBI nr
Match: XP_038885249.1 (LOW QUALITY PROTEIN: RNA pseudouridine synthase 3, mitochondrial [Benincasa hispida])

HSP 1 Score: 872.1 bits (2252), Expect = 2.3e-249
Identity = 417/482 (86.51%), Postives = 450/482 (93.36%), Query Frame = 0

Query: 1   MFCSKDYGIMRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQ 60
           +FC K Y IM Q++ R+LL+A RYYSRISPPPP+YAKP+IRVSNNIAQLGSPKEGPKPRQ
Sbjct: 36  LFCLKGYRIMWQNI-RHLLLATRYYSRISPPPPSYAKPVIRVSNNIAQLGSPKEGPKPRQ 95

Query: 61  LLSLPPFPGYSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQ 120
           LLSLPPFPG+ LPGKN IGVSGESTHVTAI+WVKHYFDEI+D V+ SHFR+GLV+VEC +
Sbjct: 96  LLSLPPFPGHPLPGKNQIGVSGESTHVTAIRWVKHYFDEIEDSVIQSHFRKGLVQVECHK 155

Query: 121 SSCSDGKGNLKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKY 180
               D KGN KPMRKI  +EI+ETGARIHIPVSVAE+K+SRRFDTIPSGTLYPNADEIKY
Sbjct: 156 XGGLDVKGNSKPMRKIRSSEIVETGARIHIPVSVAELKVSRRFDTIPSGTLYPNADEIKY 215

Query: 181 LQRLVKYKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESS 240
           LQRLVKYKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESS
Sbjct: 216 LQRLVKYKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESS 275

Query: 241 GILLMGRTEESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISA 300
           GILLMGRT+ESVAHLQWLFSNIRN +S+CKAW+EA +ATYQKYWALVIGCP+EREGLISA
Sbjct: 276 GILLMGRTKESVAHLQWLFSNIRNARSACKAWDEACNATYQKYWALVIGCPREREGLISA 335

Query: 301 PLSKVLLDDGKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRI 360
           PLSKVLLDDGKTERVVLAN+TS+E  QEAITEYRVLGPTINGCSWIELCP TSRKHQLRI
Sbjct: 336 PLSKVLLDDGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPHTSRKHQLRI 395

Query: 361 HCAEALGTPIVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVP 420
           HCAEALGTPIVGDYKYGWFVHR+WKQMPRVDVEPISG+PYKLRRP GL VQKGSVLSKVP
Sbjct: 396 HCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEPISGKPYKLRRPAGLDVQKGSVLSKVP 455

Query: 421 LLHLHCRELVLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSY 480
           LLHLHCRELVLPN AKF+ VLDDKR I  HP+S ++SDLLRFVA+MPSHMRISWNLMSSY
Sbjct: 456 LLHLHCRELVLPNFAKFVHVLDDKRPISHHPVSSLNSDLLRFVARMPSHMRISWNLMSSY 515

Query: 481 LV 483
           LV
Sbjct: 516 LV 516

BLAST of Sed0018704 vs. NCBI nr
Match: XP_023545207.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023545209.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 869.4 bits (2245), Expect = 1.5e-248
Identity = 412/473 (87.10%), Postives = 445/473 (94.08%), Query Frame = 0

Query: 10  MRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 69
           M QS+RR+LL+A RYYSRISPPPPAYA+P+IRVSNN+AQLGS KEG KPRQLLSLPPFPG
Sbjct: 1   MWQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSRKEGSKPRQLLSLPPFPG 60

Query: 70  YSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQSSCSDGKGN 129
           Y LP KN IG SGESTHVTAI WVKHYFDEI D V+ SHFR+GLV+V+CPQS   DG+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 130 LKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 189
           LKPMRKI  NEIMETGAR+HIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 190 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTE 249
           SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGI+LMGRT+
Sbjct: 181 SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRTK 240

Query: 250 ESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDD 309
           ESVAHLQWLFSNIRN KSSCKAW EA +ATYQKYWALVIGCP+EREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWGEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 310 GKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 369
           GKTERVVLAN+TS+E +QEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKTSLEAFQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 370 IVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCREL 429
           IVGDYKYGWFVHR+WKQMPRVD+EPISG+PYKLRRP+GL VQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCREL 420

Query: 430 VLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           VLPN+AKFL VLDD RSI  H +S ++SDLLRFVA+MPSHMRISWNLMSSYLV
Sbjct: 421 VLPNLAKFLNVLDDTRSISHHRVSNLNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Sed0018704 vs. ExPASy Swiss-Prot
Match: Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 3.2e-190
Identity = 321/472 (68.01%), Postives = 387/472 (81.99%), Query Frame = 0

Query: 17  YLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYSLPGKN 76
           YL V  R YSR++PPP +    +IRV+NN+A LG PK+GP PRQL+SLPPFPG+ LPGKN
Sbjct: 12  YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71

Query: 77  HIGVSGE----STHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVEC--PQSSCSDGKGNL 136
             G  G+      HVTAI WVK+YF+EI D  + +HF +GLV++E    + +  + +   
Sbjct: 72  -AGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDGA 131

Query: 137 KPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKDS 196
            PMRKI  NE+M+ G +I +PVS+AEM+IS+R+DTIPSGTLYPNADEI YLQRLV++KDS
Sbjct: 132 IPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKDS 191

Query: 197 AIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTEE 256
           AIIVLNKPPKLPVKGN+P+HNSMDALAAAALS+  D+GP+LVHRLDRE+SG+L+MGRT+E
Sbjct: 192 AIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRTKE 251

Query: 257 SVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDDG 316
           S+ +L  +FS+ +   SSCKAWN+A +A YQ+YWALVIG PKE+EGLISAPLSKVLLDDG
Sbjct: 252 SIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLDDG 311

Query: 317 KTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPI 376
           KT+RVVLA  +  E  Q+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGTPI
Sbjct: 312 KTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGTPI 371

Query: 377 VGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCRELV 436
           VGDYKYGWFVH+ WKQMP+VD+EP +G+PYKLRRPEGL VQKGSVLSKVPLLHLHCRE+V
Sbjct: 372 VGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREMV 431

Query: 437 LPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           LPNIAKFL V++ + +   H       DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 LPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

BLAST of Sed0018704 vs. ExPASy Swiss-Prot
Match: Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 7.5e-171
Identity = 295/476 (61.97%), Postives = 365/476 (76.68%), Query Frame = 0

Query: 15  RRYLLVAARYYSRISPPPPAYAKP-IIRV-SNNIAQLGSPKEGPKPRQLLSLPPFPGYS- 74
           RR +  A R+ SR++PP PA A P ++RV  +N+A+LG PK GP+PRQLLSLPPFPG   
Sbjct: 5   RRRVGAAVRWLSRLAPPAPAEADPVVVRVDGSNVARLGKPKPGPRPRQLLSLPPFPGGGD 64

Query: 75  ---LPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECP--QSSCSDG 134
              LPG+     +     VTA+ WVKHY  ++   VV +HF + LV  EC   + S    
Sbjct: 65  GDPLPGRK----AAAPRRVTAVSWVKHYLADVPQEVVQAHFNKRLVYSECSDHEVSVETI 124

Query: 135 KGNLKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVK 194
           K     ++KI  N++ME G RIH+PVSVAE +I +R++TIP+ TL+PN DEI+YL+RLV 
Sbjct: 125 KSQKHHLKKIKHNDVMEPGMRIHLPVSVAEGEIKKRYETIPTATLHPNKDEIEYLRRLVI 184

Query: 195 YKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMG 254
           +KDSAI+VLNKPPK+P+KGNLPVHNSMD LAAAALSY  ++GPKLVHRLDRESSG+LL G
Sbjct: 185 HKDSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKLVHRLDRESSGLLLFG 244

Query: 255 RTEESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVL 314
           RT+ES   L WLF+++   K++ + WN A +A  Q+YWALVIG PKEREG+ISAPLSKVL
Sbjct: 245 RTKESFTRLHWLFTSVNLAKTNSQVWNAACEAYMQRYWALVIGTPKEREGIISAPLSKVL 304

Query: 315 LDDGKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEAL 374
           LDDGK ERV+LA+ + ++  QEA+T YRV+GPTI+GCSWIEL P T RKHQLR+HCAEAL
Sbjct: 305 LDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRPLTGRKHQLRVHCAEAL 364

Query: 375 GTPIVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHC 434
           GTPIVGDYKYGWFVH+ WKQ P+ D EP +G PYKLRRPEGL +QKGSVLSKVPLLHLHC
Sbjct: 365 GTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLEIQKGSVLSKVPLLHLHC 424

Query: 435 RELVLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           RE+V+PNIAKFL    +    G  P S+   +LLRF+A MP+HM+ISWN+MSSYLV
Sbjct: 425 REMVIPNIAKFLSSNGEWHENGA-PWSKEKPNLLRFIAPMPAHMKISWNIMSSYLV 475

BLAST of Sed0018704 vs. ExPASy Swiss-Prot
Match: Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 7.2e-73
Identity = 161/427 (37.70%), Postives = 241/427 (56.44%), Query Frame = 0

Query: 62  LSLPPFPGY---------SLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREG 121
           L+LPPF             L   + +  S +++  TA++W+     ++   +V   FR  
Sbjct: 56  LTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFRLR 115

Query: 122 LVRVECPQSSCSDGKGNLKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLY 181
            VR E   S   D +     ++++   E +  G RI++P+SV     + +       +  
Sbjct: 116 QVRREMSMSVDGD-ELQRSQLKRVAAKESLNVGDRIYLPLSVD--NDTPQTPPAKKESFQ 175

Query: 182 PNADEIKYLQRLVKYKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLV 241
            + +E K++  LV YKD AIIVLNKP  L V+G   +  S+D LAA+ L +D  + P+LV
Sbjct: 176 CSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLV 235

Query: 242 HRLDRESSGILLMGRTEESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPK 301
           HRLDR+ SG+L++ RT+ +   L  +F     G S+         +  +KY ALVIGCP 
Sbjct: 236 HRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGVKKNVK-SLKRKYMALVIGCPP 295

Query: 302 EREGLISAPLSKVLLDDGKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRT 361
            + G ISAPL KV++DDGK+ER+ + +   +   Q AITEYRV+  + +G +W+EL P T
Sbjct: 296 RQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLT 355

Query: 362 SRKHQLRIHCAEALGTPIVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQK 421
            RKHQLR+HCAE LGTPIVGDYKYGW  H++ +     +  P      +   P GL +  
Sbjct: 356 GRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSSENNPTK----QSSSPFGLDLDG 415

Query: 422 GSVLSKVPLLHLHCRELVLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRI 480
           G V SK P LHLH +++ LPNI++   +L+  +      IS +DS  L+F A +PSHM++
Sbjct: 416 GDVSSKQPHLHLHSKQIDLPNISQ---LLEKMQVSSDSDISDLDS--LKFDAPLPSHMQL 469

BLAST of Sed0018704 vs. ExPASy Swiss-Prot
Match: Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.1e-44
Identity = 107/304 (35.20%), Postives = 166/304 (54.61%), Query Frame = 0

Query: 62  LSLPPFPGYSLPGKNHI-----GVSGESTH-VTAIKWVKHYFDEIDDLVVHSHFREGLVR 121
           L LPPF                G  G+     TAIKWV+    ++   +V   FR   V+
Sbjct: 53  LQLPPFAPLDAAAAARAISRGGGEGGDGEQGATAIKWVRRCCPDLPTSLVQKLFRLRKVK 112

Query: 122 --VECPQSSCSDGKGNLKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYP 181
             V   + S +D       +R++   + +  G  + +PV++ E  ++ +         + 
Sbjct: 113 KNVVTAEISSADASAEQHRLRRVSAKDQLMPGDILFLPVNLKESSVAEKTKK------FD 172

Query: 182 NADEIKYLQRLVKYKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVH 241
           N +EI +L+ L  YKD AIIV+NKPP +PV+G + + NS+D L A+    +  + P+LVH
Sbjct: 173 NRNEINFLRGLEIYKDEAIIVVNKPPGMPVQGGVGIKNSIDVL-ASMFEENSSEAPRLVH 232

Query: 242 RLDRESSGILLMGRTEESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKE 301
           RLDR+ SGIL++GR + S + L  +F      K++    +       +KY ALVIG P+ 
Sbjct: 233 RLDRDCSGILVLGRNQLSTSMLHAIFRE----KTADALADGTQHVLQRKYVALVIGTPRH 292

Query: 302 REGLISAPLSKVLLDDGKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTS 358
            +GL+SAPL+K+LL DGK+ER+ +   ++    Q+A+TEYRV+     G +W+EL PRT 
Sbjct: 293 PKGLLSAPLAKILLQDGKSERLTIRASSNAASVQDALTEYRVIESCPQGYTWLELFPRTG 345

BLAST of Sed0018704 vs. ExPASy Swiss-Prot
Match: Q28C59 (Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN=rpusd4 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 7.8e-27
Identity = 93/292 (31.85%), Postives = 137/292 (46.92%), Query Frame = 0

Query: 174 NADEIKYLQRL---VKYKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYD---D 233
           NA  I YL+ L   V  +D  ++++NKP  LPV G   V  S+ +L  A   + +    +
Sbjct: 37  NAPTIVYLRELKSQVVREDPELVLVNKPHGLPVHGGPTVERSVASLLPALAKHHFGWKAE 96

Query: 234 GPKLVHRLDRESSGILLMGRTEESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALV 293
             KL HRLDR+++G L++ RT E+   +Q             +A  E     ++ YWAL 
Sbjct: 97  PLKLCHRLDRDTTGALILARTTEAAERVQ-------------QALRERE--VHRVYWALC 156

Query: 294 IGCPKEREGLISAPLSKV------------------LLDDGKTERVVLANRTSMEPYQEA 353
           +G P  REG++  P+ +                   + ++G  ERV +          EA
Sbjct: 157 LGTPSPREGILDIPIMEKETSGPQKHYKMALSPRFRVSEEGAVERVRVPRSA-----HEA 216

Query: 354 ITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRSWKQMPR 413
           +T YR LG   +G S +EL P T  KHQLR+H A  L  PI+GD+KY  +  R   Q P 
Sbjct: 217 VTRYRTLG-AASGASLVELHPITGVKHQLRVHLALGLNCPILGDHKYSHW-GRLAPQKP- 276

Query: 414 VDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCRELVLPNIAKFLPVL 442
                    P  + R  GL+V +   LS    LHLH  +L LP+     P++
Sbjct: 277 ---------PDSVLRALGLTVPQARTLS----LHLHAVQLTLPSSDGSTPIV 292

BLAST of Sed0018704 vs. ExPASy TrEMBL
Match: A0A6J1BS40 (RNA pseudouridine synthase 3, mitochondrial isoform X3 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.3e-254
Identity = 424/473 (89.64%), Postives = 451/473 (95.35%), Query Frame = 0

Query: 10  MRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 69
           M+QS+RR+LL+AARYYSRISPPPPAYAKP+IRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 70  YSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQSSCSDGKGN 129
           Y LP KNHIG+SG STHVTAI WVKHYFDEI D V+ SHFR+GL++VECPQS  S+GKGN
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120

Query: 130 LKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 189
           LKPMRKI  NEIMETGAR+HIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 190 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTE 249
           SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGILLMGRT+
Sbjct: 181 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTK 240

Query: 250 ESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDD 309
           ESVAHLQWLFSNIRN KSSCKAW+EA +ATYQKYWALVIGCP+EREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 310 GKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 369
           GKTERVVLA++TS+E  QEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 370 IVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCREL 429
           IVGDYKYGWFVHR+WKQMPRVDVE ISG+PYKLRRPEGL VQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420

Query: 430 VLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           VLPNIAKF+ VL +KRSI QHP+S  +SDLLRFVA+MPSHMRISWNLMSSYLV
Sbjct: 421 VLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Sed0018704 vs. ExPASy TrEMBL
Match: A0A6J1K8P8 (RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111492130 PE=4 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.6e-251
Identity = 416/473 (87.95%), Postives = 448/473 (94.71%), Query Frame = 0

Query: 10  MRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 69
           MRQS+RR+LL+AARYYSRISPPPPAYA+P+IRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLAARYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 70  YSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQSSCSDGKGN 129
           Y LP KN IG SGESTHVTAI WVKHYFDEI D V+ SHFR+GLV+V+CPQS   DG+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 130 LKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 189
           LKPMRKI  NEIMETGAR+HIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 190 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTE 249
           SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGI+LMGRT+
Sbjct: 181 SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRTK 240

Query: 250 ESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDD 309
           ESVAHLQWLFSNIRN KSSCKAW+EA +ATYQKYWALVIGCP+EREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 310 GKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 369
           GKTERVVLAN+TS E  QEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKTSFEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 370 IVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCREL 429
           IVGDYKYGWFVHR+WKQMPRVD+EPISG+PYKLRRP+GL VQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCREL 420

Query: 430 VLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           VLPN+AKFL VLDDKRSI  H +S ++SDLLRFVA+MPSHMRISWNLMSSYLV
Sbjct: 421 VLPNLAKFLNVLDDKRSISHHRVSNLNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Sed0018704 vs. ExPASy TrEMBL
Match: A0A6J1HAX8 (RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111462419 PE=4 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.8e-251
Identity = 415/473 (87.74%), Postives = 448/473 (94.71%), Query Frame = 0

Query: 10  MRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 69
           MRQS+RR+LL+A RYYSRISPPPPAYA+P+IRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 70  YSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQSSCSDGKGN 129
           Y LP KN IG SGESTHVTAI WVKHYFDEI+D V+ SHFR+GLV+V+CPQS   DG+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIEDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 130 LKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 189
           LKPMRKI  NEIMETGAR+HIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 190 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTE 249
           SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGI+LMGRT+
Sbjct: 181 SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRTK 240

Query: 250 ESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDD 309
           ESVAHLQWLFSNIRN KSSCKAW+EA +ATYQKYWALVIGCP+EREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 310 GKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 369
           GKTERVVLAN+TS+E  QEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 370 IVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCREL 429
           IVGDYKYGWFVHR+WKQMPRVD+EPISG+PYKLRRP+GL VQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCREL 420

Query: 430 VLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           VLPN+AKFL VLDDKRSI  H +S  +SDLLRFVA+MPSHMRISWNLMSSYLV
Sbjct: 421 VLPNLAKFLNVLDDKRSISHHRVSYFNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Sed0018704 vs. ExPASy TrEMBL
Match: A0A0A0LQ04 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G402020 PE=4 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.8e-242
Identity = 411/476 (86.34%), Postives = 434/476 (91.18%), Query Frame = 0

Query: 7   YGIMRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPP 66
           Y IM++S+RR+LL+A R YSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPK RQLLSLPP
Sbjct: 70  YKIMQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPP 129

Query: 67  FPGYSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQSSCSDG 126
           FPGY LPGKN +GVSGESTHVTAI+WVK+YFDEI D  + SHFR+GLV+VECPQ    D 
Sbjct: 130 FPGYPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDE 189

Query: 127 KGNLKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVK 186
           KGNLKPMRKI   EIMETGARIH+PVSVAEMK++RRFDTIPSGTLYPNADEIKYLQRLVK
Sbjct: 190 KGNLKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVK 249

Query: 187 YKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMG 246
           YKDSAIIVLNKPPKLPVKG LPVHNSMDALA AALSYDYDDGPKLVHRLDRESSGILLMG
Sbjct: 250 YKDSAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMG 309

Query: 247 RTEESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVL 306
           RT+ESV HLQWLFSNIRN K +CKAW EA +ATYQKYWALVIGCP+EREG+ISAPLSKVL
Sbjct: 310 RTKESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVL 369

Query: 307 LDDGKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEAL 366
           LDDGKTERVVLAN+ S+E  QEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEAL
Sbjct: 370 LDDGKTERVVLANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEAL 429

Query: 367 GTPIVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHC 426
           GTPIVGDYKYGWFVHR WKQMPRVDVEPISGRPYKLRRPEGL VQKGSVLSKVPLLHLHC
Sbjct: 430 GTPIVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHC 489

Query: 427 RELVLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           RELVLPNIAKFL V   K      P+S  DSDLLRFVA+MPSHMRISWNLMSSYLV
Sbjct: 490 RELVLPNIAKFLNVFYGKGP--NRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 543

BLAST of Sed0018704 vs. ExPASy TrEMBL
Match: A0A1S3B3J4 (RNA pseudouridine synthase 3, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485411 PE=4 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 5.4e-241
Identity = 409/473 (86.47%), Postives = 431/473 (91.12%), Query Frame = 0

Query: 10  MRQSLRRYLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 69
           M++S+RR+LL+A RYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPK RQLLSLPPFPG
Sbjct: 1   MQRSIRRHLLLATRYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60

Query: 70  YSLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVECPQSSCSDGKGN 129
           + LPGKN +GVSGESTHVTAI+WVK+YFDEI D  + S FR+GLV+VECPQ    D  GN
Sbjct: 61  HPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSLFRKGLVQVECPQLGGFDENGN 120

Query: 130 LKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 189
           LKPMRKI   EIME GARIH+PVSVAEMK+SRRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSGEIMEIGARIHLPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 190 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTE 249
           SAIIVLNKPPKLPVKG LPVHNSMDALAAAALSYDYD GPKLVHRLDRESSGILLMGRT+
Sbjct: 181 SAIIVLNKPPKLPVKGTLPVHNSMDALAAAALSYDYDYGPKLVHRLDRESSGILLMGRTK 240

Query: 250 ESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDD 309
           ESV HLQWLFSNIRNGK +CKAW EA +ATYQKYWALVIG P+EREGLISAPLSKVLLDD
Sbjct: 241 ESVDHLQWLFSNIRNGKFTCKAWEEACNATYQKYWALVIGRPREREGLISAPLSKVLLDD 300

Query: 310 GKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 369
           GKTERVVLAN+ S+E  QEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKASLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 370 IVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCREL 429
           IVGDYKYGWFVHR WKQMPRVDVEPISGRPYKLRRPEGL VQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420

Query: 430 VLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           VLPNIAKFL V  DK  I   P+S  D DLLRFVA+MPSHMRISWNLMSSYLV
Sbjct: 421 VLPNIAKFLNVFYDKGPISHRPVSSFDFDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Sed0018704 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 666.0 bits (1717), Expect = 2.3e-191
Identity = 321/472 (68.01%), Postives = 387/472 (81.99%), Query Frame = 0

Query: 17  YLLVAARYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYSLPGKN 76
           YL V  R YSR++PPP +    +IRV+NN+A LG PK+GP PRQL+SLPPFPG+ LPGKN
Sbjct: 12  YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71

Query: 77  HIGVSGE----STHVTAIKWVKHYFDEIDDLVVHSHFREGLVRVEC--PQSSCSDGKGNL 136
             G  G+      HVTAI WVK+YF+EI D  + +HF +GLV++E    + +  + +   
Sbjct: 72  -AGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDGA 131

Query: 137 KPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKDS 196
            PMRKI  NE+M+ G +I +PVS+AEM+IS+R+DTIPSGTLYPNADEI YLQRLV++KDS
Sbjct: 132 IPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKDS 191

Query: 197 AIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTEE 256
           AIIVLNKPPKLPVKGN+P+HNSMDALAAAALS+  D+GP+LVHRLDRE+SG+L+MGRT+E
Sbjct: 192 AIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRTKE 251

Query: 257 SVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDDG 316
           S+ +L  +FS+ +   SSCKAWN+A +A YQ+YWALVIG PKE+EGLISAPLSKVLLDDG
Sbjct: 252 SIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLDDG 311

Query: 317 KTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPI 376
           KT+RVVLA  +  E  Q+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGTPI
Sbjct: 312 KTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGTPI 371

Query: 377 VGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQKGSVLSKVPLLHLHCRELV 436
           VGDYKYGWFVH+ WKQMP+VD+EP +G+PYKLRRPEGL VQKGSVLSKVPLLHLHCRE+V
Sbjct: 372 VGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREMV 431

Query: 437 LPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRISWNLMSSYLV 483
           LPNIAKFL V++ + +   H       DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 LPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

BLAST of Sed0018704 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 276.2 bits (705), Expect = 5.1e-74
Identity = 161/427 (37.70%), Postives = 241/427 (56.44%), Query Frame = 0

Query: 62  LSLPPFPGY---------SLPGKNHIGVSGESTHVTAIKWVKHYFDEIDDLVVHSHFREG 121
           L+LPPF             L   + +  S +++  TA++W+     ++   +V   FR  
Sbjct: 56  LTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFRLR 115

Query: 122 LVRVECPQSSCSDGKGNLKPMRKIWGNEIMETGARIHIPVSVAEMKISRRFDTIPSGTLY 181
            VR E   S   D +     ++++   E +  G RI++P+SV     + +       +  
Sbjct: 116 QVRREMSMSVDGD-ELQRSQLKRVAAKESLNVGDRIYLPLSVD--NDTPQTPPAKKESFQ 175

Query: 182 PNADEIKYLQRLVKYKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLV 241
            + +E K++  LV YKD AIIVLNKP  L V+G   +  S+D LAA+ L +D  + P+LV
Sbjct: 176 CSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLV 235

Query: 242 HRLDRESSGILLMGRTEESVAHLQWLFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPK 301
           HRLDR+ SG+L++ RT+ +   L  +F     G S+         +  +KY ALVIGCP 
Sbjct: 236 HRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGVKKNVK-SLKRKYMALVIGCPP 295

Query: 302 EREGLISAPLSKVLLDDGKTERVVLANRTSMEPYQEAITEYRVLGPTINGCSWIELCPRT 361
            + G ISAPL KV++DDGK+ER+ + +   +   Q AITEYRV+  + +G +W+EL P T
Sbjct: 296 RQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLT 355

Query: 362 SRKHQLRIHCAEALGTPIVGDYKYGWFVHRSWKQMPRVDVEPISGRPYKLRRPEGLSVQK 421
            RKHQLR+HCAE LGTPIVGDYKYGW  H++ +     +  P      +   P GL +  
Sbjct: 356 GRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSSENNPTK----QSSSPFGLDLDG 415

Query: 422 GSVLSKVPLLHLHCRELVLPNIAKFLPVLDDKRSIGQHPISRVDSDLLRFVAKMPSHMRI 480
           G V SK P LHLH +++ LPNI++   +L+  +      IS +DS  L+F A +PSHM++
Sbjct: 416 GDVSSKQPHLHLHSKQIDLPNISQ---LLEKMQVSSDSDISDLDS--LKFDAPLPSHMQL 469

BLAST of Sed0018704 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 61.2 bits (147), Expect = 2.6e-09
Identity = 56/241 (23.24%), Postives = 93/241 (38.59%), Query Frame = 0

Query: 171 LYPNADEIKYLQRLVKYKDSAIIVLNKPPKL---PVKGN--------------LP----- 230
           L P   E + +   + Y+D  ++V+NKP  +   P  GN              LP     
Sbjct: 136 LQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYS 195

Query: 231 -----------VHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTEESVAHLQW 290
                        +  + +  +  SY     P +VHRLD+ ++G+L++ + E S AHL  
Sbjct: 196 NSEEDDDSDEETFSDDEEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAE 255

Query: 291 LFSNIRNGKSSCKAWNEASDATYQKYWALVIGCPKEREGLISAPLSKVLLDDGKTERVVL 350
            F                     + Y +L  G P   +G I  P+ +      +     +
Sbjct: 256 QFK---------------LHTIERVYVSLTTGVPSPPQGRIEIPIGRD--SSNRIRMAAI 315

Query: 351 ANRTSMEPYQEAITEYRVLGPTINGCS-WIELCPRTSRKHQLRIHCAEALGTPIVGDYKY 378
                    + A + Y+V+     G S  +E    T R HQ+R H A+ +G P++GD  Y
Sbjct: 316 PGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDEVY 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131827.18.8e-25489.64RNA pseudouridine synthase 3, mitochondrial isoform X3 [Momordica charantia][more]
XP_022997125.15.4e-25187.95RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima] >XP_022997126.1 R... [more]
XP_022961737.11.2e-25087.74RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata] >XP_022961738.1... [more]
XP_038885249.12.3e-24986.51LOW QUALITY PROTEIN: RNA pseudouridine synthase 3, mitochondrial [Benincasa hisp... [more]
XP_023545207.11.5e-24887.10RNA pseudouridine synthase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023... [more]
Match NameE-valueIdentityDescription
Q5XET63.2e-19068.01RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q0J4D47.5e-17161.97RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q9LT727.2e-7337.70RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q69K071.1e-4435.20RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q28C597.8e-2731.85Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1BS404.3e-25489.64RNA pseudouridine synthase 3, mitochondrial isoform X3 OS=Momordica charantia OX... [more]
A0A6J1K8P82.6e-25187.95RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1HAX85.8e-25187.74RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A0A0LQ043.8e-24286.34PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G40... [more]
A0A1S3B3J45.4e-24186.47RNA pseudouridine synthase 3, mitochondrial isoform X1 OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
AT1G78910.12.3e-19168.01Pseudouridine synthase family protein [more]
AT3G19440.15.1e-7437.70Pseudouridine synthase family protein [more]
AT1G76050.22.6e-0923.24Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 192..363
e-value: 7.5E-23
score: 81.4
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 192..388
e-value: 6.8266E-42
score: 145.558
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 178..437
e-value: 5.2E-47
score: 162.2
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 15..479
NoneNo IPR availablePANTHERPTHR21600:SF53RNA PSEUDOURIDINE SYNTHASE 3, MITOCHONDRIALcoord: 15..479
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 178..433

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0018704.1Sed0018704.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding