Sed0017959 (gene) Chayote v1

Overview


Name	Sed0017959
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Auxin-responsive GH3 family protein
Location	LG01: 5063623 .. 5067229 (+)
RNA-Seq Expression	Sed0017959
Synteny	Sed0017959

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCATGATGCACAAAACCTGCTAGAGGAGAAAGATAAGCAAACGTTGCAACATCTTGAACATATAACGAGCAACGCCGACGAGATCCAACGACAAACTCTGGTCGAAATTTTGTCGACAAATGCTGATGTTGAGTACTTGCAGCAACACGGCCTTAACGGATCTACTGATGCTTCAACTTTCAAGAACCTTATCCCTCTCATATCTTACGAACAATTAAAACCTTACGTAGAACGGATTGCTAATGGTGATGATTCTCCAATCCTATGCGCTGAACCCATTACAGAGTTTTTTATCAGGTGACTCTTTTCTCTACTTTAAATTGCATGCCATCATCAAGCATAATATTTCCCGATTAAAATAATATATAAATATATATTTGACCAAATAAGAAAGGAAAAGTTGACTAAAAAGAATAAAAATAACTGAAAAGTAAATAAAAGTTGATTTTTAAAAATAAAATCAATAAGATGACAAGGGTAAGAATCAAGCCATGTGGAAAAGACAATTGATTTTGACAGCTTACAACAGCTGCCTCTATGCCAGTCTTAAATTATGATCACAAAGAACTTAATATTATAAGGACATAAACAAAGCAACATTAAAATGACATGTTTGGCCTTCTTACTTATCAATGAGAAAGAAAGAGAAATTTTATGTCAGTGTTTGGCCACCTGGGTTATTTCTTCCCAGCTGGGAAGGGAAGGTATAACCCACCCTTTTTTTTTGTTTTTGGTCAATTTTTGTTTTTTAGTCTATTTTCTTACTTTTTAAGTTTTTATACACAGAGTTTATTACCTCCATTTTTTTGTATCTAATCAAATTTATTTTGTATATATATATATTTTTTGAATTTTGCCTTCAACTTTTTAGTAATGCTTATCAATATAAATTTATGATAATTTGATTGTTTTTATCTGATTATCAAATTTAATTATTGGATAATAATAACGATCTAAATATATAAAAATTAAATTTTCATTAAGTAATAGTTAAAATAAAATGAGTCATCTTTTAAATTGAAACTGAGTGTTTATTTTAATTTTTGTAATGTTTTTATAATTATATTTTGGTATATTATTATTCAAATTAAATAGTCAAAATTAAGTTTGGAACCTTCGTTTTCCTTAAATCAAGAATTAAAGTTGTTAACTTTGAACCATTAATTAATTTAATTTAATTGATTATATTTATTTATTCTGTGTATATTTATTTATTTTAAAGTGTAATATTTATCATTTCTTTTTAAAATATATATGGTTCTTTAAATAATATTTGTTAGTGTTTATACATTCGATTTTTATCATGCATTTGAAATTTGTTGTATAAAAATATATATTAATAAATATTTATCGTATTATTTATCAATTTTTTCTAACTCGTGCATGTTTTTTAATAAATATATGAAAATACATATCCATATGATTTAAAGATATAAACACTTGAAATAATTAAACTAATATATTTTTCTTTTGGTGTAACTCATTTTGATTTGAATTTTTAAACTTAATTTTAAATTTTATTTAAATAATAAATAAAATAAATTTCAAAAATGATATTTTATGAATATTACATTAAAAAAAAATATCTATTATTTAAAAAAAAATTACATCACTTCCTTTCCCTTCCCACCAAACATAAATTTATTATTACATCACTTTTTTCCCTTTCCACCAAACACTAATTTACTCCAACTTCCCTTTCTTTCCTTTCCCTTCCCGCCCTAGCCAAACATGCCCAAAATGAGATACTAGAAACTTCATTTGAGAGTTTTTCATTTTTTAGTTAAATCTTAGAAACGTTTCTATTAATTTTGGAAACAAGAAATGTAAGTAGTTACCAAACAAGTTTGTTTCTCAAAAAAGAAGAAACATAAACGAGAAACGGGAACGTTACCAAACGGGCCCTTAAATGCTTAATTTTTTAGAAAACTATCTTCTTAATCCCTGGGATTGGACTAAAACATTGCATTATTAACATTTATGGAGAAAAGTGTTTGATTTTGAATCATAGCTACCAAATGTAGAAATAACCTCTTAAACTTAAGGATTAAAAATCTACGTTTTCCTTAATTTTTTGATGGGGGATTTGATTAACCTCAAAGACACTTTATTTTTCTTGTAGCTCTGGAACTTCAGGGGGAGAACAAAAATTAATTCCAATATTTGAACGAGAGATTTGGAGAAGACTTTCTTTTTTTAGCTATGTGTTCGCCAAAATGAAACAGTTGTTTCCAAATGTGCACAAAGGAAAAGAAATGTTCTTTCGCTTTAACAAGACTGGGTCAAAAACCAAAGGTGGAATTGTTGCTAATTCTGTATCCACAAGTTTCTGCAACAGAACCCACTTATTCAAAAATTCTCAAAGTGGAGGTGACACAAGTCCTAACAATGTCTTTTTGTGCACAGACTTGTACCAAAGCACATATTCTCAGTTGCTTTGTGGGCTTTGTGAAAATGAGGAAGTTCTTAAGTTGGGTGCTCTATTTGCCTCTACTTTAGTTTCTATTTTGAAGTTCCTCGAAAATCATTGGGTTCTTTTAGCTAATGATATACGAACAGGAACAATAAATCCCAAAATTACAGATTCCTCGGTGAGAGAATCGATTATGAACATTCTAAGACCCAATCCCGAGCTTGCAGACTTCATTGAAACAGAATGTAGCAAAGGAACATGGGAAGGAATCATCACGAGATTATGGCCAAACACAAAATACATTAAAGCTATTGTGACAGGAAGCATGTCACAATACATTCCCCTACTTGATTACTATAGTAATAATCTCCCTCTTATTTCTGAGTACTATAGTTCTTCGGAATGCTTTTTTGGTTTAAACTTAGATCCATTGTGCAAACCAGAAAAAATTTCCTACACTTTAATCCCCAATATGGCATATTTTGAGTTCTTGCCAGTTGAGAGAGGAAAAGATGATAGCAAAGCAGACAAGGAATTAGTGGATCTCGTGGATGTGAAGTTGGGACAAGAATATGAGCTCGTCATCACCACTTATGCTGGCCTCTACCGTTATCGCATGGGTGATATTCTACGTGTAACAGGGTTCAAAAATAAAGTTCCAAAGTTAAGTTTTGTATGCAAGAAAGACGTTGTACTAAGCCTTGATATTGAAAAGACAGATGAAGCTGAGCTTTATAATGGCATACAAGAAGCTGCTAAGCTTTTAAGCCCATTTGGGGCAAATATTGCTGATTACACAAGCTATGCAGATAGCTCAACCGTTCCTGGACATTATGTATTGTATTGGGAAATTCTTTGTAAGGAACAAAGCTCAACGATTCCATCTTCAATTTACGAGGATTGTTGCTTGGCTGTTGAAGAGTCGCTTAATGTATTTTATCGTAGAGGAAGATCTCAAGAAAAAAATATCAATCCACTAGAGATAAAGATTTTAGAGGATGGAACATTTGAAAAACTCATGGAGCGAGCACTGGATCGAGGAGCATCGATGGCTCAATACAAGACCCCTAGATGCATAAATTCTATGCATATAATTGAGTTTTTGGACTCAAGAATTTTGTCCAACCATTTTAGCCAAAAATGTCCAAAATGGGATCCTAGTCGTCGACATCGTCTTTTTTCCTCCTAG

mRNA sequence

ATGTCTCATGATGCACAAAACCTGCTAGAGGAGAAAGATAAGCAAACGTTGCAACATCTTGAACATATAACGAGCAACGCCGACGAGATCCAACGACAAACTCTGGTCGAAATTTTGTCGACAAATGCTGATGTTGAGTACTTGCAGCAACACGGCCTTAACGGATCTACTGATGCTTCAACTTTCAAGAACCTTATCCCTCTCATATCTTACGAACAATTAAAACCTTACGTAGAACGGATTGCTAATGGTGATGATTCTCCAATCCTATGCGCTGAACCCATTACAGAGTTTTTTATCAGCTCTGGAACTTCAGGGGGAGAACAAAAATTAATTCCAATATTTGAACGAGAGATTTGGAGAAGACTTTCTTTTTTTAGCTATGTGTTCGCCAAAATGAAACAGTTGTTTCCAAATGTGCACAAAGGAAAAGAAATGTTCTTTCGCTTTAACAAGACTGGGTCAAAAACCAAAGGTGGAATTGTTGCTAATTCTGTATCCACAAGTTTCTGCAACAGAACCCACTTATTCAAAAATTCTCAAAGTGGAGGTGACACAAGTCCTAACAATGTCTTTTTGTGCACAGACTTGTACCAAAGCACATATTCTCAGTTGCTTTGTGGGCTTTGTGAAAATGAGGAAGTTCTTAAGTTGGGTGCTCTATTTGCCTCTACTTTAGTTTCTATTTTGAAGTTCCTCGAAAATCATTGGGTTCTTTTAGCTAATGATATACGAACAGGAACAATAAATCCCAAAATTACAGATTCCTCGGTGAGAGAATCGATTATGAACATTCTAAGACCCAATCCCGAGCTTGCAGACTTCATTGAAACAGAATGTAGCAAAGGAACATGGGAAGGAATCATCACGAGATTATGGCCAAACACAAAATACATTAAAGCTATTGTGACAGGAAGCATGTCACAATACATTCCCCTACTTGATTACTATAGTAATAATCTCCCTCTTATTTCTGAGTACTATAGTTCTTCGGAATGCTTTTTTGGTTTAAACTTAGATCCATTGTGCAAACCAGAAAAAATTTCCTACACTTTAATCCCCAATATGGCATATTTTGAGTTCTTGCCAGTTGAGAGAGGAAAAGATGATAGCAAAGCAGACAAGGAATTAGTGGATCTCGTGGATGTGAAGTTGGGACAAGAATATGAGCTCGTCATCACCACTTATGCTGGGTTCAAAAATAAAGTTCCAAAGTTAAGTTTTGTATGCAAGAAAGACGTTGTACTAAGCCTTGATATTGAAAAGACAGATGAAGCTGAGCTTTATAATGGCATACAAGAAGCTGCTAAGCTTTTAAGCCCATTTGGGGCAAATATTGCTGATTACACAAGCTATGCAGATAGCTCAACCGTTCCTGGACATTATGTATTGTATTGGGAAATTCTTTGTAAGGAACAAAGCTCAACGATTCCATCTTCAATTTACGAGGATTGTTGCTTGGCTGTTGAAGAGTCGCTTAATGTATTTTATCGTAGAGGAAGATCTCAAGAAAAAAATATCAATCCACTAGAGATAAAGATTTTAGAGGATGGAACATTTGAAAAACTCATGGAGCGAGCACTGGATCGAGGAGCATCGATGGCTCAATACAAGACCCCTAGATGCATAAATTCTATGCATATAATTGAGTTTTTGGACTCAAGAATTTTGTCCAACCATTTTAGCCAAAAATGTCCAAAATGGGATCCTAGTCGTCGACATCGTCTTTTTTCCTCCTAG

Coding sequence (CDS)

ATGTCTCATGATGCACAAAACCTGCTAGAGGAGAAAGATAAGCAAACGTTGCAACATCTTGAACATATAACGAGCAACGCCGACGAGATCCAACGACAAACTCTGGTCGAAATTTTGTCGACAAATGCTGATGTTGAGTACTTGCAGCAACACGGCCTTAACGGATCTACTGATGCTTCAACTTTCAAGAACCTTATCCCTCTCATATCTTACGAACAATTAAAACCTTACGTAGAACGGATTGCTAATGGTGATGATTCTCCAATCCTATGCGCTGAACCCATTACAGAGTTTTTTATCAGCTCTGGAACTTCAGGGGGAGAACAAAAATTAATTCCAATATTTGAACGAGAGATTTGGAGAAGACTTTCTTTTTTTAGCTATGTGTTCGCCAAAATGAAACAGTTGTTTCCAAATGTGCACAAAGGAAAAGAAATGTTCTTTCGCTTTAACAAGACTGGGTCAAAAACCAAAGGTGGAATTGTTGCTAATTCTGTATCCACAAGTTTCTGCAACAGAACCCACTTATTCAAAAATTCTCAAAGTGGAGGTGACACAAGTCCTAACAATGTCTTTTTGTGCACAGACTTGTACCAAAGCACATATTCTCAGTTGCTTTGTGGGCTTTGTGAAAATGAGGAAGTTCTTAAGTTGGGTGCTCTATTTGCCTCTACTTTAGTTTCTATTTTGAAGTTCCTCGAAAATCATTGGGTTCTTTTAGCTAATGATATACGAACAGGAACAATAAATCCCAAAATTACAGATTCCTCGGTGAGAGAATCGATTATGAACATTCTAAGACCCAATCCCGAGCTTGCAGACTTCATTGAAACAGAATGTAGCAAAGGAACATGGGAAGGAATCATCACGAGATTATGGCCAAACACAAAATACATTAAAGCTATTGTGACAGGAAGCATGTCACAATACATTCCCCTACTTGATTACTATAGTAATAATCTCCCTCTTATTTCTGAGTACTATAGTTCTTCGGAATGCTTTTTTGGTTTAAACTTAGATCCATTGTGCAAACCAGAAAAAATTTCCTACACTTTAATCCCCAATATGGCATATTTTGAGTTCTTGCCAGTTGAGAGAGGAAAAGATGATAGCAAAGCAGACAAGGAATTAGTGGATCTCGTGGATGTGAAGTTGGGACAAGAATATGAGCTCGTCATCACCACTTATGCTGGGTTCAAAAATAAAGTTCCAAAGTTAAGTTTTGTATGCAAGAAAGACGTTGTACTAAGCCTTGATATTGAAAAGACAGATGAAGCTGAGCTTTATAATGGCATACAAGAAGCTGCTAAGCTTTTAAGCCCATTTGGGGCAAATATTGCTGATTACACAAGCTATGCAGATAGCTCAACCGTTCCTGGACATTATGTATTGTATTGGGAAATTCTTTGTAAGGAACAAAGCTCAACGATTCCATCTTCAATTTACGAGGATTGTTGCTTGGCTGTTGAAGAGTCGCTTAATGTATTTTATCGTAGAGGAAGATCTCAAGAAAAAAATATCAATCCACTAGAGATAAAGATTTTAGAGGATGGAACATTTGAAAAACTCATGGAGCGAGCACTGGATCGAGGAGCATCGATGGCTCAATACAAGACCCCTAGATGCATAAATTCTATGCATATAATTGAGTTTTTGGACTCAAGAATTTTGTCCAACCATTTTAGCCAAAAATGTCCAAAATGGGATCCTAGTCGTCGACATCGTCTTTTTTCCTCCTAG

Protein sequence

MSHDAQNLLEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKNLIPLISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSYVFAKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQSGGDTSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIRTGTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTGSMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVERGKDDSKADKELVDLVDVKLGQEYELVITTYAGFKNKVPKLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVLYWEILCKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMERALDRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRRHRLFSS

Homology

[Close]

BLAST of Sed0017959 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 819.3 bits (2115), Expect = 2.1e-233
Identity = 394/580 (67.93%), Postives = 468/580 (80.69%), Query Frame = 0


Query: 9   LEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKNLIPL 68
           L+EKDKQ LQ LE +T  ADEIQR+ L EILSTNA VEYLQQHGLNGSTDASTFKNLIP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  ISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSY 128
           +SYE LKPY++RI NGDDSPILCA PITEF+ S+GTSGGEQKLIP  E+E  R+LS  +Y
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 VFAKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQSGGDTSP 188
           +  +MKQLFP +H GKEMFF F K  S TK GIVA SVSTSF    HL         TSP
Sbjct: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183

Query: 189 NNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIRTGT 248
            ++  CTD+YQS Y QLLCGLC+N++V+++G+LFASTL+ I KFLENHWVLLANDIRTGT
Sbjct: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243

Query: 249 INPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTGSMS 308
           IN KI DS VR+S+MNIL+P+P+LADFIE+ECSKGTWEGI+TRLWPNTK+I A+VTGSMS
Sbjct: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303

Query: 309 QYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVERGK 368
            YIP L+YY  +LPLI+ +Y+SSECFFG+NLDPLCKPE+ISYT IP +AYFEFLPV+R  
Sbjct: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363

Query: 369 DDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVPKLSFVCKKDV 428
           D  +  +E VDLVDVKLGQEYE+V+TTYA              GFKNK P   FV +K+V
Sbjct: 364 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 423

Query: 429 VLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVLYWEILCKEQS 488
           +LSLD+EKTDEAEL+N + +   LLS FGA + DYTSYADSST PGHYVLYWEI  KE+ 
Sbjct: 424 ILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEI 483

Query: 489 STIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMERALDRGASM 548
           S IPSSIYEDCCLA+EESL+  YRR RS +K INPLEIKI+E  TF+KLMER +D+GAS+
Sbjct: 484 SIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 543

Query: 549 AQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRRH 575
           +QYKTPRC+ S  IIEFLDS++++N+FS+K PKWDP+R H
Sbjct: 544 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583

[Close]

BLAST of Sed0017959 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 808.9 bits (2088), Expect = 2.8e-230
Identity = 401/594 (67.51%), Postives = 474/594 (79.80%), Query Frame = 0


Query: 1   MSHDAQNLLEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDAS 60
           M+H  +   +E+DKQ LQH+E +T +A +IQRQ L +ILSTNADVEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLIPLISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIW 120
           TFKNLIPLISYEQL+PY++RIANGDDSPILC  PITEFFISSGTSGGE KLIP++E E  
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLSFFSYVFAKMKQLFPN--VHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFK 180
           RRLSFF YV  +MKQLFPN  V KGK M F+F K   KT+ GIVA++ +TS C R +L  
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANL-- 180

Query: 181 NSQSGGDTSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWV 240
                 +TSP ++ LCTD  QS Y QLLCGLC+N+ V ++GALFASTL+ +LKFLE+HWV
Sbjct: 181 -----NNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWV 240

Query: 241 LLANDIRTGTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKY 300
           +LA+DIR GT+N KITD SVRE++MNIL PN +LADFIE ECSKGTWEGIITRLWPN KY
Sbjct: 241 VLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKY 300

Query: 301 IKAIVTGSMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAY 360
           I AIVTGSM+QY+P L+YYSN+LP++S++Y +SECF GLNLDPLC P++ SYTLIP MAY
Sbjct: 301 INAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAY 360

Query: 361 FEFLPVERGKDDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVP 420
           FEFLP++R K   +  +ELVDLVDVKLG EYELVITTYA              GFKNK P
Sbjct: 361 FEFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAP 420

Query: 421 KLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVL 480
             SFVC+K+VVLSLDIEKTDEAEL++ +QEAA+LLSPFGA IADYTS+ADSS +PGHYVL
Sbjct: 421 NFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVL 480

Query: 481 YWEILCKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLM 540
           YWEI  K   S IPSSIYEDCC A+EE LN  YR  RS EK INPLEIKI++ GTFEKLM
Sbjct: 481 YWEIHVKGGIS-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLM 540

Query: 541 ERALDRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRRHRLFS 579
            +AL+RGASM+QYKTPRC+NS HIIE LDS ++  +FS+KCPK D    HRL S
Sbjct: 541 AQALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

[Close]

BLAST of Sed0017959 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 808.5 bits (2087), Expect = 3.7e-230
Identity = 400/594 (67.34%), Postives = 475/594 (79.97%), Query Frame = 0


Query: 1   MSHDAQNLLEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDAS 60
           M+H  +   +E+DKQ LQH+E +T +A +IQRQ L +ILSTNADVEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLIPLISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIW 120
           TFKNLIPLISYEQL+PY++RIANGDDSPILC  PITEFFISSGTSGGE KLIP++E E  
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLSFFSYVFAKMKQLFPN--VHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFK 180
           RRLSFF YV  +MKQLFPN  V KGK M F+F K   KT+ GIVA++ +TS C R +L  
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANL-- 180

Query: 181 NSQSGGDTSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWV 240
                 +TSP ++ LCTD  QS Y QLLCGLC+N+ V ++GALFASTL+ +LKFLE+HWV
Sbjct: 181 -----NNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWV 240

Query: 241 LLANDIRTGTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKY 300
           +LA+DIR GT+N KITD SVRE++MNIL PN +LADFIE ECSKGTWEGIITRLWPN KY
Sbjct: 241 VLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKY 300

Query: 301 IKAIVTGSMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAY 360
           I AIVTGSM+QY+P L+YYSN+LP++S++Y  SECF GLNLDPLC P++ SYTLIP MAY
Sbjct: 301 INAIVTGSMAQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMAY 360

Query: 361 FEFLPVERGKDDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVP 420
           FEFLP++R K   +  +ELVDLVDVKLG EYELVITTYA              GFKNK P
Sbjct: 361 FEFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYCMGDILRVTGFKNKAP 420

Query: 421 KLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVL 480
             SFVC+K+VVLSLDIEKTDEAEL++ +QEAA+LLSPFGA IADYTS+ADSS +PGHYVL
Sbjct: 421 NFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVL 480

Query: 481 YWEILCKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLM 540
           YWEI  K   S IPSSIYEDCC A+EE LN  YR  RS EK INPLEIKI++ GTFEKLM
Sbjct: 481 YWEIHVKGGIS-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLM 540

Query: 541 ERALDRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRRHRLFS 579
            +AL+RGASM+QYKTPRC+NS HIIE LDS +++ +FS++CPK D +  HRL S
Sbjct: 541 AQALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKVDTNALHRLLS 586

[Close]

BLAST of Sed0017959 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 771.2 bits (1990), Expect = 6.5e-219
Identity = 382/596 (64.09%), Postives = 469/596 (78.69%), Query Frame = 0


Query: 1   MSHDAQNLLEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDAS 60
           MSH  ++ L+EKDK+ LQH+E +TS ADEIQRQ L EILSTNA+VEYLQQHGL+GSTDAS
Sbjct: 1   MSHVVEHWLDEKDKEALQHIEDVTSKADEIQRQILTEILSTNANVEYLQQHGLHGSTDAS 60

Query: 61  TFKNLIPLISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIW 120
           TFK LIPLISYEQL PY+  IA+ DDSPILC+ PIT FF SSGTSGGE KLIP++E E  
Sbjct: 61  TFKKLIPLISYEQLTPYLALIAHDDDSPILCSNPITHFFTSSGTSGGETKLIPMYEEEFV 120

Query: 121 RRLSFFSYVFAKMKQLFPNVH--KGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFK 180
           RRLSFF+Y+  +MKQLFPN++  KGK M F F K   KTK GI+  S+ TS   R+   K
Sbjct: 121 RRLSFFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTKAGIIVRSIFTSLHKRSSNLK 180

Query: 181 NSQSGGDTSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWV 240
           +   G +TSP+++ LCTD +QS Y QLLCGL +N+ V +LGALFASTL+ + KFLENHWV
Sbjct: 181 SKPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRLGALFASTLIHVFKFLENHWV 240

Query: 241 LLANDIRTGTINPKITDSSVRESIMNI-LRPNPELADFIETECSKGTWEGIITRLWPNTK 300
           +LANDI TG+INPKIT+ SVRES+M I ++PNPE+AD IE ECSKG+WEGII RLWPNTK
Sbjct: 241 VLANDILTGSINPKITNLSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300

Query: 301 YIKAIVTGSMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMA 360
           YI AIVTGSMSQYIPLL++Y+NNLP++S++Y SSECF GLNLDPLC P +ISYTLIP MA
Sbjct: 301 YINAIVTGSMSQYIPLLNFYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 360

Query: 361 YFEFLPVERGKDDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKV 420
           YFEFLP++R   + +  ++LVDLVDVKLGQEYELVITT+A              GF  K 
Sbjct: 361 YFEFLPIDRTNPNGEVTQQLVDLVDVKLGQEYELVITTFAGLYRYRMGDIIRVTGFTKKA 420

Query: 421 PKLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYV 480
           P  SFVC+K+VVLSL+ EKTDEA L+  ++E   +L PFGA I DYTSYADSST+PGHYV
Sbjct: 421 PNFSFVCRKNVVLSLNNEKTDEAGLHKAVEEGGHVLKPFGATIVDYTSYADSSTIPGHYV 480

Query: 481 LYWEILCKEQ-----SSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDG 540
           LY+E++  E      S++IPSS+YEDCC A+EE+LN  YR  RS EK I+PLEIKI++ G
Sbjct: 481 LYFELIFDEHNKAQISNSIPSSVYEDCCFAIEETLNSLYRLARSHEKCIDPLEIKIVKAG 540

Query: 541 TFEKLMERALDRGASMAQYKTPRCINS--MHIIEFLDSRILSNHFSQKCPKWDPSR 573
           TFEKLM+ A++RGAS+ +YKTPRC+NS  +HII+ L+S ++SN+FSQK PK D +R
Sbjct: 541 TFEKLMQLAINRGASITRYKTPRCLNSKQIHIIQLLESNLVSNYFSQKSPKLDANR 596

[Close]

BLAST of Sed0017959 vs. NCBI nr
Match: XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])

HSP 1 Score: 765.0 bits (1974), Expect = 4.7e-217
Identity = 373/579 (64.42%), Postives = 463/579 (79.97%), Query Frame = 0


Query: 5   AQNLLEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKN 64
           + ++L+ KDKQ L H+E  TSNADEIQR+ + EILS NA VEYL+Q GL+GSTD STFK 
Sbjct: 2   SDDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKK 61

Query: 65  LIPLISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLS 124
           LIP++SYEQLKPY++RIA+GDDSPILCA+ IT+F IS+GTSGG QKLIP+F+ E+ RRL+
Sbjct: 62  LIPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLA 121

Query: 125 FFSYVFAKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQ-SG 184
              Y    MKQLFP +  GKEMFF F +  +KTKGG+VA  +S S  ++  LF + +   
Sbjct: 122 TNYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLS-SCLSKCSLFSSVEVLR 181

Query: 185 GDTSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLAND 244
             TSP ++  CTD+YQS Y QLLCGLC+N+ VL++GALFASTL+ I KFLENHW LL ND
Sbjct: 182 AYTSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVND 241

Query: 245 IRTGTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIV 304
           IRTGT++ KI D  VRES+M++L+PNPELADFIE+ECSKG WEGI+TRLWPNTKYI  +V
Sbjct: 242 IRTGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVV 301

Query: 305 TGSMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLP 364
           TGSMSQYIP L++Y    P++S +Y+SSECFFG+NLDPLCKPE+ISYT +P MAYFEFLP
Sbjct: 302 TGSMSQYIPQLNFYC-KCPIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLP 361

Query: 365 VER-GKDDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVPKLSF 424
           V+R   ++ +  +ELVDLVDVKLGQEYE+V+TTYA              GFKNK P  SF
Sbjct: 362 VDRANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSF 421

Query: 425 VCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVLYWEI 484
            C+K+V+LSLDIEKTDE +L++ + EA+ LLSP GA I DYTSYADSST PGHYVLYWEI
Sbjct: 422 ACRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEI 481

Query: 485 LCKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMERAL 544
            C+E++STIPS++YEDCCLAVEESL+  YR+ RS  K INPLEIKI+E GTFEKLME+A+
Sbjct: 482 QCREETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAV 541

Query: 545 DRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPK 568
           DRGAS++QYKTPRC+ S  +IE LDS+++SN+FSQ+ PK
Sbjct: 542 DRGASISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

[Close]

BLAST of Sed0017959 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 2.3e-198
Identity = 334/592 (56.42%), Postives = 434/592 (73.31%), Query Frame = 0


Query: 9   LEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKNLIPL 68
           L++K+KQ LQ +E +TSNAD++QRQ L EIL+ NADVEYL++H LNG TD  TFKN++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  ISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSY 128
           I+YE ++P + RIANGD SPIL ++PI+EF  SSGTSGGE+KL+P  E E+ RR   +S 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VFAKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQS--GGDT 188
           +   M Q  P +  GK M+F F K+ SKT GG+ A  V TS+   +H  +         T
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196

Query: 189 SPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIRT 248
           SPN   LC+D YQS YSQ+LCGLC+++EVL++GA+FAS  +  +KFLE HW+ L  DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256

Query: 249 GTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTGS 308
           GT++  ITD SVRE++  IL+P+P+LADF+E EC K +W+GIITRLWPNTKY+  IVTG+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316

Query: 309 MSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVER 368
           MSQYIP LDYYSN LPL+   Y+SSEC+FG+NL PLCKP ++SYTLIP+MAYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376

Query: 369 GKD-----------DSKADKELVDLVDVKLGQEYELVITTYA--------------GFKN 428
                           K  +ELVDLVDVKLGQEYELV+TTYA              GFKN
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKN 436

Query: 429 KVPKLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGH 488
           K P+ SF+C+K+VVLS+D +KTDE EL N ++ A   L PF A++++YTSYAD+S++PGH
Sbjct: 437 KAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496

Query: 489 YVLYWEILCKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFE 548
           YVL+WE LC + ++ IP S++EDCCLAVEES N  YR+GR  +K+I PLEIKI+E GTF+
Sbjct: 497 YVLFWE-LCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556

Query: 549 KLMERALDRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRR 574
           KLM+ A+  GAS+ QYKTPRC+    IIE L+SR++ ++FS KCPKW P  +
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHK 607

[Close]

BLAST of Sed0017959 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 6.6e-198
Identity = 334/593 (56.32%), Postives = 435/593 (73.36%), Query Frame = 0


Query: 9   LEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKNLIPL 68
           L EK+K  LQ +E +T+NAD++QR+ L EILS NADVEYL++HGL G TD  TFK+++P+
Sbjct: 17  LAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPV 76

Query: 69  ISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSY 128
           ++YE ++P + RIANGD S +LC+ PI+EF  SSGTSGGE+KL+P  E E+ RR   +S 
Sbjct: 77  VTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VFAKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKN---SQSGGD 188
           +   M Q  P + KGK M+F F K+ SKT GG+ A  V TS+   +H FKN         
Sbjct: 137 LMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSH-FKNRPYDPYTNY 196

Query: 189 TSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIR 248
           TSPN   LC+D YQS YSQ+LCGLC+++EVL++GA+FAS  +  +KFLE HW  LA DIR
Sbjct: 197 TSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIR 256

Query: 249 TGTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTG 308
           TGT++ +ITDSSVRE++  IL+P+P+LADF+E+EC K +W+GIITRLWPNTKY+  IVTG
Sbjct: 257 TGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTG 316

Query: 309 SMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVE 368
           +MSQYIP LDYYSN LPL+   Y+SSEC+FG+NL PLCKP ++SYTLIPNMAYFEFLPV 
Sbjct: 317 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVH 376

Query: 369 RGKD-----------DSKADKELVDLVDVKLGQEYELVITTY--------------AGFK 428
           R                K  +ELVDLVDVKLGQEYELV+TTY              AGFK
Sbjct: 377 RNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 436

Query: 429 NKVPKLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPG 488
           N  P+ SF+C+K+VVLS+D +KTDE EL N ++ A   L PF A++++YTSYAD+S++PG
Sbjct: 437 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 496

Query: 489 HYVLYWEILCKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTF 548
           HYVL+WE LC   ++ IP S++EDCCL +EESLN  YR+GR  +K+I PLEIK++E GTF
Sbjct: 497 HYVLFWE-LCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 556

Query: 549 EKLMERALDRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRR 574
           +KLM+ A+  GAS+ QYKTPRC+    IIE L+SR++ ++FS KCPKW P  +
Sbjct: 557 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHK 607

[Close]

BLAST of Sed0017959 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 5.8e-170
Identity = 287/577 (49.74%), Postives = 400/577 (69.32%), Query Frame = 0


Query: 15  QTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTD-ASTFKNLIPLISYEQ 74
           + L+ +EH+T+NA ++QR+ L EIL+ NA  EYL+++G+ GS D    F+ L+PL++YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 75  LKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSYVFAKM 134
           L+P + RIANGD SPI   +PI+EF  SSGTSGGE+KL+P    E+ RR   +S +   M
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 135 KQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQS--GGDTSPNNV 194
            Q    + KGK M+  F K  S+T GG+ A  V TS+          +      TSP+  
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201

Query: 195 FLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIRTGTINP 254
            LC D YQS Y+QLLCGL    +VL++GA+FAS  +  + FLE HW  L +DIRTG ++P
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261

Query: 255 KITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTGSMSQYI 314
           +ITD  VR+++  +LR +P LAD IE EC++ +WEGII RLWP TKYI  IVTG+MSQYI
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321

Query: 315 PLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVERGKDDS 374
           P L++Y   LPL    Y+SSEC+FGLNL+P+CKP  ++YTLIP M Y+EFLPV      +
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381

Query: 375 KAD-KELVDLVDVKLGQEYELVITTY--------------AGFKNKVPKLSFVCKKDVVL 434
           +A  ++LVDLVDVKLG EYELV+TTY              AGFKNK P  SFV +++V L
Sbjct: 382 EASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVAL 441

Query: 435 SLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVLYWEILCKEQSST 494
           S+D +KTDE EL+  +  A + L+PFGA++ +YTSYAD++T+PGHYVL+WE+  +  S+ 
Sbjct: 442 SVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWEL--RAGSTA 501

Query: 495 IPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMERALDRGASMAQ 554
           +P+S++E+CCL+VEE+LN  YR+GR+ +++I PLEI+++ +GTF+KLM+ A+ RGAS+ Q
Sbjct: 502 VPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQ 561

Query: 555 YKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRR 574
           YK PRC+    ++E LD+R+   +FS KCPKW P  +
Sbjct: 562 YKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNK 596

[Close]

BLAST of Sed0017959 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 8.1e-164
Identity = 284/579 (49.05%), Postives = 393/579 (67.88%), Query Frame = 0


Query: 14  KQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDAS--TFKNLIPLISY 73
           ++ L+H+E +T NA + QR+ L EIL+ NA  EYL++ G+ G    +   F+ L PL++Y
Sbjct: 24  REKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLAPLVTY 83

Query: 74  EQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSYVFA 133
           E + P V RIANGD SPIL  +P++EF  SSGTSGGE+KL+P  E E+ RR   +S +  
Sbjct: 84  EDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMERRSGLYSLLMP 143

Query: 134 KMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQSGGD--TSPN 193
            M +  P + KGK M+  F K+  +T GG+ A  V TSF    +  +         TSP+
Sbjct: 144 VMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPHDPYTVYTSPD 203

Query: 194 NVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIRTGTI 253
              LC D YQS Y+QL+CGL    +VL++GA+FAS  +  ++FLE HW  L  DIR G +
Sbjct: 204 EAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSLCRDIRAGEL 263

Query: 254 NPKITDSSVRESIMNILR-PNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTGSMS 313
           +  +TD +VR ++  +LR  +P LAD IE EC++ +W+GII R+WP+TKYI  IVTG+M+
Sbjct: 264 DGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYIDVIVTGAMA 323

Query: 314 QYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVERGK 373
           QYIP L++Y   LPL    Y+SSEC+FGLNL+P+CKP +++YTLIP M YFEFLPV  G 
Sbjct: 324 QYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYFEFLPVNSGA 383

Query: 374 DDSKA----DKELVDLVDVKLGQEYELVITTY--------------AGFKNKVPKLSFVC 433
           +D  A     + LVDLVDVKLG EYELV+TTY              AGFKN  P  +FV 
Sbjct: 384 NDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNAAPMFAFVR 443

Query: 434 KKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYAD-SSTVPGHYVLYWEIL 493
           +K+V LS+D +KTDEAEL+  + EA + L+PFGA++ +YTSYAD ++T+PGHYVL+WE+ 
Sbjct: 444 RKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIPGHYVLFWELR 503

Query: 494 CKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMERALD 553
                + +P+S++EDCCLAVEE LN  YR+ R+ +++I PLEI+++ DGTF+KLM+ AL 
Sbjct: 504 SPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGTFDKLMDYALS 563

Query: 554 RGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKW 569
           RGAS+ QYK PRC+    ++E LD R+ + +FS KCPKW
Sbjct: 564 RGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKW 602

[Close]

BLAST of Sed0017959 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 576.6 bits (1485), Expect = 3.1e-163
Identity = 281/587 (47.87%), Postives = 395/587 (67.29%), Query Frame = 0


Query: 5   AQNLLEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKN 64
           A   ++E D + L+ ++ +T+N D +Q + L EIL  NA  EYL + GL+G+TD + F+ 
Sbjct: 18  AAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRA 77

Query: 65  LIPLISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLS 124
            +P++SY+ L+PY++RIANGD SPIL   P++EF  SSGTS GE+KL+P    E+ RR  
Sbjct: 78  KVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQL 137

Query: 125 FFSYVFAKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKN---SQ 184
            +S +   M    P + KGK ++F F K+ +KT GG+ A  V TS+    H FKN     
Sbjct: 138 LYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDH-FKNRPYDP 197

Query: 185 SGGDTSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLA 244
               TSP    LC D +QS Y+Q++CGLC+  +VL+LGA+FAS L+  ++FL+ +W  LA
Sbjct: 198 YHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLA 257

Query: 245 NDIRTGTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKA 304
           +DI +G + P++TD SVRE++  IL P+PELA  I  ECSKG W GIITR+WPNTKY+  
Sbjct: 258 DDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDV 317

Query: 305 IVTGSMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEF 364
           IVTG+M+QYIP L++YS  LP+    Y+SSEC+FGLNL P+C P ++SYT++PNM YFEF
Sbjct: 318 IVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEF 377

Query: 365 LPVERGKDDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVPKLS 424
           LPV+     S    +LVDL  V++G+EYELVITTYA              GF N  P+  
Sbjct: 378 LPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFR 437

Query: 425 FVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVLYWE 484
           FV +K+V+LS++ +KTDEAEL   ++ A+ LL P GA++ +YTS A +  +PGHYV+YWE
Sbjct: 438 FVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWE 497

Query: 485 ILCKEQSST-IPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMER 544
           +L K   +T + +     CCL +EE+LN  YR+ R  + +I PLEI+++  GTFE+LM+ 
Sbjct: 498 LLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDY 557

Query: 545 ALDRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRR 574
           A+ RGAS+ QYK PRC+    I+E LDSR++S+HFS   P W P+RR
Sbjct: 558 AISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

[Close]

BLAST of Sed0017959 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 1.0e-233
Identity = 394/580 (67.93%), Postives = 468/580 (80.69%), Query Frame = 0


Query: 9   LEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKNLIPL 68
           L+EKDKQ LQ LE +T  ADEIQR+ L EILSTNA VEYLQQHGLNGSTDASTFKNLIP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  ISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSY 128
           +SYE LKPY++RI NGDDSPILCA PITEF+ S+GTSGGEQKLIP  E+E  R+LS  +Y
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 VFAKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQSGGDTSP 188
           +  +MKQLFP +H GKEMFF F K  S TK GIVA SVSTSF    HL         TSP
Sbjct: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183

Query: 189 NNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIRTGT 248
            ++  CTD+YQS Y QLLCGLC+N++V+++G+LFASTL+ I KFLENHWVLLANDIRTGT
Sbjct: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243

Query: 249 INPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTGSMS 308
           IN KI DS VR+S+MNIL+P+P+LADFIE+ECSKGTWEGI+TRLWPNTK+I A+VTGSMS
Sbjct: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303

Query: 309 QYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVERGK 368
            YIP L+YY  +LPLI+ +Y+SSECFFG+NLDPLCKPE+ISYT IP +AYFEFLPV+R  
Sbjct: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363

Query: 369 DDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVPKLSFVCKKDV 428
           D  +  +E VDLVDVKLGQEYE+V+TTYA              GFKNK P   FV +K+V
Sbjct: 364 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 423

Query: 429 VLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVLYWEILCKEQS 488
           +LSLD+EKTDEAEL+N + +   LLS FGA + DYTSYADSST PGHYVLYWEI  KE+ 
Sbjct: 424 ILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEI 483

Query: 489 STIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMERALDRGASM 548
           S IPSSIYEDCCLA+EESL+  YRR RS +K INPLEIKI+E  TF+KLMER +D+GAS+
Sbjct: 484 SIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 543

Query: 549 AQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRRH 575
           +QYKTPRC+ S  IIEFLDS++++N+FS+K PKWDP+R H
Sbjct: 544 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583

[Close]

BLAST of Sed0017959 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.4e-230
Identity = 401/594 (67.51%), Postives = 474/594 (79.80%), Query Frame = 0


Query: 1   MSHDAQNLLEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDAS 60
           M+H  +   +E+DKQ LQH+E +T +A +IQRQ L +ILSTNADVEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLIPLISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIW 120
           TFKNLIPLISYEQL+PY++RIANGDDSPILC  PITEFFISSGTSGGE KLIP++E E  
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLSFFSYVFAKMKQLFPN--VHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFK 180
           RRLSFF YV  +MKQLFPN  V KGK M F+F K   KT+ GIVA++ +TS C R +L  
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANL-- 180

Query: 181 NSQSGGDTSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWV 240
                 +TSP ++ LCTD  QS Y QLLCGLC+N+ V ++GALFASTL+ +LKFLE+HWV
Sbjct: 181 -----NNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWV 240

Query: 241 LLANDIRTGTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKY 300
           +LA+DIR GT+N KITD SVRE++MNIL PN +LADFIE ECSKGTWEGIITRLWPN KY
Sbjct: 241 VLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKY 300

Query: 301 IKAIVTGSMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAY 360
           I AIVTGSM+QY+P L+YYSN+LP++S++Y +SECF GLNLDPLC P++ SYTLIP MAY
Sbjct: 301 INAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAY 360

Query: 361 FEFLPVERGKDDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVP 420
           FEFLP++R K   +  +ELVDLVDVKLG EYELVITTYA              GFKNK P
Sbjct: 361 FEFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAP 420

Query: 421 KLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVL 480
             SFVC+K+VVLSLDIEKTDEAEL++ +QEAA+LLSPFGA IADYTS+ADSS +PGHYVL
Sbjct: 421 NFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVL 480

Query: 481 YWEILCKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLM 540
           YWEI  K   S IPSSIYEDCC A+EE LN  YR  RS EK INPLEIKI++ GTFEKLM
Sbjct: 481 YWEIHVKGGIS-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLM 540

Query: 541 ERALDRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRRHRLFS 579
            +AL+RGASM+QYKTPRC+NS HIIE LDS ++  +FS+KCPK D    HRL S
Sbjct: 541 AQALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

[Close]

BLAST of Sed0017959 vs. ExPASy TrEMBL
Match: A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 2.3e-217
Identity = 373/579 (64.42%), Postives = 463/579 (79.97%), Query Frame = 0


Query: 5   AQNLLEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKN 64
           + ++L+ KDKQ L H+E  TSNADEIQR+ + EILS NA VEYL+Q GL+GSTD STFK 
Sbjct: 2   SDDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKK 61

Query: 65  LIPLISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLS 124
           LIP++SYEQLKPY++RIA+GDDSPILCA+ IT+F IS+GTSGG QKLIP+F+ E+ RRL+
Sbjct: 62  LIPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLA 121

Query: 125 FFSYVFAKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQ-SG 184
              Y    MKQLFP +  GKEMFF F +  +KTKGG+VA  +S S  ++  LF + +   
Sbjct: 122 TNYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLS-SCLSKCSLFSSVEVLR 181

Query: 185 GDTSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLAND 244
             TSP ++  CTD+YQS Y QLLCGLC+N+ VL++GALFASTL+ I KFLENHW LL ND
Sbjct: 182 AYTSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVND 241

Query: 245 IRTGTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIV 304
           IRTGT++ KI D  VRES+M++L+PNPELADFIE+ECSKG WEGI+TRLWPNTKYI  +V
Sbjct: 242 IRTGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVV 301

Query: 305 TGSMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLP 364
           TGSMSQYIP L++Y    P++S +Y+SSECFFG+NLDPLCKPE+ISYT +P MAYFEFLP
Sbjct: 302 TGSMSQYIPQLNFYC-KCPIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLP 361

Query: 365 VER-GKDDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVPKLSF 424
           V+R   ++ +  +ELVDLVDVKLGQEYE+V+TTYA              GFKNK P  SF
Sbjct: 362 VDRANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSF 421

Query: 425 VCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVLYWEI 484
            C+K+V+LSLDIEKTDE +L++ + EA+ LLSP GA I DYTSYADSST PGHYVLYWEI
Sbjct: 422 ACRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEI 481

Query: 485 LCKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMERAL 544
            C+E++STIPS++YEDCCLAVEESL+  YR+ RS  K INPLEIKI+E GTFEKLME+A+
Sbjct: 482 QCREETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAV 541

Query: 545 DRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPK 568
           DRGAS++QYKTPRC+ S  +IE LDS+++SN+FSQ+ PK
Sbjct: 542 DRGASISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

[Close]

BLAST of Sed0017959 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 8.6e-209
Identity = 364/589 (61.80%), Postives = 456/589 (77.42%), Query Frame = 0


Query: 1   MSHDAQNLLEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDAS 60
           MS+  +  L+EKDK+ LQ++E IT+ ADEIQRQ L EILS NA+VEYLQ+HGL+ STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 61  TFKNLIPLISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIW 120
           TFK LIPL+ Y+QL+PY+ RIA+GDDS ILC+ PITEFF SSGTSG E KLIP++E+E  
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 121 RRLSFFSYVFAKMKQLFPNV--HKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFK 180
           RRLSFFSY+  +MKQLFPN+  HK K + F F K   KTKGGIV  S+ T+   R+   +
Sbjct: 121 RRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLR 180

Query: 181 NSQSGGDTSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWV 240
           +  SG +TSP+++ LCTD YQS Y QLLCGL +N+ V ++ ALFASTL+ + KFLENHW+
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWI 240

Query: 241 LLANDIRTGTINPKITDSSVRESIMNI-LRPNPELADFIETECSKGTWEGIITRLWPNTK 300
            LA DIRT TINPKIT SSVRES+M I ++PNPE+AD IE EC KG WEGIITRLWPN K
Sbjct: 241 DLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 300

Query: 301 YIKAIVTGSMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMA 360
           YI A+VTGSMSQYIPLL+YY+NNLP++S++Y SSECF GLNLDPLC P+++SYTLIP MA
Sbjct: 301 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 360

Query: 361 YFEFLPVERGKDDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKV 420
           YFEFLP++      + ++ LVDLVDVKLG EYELVITT+A              GFKNK 
Sbjct: 361 YFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKA 420

Query: 421 PKLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYV 480
           P  SFV +K+VVL++  EKTDEA L+  ++E  +++  FGA I DYT+YAD ST+PGHYV
Sbjct: 421 PNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYV 480

Query: 481 LYWEIL---CKEQS-STIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGT 540
           LYWE+     KEQ+ + IP S++EDCCLA+E SLN+ YR  RS EK INPLEIKI++ GT
Sbjct: 481 LYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGT 540

Query: 541 FEKLMERALDRGASMAQYKTPRCINS--MHIIEFLDSRILSNHFSQKCP 567
           FEKLM+ AL+RGAS+ QYKTPRC+NS  +HII+ L+S ++SN+FS+  P
Sbjct: 541 FEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYP 589

[Close]

BLAST of Sed0017959 vs. ExPASy TrEMBL
Match: A0A1S3BJT8 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC103490488 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 8.6e-209
Identity = 364/589 (61.80%), Postives = 456/589 (77.42%), Query Frame = 0


Query: 1   MSHDAQNLLEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDAS 60
           MS+  +  L+EKDK+ LQ++E IT+ ADEIQRQ L EILS NA+VEYLQ+HGL+ STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 61  TFKNLIPLISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIW 120
           TFK LIPL+ Y+QL+PY+ RIA+GDDS ILC+ PITEFF SSGTSG E KLIP++E+E  
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 121 RRLSFFSYVFAKMKQLFPNV--HKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFK 180
           RRLSFFSY+  +MKQLFPN+  HK K + F F K   KTKGGIV  S+ T+   R+   +
Sbjct: 121 RRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLR 180

Query: 181 NSQSGGDTSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWV 240
           +  SG +TSP+++ LCTD YQS Y QLLCGL +N+ V ++ ALFASTL+ + KFLENHW+
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWI 240

Query: 241 LLANDIRTGTINPKITDSSVRESIMNI-LRPNPELADFIETECSKGTWEGIITRLWPNTK 300
            LA DIRT TINPKIT SSVRES+M I ++PNPE+AD IE EC KG WEGIITRLWPN K
Sbjct: 241 DLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 300

Query: 301 YIKAIVTGSMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMA 360
           YI A+VTGSMSQYIPLL+YY+NNLP++S++Y SSECF GLNLDPLC P+++SYTLIP MA
Sbjct: 301 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 360

Query: 361 YFEFLPVERGKDDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKV 420
           YFEFLP++      + ++ LVDLVDVKLG EYELVITT+A              GFKNK 
Sbjct: 361 YFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKA 420

Query: 421 PKLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYV 480
           P  SFV +K+VVL++  EKTDEA L+  ++E  +++  FGA I DYT+YAD ST+PGHYV
Sbjct: 421 PNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYV 480

Query: 481 LYWEIL---CKEQS-STIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGT 540
           LYWE+     KEQ+ + IP S++EDCCLA+E SLN+ YR  RS EK INPLEIKI++ GT
Sbjct: 481 LYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGT 540

Query: 541 FEKLMERALDRGASMAQYKTPRCINS--MHIIEFLDSRILSNHFSQKCP 567
           FEKLM+ AL+RGAS+ QYKTPRC+NS  +HII+ L+S ++SN+FS+  P
Sbjct: 541 FEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYP 589

[Close]

BLAST of Sed0017959 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 693.3 bits (1788), Expect = 1.6e-199
Identity = 334/592 (56.42%), Postives = 434/592 (73.31%), Query Frame = 0


Query: 9   LEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKNLIPL 68
           L++K+KQ LQ +E +TSNAD++QRQ L EIL+ NADVEYL++H LNG TD  TFKN++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  ISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSY 128
           I+YE ++P + RIANGD SPIL ++PI+EF  SSGTSGGE+KL+P  E E+ RR   +S 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VFAKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQS--GGDT 188
           +   M Q  P +  GK M+F F K+ SKT GG+ A  V TS+   +H  +         T
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196

Query: 189 SPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIRT 248
           SPN   LC+D YQS YSQ+LCGLC+++EVL++GA+FAS  +  +KFLE HW+ L  DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256

Query: 249 GTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTGS 308
           GT++  ITD SVRE++  IL+P+P+LADF+E EC K +W+GIITRLWPNTKY+  IVTG+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316

Query: 309 MSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVER 368
           MSQYIP LDYYSN LPL+   Y+SSEC+FG+NL PLCKP ++SYTLIP+MAYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376

Query: 369 GKD-----------DSKADKELVDLVDVKLGQEYELVITTYA--------------GFKN 428
                           K  +ELVDLVDVKLGQEYELV+TTYA              GFKN
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKN 436

Query: 429 KVPKLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGH 488
           K P+ SF+C+K+VVLS+D +KTDE EL N ++ A   L PF A++++YTSYAD+S++PGH
Sbjct: 437 KAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496

Query: 489 YVLYWEILCKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFE 548
           YVL+WE LC + ++ IP S++EDCCLAVEES N  YR+GR  +K+I PLEIKI+E GTF+
Sbjct: 497 YVLFWE-LCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556

Query: 549 KLMERALDRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRR 574
           KLM+ A+  GAS+ QYKTPRC+    IIE L+SR++ ++FS KCPKW P  +
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHK 607

[Close]

BLAST of Sed0017959 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 691.8 bits (1784), Expect = 4.7e-199
Identity = 334/593 (56.32%), Postives = 435/593 (73.36%), Query Frame = 0


Query: 9   LEEKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKNLIPL 68
           L EK+K  LQ +E +T+NAD++QR+ L EILS NADVEYL++HGL G TD  TFK+++P+
Sbjct: 17  LAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPV 76

Query: 69  ISYEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSY 128
           ++YE ++P + RIANGD S +LC+ PI+EF  SSGTSGGE+KL+P  E E+ RR   +S 
Sbjct: 77  VTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VFAKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKN---SQSGGD 188
           +   M Q  P + KGK M+F F K+ SKT GG+ A  V TS+   +H FKN         
Sbjct: 137 LMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSH-FKNRPYDPYTNY 196

Query: 189 TSPNNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIR 248
           TSPN   LC+D YQS YSQ+LCGLC+++EVL++GA+FAS  +  +KFLE HW  LA DIR
Sbjct: 197 TSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIR 256

Query: 249 TGTINPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTG 308
           TGT++ +ITDSSVRE++  IL+P+P+LADF+E+EC K +W+GIITRLWPNTKY+  IVTG
Sbjct: 257 TGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTG 316

Query: 309 SMSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVE 368
           +MSQYIP LDYYSN LPL+   Y+SSEC+FG+NL PLCKP ++SYTLIPNMAYFEFLPV 
Sbjct: 317 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVH 376

Query: 369 RGKD-----------DSKADKELVDLVDVKLGQEYELVITTY--------------AGFK 428
           R                K  +ELVDLVDVKLGQEYELV+TTY              AGFK
Sbjct: 377 RNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 436

Query: 429 NKVPKLSFVCKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPG 488
           N  P+ SF+C+K+VVLS+D +KTDE EL N ++ A   L PF A++++YTSYAD+S++PG
Sbjct: 437 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 496

Query: 489 HYVLYWEILCKEQSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTF 548
           HYVL+WE LC   ++ IP S++EDCCL +EESLN  YR+GR  +K+I PLEIK++E GTF
Sbjct: 497 HYVLFWE-LCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 556

Query: 549 EKLMERALDRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRR 574
           +KLM+ A+  GAS+ QYKTPRC+    IIE L+SR++ ++FS KCPKW P  +
Sbjct: 557 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHK 607

[Close]

BLAST of Sed0017959 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 575.1 bits (1481), Expect = 6.4e-164
Identity = 281/582 (48.28%), Postives = 389/582 (66.84%), Query Frame = 0


Query: 11  EKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKNLIPLIS 70
           EKD + L+ +E +T NAD +Q   L EIL+ NAD EYL++  L G+TD  TFK  IP+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 71  YEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSYVF 130
           YE L+P ++RIA+GD SPIL A PI+EF  SSGTS GE+KL+P  + E+ RR   +S + 
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 131 AKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQSGGD--TSP 190
             M    P + KGK M+F F K+ +KT GG+ A  V TS+    H         +  TSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 191 NNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIRTGT 250
           N   LC D +QS Y+Q+LCGL +   VL++GA+FAS L+  ++FL+ HW   A+DI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 251 INPKITDSSVRESIMNILRPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTGSMS 310
           ++ +ITD S+R+ +  IL+P+P LA+FI  EC    WE IITR+WPNTKY+  IVTG+M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 311 QYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVERGK 370
           QYIP L+YYS  LP+    Y+SSEC+FGLNL+P+ KP ++SYT++PNMAYFEF+P+    
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPL---- 376

Query: 371 DDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVPKLSFVCKKDV 430
                  + V+LVDV +G+EYELV+TTYA              GF N  P+  FV +K+V
Sbjct: 377 ----GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNV 436

Query: 431 VLSLDIEKTDEAELYNGIQEAAKLL-SPFGANIADYTSYADSSTVPGHYVLYWEILCKEQ 490
           +LS+D +KTDE+EL   ++ A+ +L    G+ +A+YTSYAD+ST+PGHYVLYWE+L ++ 
Sbjct: 437 LLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDG 496

Query: 491 SSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMERALDRGAS 550
           +          CCL +EESLN  YR+ R  + ++ PLEI+++ +GTFE+LM+ A+ RGAS
Sbjct: 497 ARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGAS 556

Query: 551 MAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRRHR 576
           + QYK PRC+N   I+E LDSR++S HFS   P W P RR R
Sbjct: 557 INQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRRR 590

[Close]

BLAST of Sed0017959 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 570.9 bits (1470), Expect = 1.2e-162
Identity = 283/581 (48.71%), Postives = 398/581 (68.50%), Query Frame = 0


Query: 12  KDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKNLIPLISY 71
           KD + L+ +E +T N D +Q++ + EILS N+D EYL++ GL G TD  TFK  +P++ Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 72  EQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSYVFA 131
           + LKP ++RIANGD S IL + PITEF  SSGTS GE+KL+P  + ++ RR   +S +  
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 132 KMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFK---NSQSGGDTSP 191
            M    P + KGK ++F F KT SKT GG+ A  V TS+  ++  FK   N      TSP
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSY-YKSEQFKRRPNDPYNVYTSP 197

Query: 192 NNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIRTGT 251
           N   LC D  QS Y+Q+LCGL    EVL+LGA+FAS L+  + FL+ +W  LA+DI TGT
Sbjct: 198 NEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGT 257

Query: 252 INPKITDSSVRESIMNIL-RPNPELADFIETECSK-GTWEGIITRLWPNTKYIKAIVTGS 311
           ++ +I+D +++ES+  IL +P+ ELADFI + C +  +WEGIIT++WPNTKY+  IVTG+
Sbjct: 258 LSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGA 317

Query: 312 MSQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVER 371
           M+QYIP+L+YYS  LP+    Y+SSE +FG+NL P+CKP ++SYT++PNMAYFEFLP   
Sbjct: 318 MAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHHE 377

Query: 372 GKDDSKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVPKLSFVCKK 431
              +     ELV+L DV++G+EYELVITTYA              GF N  P+  FV +K
Sbjct: 378 VPTEK---SELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRRK 437

Query: 432 DVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVLYWEILCKE 491
           +V+LS++ +KTDEAEL + ++ A+ LL   G  + +YTSYA++ T+PGHYV+YWE+L K+
Sbjct: 438 NVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLVKD 497

Query: 492 QSSTIPSSIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMERALDRGA 551
           Q++     +   CCL +EESLN  YR+ R  +K+I PLEI+++++GTFE+LM+ A+ RGA
Sbjct: 498 QTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISRGA 557

Query: 552 SMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRR 574
           S+ QYK PRC++   I+E LDSR++S HFS   P W P RR
Sbjct: 558 SINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERR 594

[Close]

BLAST of Sed0017959 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 567.0 bits (1460), Expect = 1.7e-161
Identity = 277/585 (47.35%), Postives = 396/585 (67.69%), Query Frame = 0


Query: 11  EKDKQTLQHLEHITSNADEIQRQTLVEILSTNADVEYLQQHGLNGSTDASTFKNLIPLIS 70
           EKD + L+ +E +T N D +Q + L EIL+ N++ EYL++  L+G  D  TFK+ +P+++
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 71  YEQLKPYVERIANGDDSPILCAEPITEFFISSGTSGGEQKLIPIFEREIWRRLSFFSYVF 130
           YE LKP ++RI+NGD SPIL + PITEF  SSGTS GE+KL+P  E ++ RR   +S + 
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 131 AKMKQLFPNVHKGKEMFFRFNKTGSKTKGGIVANSVSTSFCNRTHLFKNSQSGGD--TSP 190
             M    P + KGK ++F F K+ SKT GG+ A  V TS+    H  +      +  TSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 191 NNVFLCTDLYQSTYSQLLCGLCENEEVLKLGALFASTLVSILKFLENHWVLLANDIRTGT 250
           N   LC+D  QS Y+Q+LCGL    EVL+LGA+FAS L+  + FL+N+W  LA DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 251 INPKITDSSVRESIMNIL-RPNPELADFIETECSKGTWEGIITRLWPNTKYIKAIVTGSM 310
           ++ +I D +++  +  IL +P+ ELA+F+   CS+  WEGIIT++WPNTKY+  IVTG+M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317

Query: 311 SQYIPLLDYYSNNLPLISEYYSSSECFFGLNLDPLCKPEKISYTLIPNMAYFEFLPVERG 370
           +QYIP L+YYS  LP+    Y+SSE +FG+NL P+CKP ++SYT++PNMAYFEFLP    
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHD 377

Query: 371 KDD----SKADKELVDLVDVKLGQEYELVITTYA--------------GFKNKVPKLSFV 430
            D     S  +  LV+L +V++G+EYELVITTYA              GF N  P+  F+
Sbjct: 378 GDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFI 437

Query: 431 CKKDVVLSLDIEKTDEAELYNGIQEAAKLLSPFGANIADYTSYADSSTVPGHYVLYWEIL 490
            +K+V+LS++ +KTDEAEL   ++ A++L +  G  + +YTSYA++ T+PGHYV+YWE+L
Sbjct: 438 RRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWELL 497

Query: 491 CKEQSSTIPS-SIYEDCCLAVEESLNVFYRRGRSQEKNINPLEIKILEDGTFEKLMERAL 550
            ++QS+ + S  +   CCL +EESLN  YR+ R  +K+I PLEI+++ +GTFE+LM+ A+
Sbjct: 498 GRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDYAI 557

Query: 551 DRGASMAQYKTPRCINSMHIIEFLDSRILSNHFSQKCPKWDPSRR 574
            RGAS+ QYK PRC++   I+E LDSR++S HFS   P W P RR
Sbjct: 558 SRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERR 602

The following BLAST results are available for this feature:

BLAST of Sed0017959 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140778.1	2.1e-233	67.93	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]
XP_022944596.1	2.8e-230	67.51	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
KAG6570863.1	3.7e-230	67.34	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_038902067.1	6.5e-219	64.09	indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida]	[more]
XP_022140368.1	4.7e-217	64.42	indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia]	[more]

BLAST of Sed0017959 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81829	2.3e-198	56.42	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LSQ4	6.6e-198	56.32	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	5.8e-170	49.74	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	8.1e-164	49.05	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
A3BLS0	3.1e-163	47.87	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399...	[more]

BLAST of Sed0017959 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIS9	1.0e-233	67.93	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1FUU5	1.4e-230	67.51	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1CHU9	2.3e-217	64.42	indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 ...	[more]
A0A5A7V3L5	8.6e-209	61.80	Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BJT8	8.6e-209	61.80	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of Sed0017959 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27260.1	1.6e-199	56.42	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	4.7e-199	56.32	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	6.4e-164	48.28	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	1.2e-162	48.71	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	1.7e-161	47.35	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 20..545 e-value: 2.2E-170 score: 568.0
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 9..574
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 9..574

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0017959.1	Sed0017959.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity